MD14G1227300.v1.1

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006760
Physical Location & Seq
Reverse (-)
30845997 .. 30850757
4761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1227300.v1.1.491

Sequence Viewer

Length: 2082 bp
ATGGCCGTGGCAAGCAAAACCAGAAATATGCTCGAGAATTTTGTAAGAGAAGGATCCTTCAAATGGTTGCTTGGAAATCGAAGCCCTTATGATGAAGAATTGGAGGAGATGGAAAGATCTCCTTCGGCCCGGACAAATTGGGTACCAGAGCTATCTCCAATTGCAAACATAGTAGTCCGAAGATGTTCAAAAATCCTTGGTGTTCCTACAACTGAGCTTTGTGAAGGCTTCAATTCTGAGGCTTCTGAATCTATAAAGCATCCATCGTGTTATGCAAGGAACTTTTTGGAATACTGCTGTTTTCGAGCACTTGCTCTATCCACTCAAGTAACAGGTCATCTGGCTGATAAAAAGTTTCGACGCCTGACATATGACATGATGATAGCTTGGGAGGCTCCAGCTGCTGCTAGCCAACCCTTACTAAATTTAGATGAGGATTTATCTGTTGGGGTAGAGGCTTTCTCTAGAATAGCTCCAGCAGTTCCTATTATTGCAAATGTGATTACCAGTGAAAATATTTTTAAGGTGCTTGCATCATCAAGCGATGGGAGACTTCAGTTCTTCACATACGACAAGTACCTAAGTGGACTAGAAAGAGCAATAAGAAAAATGAGGAGCCAATCAGAGTCGTCACTTCTTTCTGCCATGCGATCATCAAAAAGAGAGAAGATTCTGGAAGTGGATGGAACGGTCACTACCCAGCCAGTTCTTGAGCACGTAGGAATTTCTACATGGCCTGGAAGGTTAATTCTGACAGACCATGCACTTTACTTCGAGGCCCTGCGTGTTGTATCTTATGACAAGGCGAAACGATATGATCTATCAGATGACTTAAAACAGATTGTGAAACCCGAGTTGACTGGCCCTTGGGGTACTAGACTGTTTGACAAGGCAGTCTTTTATAAATCAGTTTCGTTATCAGAACCAGCTATAATAGAGTTCCCAGAGCTGAAGGGGCATACACGTCGTGATTATTGGCTAGCAATCATACGGGAGATTTTATATGTTCATCGATTTATATATAAATACCAGATCAAGGGGATTAAAAAGGATGAAGCACTTTCAAAGGCAGTGCTTGGAATTCTGCGACTACAAGCAATTAAAGAAATTAGCTCTCAAACTGATTTACGCTACGAGAGTCTTTTAATGTTCAATCTTTGTGATCAATTACCAGGTGGAGACTTGATACTTGAAACCATGGCAGATATGTCAACTTTTAAGGAATTAAATAGGTCTAGCAATTCTAAGCCCGGAGGTGGAATGTATTCGATCTCAGCTTTGGACATGATTTCTAACTTGGGATTTTCATTTGGAACAAGTTCTAGTAGTTCTGTTGAGGCTGGGCTTGCCGTGGGTGAGATAACTGTGGGAGAAGTTACTTTGCTTGAAAAAGCAGTTAAGGAATCTAAAAACAACTATGAAAAAGTGGCGCTAGCGCAAGCAACAGTAGATGGAGTCAAAGTGGAAGGGATCGATACAAATTTTGCAGTAATGAAGGAGTTACTCTTTCCAATGATGGAACTATGGACGTGCCTTCTTTCTTTGGCATTGTGGGAGGATCCGTTGAAGTCTTTGCTTTTCTGTTGTGTTTTCACTTACATCATTTGCAGGGGATGGCTGAGCTATGCCTTTGCGCTGACTCTCGTCTTTATTGCGGTCTTCATGGTCCTCACTCGATATTTTAGCCAAGGAAAGTCCACTCACGAGGTTAAGGTGTTAGCCCCTCCAGCGATGAATACGATGGAGCAGCTTTTGGCTGTTCAGAATGCAATATCTCAAGCTGAAGGGATCATCCAGGATGGGAACATTGTTCTTCTCAAGCTACGTGCCTTGTTGCTGTCCCTTTTTCCTCAGGCGAGTGAAAGATTCGCTATAGCTCTTCTAGTTATGGCTTTGGCTCTGGCGTTTCTGCCCGTTAAATACATTGTTCTATATATCTTTTTGGAGACGTTTACATGTTATTCGCCGGTGAGGAGATCTAGCACAGAGAGATGGACGAGAAGATTGCGAGAGTGGTGGTTCAGCATACCCGCGGCGCCCGTTATTCTTGAAAGAGAAAAAGAAGAGAAGAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

