Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012952

Physical Location & Seq

Genomic Coordinates Reverse (-)

30226999 .. 30231421

Gene Structure 4423 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1220100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2364 bp

ATGCCTGTGTCGACTCGGTCTCAGACCAACTCAGTGGAGAGAAGCAAACCCGGTTCAGATCAAGAACCGAGCCACGCGAATCTTCCGCTAAGGAACCCACATCATGGATTGAAGGAAAAGATGAGAGCTTTGACGCTGCTCTACGAGCAGCAGAAGCAGGCCACGGCAGCGCTGAGGAACCCGAATCCGAAATCCGATCCGGAGCAGGTCCGATTCGCGAGCCACCCGAGCGTCGAGCTTTTCGGTTCTTGCAAAAAAGAGGAGAGAGAAGAACCGAAGCAGGGGAGCCACCACGTGATGAGAGAGAACAACAGCAACGCAATGCACAACTCCACCGTTACCAGAACTTACGTTCTGCCACAGCCGCCGCCAACCCACGCCAAGGAGAACGTGGCAGCGGCTGGATTAAACGACCGGATTCTCAGCTTTACTTCGTGTCCAAGAAAGAGCAAGGCTTCAACCCCGGTGGCGAGGAGGCTGTCAATGGGAGGTCACGTGCAGCACGTGCCGGGGCCGCCGCGGGAGTCCAGTGGGGTGGCGGAGGGGGCTAGGAATGTGCAAGAATCGGAGACCCCTTCGGTTTCGGAGACACCCACGAGTCGGATTCTTGTGTTTGTTAGGCTGAGGCCGATGGCCAAGAAGGAAAAGGAGGCGGGTTCCAGATGCTGCGTTCGAATTGTGAACCGGCGGGATGTTTACCTGACGGAGTTTGCGAACGAGAACGATTATCTGCGGCTGAAGAGGCTGCGGGGACGGCATTTCATGTTCGATGCTTCTTTCCCTGATTCCACTTCTCAGCAAGAAGTTTATTCCAGCACTACAGCAGAGCTTGTGGAGGCAGTACTGCAGGGGAGGAATGGATCAGTGTTCTGCTATGGTGCCACTGGAGCTGGAAAAACATACACAATGCTCGGCACGCTGGAGAATCCGGGAGTGATGGTTTTGGCAATCAAGGACCTCTTCACCAAGATAAGGCAGAGGAGCTGTGATGGAAACCATGTGGTTCATCTGTCCTATCTTGAGGTCTATAACGAAACCGTGAGGGATCTGCTCTCCCCCGGAAGGCCTCTTGTACTTCGAGAAGACAAACAGGGAATAGTGGCAGCCGGTCTTACACAATACAGAGCTTATTCCGCCGATGAAGTTATGGCATTACTTCAGCAAGGAAACCAAAACCGAACTACTGAACCAACTCGCGCTAATGAAACTTCCTCACGGTCCCATGCTATTTTGCAGGTTGTGATTGAATACCGAGTAAAAGATGCTTCTATGAATGTGGTCAACCGAGTAGGGAAGCTCTCACTAATTGATCTTGCCGGGTCAGAGAGAGCCCTTGCCACGGACCAGAGAACAGCTAGATCTCTTGAGGGTGCCAATATAAATCGGTCACTCTTAGCACTGAGCAGCTGCATCAATGCTCTTGTGGAAGGAAAGAAGCACATACCTTATAGGAACTCAAAGTTAACCCAACTTCTTAAGGATTCTTTAGGAGGAGCTTGTAACACTGTGATGATCGCGAACATCAGCCCTTGCAATCTCACCTTTGGTGAAACCCAAAACACGCTTCATTGGGCAGATCGAGCAAAGGAAATCAGGACTAAGGCGTGCGAGGTGAATGAGGAGACACTGCAATTACCGGAAACTGATACTGATCAAGCCAAGCTATTGCTTGAATTACAGAAGGAGAATCGTGAACTGCGAGTACAACTTGCACACCAGAAACAGAAGCTATTAGTTGTCCAAGCACAGTCCTTCGCTGCAAATGCCTCTCCAACGCCTTCTTCGGTTGCATCCCTCTTGACTCCTCCAACTTCTGCCCAAGCAAATGACAAGCGGAAACCCAGACCCTCTTTCTTGACCCGGACTTGCTTCACACCAGAGTCCAAGAAAAGAGGGGCTGAGGAAGCAGTTAGAGATCTTCGCCGGACTGTAAAGACATTGGAGGCAGAAATAGAGAGGATGAAGAAAGATCACACTTCACAGCTAAAGCAGAAAGATGATCTCATTCGTGAGCTTTTGAGGGGAAGTGAGAAAACACCAGGAGCAGGGAGGGAGGGTGGGGAAAAGAGGGCAGTGACAAGGACTAGCTTGAGACTGCAGGAGCCGAGCACAAAGAGGATCGTTACAAGAGCTAGCATGAGAAAACAGGAGGCAAGCATTGGTGAATTGAAGAGCCCAAGTCACCGTTTCAGGTCACCAGTCCCAACAGCTAAAAAACGAAGCTTTTGGGACATAACAACTGCAAACAGCCCATCAGTTGCTACATTAAATGGAAGAAAAACTAGAAGCCATGTCATTTCTGAACCTGCTGCAGCTCCCTCCATGCTTCTTCAGGTTTTGCACGACAAAAGCCCGAGCGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0005929 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007019 GO:0008150 GO:0008574 GO:0009987 GO:0010938 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022411 GO:0030030 GO:0031109 GO:0031122 GO:0032984 GO:0032991 GO:0042623 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044782 GO:0051261 GO:0060404 GO:0061523 GO:0070462 GO:0071840 GO:0097435 GO:0120025 GO:0120036 GO:1903008 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

788

Amino Acids

87.28

Weight (kDa)

9.78

Isoelectric Point (pI)

52.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	202 - 351	1.4e-20	Microtubule binding
Kinesin	PF00225	207 - 531	2.8e-108	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012952)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18550
fragaria_vesca	FvH4_5g12880 FvH4_5g12880 FvH4_5g12880
malus_domestica	MD14G1220100.v1.1
prunus_persica	Prupe.5G214600_v2.0.a1 Prupe.5G214600_v2.0.a1 Prupe.5G214600_v2.0.a1
pyrus_communis	pycom14g18220
rosa_chinensis	RchiOBHm_Chr7g0184351
rosa_laevigata	RLG00000004983
rosa_multiflora	Rmu_sc0001557.1_g000023
rosa_roxburghii	Rroxscaffold_3G00269700
rosa_samantha	Rh7AG080800 Rh7BG066400 Rh7CG066700 Rh7DG066400
rosa_wichuraiana	Rw7G005450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	196, 1225, 2314
AccB1I	GGYRCC	2 cut(s)	878, 1370
AccB7I	CCANNNNNTGG	1 cut(s)	104
AccI	GTMKAC	1 cut(s)	11
AccII	CGCG	5 cut(s)	77, 218, 520, 1197, 1517
AccIII	TCCGGA	1 cut(s)	199
AclWI	GGATC	4 cut(s)	191, 868, 1053, 2126
AcoI	YGGCCR	1 cut(s)	633
AcuI	CTGAAG	3 cut(s)	758, 1142, 2315
