MD14G1171200.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007820
Physical Location & Seq
Reverse (-)
26518309 .. 26521587
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1171200.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGAGCGCTCCCAGTCCCATGGACGTCTCTATGCAAAACCCCCAAGACGTCGATGTCCCAGACCAAAACGGCGCCGTCGCAATAGACTCAGCTCCTTCTCCCATTCTTCCCCAAGACAAAGTCCTCGTTTCAGTGGAAGTCAGCCTCAAGCCCTCCAGCACAGCTCGGGCTGACGATGTGCGGTCTGCTGTAGAGAGAATGCTTGAAAAGAGGAGTCTGAGCTACACTGACGGACCTGTGCCGGTGCCGCTTGATGATCAGTTTCTGACAGAAAATGTGCAAAGAATCTGTATTTGTGACACGGATGAGTGGGTGCAAAACCATGACATCCTTTTGTTCTGGCAAGTGAAGCCTGTTGTGCATGTTTTTCAGCTTAGCGAAGAGGGAGCATGTGAGGATGTAAGTGGGGATGGACAACCTTCCACCTTCAACGAATGGATTCTTCCTGCAAATGAATTCGATGGCTTGTGGGAAAGTTTAATTTATGAATCAGGTCTCAAGCAAAGGTTGGTGCGGTATGCGGCTAGTGCATTGCTTTTCACTGAGAAGGGCGTCAATCCTTTCCTTGTGTCATGGAATCGCATTGTTCTTTTACATGGACCTCCAGGGACTGGAAAAACTTCTCTATGTAAAGCGCTGGCTCAAAAACTATCCATTCGGTTTAACTCCAGATACCCACAATGCCAGTTGATTGAAGTTAATGCACATTCTTTGTTCAGTAAATGGTTCTCTGAGAGTGGCAAGTTGGTTGCAAATCTTTTCTCAAAAATTCAGGAGATGGTAGAGGAGGAAAGCAATCTGGTATTTGTTTTGATTGATGAAGTTGAAAGCCTTGCTGCTGCTAGAAAAGCTGCTTTATCTGGCTCCGAACCTTCAGATTCTATTCGGGTAGTGAATGCGCTACTAACTCAGCTGGATAAATTGAAATCAGCACCAAATGTTATAATTCTGACAACATCCAACATAACTGCTGCTATTGATATTGCGTTTGTTGATCGAGCTGATATCAAAGCATATGTTGGTCCCCCAACTTTGCAAGCGCGTTATGAAATCCTAAGATCCTGCTTGCATGAGCTTATACGAACAGGAATTTTATCACATCTAGAGGATTGTGATAGCATGCTTCCTAACTTTGCTAGCTTGAGAGAGAAATTGAATATGCCTGAGGTACAAGATGCCCAGACACCACTACACCTGTGTAAACAATTGTTCGAAACTGCAGAAGCATGCGAGGGATTGAGTGGGCGATCCTTAAGAAAGCTTCCGTTCCTAGCACATGCGGCTCTTGCAAATCCTTACGGTTGCGACCCTAGCAAGTTCTTGAGTACAATGACAGATACGGCTAGGAGGGAGCGTTCTGAGCTACCTGACTGA

Protein Analysis

458

Amino Acids

50.52

Weight (kDa)

4.93

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRIP13_N PF23563 39 - 128 1.2e-29 TRIP13 N-terminal domain
AAA_16 PF13191 194 - 315 3.9e-08 AAA ATPase domain
AAA_22 PF13401 194 - 287 4.5e-07 AAA domain
AAA PF00004 196 - 341 4.3e-27 ATPase family associated with various cellular activities (AAA)
AAA_lid_TRIP13_C PF23242 344 - 451 1.6e-40 TRIP13-like, AAA+ ATPase lid C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 944
AasI GACNNNNNNGTC 1 cut(s) 53
AatII GACGTC 2 cut(s) 27, 51
AccB1I GGYRCC 2 cut(s) 71, 244
AccB7I CCANNNNNTGG 1 cut(s) 611
AccII CGCG 1 cut(s) 1042
AciI CCGC 5 cut(s) 181, 248, 514, 521, 1280
AclWI GGATC 2 cut(s) 1053, 1242
AcsI RAATTY 3 cut(s) 455, 768, 1089
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 4 cut(s) 24, 48, 72, 552
AfaI GTAC 2 cut(s) 1170, 1327
AfeI AGCGCT 2 cut(s) 7, 636
AfiI CCNNNNNNNGG 1 cut(s) 611
AflII CTTAAG 1 cut(s) 1252
AgsI TTSAA 6 cut(s) 206, 430, 695, 827, 925, 1156
