Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0011226

Physical Location & Seq

Genomic Coordinates Reverse (-)

21241019 .. 21256565

Gene Structure 15547 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1134200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGAGGCTGCTGAAGGTCGCAACCTGTAATCTGAACCAATGGGCAATGGACTTCGACTGCAATTTGAAGAACATCAAGGAGTCCATAGTTCGGGCTAAGGATGCCGGCGCCGTCATTCGACTCGGTCCCGAGCTTGAGATCACTGGATATGGCTGCGAAGACCATTTCCTCGAGCTCGACACTGTTAATCATGCATGGGAATGCTTGAAAGAGCTGTTAGTTGGGGATTGGACTGATGGCATATTGTGCAGCTTTGGTATGCCTGTGATCAAAGGGTCGGAGCGTTACAATTGTCAGATTATTTGTATGAACAGAAAGATTATCATGATACGCCCGAAGATGTGGCTTGCGAATGATGGAAATTACAGGGAACTTCGTTGGTTTACGGCATGGAAACAAAGAGACCAACTTGTGGACTTTCAGTTGCCAAATGAAATTGCTGAAACTTTGTCGCAGGAATCTGCGCCTTTCGGTTATGGGTACATACAGTTTCTAGACACAGCTGTTGCTGCTGAAGTTTGTGAAGAACTTTTTACTCCTATTCCTCCTCATGCCGAGCTTGCATTGAATGGGGTTGAAGTTTTTATGAATGCAAGTGGAAGTCATCACCAACTTAGAAAGCTTGATATTCGTCTTCGTGCTTTTATAGGTGCTACTCACACTCGTGGAGGGGTATACATGTACAGCAATCAGCAGGGTTGTGATGGTGGTCGCCTTTACTATGATGGATGTGCCAGTGTTGTCGTAAATGGAGATCTAGTTGCTCAAGGCTCACAATTTTCTTTGAAGGATGTTGAGGTTGTGATTGCCCAAATTGATTTAGATGCGGTTGCTAGTCTTAGAGGATCTATAAGTAGCTTCCAAGAGCAGGCGAGCTGCAAAACCAGGGTTCCTTTTGTGGAGGCACGATACAGCCTTTGTCAGTCTTTTAATCTGAAAATGTGCCTTTCTAGTCCTCTGAAGATTAAGTATCACTCTCCTGAGGAGGAAATAGCCTTTGGTCCTGGTTGCTGGTTATGGGACTATTTAAGAAGAAGTGGAGCTTCTGGGTTTTTGCTTCCTCTTTCTGGTGGAGCAGATAGCTCCTCAGTAGCTGCTATAGTTGGTTGCATGTGCCAGCTTGTTGTAAAAGAAATTGCAAACGGGGATGAACAAGTCAAGGTTGACGCGATACGCATTGGACAATACAAGGATGGACAGTATCCTACTGACAGCAAAGAATTCGCAAAACGCATATTTTATACTGTTTTCATGGGATCTGAAAACAGCTCTGAAGCAACAAAGTCATGGGCGAAAGTTCTAGCTGATGAAATCGGCTCGTGGCATCTTGACGTTCCTATAGATGGTGTTGTTTCAGCGCTTCTGTCATTATTTCAAACAGTAACAGGAAAACGACCAAAATACAAGGTAGATGGTGGATCTAACGTTGAGAACTTGGGTTTGCAAAACATTCAAGCTAGAATAAGGATGGTGCTGGCTTTTATGTTTGCATCACTCCTACCTTGGGTTCATAATAAACCTGGGTTTTATCTTGTTTTGGGTAGCTCCAATGTGGATGAAGCATTGCGTGGTTATCTCACCAAGTATGACTGCAGCGCAGCAGATATAAATCCTATCGGAAGCATTAGCAAGCAGGACCTTCGAGCATTTTTGCGATGGGCTGCCACGCATCTTAGTTATGTGTCCTTGGCTGATATTGAAGCAGCTCCACCCACTGCTGAACTGGAACCTATACGTTCCAATTACAGTCAGCTGGATGAAGTAGACATGGGAATGACATATCAGGAACTCTCGGTCTATGGAAGGATGCGCAAAATTTTCCGCTGTGGTCCCGTATCCATGTTCAAGAATCTCTGCTACAGATGGGGTGCAAAATTGACTCCACGAGAGGTGGCCGAGAAAGTGAAGCACTTTTTCAAGTACTACTCCATCAATCGACACAAAATGACCGTGCTAACACCTTCGTATCATGCTGAGAGTTATTCACCCGAGGATAACCGGTTTGACCTTCGCCAATTTCTATACAACGCGAGATGGCCATTTCAATTCCGGAAGATTGATGATCTCGTACGTGAGTTGGACGGTGACAGGGTTCATTTGGGAGATTCAAGTGACCAAGACAAGTTGGTTGATATTTCACATGGTGCAGGTGGGATGGGAGTTGTGGCAGCAGGCTCAGGCAATCCAAATGTTGGACTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003952 GO:0004359 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009165 GO:0009435 GO:0009987 GO:0016787 GO:0016810 GO:0016811 GO:0016874 GO:0016879 GO:0016884 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019637 GO:0019674 GO:0034641 GO:0034654 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046496 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072524 GO:0072525 GO:0090407 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00760 ko01100 map00760 map01100

