Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0012952

Physical Location & Seq

Genomic Coordinates Forward (+)

64158595 .. 64162697

Gene Structure 4103 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004983

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGCCTGTATCAACTCGTTCGCAAAGTGAACGGAAACCCAGGTCGGATCAAGAACAGACCCACTCGAATCTTCTTCCTCTGCGAAACCCACACCATGGATTGAAGGAAAAGATGAAAGCTTTGACTCTACTCTACGAGCAGCAGAAGCAAGCCTCCGCAGCTCTGAGAAGCTCGAAGCCCCCGGACCCGTCGGAGCTCCGACTCGCGACCCAGAAGTGCAGAAAAGAAGAGCACGAGTCGGGGCTCCGACCCGGAAGCCGGGTCGTGATGAGAGAGAACAACGCGATGCACAACTCCGCCGTTGCAAGAACCTACGTTCTGCCTCTGCCTCCGGCCCAGTTTCAGGCCCAGGCCCAACTACAAGCCCACGACGACGCGAAGGAGAATGCGGCGGTCGCCGGAACCACCACCACCGACCGGATTCTCAGCTTCACTTCGCTTCCAAGAAAGGCCAGGCACTCGACGACCACGGTGGCGCGGCGGCTCTCCCTCGGCGGAGGAGGAGGTGGAGGTCAGATTTCTCACGTGGCGCCGGAGATGGAGAATGTGCGAGAATTGGAGACGAAATTTGGGGCTTCGGAGACGAAAGTCCCAAATTGTGGGAGCCGGATTATGGTGTTTGTGAGGCTTCGGCCGATGAGCAAGAAGGAGAAGGAGGGGGGTTCTCGGTGTTGTGTGAGGATCGTGAACCGGCGTGACGTGTACTTGACGGAGTTTGCGAACGAGAACGATTATCTGAGGCTGAAGAGGCTGAGGGGACGGCATTTCACGTTTGATGCTTCGTTTCCAGATTCTACTTCTCAGCAGGAAGTTTATTCCACCAGTACAGCTGAGCTTGTGGAAGCAGTTCTGCAGGGGAGGAATGGATCAGTTTTCTGCTATGGTGCCACGGGAGCTGGGAAAACGTACACAATGCTGGGCACTCTGGAGAGTCCAGGAGTGATGGTTTTGGCGTTCAAGGACCTCTTTACAAAGATAAGGCAGAGGAGCTGTGATGGAAATCATGTGGTTCATCTGTCCTATCTTGAAGTCTATAATGAAACTGTCAGGGATTTGCTCTCCCCCGGACGGCCTCTGGTTCTTAGAGAAGATAAACAGGGAATGGTGGCAGCCGGTCTTACACAATACAGAGCTTATTCCACCGATGAAGTTATGCAATTGCTTCAGCGGGGAAACCAAAACCGAACTACTGAACCAACTCGCTGTAATGAAACTTCTTCTCGCTCCCATGCTATTTTACAGGTTGTGGTTGAATACCAGGTAAAAGATGCTTCTATGAATGTGGTCAACAGAGTAGGCAAGCTTTCACTTATTGATCTTGCCGGGTCCGAGAGAGCCCTTGCCACGGATCAGAGAACAGTAAGATCTATTGAGGGTGCCAATATAAATCGGTCACTCCTAGCACTGAGCAGCTGCATCAATGCTCTCGTGGAGGGAAAGAAGCACATACCTTATCGAAATTCAAAGTTGACCCAACTTCTCAAGGATTCTCTAGGAGGAGATTGTAACACTGTAATGATTGCCAACATCAGCCCATGTAACCTCTCATTTGGTGAAACCCAAAACACCCTTCACTGGGCTGATCGAGCAAAGGAAATTAGGACCAAGGCCTCTGAGGTGAACGAGGAAGTAGTGGAAGTACCTGAATCTGAAACTGATCGTGCCAAACTATTACTTGAACTACAGAAAGAGAACCGTGAACTTCGAGTACAACTGGCACGTCATCAACAGAAACTATTAGTCGTCCAAGCACAATCCTTGGCTGCCAATGCATCTCCAACCCCTTCTTCAGCTACCTCTCTCTTGACTCCTCCGTCGTCTGCCCAAGCAAATGAGAAGAGAAAACCCAGACCATCTTTCTTGGCTAGGAATTGTTTCACACCTGAATCCAAAAGAAGAGGGGCTGATGAAGGAGTTAGAGAGCTTCAAAAGACAGTAAAAGCATTAGAAGCAGAAATAGAAAGGATGAAGAAGGATCATACTTCACAGCTAAAGCAGAAGGATAATCTTATTCGTGATCTTTCACGGAAAAGTGACCAACCACTAGCACCAGCAGTGGGGAGGGAGGGGACAAAGAGGGTAGTCACAAGGGCTAGCTTGAGACCAAAGGAGCCAAGCACAGGTGAGTTGAAGAGTCCAAGCCATCGTTTCAGGTCACCAGTCCCAACAGCGAAAAAACGAAGCTTTTGGGACATAACAACCGCAAATAGCCCATCAGTTACTACACACAATGGAAGGAAAACTAGAAGTCATGTTATTTGTGAACCACCTCCTGTTCCCTCCATGCTTCTCCAGCCAGGTTTTGCACGACAAAAGCCCGAGCGTCTGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0005929 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007019 GO:0008150 GO:0008574 GO:0009987 GO:0010938 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022411 GO:0030030 GO:0031109 GO:0031122 GO:0032984 GO:0032991 GO:0042623 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044782 GO:0051261 GO:0060404 GO:0061523 GO:0070462 GO:0071840 GO:0097435 GO:0120025 GO:0120036 GO:1903008 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

778

Amino Acids

86.42

Weight (kDa)

9.83

Isoelectric Point (pI)

52.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	203 - 353	9e-21	Microtubule binding
Kinesin	PF00225	209 - 533	2.2e-108	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012952)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18550
fragaria_vesca	FvH4_5g12880 FvH4_5g12880 FvH4_5g12880
malus_domestica	MD14G1220100.v1.1
prunus_persica	Prupe.5G214600_v2.0.a1 Prupe.5G214600_v2.0.a1 Prupe.5G214600_v2.0.a1
pyrus_communis	pycom14g18220
rosa_chinensis	RchiOBHm_Chr7g0184351
rosa_laevigata	RLG00000004983
rosa_multiflora	Rmu_sc0001557.1_g000023
rosa_roxburghii	Rroxscaffold_3G00269700
rosa_samantha	Rh7AG080800 Rh7BG066400 Rh7CG066700 Rh7DG066400
rosa_wichuraiana	Rw7G005450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1813
AccB1I	GGYRCC	3 cut(s)	529, 884, 1376
AccB7I	CCANNNNNTGG	2 cut(s)	95, 599
AccII	CGCG	4 cut(s)	206, 284, 377, 478
AciI	CCGC	9 cut(s)	156, 297, 389, 392, 478, 481, 495, 1168, 2202
AclWI	GGATC	5 cut(s)	54, 689, 874, 1356, 1983
AcoI	YGGCCR	1 cut(s)	632
AcsI	RAATTY	2 cut(s)	566, 1459
AcuI	CTGAAG	3 cut(s)	764, 1148, 1773
AcvI	CACGTG	1 cut(s)	526
AcyI	GRCGYC	1 cut(s)	530
AfaI	GTAC	5 cut(s)	704, 826, 908, 1641, 1710
AflIII	ACRYGT	1 cut(s)	699
AgsI	TTSAA	8 cut(s)	103, 958, 1028, 1253, 1464, 1679, 1928, 2131
AjiI	CACGTC	2 cut(s)	700, 1721
