Rroxscaffold_3G00268830

Plant protein of unknown function (DUF639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006760
Physical Location & Seq
Forward (+)
61878619 .. 61883214
4596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00268830.1

Sequence Viewer

Length: 2079 bp
ATGGCGGTCGCAAGCAAAACGAGAAATATGCTCGAGAATCTTGTAAGAGAAGGATCGTTCAAATGGTTGATTGGAAATCGAAGCCCTTTTGATGACGAATTGGAGGAGATGGAGAGGTCTCCGTCAGCCTCAACAAATTGGATACCGGAGCTATCTCCTATTGCAAATGTAGTTGTCCGTAGATGCTCCAAAATCCTTGGTGTTCCTACGACTGAGCTTTGTAAAGGCTTCAGTGCAGAGGCTTCCGAGTCTATAAAGCATCCATCTCGCTATGCAAGGAACTTTTTGGAATACTGCTCGTTCAGAGCTCTCGCTCTGTCAACTCAGGTGACAGGTCATCTGGCTGATAAAAAGTTTCGACGCCTAACATATGATATGATGCTAGCTTGGGAGGCTCCAGCTTCTGACAGCCAACCTTTGCTAAATTTAAATGAGGATTTATCAGTTGGGATAGAGGCTTTCTCTAGAATAGCTCCGTCAGTTCCCATTATTGCAAATGTGATTATCAGCGAAAATCTTTTTGAGGTACTTGCAACATCAACTGGTGGGAGACTTCAGTTCTCCACTTATGACAAGTATCTAAGTGGACTGGAAAGAGCAATAAGAAAAATGAGGACCCAATCAGAGTCATCTCTTCTTTCTGCTGTACGATCATCAAGAGGAGAGAGAATTCTGGAAGTGGATGGAACAGTAACTACACAACCAGTTCTTGAGCATGTGGGAATTTCTACATGGCCTGGACGGTTGATTCTGACAGATCATGCACTTTACTTTGAGGCTCTGCGTGTTGTATCTTATGACAAGGCAAAACGATATGACCTATCAGATGACCTAAAACAGGTCGTGAAACCTGAGTTGACCGGCCCATGGGGTACTAGACTGTTTGACAAGGCAGTCTTTTATAAATCAATTTCATTATCAGAACCAGTTGTGATAGAGTTTCCTGAGCTGAAGGGGCATTCTCGTCGTGATTACTGGCTAACGATCATAAGAGAGATTTTGTATGTTCACAGATTTATACATAAATACCAAATCAAGGGGGTCAAACGAGATGAAGCACTATCAAAGGCTGTGCTTGGGATTCTGCGAGTACAAGCCATTCAAGAAATTTCTTCTGCTCCTTTACACTCTGAGGGTCTTTTAATGTTCAATCTTTGTGATCAATTACCAGGTGGAGACTTGATACTGGAAACTCTTGCAAATATGTCAACCGTAAGCGAATTAAATAGGTCTGACACCTCTAAGTCTGGAGGTGGAATGTATTCGATCTCAGCGTTAGATATGATTTCTAACTTGGGCTTCGCATTTGGAACAAGTTCTAATAATTCGGTTGAGGCTGGTCTTGCTGTGGGTGAGGTAACTGTTGGACAACTGACTTCACTGGAAAGAGCAGTTAAGGAATCAAAAAACAATTATGAAAAAGTGGCACAAGCACAAGCAACAGTAGATGGAGTCAAAGTGGAAGGCATTGATACGAATGTTGCAGTGATGAAGGAGTTGCTCTTTCCAATGATGGAACTAGGGAAATGGCTTCTTTCTTTGGCATTATGGGAGGATCCACTGAAGTCTCTGGGTTTCTGTGTTGTTTTCACTTACATCATATGCAGGGGATGGCTGAGTTATGCCTTTGCATTTACGCTCGTCTTTATTGCGATCTTCATGATGCTCACCCGGCTTTTTAGCCAAGGAATATCCGATCACAAGGTTAAGGTGTTAGCACCACCACCAATGAACACAATGGAGCAGCTTTTGGCTGTTCAGAATGCAATTTCCCAAGCTGAAGGGATCGTCCAGGATGGGAACATTGTTCTCCTAAAGCTACGTGCTTTGTTGCTGTCACTTTTTCCGCAGGCAAGCGAAAAATTTGCAGTGGCTCTTTTAGTGATGGCTTCGACTCTGGCCTTCCTCCCCGGTAAGTACGTTGTTCTGTTGATCTTCTTGGAAGCGTTTACACGGTATTCACCAATGAGGAAAACTAGCACGGAGAGATGGATGAGAAGATTGCGAGAGTGGTGGTTCAGCGTACCGGCAGCTCCAGTTATACTTGAAAGAGAAAAGGAAGAGAAAAAGAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF639

Protein Analysis

692

Amino Acids

77.5

Weight (kDa)