Pfam Domains

Protein Families

CN_hydrolase NAD_synthase

Protein Analysis

734

Amino Acids

81.99

Weight (kDa)

5.99

Isoelectric Point (pI)

37.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CN_hydrolase	PF00795	5 - 281	1e-30	Carbon-nitrogen hydrolase
NAD_synthase	PF02540	338 - 598	1.2e-26	NAD synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011226)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55090
fragaria_vesca	FvH4_5g11490 FvH4_5g11490 FvH4_5g11490
malus_domestica	MD06G1113100.v1.1 MD14G1134200.v1.1
prunus_persica	Prupe.5G126000_v2.0.a1
pyrus_communis	pycom06g10580
rosa_chinensis	RchiOBHm_Chr7g0186311
rosa_laevigata	RLG00000004850
rosa_multiflora	Rmu_sc0000645.1_g000051
rosa_roxburghii	Rroxscaffold_3G00268230
rosa_rugosa	Rorug06G0473200 Rorug06G0473300 Rorug06G0473400 Rorug06G0473400
rosa_samantha	Rh7AG067800 Rh7BG083600 Rh7DG080800
rosa_wichuraiana	Rw7G006720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2138
Acc16I	TGCGCA	1 cut(s)	1811
Acc36I	ACCTGC	1 cut(s)	2138
AccB1I	GGYRCC	1 cut(s)	107
AccB7I	CCANNNNNTGG	2 cut(s)	412, 2192
AccI	GTMKAC	2 cut(s)	675, 1764
AccII	CGCG	2 cut(s)	1169, 2030
AccIII	TCCGGA	1 cut(s)	2049
AciI	CCGC	2 cut(s)	827, 1822
AclI	AACGTT	1 cut(s)	1425
AclWI	GGATC	3 cut(s)	853, 1264, 1426
AcoI	YGGCCR	2 cut(s)	1893, 2036
AcsI	RAATTY	2 cut(s)	1220, 1815
AcuI	CTGAAG	4 cut(s)	32, 534, 980, 1293
AcyI	GRCGYC	1 cut(s)	108
AdeI	CACNNNGTG	1 cut(s)	2144
AfaI	GTAC	4 cut(s)	482, 683, 1922, 2070
AfeI	AGCGCT	1 cut(s)	1359
AfiI	CCNNNNNNNGG	7 cut(s)	90, 412, 868, 1067, 1343, 1504, 2192
AflIII	ACRYGT	1 cut(s)	678
AgeI	ACCGGT	1 cut(s)	1998
AjnI	CCWGG	3 cut(s)	884, 1003, 1519
AjuI	GAANNNNNNNTTGG	2 cut(s)	981, 1013
AleI	CACNNNNGTG	1 cut(s)	663
Alw21I	GWGCWC	1 cut(s)	177
Alw26I	GTCTC	1 cut(s)	396
AlwI	GGATC	3 cut(s)	853, 1264, 1426
AlwNI	CAGNNNCTG	1 cut(s)	1094
Ama87I	CYCGRG	3 cut(s)	128, 170, 1988
Aor13HI	TCCGGA	1 cut(s)	2049
Aor51HI	AGCGCT	1 cut(s)	1359
AoxI	GGCC	2 cut(s)	1893, 2036
ApoI	RAATTY	2 cut(s)	1220, 1815
AsiGI	ACCGGT	1 cut(s)	1998
AspLEI	GCGC	5 cut(s)	110, 466, 1360, 1598, 1812