AjnI	CCWGG	6 cut(s)	38, 348, 452, 934, 1257, 2296
AjuI	GAANNNNNNNTTGG	4 cut(s)	1554, 1586, 1771, 1803
Alw21I	GWGCWC	2 cut(s)	198, 234
Alw26I	GTCTC	3 cut(s)	554, 575, 2095
AlwI	GGATC	5 cut(s)	54, 689, 874, 1356, 1983
Ama87I	CYCGRG	1 cut(s)	2318
AoxI	GGCC	7 cut(s)	333, 345, 351, 450, 632, 1070, 1608
ApeKI	GCWGC	6 cut(s)	139, 158, 1109, 1410, 1413, 1763
ApoI	RAATTY	2 cut(s)	566, 1459
Asp700I	GAANNNNTTC	2 cut(s)	846, 1874
AspLEI	GCGC	2 cut(s)	478, 532
AspS9I	GGNCC	7 cut(s)	184, 334, 346, 352, 961, 1326, 1602
AsuC2I	CCSGG	5 cut(s)	182, 252, 260, 1065, 1324
AsuHPI	GGTGA	4 cut(s)	1565, 1630, 2135, 2148
AsuNHI	GCTAGC	1 cut(s)	2093
AvaI	CYCGRG	1 cut(s)	2318
AvaII	GGWCC	4 cut(s)	184, 961, 1326, 1602
BaeGI	GKGCMC	1 cut(s)	923
BanI	GGYRCC	3 cut(s)	529, 884, 1376
BanII	GRGCYC	3 cut(s)	198, 246, 1339
BarI	GAAGNNNNNNTAC	2 cut(s)	1198, 1230
BauI	CACGAG	2 cut(s)	233, 1427
BbrPI	CACGTG	1 cut(s)	526
Bbv12I	GWGCWC	2 cut(s)	198, 234
BbvCI	CCTCAGC	1 cut(s)	752
BbvI	GCAGC	6 cut(s)	151, 170, 1121, 1400, 1422, 1750
BccI	CCATC	6 cut(s)	532, 937, 989, 1861, 2151, 2221
BceAI	ACGGC	3 cut(s)	284, 776, 1085
BciT130I	CCWGG	6 cut(s)	40, 350, 454, 936, 1259, 2298
BcnI	CCSGG	5 cut(s)	182, 252, 260, 1065, 1324
BcoDI	GTCTC	3 cut(s)	554, 575, 2095
BfaI	CTAG	6 cut(s)	1400, 1493, 1866, 2045, 2094, 2244
BfmI	CTRYAG	2 cut(s)	851, 1682
BfoI	RGCGCY	1 cut(s)	533
BglII	AGATCT	1 cut(s)	1364
BisI	GCNGC	9 cut(s)	140, 159, 390, 479, 482, 1110, 1411, 1414, 1764
BlpI	GCTNAGC	1 cut(s)	831
BlsI	GCNGC	9 cut(s)	141, 160, 391, 480, 483, 1111, 1412, 1415, 1765
Bme18I	GGWCC	4 cut(s)	184, 961, 1326, 1602
BmeT110I	CYCGRG	1 cut(s)	2318
BmgBI	CACGTC	2 cut(s)	700, 1721
BmgT120I	GGNCC	7 cut(s)	184, 334, 346, 352, 961, 1326, 1602
BmiI	GGNNCC	9 cut(s)	186, 245, 403, 531, 605, 886, 1327, 1378, 2112
BmrI	ACTGGG	2 cut(s)	331, 1585
BmsI	GCATC	5 cut(s)	276, 766, 1258, 1425, 1781
BmtI	GCTAGC	1 cut(s)	2097
BmuI	ACTGGG	2 cut(s)	331, 1585
BoxI	GACNNNNGTC	1 cut(s)	587
BpmI	CTGGAG	2 cut(s)	947, 2276
Bpu10I	CCTNAGC	1 cut(s)	752
Bpu1102I	GCTNAGC	1 cut(s)	831
BpuEI	CTTGAG	2 cut(s)	1466, 2119
BpuMI	CCSGG	5 cut(s)	182, 252, 260, 1065, 1324
BsaAI	YACGTR	1 cut(s)	526
BsaBI	GATNNNNATC	1 cut(s)	999
BsaHI	GRCGYC	1 cut(s)	530
BsaI	GGTCTC	1 cut(s)	2095
BsaWI	WCCGGW	1 cut(s)	417
