MD14G1154600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016331
Physical Location & Seq
Reverse (-)
24916180 .. 24916935
756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1154600.v1.1.491

Sequence Viewer

Length: 756 bp
ATGAAGGAGGACCCCACCATGAAAGCTCACCAAGCAGAAGAAAAAGAAGAGAGCGTGGTTGCAAAACTGCAGAAGCAGTTCATGAGTCTGCAGCAAGACTGGGACTCTTACAAAAACTCAAACCCGAAAACCAAGAGGAGATATTCAACAAATTACTCATCAGGTTTTTCAATGGCGAAAAATCTTGACCAACTGCTTGATTCTTCCCCAAGGCATCTCATGTGTTCACTTCAGCATAGGGTTTCTCCATCAGAGGGAGGAGAGTGGAAGGTGAGGAGTAATGACTTGGCGGTGGAGGAGATACGGAGAGAGAGGAGGGCTGCCATTGAGAGCGGCAGGCTGAAGGGAAGGAGGCTTTTCGAGGCGGAGGATTGCGAAACCGAGAAGGGGTATGGAGGAAAAGAGGAGGGGACATGGTCGGGTGGTTGGAAAAATGGTTTGGCTCGAGAGGTGAGATCATTGTCTTTTGATGATGGTTCTGATGGTGATGATGATTCTGGTAAGAGCAAAGAGATTGGCGTTTGTTTTCAGTGGAGTGCGCAATCTTCGGGTTCTTTTTCTTTTTTGGCTGATGAGAATGTGAAGACAGAAGGCGTGAAAAAGGTTGATAGCAATGAAGGTGGTTATAATGGAGGAAGGTGCATTGTTATGGCGATGCGTGTGGTTGCTGTTGCTTCGATTGTGTTTGGAATTTGCTTTATCAGAGGCTTTGCTGGATATGGAAATGAAAACGAGGTGATCTTAGTCCCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

27.92

Weight (kDa)