AcvI	CACGTG	3 cut(s)	295, 496, 505
AdeI	CACNNNGTG	1 cut(s)	295
AfaI	GTAC	3 cut(s)	843, 1074, 1704
AfeI	AGCGCT	1 cut(s)	171
AfiI	CCNNNNNNNGG	8 cut(s)	104, 281, 382, 600, 972, 1062, 1339, 2045
AflII	CTTAAG	1 cut(s)	1475
AgsI	TTSAA	5 cut(s)	112, 459, 1247, 1673, 2170
AjnI	CCWGG	1 cut(s)	2038
AjuI	GAANNNNNNNTTGG	4 cut(s)	1548, 1580, 1765, 1797
Alw21I	GWGCWC	1 cut(s)	2111
Alw26I	GTCTC	5 cut(s)	24, 563, 581, 1616, 2086
AlwI	GGATC	4 cut(s)	191, 868, 1053, 2126
AlwNI	CAGNNNCTG	2 cut(s)	401, 666
Ama87I	CYCGRG	2 cut(s)	226, 2353
Aor13HI	TCCGGA	1 cut(s)	199
Aor51HI	AGCGCT	1 cut(s)	171
AoxI	GGCC	5 cut(s)	159, 512, 626, 633, 1064
AspLEI	GCGC	2 cut(s)	172, 1199
AspS9I	GGNCC	5 cut(s)	208, 512, 955, 1218, 1342
AsuC2I	CCSGG	7 cut(s)	51, 464, 510, 930, 1059, 1318, 1861
AsuHPI	GGTGA	7 cut(s)	955, 1531, 1559, 1624, 2174, 2174, 2187
AsuII	TTCGAA	1 cut(s)	673
AsuNHI	GCTAGC	1 cut(s)	2132
AvaI	CYCGRG	2 cut(s)	226, 2353
AvaII	GGWCC	4 cut(s)	208, 955, 1218, 1342
BalI	TGGCCA	1 cut(s)	635
BanI	GGYRCC	2 cut(s)	878, 1370
BanII	GRGCYC	2 cut(s)	1333, 2177
BauI	CACGAG	1 cut(s)	595
BbrPI	CACGTG	3 cut(s)	295, 496, 505
BbsI	GAAGAC	1 cut(s)	1089
Bbv12I	GWGCWC	1 cut(s)	2111
BbvCI	CCTCAGC	3 cut(s)	173, 623, 1899
BccI	CCATC	4 cut(s)	625, 931, 983, 2260
BceAI	ACGGC	2 cut(s)	180, 770
BciT130I	CCWGG	1 cut(s)	2040
BclI	TGATCA	1 cut(s)	1651
BcnI	CCSGG	7 cut(s)	51, 464, 510, 930, 1059, 1318, 1861
BcoDI	GTCTC	5 cut(s)	24, 563, 581, 1616, 2086
BfaI	CTAG	5 cut(s)	549, 1356, 2085, 2133, 2283
BfmI	CTRYAG	4 cut(s)	819, 845, 2096, 2310
BfoI	RGCGCY	1 cut(s)	173
BfrI	CTTAAG	1 cut(s)	1475
BfuAI	ACCTGC	3 cut(s)	196, 1225, 2314
BglII	AGATCT	2 cut(s)	1358, 1915
BmcAI	AGTACT	1 cut(s)	843
Bme1390I	CCNGG	8 cut(s)	51, 464, 510, 930, 1059, 1318, 1861, 2040
Bme18I	GGWCC	4 cut(s)	208, 955, 1218, 1342
BmeT110I	CYCGRG	2 cut(s)	226, 2353
BmgT120I	GGNCC	5 cut(s)	208, 512, 955, 1218, 1342
BmiI	GGNNCC	9 cut(s)	95, 179, 287, 513, 658, 880, 1220, 1372, 2103
BmrFI	CCNGG	8 cut(s)	51, 464, 510, 930, 1059, 1318, 1861, 2040
BmsI	GCATC	5 cut(s)	653, 760, 1252, 1419, 1799
BmtI	GCTAGC	1 cut(s)	2136
BpiI	GAAGAC	1 cut(s)	1089
BpmI	CTGGAG	2 cut(s)	906, 941