BsaXI	ACNNNNNCTCC	2 cut(s)	930, 960
Bse118I	RCCGGY	2 cut(s)	690, 1112
Bse1I	ACTGG	5 cut(s)	337, 822, 1580, 1719, 2159
Bse8I	GATNNNNATC	1 cut(s)	999
BseBI	CCWGG	6 cut(s)	40, 350, 454, 936, 1259, 2298
BseGI	GGATG	1 cut(s)	1971
BseJI	GATNNNNATC	1 cut(s)	999
BseMII	CTCAG	8 cut(s)	155, 439, 728, 743, 815, 822, 1397, 1605
BseNI	ACTGG	5 cut(s)	337, 822, 1580, 1719, 2159
BseRI	GAGGAG	5 cut(s)	513, 516, 1000, 1512, 1800
BseSI	GKGCMC	1 cut(s)	923
BseX3I	CGGCCG	1 cut(s)	632
BseXI	GCAGC	6 cut(s)	151, 170, 1121, 1400, 1422, 1750
BseYI	CCCAGC	2 cut(s)	896, 916
BsgI	GTGCAG	1 cut(s)	238
Bsh1236I	CGCG	4 cut(s)	206, 284, 377, 478
Bsh1285I	CGRYCG	3 cut(s)	396, 418, 635
BshFI	GGCC	7 cut(s)	335, 347, 353, 452, 634, 1072, 1610
BshNI	GGYRCC	3 cut(s)	529, 884, 1376
BsiEI	CGRYCG	3 cut(s)	396, 418, 635
BsiHKAI	GWGCWC	2 cut(s)	198, 234
BsiHKCI	CYCGRG	1 cut(s)	2318
BslFI	GGGAC	5 cut(s)	575, 771, 2083, 2147, 2204
BsmAI	GTCTC	3 cut(s)	554, 575, 2095
BsmBI	CGTCTC	2 cut(s)	554, 575
BsmFI	GGGAC	5 cut(s)	575, 771, 2083, 2147, 2204
BsmI	GAATGC	1 cut(s)	391
BsnI	GGCC	7 cut(s)	335, 347, 353, 452, 634, 1072, 1610
Bso31I	GGTCTC	1 cut(s)	2095
BsoBI	CYCGRG	1 cut(s)	2318
Bsp1286I	GDGCHC	5 cut(s)	198, 234, 246, 923, 1339
Bsp1720I	GCTNAGC	1 cut(s)	831
Bsp19I	CCATGG	1 cut(s)	94
Bsp68I	TCGCGA	1 cut(s)	206
BspACI	CCGC	9 cut(s)	156, 297, 389, 392, 478, 481, 495, 1168, 2202
BspANI	GGCC	7 cut(s)	335, 347, 353, 452, 634, 1072, 1610
BspCNI	CTCAG	8 cut(s)	156, 438, 729, 744, 814, 823, 1398, 1606
BspFNI	CGCG	4 cut(s)	206, 284, 377, 478
BspLI	GGNNCC	9 cut(s)	186, 245, 403, 531, 605, 886, 1327, 1378, 2112
BspMAI	CTGCAG	1 cut(s)	855
BspOI	GCTAGC	1 cut(s)	2097
BspPI	GGATC	5 cut(s)	54, 689, 874, 1356, 1983
BspQI	GCTCTTC	1 cut(s)	222
BspT107I	GGYRCC	3 cut(s)	529, 884, 1376
BspTNI	GGTCTC	1 cut(s)	2095
BsrFI	RCCGGY	2 cut(s)	690, 1112
BsrI	ACTGG	5 cut(s)	337, 822, 1580, 1719, 2159
BssAI	RCCGGY	2 cut(s)	690, 1112
BssNI	GRCGYC	1 cut(s)	530
BssSI	CACGAG	2 cut(s)	233, 1427
BssT1I	CCWWGG	3 cut(s)	94, 1605, 1758
Bst2BI	CACGAG	2 cut(s)	233, 1427
Bst2UI	CCWGG	6 cut(s)	40, 350, 454, 936, 1259, 2298
Bst4CI	ACNGT	6 cut(s)	472, 1045, 1360, 1513, 1697, 1936
Bst6I	CTCTTC	5 cut(s)	222, 740, 1832, 1891, 2126
BstACI	GRCGYC	1 cut(s)	530
BstBAI	YACGTR	1 cut(s)	526
