Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0007519

Physical Location & Seq

Genomic Coordinates Forward (+)

41832913 .. 41839373

Gene Structure 6461 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG317400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGAGTGCCCTCAAGATCACCAAAAAGCACCACAAACACTTGAACAATCCTTTCCCTTCCGCACCTCTCTCTCTCCCGCTCATCCAAGGAAACCTTGTCTTTAATTCTCAAACGGTGCCTTCACCCCAACGTTTTGATATTGGAAAGGATTTTGAGCTGTCTTGGAACACCAGCAATGGCGGGTCTTTGTCAATTTCTCACCGATCGCAGCCCAGGAGAGCCATATGGTCCACCGTTCCTGGTCAAGCCTTTGTCTCTGCAGCAGTGGCTCAAACACGAGTGGAAGAAAGCAGGGGTTCTTTTGTGGTCGAAGACATTTGTGTTGATCTGGTTTGCCATCACCAAACCATTCAAGACATAAGGGTGATCAGTCAGTCTGATTCTCCATCTGGGTTTTCAGGGCTGGACCTGGAATCAGATTCTCCAGGTACCCAATTCCCTATTGTCCTTGTAACTGGCTGGGTTTTCAATGCGGAGAGGAGGAAGAAGCGTTTTCTGAAATCTGGGAATCCCAAATTTGAGGGGAAGGGGTCTTCTGCTTGTGCAAAGTATCGGGTTTTGTTTGAACAGAAATGCAGTCATCAAATTGGGTTCCAAGTGAAGCTTGGGCAACCCAACTTTGAATTTCCCACCAAAGCAGCTACTTACAAGGGTTTTAGAACTAGGCTGCTGGGTCGGTTTCAGAAACCCAGGCTGCGGTGGTCTTGGTCCTTTACGAGGCCCAGAGGGTTTGTCTTGCTTCCCTCTGAGGCTCAACAATTCAAAGAATTCAACAGGGTGTGCCTGACATATTCAAGTGAAGAAAAGGAGAGGTTTTATGGATTTGGGGAGCAATTCTCTTATATGGATTTTAAGGGCAAAAGGGTGCCCATTTTTGTTCAGGAACAGGGTATTGGGAGAGGAGATCAGCCTATTACCTTTGCAGCTAACTTAGTCAGTTACAGGGCTGGCGGCGATTGGAGTACAACCTATGCTCCTTCCCCATTCTATATGACTTCAAAGATGAAATCTCTTTACCTTGAAGGATATGATTATTGCGTATTTGATCTAACACAACATGACAAAGTTCAGATAAAGATACATGGGAATTCAGTAGAGGGACGGATATTGCATGGAAACTCACCGACTGAGCTCATTGAATGTTACACTGAAACCATTGGGAGACCTCCTAAACTTCCAGACTGGATTATTTCTGGAGCTGTTGTTGGCATGCAGGGTGGCACAAAATCTGTACGTCGTATTTGGAATGAGCTTAAGAGTTACAATGCTCCAGTTTCAGCATTCTGGTTGCAGGATTGGGTGGGCCACAGAGAGACATTGGTTGGATCACAACTCTGGTGGAATTGGGAAGTAGATTCAACAAGGTATATGGGATGGCAACAACTAATTAAAGATCTTAGTGCTCAGCATATTAAAGTGATGACATACTGCAACCCATGTCTAGCTCCGTGTCATGAAAAGCCAAACAGAAGGAGAAACCTCTTTGAGGAAGCAAAGAAGTTGGACATATTAGTGAAAGACAAGCAAGGAGAACCTTATATGGTTCCAAATACTGCTTTTGATGTAGGAATGTTGGACTTGACACATCCAGATACTGGAAATTGGTTCAAGCAGATTCTTCAGGAAATGGTTGATGATGGAGTCAGAGGATGGATGGCTGATTTTGGTGAAGGCCTGCCTGTGGATGCCACCCTCTATTCAGGCGAAGATCCTATTTCAGCCCACAACAAGTACCCAGAGCTATGGGCCCAAATAAATAGAGAGTTTGTGGAAGAATGGAAAGCTAACCGTGTGGGAAAGGAGAAAGAAGATCCACAAGAGGCGTTGGTCTTTTTCATGAGGGCTGGTTTCAGAGATAGCCCCAAATGGGGAATGCTATTTTGGGAAGGAGATCAGATGGTAAGTTGGCAGATTCATGACGGAATAAAGAGTGCTGTTGTTGGTCTACTCAGCAGTGGAATTTCAGGGTATGCTTTCAATCACAGTGATATTGGAGGGTACTGTGCTGTAAATTTACCTTTCATCAAGTATCGAAGAAGTGAAGAATTGCTTTTGCGATGGATGGAGCTAAATGCTTTCACCACTGTTTTCCGGACACATGAAGGAAACAAACCATCTTGCAACAGTCAATTCTACTCAAATGAGAGAACTCTAGCTCATTTTGCACGATTTGCTAAAGTCTATATAGCTTGGAAGTTCTATAGGATCCAGCTCATCGAGGAAGCGACTCATAGAGGCCTGCCTGTATGCCGCCACTTATTTCTGCATTACCCTAATGATGAACATGTTCATAATTTGAGTTACCAGCAGTTCTTGATTGGTACTGAGATTCTGGTGGTGCCTGTCCTCGACAAAGGTATGAGCAATGTGAAGGCTTATTTTCCAACTGGTAATGACTGTTGGCAACATGTATGGACTGGAAAGCAGTTTACTGAAGAGGGTTTTGAAACCATAGTAGAAGCTCAAATTGGTTACCCTGCTGTATTTGTTAAGACTGGTTCCGTTGTTGGAGAGACGTTTTTGAAAAACCTCAGAGATTTGAAAATTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_31