Bpu10I	CCTNAGC	4 cut(s)	89, 173, 623, 1899
Bpu14I	TTCGAA	1 cut(s)	673
BpuEI	CTTGAG	3 cut(s)	1040, 1385, 2110
BpuMI	CCSGG	7 cut(s)	51, 464, 510, 930, 1059, 1318, 1861
BsaAI	YACGTR	3 cut(s)	295, 496, 505
BsaBI	GATNNNNATC	1 cut(s)	1650
BsaI	GGTCTC	2 cut(s)	24, 563
BsaJI	CCNNGG	7 cut(s)	162, 381, 462, 509, 518, 1057, 1338
BsaWI	WCCGGW	3 cut(s)	199, 414, 1636
BsaXI	ACNNNNNCTCC	6 cut(s)	480, 510, 924, 954, 2041, 2071
Bsc4I	CCNNNNNNNGG	8 cut(s)	104, 281, 382, 600, 972, 1062, 1339, 2045
Bse118I	RCCGGY	2 cut(s)	684, 1106
Bse1I	ACTGG	3 cut(s)	528, 889, 2198
Bse3DI	GCAATG	1 cut(s)	327
Bse8I	GATNNNNATC	1 cut(s)	1650
BseAI	TCCGGA	1 cut(s)	199
BseBI	CCWGG	1 cut(s)	2040
BseDI	CCNNGG	7 cut(s)	162, 381, 462, 509, 518, 1057, 1338
BseGI	GGATG	3 cut(s)	697, 1790, 1965
BseJI	GATNNNNATC	1 cut(s)	1650
BseLI	CCNNNNNNNGG	8 cut(s)	104, 281, 382, 600, 972, 1062, 1339, 2045
BseMI	GCAATG	1 cut(s)	327
BseMII	CTCAG	8 cut(s)	35, 45, 164, 436, 614, 809, 1391, 1890
BseNI	ACTGG	3 cut(s)	528, 889, 2198
BseRI	GAGGAG	6 cut(s)	275, 487, 994, 1506, 1634, 1794
BsgI	GTGCAG	1 cut(s)	518
Bsh1236I	CGCG	5 cut(s)	77, 218, 520, 1197, 1517
Bsh1285I	CGRYCG	1 cut(s)	415
BshFI	GGCC	5 cut(s)	161, 514, 628, 635, 1066
BshNI	GGYRCC	2 cut(s)	878, 1370
BsiEI	CGRYCG	1 cut(s)	415
BsiHKAI	GWGCWC	1 cut(s)	2111
BsiHKCI	CYCGRG	2 cut(s)	226, 2353
BslFI	GGGAC	4 cut(s)	765, 1204, 2186, 2243
BslI	CCNNNNNNNGG	8 cut(s)	104, 281, 382, 600, 972, 1062, 1339, 2045
BsmAI	GTCTC	5 cut(s)	24, 563, 581, 1616, 2086
BsmFI	GGGAC	4 cut(s)	765, 1204, 2186, 2243
BsnI	GGCC	5 cut(s)	161, 514, 628, 635, 1066
Bso31I	GGTCTC	2 cut(s)	24, 563
BsoBI	CYCGRG	2 cut(s)	226, 2353
Bsp119I	TTCGAA	1 cut(s)	673
Bsp1286I	GDGCHC	3 cut(s)	1333, 2111, 2177
Bsp13I	TCCGGA	1 cut(s)	199
Bsp68I	TCGCGA	2 cut(s)	218, 1517
BspANI	GGCC	5 cut(s)	161, 514, 628, 635, 1066
BspCNI	CTCAG	8 cut(s)	34, 44, 165, 435, 615, 808, 1392, 1891
BspEI	TCCGGA	1 cut(s)	199
BspFNI	CGCG	5 cut(s)	77, 218, 520, 1197, 1517
BspLI	GGNNCC	9 cut(s)	95, 179, 287, 513, 658, 880, 1220, 1372, 2103
BspMAI	CTGCAG	3 cut(s)	849, 2100, 2314
BspMI	ACCTGC	3 cut(s)	196, 1225, 2314
BspOI	GCTAGC	1 cut(s)	2136
