RLG00000004901

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006760
Physical Location & Seq
Forward (+)
63535052 .. 63540842
5791 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004901

Sequence Viewer

Length: 1611 bp
ATGGCGGTGGCAAGCAAAACCAGAAATATGATCGAGAATCTTGTAAGAGAAGGATCGTTCAAATGGTTGATTGGAAATCGAAGCCCTTTTGATGACGAATTGGAGGAGATGGAGAGGTCTCCTTCAGCCCCAACAAATTGGATACCGGAGCTATCTCCTATTGCAAATGTAGTTGTCCGTAGATGCTCCAAAATCCTTGGTGTTCCTACGACTGAGCTTTGTAAAGGCTTCAGTGCAGAGGCTTCCGAGTCTATAAAGCATCCATCTCGCTATGCAAGGAACTTTTTGGAATACTGCTCTTTCAGAGCTCTCGCTCTGTCAACTCAGGTGACAGGTCATCTGGCTGATAAAAAGTTTCGACGCCTAACATATGACATGATGCTAGCTTGGGAGGCTCCAGCTTCTGGCAGCCAACCTTTGCTAAATATAAATGAGGATTTATCAGTTGGGATAGAGGCTTTCTCTAGAATAGCTCCGTCAGTTCCCATTATTGCAAATGTGATTATCAGCGAAAATCTTTTTGAGGTACTTGCAACATCAACTGGTGGGAGACTTCAGTTCTCCACTTATGACAAGTATCTAAGTGGACTGGAAAGAGCAATAAGAAAAATGAGGACCCAATCAGAGTCATCTCTTCTTTCTGCTGTACGATCATCAAGAGGAGAGAGAATTCTGGAAGTGGATGGAACAGTAACTACACAACCAGTTCTTGAGCATGTGGGAATTTCTACATGGCCTGGACGGTTGATTCTGACAGACCATGCACTTTACTTTGAGGCTCTGCGTGTTGTATCTTATGACAAGGCAAAACGATATGACCTATCAGATGACCTAAATCAGGTCGTGAAACCTGAGTTGACTGGCCCATGGGGTACTAGACTGTTTGACAAGGCAGTCTTTTATAAATCAATTTCATTATCAGAACCAGTTGTGATAGAGTTTCCTGAGCTGAAGGGGCATTCTCGTCGTGATTACTGGCTAGCGATCATAAGAGAGATTTTGTATGTTCACAGATTTATACATAAATACCAAATCAAGGGGGTCAAACGAGATGAAGCGCTATCAAAGGCTGTGCTTGGGATTCTGCGAGTACAAGCCATTCAAGAAATTTCTTCGGCTCCTTTACGCTCTGAGGGTCTTTTAATGTTCAATCTTTGTGATCAATTACCAGGTGGAGACTTGATACTGGAAACTCTTGCAAATATGTCAACCGTAAGCGAATTAAATAGGTCTGACACCGCTAAGTCTGGAGGTGGAATGTATTCGATCTCAGCATTAGATATGATTTCTAACTTGGGCTTCGCATTTGGAACAAGTTCTAGTAATTCGGTTGAGGCTGGTCTTGCTGTGGGTGAGGTAACTGTTGGACAACTGACTTCTCTGGAAAGAGCAGTTAAGGAATCAAAAAACAATTACGAAAAAGTGGCACAAGCACAAGCAACAGTAGATGGAGTCAAAGTGGAAGGCATTGATACGAATGTTGCAGTGATGAAGGAGTTGCTCTTTCCAATGGTGGAACTAGGGAAATGGCTTCTTTCTTTGGCATTATGGGAGGATCCACTGAAGTCTCTGGGTTTCTGTGTTGTTTTCACTTACATCATATGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

537

Amino Acids

59.49

Weight (kDa)