AspS9I	GGNCC	4 cut(s)	125, 1001, 1636, 1829
AsuHPI	GGTGA	4 cut(s)	599, 1570, 1977, 2096
AvaI	CYCGRG	3 cut(s)	128, 170, 1988
AvaII	GGWCC	4 cut(s)	125, 1001, 1636, 1829
AxyI	CCTNAGG	1 cut(s)	981
BaeI	ACNNNNGTAYC	2 cut(s)	1949, 1982
BalI	TGGCCA	1 cut(s)	2038
BanI	GGYRCC	1 cut(s)	107
BanII	GRGCYC	1 cut(s)	177
BauI	CACGAG	3 cut(s)	663, 1318, 1884
BbsI	GAAGAC	2 cut(s)	165, 626
Bbv12I	GWGCWC	1 cut(s)	177
BceAI	ACGGC	2 cut(s)	95, 402
BcgI	CGANNNNNNTGC	4 cut(s)	1204, 1238, 1622, 1656
BciT130I	CCWGG	3 cut(s)	886, 1005, 1521
BciVI	GTATCC	2 cut(s)	1212, 1846
BclI	TGATCA	1 cut(s)	267
BcoDI	GTCTC	1 cut(s)	396
BfaI	CTAG	7 cut(s)	494, 758, 834, 951, 1301, 1458, 2200
BfmI	CTRYAG	4 cut(s)	1098, 1338, 1591, 1858
BfoI	RGCGCY	2 cut(s)	111, 1361
BfuAI	ACCTGC	1 cut(s)	2138
BfuI	GTATCC	2 cut(s)	1212, 1846
BglII	AGATCT	1 cut(s)	754
BmcAI	AGTACT	1 cut(s)	1922
Bme1390I	CCNGG	3 cut(s)	886, 1005, 1521
Bme18I	GGWCC	4 cut(s)	125, 1001, 1636, 1829
BmeT110I	CYCGRG	3 cut(s)	128, 170, 1988
BmgT120I	GGNCC	4 cut(s)	125, 1001, 1636, 1829
BmiI	GGNNCC	5 cut(s)	109, 127, 891, 1728, 1831
BmrFI	CCNGG	3 cut(s)	886, 1005, 1521
BmsI	GCATC	6 cut(s)	91, 814, 1333, 1499, 1678, 1797
BpiI	GAAGAC	2 cut(s)	165, 626
Bpu10I	CCTNAGC	2 cut(s)	96, 2176
BpuEI	CTTGAG	2 cut(s)	155, 750
BsaAI	YACGTR	1 cut(s)	2072
BsaBI	GATNNNNATC	1 cut(s)	1608
BsaHI	GRCGYC	1 cut(s)	108
BsaI	GGTCTC	1 cut(s)	396
BsaJI	CCNNGG	5 cut(s)	885, 1502, 1520, 1686, 1989
BsaWI	WCCGGW	2 cut(s)	1998, 2049
BsaXI	ACNNNNNCTCC	2 cut(s)	744, 774
Bsc4I	CCNNNNNNNGG	7 cut(s)	90, 412, 868, 1067, 1343, 1504, 2192
Bse118I	RCCGGY	2 cut(s)	104, 1998
Bse1I	ACTGG	3 cut(s)	148, 735, 1728
Bse21I	CCTNAGG	1 cut(s)	981
Bse3DI	GCAATG	2 cut(s)	51, 1562
Bse8I	GATNNNNATC	1 cut(s)	1608
BseAI	TCCGGA	1 cut(s)	2049
BseBI	CCWGG	3 cut(s)	886, 1005, 1521
BseDI	CCNNGG	5 cut(s)	885, 1502, 1520, 1686, 1989
BseJI	GATNNNNATC	1 cut(s)	1608
BseLI	CCNNNNNNNGG	7 cut(s)	90, 412, 868, 1067, 1343, 1504, 2192
BseMI	GCAATG	2 cut(s)	51, 1562
BseMII	CTCAG	4 cut(s)	972, 1101, 1965, 2190
BseNI	ACTGG	3 cut(s)	148, 735, 1728