BspPI	GGATC	4 cut(s)	191, 868, 1053, 2126
BspQI	GCTCTTC	1 cut(s)	2165
BspT104I	TTCGAA	1 cut(s)	673
BspT107I	GGYRCC	2 cut(s)	878, 1370
BspTI	CTTAAG	1 cut(s)	1475
BspTNI	GGTCTC	2 cut(s)	24, 563
BsrDI	GCAATG	1 cut(s)	327
BsrFI	RCCGGY	2 cut(s)	684, 1106
BsrI	ACTGG	3 cut(s)	528, 889, 2198
BssAI	RCCGGY	2 cut(s)	684, 1106
BssECI	CCNNGG	7 cut(s)	162, 381, 462, 509, 518, 1057, 1338
BssSI	CACGAG	1 cut(s)	595
BssT1I	CCWWGG	1 cut(s)	381
Bst2BI	CACGAG	1 cut(s)	595
Bst2UI	CCWGG	1 cut(s)	2040
Bst4CI	ACNGT	7 cut(s)	337, 1039, 1218, 1507, 1749, 1930, 2186
Bst6I	CTCTTC	3 cut(s)	734, 965, 2165
BstAFI	CTTAAG	1 cut(s)	1475
BstAPI	GCANNNNNTGC	1 cut(s)	505
BstBAI	YACGTR	3 cut(s)	295, 496, 505
BstBI	TTCGAA	1 cut(s)	673
BstC8I	GCNNGC	6 cut(s)	159, 220, 917, 1606, 2134, 2155
BstDSI	CCRYGG	3 cut(s)	162, 518, 1338
BstEII	GGTNACC	1 cut(s)	2193
BstF5I	GGATG	3 cut(s)	697, 1790, 1965
BstFNI	CGCG	5 cut(s)	77, 218, 520, 1197, 1517
BstH2I	RGCGCY	1 cut(s)	173
BstHHI	GCGC	2 cut(s)	172, 1199
BstMAI	GTCTC	5 cut(s)	24, 563, 581, 1616, 2086
BstMCI	CGRYCG	1 cut(s)	415
BstNI	CCWGG	1 cut(s)	2040
BstPI	GGTNACC	1 cut(s)	2193
BstSCI	CCNGG	8 cut(s)	49, 462, 508, 928, 1057, 1316, 1859, 2038
BstSFI	CTRYAG	4 cut(s)	819, 845, 2096, 2310
BstUI	CGCG	5 cut(s)	77, 218, 520, 1197, 1517
BstV2I	GAAGAC	1 cut(s)	1089
BstX2I	RGATCY	3 cut(s)	1045, 1358, 1915
BstXI	CCANNNNNNTGG	2 cut(s)	34, 535
BstYI	RGATCY	3 cut(s)	1045, 1358, 1915
BsuRI	GGCC	5 cut(s)	161, 514, 628, 635, 1066
BtgI	CCRYGG	3 cut(s)	162, 518, 1338
BtsCI	GGATG	3 cut(s)	697, 1790, 1965
BtsI	GCAGTG	2 cut(s)	1625, 2079
BtsIMutI	CAGTG	8 cut(s)	39, 535, 870, 882, 1397, 1503, 1625, 2079
BtuMI	TCGCGA	2 cut(s)	218, 1517
BveI	ACCTGC	3 cut(s)	196, 1225, 2314
Cac8I	GCNNGC	6 cut(s)	159, 220, 917, 1606, 2134, 2155
CaiI	CAGNNNCTG	2 cut(s)	401, 666
CfoI	GCGC	2 cut(s)	172, 1199
Cfr10I	RCCGGY	2 cut(s)	684, 1106
Cfr13I	GGNCC	5 cut(s)	208, 512, 955, 1218, 1342
Cfr42I	CCGCGG	1 cut(s)	521
CseI	GACGC	2 cut(s)	142, 220
Csp6I	GTAC	3 cut(s)	842, 1073, 1703
CspCI	CAANNNNNGTGG	2 cut(s)	447, 482
CviAII	CATG	7 cut(s)	104, 763, 998, 1223, 2137, 2291, 2323
CviQI	GTAC	3 cut(s)	842, 1073, 1703
DraIII	CACNNNGTG	1 cut(s)	295