6.26

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 458 - 536 2.4e-23 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AasI GACNNNNNNGTC 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 1596
AccB7I CCANNNNNTGG 1 cut(s) 404
AciI CCGC 2 cut(s) 5, 1239
AclWI GGATC 3 cut(s) 61, 1550, 1563
AcsI RAATTY 3 cut(s) 669, 723, 1107
AcuI CTGAAG 5 cut(s) 108, 214, 539, 971, 1583
AcyI GRCGYC 1 cut(s) 361
AfaI GTAC 4 cut(s) 528, 648, 874, 1092
AfeI AGCGCT 1 cut(s) 1059
AfiI CCNNNNNNNGG 3 cut(s) 404, 838, 1036
AgsI TTSAA 3 cut(s) 61, 1103, 1150
AjnI CCWGG 2 cut(s) 736, 1168
AluBI AGCT 7 cut(s) 151, 217, 308, 386, 401, 473, 949
AluI AGCT 7 cut(s) 151, 217, 308, 386, 401, 473, 949
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 4 cut(s) 123, 544, 1170, 1572
AlwI GGATC 3 cut(s) 61, 1550, 1563
AlwNI CAGNNNCTG 1 cut(s) 404
Aor51HI AGCGCT 1 cut(s) 1059
AoxI GGCC 2 cut(s) 734, 862
ApeKI GCWGC 1 cut(s) 408
ApoI RAATTY 3 cut(s) 669, 723, 1107
Asp700I GAANNNNTTC 2 cut(s) 1261, 1315
AspLEI GCGC 1 cut(s) 1060
AspS9I GGNCC 2 cut(s) 615, 863
AsuHPI GGTGA 2 cut(s) 340, 1364
AsuNHI GCTAGC 2 cut(s) 382, 979
AvaII GGWCC 1 cut(s) 615
BaeI ACNNNNGTAYC 2 cut(s) 1464, 1497
BamHI GGATCC 1 cut(s) 1555
BanII GRGCYC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 1 cut(s) 420
BccI CCATC 4 cut(s) 103, 271, 677, 1442
BcgI CGANNNNNNTGC 4 cut(s) 248, 282, 947, 981
BciT130I CCWGG 2 cut(s) 738, 1170
BciVI GTATCC 1 cut(s) 135
BclI TGATCA 1 cut(s) 1159
BcoDI GTCTC 4 cut(s) 123, 544, 1170, 1572
BfaI CTAG 6 cut(s) 383, 465, 876, 980, 1320, 1520
BfoI RGCGCY 1 cut(s) 1061
BfuAI ACCTGC 1 cut(s) 1596
BfuI GTATCC 1 cut(s) 135
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
Bme1390I CCNGG 2 cut(s) 738, 1170
Bme18I GGWCC 1 cut(s) 615
BmgT120I GGNCC 2 cut(s) 615, 863
BmiI GGNNCC 4 cut(s) 396, 617, 1119, 1557
BmrFI CCNGG 2 cut(s) 738, 1170
BmsI GCATC 3 cut(s) 173, 268, 369
BmtI GCTAGC 2 cut(s) 386, 983
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 2 cut(s) 381, 1269
Bpu10I CCTNAGC 1 cut(s) 945
BpuEI CTTGAG 1 cut(s) 731
BsaHI GRCGYC 1 cut(s) 361
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 196, 866
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 2 cut(s) 1443, 1473
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 838, 1036
Bse1I ACTGG 7 cut(s) 547, 594, 704, 865, 926, 980, 1191
BseBI CCWGG 2 cut(s) 738, 1170
BseDI CCNNGG 2 cut(s) 196, 866
BseGI GGATG 2 cut(s) 259, 688
BseLI CCNNNNNNNGG 3 cut(s) 404, 838, 1036
BseMII CTCAG 6 cut(s) 204, 338, 843, 936, 1122, 1284
BseNI ACTGG 7 cut(s) 547, 594, 704, 865, 926, 980, 1191
BseRI GAGGAG 2 cut(s) 119, 675
BseXI GCAGC 1 cut(s) 420
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 2 cut(s) 736, 864
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 3 cut(s) 404, 838, 1036
BsmAI GTCTC 4 cut(s) 123, 544, 1170, 1572
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 2 cut(s) 736, 864
Bso31I GGTCTC 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 2 cut(s) 5, 1239
BspANI GGCC 2 cut(s) 736, 864
BspCNI CTCAG 6 cut(s) 205, 337, 844, 937, 1123, 1283
BspLI GGNNCC 4 cut(s) 396, 617, 1119, 1557
BspMI ACCTGC 1 cut(s) 1596
BspOI GCTAGC 2 cut(s) 386, 983
BspPI GGATC 3 cut(s) 61, 1550, 1563
BspTNI GGTCTC 1 cut(s) 123
BsrI ACTGG 7 cut(s) 547, 594, 704, 865, 926, 980, 1191
BssECI CCNNGG 2 cut(s) 196, 866
BssMI GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
BssNI GRCGYC 1 cut(s) 361
BssT1I CCWWGG 2 cut(s) 196, 866