AjnI CCWGG 1 cut(s) 604
AloI GAACNNNNNNTCC 2 cut(s) 1339, 1371
Alw26I GTCTC 2 cut(s) 31, 500
AlwI GGATC 2 cut(s) 1053, 1242
AlwNI CAGNNNCTG 2 cut(s) 265, 611
Ama87I CYCGRG 1 cut(s) 165
Aor51HI AGCGCT 2 cut(s) 7, 636
ApeKI GCWGC 4 cut(s) 836, 839, 851, 971
ApoI RAATTY 3 cut(s) 455, 768, 1089
Asp700I GAANNNNTTC 2 cut(s) 438, 619
AspLEI GCGC 5 cut(s) 8, 74, 637, 901, 1042
AspS9I GGNCC 3 cut(s) 233, 599, 1022
AsuII TTCGAA 1 cut(s) 1212
AsuNHI GCTAGC 1 cut(s) 1136
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 3 cut(s) 233, 599, 1022
AxyI CCTNAGG 1 cut(s) 1164
BanI GGYRCC 2 cut(s) 71, 244
BbvI GCAGC 4 cut(s) 823, 826, 838, 958
BccI CCATC 3 cut(s) 404, 455, 772
BceAI ACGGC 3 cut(s) 59, 85, 1356
BciT130I CCWGG 1 cut(s) 606
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 31, 500
BfaI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
BfmI CTRYAG 2 cut(s) 189, 1218
BfoI RGCGCY 3 cut(s) 9, 75, 638
BfrI CTTAAG 1 cut(s) 1252
BisI GCNGC 7 cut(s) 248, 522, 837, 840, 852, 972, 1281
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 7 cut(s) 249, 523, 838, 841, 853, 973, 1282
Bme1390I CCNGG 1 cut(s) 606
Bme18I GGWCC 3 cut(s) 233, 599, 1022
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 3 cut(s) 233, 599, 1022
BmiI GGNNCC 4 cut(s) 73, 246, 865, 1024
BmrFI CCNGG 1 cut(s) 606
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 1 cut(s) 1165
BmtI GCTAGC 1 cut(s) 1140
BmuI ACTGGG 1 cut(s) 6
BpmI CTGGAG 3 cut(s) 139, 588, 652
Bpu1102I GCTNAGC 1 cut(s) 374
Bpu14I TTCGAA 1 cut(s) 1212
BpuEI CTTGAG 4 cut(s) 131, 482, 1162, 1342
BsaHI GRCGYC 4 cut(s) 24, 48, 72, 552
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 2 cut(s) 18, 605
BsaXI ACNNNNNCTCC 2 cut(s) 1339, 1369
Bsc4I CCNNNNNNNGG 1 cut(s) 611
Bse118I RCCGGY 1 cut(s) 241
Bse1I ACTGG 3 cut(s) 12, 616, 685
Bse21I CCTNAGG 1 cut(s) 1164
Bse3DI GCAATG 1 cut(s) 530
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 18, 605
BseGI GGATG 5 cut(s) 310, 327, 403, 415, 956
BseLI CCNNNNNNNGG 1 cut(s) 611
BseMI GCAATG 1 cut(s) 530
BseMII CTCAG 7 cut(s) 102, 209, 534, 723, 923, 1155, 1350
BseNI ACTGG 3 cut(s) 12, 616, 685
BseRI GAGGAG 2 cut(s) 226, 800
BseXI GCAGC 4 cut(s) 823, 826, 838, 958
Bsh1236I CGCG 1 cut(s) 1042
BshNI GGYRCC 2 cut(s) 71, 244
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 3 cut(s) 41, 622, 1008
BslI CCNNNNNNNGG 1 cut(s) 611
BsmAI GTCTC 2 cut(s) 31, 500
BsmBI CGTCTC 1 cut(s) 31
BsmFI GGGAC 3 cut(s) 41, 622, 1008
BsmI GAATGC 2 cut(s) 204, 901
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 1 cut(s) 165
Bsp119I TTCGAA 1 cut(s) 1212
Bsp143I GATC 4 cut(s) 256, 994, 1058, 1247