BseRI	GAGGAG	3 cut(s)	537, 998, 1075
BsgI	GTGCAG	2 cut(s)	268, 2166
Bsh1236I	CGCG	2 cut(s)	1169, 2030
BshFI	GGCC	2 cut(s)	1895, 2038
BshNI	GGYRCC	1 cut(s)	107
BshTI	ACCGGT	1 cut(s)	1998
BsiHKAI	GWGCWC	1 cut(s)	177
BsiHKCI	CYCGRG	3 cut(s)	128, 170, 1988
BsiSI	CCGG	3 cut(s)	105, 1999, 2050
BsiWI	CGTACG	1 cut(s)	2068
BslFI	GGGAC	3 cut(s)	111, 1034, 1815
BslI	CCNNNNNNNGG	7 cut(s)	90, 412, 868, 1067, 1343, 1504, 2192
BsmAI	GTCTC	1 cut(s)	396
BsmFI	GGGAC	3 cut(s)	111, 1034, 1815
BsmI	GAATGC	2 cut(s)	206, 595
BsnI	GGCC	2 cut(s)	1895, 2038
Bso31I	GGTCTC	1 cut(s)	396
BsoBI	CYCGRG	3 cut(s)	128, 170, 1988
Bsp1286I	GDGCHC	1 cut(s)	177
Bsp13I	TCCGGA	1 cut(s)	2049
Bsp1407I	TGTACA	1 cut(s)	681
Bsp143I	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
BspACI	CCGC	2 cut(s)	827, 1822
BspANI	GGCC	2 cut(s)	1895, 2038
BspCNI	CTCAG	4 cut(s)	973, 1100, 1966, 2189
BspEI	TCCGGA	1 cut(s)	2049
BspFNI	CGCG	2 cut(s)	1169, 2030
BspHI	TCATGA	1 cut(s)	324
BspLI	GGNNCC	5 cut(s)	109, 127, 891, 1728, 1831
BspMAI	CTGCAG	1 cut(s)	1595
BspMI	ACCTGC	1 cut(s)	2138
BspPI	GGATC	3 cut(s)	853, 1264, 1426
BspT107I	GGYRCC	1 cut(s)	107
BspTNI	GGTCTC	1 cut(s)	396
BsrDI	GCAATG	2 cut(s)	51, 1562
BsrFI	RCCGGY	2 cut(s)	104, 1998
BsrGI	TGTACA	1 cut(s)	681
BsrI	ACTGG	3 cut(s)	148, 735, 1728
BssAI	RCCGGY	2 cut(s)	104, 1998
BssECI	CCNNGG	5 cut(s)	885, 1502, 1520, 1686, 1989
BssMI	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
BssNAI	GTATAC	1 cut(s)	676
BssNI	GRCGYC	1 cut(s)	108
BssSI	CACGAG	3 cut(s)	663, 1318, 1884
BssT1I	CCWWGG	2 cut(s)	1502, 1686
Bst1107I	GTATAC	1 cut(s)	676
Bst2BI	CACGAG	3 cut(s)	663, 1318, 1884
Bst2UI	CCWGG	3 cut(s)	886, 1005, 1521
Bst4CI	ACNGT	8 cut(s)	184, 489, 1200, 1246, 1381, 1748, 1951, 2084
BstACI	GRCGYC	1 cut(s)	108
BstAPI	GCANNNNNTGC	1 cut(s)	246
BstAUI	TGTACA	1 cut(s)	681
BstBAI	YACGTR	1 cut(s)	2072
BstC8I	GCNNGC	9 cut(s)	106, 348, 561, 870, 874, 1118, 1476, 1631, 2173
BstDEI	CTNAG	8 cut(s)	96, 614, 839, 981, 1087, 1673, 1974, 2176
BstFNI	CGCG	2 cut(s)	1169, 2030
BstH2I	RGCGCY	2 cut(s)	111, 1361