Bst2UI CCWGG 2 cut(s) 738, 1170
Bst4CI ACNGT 6 cut(s) 691, 744, 882, 1213, 1363, 1444
Bst6I CTCTTC 1 cut(s) 639
BstACI GRCGYC 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 13, 384, 981
BstDEI CTNAG 8 cut(s) 213, 324, 581, 852, 945, 1131, 1242, 1270
BstDSI CCRYGG 1 cut(s) 866
BstENI CCTNNNNNAGG 1 cut(s) 836
BstF5I GGATG 2 cut(s) 259, 688
BstH2I RGCGCY 1 cut(s) 1061
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 7 cut(s) 33, 56, 653, 987, 1162, 1269, 1558
BstMAI GTCTC 4 cut(s) 123, 544, 1170, 1572
BstMBI GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
BstMWI GCNNNNNNNGC 3 cut(s) 392, 955, 1343
BstNI CCWGG 2 cut(s) 738, 1170
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 2 cut(s) 736, 1168
BstV1I GCAGC 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 1555
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 1 cut(s) 1555
BsuI GTATCC 1 cut(s) 135
BsuRI GGCC 2 cut(s) 736, 864
BtgI CCRYGG 1 cut(s) 866
BtsCI GGATG 2 cut(s) 259, 688
BtsI GCAGTG 1 cut(s) 1491
BtsIMutI CAGTG 3 cut(s) 238, 1491, 1559
BveI ACCTGC 1 cut(s) 1596
Cac8I GCNNGC 3 cut(s) 13, 384, 981
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 1 cut(s) 1060
Cfr13I GGNCC 2 cut(s) 615, 863
CseI GACGC 1 cut(s) 369
CsiI ACCWGGT 1 cut(s) 1168
Csp6I GTAC 4 cut(s) 527, 647, 873, 1091
CviAII CATG 5 cut(s) 376, 716, 732, 761, 867
CviQI GTAC 4 cut(s) 527, 647, 873, 1091
DdeI CTNAG 8 cut(s) 213, 324, 581, 852, 945, 1131, 1242, 1270
DpnI GATC 7 cut(s) 32, 55, 652, 986, 1161, 1268, 1557
DpnII GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
DrdI GACNNNNNNGTC 1 cut(s) 893
DseDI GACNNNNNNGTC 1 cut(s) 893
Eam1104I CTCTTC 1 cut(s) 639
EarI CTCTTC 1 cut(s) 639
Ecl136II GAGCTC 1 cut(s) 308
Eco130I CCWWGG 2 cut(s) 196, 866
Eco24I GRGCYC 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 615
Eco47III AGCGCT 1 cut(s) 1059
Eco53kI GAGCTC 1 cut(s) 308
Eco57I CTGAAG 5 cut(s) 108, 214, 539, 971, 1583
EcoICRI GAGCTC 1 cut(s) 308
EcoNI CCTNNNNNAGG 1 cut(s) 836
EcoO109I RGGNCCY 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 669
EcoRII CCWGG 2 cut(s) 736, 1168
EcoT14I CCWWGG 2 cut(s) 196, 866
EcoT38I GRGCYC 1 cut(s) 310
ErhI CCWWGG 2 cut(s) 196, 866
FaeI CATG 5 cut(s) 379, 719, 735, 764, 870
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FatI CATG 5 cut(s) 375, 715, 731, 760, 866
FauNDI CATATG 2 cut(s) 370, 1601
FbaI TGATCA 1 cut(s) 1159
Fnu4HI GCNGC 1 cut(s) 409
FokI GGATG 2 cut(s) 246, 695
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 1 cut(s) 409
FspBI CTAG 6 cut(s) 383, 465, 876, 980, 1320, 1520
GlaI GCGC 1 cut(s) 1059
GluI GCNGC 1 cut(s) 409
GsuI CTGGAG 2 cut(s) 381, 1269
HaeII RGCGCY 1 cut(s) 1061
HaeIII GGCC 2 cut(s) 736, 864
HapII CCGG 1 cut(s) 146
HgaI GACGC 1 cut(s) 369
HhaI GCGC 1 cut(s) 1060
Hin1I GRCGYC 1 cut(s) 361
Hin1II CATG 5 cut(s) 379, 719, 735, 764, 870
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HincII GTYRAC 3 cut(s) 321, 858, 1209
HindII GTYRAC 3 cut(s) 321, 858, 1209
HinfI GANTC 7 cut(s) 37, 248, 626, 748, 1081, 1400, 1452
HpaII CCGG 1 cut(s) 146
HphI GGTGA 2 cut(s) 340, 1364
Hpy166II GTNNAC 5 cut(s) 321, 587, 858, 1009, 1209
Hpy188I TCNGA 8 cut(s) 247, 305, 625, 753, 826, 922, 1132, 1234
Hpy8I GTNNAC 5 cut(s) 321, 587, 858, 1009, 1209
Hpy99I CGWCG 2 cut(s) 363, 969
HpyAV CCTTC 5 cut(s) 44, 132, 946, 1457, 1486
HpyCH4III ACNGT 6 cut(s) 691, 744, 882, 1213, 1363, 1444
HpyCH4V TGCA 9 cut(s) 164, 236, 275, 494, 533, 764, 1199, 1484, 1605
