Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0007519

Physical Location & Seq

Genomic Coordinates Forward (+)

38680425 .. 38686856

Gene Structure 6432 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G025680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGAGTGCCCTCAAGATCACCAAAAAGCACCACAAACACTTGAACAATCCTTTCCCTTCCGCACCCCTCTCTCTCCCGCTCATCCAAGGAAACCTTGTCTTTAATTCTCAAACGGTGCCTTCACCCCAACGTTTTGATATTGGAAAGGATTTTGAGCTGTCTTGGAACACCAGCAATGGCGGGTCTTTGTCAATTTCTCACCGATCGCAGCCCAGGAGAGCCATATGGTCCACCGTTCCTGGTCAAGCCTTTGTCTCTGCAGCAGTGGCTCAAACACGAGTGGAAGAAAGCAGGGGTTCTTTTGTGGTCGAAGACATTTGTGTTGATCTGGTTTGCCATCACCAAACCATTCAAGACATAAGGGTGATCAGTCATGATTCTCCATCTGGGTTTTCAGGGCTGGACCTGGAACCAGATTCTCCAGGTACCCAATTCCCTATTGTCCTTGTAACTGGCTGGGTTTTCAATACGGAGAGGAGGAAGAAGCGTTTTCTGAAATCTGGGAGTCCCAAATTTGAGGGGAAGGGATCTTCTGCTTGTGCAAAGTATTGGGTTTTGTTTGAACAGAAATGCAGTCATCAAATTGGGTTCAAAGTGAAGCTTGGGCAACCCAACTTTGAATTTCCCACCAAAGCAGCTACTTACAAGGGTTTTAGAACTAGGCTGCTGGGTCGGTTTCAGAAACCCAGGCTGCGGTGGTCTTGGTCCTTTACGAGGCCCAGAGGGTTTGTCTTGCTTCCCTCTGAGGCTCAACAATTCAAAGAATTCAACAGGGTGTGCCTGACATATTCAAGTGAAGAAAAGGAGAGGTTTTATGGATTTGGGGAGCAATTCTCTTATATGGATTTTAAGGGCAAAAGGGTGCCCATTTTTGTTCAGGAACAGGGTATTGGGAGAGGAGATCAGCCTATTACCTTTGCAGCTAACTTAGTCAGTTACAGGGCTGGCGGCGATTGGAGTACAACCTATGCTCCTTCCCCATTCTATATGACTTCGAAGATGAAATCTCTTTACCTTGAAGGATATGATTATTGCGTATTTGATCTAACACAACATGACAAAGTTCAGATAAAGATACATGGGAATTCAGTAGAGGGACGGATATTGCATGGAAACTCACCGACTGAGCTCATTGAATGTTTCACTGAAACCATTGGGAGACCTCCTAAACTTCCAGACTGGATTATTTCTGGAGCTGTTGTTGGCATGCAGGGTGGCACAAAATCTGTACGTCGTATTTGGAATGAGCTTAAGAGTTACAATGCTCCAGTTTCAGCATTCTGGTTGCAGGATTGGGTGGGCCACAGAGAGACATTGGTTGGATCACAACTCTGGTGGAATTGGGAAGTAGATTCAACAAGGTATATGGGATGGCAACAACTAATTAAAGATCTTAGTGCTCAGCATATTAAAGTGATGACATACTGCAACCCATGTCTAGCTCCGTGTCATGAAAAGCCAAACAGAAAGAGAAACCTCTTTGAGGAAGCAAAGAAGTTGGACATATTAGTGAAAGACAAGCAAGGAGAACCTTACATGGTTCCAAATACTGCTTTTGATGTAGGAATGTTGGACTTGACACATCCAGATACTGGAAATTGGTTCAAGCAGATTCTTCAGGAAATGGTTGATGATGGAGTCAGAGGATGGATGGCTGATTTTGGTGAAGGCCTGCCTGTGGATGCCACCCTCTATTCAGGCGAAGATCCTATTTCAGCCCACAACAAGTACCCAGAGCTATGGGCCCAAATAAATAGAGAGTTTGTGGAAGAATGGAAAGCTAACCGTGTGGGAAAGGAGAAAGAAGATCCACAAGAGGCGTTGGTCTTTTTCATGAGGGCTGGTTTCAGAGATAGCCCCAAATGGGGAATGCTATTTTGGGAAGGAGATCAGATGGTAAGTTGGCAGATTCATGACGGAATAAAGAGTGCTGTTGTTGGTCTACTCAGCAGTGGAATTTCAGGGTATGCTTTCAATCACAGTGATATTGGAGGGTACTGTGCTGTAAATTTACCTTTCATCAAGTATCGAAGAAGTGAAGAATTGCTTTTGCGATGGATGGAGCTAAATGCTTTCACCACTGTTTTCCGGACACATGAAGGAAACAAACCATCTTGCAACAGTCAATTCTACTCAAATGAGAGAACTCTAGCTCATTTTGCACGATTTGCTAAAGTCTATATAGCTTGGAAGTTCTATAGGATCCAGCTTGTCGAGGAAGCGACTCATAGAGGCCTGCCTGTATGCCGCCACTTATTTCTGCATTACCCTAATGATGAACATGTTCATAATTTGAGTTACCAGCAGTTCTTGATTGGTACTGAGATTCTGGTGGTGCCTGTCCTCGACAAAGGTATGAACAATGTGAAGGCTTATTTTCCAACTGGTAATGACTGTTGGCAACATGTATGGACTGGAAAGCAGTTTACTGAAGAGGGTTTTGAAACCATAGTAGAAGCTCAAATTGGTTACCCTGCTGTATTTGTTAAGACTGGTTCCGTTGTTGGAGAGACGTTTTTGAAAAACCTCAGAGATTTGAAAATTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_31

Protein Analysis

849

Amino Acids

97.17

Weight (kDa)

8.6

Isoelectric Point (pI)

44.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_31_2nd	PF01055	406 - 715	7.5e-37	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	745 - 835	3.4e-16	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007519)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360
malus_domestica	MD14G1090500.v1.1
prunus_persica	Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1
pyrus_communis	pycom14g08100
rosa_chinensis	RchiOBHm_Chr2g0125791
rosa_laevigata	RLG00000018880
rosa_multiflora	Rmu_sc0004466.1_g000001 Rmu_ssc0000055.1_g000006
rosa_roxburghii	Rroxscaffold_2G00117700
rosa_rugosa	Rorug02G0259300
rosa_samantha	Rh2AG317400 Rh2BG325500 Rh2CG307200 Rh2DG343400
rosa_wichuraiana	Rw2G025680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	425
AccB1I	GGYRCC	4 cut(s)	115, 425, 862, 2335
AccB7I	CCANNNNNTGG	1 cut(s)	1592
AccBSI	CCGCTC	1 cut(s)	79
AccI	GTMKAC	1 cut(s)	1942
AccIII	TCCGGA	1 cut(s)	2088
AciI	CCGC	6 cut(s)	60, 77, 180, 694, 948, 2248
AclI	AACGTT	1 cut(s)	130
AclWI	GGATC	6 cut(s)	535, 1330, 1700, 1802, 2197, 2210
AcsI	RAATTY	7 cut(s)	512, 620, 764, 1084, 1956, 2008, 2541
AcuI	CTGAAG	2 cut(s)	1601, 2451
AfaI	GTAC	6 cut(s)	427, 961, 1230, 1730, 1997, 2320
AfiI	CCNNNNNNNGG	7 cut(s)	693, 714, 1483, 1592, 1678, 1864, 1865
AflII	CTTAAG	1 cut(s)	1250
AflIII	ACRYGT	2 cut(s)	2281, 2404
AjnI	CCWGG	5 cut(s)	212, 238, 405, 421, 686
AjuI	GAANNNNNNNTTGG	6 cut(s)	873, 905, 1861, 1893, 2448, 2480
Alw21I	GWGCWC	2 cut(s)	1131, 1402
Alw26I	GTCTC	4 cut(s)	259, 1153, 1304, 2504
AlwI	GGATC	6 cut(s)	535, 1330, 1700, 1802, 2197, 2210
AlwNI	CAGNNNCTG	1 cut(s)	1592