BstHHI	GCGC	5 cut(s)	110, 466, 1360, 1598, 1812
BstKTI	GATC	7 cut(s)	141, 270, 757, 848, 1259, 1421, 2065
BstMAI	GTCTC	1 cut(s)	396
BstMBI	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
BstMWI	GCNNNNNNNGC	4 cut(s)	101, 246, 509, 560
BstNI	CCWGG	3 cut(s)	886, 1005, 1521
BstNSI	RCATGY	2 cut(s)	682, 1114
BstSCI	CCNGG	3 cut(s)	884, 1003, 1519
BstSFI	CTRYAG	4 cut(s)	1098, 1338, 1591, 1858
BstUI	CGCG	2 cut(s)	1169, 2030
BstV2I	GAAGAC	2 cut(s)	165, 626
BstX2I	RGATCY	4 cut(s)	754, 845, 1256, 1418
BstYI	RGATCY	4 cut(s)	754, 845, 1256, 1418
BstZ17I	GTATAC	1 cut(s)	676
Bsu36I	CCTNAGG	1 cut(s)	981
BsuI	GTATCC	2 cut(s)	1212, 1846
BsuRI	GGCC	2 cut(s)	1895, 2038
BtgZI	GCGATG	1 cut(s)	1669
BtsI	GCAGTG	1 cut(s)	1713
BtsIMutI	CAGTG	4 cut(s)	141, 180, 742, 1713
BveI	ACCTGC	1 cut(s)	2138
Cac8I	GCNNGC	9 cut(s)	106, 348, 561, 870, 874, 1118, 1476, 1631, 2173
CaiI	CAGNNNCTG	1 cut(s)	1094
CciI	TCATGA	1 cut(s)	324
CfoI	GCGC	5 cut(s)	110, 466, 1360, 1598, 1812
Cfr10I	RCCGGY	2 cut(s)	104, 1998
Cfr13I	GGNCC	4 cut(s)	125, 1001, 1636, 1829
CseI	GACGC	1 cut(s)	1175
Csp6I	GTAC	4 cut(s)	481, 682, 1921, 2069
CspAI	ACCGGT	1 cut(s)	1998
CviQI	GTAC	4 cut(s)	481, 682, 1921, 2069
DdeI	CTNAG	8 cut(s)	96, 614, 839, 981, 1087, 1673, 1974, 2176
DinI	GGCGCC	1 cut(s)	109
DpnI	GATC	7 cut(s)	140, 269, 756, 847, 1258, 1420, 2064
DpnII	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
DraIII	CACNNNGTG	1 cut(s)	2144
EaeI	YGGCCR	2 cut(s)	1893, 2036
Ecl136II	GAGCTC	1 cut(s)	175
Eco130I	CCWWGG	2 cut(s)	1502, 1686
Eco24I	GRGCYC	1 cut(s)	177
Eco31I	GGTCTC	1 cut(s)	396
Eco47I	GGWCC	4 cut(s)	125, 1001, 1636, 1829
Eco47III	AGCGCT	1 cut(s)	1359
Eco53kI	GAGCTC	1 cut(s)	175
Eco57I	CTGAAG	4 cut(s)	32, 534, 980, 1293
Eco81I	CCTNAGG	1 cut(s)	981
Eco88I	CYCGRG	3 cut(s)	128, 170, 1988
EcoICRI	GAGCTC	1 cut(s)	175
EcoO109I	RGGNCCY	1 cut(s)	1636
EcoRI	GAATTC	1 cut(s)	1220
EcoRII	CCWGG	3 cut(s)	884, 1003, 1519
EcoT14I	CCWWGG	2 cut(s)	1502, 1686
EcoT22I	ATGCAT	1 cut(s)	196
EcoT38I	GRGCYC	1 cut(s)	177
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	2 cut(s)	1502, 1686