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 955, 1343
HpyF3I CTNAG 8 cut(s) 213, 324, 581, 852, 945, 1131, 1242, 1270
Hsp92I GRCGYC 1 cut(s) 361
Hsp92II CATG 5 cut(s) 379, 719, 735, 764, 870
HspAI GCGC 1 cut(s) 1058
Ksp22I TGATCA 1 cut(s) 1159
Kzo9I GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
LmnI GCTCC 5 cut(s) 148, 191, 400, 478, 1123
Lsp1109I GCAGC 1 cut(s) 420
LweI GCATC 3 cut(s) 173, 268, 369
MabI ACCWGGT 1 cut(s) 1168
MaeI CTAG 6 cut(s) 383, 465, 876, 980, 1320, 1520
MaeIII GTNAC 3 cut(s) 328, 691, 1357
MalI GATC 7 cut(s) 32, 55, 652, 986, 1161, 1268, 1557
MboI GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
MboII GAAGA 2 cut(s) 626, 1104
MflI RGATCY 1 cut(s) 1555
MhlI GDGCHC 1 cut(s) 310
MlyI GAGTC 3 cut(s) 257, 635, 1461
MmeI TCCRAC 1 cut(s) 1345
MroXI GAANNNNTTC 2 cut(s) 1261, 1315
MseI TTAA 3 cut(s) 1142, 1223, 1395
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 738, 1170
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 2 cut(s) 738, 1170
MwoI GCNNNNNNNGC 3 cut(s) 392, 955, 1343
NcoI CCATGG 1 cut(s) 866
NdeI CATATG 2 cut(s) 370, 1601
NdeII GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
NheI GCTAGC 2 cut(s) 382, 979
NlaIII CATG 5 cut(s) 379, 719, 735, 764, 870
NlaIV GGNNCC 4 cut(s) 396, 617, 1119, 1557
NmuCI GTSAC 1 cut(s) 328
NspI RCATGY 1 cut(s) 719
PctI GAATGC 1 cut(s) 958
PdmI GAANNNNTTC 2 cut(s) 1261, 1315
PfeI GAWTC 4 cut(s) 37, 748, 1081, 1400
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 1 cut(s) 410
PleI GAGTC 3 cut(s) 256, 634, 1460
PpsI GAGTC 3 cut(s) 256, 634, 1460
PpuMI RGGWCCY 1 cut(s) 615
PsiI TTATAA 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 615
Psp6I CCWGG 2 cut(s) 736, 1168
PspGI CCWGG 2 cut(s) 736, 1168
PspN4I GGNNCC 4 cut(s) 396, 617, 1119, 1557
PspPI GGNCC 2 cut(s) 615, 863
PspPPI RGGWCCY 1 cut(s) 615
PsrI GAACNNNNNNTAC 2 cut(s) 679, 711
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 1555
RsaI GTAC 4 cut(s) 528, 648, 874, 1092
RsaNI GTAC 4 cut(s) 527, 647, 873, 1091
SacI GAGCTC 1 cut(s) 310
SaqAI TTAA 3 cut(s) 1142, 1223, 1395
SatI GCNGC 1 cut(s) 409
Sau3AI GATC 7 cut(s) 30, 53, 650, 984, 1159, 1266, 1555
Sau96I GGNCC 2 cut(s) 615, 863
SchI GAGTC 3 cut(s) 257, 635, 1461
ScrFI CCNGG 2 cut(s) 738, 1170
SduI GDGCHC 1 cut(s) 310
SexAI ACCWGGT 1 cut(s) 1168
SfaNI GCATC 3 cut(s) 173, 268, 369
SinI GGWCC 1 cut(s) 615
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SsiI CCGC 2 cut(s) 5, 1239
SspMI CTAG 6 cut(s) 383, 465, 876, 980, 1320, 1520
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 2 cut(s) 736, 1168
StyI CCWWGG 2 cut(s) 196, 866
TaaI ACNGT 6 cut(s) 691, 744, 882, 1213, 1363, 1444
TaqI TCGA 4 cut(s) 33, 79, 358, 1265
TatI WGTACW 1 cut(s) 1090
TfiI GAWTC 4 cut(s) 37, 748, 1081, 1400
Tru1I TTAA 3 cut(s) 1142, 1223, 1395
Tru9I TTAA 3 cut(s) 1142, 1223, 1395
TscAI CASTG 3 cut(s) 238, 1491, 1566
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 1 cut(s) 408
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 3 cut(s) 903, 1068, 1505
TspGWI ACGGA 2 cut(s) 167, 465
TspRI CASTG 3 cut(s) 238, 1491, 1566
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 615
XagI CCTNNNNNAGG 1 cut(s) 836
XapI RAATTY 3 cut(s) 669, 723, 1107
XbaI TCTAGA 1 cut(s) 464
XceI RCATGY 1 cut(s) 719
XmnI GAANNNNTTC 2 cut(s) 1261, 1315
XspI CTAG 6 cut(s) 383, 465, 876, 980, 1320, 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.