Protein Analysis

850

Amino Acids

97.24

Weight (kDa)

8.6

Isoelectric Point (pI)

46.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_31_2nd	PF01055	407 - 716	7.8e-37	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	746 - 836	3.6e-16	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007519)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360
malus_domestica	MD14G1090500.v1.1
prunus_persica	Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1
pyrus_communis	pycom14g08100
rosa_chinensis	RchiOBHm_Chr2g0125791
rosa_laevigata	RLG00000018880
rosa_multiflora	Rmu_sc0004466.1_g000001 Rmu_ssc0000055.1_g000006
rosa_roxburghii	Rroxscaffold_2G00117700
rosa_rugosa	Rorug02G0259300
rosa_samantha	Rh2AG317400 Rh2BG325500 Rh2CG307200 Rh2DG343400
rosa_wichuraiana	Rw2G025680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	428
AccB1I	GGYRCC	4 cut(s)	115, 428, 865, 2338
AccB7I	CCANNNNNTGG	1 cut(s)	1595
AccBSI	CCGCTC	1 cut(s)	79
AccI	GTMKAC	1 cut(s)	1945
AccIII	TCCGGA	1 cut(s)	2091
AciI	CCGC	7 cut(s)	60, 77, 180, 473, 697, 951, 2251
AclI	AACGTT	1 cut(s)	130
AclWI	GGATC	5 cut(s)	1333, 1703, 1805, 2200, 2213
AcsI	RAATTY	7 cut(s)	515, 623, 767, 1087, 1959, 2011, 2544
AcuI	CTGAAG	2 cut(s)	1604, 2454
AfaI	GTAC	6 cut(s)	430, 964, 1233, 1733, 2000, 2323
AfiI	CCNNNNNNNGG	7 cut(s)	696, 717, 1486, 1595, 1681, 1867, 1868
AflII	CTTAAG	1 cut(s)	1253
AflIII	ACRYGT	2 cut(s)	2284, 2407
AjnI	CCWGG	5 cut(s)	212, 238, 408, 424, 689
AjuI	GAANNNNNNNTTGG	6 cut(s)	876, 908, 1864, 1896, 2451, 2483
Alw21I	GWGCWC	2 cut(s)	1134, 1405
Alw26I	GTCTC	4 cut(s)	259, 1156, 1307, 2507
AlwI	GGATC	5 cut(s)	1333, 1703, 1805, 2200, 2213
AlwNI	CAGNNNCTG	1 cut(s)	1595
Aor13HI	TCCGGA	1 cut(s)	2091
AoxI	GGCC	5 cut(s)	719, 1303, 1672, 1746, 2236
ApaI	GGGCCC	1 cut(s)	1750
ApeKI	GCWGC	6 cut(s)	208, 260, 638, 667, 694, 923
ApoI	RAATTY	7 cut(s)	515, 623, 767, 1087, 1959, 2011, 2544
Asp700I	GAANNNNTTC	1 cut(s)	2286
Asp718I	GGTACC	1 cut(s)	428
AspS9I	GGNCC	7 cut(s)	228, 406, 708, 720, 1303, 1746, 1747
AsuHPI	GGTGA	8 cut(s)	10, 114, 191, 332, 376, 1113, 1679, 2071
AvaII	GGWCC	3 cut(s)	228, 406, 708