FalI	AAGNNNNNCTT	2 cut(s)	1027, 1059
FaqI	GGGAC	3 cut(s)	111, 1034, 1815
FbaI	TGATCA	1 cut(s)	267
FblI	GTMKAC	2 cut(s)	675, 1764
FriOI	GRGCYC	1 cut(s)	177
FspBI	CTAG	7 cut(s)	494, 758, 834, 951, 1301, 1458, 2200
FspI	TGCGCA	1 cut(s)	1811
GlaI	GCGC	5 cut(s)	109, 465, 1359, 1597, 1811
HaeII	RGCGCY	2 cut(s)	111, 1361
HaeIII	GGCC	2 cut(s)	1895, 2038
HapII	CCGG	3 cut(s)	105, 1999, 2050
HgaI	GACGC	1 cut(s)	1175
HhaI	GCGC	5 cut(s)	110, 466, 1360, 1598, 1812
Hin1I	GRCGYC	1 cut(s)	108
Hin6I	GCGC	5 cut(s)	108, 464, 1358, 1596, 1810
HinP1I	GCGC	5 cut(s)	108, 464, 1358, 1596, 1810
HincII	GTYRAC	1 cut(s)	1165
HindII	GTYRAC	1 cut(s)	1165
HindIII	AAGCTT	1 cut(s)	620
HinfI	GANTC	7 cut(s)	80, 120, 458, 1849, 1879, 2105, 2196
HpaII	CCGG	3 cut(s)	105, 1999, 2050
HphI	GGTGA	4 cut(s)	599, 1570, 1977, 2096
Hpy166II	GTNNAC	5 cut(s)	384, 415, 676, 1165, 1765
Hpy188I	TCNGA	8 cut(s)	33, 280, 297, 936, 960, 1261, 1273, 1619
Hpy188III	TCNNGA	8 cut(s)	128, 325, 494, 980, 1328, 1784, 1846, 2050
Hpy8I	GTNNAC	5 cut(s)	384, 415, 676, 1165, 1765
HpyAV	CCTTC	6 cut(s)	7, 781, 1649, 1797, 1971, 2018
HpyCH4III	ACNGT	8 cut(s)	184, 489, 1200, 1246, 1381, 1748, 1951, 2084
HpyCH4IV	ACGT	4 cut(s)	1332, 1425, 1735, 2071
HpyF10VI	GCNNNNNNNGC	4 cut(s)	101, 246, 509, 560
HpyF3I	CTNAG	8 cut(s)	96, 614, 839, 981, 1087, 1673, 1974, 2176
HpySE526I	ACGT	4 cut(s)	1332, 1425, 1735, 2071
Hsp92I	GRCGYC	1 cut(s)	108
HspAI	GCGC	5 cut(s)	108, 464, 1358, 1596, 1810
KasI	GGCGCC	1 cut(s)	107
Kpn2I	TCCGGA	1 cut(s)	2049
KroI	GCCGGC	1 cut(s)	104
KroNI	GCCGGC	1 cut(s)	106
Ksp22I	TGATCA	1 cut(s)	267
Kzo9I	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
LmnI	GCTCC	6 cut(s)	280, 1040, 1073, 1088, 1550, 1711
LweI	GCATC	6 cut(s)	91, 814, 1333, 1499, 1678, 1797
MaeI	CTAG	7 cut(s)	494, 758, 834, 951, 1301, 1458, 2200
MaeII	ACGT	4 cut(s)	1332, 1425, 1735, 2071
MaeIII	GTNAC	4 cut(s)	284, 1381, 2084, 2111
MalI	GATC	7 cut(s)	140, 269, 756, 847, 1258, 1420, 2064
MboI	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
MboII	GAAGA	8 cut(s)	79, 170, 349, 536, 626, 973, 1044, 2065