Bsp1720I GCTNAGC 1 cut(s) 374
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 5 cut(s) 181, 248, 514, 521, 1280
BspCNI CTCAG 7 cut(s) 101, 210, 535, 724, 922, 1156, 1351
BspFNI CGCG 1 cut(s) 1042
BspLI GGNNCC 4 cut(s) 73, 246, 865, 1024
BspMAI CTGCAG 1 cut(s) 1222
BspOI GCTAGC 1 cut(s) 1140
BspPI GGATC 2 cut(s) 1053, 1242
BspT104I TTCGAA 1 cut(s) 1212
BspT107I GGYRCC 2 cut(s) 71, 244
BspTI CTTAAG 1 cut(s) 1252
BspTNI GGTCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 530
BsrFI RCCGGY 1 cut(s) 241
BsrI ACTGG 3 cut(s) 12, 616, 685
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 18, 605
BssMI GATC 4 cut(s) 256, 994, 1058, 1247
BssNI GRCGYC 4 cut(s) 24, 48, 72, 552
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 1 cut(s) 1301
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 4 cut(s) 24, 48, 72, 552
BstAFI CTTAAG 1 cut(s) 1252
BstBI TTCGAA 1 cut(s) 1212
BstC8I GCNNGC 6 cut(s) 639, 1038, 1067, 1121, 1138, 1228
BstDEI CTNAG 9 cut(s) 88, 218, 374, 543, 732, 909, 1055, 1164, 1359
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 5 cut(s) 310, 327, 403, 415, 956
BstFNI CGCG 1 cut(s) 1042
BstH2I RGCGCY 3 cut(s) 9, 75, 638
BstHHI GCGC 5 cut(s) 8, 74, 637, 901, 1042
BstKTI GATC 4 cut(s) 259, 997, 1061, 1250
BstMAI GTCTC 2 cut(s) 31, 500
BstMBI GATC 4 cut(s) 256, 994, 1058, 1247
BstMWI GCNNNNNNNGC 9 cut(s) 247, 349, 358, 527, 848, 1280, 1286, 1311, 1360
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 5 cut(s) 365, 393, 1123, 1230, 1280
BstSCI CCNGG 1 cut(s) 604
BstSFI CTRYAG 2 cut(s) 189, 1218
BstUI CGCG 1 cut(s) 1042
BstV1I GCAGC 4 cut(s) 823, 826, 838, 958
BstX2I RGATCY 1 cut(s) 1058
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 1058
Bsu36I CCTNAGG 1 cut(s) 1164
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 5 cut(s) 310, 327, 403, 415, 956
BtsIMutI CAGTG 3 cut(s) 138, 225, 540
Cac8I GCNNGC 6 cut(s) 639, 1038, 1067, 1121, 1138, 1228
CaiI CAGNNNCTG 2 cut(s) 265, 611
CfoI GCGC 5 cut(s) 8, 74, 637, 901, 1042
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 3 cut(s) 233, 599, 1022
CseI GACGC 1 cut(s) 541
Csp6I GTAC 2 cut(s) 1169, 1326
CviQI GTAC 2 cut(s) 1169, 1326
DdeI CTNAG 9 cut(s) 88, 218, 374, 543, 732, 909, 1055, 1164, 1359
DinI GGCGCC 1 cut(s) 73
DpnI GATC 4 cut(s) 258, 996, 1060, 1249
DpnII GATC 4 cut(s) 256, 994, 1058, 1247
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 500
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 3 cut(s) 233, 599, 1022
Eco47III AGCGCT 2 cut(s) 7, 636
Eco57I CTGAAG 1 cut(s) 858
Eco81I CCTNAGG 1 cut(s) 1164
Eco88I CYCGRG 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 604
