RLG00000004082

Acyl-coenzyme A:6-aminopenicillanic acid acyl-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018099
Physical Location & Seq
Reverse (-)
55966004 .. 55968016
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004082

Sequence Viewer

Length: 1104 bp
ATGGAGAGCTCTAGCATGGAAGCAGAAGCTTTGGAGATATTTGAGGTTGGTCCTTGTGAAGATGCTCGCACAATGGGCTACATGATAGGCCAGAGGTTTTCAAACAAGATAAAGAGCAGGTTGGCCAGAGACCTCATTCTTCAAGACAAGCTCCTCCCTTTTGCTCAGACCCCAGAGTCACAGCCACTCATTCAGTCTCTTACTGAAAATCACCGGAGCAAGTTTCCGAGGTATTGGGACGAGCTCGTAGGAACTGCAGAGGGAAGTGGTGTGCCAGTTCTTGAAATAATGCTGATCAACTTTAGGAAGGAGATTCTTCCATTTCTTCCAAAGACGGTGGTGAATTGCGAAATAGATACCCCGGATAACTGCTCTGATGTTCTTGTTGCTAGTGATTCCATGGCAATTGTGGCTCACAATGAGGATGCAAATGTTGCTCTGGTTGGCCATACCTATTTGGTAAAGGGAACTCTGTTAAATGGGATATCATTCATTGCTTATACATATGCCGGAGAGCTCCCTAGCTGTGCGTTCGGCGTCAACAGTCATGGCCTGGCATTTACGTTGAATTCGGTACCCCCATCTGAAAGTGAGATTGTTCCTGGAGGCATTGGGCGGAACTTTATTTCACGAGACCTGCTTGAAGCAAAGAGCATTATTGATGCATTATGTAGAATTGAATCAGCAGAAGTTTCTGCAGGACATAGTTACAATCTGATTGAGACAAGGACACGAAGAATTATCAATGTAGAAACTGCATCAAAGAACCGGGTTTCAGTTTTTGAGGTTGAAGAAACACCATTCTTCCATGCAAACATGTATCTGCATCTCCAGGTTAATCAGGTACATGATGAAAATTCATTAACAAGGCAAAGGAAAGCAACTATGCTACCTAAAGGCTCCAAAGAAGATGTCCTGTCACTTCTAGGAGATACAGACGAGCCAACATACCCCATCTACATGACAGGTCCAACGCTTTACACTCTATGCACAGCAGTGATAGACCTCGACGAACAAACCCTATCAATTGTTGAAGGAAACCCGAAAAAAGGAGAAATCTCTCACATCTTCTCTACATGTTCAGAAGATTTCAAGATGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAT

Protein Analysis

368

Amino Acids

40.63

Weight (kDa)

4.9

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAT PF03417 132 - 349 6.3e-26 Acyl-coenzyme A:6-aminopenicillanic acid acyl-transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018099)

