Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0017662

Physical Location & Seq

Genomic Coordinates Reverse (-)

20784655 .. 20786608

Gene Structure 1954 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1130200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 645 bp

ATGGGCTGTGTTTGCATGAAGCAGAGACTTGAATGTGAAAACTTAGCAGCAGTTCTTGCTTCTCAAACCTGCTTTAAGGTAAATGAGGTTAAATCTTTGTACGAGTTGTTCAGGAAGTTGAGCAGTTCTATAGTTGATGATGGCCTTATAAGCAAAGAAGAGTTTCAGCTCGGATTGTTCAGGAACAGCAAAACACAAAGTCTGTTTGCAGATAGGGTCTTCCATATGTTTGACACGAAACGTGATGGGGTGATAGGATTCGAAGAGTTTGTTCGATGTCTGAGCGTGTTCCACCCGGAAGCACCCCAAGCAGAGAAGGCTGCATTTGCATATCAGCTATATGATATATGGCAGACAGGCTTCATCGAACGCGAAGAGGTGAAAGAAATGGTTCTCGCGCTTTTGAAAGAGTCGGACTTGATCCTGTCTGATGACATTGTTGAAGCGATAATTGATAAGACATTTGAGGAAGCAGACTTAAAGGGAGACGGAAAGATTGATCTTGAAGAGTGGAATGAGCTCGTGAATGGCAATCCGTCCTTATTGAAGAACATGACAATTCCATACCTCAAGGACATCACGACATCATTTCCTAGTTTCGTGATGAAAACAGACAATGAAGACGACAATAACATCTACCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005488 GO:0005509 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0019722 GO:0019932 GO:0023052 GO:0035556 GO:0042221 GO:0043167 GO:0043169 GO:0046872 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04010 ko04020 ko04022 ko04114 ko04218 ko04310 ko04360 ko04370 ko04380 ko04650 ko04658 ko04659 ko04660 ko04662 ko04720 ko04724 ko04921 ko04922 ko04924 ko05010 ko05014 ko05031 ko05152 ko05166 ko05167 map04010 map04020 map04022 map04114 map04218 map04310 map04360 map04370 map04380 map04650 map04658 map04659 map04660 map04662 map04720 map04724 map04921 map04922 map04924 map05010 map05014 map05031 map05152 map05166 map05167

Pfam Domains

Protein Families

EF-hand_1 EF-hand_4 EF-hand_5 EF-hand_7

Protein Analysis

215

Amino Acids

24.53

Weight (kDa)

4.54

Isoelectric Point (pI)

