MD14G1117300.v1.1

Acyl-coenzyme A:6-aminopenicillanic acid acyl-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0018099
Physical Location & Seq
Reverse (-)
19144332 .. 19146572
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1117300.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGGGAGCTCTGCAAAACTAGGGTTGGAGATGTTTGAAGTTGGACCCTGTGAAGATGCTTACACAATGGGATTCCTAATTGGCCAGAGATTTTCAGACATGATAAAGAGCAGGCTGGCCTCAGACCTCATTCTTCACAACCAACTCCTCCCCTTTGCTCAAACCCCAGAGTTTCAGCCACTTCTCCAGTCTCTAACAGAAAATAACCGAAACAAGTTTCCGAGATATTGGGAGGAGCTCGTTGGCACTGCGGATGGAAGTGGAGTTCCAGTTCTTGATATAATACTAATCAACTTCAGAAAGGAGATTCTTCCGCTTCTCCCAAAGACCACGGCGATGAATCAGAATATTGATACGCTGGATGACTGCTCGGATGTTCTTGTTGTTAGTGATTCCATGGCAGTTGTGGCACACAATGAGGATGCAAATGTTGCCCTGGTTGGCCACACCTACTTGATAAAGGGGACTCTGTCAAATGGGATATCATTCATGGCTTACACTTATGCAGGAGAGCTCCCTAGCTGTGCATTTGGGCTCAACACTCATGGACTGGCTTTTACGCTGAATTCAGTTCCCCCGTCGGAAAATGAGATTGTTGCCGGAGGCATTGGGAGGAACTTTGTTTCACGGGACCTCCTTGAAGCATCAAGCATCGACGACGCATTATGTAGAATTAAATCATCAGAAGTATCTGTTGGACATAGTTACAATTTGATCGAGACGAGGACACGCAAAATTATGAACGTAGAAACTGCTTCAAGAAAACGGGTTTCGATTTATGAGGTTGGAGCAACACCATTCTTCCATGCAAACATGTATCTCCATCTCCAAGTTAATCAGTTGCTAGATGAAAACTCAATAGCAAGGCAAAGCAGAGCAGCTGCGCTACGTAAAAGATCGAAAGACAATTTCCTATCTCTCCTAGGAGATACGGATGACCCGAAATATCCAATCTACATGATAGGTCCATTGCTTTATACACTATGCACGGCCGTGATAGACTTGGACGAACAAACACTGTCGATAGTTGAAGGGAACCCGAGAAAAGGAAAAATCTCTCACATCTTCTCTACGTGCACGGAAGATTTCAAGATGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAT

Protein Analysis

367

Amino Acids

40.44

Weight (kDa)

5.24

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAT PF03417 132 - 348 2e-26 Acyl-coenzyme A:6-aminopenicillanic acid acyl-transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018099)