Species Orthologous Gene IDs
malus_domestica MD14G1117300.v1.1
prunus_persica Prupe.5G112000_v2.0.a1 Prupe.5G112000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0195891
rosa_laevigata RLG00000004082
rosa_multiflora Rmu_sc0004016.1_g000008
rosa_roxburghii Rroxscaffold_3G00260020
rosa_rugosa Rorug07G0027100
rosa_samantha Rh7AG154800 Rh7CG160300
rosa_wichuraiana Rw7G013230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 175
Acc36I ACCTGC 2 cut(s) 108, 645
Acc65I GGTACC 1 cut(s) 574
AccB1I GGYRCC 1 cut(s) 574
AciI CCGC 1 cut(s) 616
AcoI YGGCCR 2 cut(s) 123, 445
AcsI RAATTY 2 cut(s) 568, 856
AcyI GRCGYC 1 cut(s) 537
AfaI GTAC 2 cut(s) 576, 846
AfiI CCNNNNNNNGG 1 cut(s) 1049
AflIII ACRYGT 2 cut(s) 816, 1076
AgsI TTSAA 9 cut(s) 102, 143, 284, 568, 644, 680, 791, 1034, 1093
AjnI CCWGG 3 cut(s) 552, 601, 831
AleI CACNNNNGTG 1 cut(s) 995
AluBI AGCT 6 cut(s) 9, 29, 151, 244, 517, 525
AluI AGCT 6 cut(s) 9, 29, 151, 244, 517, 525
Alw21I GWGCWC 3 cut(s) 11, 246, 519
Alw26I GTCTC 4 cut(s) 123, 201, 627, 716
AoxI GGCC 4 cut(s) 88, 123, 445, 550
ApoI RAATTY 2 cut(s) 568, 856
ArsI GACNNNNNNTTYG 2 cut(s) 897, 929
Asp718I GGTACC 1 cut(s) 574
AspS9I GGNCC 2 cut(s) 50, 968
AsuC2I CCSGG 2 cut(s) 362, 770
AsuHPI GGTGA 2 cut(s) 203, 352
AvaII GGWCC 2 cut(s) 50, 968
BalI TGGCCA 2 cut(s) 125, 447
BanI GGYRCC 1 cut(s) 574
BanII GRGCYC 3 cut(s) 11, 246, 519
BauI CACGAG 1 cut(s) 630
Bbv12I GWGCWC 3 cut(s) 11, 246, 519
BccI CCATC 2 cut(s) 589, 962
BciT130I CCWGG 3 cut(s) 554, 603, 833
BclI TGATCA 1 cut(s) 294
BcnI CCSGG 2 cut(s) 362, 770
BcoDI GTCTC 4 cut(s) 123, 201, 627, 716
BfaI CTAG 4 cut(s) 12, 390, 522, 926
BfmI CTRYAG 2 cut(s) 255, 696
BfuAI ACCTGC 2 cut(s) 108, 645
Bme1390I CCNGG 5 cut(s) 362, 554, 603, 770, 833
Bme18I GGWCC 2 cut(s) 50, 968
BmgT120I GGNCC 2 cut(s) 50, 968
BmiI GGNNCC 2 cut(s) 576, 901
BmrFI CCNGG 5 cut(s) 362, 554, 603, 770, 833
BmsI GCATC 6 cut(s) 52, 415, 652, 767, 835, 1086
BpmI CTGGAG 2 cut(s) 624, 815
BpuMI CCSGG 2 cut(s) 362, 770
BsaHI GRCGYC 1 cut(s) 537
BsaI GGTCTC 2 cut(s) 123, 627
BsaJI CCNNGG 3 cut(s) 227, 360, 399
BsaWI WCCGGW 1 cut(s) 213
Bsc4I CCNNNNNNNGG 1 cut(s) 1049
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 492
BseBI CCWGG 3 cut(s) 554, 603, 833
BseDI CCNNGG 3 cut(s) 227, 360, 399
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 1 cut(s) 1049
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 1 cut(s) 179
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 143
BshFI GGCC 4 cut(s) 90, 125, 447, 552
BshNI GGYRCC 1 cut(s) 574
BsiHKAI GWGCWC 3 cut(s) 11, 246, 519
BsiSI CCGG 4 cut(s) 214, 362, 510, 769
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 1049
BsmAI GTCTC 4 cut(s) 123, 201, 627, 716
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 4 cut(s) 90, 125, 447, 552
Bso31I GGTCTC 2 cut(s) 123, 627
Bsp1286I GDGCHC 3 cut(s) 11, 246, 519
Bsp143I GATC 1 cut(s) 294
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 1 cut(s) 616
BspANI GGCC 4 cut(s) 90, 125, 447, 552
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 2 cut(s) 576, 901
BspMAI CTGCAG 2 cut(s) 259, 700
BspMI ACCTGC 2 cut(s) 108, 645