32.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_1	PF00036	71 - 95	8.5e-06	EF hand domain
EF-hand_7	PF13499	105 - 170	3.9e-08	EF-hand domain pair
EF-hand_5	PF13202	151 - 175	1.6e-07	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0017662)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g04810
malus_domestica	MD14G1130200.v1.1
prunus_persica	Prupe.5G122100_v2.0.a1 Prupe.5G122100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0194321
rosa_multiflora	Rmu_sc0000901.1_g000015
rosa_roxburghii	Rroxscaffold_3G00261420
rosa_rugosa	Rorug07G0015400
rosa_samantha	Rh7AG142400 Rh7CG146500 Rh7DG144100
rosa_wichuraiana	Rw7G012190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	149
Acc36I	ACCTGC	1 cut(s)	77
AccII	CGCG	2 cut(s)	372, 398
AclWI	GGATC	1 cut(s)	415
AfaI	GTAC	1 cut(s)	101
AgsI	TTSAA	5 cut(s)	32, 406, 443, 506, 547
AluBI	AGCT	3 cut(s)	169, 337, 520
AluI	AGCT	3 cut(s)	169, 337, 520
Alw21I	GWGCWC	1 cut(s)	522
Alw26I	GTCTC	2 cut(s)	19, 480
AlwI	GGATC	1 cut(s)	415
AoxI	GGCC	1 cut(s)	142
ApeKI	GCWGC	2 cut(s)	47, 320
Asp700I	GAANNNNTTC	2 cut(s)	162, 390
AspLEI	GCGC	1 cut(s)	400
AsuC2I	CCSGG	1 cut(s)	296
AsuHPI	GGTGA	2 cut(s)	262, 391
AsuII	TTCGAA	1 cut(s)	261
BanII	GRGCYC	1 cut(s)	522
BauI	CACGAG	1 cut(s)	521
BbsI	GAAGAC	2 cut(s)	211, 627
Bbv12I	GWGCWC	1 cut(s)	522
BbvI	GCAGC	2 cut(s)	59, 307
BccI	CCATC	2 cut(s)	134, 239
BcnI	CCSGG	1 cut(s)	296
BcoDI	GTCTC	2 cut(s)	19, 480
BfaI	CTAG	1 cut(s)	594
BfmI	CTRYAG	1 cut(s)	129
BfuAI	ACCTGC	1 cut(s)	77
BisI	GCNGC	2 cut(s)	48, 321
BlsI	GCNGC	2 cut(s)	49, 322
Bme1390I	CCNGG	1 cut(s)	296
BmrFI	CCNGG	1 cut(s)	296
BpiI	GAAGAC	2 cut(s)	211, 627
Bpu14I	TTCGAA	1 cut(s)	261
BpuEI	CTTGAG	1 cut(s)	554
BpuMI	CCSGG	1 cut(s)	296
BseMII	CTCAG	1 cut(s)	272
BseXI	GCAGC	2 cut(s)	59, 307
Bsh1236I	CGCG	2 cut(s)	372, 398
BshFI	GGCC	1 cut(s)	144
BsiHKAI	GWGCWC	1 cut(s)	522
BsiSI	CCGG	1 cut(s)	296
BsmAI	GTCTC	2 cut(s)	19, 480
BsmBI	CGTCTC	1 cut(s)	480
BsnI	GGCC	1 cut(s)	144
Bsp119I	TTCGAA	1 cut(s)	261
Bsp1286I	GDGCHC	1 cut(s)	522
Bsp143I	GATC	2 cut(s)	420, 499
BspANI	GGCC	1 cut(s)	144
BspCNI	CTCAG	1 cut(s)	273
BspFNI	CGCG	2 cut(s)	372, 398
BspMI	ACCTGC	1 cut(s)	77
BspPI	GGATC	1 cut(s)	415
BspT104I	TTCGAA	1 cut(s)	261
BssMI	GATC	2 cut(s)	420, 499
BssSI	CACGAG	1 cut(s)	521
Bst2BI	CACGAG	1 cut(s)	521
Bst6I	CTCTTC	4 cut(s)	153, 258, 369, 501
BstAPI	GCANNNNNTGC	1 cut(s)	56
BstBI	TTCGAA	1 cut(s)	261
BstDEI	CTNAG	2 cut(s)	43, 281
BstFNI	CGCG	2 cut(s)	372, 398
BstHHI	GCGC	1 cut(s)	400
BstKTI	GATC	2 cut(s)	423, 502
BstMAI	GTCTC	2 cut(s)	19, 480
BstMBI	GATC	2 cut(s)	420, 499
BstMWI	GCNNNNNNNGC	6 cut(s)	12, 56, 150, 308, 317, 326
BstSCI	CCNGG	1 cut(s)	294
BstSFI	CTRYAG	1 cut(s)	129
BstUI	CGCG	2 cut(s)	372, 398
BstV1I	GCAGC	2 cut(s)	59, 307
BstV2I	GAAGAC	2 cut(s)	211, 627
BsuRI	GGCC	1 cut(s)	144
BveI	ACCTGC	1 cut(s)	77
CfoI	GCGC	1 cut(s)	400
Csp6I	GTAC	1 cut(s)	100
CviAII	CATG	2 cut(s)	16, 553
CviJI	RGCY	7 cut(s)	6, 144, 169, 320, 337, 360, 520
CviKI_1	RGCY	7 cut(s)	6, 144, 169, 320, 337, 360, 520