Aor13HI	TCCGGA	1 cut(s)	2088
AoxI	GGCC	5 cut(s)	716, 1300, 1669, 1743, 2233
ApaI	GGGCCC	1 cut(s)	1747
ApeKI	GCWGC	6 cut(s)	208, 260, 635, 664, 691, 920
ApoI	RAATTY	7 cut(s)	512, 620, 764, 1084, 1956, 2008, 2541
Asp700I	GAANNNNTTC	1 cut(s)	2283
Asp718I	GGTACC	1 cut(s)	425
AspS9I	GGNCC	7 cut(s)	228, 403, 705, 717, 1300, 1743, 1744
AsuHPI	GGTGA	8 cut(s)	10, 114, 191, 332, 376, 1110, 1676, 2068
AsuII	TTCGAA	1 cut(s)	995
AvaII	GGWCC	3 cut(s)	228, 403, 705
BaeGI	GKGCMC	3 cut(s)	10, 867, 1747
BamHI	GGATCC	1 cut(s)	2202
BanI	GGYRCC	4 cut(s)	115, 425, 862, 2335
BanII	GRGCYC	2 cut(s)	1131, 1747
BauI	CACGAG	1 cut(s)	276
BbsI	GAAGAC	1 cut(s)	318
Bbv12I	GWGCWC	2 cut(s)	1131, 1402
BbvI	GCAGC	6 cut(s)	220, 272, 647, 651, 678, 932
BciT130I	CCWGG	5 cut(s)	214, 240, 407, 423, 688
BclI	TGATCA	1 cut(s)	366
BcoDI	GTCTC	4 cut(s)	259, 1153, 1304, 2504
BfaI	CTAG	3 cut(s)	660, 1439, 2150
BfmI	CTRYAG	2 cut(s)	258, 2197
BfrI	CTTAAG	1 cut(s)	1250
BglII	AGATCT	1 cut(s)	1390
BisI	GCNGC	8 cut(s)	209, 261, 636, 665, 692, 921, 949, 2248
BlpI	GCTNAGC	1 cut(s)	1401
BlsI	GCNGC	8 cut(s)	210, 262, 637, 666, 693, 922, 950, 2249
Bme1390I	CCNGG	5 cut(s)	214, 240, 407, 423, 688
Bme18I	GGWCC	3 cut(s)	228, 403, 705
BmgT120I	GGNCC	7 cut(s)	228, 403, 705, 717, 1300, 1743, 1744
BmiI	GGNNCC	9 cut(s)	117, 411, 427, 864, 1542, 1745, 2204, 2337, 2497
BmrFI	CCNGG	5 cut(s)	214, 240, 407, 423, 688
BmsI	GCATC	1 cut(s)	1672
BpiI	GAAGAC	1 cut(s)	318
BplI	GAGNNNNNCTC	2 cut(s)	818, 850
BpmI	CTGGAG	3 cut(s)	405, 1212, 1251
Bpu1102I	GCTNAGC	1 cut(s)	1401
Bpu14I	TTCGAA	1 cut(s)	995
BsaI	GGTCTC	1 cut(s)	1153
BsaJI	CCNNGG	3 cut(s)	85, 212, 686
BsaWI	WCCGGW	1 cut(s)	2088
BsaXI	ACNNNNNCTCC	4 cut(s)	955, 985, 1185, 1215
Bsc4I	CCNNNNNNNGG	7 cut(s)	693, 714, 1483, 1592, 1678, 1864, 1865
Bse1I	ACTGG	7 cut(s)	457, 1184, 1268, 1597, 2389, 2419, 2497
Bse3DI	GCAATG	1 cut(s)	181
BseAI	TCCGGA	1 cut(s)	2088
BseBI	CCWGG	5 cut(s)	214, 240, 407, 423, 688
BseDI	CCNNGG	3 cut(s)	85, 212, 686
BseGI	GGATG	7 cut(s)	81, 1376, 1582, 1652, 1656, 1687, 2064
BseLI	CCNNNNNNNGG	7 cut(s)	693, 714, 1483, 1592, 1678, 1864, 1865
BseMI	GCAATG	1 cut(s)	181
BseMII	CTCAG	6 cut(s)	735, 1116, 1415, 1960, 2313, 2542
BseNI	ACTGG	7 cut(s)	457, 1184, 1268, 1597, 2389, 2419, 2497
BseRI	GAGGAG	2 cut(s)	490, 912
BseSI	GKGCMC	3 cut(s)	10, 867, 1747