BspT107I GGYRCC 1 cut(s) 574
BspTNI GGTCTC 2 cut(s) 123, 627
BsrDI GCAATG 1 cut(s) 492
BsrI ACTGG 1 cut(s) 275
BssECI CCNNGG 3 cut(s) 227, 360, 399
BssMI GATC 1 cut(s) 294
BssNI GRCGYC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 3 cut(s) 554, 603, 833
Bst4CI ACNGT 2 cut(s) 337, 545
BstACI GRCGYC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 1 cut(s) 165
BstDSI CCRYGG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 430
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 4 cut(s) 123, 201, 627, 716
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 3 cut(s) 75, 410, 434
BstNI CCWGG 3 cut(s) 554, 603, 833
BstNSI RCATGY 2 cut(s) 820, 1080
BstSCI CCNGG 5 cut(s) 360, 552, 601, 768, 831
BstSFI CTRYAG 2 cut(s) 255, 696
BsuRI GGCC 4 cut(s) 90, 125, 447, 552
BtgI CCRYGG 1 cut(s) 399
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 1002
BtsIMutI CAGTG 1 cut(s) 1002
BveI ACCTGC 2 cut(s) 108, 645
Cac8I GCNNGC 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 50, 968
CseI GACGC 1 cut(s) 526
Csp6I GTAC 2 cut(s) 575, 845
CspCI CAANNNNNGTGG 2 cut(s) 318, 353
CviAII CATG 9 cut(s) 16, 82, 400, 548, 809, 817, 848, 961, 1077
CviQI GTAC 2 cut(s) 575, 845
DdeI CTNAG 1 cut(s) 165
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
DrdI GACNNNNNNGTC 1 cut(s) 175
DseDI GACNNNNNNGTC 1 cut(s) 175
EaeI YGGCCR 2 cut(s) 123, 445
EciI GGCGGA 1 cut(s) 631
Ecl136II GAGCTC 3 cut(s) 9, 244, 517
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 3 cut(s) 11, 246, 519
Eco31I GGTCTC 2 cut(s) 123, 627
Eco32I GATATC 1 cut(s) 486
Eco47I GGWCC 2 cut(s) 50, 968
Eco53kI GAGCTC 3 cut(s) 9, 244, 517
EcoICRI GAGCTC 3 cut(s) 9, 244, 517
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 3 cut(s) 552, 601, 831
EcoRV GATATC 1 cut(s) 486
EcoT14I CCWWGG 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 667
EcoT38I GRGCYC 3 cut(s) 11, 246, 519
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 9 cut(s) 19, 85, 403, 551, 812, 820, 851, 964, 1080
FaqI GGGAC 1 cut(s) 251
FatI CATG 9 cut(s) 15, 81, 399, 547, 808, 816, 847, 960, 1076
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 294
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 3 cut(s) 11, 246, 519
FspBI CTAG 4 cut(s) 12, 390, 522, 926
GsuI CTGGAG 2 cut(s) 624, 815
HaeIII GGCC 4 cut(s) 90, 125, 447, 552
HapII CCGG 4 cut(s) 214, 362, 510, 769
HgaI GACGC 1 cut(s) 526
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 9 cut(s) 19, 85, 403, 551, 812, 820, 851, 964, 1080
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 27
HinfI GANTC 4 cut(s) 176, 313, 395, 680
HpaII CCGG 4 cut(s) 214, 362, 510, 769
HphI GGTGA 2 cut(s) 203, 352
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 6 cut(s) 168, 228, 376, 586, 717, 1084
Hpy188III TCNNGA 4 cut(s) 143, 281, 630, 1093
Hpy8I GTNNAC 1 cut(s) 541
Hpy99I CGWCG 1 cut(s) 1013
HpyAV CCTTC 2 cut(s) 301, 1028
HpyCH4III ACNGT 2 cut(s) 337, 545
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 8 cut(s) 257, 428, 665, 698, 758, 812, 826, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 410, 434
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 563