694

Amino Acids

78.18

Weight (kDa)

8.12

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 459 - 686 7.5e-84 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AasI GACNNNNNNGTC 1 cut(s) 893
Acc65I GGTACC 1 cut(s) 142
AccB1I GGYRCC 2 cut(s) 142, 2035
AccII CGCG 1 cut(s) 2033
AciI CCGC 3 cut(s) 1655, 2031, 2033
AclWI GGATC 6 cut(s) 48, 61, 1478, 1553, 1566, 1796
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 5 cut(s) 37, 424, 723, 1080, 1480
AcuI CTGAAG 3 cut(s) 539, 971, 1803
AcyI GRCGYC 2 cut(s) 361, 2036
AdeI CACNNNGTG 1 cut(s) 968
AfaI GTAC 3 cut(s) 144, 578, 874
AfiI CCNNNNNNNGG 3 cut(s) 63, 1036, 1256
AflIII ACRYGT 2 cut(s) 962, 1955
AgsI TTSAA 9 cut(s) 61, 189, 232, 1065, 1153, 1193, 1388, 1567, 2051
AjiI CACGTC 2 cut(s) 965, 1530
AjnI CCWGG 3 cut(s) 736, 1171, 1794
Alw21I GWGCWC 2 cut(s) 310, 717
Alw26I GTCTC 3 cut(s) 544, 1173, 1940
AlwI GGATC 6 cut(s) 48, 61, 1478, 1553, 1566, 1796
AlwNI CAGNNNCTG 1 cut(s) 404
Ama87I CYCGRG 2 cut(s) 32, 851
AoxI GGCC 5 cut(s) 3, 126, 734, 777, 862
ApeKI GCWGC 3 cut(s) 401, 404, 1747
ApoI RAATTY 5 cut(s) 37, 424, 723, 1080, 1480
Asp700I GAANNNNTTC 3 cut(s) 184, 1264, 1318
Asp718I GGTACC 1 cut(s) 142
AspLEI GCGC 4 cut(s) 1432, 1438, 1636, 2038
AspS9I GGNCC 4 cut(s) 127, 778, 863, 1666
AsuC2I CCSGG 2 cut(s) 130, 1251
AsuHPI GGTGA 2 cut(s) 1367, 1981
AsuNHI GCTAGC 3 cut(s) 407, 979, 1432
AvaI CYCGRG 2 cut(s) 32, 851
AvaII GGWCC 1 cut(s) 1666
AxyI CCTNAGG 1 cut(s) 1851
BamHI GGATCC 2 cut(s) 53, 1558
BanI GGYRCC 2 cut(s) 142, 2035
BauI CACGAG 1 cut(s) 1703
BbsI GAAGAC 1 cut(s) 1651
Bbv12I GWGCWC 2 cut(s) 310, 717
BbvI GCAGC 3 cut(s) 388, 391, 1759
BceAI ACGGC 1 cut(s) 1334
BcgI CGANNNNNNTGC 4 cut(s) 947, 981, 1987, 2021
BciT130I CCWGG 3 cut(s) 738, 1173, 1796
BclI TGATCA 1 cut(s) 1162
BcnI CCSGG 2 cut(s) 130, 1251
BcoDI GTCTC 3 cut(s) 544, 1173, 1940
BfmI CTRYAG 1 cut(s) 1872
BfoI RGCGCY 2 cut(s) 1433, 2039
BglII AGATCT 2 cut(s) 116, 1976