BseXI	GCAGC	6 cut(s)	220, 272, 647, 651, 678, 932
BseYI	CCCAGC	2 cut(s)	456, 667
Bsh1285I	CGRYCG	1 cut(s)	206
BshFI	GGCC	5 cut(s)	718, 1302, 1671, 1745, 2235
BshNI	GGYRCC	4 cut(s)	115, 425, 862, 2335
BsiEI	CGRYCG	1 cut(s)	206
BsiHKAI	GWGCWC	2 cut(s)	1131, 1402
BsiSI	CCGG	1 cut(s)	2089
BslFI	GGGAC	2 cut(s)	492, 1110
BslI	CCNNNNNNNGG	7 cut(s)	693, 714, 1483, 1592, 1678, 1864, 1865
BsmAI	GTCTC	4 cut(s)	259, 1153, 1304, 2504
BsmBI	CGTCTC	1 cut(s)	2504
BsmFI	GGGAC	2 cut(s)	492, 1110
BsmI	GAATGC	2 cut(s)	1277, 1875
BsnI	GGCC	5 cut(s)	718, 1302, 1671, 1745, 2235
Bso31I	GGTCTC	1 cut(s)	1153
Bsp119I	TTCGAA	1 cut(s)	995
Bsp120I	GGGCCC	1 cut(s)	1743
Bsp1286I	GDGCHC	5 cut(s)	10, 867, 1131, 1402, 1747
Bsp13I	TCCGGA	1 cut(s)	2088
Bsp1720I	GCTNAGC	1 cut(s)	1401
BspACI	CCGC	6 cut(s)	60, 77, 180, 694, 948, 2248
BspANI	GGCC	5 cut(s)	718, 1302, 1671, 1745, 2235
BspCNI	CTCAG	6 cut(s)	736, 1117, 1414, 1959, 2314, 2541
BspEI	TCCGGA	1 cut(s)	2088
BspHI	TCATGA	4 cut(s)	373, 1450, 1833, 1912
BspLI	GGNNCC	9 cut(s)	117, 411, 427, 864, 1542, 1745, 2204, 2337, 2497
BspMAI	CTGCAG	1 cut(s)	262
BspPI	GGATC	6 cut(s)	535, 1330, 1700, 1802, 2197, 2210
BspT104I	TTCGAA	1 cut(s)	995
BspT107I	GGYRCC	4 cut(s)	115, 425, 862, 2335
BspTI	CTTAAG	1 cut(s)	1250
BspTNI	GGTCTC	1 cut(s)	1153
BsrBI	CCGCTC	1 cut(s)	79
BsrDI	GCAATG	1 cut(s)	181
BsrI	ACTGG	7 cut(s)	457, 1184, 1268, 1597, 2389, 2419, 2497
BssECI	CCNNGG	3 cut(s)	85, 212, 686
BssSI	CACGAG	1 cut(s)	276
BssT1I	CCWWGG	1 cut(s)	85
Bst2BI	CACGAG	1 cut(s)	276
Bst2UI	CCWGG	5 cut(s)	214, 240, 407, 423, 688
Bst4CI	ACNGT	8 cut(s)	115, 235, 1787, 1982, 2000, 2083, 2123, 2396
Bst6I	CTCTTC	1 cut(s)	2427
BstAFI	CTTAAG	1 cut(s)	1250
BstAPI	GCANNNNNTGC	1 cut(s)	2168
BstBI	TTCGAA	1 cut(s)	995
BstC8I	GCNNGC	4 cut(s)	946, 1208, 1673, 2237
BstDEI	CTNAG	8 cut(s)	744, 928, 1125, 1394, 1401, 1946, 2322, 2528
BstEII	GGTNACC	1 cut(s)	2468
BstENI	CCTNNNNNAGG	2 cut(s)	712, 1481
BstF5I	GGATG	7 cut(s)	81, 1376, 1582, 1652, 1656, 1687, 2064
BstMAI	GTCTC	4 cut(s)	259, 1153, 1304, 2504
BstMCI	CGRYCG	1 cut(s)	206
BstMWI	GCNNNNNNNGC	3 cut(s)	266, 2159, 2168
BstNI	CCWGG	5 cut(s)	214, 240, 407, 423, 688
BstNSI	RCATGY	3 cut(s)	1210, 2285, 2408
BstPI	GGTNACC	1 cut(s)	2468
BstSCI	CCNGG	5 cut(s)	212, 238, 405, 421, 686
BstSFI	CTRYAG	2 cut(s)	258, 2197
