MD14G1169900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0019749
Physical Location & Seq
Forward (+)
26447595 .. 26450094
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1169900.v1.1.491

Sequence Viewer

Length: 303 bp
ATGAAGGAGAGGAGCAGGGAGATGCGCTCGAGTCCGCCAACGGCGGAAGAGATCTTCAGGAATTACAGTACTCGGAGAACCGACGTCGTCTACTCTTTAACTAACGCGTTCATCATCCCACACCAGGCGCCGCAGCCGCTCAAAGGCTGGGTCTCCAAAGGCGGTGTTTTGTTAAACCCTCCCCACTTAAGGTTTATAATCCCATTTAGCAGAGTCATGTCGCTTCCAAATGAAGAAAATGATAAGTCCTCTTCTTCTGATGCTCAGGTCCAATGGAGAACAATTCCCATCTTCCAGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.57

Weight (kDa)

10.25

Isoelectric Point (pI)

63.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019749)

Species Orthologous Gene IDs
malus_domestica MD03G1071200.v1.1 MD06G1018000.v1.1 MD08G1049100.v1.1 MD11G1248100.v1.1 MD14G1169900.v1.1
pyrus_communis pycom02g22110 pycom03g05060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
AatII GACGTC 1 cut(s) 87
AccB1I GGYRCC 1 cut(s) 127
AccBSI CCGCTC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 107
AciI CCGC 5 cut(s) 35, 44, 131, 137, 162
AcuI CTGAAG 1 cut(s) 40
AcyI GRCGYC 2 cut(s) 84, 128
AfaI GTAC 1 cut(s) 70
AfiI CCNNNNNNNGG 3 cut(s) 124, 143, 189
AflII CTTAAG 1 cut(s) 187
AflIII ACRYGT 1 cut(s) 105
AjnI CCWGG 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 28
ApeKI GCWGC 1 cut(s) 133
ArsI GACNNNNNNTTYG 2 cut(s) 135, 167
AspLEI GCGC 2 cut(s) 27, 130
AspS9I GGNCC 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 28
AvaII GGWCC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 145
BccI CCATC 1 cut(s) 296
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 131
BfrI CTTAAG 1 cut(s) 187
BglII AGATCT 1 cut(s) 51
BisI GCNGC 3 cut(s) 131, 134, 137
BlsI GCNGC 3 cut(s) 132, 135, 138
BmcAI AGTACT 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 12, 250
BplI GAGNNNNNCTC 2 cut(s) 11, 43
Bpu10I CCTNAGC 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 84, 128
BsaI GGTCTC 1 cut(s) 157
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 143, 189
BseBI CCWGG 1 cut(s) 125
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 3 cut(s) 124, 143, 189
BseMII CTCAG 1 cut(s) 278
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 107
BshNI GGYRCC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 28
BslI CCNNNNNNNGG 3 cut(s) 124, 143, 189
BsmAI GTCTC 1 cut(s) 157
Bso31I GGTCTC 1 cut(s) 157
BsoBI CYCGRG 1 cut(s) 28
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 5 cut(s) 35, 44, 131, 137, 162
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 127
BspTI CTTAAG 1 cut(s) 187
BspTNI GGTCTC 1 cut(s) 157
BsrBI CCGCTC 1 cut(s) 139
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 2 cut(s) 84, 128
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 68
Bst6I CTCTTC 2 cut(s) 42, 256
BstACI GRCGYC 2 cut(s) 84, 128
BstAFI CTTAAG 1 cut(s) 187
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 27, 130
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BtsCI GGATG 1 cut(s) 114
CfoI GCGC 2 cut(s) 27, 130
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 69
CviAII CATG 1 cut(s) 217
CviJI RGCY 2 cut(s) 136, 147
CviKI_1 RGCY 2 cut(s) 136, 147
CviQI GTAC 1 cut(s) 69
DdeI CTNAG 1 cut(s) 264