Species Orthologous Gene IDs
malus_domestica MD14G1117300.v1.1
prunus_persica Prupe.5G112000_v2.0.a1 Prupe.5G112000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0195891
rosa_laevigata RLG00000004082
rosa_multiflora Rmu_sc0004016.1_g000008
rosa_roxburghii Rroxscaffold_3G00260020
rosa_rugosa Rorug07G0027100
rosa_samantha Rh7AG154800 Rh7CG160300
rosa_wichuraiana Rw7G013230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 251, 314
AcoI YGGCCR 3 cut(s) 82, 442, 990
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 280
AfiI CCNNNNNNNGG 1 cut(s) 1046
AflIII ACRYGT 1 cut(s) 813
AgsI TTSAA 5 cut(s) 38, 641, 759, 1031, 1090
AjnI CCWGG 1 cut(s) 435
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
AleI CACNNNNGTG 1 cut(s) 992
AloI GAACNNNNNNTCC 4 cut(s) 249, 255, 281, 287
AluBI AGCT 5 cut(s) 9, 238, 514, 522, 881
AluI AGCT 5 cut(s) 9, 238, 514, 522, 881
Alw21I GWGCWC 4 cut(s) 11, 240, 516, 1079
Alw26I GTCTC 2 cut(s) 195, 713
Alw44I GTGCAC 1 cut(s) 1075
Ama87I CYCGRG 1 cut(s) 1039
AoxI GGCC 4 cut(s) 82, 117, 442, 990
ApaLI GTGCAC 1 cut(s) 1075
ApeKI GCWGC 2 cut(s) 878, 881
ApoI RAATTY 1 cut(s) 565
AspA2I CCTAGG 1 cut(s) 922
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 3 cut(s) 44, 631, 965
AvaI CYCGRG 1 cut(s) 1039
AvaII GGWCC 3 cut(s) 44, 631, 965
AvrII CCTAGG 1 cut(s) 922
BaeGI GKGCMC 1 cut(s) 1079
BalI TGGCCA 2 cut(s) 84, 444
BanII GRGCYC 4 cut(s) 11, 240, 516, 537
Bbv12I GWGCWC 4 cut(s) 11, 240, 516, 1079
BbvI GCAGC 2 cut(s) 868, 890
BccI CCATC 2 cut(s) 248, 831
BceAI ACGGC 3 cut(s) 348, 977, 1005
BciT130I CCWGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 195, 713
BfaI CTAG 4 cut(s) 20, 519, 845, 923
BisI GCNGC 2 cut(s) 879, 882
BlnI CCTAGG 1 cut(s) 922
BlsI GCNGC 2 cut(s) 880, 883
Bme1390I CCNGG 1 cut(s) 437
Bme18I GGWCC 3 cut(s) 44, 631, 965
BmeT110I CYCGRG 1 cut(s) 1039
BmgT120I GGNCC 3 cut(s) 44, 631, 965
BmiI GGNNCC 3 cut(s) 46, 632, 1037
BmrFI CCNGG 1 cut(s) 437
BmsI GCATC 5 cut(s) 46, 412, 653, 660, 1083
BpmI CTGGAG 1 cut(s) 170
BsaAI YACGTR 2 cut(s) 890, 1074
BsaJI CCNNGG 4 cut(s) 330, 396, 435, 922
BsaXI ACNNNNNCTCC 4 cut(s) 255, 285, 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 1046
Bse1I ACTGG 3 cut(s) 187, 269, 555
Bse3DI GCAATG 1 cut(s) 968
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 4 cut(s) 330, 396, 435, 922
BseGI GGATG 5 cut(s) 259, 367, 379, 427, 940
BseLI CCNNNNNNNGG 1 cut(s) 1046
BseMI GCAATG 1 cut(s) 968
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 3 cut(s) 187, 269, 555
BseRI GAGGAG 2 cut(s) 137, 248
BseSI GKGCMC 1 cut(s) 1079
BseX3I CGGCCG 1 cut(s) 990
BseXI GCAGC 2 cut(s) 868, 890
Bsh1285I CGRYCG 1 cut(s) 993
BshFI GGCC 4 cut(s) 84, 119, 444, 992
BsiEI CGRYCG 1 cut(s) 993
BsiHKAI GWGCWC 4 cut(s) 11, 240, 516, 1079
BsiHKCI CYCGRG 1 cut(s) 1039
BsiSI CCGG 1 cut(s) 600
BslFI GGGAC 2 cut(s) 478, 644
BslI CCNNNNNNNGG 1 cut(s) 1046
BsmAI GTCTC 2 cut(s) 195, 713
BsmBI CGTCTC 1 cut(s) 713
BsmFI GGGAC 2 cut(s) 478, 644
BsnI GGCC 4 cut(s) 84, 119, 444, 992
BsoBI CYCGRG 1 cut(s) 1039
Bsp1286I GDGCHC 5 cut(s) 11, 240, 516, 537, 1079
Bsp143I GATC 2 cut(s) 714, 896
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 2 cut(s) 251, 314
