MD14G1201600.v1.1

Cyclic dof factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013861
Physical Location & Seq
Forward (+)
29043548 .. 29046646
3099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1201600.v1.1.491

Sequence Viewer

Length: 1401 bp
ATGCTCGAAACTAAGGACCCCGCGATCAAGCTCTTCGGGAAGAAGATTTCCCTCCCCGCCGACATCGAGGACCTGGGTTTTGCTCGCCCTGTGGAGAAAGAAGTGGAAGTAGAATTGGAAGAGGAGTGCGAAGAGGAGGAAGAAGATGCAGAGACTGAGCAGGATTCGCCGTCACAAAAATCGACTGAGAGCATAGAACAAGATGATGGTTCTCAACCAAAATCTGAAGACCCTACAAATCTGGAAACATTACCAGGTTCTGATGTGAACCCGAAGACACCCTCTATAGATGAAGAAAGTGCACAAACAAAAACTGCTCAGACGGAGAAAGAACAGAGTACTGAAAACTCGGAAGAGAAAACCCTGAAGAAGCCTGACAAGATACTTCCATGCCCCCGCTGCAATAGCATGGACACTAAATTTTGTTACTACAACAATTACAACGTGAATCAACCACGCCATTTCTGTAAAGCCTGTCAAAGATACTGGACAGCAGGCGGTACCATGAGGAATGTGCCTGTGGGAGCTGGACGTCGCAAAAACAAGAACTCTGCCTCACACTATCGCCACATGACCATTAGCGAGGCTCTTGAAGCAGCTCGGATTGATGCTCCAAATGGAGTTCACCACCCTGCATTAAAAAGCAATGGTAGAGTCCTCAGCTTCGGAGTGGATGCACCTATTTGTGATTCTATGGCTTCTTCTCTAAATCCTGCAGATAAAAAGGTTCCAAATGGTACTCGAAATGGTTTACATAAAAGCGAAGAACAGGTATTTCCATCTCCTTGCAAAGCTAGGGACAATGGCGATGATAGCTCCAGTGCATCTTCTGTAAATGTTTCAAAACCACATGTGCAGAATGTCAATGGCTTCTCTTCGCAAATTCCTTGTCTTCCCGGTGTTCCCTGGCCTTATCCATGGAATGCAGCAGTTCCCCCACCGCCTTTTTGTCCTCCAGGATTTCCTCTATCATTTTGTCCTGCACCTTATTGGAACTGTGGTGTGCCCGGCACTTGGAACGTTCCTTGGTTTGGTCCACAACCCGCTTCTCCAAACCAAAAGTCTCCCCGCTCTGGTTCAAATTCTCCAACATTGGGGAAGCACTCAAGGGACGAAGACGTTCTAAAACCAGAAATTTTGGAGAAAGAAGAGCCATCAAGACAGAAAAATGGATGTGTTTTGGTTCCAAAAACATTAAGGATTGATGATCCGAGGGAAGCTGCAAAGAGTTCAATATGGGCAACGCTCGGAATCAAGAACGATTCCATCAGTGGCGGAGGGTTGTTTAAGGCTTTCCAGTCAAAGAGTGACAAAAAGAAAACCGTTTCTGAATCCTCTCCCGCTTTGCGAGCAAATCCAGCTGCGTTGTCTAGATCCCTCAACTTTCATGAGAGCTCATGA

Protein Analysis

467

Amino Acids

50.98

Weight (kDa)