EaeI	YGGCCR	1 cut(s)	633
Eam1104I	CTCTTC	3 cut(s)	734, 965, 2165
EarI	CTCTTC	3 cut(s)	734, 965, 2165
EciI	GGCGGA	2 cut(s)	554, 1123
Eco130I	CCWWGG	1 cut(s)	381
Eco147I	AGGCCT	1 cut(s)	1066
Eco24I	GRGCYC	2 cut(s)	1333, 2177
Eco31I	GGTCTC	2 cut(s)	24, 563
Eco47I	GGWCC	4 cut(s)	208, 955, 1218, 1342
Eco47III	AGCGCT	1 cut(s)	171
Eco57I	CTGAAG	3 cut(s)	758, 1142, 2315
Eco72I	CACGTG	3 cut(s)	295, 496, 505
Eco88I	CYCGRG	2 cut(s)	226, 2353
Eco91I	GGTNACC	1 cut(s)	2193
EcoO109I	RGGNCCY	1 cut(s)	955
EcoO65I	GGTNACC	1 cut(s)	2193
EcoRII	CCWGG	1 cut(s)	2038
EcoT14I	CCWWGG	1 cut(s)	381
EcoT38I	GRGCYC	2 cut(s)	1333, 2177
ErhI	CCWWGG	1 cut(s)	381
FaeI	CATG	7 cut(s)	107, 766, 1001, 1226, 2140, 2294, 2326
FalI	AAGNNNNNCTT	2 cut(s)	944, 976
FaqI	GGGAC	4 cut(s)	765, 1204, 2186, 2243
FatI	CATG	7 cut(s)	103, 762, 997, 1222, 2136, 2290, 2322
FauI	CCCGC	4 cut(s)	513, 646, 681, 741
FbaI	TGATCA	1 cut(s)	1651
FblI	GTMKAC	1 cut(s)	11
FokI	GGATG	3 cut(s)	704, 1777, 1972
FriOI	GRGCYC	2 cut(s)	1333, 2177
FspBI	CTAG	5 cut(s)	549, 1356, 2085, 2133, 2283
GlaI	GCGC	2 cut(s)	171, 1198
GsuI	CTGGAG	2 cut(s)	906, 941
HaeII	RGCGCY	1 cut(s)	173
HaeIII	GGCC	5 cut(s)	161, 514, 628, 635, 1066
HgaI	GACGC	2 cut(s)	142, 220
HhaI	GCGC	2 cut(s)	172, 1199
Hin1II	CATG	7 cut(s)	107, 766, 1001, 1226, 2140, 2294, 2326
Hin6I	GCGC	2 cut(s)	170, 1197
HinP1I	GCGC	2 cut(s)	170, 1197
HincII	GTYRAC	3 cut(s)	12, 1282, 1464
HindII	GTYRAC	3 cut(s)	12, 1282, 1464
HindIII	AAGCTT	1 cut(s)	2221
HpaI	GTTAAC	1 cut(s)	1464
HphI	GGTGA	7 cut(s)	955, 1531, 1559, 1624, 2174, 2174, 2187
Hpy166II	GTNNAC	6 cut(s)	12, 682, 697, 1282, 1464, 1694
Hpy8I	GTNNAC	6 cut(s)	12, 682, 697, 1282, 1464, 1694
Hpy99I	CGWCG	1 cut(s)	236
HpyAV	CCTTC	8 cut(s)	106, 585, 634, 1056, 1421, 1675, 1762, 1788
HpyCH4III	ACNGT	7 cut(s)	337, 1039, 1218, 1507, 1749, 1930, 2186
HpyCH4IV	ACGT	5 cut(s)	294, 351, 390, 495, 504
HpySE526I	ACGT	5 cut(s)	294, 351, 390, 495, 504
Hsp92II	CATG	7 cut(s)	107, 766, 1001, 1226, 2140, 2294, 2326
HspAI	GCGC	2 cut(s)	170, 1197
Kpn2I	TCCGGA	1 cut(s)	199
Ksp22I	TGATCA	1 cut(s)	1651
KspAI	GTTAAC	1 cut(s)	1464
KspI	CCGCGG	1 cut(s)	521
LguI	GCTCTTC	1 cut(s)	2165