BisI GCNGC 4 cut(s) 402, 405, 1748, 2034
BlpI GCTNAGC 1 cut(s) 1619
BlsI GCNGC 4 cut(s) 403, 406, 1749, 2035
Bme1390I CCNGG 5 cut(s) 130, 738, 1173, 1251, 1796
Bme18I GGWCC 1 cut(s) 1666
BmeT110I CYCGRG 2 cut(s) 32, 851
BmgBI CACGTC 2 cut(s) 965, 1530
BmgT120I GGNCC 4 cut(s) 127, 778, 863, 1666
BmiI GGNNCC 6 cut(s) 55, 144, 396, 617, 1560, 2037
BmrFI CCNGG 5 cut(s) 130, 738, 1173, 1251, 1796
BmsI GCATC 2 cut(s) 268, 542
BmtI GCTAGC 3 cut(s) 411, 983, 1436
BoxI GACNNNNGTC 1 cut(s) 1643
BpiI GAAGAC 1 cut(s) 1651
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 3 cut(s) 381, 459, 1710
Bpu1102I GCTNAGC 1 cut(s) 1619
BpuEI CTTGAG 4 cut(s) 309, 731, 1761, 1802
BpuMI CCSGG 2 cut(s) 130, 1251
Bsa29I ATCGAT 2 cut(s) 1012, 1473
BsaAI YACGTR 2 cut(s) 718, 1826
BsaHI GRCGYC 2 cut(s) 361, 2036
BsaJI CCNNGG 7 cut(s) 6, 196, 866, 1197, 1350, 1687, 2031
BsaXI ACNNNNNCTCC 4 cut(s) 1446, 1476, 1547, 1577
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 1036, 1256
Bse118I RCCGGY 1 cut(s) 1966
Bse1I ACTGG 3 cut(s) 507, 704, 865
Bse21I CCTNAGG 1 cut(s) 1851
BseBI CCWGG 3 cut(s) 738, 1173, 1796
BseCI ATCGAT 2 cut(s) 1012, 1473
BseDI CCNNGG 7 cut(s) 6, 196, 866, 1197, 1350, 1687, 2031
BseGI GGATG 6 cut(s) 259, 688, 1057, 1619, 1791, 1804
BseLI CCNNNNNNNGG 3 cut(s) 63, 1036, 1256
BseMII CTCAG 5 cut(s) 204, 228, 1287, 1610, 1865
BseNI ACTGG 3 cut(s) 507, 704, 865
BseRI GAGGAG 3 cut(s) 119, 628, 1987
BseXI GCAGC 3 cut(s) 388, 391, 1759
BseYI CCCAGC 2 cut(s) 699, 1340
Bsh1236I CGCG 1 cut(s) 2033
BshFI GGCC 5 cut(s) 5, 128, 736, 779, 864
BshNI GGYRCC 2 cut(s) 142, 2035
BshVI ATCGAT 2 cut(s) 1012, 1473
BsiHKAI GWGCWC 2 cut(s) 310, 717
BsiHKCI CYCGRG 2 cut(s) 32, 851
BsiSI CCGG 3 cut(s) 130, 1251, 1967
BslFI GGGAC 1 cut(s) 1825
BslI CCNNNNNNNGG 3 cut(s) 63, 1036, 1256
BsmAI GTCTC 3 cut(s) 544, 1173, 1940
BsmBI CGTCTC 1 cut(s) 1940
BsmFI GGGAC 1 cut(s) 1825
BsmI GAATGC 1 cut(s) 1771
BsnI GGCC 5 cut(s) 5, 128, 736, 779, 864
BsoBI CYCGRG 2 cut(s) 32, 851
Bsp1286I GDGCHC 2 cut(s) 310, 717
Bsp1720I GCTNAGC 1 cut(s) 1619
Bsp19I CCATGG 1 cut(s) 1197
BspACI CCGC 3 cut(s) 1655, 2031, 2033
BspANI GGCC 5 cut(s) 5, 128, 736, 779, 864