5.75

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 127 - 183 4.9e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013861)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47500
fragaria_vesca FvH4_5g14830
malus_domestica MD06G1194500.v1.1 MD14G1201600.v1.1
prunus_persica Prupe.5G194600_v2.0.a1
pyrus_communis pycom06g17220 pycom14g16700
rosa_chinensis RchiOBHm_Chr7g0181561
rosa_laevigata RLG00000005183
rosa_multiflora Rmu_sc0000988.1_g000022
rosa_roxburghii Rroxscaffold_3G00271900
rosa_rugosa Rorug06G0442200
rosa_samantha Rh7AG044400 Rh7BG043400 Rh7CG045200 Rh7DG044000
rosa_wichuraiana Rw7G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 535
Acc65I GGTACC 1 cut(s) 500
AccB1I GGYRCC 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 1071
AccII CGCG 1 cut(s) 23
AciI CCGC 9 cut(s) 21, 57, 397, 498, 941, 1044, 1069, 1275, 1341
AclI AACGTT 1 cut(s) 1020
AclWI GGATC 2 cut(s) 1202, 1368
AcsI RAATTY 4 cut(s) 419, 882, 1081, 1134
AcuI CTGAAG 2 cut(s) 246, 386
AcyI GRCGYC 1 cut(s) 532
AfaI GTAC 3 cut(s) 340, 502, 739
AfiI CCNNNNNNNGG 4 cut(s) 1014, 1031, 1073, 1094
AflIII ACRYGT 1 cut(s) 850
AgsI TTSAA 4 cut(s) 593, 843, 1080, 1233
AjnI CCWGG 4 cut(s) 72, 253, 905, 955
AluBI AGCT 9 cut(s) 31, 527, 599, 663, 794, 816, 1220, 1361, 1395
AluI AGCT 9 cut(s) 31, 527, 599, 663, 794, 816, 1220, 1361, 1395
Alw21I GWGCWC 2 cut(s) 304, 1397
Alw26I GTCTC 2 cut(s) 146, 1068
Alw44I GTGCAC 1 cut(s) 300
AlwI GGATC 2 cut(s) 1202, 1368
AlwNI CAGNNNCTG 2 cut(s) 155, 260
AoxI GGCC 1 cut(s) 908
ApaLI GTGCAC 1 cut(s) 300
ApeKI GCWGC 5 cut(s) 399, 596, 926, 1220, 1361
ApoI RAATTY 4 cut(s) 419, 882, 1081, 1134
ArsI GACNNNNNNTTYG 6 cut(s) 62, 94, 874, 906, 1046, 1078
Asp700I GAANNNNTTC 1 cut(s) 1119
Asp718I GGTACC 1 cut(s) 500
AspS9I GGNCC 3 cut(s) 16, 70, 1034
AsuC2I CCSGG 2 cut(s) 897, 1008
AsuHPI GGTGA 1 cut(s) 617
AvaII GGWCC 3 cut(s) 16, 70, 1034
BaeGI GKGCMC 2 cut(s) 304, 1008
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 1397
BbsI GAAGAC 4 cut(s) 234, 281, 884, 1122
Bbv12I GWGCWC 2 cut(s) 304, 1397
BbvCI CCTCAGC 1 cut(s) 659
BbvI GCAGC 5 cut(s) 386, 608, 938, 1207, 1348
BccI CCATC 4 cut(s) 200, 787, 1162, 1274
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 4 cut(s) 74, 255, 907, 957
BcnI CCSGG 2 cut(s) 897, 1008
BcoDI GTCTC 2 cut(s) 146, 1068
BfaI CTAG 2 cut(s) 795, 1371
BfmI CTRYAG 2 cut(s) 285, 714
BisI GCNGC 5 cut(s) 400, 597, 927, 1221, 1362
BlsI GCNGC 5 cut(s) 401, 598, 928, 1222, 1363
BmcAI AGTACT 1 cut(s) 340
Bme1390I CCNGG 6 cut(s) 74, 255, 897, 907, 957, 1008
Bme18I GGWCC 3 cut(s) 16, 70, 1034
BmgT120I GGNCC 3 cut(s) 16, 70, 1034
BmiI GGNNCC 4 cut(s) 18, 502, 729, 1185
BmrFI CCNGG 6 cut(s) 74, 255, 897, 907, 957, 1008
BmsI GCATC 4 cut(s) 136, 598, 664, 833
BpiI GAAGAC 4 cut(s) 234, 281, 884, 1122
BpmI CTGGAG 2 cut(s) 802, 939
Bpu10I CCTNAGC 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 1090
BpuMI CCSGG 2 cut(s) 897, 1008