BstC8I	GCNNGC	3 cut(s)	150, 1301, 2095
BstDEI	CTNAG	9 cut(s)	164, 425, 737, 752, 801, 831, 1082, 1406, 1614
BstDSI	CCRYGG	4 cut(s)	94, 468, 888, 1344
BstEII	GGTNACC	1 cut(s)	2154
BstF5I	GGATG	1 cut(s)	1971
BstFNI	CGCG	4 cut(s)	206, 284, 377, 478
BstH2I	RGCGCY	1 cut(s)	533
BstHHI	GCGC	2 cut(s)	478, 532
BstMAI	GTCTC	3 cut(s)	554, 575, 2095
BstMCI	CGRYCG	3 cut(s)	396, 418, 635
BstMWI	GCNNNNNNNGC	9 cut(s)	145, 158, 395, 748, 893, 1586, 1769, 1949, 2293
BstNI	CCWGG	6 cut(s)	40, 350, 454, 936, 1259, 2298
BstPAI	GACNNNNGTC	1 cut(s)	587
BstPI	GGTNACC	1 cut(s)	2154
BstSFI	CTRYAG	2 cut(s)	851, 1682
BstSLI	GKGCMC	1 cut(s)	923
BstUI	CGCG	4 cut(s)	206, 284, 377, 478
BstV1I	GCAGC	6 cut(s)	151, 170, 1121, 1400, 1422, 1750
BstX2I	RGATCY	1 cut(s)	1364
BstYI	RGATCY	1 cut(s)	1364
BstZI	CGGCCG	1 cut(s)	632
BsuRI	GGCC	7 cut(s)	335, 347, 353, 452, 634, 1072, 1610
BtgI	CCRYGG	4 cut(s)	94, 468, 888, 1344
BtgZI	GCGATG	1 cut(s)	299
BtrI	CACGTC	2 cut(s)	700, 1721
BtsCI	GGATG	1 cut(s)	1971
BtsI	GCAGTG	1 cut(s)	2061
BtsIMutI	CAGTG	4 cut(s)	1403, 1509, 1573, 2061
BtuMI	TCGCGA	1 cut(s)	206
Cac8I	GCNNGC	3 cut(s)	150, 1301, 2095
CfoI	GCGC	2 cut(s)	478, 532
Cfr10I	RCCGGY	2 cut(s)	690, 1112
Cfr13I	GGNCC	7 cut(s)	184, 334, 346, 352, 961, 1326, 1602
CseI	GACGC	2 cut(s)	383, 2312
CsiI	ACCWGGT	1 cut(s)	1257
Csp6I	GTAC	5 cut(s)	703, 825, 907, 1640, 1709
CviAII	CATG	6 cut(s)	95, 1004, 1229, 1536, 2252, 2284
CviQI	GTAC	5 cut(s)	703, 825, 907, 1640, 1709
DdeI	CTNAG	9 cut(s)	164, 425, 737, 752, 801, 831, 1082, 1406, 1614
DinI	GGCGCC	1 cut(s)	531
DrdI	GACNNNNNNGTC	1 cut(s)	1813
DseDI	GACNNNNNNGTC	1 cut(s)	1813
EaeI	YGGCCR	1 cut(s)	632
EagI	CGGCCG	1 cut(s)	632
Eam1104I	CTCTTC	5 cut(s)	222, 740, 1832, 1891, 2126
EarI	CTCTTC	5 cut(s)	222, 740, 1832, 1891, 2126
EciI	GGCGGA	2 cut(s)	286, 510
Ecl136II	GAGCTC	1 cut(s)	196
EclXI	CGGCCG	1 cut(s)	632
Eco130I	CCWWGG	3 cut(s)	94, 1605, 1758
Eco147I	AGGCCT	1 cut(s)	1610
Eco24I	GRGCYC	3 cut(s)	198, 246, 1339
Eco31I	GGTCTC	1 cut(s)	2095
Eco47I	GGWCC	4 cut(s)	184, 961, 1326, 1602
Eco52I	CGGCCG	1 cut(s)	632
Eco53kI	GAGCTC	1 cut(s)	196
Eco57I	CTGAAG	3 cut(s)	764, 1148, 1773
Eco72I	CACGTG	1 cut(s)	526
Eco88I	CYCGRG	1 cut(s)	2318
Eco91I	GGTNACC	1 cut(s)	2154
EcoICRI	GAGCTC	1 cut(s)	196