MfeI	CAATTG	1 cut(s)	289
MflI	RGATCY	4 cut(s)	754, 845, 1256, 1418
MhlI	GDGCHC	1 cut(s)	177
MlsI	TGGCCA	1 cut(s)	2038
MluNI	TGGCCA	1 cut(s)	2038
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	4 cut(s)	89, 114, 1873, 2190
MmeI	TCCRAC	3 cut(s)	258, 2058, 2173
Mox20I	TGGCCA	1 cut(s)	2038
Mph1103I	ATGCAT	1 cut(s)	196
MroI	TCCGGA	1 cut(s)	2049
MroNI	GCCGGC	1 cut(s)	104
MscI	TGGCCA	1 cut(s)	2038
MseI	TTAA	4 cut(s)	186, 930, 966, 1028
MslI	CAYNNNNRTG	3 cut(s)	199, 663, 1772
Msp20I	TGGCCA	1 cut(s)	2038
MspA1I	CMGCKG	3 cut(s)	503, 1753, 1824
MspI	CCGG	3 cut(s)	105, 1999, 2050
MspR9I	CCNGG	3 cut(s)	886, 1005, 1521
MunI	CAATTG	1 cut(s)	289
Mva1269I	GAATGC	2 cut(s)	206, 595
MvaI	CCWGG	3 cut(s)	886, 1005, 1521
MvnI	CGCG	2 cut(s)	1169, 2030
MwoI	GCNNNNNNNGC	4 cut(s)	101, 246, 509, 560
NaeI	GCCGGC	1 cut(s)	106
NarI	GGCGCC	1 cut(s)	108
NdeII	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
NgoMIV	GCCGGC	1 cut(s)	104
NlaIV	GGNNCC	5 cut(s)	109, 127, 891, 1728, 1831
NmeAIII	GCCGAG	2 cut(s)	580, 1921
NmuCI	GTSAC	2 cut(s)	2084, 2111
NsbI	TGCGCA	1 cut(s)	1811
NsiI	ATGCAT	1 cut(s)	196
NspI	RCATGY	2 cut(s)	682, 1114
OliI	CACNNNNGTG	1 cut(s)	663
PaeR7I	CTCGAG	1 cut(s)	170
PagI	TCATGA	1 cut(s)	324
PaqCI	CACCTGC	1 cut(s)	2138
PciI	ACATGT	1 cut(s)	678
PctI	GAATGC	2 cut(s)	206, 595
PdiI	GCCGGC	1 cut(s)	106
PfeI	GAWTC	3 cut(s)	458, 1849, 2105
Pfl23II	CGTACG	1 cut(s)	2068
PflFI	GACNNNGTC	1 cut(s)	123
PflMI	CCANNNNNTGG	2 cut(s)	412, 2192
PinAI	ACCGGT	1 cut(s)	1998
PleI	GAGTC	4 cut(s)	88, 114, 1873, 2190
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	4 cut(s)	88, 114, 1873, 2190
Ppu21I	YACGTR	1 cut(s)	2072
PpuMI	RGGWCCY	1 cut(s)	1636
PscI	ACATGT	1 cut(s)	678
Psp124BI	GAGCTC	1 cut(s)	177
Psp1406I	AACGTT	1 cut(s)	1425
Psp5II	RGGWCCY	1 cut(s)	1636
Psp6I	CCWGG	3 cut(s)	884, 1003, 1519
PspGI	CCWGG	3 cut(s)	884, 1003, 1519
PspLI	CGTACG	1 cut(s)	2068
PspN4I	GGNNCC	5 cut(s)	109, 127, 891, 1728, 1831
PspPI	GGNCC	4 cut(s)	125, 1001, 1636, 1829
PspPPI	RGGWCCY	1 cut(s)	1636
PspXI	VCTCGAGB	1 cut(s)	170
PstI	CTGCAG	1 cut(s)	1595