BaeGI	GKGCMC	3 cut(s)	10, 870, 1750
BamHI	GGATCC	1 cut(s)	2205
BanI	GGYRCC	4 cut(s)	115, 428, 865, 2338
BanII	GRGCYC	2 cut(s)	1134, 1750
BauI	CACGAG	1 cut(s)	276
BbsI	GAAGAC	2 cut(s)	318, 525
Bbv12I	GWGCWC	2 cut(s)	1134, 1405
BbvI	GCAGC	6 cut(s)	220, 272, 650, 654, 681, 935
BciT130I	CCWGG	5 cut(s)	214, 240, 410, 426, 691
BclI	TGATCA	1 cut(s)	366
BcoDI	GTCTC	4 cut(s)	259, 1156, 1307, 2507
BfaI	CTAG	3 cut(s)	663, 1442, 2153
BfmI	CTRYAG	2 cut(s)	258, 2200
BfrI	CTTAAG	1 cut(s)	1253
BglII	AGATCT	1 cut(s)	1393
BisI	GCNGC	8 cut(s)	209, 261, 639, 668, 695, 924, 952, 2251
BlpI	GCTNAGC	1 cut(s)	1404
BlsI	GCNGC	8 cut(s)	210, 262, 640, 669, 696, 925, 953, 2252
Bme1390I	CCNGG	5 cut(s)	214, 240, 410, 426, 691
Bme18I	GGWCC	3 cut(s)	228, 406, 708
BmgT120I	GGNCC	7 cut(s)	228, 406, 708, 720, 1303, 1746, 1747
BmiI	GGNNCC	9 cut(s)	117, 430, 593, 867, 1545, 1748, 2207, 2340, 2500
BmrFI	CCNGG	5 cut(s)	214, 240, 410, 426, 691
BmsI	GCATC	1 cut(s)	1675
BpiI	GAAGAC	2 cut(s)	318, 525
BplI	GAGNNNNNCTC	2 cut(s)	821, 853
BpmI	CTGGAG	3 cut(s)	408, 1215, 1254
Bpu1102I	GCTNAGC	1 cut(s)	1404
BsaI	GGTCTC	1 cut(s)	1156
BsaJI	CCNNGG	3 cut(s)	85, 212, 689
BsaWI	WCCGGW	1 cut(s)	2091
BsaXI	ACNNNNNCTCC	4 cut(s)	958, 988, 1188, 1218
Bsc4I	CCNNNNNNNGG	7 cut(s)	696, 717, 1486, 1595, 1681, 1867, 1868
Bse1I	ACTGG	7 cut(s)	460, 1187, 1271, 1600, 2392, 2422, 2500
Bse3DI	GCAATG	2 cut(s)	181, 2371
BseAI	TCCGGA	1 cut(s)	2091
BseBI	CCWGG	5 cut(s)	214, 240, 410, 426, 691
BseDI	CCNNGG	3 cut(s)	85, 212, 689
BseGI	GGATG	7 cut(s)	81, 1379, 1585, 1655, 1659, 1690, 2067
BseLI	CCNNNNNNNGG	7 cut(s)	696, 717, 1486, 1595, 1681, 1867, 1868
BseMI	GCAATG	2 cut(s)	181, 2371
BseMII	CTCAG	6 cut(s)	738, 1119, 1418, 1963, 2316, 2545
BseNI	ACTGG	7 cut(s)	460, 1187, 1271, 1600, 2392, 2422, 2500
BseRI	GAGGAG	2 cut(s)	493, 915
BseSI	GKGCMC	3 cut(s)	10, 870, 1750
BseXI	GCAGC	6 cut(s)	220, 272, 650, 654, 681, 935
BseYI	CCCAGC	2 cut(s)	459, 670
Bsh1285I	CGRYCG	1 cut(s)	206
BshFI	GGCC	5 cut(s)	721, 1305, 1674, 1748, 2238
BshNI	GGYRCC	4 cut(s)	115, 428, 865, 2338
BsiEI	CGRYCG	1 cut(s)	206
BsiHKAI	GWGCWC	2 cut(s)	1134, 1405
BsiSI	CCGG	1 cut(s)	2092
BslFI	GGGAC	1 cut(s)	1113