EcoO109I	RGGNCCY	1 cut(s)	961
EcoO65I	GGTNACC	1 cut(s)	2154
EcoRII	CCWGG	6 cut(s)	38, 348, 452, 934, 1257, 2296
EcoT14I	CCWWGG	3 cut(s)	94, 1605, 1758
EcoT22I	ATGCAT	1 cut(s)	1774
EcoT38I	GRGCYC	3 cut(s)	198, 246, 1339
EgeI	GGCGCC	1 cut(s)	531
EheI	GGCGCC	1 cut(s)	531
ErhI	CCWWGG	3 cut(s)	94, 1605, 1758
Esp3I	CGTCTC	2 cut(s)	554, 575
FaeI	CATG	6 cut(s)	98, 1007, 1232, 1539, 2255, 2287
FalI	AAGNNNNNCTT	2 cut(s)	950, 982
FaqI	GGGAC	5 cut(s)	575, 771, 2083, 2147, 2204
FatI	CATG	6 cut(s)	94, 1003, 1228, 1535, 2251, 2283
FauI	CCCGC	1 cut(s)	1161
Fnu4HI	GCNGC	9 cut(s)	140, 159, 390, 479, 482, 1110, 1411, 1414, 1764
FokI	GGATG	1 cut(s)	1978
FriOI	GRGCYC	3 cut(s)	198, 246, 1339
Fsp4HI	GCNGC	9 cut(s)	140, 159, 390, 479, 482, 1110, 1411, 1414, 1764
FspBI	CTAG	6 cut(s)	1400, 1493, 1866, 2045, 2094, 2244
GlaI	GCGC	2 cut(s)	477, 531
GluI	GCNGC	9 cut(s)	140, 159, 390, 479, 482, 1110, 1411, 1414, 1764
GsaI	CCCAGC	2 cut(s)	900, 920
GsuI	CTGGAG	2 cut(s)	947, 2276
HaeII	RGCGCY	1 cut(s)	533
HaeIII	GGCC	7 cut(s)	335, 347, 353, 452, 634, 1072, 1610
HgaI	GACGC	2 cut(s)	383, 2312
HhaI	GCGC	2 cut(s)	478, 532
Hin1I	GRCGYC	1 cut(s)	530
Hin1II	CATG	6 cut(s)	98, 1007, 1232, 1539, 2255, 2287
Hin6I	GCGC	2 cut(s)	476, 530
HinP1I	GCGC	2 cut(s)	476, 530
HincII	GTYRAC	2 cut(s)	1288, 1470
HindII	GTYRAC	2 cut(s)	1288, 1470
HindIII	AAGCTT	3 cut(s)	117, 1301, 2182
HphI	GGTGA	4 cut(s)	1565, 1630, 2135, 2148
Hpy166II	GTNNAC	9 cut(s)	29, 688, 703, 909, 1288, 1470, 1621, 1700, 2264
Hpy188III	TCNNGA	9 cut(s)	50, 205, 265, 685, 788, 926, 1025, 1804, 2015
Hpy8I	GTNNAC	9 cut(s)	29, 688, 703, 909, 1288, 1470, 1621, 1700, 2264
Hpy99I	CGWCG	5 cut(s)	193, 374, 377, 466, 1819
HpyCH4III	ACNGT	6 cut(s)	472, 1045, 1360, 1513, 1697, 1936
HpyCH4IV	ACGT	6 cut(s)	315, 525, 699, 770, 905, 1720
HpyCH4V	TGCA	8 cut(s)	219, 289, 305, 853, 1156, 1416, 1772, 2306
HpyF10VI	GCNNNNNNNGC	9 cut(s)	145, 158, 395, 748, 893, 1586, 1769, 1949, 2293
HpyF3I	CTNAG	9 cut(s)	164, 425, 737, 752, 801, 831, 1082, 1406, 1614
HpySE526I	ACGT	6 cut(s)	315, 525, 699, 770, 905, 1720
Hsp92I	GRCGYC	1 cut(s)	530
Hsp92II	CATG	6 cut(s)	98, 1007, 1232, 1539, 2255, 2287
HspAI	GCGC	2 cut(s)	476, 530
KasI	GGCGCC	1 cut(s)	529
LguI	GCTCTTC	1 cut(s)	222
LmnI	GCTCC	8 cut(s)	193, 201, 249, 603, 893, 987, 1229, 2110