BstSLI	GKGCMC	3 cut(s)	10, 867, 1747
BstV1I	GCAGC	6 cut(s)	220, 272, 647, 651, 678, 932
BstV2I	GAAGAC	1 cut(s)	318
BstX2I	RGATCY	5 cut(s)	527, 1390, 1705, 1807, 2202
BstXI	CCANNNNNNTGG	1 cut(s)	1740
BstYI	RGATCY	5 cut(s)	527, 1390, 1705, 1807, 2202
BsuRI	GGCC	5 cut(s)	718, 1302, 1671, 1745, 2235
BtgZI	GCGATG	1 cut(s)	2068
BtsCI	GGATG	7 cut(s)	81, 1376, 1582, 1652, 1656, 1687, 2064
BtsI	GCAGTG	2 cut(s)	270, 1957
BtsIMutI	CAGTG	5 cut(s)	270, 1143, 1957, 1987, 2079
Cac8I	GCNNGC	4 cut(s)	946, 1208, 1673, 2237
CaiI	CAGNNNCTG	1 cut(s)	1592
CciI	TCATGA	4 cut(s)	373, 1450, 1833, 1912
Cfr13I	GGNCC	7 cut(s)	228, 403, 705, 717, 1300, 1743, 1744
Csp6I	GTAC	6 cut(s)	426, 960, 1229, 1729, 1996, 2319
CspCI	CAANNNNNGTGG	2 cut(s)	1316, 1351
CviQI	GTAC	6 cut(s)	426, 960, 1229, 1729, 1996, 2319
DdeI	CTNAG	8 cut(s)	744, 928, 1125, 1394, 1401, 1946, 2322, 2528
Eam1104I	CTCTTC	1 cut(s)	2427
EarI	CTCTTC	1 cut(s)	2427
Ecl136II	GAGCTC	1 cut(s)	1129
Eco130I	CCWWGG	1 cut(s)	85
Eco147I	AGGCCT	2 cut(s)	1671, 2235
Eco24I	GRGCYC	2 cut(s)	1131, 1747
Eco31I	GGTCTC	1 cut(s)	1153
Eco47I	GGWCC	3 cut(s)	228, 403, 705
Eco53kI	GAGCTC	1 cut(s)	1129
Eco57I	CTGAAG	2 cut(s)	1601, 2451
Eco91I	GGTNACC	1 cut(s)	2468
EcoICRI	GAGCTC	1 cut(s)	1129
EcoNI	CCTNNNNNAGG	2 cut(s)	712, 1481
EcoO65I	GGTNACC	1 cut(s)	2468
EcoRI	GAATTC	2 cut(s)	764, 1084
EcoRII	CCWGG	5 cut(s)	212, 238, 405, 421, 686
EcoT14I	CCWWGG	1 cut(s)	85
EcoT38I	GRGCYC	2 cut(s)	1131, 1747
ErhI	CCWWGG	1 cut(s)	85
Esp3I	CGTCTC	1 cut(s)	2504
FalI	AAGNNNNNCTT	6 cut(s)	78, 110, 585, 617, 2031, 2063
FaqI	GGGAC	2 cut(s)	492, 1110
FauI	CCCGC	2 cut(s)	84, 173
FauNDI	CATATG	1 cut(s)	224
FbaI	TGATCA	1 cut(s)	366
FblI	GTMKAC	1 cut(s)	1942
Fnu4HI	GCNGC	8 cut(s)	209, 261, 636, 665, 692, 921, 949, 2248
FokI	GGATG	7 cut(s)	68, 1383, 1569, 1659, 1663, 1694, 2071
FriOI	GRGCYC	2 cut(s)	1131, 1747
Fsp4HI	GCNGC	8 cut(s)	209, 261, 636, 665, 692, 921, 949, 2248
FspBI	CTAG	3 cut(s)	660, 1439, 2150
GluI	GCNGC	8 cut(s)	209, 261, 636, 665, 692, 921, 949, 2248
GsaI	CCCAGC	2 cut(s)	460, 671
GsuI	CTGGAG	3 cut(s)	405, 1212, 1251
HaeIII	GGCC	5 cut(s)	718, 1302, 1671, 1745, 2235
HapII	CCGG	1 cut(s)	2089
HindIII	AAGCTT	1 cut(s)	599
HinfI	GANTC	9 cut(s)	377, 416, 505, 1352, 1612, 1638, 1909, 2224, 2326
HpaII	CCGG	1 cut(s)	2089