BspCNI CTCAG 5 cut(s) 205, 229, 1286, 1611, 1864
BspDI ATCGAT 2 cut(s) 1012, 1473
BspFNI CGCG 1 cut(s) 2033
BspLI GGNNCC 6 cut(s) 55, 144, 396, 617, 1560, 2037
BspOI GCTAGC 3 cut(s) 411, 983, 1436
BspPI GGATC 6 cut(s) 48, 61, 1478, 1553, 1566, 1796
BspQI GCTCTTC 1 cut(s) 1884
BspT107I GGYRCC 2 cut(s) 142, 2035
BsrFI RCCGGY 1 cut(s) 1966
BsrI ACTGG 3 cut(s) 507, 704, 865
BssAI RCCGGY 1 cut(s) 1966
BssECI CCNNGG 7 cut(s) 6, 196, 866, 1197, 1350, 1687, 2031
BssNI GRCGYC 2 cut(s) 361, 2036
BssSI CACGAG 1 cut(s) 1703
BssT1I CCWWGG 4 cut(s) 196, 866, 1197, 1687
Bst2BI CACGAG 1 cut(s) 1703
Bst2UI CCWGG 3 cut(s) 738, 1173, 1796
Bst4CI ACNGT 4 cut(s) 691, 882, 1366, 1447
Bst6I CTCTTC 2 cut(s) 1884, 2058
BstACI GRCGYC 2 cut(s) 361, 2036
BstBAI YACGTR 2 cut(s) 718, 1826
BstC8I GCNNGC 7 cut(s) 13, 409, 531, 981, 1347, 1434, 1440
BstDEI CTNAG 7 cut(s) 213, 237, 581, 1245, 1273, 1619, 1851
BstDSI CCRYGG 4 cut(s) 6, 1197, 1350, 2031
BstF5I GGATG 6 cut(s) 259, 688, 1057, 1619, 1791, 1804
BstFNI CGCG 1 cut(s) 2033
BstH2I RGCGCY 2 cut(s) 1433, 2039
BstHHI GCGC 4 cut(s) 1432, 1438, 1636, 2038
BstMAI GTCTC 3 cut(s) 544, 1173, 1940
BstMWI GCNNNNNNNGC 5 cut(s) 392, 401, 955, 1346, 1727
BstNI CCWGG 3 cut(s) 738, 1173, 1796
BstNSI RCATGY 1 cut(s) 1959
BstPAI GACNNNNGTC 1 cut(s) 1643
BstSCI CCNGG 5 cut(s) 128, 736, 1171, 1249, 1794
BstSFI CTRYAG 1 cut(s) 1872
BstUI CGCG 1 cut(s) 2033
BstV1I GCAGC 3 cut(s) 388, 391, 1759
BstV2I GAAGAC 1 cut(s) 1651
BstX2I RGATCY 4 cut(s) 53, 116, 1558, 1976
BstYI RGATCY 4 cut(s) 53, 116, 1558, 1976
Bsu15I ATCGAT 2 cut(s) 1012, 1473
Bsu36I CCTNAGG 1 cut(s) 1851
BsuRI GGCC 5 cut(s) 5, 128, 736, 779, 864
BsuTUI ATCGAT 2 cut(s) 1012, 1473
BtgI CCRYGG 4 cut(s) 6, 1197, 1350, 2031
BtgZI GCGATG 2 cut(s) 558, 1745
BtrI CACGTC 2 cut(s) 965, 1530
BtsCI GGATG 6 cut(s) 259, 688, 1057, 1619, 1791, 1804
BtsI GCAGTG 1 cut(s) 1077
BtsIMutI CAGTG 2 cut(s) 514, 1077
Cac8I GCNNGC 7 cut(s) 13, 409, 531, 981, 1347, 1434, 1440
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 4 cut(s) 1432, 1438, 1636, 2038
Cfr10I RCCGGY 1 cut(s) 1966
Cfr13I GGNCC 4 cut(s) 127, 778, 863, 1666
Cfr42I CCGCGG 1 cut(s) 2034