PstNI	CAGNNNCTG	1 cut(s)	1094
PsuI	RGATCY	4 cut(s)	754, 845, 1256, 1418
PsyI	GACNNNGTC	1 cut(s)	123
PvuII	CAGCTG	2 cut(s)	503, 1753
RsaI	GTAC	4 cut(s)	482, 683, 1922, 2070
RsaNI	GTAC	4 cut(s)	481, 682, 1921, 2069
RseI	CAYNNNNRTG	3 cut(s)	199, 663, 1772
SacI	GAGCTC	1 cut(s)	177
SaqAI	TTAA	4 cut(s)	186, 930, 966, 1028
Sau3AI	GATC	7 cut(s)	138, 267, 754, 845, 1256, 1418, 2062
Sau96I	GGNCC	4 cut(s)	125, 1001, 1636, 1829
ScaI	AGTACT	1 cut(s)	1922
SchI	GAGTC	4 cut(s)	89, 114, 1873, 2190
ScrFI	CCNGG	3 cut(s)	886, 1005, 1521
SduI	GDGCHC	1 cut(s)	177
SfaNI	GCATC	6 cut(s)	91, 814, 1333, 1499, 1678, 1797
SfcI	CTRYAG	4 cut(s)	1098, 1338, 1591, 1858
SfoI	GGCGCC	1 cut(s)	109
Sfr274I	CTCGAG	1 cut(s)	170
SinI	GGWCC	4 cut(s)	125, 1001, 1636, 1829
SlaI	CTCGAG	1 cut(s)	170
SmiMI	CAYNNNNRTG	3 cut(s)	199, 663, 1772
SmlI	CTYRAG	3 cut(s)	134, 170, 765
SmoI	CTYRAG	3 cut(s)	134, 170, 765
SsiI	CCGC	2 cut(s)	827, 1822
SspDI	GGCGCC	1 cut(s)	107
SspMI	CTAG	7 cut(s)	494, 758, 834, 951, 1301, 1458, 2200
SstI	GAGCTC	1 cut(s)	177
StyD4I	CCNGG	3 cut(s)	884, 1003, 1519
StyI	CCWWGG	2 cut(s)	1502, 1686
TaaI	ACNGT	8 cut(s)	184, 489, 1200, 1246, 1381, 1748, 1951, 2084
TaiI	ACGT	4 cut(s)	1335, 1428, 1738, 2074
TaqI	TCGA	6 cut(s)	54, 118, 171, 177, 1642, 1936
TaqII	GACCGA	2 cut(s)	113, 1783
TatI	WGTACW	2 cut(s)	681, 1920
TfiI	GAWTC	3 cut(s)	458, 1849, 2105
Tru1I	TTAA	4 cut(s)	186, 930, 966, 1028
Tru9I	TTAA	4 cut(s)	186, 930, 966, 1028
TscAI	CASTG	4 cut(s)	148, 187, 742, 1720
TseFI	GTSAC	2 cut(s)	2084, 2111
Tsp45I	GTSAC	2 cut(s)	2084, 2111
TspRI	CASTG	4 cut(s)	148, 187, 742, 1720
Tth111I	GACNNNGTC	1 cut(s)	123
Van91I	CCANNNNNTGG	2 cut(s)	412, 2192
VpaK11BI	GGWCC	4 cut(s)	125, 1001, 1636, 1829
XapI	RAATTY	2 cut(s)	1220, 1815
XbaI	TCTAGA	1 cut(s)	493
XceI	RCATGY	2 cut(s)	682, 1114
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1720
XhoI	CTCGAG	1 cut(s)	170
XmiI	GTMKAC	2 cut(s)	675, 1764
XspI	CTAG	7 cut(s)	494, 758, 834, 951, 1301, 1458, 2200
ZrmI	AGTACT	1 cut(s)	1922
Zsp2I	ATGCAT	1 cut(s)	196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.