LmnI	GCTCC	8 cut(s)	202, 285, 887, 981, 1493, 2042, 2101, 2320
LweI	GCATC	5 cut(s)	653, 760, 1252, 1419, 1799
MaeI	CTAG	5 cut(s)	549, 1356, 2085, 2133, 2283
MaeII	ACGT	5 cut(s)	294, 351, 390, 495, 504
MaeIII	GTNAC	8 cut(s)	337, 491, 1386, 1499, 2074, 2122, 2180, 2193
MflI	RGATCY	3 cut(s)	1045, 1358, 1915
MhlI	GDGCHC	3 cut(s)	1333, 2111, 2177
MlsI	TGGCCA	1 cut(s)	635
MluCI	AATT	5 cut(s)	675, 1305, 1631, 1673, 2165
MluNI	TGGCCA	1 cut(s)	635
MlyI	GAGTC	5 cut(s)	7, 533, 607, 1795, 1889
MmeI	TCCRAC	3 cut(s)	581, 1796, 1832
Mox20I	TGGCCA	1 cut(s)	635
MroI	TCCGGA	1 cut(s)	199
MscI	TGGCCA	1 cut(s)	635
MseI	TTAA	4 cut(s)	407, 1463, 1476, 2267
MslI	CAYNNNNRTG	1 cut(s)	1508
Msp20I	TGGCCA	1 cut(s)	635
MspA1I	CMGCKG	3 cut(s)	398, 520, 1407
MspCI	CTTAAG	1 cut(s)	1475
MspR9I	CCNGG	8 cut(s)	51, 464, 510, 930, 1059, 1318, 1861, 2040
MvaI	CCWGG	1 cut(s)	2040
MvnI	CGCG	5 cut(s)	77, 218, 520, 1197, 1517
NciI	CCSGG	7 cut(s)	51, 464, 510, 930, 1059, 1318, 1861
NheI	GCTAGC	1 cut(s)	2132
NlaIII	CATG	7 cut(s)	107, 766, 1001, 1226, 2140, 2294, 2326
NlaIV	GGNNCC	9 cut(s)	95, 179, 287, 513, 658, 880, 1220, 1372, 2103
NmeAIII	GCCGAG	2 cut(s)	891, 2130
NmuCI	GTSAC	5 cut(s)	491, 1386, 2074, 2180, 2193
NruI	TCGCGA	2 cut(s)	218, 1517
NspV	TTCGAA	1 cut(s)	673
PceI	AGGCCT	1 cut(s)	1066
PciSI	GCTCTTC	1 cut(s)	2165
PcsI	WCGNNNNNNNCGW	3 cut(s)	501, 710, 1696
PflFI	GACNNNGTC	1 cut(s)	16
PflMI	CCANNNNNTGG	1 cut(s)	104
PfoI	TCCNGGA	1 cut(s)	928
PleI	GAGTC	5 cut(s)	7, 532, 606, 1795, 1888
PmaCI	CACGTG	3 cut(s)	295, 496, 505
PmlI	CACGTG	3 cut(s)	295, 496, 505
PpsI	GAGTC	5 cut(s)	7, 532, 606, 1795, 1888
Ppu21I	YACGTR	3 cut(s)	295, 496, 505
PpuMI	RGGWCCY	1 cut(s)	955
Psp5II	RGGWCCY	1 cut(s)	955
Psp6I	CCWGG	1 cut(s)	2038
PspCI	CACGTG	3 cut(s)	295, 496, 505
PspEI	GGTNACC	1 cut(s)	2193
PspGI	CCWGG	1 cut(s)	2038
PspN4I	GGNNCC	9 cut(s)	95, 179, 287, 513, 658, 880, 1220, 1372, 2103
PspPI	GGNCC	5 cut(s)	208, 512, 955, 1218, 1342
PspPPI	RGGWCCY	1 cut(s)	955
PsrI	GAACNNNNNNTAC	2 cut(s)	1686, 1718
PstI	CTGCAG	3 cut(s)	849, 2100, 2314
PstNI	CAGNNNCTG	2 cut(s)	401, 666
PsuI	RGATCY	3 cut(s)	1045, 1358, 1915
PsyI	GACNNNGTC	1 cut(s)	16