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 18
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 31
FaqI GGGAC 3 cut(s) 41, 622, 1008
FauNDI CATATG 1 cut(s) 1015
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 7 cut(s) 248, 522, 837, 840, 852, 972, 1281
FokI GGATG 5 cut(s) 314, 317, 410, 422, 943
Fsp4HI GCNGC 7 cut(s) 248, 522, 837, 840, 852, 972, 1281
FspBI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
GlaI GCGC 5 cut(s) 7, 73, 636, 900, 1041
GluI GCNGC 7 cut(s) 248, 522, 837, 840, 852, 972, 1281
GsuI CTGGAG 3 cut(s) 139, 588, 652
HaeII RGCGCY 3 cut(s) 9, 75, 638
HapII CCGG 1 cut(s) 242
HgaI GACGC 1 cut(s) 541
HhaI GCGC 5 cut(s) 8, 74, 637, 901, 1042
Hin1I GRCGYC 4 cut(s) 24, 48, 72, 552
Hin6I GCGC 5 cut(s) 6, 72, 635, 899, 1040
HinP1I GCGC 5 cut(s) 6, 72, 635, 899, 1040
HindIII AAGCTT 1 cut(s) 1259
HinfI GANTC 7 cut(s) 86, 214, 285, 439, 488, 577, 878
HpaII CCGG 1 cut(s) 242
Hpy166II GTNNAC 1 cut(s) 1202
Hpy188I TCNGA 7 cut(s) 219, 267, 733, 868, 877, 951, 1360
Hpy188III TCNNGA 4 cut(s) 669, 773, 1103, 1321
Hpy8I GTNNAC 1 cut(s) 1202
Hpy99I CGWCG 2 cut(s) 53, 80
HpyAV CCTTC 5 cut(s) 105, 429, 436, 541, 882
HpyCH4III ACNGT 1 cut(s) 1301
HpyCH4IV ACGT 2 cut(s) 24, 48
HpyF10VI GCNNNNNNNGC 9 cut(s) 247, 349, 358, 527, 848, 1280, 1286, 1311, 1360
HpyF3I CTNAG 9 cut(s) 88, 218, 374, 543, 732, 909, 1055, 1164, 1359
HpySE526I ACGT 2 cut(s) 24, 48
Hsp92I GRCGYC 4 cut(s) 24, 48, 72, 552
HspAI GCGC 5 cut(s) 6, 72, 635, 899, 1040
KasI GGCGCC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 4 cut(s) 256, 994, 1058, 1247
LmnI GCTCC 5 cut(s) 13, 97, 386, 869, 1351
Lsp1109I GCAGC 4 cut(s) 823, 826, 838, 958
LweI GCATC 1 cut(s) 1165
MaeI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
MaeII ACGT 2 cut(s) 24, 48
MaeIII GTNAC 1 cut(s) 296
MalI GATC 4 cut(s) 258, 996, 1060, 1249
MboI GATC 4 cut(s) 256, 994, 1058, 1247
MboII GAAGA 3 cut(s) 98, 392, 434
MfeI CAATTG 1 cut(s) 1205
MflI RGATCY 1 cut(s) 1058
MluCI AATT 8 cut(s) 455, 480, 768, 920, 945, 1089, 1151, 1205
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 2 cut(s) 80, 223
MmeI TCCRAC 1 cut(s) 984
MroXI GAANNNNTTC 2 cut(s) 438, 619
MseI TTAA 4 cut(s) 479, 663, 699, 1253
MspA1I CMGCKG 1 cut(s) 913
MspCI CTTAAG 1 cut(s) 1252
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 1 cut(s) 606
MunI CAATTG 1 cut(s) 1205
Mva1269I GAATGC 2 cut(s) 204, 901
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 1 cut(s) 1042
MwoI GCNNNNNNNGC 9 cut(s) 247, 349, 358, 527, 848, 1280, 1286, 1311, 1360
NarI GGCGCC 1 cut(s) 72
NcoI CCATGG 1 cut(s) 18
NdeI CATATG 1 cut(s) 1015