BslI	CCNNNNNNNGG	7 cut(s)	696, 717, 1486, 1595, 1681, 1867, 1868
BsmAI	GTCTC	4 cut(s)	259, 1156, 1307, 2507
BsmBI	CGTCTC	1 cut(s)	2507
BsmFI	GGGAC	1 cut(s)	1113
BsmI	GAATGC	2 cut(s)	1280, 1878
BsnI	GGCC	5 cut(s)	721, 1305, 1674, 1748, 2238
Bso31I	GGTCTC	1 cut(s)	1156
Bsp120I	GGGCCC	1 cut(s)	1746
Bsp1286I	GDGCHC	5 cut(s)	10, 870, 1134, 1405, 1750
Bsp13I	TCCGGA	1 cut(s)	2091
Bsp1720I	GCTNAGC	1 cut(s)	1404
BspACI	CCGC	7 cut(s)	60, 77, 180, 473, 697, 951, 2251
BspANI	GGCC	5 cut(s)	721, 1305, 1674, 1748, 2238
BspCNI	CTCAG	6 cut(s)	739, 1120, 1417, 1962, 2317, 2544
BspEI	TCCGGA	1 cut(s)	2091
BspHI	TCATGA	3 cut(s)	1453, 1836, 1915
BspLI	GGNNCC	9 cut(s)	117, 430, 593, 867, 1545, 1748, 2207, 2340, 2500
BspMAI	CTGCAG	1 cut(s)	262
BspPI	GGATC	5 cut(s)	1333, 1703, 1805, 2200, 2213
BspT107I	GGYRCC	4 cut(s)	115, 428, 865, 2338
BspTI	CTTAAG	1 cut(s)	1253
BspTNI	GGTCTC	1 cut(s)	1156
BsrBI	CCGCTC	1 cut(s)	79
BsrDI	GCAATG	2 cut(s)	181, 2371
BsrI	ACTGG	7 cut(s)	460, 1187, 1271, 1600, 2392, 2422, 2500
BssECI	CCNNGG	3 cut(s)	85, 212, 689
BssSI	CACGAG	1 cut(s)	276
BssT1I	CCWWGG	1 cut(s)	85
Bst2BI	CACGAG	1 cut(s)	276
Bst2UI	CCWGG	5 cut(s)	214, 240, 410, 426, 691
Bst4CI	ACNGT	8 cut(s)	115, 235, 1790, 1985, 2003, 2086, 2126, 2399
Bst6I	CTCTTC	1 cut(s)	2430
BstAFI	CTTAAG	1 cut(s)	1253
BstAPI	GCANNNNNTGC	1 cut(s)	2171
BstC8I	GCNNGC	4 cut(s)	949, 1211, 1676, 2240
BstDEI	CTNAG	8 cut(s)	747, 931, 1128, 1397, 1404, 1949, 2325, 2531
BstEII	GGTNACC	1 cut(s)	2471
BstENI	CCTNNNNNAGG	2 cut(s)	715, 1484
BstF5I	GGATG	7 cut(s)	81, 1379, 1585, 1655, 1659, 1690, 2067
BstMAI	GTCTC	4 cut(s)	259, 1156, 1307, 2507
BstMCI	CGRYCG	1 cut(s)	206
BstMWI	GCNNNNNNNGC	3 cut(s)	266, 2162, 2171
BstNI	CCWGG	5 cut(s)	214, 240, 410, 426, 691
BstNSI	RCATGY	3 cut(s)	1213, 2288, 2411
BstPI	GGTNACC	1 cut(s)	2471
BstSCI	CCNGG	5 cut(s)	212, 238, 408, 424, 689
BstSFI	CTRYAG	2 cut(s)	258, 2200
BstSLI	GKGCMC	3 cut(s)	10, 870, 1750
BstV1I	GCAGC	6 cut(s)	220, 272, 650, 654, 681, 935
BstV2I	GAAGAC	2 cut(s)	318, 525
BstX2I	RGATCY	4 cut(s)	1393, 1708, 1810, 2205
BstXI	CCANNNNNNTGG	1 cut(s)	1743
BstYI	RGATCY	4 cut(s)	1393, 1708, 1810, 2205