Lsp1109I	GCAGC	6 cut(s)	151, 170, 1121, 1400, 1422, 1750
LweI	GCATC	5 cut(s)	276, 766, 1258, 1425, 1781
MabI	ACCWGGT	1 cut(s)	1257
MaeI	CTAG	6 cut(s)	1400, 1493, 1866, 2045, 2094, 2244
MaeII	ACGT	6 cut(s)	315, 525, 699, 770, 905, 1720
MaeIII	GTNAC	8 cut(s)	695, 1392, 1505, 1538, 2033, 2083, 2154, 2218
MfeI	CAATTG	1 cut(s)	1157
MflI	RGATCY	1 cut(s)	1364
MhlI	GDGCHC	5 cut(s)	198, 234, 246, 923, 1339
MluCI	AATT	7 cut(s)	554, 566, 595, 1157, 1459, 1596, 1870
Mly113I	GGCGCC	1 cut(s)	530
MlyI	GAGTC	6 cut(s)	118, 195, 245, 940, 1801, 2143
MmeI	TCCRAC	5 cut(s)	24, 171, 223, 271, 1802
Mph1103I	ATGCAT	1 cut(s)	1774
MroXI	GAANNNNTTC	2 cut(s)	846, 1874
MslI	CAYNNNNRTG	1 cut(s)	1514
MspA1I	CMGCKG	3 cut(s)	830, 1168, 1413
MunI	CAATTG	1 cut(s)	1157
Mva1269I	GAATGC	1 cut(s)	391
MvaI	CCWGG	6 cut(s)	40, 350, 454, 936, 1259, 2298
MvnI	CGCG	4 cut(s)	206, 284, 377, 478
MwoI	GCNNNNNNNGC	9 cut(s)	145, 158, 395, 748, 893, 1586, 1769, 1949, 2293
NarI	GGCGCC	1 cut(s)	530
NciI	CCSGG	5 cut(s)	182, 252, 260, 1065, 1324
NcoI	CCATGG	1 cut(s)	94
NheI	GCTAGC	1 cut(s)	2093
NlaIII	CATG	6 cut(s)	98, 1007, 1232, 1539, 2255, 2287
NlaIV	GGNNCC	9 cut(s)	186, 245, 403, 531, 605, 886, 1327, 1378, 2112
NmeAIII	GCCGAG	1 cut(s)	471
NmuCI	GTSAC	5 cut(s)	695, 1392, 2033, 2083, 2154
NruI	TCGCGA	1 cut(s)	206
NsiI	ATGCAT	1 cut(s)	1774
PceI	AGGCCT	1 cut(s)	1610
PciSI	GCTCTTC	1 cut(s)	222
PcsI	WCGNNNNNNNCGW	1 cut(s)	716
PctI	GAATGC	1 cut(s)	391
PdmI	GAANNNNTTC	2 cut(s)	846, 1874
PfeI	GAWTC	6 cut(s)	67, 421, 791, 1487, 1646, 1886
PflMI	CCANNNNNTGG	2 cut(s)	95, 599
PfoI	TCCNGGA	1 cut(s)	934
PkrI	GCNGC	9 cut(s)	141, 160, 391, 480, 483, 1111, 1412, 1415, 1765
PleI	GAGTC	6 cut(s)	118, 195, 244, 939, 1801, 2142
PluTI	GGCGCC	1 cut(s)	533
PmaCI	CACGTG	1 cut(s)	526
PmlI	CACGTG	1 cut(s)	526
PpsI	GAGTC	6 cut(s)	118, 195, 244, 939, 1801, 2142
Ppu21I	YACGTR	1 cut(s)	526
PpuMI	RGGWCCY	1 cut(s)	961
PshAI	GACNNNNGTC	1 cut(s)	587
Psp124BI	GAGCTC	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	961
Psp6I	CCWGG	6 cut(s)	38, 348, 452, 934, 1257, 2296
PspCI	CACGTG	1 cut(s)	526
PspEI	GGTNACC	1 cut(s)	2154
PspFI	CCCAGC	2 cut(s)	896, 916
PspGI	CCWGG	6 cut(s)	38, 348, 452, 934, 1257, 2296
PspN4I	GGNNCC	9 cut(s)	186, 245, 403, 531, 605, 886, 1327, 1378, 2112