NdeII GATC 4 cut(s) 256, 994, 1058, 1247
NheI GCTAGC 1 cut(s) 1136
NlaIV GGNNCC 4 cut(s) 73, 246, 865, 1024
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 5 cut(s) 365, 393, 1123, 1230, 1280
NspV TTCGAA 1 cut(s) 1212
PaeI GCATGC 2 cut(s) 1123, 1230
PctI GAATGC 2 cut(s) 204, 901
PdmI GAANNNNTTC 2 cut(s) 438, 619
PfeI GAWTC 5 cut(s) 285, 439, 488, 577, 878
PflFI GACNNNGTC 1 cut(s) 119
PflMI CCANNNNNTGG 1 cut(s) 611
PkrI GCNGC 7 cut(s) 249, 523, 838, 841, 853, 973, 1282
PleI GAGTC 2 cut(s) 80, 222
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 2 cut(s) 80, 222
PsiI TTATAA 1 cut(s) 944
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 4 cut(s) 73, 246, 865, 1024
PspPI GGNCC 3 cut(s) 233, 599, 1022
PstI CTGCAG 1 cut(s) 1222
PstNI CAGNNNCTG 2 cut(s) 265, 611
PsuI RGATCY 1 cut(s) 1058
PsyI GACNNNGTC 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 913
RsaI GTAC 2 cut(s) 1170, 1327
RsaNI GTAC 2 cut(s) 1169, 1326
SaqAI TTAA 4 cut(s) 479, 663, 699, 1253
SatI GCNGC 7 cut(s) 248, 522, 837, 840, 852, 972, 1281
Sau3AI GATC 4 cut(s) 256, 994, 1058, 1247
Sau96I GGNCC 3 cut(s) 233, 599, 1022
SchI GAGTC 2 cut(s) 80, 223
ScrFI CCNGG 1 cut(s) 606
SfaNI GCATC 1 cut(s) 1165
SfcI CTRYAG 2 cut(s) 189, 1218
SfoI GGCGCC 1 cut(s) 73
SfuI TTCGAA 1 cut(s) 1212
SinI GGWCC 3 cut(s) 233, 599, 1022
SmlI CTYRAG 5 cut(s) 146, 497, 1141, 1252, 1321
SmoI CTYRAG 5 cut(s) 146, 497, 1141, 1252, 1321
SphI GCATGC 2 cut(s) 1123, 1230
Sse9I AATT 8 cut(s) 455, 480, 768, 920, 945, 1089, 1151, 1205
SsiI CCGC 5 cut(s) 181, 248, 514, 521, 1280
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 1 cut(s) 1301
TaiI ACGT 2 cut(s) 27, 51
TaqI TCGA 4 cut(s) 51, 459, 997, 1212
TasI AATT 8 cut(s) 455, 480, 768, 920, 945, 1089, 1151, 1205
TatI WGTACW 1 cut(s) 1325
TauI GCSGC 3 cut(s) 250, 524, 1283
TfiI GAWTC 5 cut(s) 285, 439, 488, 577, 878
Tru1I TTAA 4 cut(s) 479, 663, 699, 1253
Tru9I TTAA 4 cut(s) 479, 663, 699, 1253
TscAI CASTG 3 cut(s) 138, 232, 547
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 4 cut(s) 836, 839, 851, 971
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 4 cut(s) 468, 501, 834, 1062
TspGWI ACGGA 3 cut(s) 246, 317, 1254
TspRI CASTG 3 cut(s) 138, 232, 547
Tth111I GACNNNGTC 1 cut(s) 119
Van91I CCANNNNNTGG 1 cut(s) 611
Vha464I CTTAAG 1 cut(s) 1252
VpaK11BI GGWCC 3 cut(s) 233, 599, 1022
XapI RAATTY 3 cut(s) 455, 768, 1089
XbaI TCTAGA 1 cut(s) 1102
XceI RCATGY 5 cut(s) 365, 393, 1123, 1230, 1280
XmnI GAANNNNTTC 2 cut(s) 438, 619
XspI CTAG 7 cut(s) 525, 843, 1103, 1137, 1271, 1311, 1344
ZraI GACGTC 2 cut(s) 25, 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.