BsaHI GRCGYC 1 cut(s) 532
BsaJI CCNNGG 5 cut(s) 73, 905, 917, 1025, 1211
Bsc4I CCNNNNNNNGG 4 cut(s) 1014, 1031, 1073, 1094
Bse1I ACTGG 3 cut(s) 491, 819, 1297
Bse3DI GCAATG 1 cut(s) 652
BseBI CCWGG 4 cut(s) 74, 255, 907, 957
BseDI CCNNGG 5 cut(s) 73, 905, 917, 1025, 1211
BseGI GGATG 2 cut(s) 679, 1178
BseLI CCNNNNNNNGG 4 cut(s) 1014, 1031, 1073, 1094
BseMI GCAATG 1 cut(s) 652
BseMII CTCAG 4 cut(s) 147, 177, 332, 673
BseNI ACTGG 3 cut(s) 491, 819, 1297
BseRI GAGGAG 2 cut(s) 137, 149
BseSI GKGCMC 2 cut(s) 304, 1008
BseXI GCAGC 5 cut(s) 386, 608, 938, 1207, 1348
BsgI GTGCAG 2 cut(s) 875, 966
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 1 cut(s) 910
BshNI GGYRCC 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 304, 1397
BsiSI CCGG 2 cut(s) 897, 1008
BslFI GGGAC 2 cut(s) 812, 1124
BslI CCNNNNNNNGG 4 cut(s) 1014, 1031, 1073, 1094
BsmAI GTCTC 2 cut(s) 146, 1068
BsmFI GGGAC 2 cut(s) 812, 1124
BsmI GAATGC 1 cut(s) 928
BsnI GGCC 1 cut(s) 910
Bsp1286I GDGCHC 3 cut(s) 304, 1008, 1397
Bsp143I GATC 3 cut(s) 24, 1207, 1373
Bsp19I CCATGG 1 cut(s) 917
BspACI CCGC 9 cut(s) 21, 57, 397, 498, 941, 1044, 1069, 1275, 1341
BspANI GGCC 1 cut(s) 910
BspCNI CTCAG 4 cut(s) 148, 178, 331, 672
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 2 cut(s) 1387, 1397
BspLI GGNNCC 4 cut(s) 18, 502, 729, 1185
BspMAI CTGCAG 1 cut(s) 718
BspPI GGATC 2 cut(s) 1202, 1368
BspQI GCTCTTC 2 cut(s) 38, 1143
BspT107I GGYRCC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 1071
BsrDI GCAATG 1 cut(s) 652
BsrI ACTGG 3 cut(s) 491, 819, 1297
BssECI CCNNGG 5 cut(s) 73, 905, 917, 1025, 1211
BssMI GATC 3 cut(s) 24, 1207, 1373
BssNI GRCGYC 1 cut(s) 532
BssT1I CCWWGG 2 cut(s) 917, 1025
Bst2UI CCWGG 4 cut(s) 74, 255, 907, 957
Bst4CI ACNGT 2 cut(s) 998, 1324
Bst6I CTCTTC 6 cut(s) 38, 114, 126, 348, 880, 1143
BstACI GRCGYC 1 cut(s) 532
BstC8I GCNNGC 3 cut(s) 85, 496, 1350
BstDEI CTNAG 5 cut(s) 12, 156, 186, 318, 659
BstDSI CCRYGG 1 cut(s) 917
BstF5I GGATG 2 cut(s) 679, 1178
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 3 cut(s) 27, 1210, 1376
BstMAI GTCTC 2 cut(s) 146, 1068
BstMBI GATC 3 cut(s) 24, 1207, 1373
BstMWI GCNNNNNNNGC 7 cut(s) 166, 399, 405, 593, 813, 1349, 1358
BstNI CCWGG 4 cut(s) 74, 255, 907, 957
BstNSI RCATGY 1 cut(s) 854
BstSCI CCNGG 6 cut(s) 72, 253, 895, 905, 955, 1006
BstSFI CTRYAG 2 cut(s) 285, 714
BstSLI GKGCMC 2 cut(s) 304, 1008
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 5 cut(s) 386, 608, 938, 1207, 1348
BstV2I GAAGAC 4 cut(s) 234, 281, 884, 1122
BstX2I RGATCY 1 cut(s) 1373
BstYI RGATCY 1 cut(s) 1373
BsuRI GGCC 1 cut(s) 910
BtgI CCRYGG 1 cut(s) 917
BtgZI GCGATG 1 cut(s) 822
BtsCI GGATG 2 cut(s) 679, 1178
BtsIMutI CAGTG 2 cut(s) 826, 1276
Cac8I GCNNGC 3 cut(s) 85, 496, 1350
CaiI CAGNNNCTG 2 cut(s) 155, 260
CciI TCATGA 2 cut(s) 1387, 1397
Cfr13I GGNCC 3 cut(s) 16, 70, 1034
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 3 cut(s) 339, 501, 738