PspPI	GGNCC	7 cut(s)	184, 334, 346, 352, 961, 1326, 1602
PspPPI	RGGWCCY	1 cut(s)	961
PsrI	GAACNNNNNNTAC	2 cut(s)	1692, 1724
PstI	CTGCAG	1 cut(s)	855
PsuI	RGATCY	1 cut(s)	1364
PvuII	CAGCTG	2 cut(s)	830, 1413
RruI	TCGCGA	1 cut(s)	206
RsaI	GTAC	5 cut(s)	704, 826, 908, 1641, 1710
RsaNI	GTAC	5 cut(s)	703, 825, 907, 1640, 1709
RseI	CAYNNNNRTG	1 cut(s)	1514
SacI	GAGCTC	1 cut(s)	198
SapI	GCTCTTC	1 cut(s)	222
SatI	GCNGC	9 cut(s)	140, 159, 390, 479, 482, 1110, 1411, 1414, 1764
Sau96I	GGNCC	7 cut(s)	184, 334, 346, 352, 961, 1326, 1602
SchI	GAGTC	6 cut(s)	118, 195, 245, 940, 1801, 2143
SduI	GDGCHC	5 cut(s)	198, 234, 246, 923, 1339
SexAI	ACCWGGT	1 cut(s)	1257
SfaNI	GCATC	5 cut(s)	276, 766, 1258, 1425, 1781
SfcI	CTRYAG	2 cut(s)	851, 1682
SfoI	GGCGCC	1 cut(s)	531
SinI	GGWCC	4 cut(s)	184, 961, 1326, 1602
SmiMI	CAYNNNNRTG	1 cut(s)	1514
SmlI	CTYRAG	2 cut(s)	1481, 2098
SmoI	CTYRAG	2 cut(s)	1481, 2098
Sse9I	AATT	7 cut(s)	554, 566, 595, 1157, 1459, 1596, 1870
SseBI	AGGCCT	1 cut(s)	1610
SsiI	CCGC	9 cut(s)	156, 297, 389, 392, 478, 481, 495, 1168, 2202
SspDI	GGCGCC	1 cut(s)	529
SspMI	CTAG	6 cut(s)	1400, 1493, 1866, 2045, 2094, 2244
SstI	GAGCTC	1 cut(s)	198
StuI	AGGCCT	1 cut(s)	1610
StyI	CCWWGG	3 cut(s)	94, 1605, 1758
TaaI	ACNGT	6 cut(s)	472, 1045, 1360, 1513, 1697, 1936
TaiI	ACGT	6 cut(s)	318, 528, 702, 773, 908, 1723
TaqI	TCGA	6 cut(s)	65, 173, 461, 1456, 1585, 1705
TaqII	GACCGA	1 cut(s)	1380
TasI	AATT	7 cut(s)	554, 566, 595, 1157, 1459, 1596, 1870
TatI	WGTACW	3 cut(s)	702, 824, 1708
TauI	GCSGC	3 cut(s)	392, 481, 484
TfiI	GAWTC	6 cut(s)	67, 421, 791, 1487, 1646, 1886
TscAI	CASTG	4 cut(s)	1410, 1516, 1580, 2061
TseFI	GTSAC	5 cut(s)	695, 1392, 2033, 2083, 2154
TseI	GCWGC	6 cut(s)	139, 158, 1109, 1410, 1413, 1763
Tsp45I	GTSAC	5 cut(s)	695, 1392, 2033, 2083, 2154
TspDTI	ATGAA	8 cut(s)	128, 1001, 1053, 1161, 1224, 1292, 1923, 1982
TspGWI	ACGGA	5 cut(s)	46, 725, 1361, 1803, 2041
TspRI	CASTG	4 cut(s)	1410, 1516, 1580, 2061
Van91I	CCANNNNNTGG	2 cut(s)	95, 599
VpaK11BI	GGWCC	4 cut(s)	184, 961, 1326, 1602
XapI	RAATTY	2 cut(s)	566, 1459
XmnI	GAANNNNTTC	2 cut(s)	846, 1874
XspI	CTAG	6 cut(s)	1400, 1493, 1866, 2045, 2094, 2244
Zsp2I	ATGCAT	1 cut(s)	1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.