ClaI ATCGAT 2 cut(s) 1012, 1473
CseI GACGC 1 cut(s) 369
CsiI ACCWGGT 1 cut(s) 1171
Csp6I GTAC 3 cut(s) 143, 577, 873
CviAII CATG 8 cut(s) 376, 646, 732, 761, 1198, 1285, 1663, 1956
CviQI GTAC 3 cut(s) 143, 577, 873
DdeI CTNAG 7 cut(s) 213, 237, 581, 1245, 1273, 1619, 1851
DinI GGCGCC 1 cut(s) 2037
DraIII CACNNNGTG 1 cut(s) 968
DrdI GACNNNNNNGTC 1 cut(s) 893
DseDI GACNNNNNNGTC 1 cut(s) 893
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 1884, 2058
EarI CTCTTC 2 cut(s) 1884, 2058
Eco130I CCWWGG 4 cut(s) 196, 866, 1197, 1687
Eco47I GGWCC 1 cut(s) 1666
Eco57I CTGAAG 3 cut(s) 539, 971, 1803
Eco81I CCTNAGG 1 cut(s) 1851
Eco88I CYCGRG 2 cut(s) 32, 851
EcoO109I RGGNCCY 1 cut(s) 778
EcoRI GAATTC 1 cut(s) 1080
EcoRII CCWGG 3 cut(s) 736, 1171, 1794
EcoT14I CCWWGG 4 cut(s) 196, 866, 1197, 1687
EgeI GGCGCC 1 cut(s) 2037
EheI GGCGCC 1 cut(s) 2037
ErhI CCWWGG 4 cut(s) 196, 866, 1197, 1687
Esp3I CGTCTC 1 cut(s) 1940
FaeI CATG 8 cut(s) 379, 649, 735, 764, 1201, 1288, 1666, 1959
FalI AAGNNNNNCTT 4 cut(s) 106, 138, 881, 913
FaqI GGGAC 1 cut(s) 1825
FatI CATG 8 cut(s) 375, 645, 731, 760, 1197, 1284, 1662, 1955
FauI CCCGC 1 cut(s) 2038
FauNDI CATATG 1 cut(s) 370
FbaI TGATCA 1 cut(s) 1162
Fnu4HI GCNGC 4 cut(s) 402, 405, 1748, 2034
FokI GGATG 6 cut(s) 246, 695, 1064, 1626, 1778, 1811
Fsp4HI GCNGC 4 cut(s) 402, 405, 1748, 2034
GlaI GCGC 4 cut(s) 1431, 1437, 1635, 2037
GluI GCNGC 4 cut(s) 402, 405, 1748, 2034
GsaI CCCAGC 2 cut(s) 703, 1344
GsuI CTGGAG 3 cut(s) 381, 459, 1710
HaeII RGCGCY 2 cut(s) 1433, 2039
HaeIII GGCC 5 cut(s) 5, 128, 736, 779, 864
HapII CCGG 3 cut(s) 130, 1251, 1967
HgaI GACGC 1 cut(s) 369
HhaI GCGC 4 cut(s) 1432, 1438, 1636, 2038
Hin1I GRCGYC 2 cut(s) 361, 2036
Hin1II CATG 8 cut(s) 379, 649, 735, 764, 1201, 1288, 1666, 1959
Hin6I GCGC 4 cut(s) 1430, 1436, 1634, 2036
HinP1I GCGC 4 cut(s) 1430, 1436, 1634, 2036
HincII GTYRAC 2 cut(s) 858, 1212
HindII GTYRAC 2 cut(s) 858, 1212
HinfI GANTC 8 cut(s) 248, 626, 670, 1138, 1403, 1455, 1639, 1866
HpaII CCGG 3 cut(s) 130, 1251, 1967
HphI GGTGA 2 cut(s) 1367, 1981
Hpy166II GTNNAC 5 cut(s) 587, 858, 1212, 1698, 1953
Hpy188I TCNGA 8 cut(s) 179, 238, 247, 625, 753, 826, 922, 1764