HphI	GGTGA	8 cut(s)	10, 114, 191, 332, 376, 1110, 1676, 2068
Hpy166II	GTNNAC	3 cut(s)	231, 1943, 2427
Hpy188I	TCNGA	8 cut(s)	495, 681, 745, 1068, 1643, 1850, 1893, 2531
Hpy8I	GTNNAC	3 cut(s)	231, 1943, 2427
Hpy99I	CGWCG	1 cut(s)	1236
HpyAV	CCTTC	9 cut(s)	66, 129, 517, 984, 1013, 1661, 1877, 2093, 2362
HpyCH4III	ACNGT	8 cut(s)	115, 235, 1787, 1982, 2000, 2083, 2123, 2396
HpyCH4IV	ACGT	3 cut(s)	130, 1231, 2513
HpyF10VI	GCNNNNNNNGC	3 cut(s)	266, 2159, 2168
HpyF3I	CTNAG	8 cut(s)	744, 928, 1125, 1394, 1401, 1946, 2322, 2528
HpySE526I	ACGT	3 cut(s)	130, 1231, 2513
Kpn2I	TCCGGA	1 cut(s)	2088
KpnI	GGTACC	1 cut(s)	429
Ksp22I	TGATCA	1 cut(s)	366
LmnI	GCTCC	6 cut(s)	826, 976, 1193, 1270, 1447, 2062
Lsp1109I	GCAGC	6 cut(s)	220, 272, 647, 651, 678, 932
LweI	GCATC	1 cut(s)	1672
MaeI	CTAG	3 cut(s)	660, 1439, 2150
MaeII	ACGT	3 cut(s)	130, 1231, 2513
MaeIII	GTNAC	5 cut(s)	448, 935, 1256, 2297, 2468
MbiI	CCGCTC	1 cut(s)	79
MflI	RGATCY	5 cut(s)	527, 1390, 1705, 1807, 2202
MhlI	GDGCHC	5 cut(s)	10, 867, 1131, 1402, 1747
MlyI	GAGTC	3 cut(s)	514, 1647, 2218
MmeI	TCCRAC	5 cut(s)	1300, 1479, 1551, 2405, 2485
MroI	TCCGGA	1 cut(s)	2088
MroXI	GAANNNNTTC	1 cut(s)	2283
MseI	TTAA	7 cut(s)	102, 849, 1251, 1386, 1410, 2487, 2548
MslI	CAYNNNNRTG	2 cut(s)	362, 1508
MspCI	CTTAAG	1 cut(s)	1250
MspI	CCGG	1 cut(s)	2089
MspR9I	CCNGG	5 cut(s)	214, 240, 407, 423, 688
Mva1269I	GAATGC	2 cut(s)	1277, 1875
MvaI	CCWGG	5 cut(s)	214, 240, 407, 423, 688
MwoI	GCNNNNNNNGC	3 cut(s)	266, 2159, 2168
NdeI	CATATG	1 cut(s)	224
NlaIV	GGNNCC	9 cut(s)	117, 411, 427, 864, 1542, 1745, 2204, 2337, 2497
NspI	RCATGY	3 cut(s)	1210, 2285, 2408
NspV	TTCGAA	1 cut(s)	995
PaeI	GCATGC	1 cut(s)	1210
PagI	TCATGA	4 cut(s)	373, 1450, 1833, 1912
PceI	AGGCCT	2 cut(s)	1671, 2235
PciI	ACATGT	2 cut(s)	2281, 2404
PctI	GAATGC	2 cut(s)	1277, 1875
PdmI	GAANNNNTTC	1 cut(s)	2283
PfeI	GAWTC	6 cut(s)	377, 416, 1352, 1612, 1909, 2326
PflMI	CCANNNNNTGG	1 cut(s)	1592
PkrI	GCNGC	8 cut(s)	210, 262, 637, 666, 693, 922, 950, 2249
Ple19I	CGATCG	1 cut(s)	206
PleI	GAGTC	3 cut(s)	513, 1646, 2218
PpsI	GAGTC	3 cut(s)	513, 1646, 2218
PscI	ACATGT	2 cut(s)	2281, 2404
Psp124BI	GAGCTC	1 cut(s)	1131
Psp1406I	AACGTT	1 cut(s)	130
Psp6I	CCWGG	5 cut(s)	212, 238, 405, 421, 686
PspEI	GGTNACC	1 cut(s)	2468
PspFI	CCCAGC	2 cut(s)	456, 667
PspGI	CCWGG	5 cut(s)	212, 238, 405, 421, 686