CviQI	GTAC	1 cut(s)	100
DdeI	CTNAG	2 cut(s)	43, 281
DpnI	GATC	2 cut(s)	422, 501
DpnII	GATC	2 cut(s)	420, 499
Eam1104I	CTCTTC	4 cut(s)	153, 258, 369, 501
EarI	CTCTTC	4 cut(s)	153, 258, 369, 501
Ecl136II	GAGCTC	1 cut(s)	520
Eco24I	GRGCYC	1 cut(s)	522
Eco53kI	GAGCTC	1 cut(s)	520
EcoICRI	GAGCTC	1 cut(s)	520
EcoT38I	GRGCYC	1 cut(s)	522
Esp3I	CGTCTC	1 cut(s)	480
FaeI	CATG	2 cut(s)	19, 556
FatI	CATG	2 cut(s)	15, 552
FauNDI	CATATG	1 cut(s)	225
Fnu4HI	GCNGC	2 cut(s)	48, 321
FriOI	GRGCYC	1 cut(s)	522
Fsp4HI	GCNGC	2 cut(s)	48, 321
FspBI	CTAG	1 cut(s)	594
GlaI	GCGC	1 cut(s)	399
GluI	GCNGC	2 cut(s)	48, 321
HaeIII	GGCC	1 cut(s)	144
HapII	CCGG	1 cut(s)	296
HhaI	GCGC	1 cut(s)	400
Hin1II	CATG	2 cut(s)	19, 556
Hin6I	GCGC	1 cut(s)	398
HinP1I	GCGC	1 cut(s)	398
HinfI	GANTC	2 cut(s)	258, 410
HpaII	CCGG	1 cut(s)	296
HphI	GGTGA	2 cut(s)	262, 391
Hpy188I	TCNGA	4 cut(s)	173, 282, 415, 430
Hpy188III	TCNNGA	6 cut(s)	112, 181, 503, 523, 580, 601
HpyAV	CCTTC	1 cut(s)	310
HpyCH4IV	ACGT	1 cut(s)	241
HpyCH4V	TGCA	4 cut(s)	15, 209, 323, 329
HpyF10VI	GCNNNNNNNGC	6 cut(s)	12, 56, 150, 308, 317, 326
HpyF3I	CTNAG	2 cut(s)	43, 281
HpySE526I	ACGT	1 cut(s)	241
Hsp92II	CATG	2 cut(s)	19, 556
HspAI	GCGC	1 cut(s)	398
Kzo9I	GATC	2 cut(s)	420, 499
LpnPI	CCDG	6 cut(s)	82, 97, 166, 309, 342, 437
Lsp1109I	GCAGC	2 cut(s)	59, 307
MaeI	CTAG	1 cut(s)	594
MaeII	ACGT	1 cut(s)	241
MalI	GATC	2 cut(s)	422, 501
MboI	GATC	2 cut(s)	420, 499
MboII	GAAGA	7 cut(s)	170, 211, 275, 386, 518, 559, 632
MhlI	GDGCHC	1 cut(s)	522
MluCI	AATT	2 cut(s)	450, 558
MlyI	GAGTC	1 cut(s)	419
MmeI	TCCRAC	1 cut(s)	393
MnlI	CCTC	4 cut(s)	79, 370, 460, 578
MroXI	GAANNNNTTC	2 cut(s)	162, 390
MseI	TTAA	3 cut(s)	75, 90, 479
MspI	CCGG	1 cut(s)	296
MspR9I	CCNGG	1 cut(s)	296
MvnI	CGCG	2 cut(s)	372, 398
MwoI	GCNNNNNNNGC	6 cut(s)	12, 56, 150, 308, 317, 326
NciI	CCSGG	1 cut(s)	296
NdeI	CATATG	1 cut(s)	225
NdeII	GATC	2 cut(s)	420, 499
NlaIII	CATG	2 cut(s)	19, 556
NspV	TTCGAA	1 cut(s)	261
PdmI	GAANNNNTTC	2 cut(s)	162, 390
PfeI	GAWTC	1 cut(s)	258
PkrI	GCNGC	2 cut(s)	49, 322
PleI	GAGTC	1 cut(s)	418
PpsI	GAGTC	1 cut(s)	418
PsiI	TTATAA	1 cut(s)	149
Psp124BI	GAGCTC	1 cut(s)	522
PsrI	GAACNNNNNNTAC	2 cut(s)	92, 124
RsaI	GTAC	1 cut(s)	101
RsaNI	GTAC	1 cut(s)	100
SacI	GAGCTC	1 cut(s)	522
SaqAI	TTAA	3 cut(s)	75, 90, 479
SatI	GCNGC	2 cut(s)	48, 321
Sau3AI	GATC	2 cut(s)	420, 499
SchI	GAGTC	1 cut(s)	419
ScrFI	CCNGG	1 cut(s)	296
SduI	GDGCHC	1 cut(s)	522
SfcI	CTRYAG	1 cut(s)	129
SfuI	TTCGAA	1 cut(s)	261
SmlI	CTYRAG	1 cut(s)	569
SmoI	CTYRAG	1 cut(s)	569
Sse9I	AATT	2 cut(s)	450, 558
SspMI	CTAG	1 cut(s)	594
SstI	GAGCTC	1 cut(s)	522
StyD4I	CCNGG	1 cut(s)	294
TaiI	ACGT	1 cut(s)	244
TaqI	TCGA	3 cut(s)	261, 274, 366
TasI	AATT	2 cut(s)	450, 558
TfiI	GAWTC	1 cut(s)	258
Tru1I	TTAA	3 cut(s)	75, 90, 479
Tru9I	TTAA	3 cut(s)	75, 90, 479
TseI	GCWGC	2 cut(s)	47, 320
TspDTI	ATGAA	4 cut(s)	32, 352, 620, 633
TspGWI	ACGGA	2 cut(s)	504, 525
XmnI	GAANNNNTTC	2 cut(s)	162, 390
XspI	CTAG	1 cut(s)	594

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.