CviAII CATG 8 cut(s) 390, 409, 505, 571, 851, 918, 1388, 1398
CviQI GTAC 3 cut(s) 339, 501, 738
DdeI CTNAG 5 cut(s) 12, 156, 186, 318, 659
DpnI GATC 3 cut(s) 26, 1209, 1375
DpnII GATC 3 cut(s) 24, 1207, 1373
Eam1104I CTCTTC 6 cut(s) 38, 114, 126, 348, 880, 1143
EarI CTCTTC 6 cut(s) 38, 114, 126, 348, 880, 1143
EciI GGCGGA 1 cut(s) 1290
Ecl136II GAGCTC 1 cut(s) 1395
Eco130I CCWWGG 2 cut(s) 917, 1025
Eco24I GRGCYC 1 cut(s) 1397
Eco47I GGWCC 3 cut(s) 16, 70, 1034
Eco53kI GAGCTC 1 cut(s) 1395
Eco57I CTGAAG 2 cut(s) 246, 386
EcoICRI GAGCTC 1 cut(s) 1395
EcoO109I RGGNCCY 2 cut(s) 16, 70
EcoRII CCWGG 4 cut(s) 72, 253, 905, 955
EcoT14I CCWWGG 2 cut(s) 917, 1025
EcoT38I GRGCYC 1 cut(s) 1397
ErhI CCWWGG 2 cut(s) 917, 1025
FaeI CATG 8 cut(s) 393, 412, 508, 574, 854, 921, 1391, 1401
FaqI GGGAC 2 cut(s) 812, 1124
FatI CATG 8 cut(s) 389, 408, 504, 570, 850, 917, 1387, 1397
FauI CCCGC 6 cut(s) 28, 64, 404, 1051, 1076, 1348
Fnu4HI GCNGC 5 cut(s) 400, 597, 927, 1221, 1362
FokI GGATG 2 cut(s) 686, 1185
FriOI GRGCYC 1 cut(s) 1397
Fsp4HI GCNGC 5 cut(s) 400, 597, 927, 1221, 1362
FspBI CTAG 2 cut(s) 795, 1371
GluI GCNGC 5 cut(s) 400, 597, 927, 1221, 1362
GsuI CTGGAG 2 cut(s) 802, 939
HaeIII GGCC 1 cut(s) 910
HapII CCGG 2 cut(s) 897, 1008
Hin1I GRCGYC 1 cut(s) 532
Hin1II CATG 8 cut(s) 393, 412, 508, 574, 854, 921, 1391, 1401
HinfI GANTC 7 cut(s) 164, 448, 654, 689, 1251, 1262, 1331
HpaII CCGG 2 cut(s) 897, 1008
HphI GGTGA 1 cut(s) 617
Hpy166II GTNNAC 5 cut(s) 268, 302, 625, 752, 1037
Hpy188I TCNGA 9 cut(s) 226, 262, 321, 352, 603, 668, 1212, 1250, 1330
Hpy188III TCNNGA 8 cut(s) 37, 242, 590, 1158, 1255, 1371, 1388, 1398
Hpy8I GTNNAC 5 cut(s) 268, 302, 625, 752, 1037
Hpy99I CGWCG 1 cut(s) 537
HpyCH4III ACNGT 2 cut(s) 998, 1324
HpyCH4IV ACGT 4 cut(s) 444, 532, 1020, 1119
HpyF10VI GCNNNNNNNGC 7 cut(s) 166, 399, 405, 593, 813, 1349, 1358
HpyF3I CTNAG 5 cut(s) 12, 156, 186, 318, 659
HpySE526I ACGT 4 cut(s) 444, 532, 1020, 1119
Hsp92I GRCGYC 1 cut(s) 532
Hsp92II CATG 8 cut(s) 393, 412, 508, 574, 854, 921, 1391, 1401
KpnI GGTACC 1 cut(s) 504
Kzo9I GATC 3 cut(s) 24, 1207, 1373
LguI GCTCTTC 2 cut(s) 38, 1143
LmnI GCTCC 3 cut(s) 524, 616, 821
Lsp1109I GCAGC 5 cut(s) 386, 608, 938, 1207, 1348
LweI GCATC 4 cut(s) 136, 598, 664, 833
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 2 cut(s) 795, 1371
MaeII ACGT 4 cut(s) 444, 532, 1020, 1119
MaeIII GTNAC 3 cut(s) 171, 425, 1307
MalI GATC 3 cut(s) 26, 1209, 1375
MbiI CCGCTC 1 cut(s) 1071
MboI GATC 3 cut(s) 24, 1207, 1373
MflI RGATCY 1 cut(s) 1373
MhlI GDGCHC 3 cut(s) 304, 1008, 1397
MluCI AATT 6 cut(s) 113, 419, 436, 882, 1081, 1134
MlyI GAGTC 1 cut(s) 663
MmeI TCCRAC 1 cut(s) 1112
MroXI GAANNNNTTC 1 cut(s) 1119
MseI TTAA 3 cut(s) 638, 1196, 1287
MspA1I CMGCKG 2 cut(s) 399, 1361
MspI CCGG 2 cut(s) 897, 1008
MspR9I CCNGG 6 cut(s) 74, 255, 897, 907, 957, 1008
Mva1269I GAATGC 1 cut(s) 928
MvaI CCWGG 4 cut(s) 74, 255, 907, 957