BsuRI	GGCC	5 cut(s)	721, 1305, 1674, 1748, 2238
BtgZI	GCGATG	1 cut(s)	2071
BtsCI	GGATG	7 cut(s)	81, 1379, 1585, 1655, 1659, 1690, 2067
BtsI	GCAGTG	2 cut(s)	270, 1960
BtsIMutI	CAGTG	5 cut(s)	270, 1146, 1960, 1990, 2082
Cac8I	GCNNGC	4 cut(s)	949, 1211, 1676, 2240
CaiI	CAGNNNCTG	1 cut(s)	1595
CciI	TCATGA	3 cut(s)	1453, 1836, 1915
Cfr13I	GGNCC	7 cut(s)	228, 406, 708, 720, 1303, 1746, 1747
Csp6I	GTAC	6 cut(s)	429, 963, 1232, 1732, 1999, 2322
CspCI	CAANNNNNGTGG	2 cut(s)	1319, 1354
CviQI	GTAC	6 cut(s)	429, 963, 1232, 1732, 1999, 2322
DdeI	CTNAG	8 cut(s)	747, 931, 1128, 1397, 1404, 1949, 2325, 2531
Eam1104I	CTCTTC	1 cut(s)	2430
EarI	CTCTTC	1 cut(s)	2430
Ecl136II	GAGCTC	1 cut(s)	1132
Eco130I	CCWWGG	1 cut(s)	85
Eco147I	AGGCCT	2 cut(s)	1674, 2238
Eco24I	GRGCYC	2 cut(s)	1134, 1750
Eco31I	GGTCTC	1 cut(s)	1156
Eco47I	GGWCC	3 cut(s)	228, 406, 708
Eco53kI	GAGCTC	1 cut(s)	1132
Eco57I	CTGAAG	2 cut(s)	1604, 2454
Eco91I	GGTNACC	1 cut(s)	2471
EcoICRI	GAGCTC	1 cut(s)	1132
EcoNI	CCTNNNNNAGG	2 cut(s)	715, 1484
EcoO65I	GGTNACC	1 cut(s)	2471
EcoRI	GAATTC	2 cut(s)	767, 1087
EcoRII	CCWGG	5 cut(s)	212, 238, 408, 424, 689
EcoT14I	CCWWGG	1 cut(s)	85
EcoT38I	GRGCYC	2 cut(s)	1134, 1750
ErhI	CCWWGG	1 cut(s)	85
Esp3I	CGTCTC	1 cut(s)	2507
FalI	AAGNNNNNCTT	6 cut(s)	78, 110, 588, 620, 2034, 2066
FaqI	GGGAC	1 cut(s)	1113
FauI	CCCGC	2 cut(s)	84, 173
FauNDI	CATATG	1 cut(s)	224
FbaI	TGATCA	1 cut(s)	366
FblI	GTMKAC	1 cut(s)	1945
Fnu4HI	GCNGC	8 cut(s)	209, 261, 639, 668, 695, 924, 952, 2251
FokI	GGATG	7 cut(s)	68, 1386, 1572, 1662, 1666, 1697, 2074
FriOI	GRGCYC	2 cut(s)	1134, 1750
Fsp4HI	GCNGC	8 cut(s)	209, 261, 639, 668, 695, 924, 952, 2251
FspBI	CTAG	3 cut(s)	663, 1442, 2153
GluI	GCNGC	8 cut(s)	209, 261, 639, 668, 695, 924, 952, 2251
GsaI	CCCAGC	2 cut(s)	463, 674
GsuI	CTGGAG	3 cut(s)	408, 1215, 1254
HaeIII	GGCC	5 cut(s)	721, 1305, 1674, 1748, 2238
HapII	CCGG	1 cut(s)	2092
HindIII	AAGCTT	1 cut(s)	602
HpaII	CCGG	1 cut(s)	2092
HphI	GGTGA	8 cut(s)	10, 114, 191, 332, 376, 1113, 1679, 2071
Hpy166II	GTNNAC	3 cut(s)	231, 1946, 2430
Hpy8I	GTNNAC	3 cut(s)	231, 1946, 2430
Hpy99I	CGWCG	1 cut(s)	1239