PvuII	CAGCTG	1 cut(s)	1407
RruI	TCGCGA	2 cut(s)	218, 1517
RsaI	GTAC	3 cut(s)	843, 1074, 1704
RsaNI	GTAC	3 cut(s)	842, 1073, 1703
RseI	CAYNNNNRTG	1 cut(s)	1508
SacII	CCGCGG	1 cut(s)	521
SalI	GTCGAC	1 cut(s)	10
SapI	GCTCTTC	1 cut(s)	2165
SaqAI	TTAA	4 cut(s)	407, 1463, 1476, 2267
Sau96I	GGNCC	5 cut(s)	208, 512, 955, 1218, 1342
ScaI	AGTACT	1 cut(s)	843
SchI	GAGTC	5 cut(s)	7, 533, 607, 1795, 1889
ScrFI	CCNGG	8 cut(s)	51, 464, 510, 930, 1059, 1318, 1861, 2040
SduI	GDGCHC	3 cut(s)	1333, 2111, 2177
SfaNI	GCATC	5 cut(s)	653, 760, 1252, 1419, 1799
SfcI	CTRYAG	4 cut(s)	819, 845, 2096, 2310
Sfr303I	CCGCGG	1 cut(s)	521
SfuI	TTCGAA	1 cut(s)	673
SgrBI	CCGCGG	1 cut(s)	521
SinI	GGWCC	4 cut(s)	208, 955, 1218, 1342
SmiMI	CAYNNNNRTG	1 cut(s)	1508
SmlI	CTYRAG	4 cut(s)	1019, 1364, 1475, 2089
SmoI	CTYRAG	4 cut(s)	1019, 1364, 1475, 2089
Sse9I	AATT	5 cut(s)	675, 1305, 1631, 1673, 2165
SseBI	AGGCCT	1 cut(s)	1066
SspMI	CTAG	5 cut(s)	549, 1356, 2085, 2133, 2283
StuI	AGGCCT	1 cut(s)	1066
StyD4I	CCNGG	8 cut(s)	49, 462, 508, 928, 1057, 1316, 1859, 2038
StyI	CCWWGG	1 cut(s)	381
TaaI	ACNGT	7 cut(s)	337, 1039, 1218, 1507, 1749, 1930, 2186
TaiI	ACGT	5 cut(s)	297, 354, 393, 498, 507
TaqI	TCGA	6 cut(s)	11, 234, 673, 768, 1078, 1579
TaqII	GACCGA	2 cut(s)	6, 1374
TasI	AATT	5 cut(s)	675, 1305, 1631, 1673, 2165
TatI	WGTACW	3 cut(s)	841, 1072, 1702
TauI	GCSGC	6 cut(s)	367, 370, 401, 517, 520, 736
Tru1I	TTAA	4 cut(s)	407, 1463, 1476, 2267
Tru9I	TTAA	4 cut(s)	407, 1463, 1476, 2267
TscAI	CASTG	8 cut(s)	39, 535, 870, 889, 1404, 1510, 1632, 2079
TseFI	GTSAC	5 cut(s)	491, 1386, 2074, 2180, 2193
Tsp45I	GTSAC	5 cut(s)	491, 1386, 2074, 2180, 2193
TspDTI	ATGAA	7 cut(s)	751, 995, 1155, 1218, 1286, 1556, 1976
TspGWI	ACGGA	2 cut(s)	719, 1355
TspRI	CASTG	8 cut(s)	39, 535, 870, 889, 1404, 1510, 1632, 2079
Tth111I	GACNNNGTC	1 cut(s)	16
Van91I	CCANNNNNTGG	1 cut(s)	104
Vha464I	CTTAAG	1 cut(s)	1475
VpaK11BI	GGWCC	4 cut(s)	208, 955, 1218, 1342
XcmI	CCANNNNNNNNNTGG	1 cut(s)	388
XmiI	GTMKAC	1 cut(s)	11
XspI	CTAG	5 cut(s)	549, 1356, 2085, 2133, 2283
ZrmI	AGTACT	1 cut(s)	843

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.