5.81

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016331)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g08620
malus_domestica MD06G1138600.v1.1 MD14G1154600.v1.1
prunus_persica Prupe.5G148800_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0189791
rosa_laevigata RLG00000004590
rosa_multiflora Rmu_sc0014337.1_g000002 Rmu_sc0029387.1_g000002
rosa_roxburghii Rroxscaffold_3G00265160
rosa_rugosa Rorug06G0499900
rosa_samantha Rh7AG105000 Rh7BG107600 Rh7CG109600 Rh7DG108000
rosa_wichuraiana Rw7G009090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
Acc16I TGCGCA 1 cut(s) 540
AccBSI CCGCTC 1 cut(s) 333
AciI CCGC 3 cut(s) 290, 333, 365
AcsI RAATTY 1 cut(s) 690
AcuI CTGAAG 2 cut(s) 215, 362
AfiI CCNNNNNNNGG 2 cut(s) 254, 387
AgsI TTSAA 2 cut(s) 147, 171
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 91, 320
ApoI RAATTY 1 cut(s) 690
Asp700I GAANNNNTTC 1 cut(s) 77
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 5 cut(s) 20, 283, 463, 497, 748
AvaI CYCGRG 1 cut(s) 444
AvaII GGWCC 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 590
BbvI GCAGC 2 cut(s) 103, 307
BccI CCATC 3 cut(s) 256, 467, 476
BfmI CTRYAG 2 cut(s) 68, 89
BisI GCNGC 3 cut(s) 92, 321, 334
BlsI GCNGC 3 cut(s) 93, 322, 335
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 444
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 109
BmsI GCATC 2 cut(s) 223, 645
BmuI ACTGGG 1 cut(s) 109
BpiI GAAGAC 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 209
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 387
Bse1I ACTGG 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 619
BseDI CCNNGG 1 cut(s) 209
BseLI CCNNNNNNNGG 2 cut(s) 254, 387
BseMI GCAATG 1 cut(s) 619
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 6 cut(s) 151, 273, 289, 311, 328, 419
BseXI GCAGC 2 cut(s) 103, 307
BsiHKCI CYCGRG 1 cut(s) 444
BslFI GGGAC 3 cut(s) 116, 424, 731
BslI CCNNNNNNNGG 2 cut(s) 254, 387
BsmFI GGGAC 3 cut(s) 116, 424, 731
BsoBI CYCGRG 1 cut(s) 444
Bsp143I GATC 2 cut(s) 455, 738
BspACI CCGC 3 cut(s) 290, 333, 365
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 12
BspMAI CTGCAG 2 cut(s) 72, 93
BsrBI CCGCTC 1 cut(s) 333
BsrDI GCAATG 1 cut(s) 619
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 2 cut(s) 455, 738
BssT1I CCWWGG 1 cut(s) 209
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 1 cut(s) 742
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 2 cut(s) 458, 741
BstMBI GATC 2 cut(s) 455, 738
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstSFI CTRYAG 2 cut(s) 68, 89
BstV1I GCAGC 2 cut(s) 103, 307
BstV2I GAAGAC 1 cut(s) 590
BtgZI GCGATG 1 cut(s) 668
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 1 cut(s) 338
CciI TCATGA 1 cut(s) 81
CfoI GCGC 1 cut(s) 541
Cfr13I GGNCC 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 601, 636
CviAII CATG 5 cut(s) 19, 82, 220, 414, 753
CviJI RGCY 7 cut(s) 26, 320, 340, 355, 443, 569, 708
CviKI_1 RGCY 7 cut(s) 26, 320, 340, 355, 443, 569, 708
DdeI CTNAG 1 cut(s) 742
DpnI GATC 2 cut(s) 457, 740
DpnII GATC 2 cut(s) 455, 738
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
EciI GGCGGA 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 209
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 215, 362
Eco88I CYCGRG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 5 cut(s) 22, 85, 223, 417, 756
FaqI GGGAC 3 cut(s) 116, 424, 731
FatI CATG 5 cut(s) 18, 81, 219, 413, 752
Fnu4HI GCNGC 3 cut(s) 92, 321, 334
Fsp4HI GCNGC 3 cut(s) 92, 321, 334
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 1 cut(s) 540
GluI GCNGC 3 cut(s) 92, 321, 334
HhaI GCGC 1 cut(s) 541
Hin1II CATG 5 cut(s) 22, 85, 223, 417, 756
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HinfI GANTC 4 cut(s) 85, 104, 200, 494
HphI GGTGA 5 cut(s) 20, 283, 463, 497, 748
Hpy166II GTNNAC 1 cut(s) 227
Hpy188I TCNGA 3 cut(s) 253, 481, 704
Hpy188III TCNNGA 3 cut(s) 82, 185, 446
Hpy8I GTNNAC 1 cut(s) 227
HpyAV CCTTC 7 cut(s) 262, 337, 342, 379, 584, 611, 630
HpyCH4V TGCA 4 cut(s) 62, 70, 91, 642
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 1 cut(s) 742
Hsp92II CATG 5 cut(s) 22, 85, 223, 417, 756
HspAI GCGC 1 cut(s) 539
Kzo9I GATC 2 cut(s) 455, 738
LpnPI CCDG 5 cut(s) 85, 147, 322, 483, 699
Lsp1109I GCAGC 2 cut(s) 103, 307
LweI GCATC 2 cut(s) 223, 645
MalI GATC 2 cut(s) 457, 740
MbiI CCGCTC 1 cut(s) 333
MboI GATC 2 cut(s) 455, 738
MboII GAAGA 5 cut(s) 50, 59, 195, 537, 595
MluCI AATT 2 cut(s) 151, 690
MlyI GAGTC 2 cut(s) 94, 98
MmeI TCCRAC 1 cut(s) 407
MroXI GAANNNNTTC 1 cut(s) 77
MslI CAYNNNNRTG 1 cut(s) 647
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeII GATC 2 cut(s) 455, 738
NlaIII CATG 5 cut(s) 22, 85, 223, 417, 756
NlaIV GGNNCC 1 cut(s) 12
NsbI TGCGCA 1 cut(s) 540
PaeR7I CTCGAG 1 cut(s) 444
PagI TCATGA 1 cut(s) 81
PdmI GAANNNNTTC 1 cut(s) 77
PfeI GAWTC 2 cut(s) 200, 494
PflFI GACNNNGTC 1 cut(s) 415
PkrI GCNGC 3 cut(s) 93, 322, 335
PleI GAGTC 2 cut(s) 93, 98
PpsI GAGTC 2 cut(s) 93, 98
PpuMI RGGWCCY 1 cut(s) 10
PsiI TTATAA 1 cut(s) 627
Psp5II RGGWCCY 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 1 cut(s) 10
PspPPI RGGWCCY 1 cut(s) 10
PstI CTGCAG 2 cut(s) 72, 93
PsyI GACNNNGTC 1 cut(s) 415
RseI CAYNNNNRTG 1 cut(s) 647
SatI GCNGC 3 cut(s) 92, 321, 334
Sau3AI GATC 2 cut(s) 455, 738
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 2 cut(s) 94, 98
SetI ASST 8 cut(s) 28, 166, 273, 453, 606, 622, 641, 738
SfaNI GCATC 2 cut(s) 223, 645
SfcI CTRYAG 2 cut(s) 68, 89
Sfr274I CTCGAG 1 cut(s) 444
SinI GGWCC 1 cut(s) 10
SlaI CTCGAG 1 cut(s) 444
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 444
SmoI CTYRAG 1 cut(s) 444
Sse9I AATT 2 cut(s) 151, 690
SsiI CCGC 3 cut(s) 290, 333, 365
StyI CCWWGG 1 cut(s) 209
TaqI TCGA 3 cut(s) 360, 445, 677
TasI AATT 2 cut(s) 151, 690
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 2 cut(s) 200, 494
TscAI CASTG 1 cut(s) 536
TseI GCWGC 2 cut(s) 91, 320
TspDTI ATGAA 5 cut(s) 17, 35, 70, 630, 741
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 1 cut(s) 536
Tth111I GACNNNGTC 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 10
XapI RAATTY 1 cut(s) 690
XhoI CTCGAG 1 cut(s) 444
XmnI GAANNNNTTC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.