HpyCH4III	ACNGT	8 cut(s)	115, 235, 1790, 1985, 2003, 2086, 2126, 2399
HpyCH4IV	ACGT	3 cut(s)	130, 1234, 2516
HpyF10VI	GCNNNNNNNGC	3 cut(s)	266, 2162, 2171
HpyF3I	CTNAG	8 cut(s)	747, 931, 1128, 1397, 1404, 1949, 2325, 2531
HpySE526I	ACGT	3 cut(s)	130, 1234, 2516
Kpn2I	TCCGGA	1 cut(s)	2091
KpnI	GGTACC	1 cut(s)	432
Ksp22I	TGATCA	1 cut(s)	366
LmnI	GCTCC	6 cut(s)	829, 979, 1196, 1273, 1450, 2065
Lsp1109I	GCAGC	6 cut(s)	220, 272, 650, 654, 681, 935
LweI	GCATC	1 cut(s)	1675
MaeI	CTAG	3 cut(s)	663, 1442, 2153
MaeII	ACGT	3 cut(s)	130, 1234, 2516
MaeIII	GTNAC	6 cut(s)	451, 938, 1142, 1259, 2300, 2471
MbiI	CCGCTC	1 cut(s)	79
MflI	RGATCY	4 cut(s)	1393, 1708, 1810, 2205
MhlI	GDGCHC	5 cut(s)	10, 870, 1134, 1405, 1750
MlyI	GAGTC	2 cut(s)	1650, 2221
MmeI	TCCRAC	5 cut(s)	1303, 1482, 1554, 2408, 2488
MroI	TCCGGA	1 cut(s)	2091
MroXI	GAANNNNTTC	1 cut(s)	2286
MseI	TTAA	7 cut(s)	102, 852, 1254, 1389, 1413, 2490, 2551
MslI	CAYNNNNRTG	2 cut(s)	362, 1511
MspCI	CTTAAG	1 cut(s)	1253
MspI	CCGG	1 cut(s)	2092
MspR9I	CCNGG	5 cut(s)	214, 240, 410, 426, 691
Mva1269I	GAATGC	2 cut(s)	1280, 1878
MvaI	CCWGG	5 cut(s)	214, 240, 410, 426, 691
MwoI	GCNNNNNNNGC	3 cut(s)	266, 2162, 2171
NdeI	CATATG	1 cut(s)	224
NlaIV	GGNNCC	9 cut(s)	117, 430, 593, 867, 1545, 1748, 2207, 2340, 2500
NspI	RCATGY	3 cut(s)	1213, 2288, 2411
PaeI	GCATGC	1 cut(s)	1213
PagI	TCATGA	3 cut(s)	1453, 1836, 1915
PceI	AGGCCT	2 cut(s)	1674, 2238
PciI	ACATGT	2 cut(s)	2284, 2407
PctI	GAATGC	2 cut(s)	1280, 1878
PdmI	GAANNNNTTC	1 cut(s)	2286
PfeI	GAWTC	8 cut(s)	380, 413, 419, 508, 1355, 1615, 1912, 2329
PflMI	CCANNNNNTGG	1 cut(s)	1595
PkrI	GCNGC	8 cut(s)	210, 262, 640, 669, 696, 925, 953, 2252
Ple19I	CGATCG	1 cut(s)	206
PleI	GAGTC	2 cut(s)	1649, 2221
PpsI	GAGTC	2 cut(s)	1649, 2221
PscI	ACATGT	2 cut(s)	2284, 2407
Psp124BI	GAGCTC	1 cut(s)	1134
Psp1406I	AACGTT	1 cut(s)	130
Psp6I	CCWGG	5 cut(s)	212, 238, 408, 424, 689
PspEI	GGTNACC	1 cut(s)	2471
PspFI	CCCAGC	2 cut(s)	459, 670
PspGI	CCWGG	5 cut(s)	212, 238, 408, 424, 689
PspN4I	GGNNCC	9 cut(s)	117, 430, 593, 867, 1545, 1748, 2207, 2340, 2500
PspOMI	GGGCCC	1 cut(s)	1746
PspPI	GGNCC	7 cut(s)	228, 406, 708, 720, 1303, 1746, 1747