BspANI GGCC 4 cut(s) 84, 119, 444, 992
BspCNI CTCAG 1 cut(s) 134
BspLI GGNNCC 3 cut(s) 46, 632, 1037
BsrDI GCAATG 1 cut(s) 968
BsrI ACTGG 3 cut(s) 187, 269, 555
BssECI CCNNGG 4 cut(s) 330, 396, 435, 922
BssMI GATC 2 cut(s) 714, 896
BssT1I CCWWGG 2 cut(s) 396, 922
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 1020
BstAPI GCANNNNNTGC 1 cut(s) 431
BstBAI YACGTR 2 cut(s) 890, 1074
BstC8I GCNNGC 2 cut(s) 113, 117
BstDEI CTNAG 1 cut(s) 121
BstDSI CCRYGG 2 cut(s) 330, 396
BstF5I GGATG 5 cut(s) 259, 367, 379, 427, 940
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 2 cut(s) 717, 899
BstMAI GTCTC 2 cut(s) 195, 713
BstMBI GATC 2 cut(s) 714, 896
BstMCI CGRYCG 1 cut(s) 993
BstMWI GCNNNNNNNGC 2 cut(s) 407, 431
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 1079
BstSNI TACGTA 1 cut(s) 890
BstV1I GCAGC 2 cut(s) 868, 890
BstZI CGGCCG 1 cut(s) 990
BsuRI GGCC 4 cut(s) 84, 119, 444, 992
BtgI CCRYGG 2 cut(s) 330, 396
BtgZI GCGATG 1 cut(s) 350
BtsCI GGATG 5 cut(s) 259, 367, 379, 427, 940
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 246, 1016
Cac8I GCNNGC 2 cut(s) 113, 117
CfoI GCGC 1 cut(s) 886
Cfr13I GGNCC 3 cut(s) 44, 631, 965
CseI GACGC 1 cut(s) 668
CviAII CATG 7 cut(s) 100, 397, 490, 545, 806, 814, 958
DdeI CTNAG 1 cut(s) 121
DpnI GATC 2 cut(s) 716, 898
DpnII GATC 2 cut(s) 714, 896
EaeI YGGCCR 3 cut(s) 82, 442, 990
EagI CGGCCG 1 cut(s) 990
Ecl136II GAGCTC 3 cut(s) 9, 238, 514
EclXI CGGCCG 1 cut(s) 990
Eco105I TACGTA 1 cut(s) 890
Eco130I CCWWGG 2 cut(s) 396, 922
Eco24I GRGCYC 4 cut(s) 11, 240, 516, 537
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 3 cut(s) 44, 631, 965
Eco52I CGGCCG 1 cut(s) 990
Eco53kI GAGCTC 3 cut(s) 9, 238, 514
Eco57I CTGAAG 1 cut(s) 280
Eco88I CYCGRG 1 cut(s) 1039
EcoICRI GAGCTC 3 cut(s) 9, 238, 514
EcoO109I RGGNCCY 1 cut(s) 631
EcoRI GAATTC 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 435
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 2 cut(s) 396, 922
EcoT38I GRGCYC 4 cut(s) 11, 240, 516, 537
ErhI CCWWGG 2 cut(s) 396, 922
Esp3I CGTCTC 1 cut(s) 713
FaeI CATG 7 cut(s) 103, 400, 493, 548, 809, 817, 961
FaqI GGGAC 2 cut(s) 478, 644
FatI CATG 7 cut(s) 99, 396, 489, 544, 805, 813, 957
Fnu4HI GCNGC 2 cut(s) 879, 882
FokI GGATG 5 cut(s) 266, 374, 386, 434, 947
FriOI GRGCYC 4 cut(s) 11, 240, 516, 537
Fsp4HI GCNGC 2 cut(s) 879, 882
FspBI CTAG 4 cut(s) 20, 519, 845, 923
GlaI GCGC 1 cut(s) 885
GluI GCNGC 2 cut(s) 879, 882
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 4 cut(s) 84, 119, 444, 992
HapII CCGG 1 cut(s) 600
HgaI GACGC 1 cut(s) 668
HhaI GCGC 1 cut(s) 886
Hin1II CATG 7 cut(s) 103, 400, 493, 548, 809, 817, 961
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HinfI GANTC 5 cut(s) 72, 307, 340, 392, 466
HpaII CCGG 1 cut(s) 600
Hpy166II GTNNAC 1 cut(s) 1077
Hpy188I TCNGA 8 cut(s) 97, 124, 222, 299, 345, 373, 583, 685
Hpy188III TCNNGA 4 cut(s) 275, 718, 759, 1090
Hpy8I GTNNAC 1 cut(s) 1077
Hpy99I CGWCG 3 cut(s) 583, 659, 662
HpyAV CCTTC 1 cut(s) 1025
HpyCH4III ACNGT 1 cut(s) 1020
HpyCH4IV ACGT 3 cut(s) 744, 889, 1073
HpyCH4V TGCA 7 cut(s) 14, 425, 506, 527, 809, 987, 1077
HpyF10VI GCNNNNNNNGC 2 cut(s) 407, 431
HpyF3I CTNAG 1 cut(s) 121