Hpy188III TCNNGA 7 cut(s) 34, 465, 674, 710, 968, 1703, 2048
Hpy8I GTNNAC 5 cut(s) 587, 858, 1212, 1698, 1953
Hpy99I CGWCG 2 cut(s) 363, 969
HpyCH4III ACNGT 4 cut(s) 691, 882, 1366, 1447
HpyCH4IV ACGT 5 cut(s) 717, 964, 1529, 1825, 1949
HpyCH4V TGCA 8 cut(s) 164, 275, 494, 533, 764, 1487, 1608, 1769
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 401, 955, 1346, 1727
HpyF3I CTNAG 7 cut(s) 213, 237, 581, 1245, 1273, 1619, 1851
HpySE526I ACGT 5 cut(s) 717, 964, 1529, 1825, 1949
Hsp92I GRCGYC 2 cut(s) 361, 2036
Hsp92II CATG 8 cut(s) 379, 649, 735, 764, 1201, 1288, 1666, 1959
HspAI GCGC 4 cut(s) 1430, 1436, 1634, 2036
KasI GGCGCC 1 cut(s) 2035
KpnI GGTACC 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 1162
KspI CCGCGG 1 cut(s) 2034
LguI GCTCTTC 1 cut(s) 1884
LmnI GCTCC 4 cut(s) 400, 478, 615, 1744
Lsp1109I GCAGC 3 cut(s) 388, 391, 1759
LweI GCATC 2 cut(s) 268, 542
MabI ACCWGGT 1 cut(s) 1171
MaeII ACGT 5 cut(s) 717, 964, 1529, 1825, 1949
MaeIII GTNAC 5 cut(s) 328, 630, 691, 1375, 1500
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 4 cut(s) 53, 116, 1558, 1976
MhlI GDGCHC 2 cut(s) 310, 717
Mly113I GGCGCC 1 cut(s) 2036
MlyI GAGTC 4 cut(s) 635, 1147, 1464, 1633
MroXI GAANNNNTTC 3 cut(s) 184, 1264, 1318
MspA1I CMGCKG 2 cut(s) 401, 2033
MspI CCGG 3 cut(s) 130, 1251, 1967
MspR9I CCNGG 5 cut(s) 130, 738, 1173, 1251, 1796
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 1771
MvaI CCWGG 3 cut(s) 738, 1173, 1796
MvnI CGCG 1 cut(s) 2033
MwoI GCNNNNNNNGC 5 cut(s) 392, 401, 955, 1346, 1727
NarI GGCGCC 1 cut(s) 2036
NciI CCSGG 2 cut(s) 130, 1251
NcoI CCATGG 1 cut(s) 1197
NdeI CATATG 1 cut(s) 370
NheI GCTAGC 3 cut(s) 407, 979, 1432
NlaIII CATG 8 cut(s) 379, 649, 735, 764, 1201, 1288, 1666, 1959
NlaIV GGNNCC 6 cut(s) 55, 144, 396, 617, 1560, 2037
NmuCI GTSAC 2 cut(s) 630, 691
NspI RCATGY 1 cut(s) 1959
PaeR7I CTCGAG 1 cut(s) 32
PciI ACATGT 1 cut(s) 1955
PciSI GCTCTTC 1 cut(s) 1884
PctI GAATGC 1 cut(s) 1771
PdmI GAANNNNTTC 3 cut(s) 184, 1264, 1318
PfeI GAWTC 4 cut(s) 248, 670, 1403, 1866
PfoI TCCNGGA 1 cut(s) 1794
PkrI GCNGC 4 cut(s) 403, 406, 1749, 2035
PleI GAGTC 4 cut(s) 634, 1146, 1463, 1633