PstI	CTGCAG	1 cut(s)	262
PstNI	CAGNNNCTG	1 cut(s)	1595
PsuI	RGATCY	4 cut(s)	1393, 1708, 1810, 2205
PvuI	CGATCG	1 cut(s)	206
RsaI	GTAC	6 cut(s)	430, 964, 1233, 1733, 2000, 2323
RsaNI	GTAC	6 cut(s)	429, 963, 1232, 1732, 1999, 2322
RseI	CAYNNNNRTG	2 cut(s)	362, 1511
SacI	GAGCTC	1 cut(s)	1134
SaqAI	TTAA	7 cut(s)	102, 852, 1254, 1389, 1413, 2490, 2551
SatI	GCNGC	8 cut(s)	209, 261, 639, 668, 695, 924, 952, 2251
Sau96I	GGNCC	7 cut(s)	228, 406, 708, 720, 1303, 1746, 1747
SchI	GAGTC	2 cut(s)	1650, 2221
ScrFI	CCNGG	5 cut(s)	214, 240, 410, 426, 691
SduI	GDGCHC	5 cut(s)	10, 870, 1134, 1405, 1750
SfaNI	GCATC	1 cut(s)	1675
SfcI	CTRYAG	2 cut(s)	258, 2200
SinI	GGWCC	3 cut(s)	228, 406, 708
SmiMI	CAYNNNNRTG	2 cut(s)	362, 1511
SmlI	CTYRAG	2 cut(s)	11, 1253
SmoI	CTYRAG	2 cut(s)	11, 1253
SphI	GCATGC	1 cut(s)	1213
SseBI	AGGCCT	2 cut(s)	1674, 2238
SsiI	CCGC	7 cut(s)	60, 77, 180, 473, 697, 951, 2251
SspMI	CTAG	3 cut(s)	663, 1442, 2153
SstI	GAGCTC	1 cut(s)	1134
StuI	AGGCCT	2 cut(s)	1674, 2238
StyD4I	CCNGG	5 cut(s)	212, 238, 408, 424, 689
StyI	CCWWGG	1 cut(s)	85
TaaI	ACNGT	8 cut(s)	115, 235, 1790, 1985, 2003, 2086, 2126, 2399
TaiI	ACGT	3 cut(s)	133, 1237, 2519
TaqI	TCGA	4 cut(s)	309, 2032, 2217, 2349
TatI	WGTACW	1 cut(s)	962
TauI	GCSGC	2 cut(s)	954, 2253
TfiI	GAWTC	8 cut(s)	380, 413, 419, 508, 1355, 1615, 1912, 2329
Tru1I	TTAA	7 cut(s)	102, 852, 1254, 1389, 1413, 2490, 2551
Tru9I	TTAA	7 cut(s)	102, 852, 1254, 1389, 1413, 2490, 2551
TscAI	CASTG	5 cut(s)	270, 1153, 1960, 1990, 2089
TseI	GCWGC	6 cut(s)	208, 260, 638, 667, 694, 923
TspDTI	ATGAA	8 cut(s)	1019, 1470, 1825, 1904, 2011, 2115, 2279, 2295
TspGWI	ACGGA	4 cut(s)	1117, 1437, 1935, 2491
TspRI	CASTG	5 cut(s)	270, 1153, 1960, 1990, 2089
Van91I	CCANNNNNTGG	1 cut(s)	1595
Vha464I	CTTAAG	1 cut(s)	1253
VpaK11BI	GGWCC	3 cut(s)	228, 406, 708
XagI	CCTNNNNNAGG	2 cut(s)	715, 1484
XapI	RAATTY	7 cut(s)	515, 623, 767, 1087, 1959, 2011, 2544
XceI	RCATGY	3 cut(s)	1213, 2288, 2411
XcmI	CCANNNNNNNNNTGG	1 cut(s)	602
XmiI	GTMKAC	1 cut(s)	1945
XmnI	GAANNNNTTC	1 cut(s)	2286
XspI	CTAG	3 cut(s)	663, 1442, 2153

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.