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 9 cut(s) 19, 85, 403, 551, 812, 820, 851, 964, 1080
KpnI GGTACC 1 cut(s) 578
Ksp22I TGATCA 1 cut(s) 294
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 4 cut(s) 156, 216, 522, 905
LweI GCATC 6 cut(s) 52, 415, 652, 767, 835, 1086
MaeI CTAG 4 cut(s) 12, 390, 522, 926
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 3 cut(s) 177, 707, 918
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MfeI CAATTG 2 cut(s) 405, 1026
MhlI GDGCHC 3 cut(s) 11, 246, 519
MlsI TGGCCA 2 cut(s) 125, 447
MluCI AATT 7 cut(s) 343, 405, 568, 675, 738, 856, 1026
MluNI TGGCCA 2 cut(s) 125, 447
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 995
Mox20I TGGCCA 2 cut(s) 125, 447
Mph1103I ATGCAT 1 cut(s) 667
MscI TGGCCA 2 cut(s) 125, 447
MseI TTAA 3 cut(s) 476, 837, 863
MslI CAYNNNNRTG 2 cut(s) 959, 995
Msp20I TGGCCA 2 cut(s) 125, 447
MspI CCGG 4 cut(s) 214, 362, 510, 769
MspR9I CCNGG 5 cut(s) 362, 554, 603, 770, 833
MunI CAATTG 2 cut(s) 405, 1026
MvaI CCWGG 3 cut(s) 554, 603, 833
MwoI GCNNNNNNNGC 3 cut(s) 75, 410, 434
NciI CCSGG 2 cut(s) 362, 770
NcoI CCATGG 1 cut(s) 399
NdeI CATATG 1 cut(s) 505
NdeII GATC 1 cut(s) 294
NlaIII CATG 9 cut(s) 19, 85, 403, 551, 812, 820, 851, 964, 1080
NlaIV GGNNCC 2 cut(s) 576, 901
NmuCI GTSAC 2 cut(s) 177, 918
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 2 cut(s) 820, 1080
OliI CACNNNNGTG 1 cut(s) 995
PciI ACATGT 2 cut(s) 816, 1076
PfeI GAWTC 3 cut(s) 313, 395, 680
PfoI TCCNGGA 1 cut(s) 601
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PscI ACATGT 2 cut(s) 816, 1076
Psp124BI GAGCTC 3 cut(s) 11, 246, 519
Psp6I CCWGG 3 cut(s) 552, 601, 831
PspGI CCWGG 3 cut(s) 552, 601, 831
PspN4I GGNNCC 2 cut(s) 576, 901
PspPI GGNCC 2 cut(s) 50, 968
PstI CTGCAG 2 cut(s) 259, 700
RsaI GTAC 2 cut(s) 576, 846
RsaNI GTAC 2 cut(s) 575, 845
RseI CAYNNNNRTG 2 cut(s) 959, 995
SacI GAGCTC 3 cut(s) 11, 246, 519
SaqAI TTAA 3 cut(s) 476, 837, 863
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 2 cut(s) 50, 968
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 5 cut(s) 362, 554, 603, 770, 833
SduI GDGCHC 3 cut(s) 11, 246, 519
SfaNI GCATC 6 cut(s) 52, 415, 652, 767, 835, 1086
SfcI CTRYAG 2 cut(s) 255, 696
SinI GGWCC 2 cut(s) 50, 968
SmiMI CAYNNNNRTG 2 cut(s) 959, 995
Sse9I AATT 7 cut(s) 343, 405, 568, 675, 738, 856, 1026
SsiI CCGC 1 cut(s) 616
SspMI CTAG 4 cut(s) 12, 390, 522, 926
SstI GAGCTC 3 cut(s) 11, 246, 519
StyD4I CCNGG 5 cut(s) 360, 552, 601, 768, 831
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 337, 545
TaiI ACGT 1 cut(s) 566
TaqI TCGA 1 cut(s) 1008
TasI AATT 7 cut(s) 343, 405, 568, 675, 738, 856, 1026
TfiI GAWTC 3 cut(s) 313, 395, 680
Tru1I TTAA 3 cut(s) 476, 837, 863
Tru9I TTAA 3 cut(s) 476, 837, 863
TscAI CASTG 1 cut(s) 1002
TseFI GTSAC 2 cut(s) 177, 918
Tsp45I GTSAC 2 cut(s) 177, 918
TspDTI ATGAA 3 cut(s) 481, 849, 867
TspRI CASTG 1 cut(s) 1002
VpaK11BI GGWCC 2 cut(s) 50, 968
XapI RAATTY 2 cut(s) 568, 856
XceI RCATGY 2 cut(s) 820, 1080
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 4 cut(s) 12, 390, 522, 926
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.