PspN4I	GGNNCC	9 cut(s)	117, 411, 427, 864, 1542, 1745, 2204, 2337, 2497
PspOMI	GGGCCC	1 cut(s)	1743
PspPI	GGNCC	7 cut(s)	228, 403, 705, 717, 1300, 1743, 1744
PstI	CTGCAG	1 cut(s)	262
PstNI	CAGNNNCTG	1 cut(s)	1592
PsuI	RGATCY	5 cut(s)	527, 1390, 1705, 1807, 2202
PvuI	CGATCG	1 cut(s)	206
RsaI	GTAC	6 cut(s)	427, 961, 1230, 1730, 1997, 2320
RsaNI	GTAC	6 cut(s)	426, 960, 1229, 1729, 1996, 2319
RseI	CAYNNNNRTG	2 cut(s)	362, 1508
SacI	GAGCTC	1 cut(s)	1131
SaqAI	TTAA	7 cut(s)	102, 849, 1251, 1386, 1410, 2487, 2548
SatI	GCNGC	8 cut(s)	209, 261, 636, 665, 692, 921, 949, 2248
Sau96I	GGNCC	7 cut(s)	228, 403, 705, 717, 1300, 1743, 1744
SchI	GAGTC	3 cut(s)	514, 1647, 2218
ScrFI	CCNGG	5 cut(s)	214, 240, 407, 423, 688
SduI	GDGCHC	5 cut(s)	10, 867, 1131, 1402, 1747
SfaNI	GCATC	1 cut(s)	1672
SfcI	CTRYAG	2 cut(s)	258, 2197
SfuI	TTCGAA	1 cut(s)	995
SinI	GGWCC	3 cut(s)	228, 403, 705
SmiMI	CAYNNNNRTG	2 cut(s)	362, 1508
SmlI	CTYRAG	2 cut(s)	11, 1250
SmoI	CTYRAG	2 cut(s)	11, 1250
SphI	GCATGC	1 cut(s)	1210
SseBI	AGGCCT	2 cut(s)	1671, 2235
SsiI	CCGC	6 cut(s)	60, 77, 180, 694, 948, 2248
SspMI	CTAG	3 cut(s)	660, 1439, 2150
SstI	GAGCTC	1 cut(s)	1131
StuI	AGGCCT	2 cut(s)	1671, 2235
StyD4I	CCNGG	5 cut(s)	212, 238, 405, 421, 686
StyI	CCWWGG	1 cut(s)	85
TaaI	ACNGT	8 cut(s)	115, 235, 1787, 1982, 2000, 2083, 2123, 2396
TaiI	ACGT	3 cut(s)	133, 1234, 2516
TaqI	TCGA	5 cut(s)	309, 995, 2029, 2214, 2346
TatI	WGTACW	1 cut(s)	959
TauI	GCSGC	2 cut(s)	951, 2250
TfiI	GAWTC	6 cut(s)	377, 416, 1352, 1612, 1909, 2326
Tru1I	TTAA	7 cut(s)	102, 849, 1251, 1386, 1410, 2487, 2548
Tru9I	TTAA	7 cut(s)	102, 849, 1251, 1386, 1410, 2487, 2548
TscAI	CASTG	5 cut(s)	270, 1150, 1957, 1987, 2086
TseI	GCWGC	6 cut(s)	208, 260, 635, 664, 691, 920
TspDTI	ATGAA	9 cut(s)	1016, 1467, 1822, 1901, 2008, 2112, 2276, 2292, 2372
TspGWI	ACGGA	5 cut(s)	485, 1114, 1434, 1932, 2488
TspRI	CASTG	5 cut(s)	270, 1150, 1957, 1987, 2086
Van91I	CCANNNNNTGG	1 cut(s)	1592
Vha464I	CTTAAG	1 cut(s)	1250
VpaK11BI	GGWCC	3 cut(s)	228, 403, 705
XagI	CCTNNNNNAGG	2 cut(s)	712, 1481
XapI	RAATTY	7 cut(s)	512, 620, 764, 1084, 1956, 2008, 2541
XceI	RCATGY	3 cut(s)	1210, 2285, 2408
XmiI	GTMKAC	1 cut(s)	1942
XmnI	GAANNNNTTC	1 cut(s)	2283
XspI	CTAG	3 cut(s)	660, 1439, 2150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.