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 7 cut(s) 166, 399, 405, 593, 813, 1349, 1358
NciI CCSGG 2 cut(s) 897, 1008
NcoI CCATGG 1 cut(s) 917
NdeII GATC 3 cut(s) 24, 1207, 1373
NlaIII CATG 8 cut(s) 393, 412, 508, 574, 854, 921, 1391, 1401
NlaIV GGNNCC 4 cut(s) 18, 502, 729, 1185
NmuCI GTSAC 2 cut(s) 171, 1307
NspI RCATGY 1 cut(s) 854
PagI TCATGA 2 cut(s) 1387, 1397
PciI ACATGT 1 cut(s) 850
PciSI GCTCTTC 2 cut(s) 38, 1143
PctI GAATGC 1 cut(s) 928
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 6 cut(s) 164, 448, 689, 1251, 1262, 1331
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 5 cut(s) 401, 598, 928, 1222, 1363
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
PpuMI RGGWCCY 2 cut(s) 16, 70
PscI ACATGT 1 cut(s) 850
Psp124BI GAGCTC 1 cut(s) 1397
Psp1406I AACGTT 1 cut(s) 1020
Psp5II RGGWCCY 2 cut(s) 16, 70
Psp6I CCWGG 4 cut(s) 72, 253, 905, 955
PspGI CCWGG 4 cut(s) 72, 253, 905, 955
PspN4I GGNNCC 4 cut(s) 18, 502, 729, 1185
PspPI GGNCC 3 cut(s) 16, 70, 1034
PspPPI RGGWCCY 2 cut(s) 16, 70
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 2 cut(s) 155, 260
PsuI RGATCY 1 cut(s) 1373
PvuII CAGCTG 1 cut(s) 1361
RsaI GTAC 3 cut(s) 340, 502, 739
RsaNI GTAC 3 cut(s) 339, 501, 738
SacI GAGCTC 1 cut(s) 1397
SapI GCTCTTC 2 cut(s) 38, 1143
SaqAI TTAA 3 cut(s) 638, 1196, 1287
SatI GCNGC 5 cut(s) 400, 597, 927, 1221, 1362
Sau3AI GATC 3 cut(s) 24, 1207, 1373
Sau96I GGNCC 3 cut(s) 16, 70, 1034
ScaI AGTACT 1 cut(s) 340
SchI GAGTC 1 cut(s) 663
ScrFI CCNGG 6 cut(s) 74, 255, 897, 907, 957, 1008
SduI GDGCHC 3 cut(s) 304, 1008, 1397
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 4 cut(s) 136, 598, 664, 833
SfcI CTRYAG 2 cut(s) 285, 714
SinI GGWCC 3 cut(s) 16, 70, 1034
SmlI CTYRAG 1 cut(s) 1105
SmoI CTYRAG 1 cut(s) 1105
Sse9I AATT 6 cut(s) 113, 419, 436, 882, 1081, 1134
SsiI CCGC 9 cut(s) 21, 57, 397, 498, 941, 1044, 1069, 1275, 1341
SspMI CTAG 2 cut(s) 795, 1371
SstI GAGCTC 1 cut(s) 1397
StyD4I CCNGG 6 cut(s) 72, 253, 895, 905, 955, 1006
StyI CCWWGG 2 cut(s) 917, 1025
TaaI ACNGT 2 cut(s) 998, 1324
TaiI ACGT 4 cut(s) 447, 535, 1023, 1122
TaqI TCGA 4 cut(s) 6, 66, 182, 742
TasI AATT 6 cut(s) 113, 419, 436, 882, 1081, 1134
TatI WGTACW 1 cut(s) 338
TfiI GAWTC 6 cut(s) 164, 448, 689, 1251, 1262, 1331
Tru1I TTAA 3 cut(s) 638, 1196, 1287
Tru9I TTAA 3 cut(s) 638, 1196, 1287
TscAI CASTG 2 cut(s) 826, 1276
TseFI GTSAC 2 cut(s) 171, 1307
TseI GCWGC 5 cut(s) 399, 596, 926, 1220, 1361
Tsp45I GTSAC 2 cut(s) 171, 1307
TspDTI ATGAA 2 cut(s) 306, 1376
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 2 cut(s) 826, 1276
VneI GTGCAC 1 cut(s) 300
VpaK11BI GGWCC 3 cut(s) 16, 70, 1034
XapI RAATTY 4 cut(s) 419, 882, 1081, 1134
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 1 cut(s) 854
XmnI GAANNNNTTC 1 cut(s) 1119
XspI CTAG 2 cut(s) 795, 1371
ZraI GACGTC 1 cut(s) 533
ZrmI AGTACT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.