DinI GGCGCC 1 cut(s) 129
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 42, 256
EarI CTCTTC 2 cut(s) 42, 256
EciI GGCGGA 2 cut(s) 24, 59
Eco31I GGTCTC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 40
Eco88I CYCGRG 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 123
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
FaeI CATG 1 cut(s) 220
FaiI YATR 2 cut(s) 197, 218
FatI CATG 1 cut(s) 216
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 131, 134, 137
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 3 cut(s) 131, 134, 137
GlaI GCGC 2 cut(s) 26, 129
GluI GCNGC 3 cut(s) 131, 134, 137
GsaI CCCAGC 1 cut(s) 151
HaeII RGCGCY 1 cut(s) 131
HhaI GCGC 2 cut(s) 27, 130
Hin1I GRCGYC 2 cut(s) 84, 128
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 2 cut(s) 25, 128
HinP1I GCGC 2 cut(s) 25, 128
HinfI GANTC 2 cut(s) 31, 213
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 2 cut(s) 75, 259
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 2 cut(s) 86, 89
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 2 cut(s) 84, 128
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 2 cut(s) 25, 128
KasI GGCGCC 1 cut(s) 127
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 12
LpnPI CCDG 5 cut(s) 43, 110, 133, 137, 251
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 2 cut(s) 12, 250
MaeII ACGT 1 cut(s) 84
MalI GATC 1 cut(s) 53
MbiI CCGCTC 1 cut(s) 139
MboI GATC 1 cut(s) 51
MboII GAAGA 6 cut(s) 46, 59, 243, 245, 246, 283
MflI RGATCY 1 cut(s) 51
MluCI AATT 2 cut(s) 61, 282
MluI ACGCGT 1 cut(s) 105
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 2 cut(s) 40, 222
MnlI CCTC 3 cut(s) 3, 189, 259
MseI TTAA 3 cut(s) 98, 173, 188
MspCI CTTAAG 1 cut(s) 187
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 136
NarI GGCGCC 1 cut(s) 128
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 129
PaeR7I CTCGAG 1 cut(s) 28
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 3 cut(s) 132, 135, 138
PleI GAGTC 2 cut(s) 39, 221
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 2 cut(s) 39, 221
PsiI TTATAA 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 1 cut(s) 268
PspXI VCTCGAGB 1 cut(s) 28
PsuI RGATCY 1 cut(s) 51
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
SaqAI TTAA 3 cut(s) 98, 173, 188
SatI GCNGC 3 cut(s) 131, 134, 137
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 1 cut(s) 268
ScaI AGTACT 1 cut(s) 70
SchI GAGTC 2 cut(s) 40, 222
ScrFI CCNGG 1 cut(s) 125
SetI ASST 3 cut(s) 87, 194, 270
SfaNI GCATC 2 cut(s) 12, 250
SfoI GGCGCC 1 cut(s) 129
Sfr274I CTCGAG 1 cut(s) 28
SinI GGWCC 1 cut(s) 268
SlaI CTCGAG 1 cut(s) 28
SmlI CTYRAG 2 cut(s) 28, 187
SmoI CTYRAG 2 cut(s) 28, 187
Sse9I AATT 2 cut(s) 61, 282
SsiI CCGC 5 cut(s) 35, 44, 131, 137, 162
SspDI GGCGCC 1 cut(s) 127
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 29
TasI AATT 2 cut(s) 61, 282
TatI WGTACW 1 cut(s) 68
TauI GCSGC 2 cut(s) 133, 139
Tru1I TTAA 3 cut(s) 98, 173, 188
Tru9I TTAA 3 cut(s) 98, 173, 188
TseI GCWGC 1 cut(s) 133
TspDTI ATGAA 3 cut(s) 17, 100, 246
Tth111I GACNNNGTC 1 cut(s) 86
Vha464I CTTAAG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 90
ZraI GACGTC 1 cut(s) 85
ZrmI AGTACT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.