HpySE526I ACGT 3 cut(s) 744, 889, 1073
Hsp92II CATG 7 cut(s) 103, 400, 493, 548, 809, 817, 961
HspAI GCGC 1 cut(s) 884
Kzo9I GATC 2 cut(s) 714, 896
LmnI GCTCC 4 cut(s) 6, 235, 519, 788
Lsp1109I GCAGC 2 cut(s) 868, 890
LweI GCATC 5 cut(s) 46, 412, 653, 660, 1083
MaeI CTAG 4 cut(s) 20, 519, 845, 923
MaeII ACGT 3 cut(s) 744, 889, 1073
MaeIII GTNAC 1 cut(s) 704
MalI GATC 2 cut(s) 716, 898
MboI GATC 2 cut(s) 714, 896
MboII GAAGA 6 cut(s) 65, 125, 302, 793, 1057, 1094
MhlI GDGCHC 5 cut(s) 11, 240, 516, 537, 1079
MlsI TGGCCA 2 cut(s) 84, 444
MluCI AATT 6 cut(s) 78, 565, 672, 709, 735, 907
MluNI TGGCCA 2 cut(s) 84, 444
MlyI GAGTC 1 cut(s) 460
MmeI TCCRAC 5 cut(s) 6, 22, 561, 676, 766
Mox20I TGGCCA 2 cut(s) 84, 444
MscI TGGCCA 2 cut(s) 84, 444
MseI TTAA 2 cut(s) 675, 834
MslI CAYNNNNRTG 2 cut(s) 335, 992
Msp20I TGGCCA 2 cut(s) 84, 444
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 1 cut(s) 600
MspR9I CCNGG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 437
MwoI GCNNNNNNNGC 2 cut(s) 407, 431
NcoI CCATGG 1 cut(s) 396
NdeII GATC 2 cut(s) 714, 896
NlaIII CATG 7 cut(s) 103, 400, 493, 548, 809, 817, 961
NlaIV GGNNCC 3 cut(s) 46, 632, 1037
NspI RCATGY 1 cut(s) 817
OliI CACNNNNGTG 1 cut(s) 992
PciI ACATGT 1 cut(s) 813
PcsI WCGNNNNNNNCGW 1 cut(s) 938
PfeI GAWTC 4 cut(s) 72, 307, 340, 392
PflFI GACNNNGTC 1 cut(s) 469
PkrI GCNGC 2 cut(s) 880, 883
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
Ppu21I YACGTR 2 cut(s) 890, 1074
PpuMI RGGWCCY 1 cut(s) 631
PscI ACATGT 1 cut(s) 813
Psp124BI GAGCTC 3 cut(s) 11, 240, 516
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 3 cut(s) 46, 632, 1037
PspPI GGNCC 3 cut(s) 44, 631, 965
PspPPI RGGWCCY 1 cut(s) 631
PsyI GACNNNGTC 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 881
RseI CAYNNNNRTG 2 cut(s) 335, 992
SacI GAGCTC 3 cut(s) 11, 240, 516
SaqAI TTAA 2 cut(s) 675, 834
SatI GCNGC 2 cut(s) 879, 882
Sau3AI GATC 2 cut(s) 714, 896
Sau96I GGNCC 3 cut(s) 44, 631, 965
SchI GAGTC 1 cut(s) 460
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 5 cut(s) 11, 240, 516, 537, 1079
SfaNI GCATC 5 cut(s) 46, 412, 653, 660, 1083
SinI GGWCC 3 cut(s) 44, 631, 965
SmiMI CAYNNNNRTG 2 cut(s) 335, 992
SnaBI TACGTA 1 cut(s) 890
Sse9I AATT 6 cut(s) 78, 565, 672, 709, 735, 907
SsiI CCGC 2 cut(s) 251, 314
SspI AATATT 1 cut(s) 349
SspMI CTAG 4 cut(s) 20, 519, 845, 923
SstI GAGCTC 3 cut(s) 11, 240, 516
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 2 cut(s) 396, 922
TaaI ACNGT 1 cut(s) 1020
TaiI ACGT 3 cut(s) 747, 892, 1076
TaqI TCGA 5 cut(s) 654, 717, 773, 899, 1022
TasI AATT 6 cut(s) 78, 565, 672, 709, 735, 907
TfiI GAWTC 4 cut(s) 72, 307, 340, 392
Tru1I TTAA 2 cut(s) 675, 834
Tru9I TTAA 2 cut(s) 675, 834
TscAI CASTG 2 cut(s) 253, 1023
TseI GCWGC 2 cut(s) 878, 881
TspDTI ATGAA 4 cut(s) 353, 478, 755, 864
TspGWI ACGGA 2 cut(s) 947, 1094
TspRI CASTG 2 cut(s) 253, 1023
Tth111I GACNNNGTC 1 cut(s) 469
VneI GTGCAC 1 cut(s) 1075
VpaK11BI GGWCC 3 cut(s) 44, 631, 965
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 817
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmaJI CCTAGG 1 cut(s) 922
XspI CTAG 4 cut(s) 20, 519, 845, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.