PluTI GGCGCC 1 cut(s) 2039
PpsI GAGTC 4 cut(s) 634, 1146, 1463, 1633
Ppu21I YACGTR 2 cut(s) 718, 1826
PscI ACATGT 1 cut(s) 1955
PshAI GACNNNNGTC 1 cut(s) 1643
PsiI TTATAA 1 cut(s) 903
Psp6I CCWGG 3 cut(s) 736, 1171, 1794
PspFI CCCAGC 2 cut(s) 699, 1340
PspGI CCWGG 3 cut(s) 736, 1171, 1794
PspN4I GGNNCC 6 cut(s) 55, 144, 396, 617, 1560, 2037
PspPI GGNCC 4 cut(s) 127, 778, 863, 1666
PsrI GAACNNNNNNTAC 2 cut(s) 679, 711
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 4 cut(s) 53, 116, 1558, 1976
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 3 cut(s) 144, 578, 874
RsaNI GTAC 3 cut(s) 143, 577, 873
SacII CCGCGG 1 cut(s) 2034
SapI GCTCTTC 1 cut(s) 1884
SatI GCNGC 4 cut(s) 402, 405, 1748, 2034
Sau96I GGNCC 4 cut(s) 127, 778, 863, 1666
SchI GAGTC 4 cut(s) 635, 1147, 1464, 1633
ScrFI CCNGG 5 cut(s) 130, 738, 1173, 1251, 1796
SduI GDGCHC 2 cut(s) 310, 717
SexAI ACCWGGT 1 cut(s) 1171
SfaNI GCATC 2 cut(s) 268, 542
SfcI CTRYAG 1 cut(s) 1872
SfoI GGCGCC 1 cut(s) 2037
Sfr274I CTCGAG 1 cut(s) 32
Sfr303I CCGCGG 1 cut(s) 2034
SgrAI CRCCGGYG 1 cut(s) 1966
SgrBI CCGCGG 1 cut(s) 2034
SinI GGWCC 1 cut(s) 1666
SlaI CTCGAG 1 cut(s) 32
SmlI CTYRAG 5 cut(s) 32, 324, 710, 1776, 1817
SmoI CTYRAG 5 cut(s) 32, 324, 710, 1776, 1817
SsiI CCGC 3 cut(s) 1655, 2031, 2033
SspDI GGCGCC 1 cut(s) 2035
SspI AATATT 1 cut(s) 517
StyD4I CCNGG 5 cut(s) 128, 736, 1171, 1249, 1794
StyI CCWWGG 4 cut(s) 196, 866, 1197, 1687
TaaI ACNGT 4 cut(s) 691, 882, 1366, 1447
TaiI ACGT 5 cut(s) 720, 967, 1532, 1828, 1952
TaqI TCGA 9 cut(s) 33, 79, 304, 358, 774, 1012, 1268, 1473, 1675
TauI GCSGC 1 cut(s) 2036
TfiI GAWTC 4 cut(s) 248, 670, 1403, 1866
TscAI CASTG 2 cut(s) 514, 1077
TseFI GTSAC 2 cut(s) 630, 691
TseI GCWGC 3 cut(s) 401, 404, 1747
Tsp45I GTSAC 2 cut(s) 630, 691
TspDTI ATGAA 8 cut(s) 108, 998, 1068, 1296, 1434, 1508, 1651, 1748
TspGWI ACGGA 1 cut(s) 1551
TspRI CASTG 2 cut(s) 514, 1077
VpaK11BI GGWCC 1 cut(s) 1666
XapI RAATTY 5 cut(s) 37, 424, 723, 1080, 1480
XbaI TCTAGA 1 cut(s) 464
XceI RCATGY 1 cut(s) 1959
XhoI CTCGAG 1 cut(s) 32
XmnI GAANNNNTTC 3 cut(s) 184, 1264, 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.