8.48

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF639 PF04842 458 - 685 6.5e-85 Plant protein of unknown function (DUF639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AasI GACNNNNNNGTC 1 cut(s) 893
AciI CCGC 2 cut(s) 5, 1847
AclWI GGATC 4 cut(s) 61, 1550, 1563, 1793
AcsI RAATTY 5 cut(s) 424, 669, 723, 1107, 1862
AcuI CTGAAG 5 cut(s) 214, 539, 971, 1583, 1800
AcyI GRCGYC 1 cut(s) 361
AfaI GTAC 6 cut(s) 528, 648, 874, 1092, 1919, 2025
AfiI CCNNNNNNNGG 3 cut(s) 838, 867, 1036
AgsI TTSAA 4 cut(s) 61, 1103, 1150, 2048
AjnI CCWGG 3 cut(s) 736, 1168, 1791
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 4 cut(s) 123, 544, 1170, 1572
AlwI GGATC 4 cut(s) 61, 1550, 1563, 1793
AlwNI CAGNNNCTG 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 3 cut(s) 734, 862, 1899
ApeKI GCWGC 2 cut(s) 1744, 2030
ApoI RAATTY 5 cut(s) 424, 669, 723, 1107, 1862
Asp700I GAANNNNTTC 2 cut(s) 1261, 1315
AspS9I GGNCC 2 cut(s) 615, 863
AsuC2I CCSGG 2 cut(s) 1672, 1911
AsuHPI GGTGA 4 cut(s) 340, 1364, 1660, 1953
AsuNHI GCTAGC 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 1 cut(s) 615
BaeI ACNNNNGTAYC 2 cut(s) 1464, 1497
BamHI GGATCC 1 cut(s) 1555
BanII GRGCYC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 2 cut(s) 1756, 2042
BccI CCATC 9 cut(s) 103, 271, 677, 1442, 1507, 1605, 1790, 1879, 1983
BcgI CGANNNNNNTGC 8 cut(s) 10, 44, 248, 282, 947, 981, 1847, 1881
BciT130I CCWGG 3 cut(s) 738, 1170, 1793
BciVI GTATCC 1 cut(s) 135
BclI TGATCA 1 cut(s) 1159
BcnI CCSGG 2 cut(s) 1672, 1911
BcoDI GTCTC 4 cut(s) 123, 544, 1170, 1572
BfaI CTAG 5 cut(s) 383, 465, 876, 1520, 1977
BfuI GTATCC 1 cut(s) 135
BisI GCNGC 2 cut(s) 1745, 2031
BlsI GCNGC 2 cut(s) 1746, 2032
Bme1390I CCNGG 5 cut(s) 738, 1170, 1672, 1793, 1911
Bme18I GGWCC 1 cut(s) 615
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 2 cut(s) 615, 863
BmiI GGNNCC 3 cut(s) 396, 617, 1557
BmrFI CCNGG 5 cut(s) 738, 1170, 1672, 1793, 1911
BmsI GCATC 4 cut(s) 173, 268, 369, 1653
BmtI GCTAGC 1 cut(s) 386
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 3 cut(s) 381, 1269, 2019
Bpu10I CCTNAGC 1 cut(s) 945
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 2 cut(s) 1672, 1911
BsaAI YACGTR 1 cut(s) 1823
BsaHI GRCGYC 1 cut(s) 361
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 4 cut(s) 196, 866, 1684, 1909
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 2 cut(s) 1443, 1473
Bsc4I CCNNNNNNNGG 3 cut(s) 838, 867, 1036
Bse118I RCCGGY 2 cut(s) 860, 2026
Bse1I ACTGG 8 cut(s) 547, 594, 704, 926, 980, 1191, 1386, 2036
BseBI CCWGG 3 cut(s) 738, 1170, 1793
BseDI CCNNGG 4 cut(s) 196, 866, 1684, 1909
BseGI GGATG 5 cut(s) 259, 688, 1616, 1801, 1998
BseLI CCNNNNNNNGG 3 cut(s) 838, 867, 1036
BseMII CTCAG 7 cut(s) 204, 338, 843, 936, 1122, 1284, 1607
BseNI ACTGG 8 cut(s) 547, 594, 704, 926, 980, 1191, 1386, 2036
BseRI GAGGAG 2 cut(s) 119, 675
BseXI GCAGC 2 cut(s) 1756, 2042
BsgI GTGCAG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 3 cut(s) 736, 864, 1901
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 310
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 5 cut(s) 146, 861, 1672, 1911, 2027
BslI CCNNNNNNNGG 3 cut(s) 838, 867, 1036
BsmAI GTCTC 4 cut(s) 123, 544, 1170, 1572
BsmI GAATGC 2 cut(s) 958, 1768
BsnI GGCC 3 cut(s) 736, 864, 1901
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 2 cut(s) 5, 1847
BspANI GGCC 3 cut(s) 736, 864, 1901
BspCNI CTCAG 7 cut(s) 205, 337, 844, 937, 1123, 1283, 1608
BspHI TCATGA 1 cut(s) 1659
BspLI GGNNCC 3 cut(s) 396, 617, 1557
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 4 cut(s) 61, 1550, 1563, 1793
BspTNI GGTCTC 1 cut(s) 123
BsrFI RCCGGY 2 cut(s) 860, 2026
BsrI ACTGG 8 cut(s) 547, 594, 704, 926, 980, 1191, 1386, 2036
BssAI RCCGGY 2 cut(s) 860, 2026
BssECI CCNNGG 4 cut(s) 196, 866, 1684, 1909
BssNI GRCGYC 1 cut(s) 361
BssT1I CCWWGG 3 cut(s) 196, 866, 1684
Bst2UI CCWGG 3 cut(s) 738, 1170, 1793
Bst4CI ACNGT 7 cut(s) 691, 744, 882, 1213, 1363, 1444, 1956
Bst6I CTCTTC 3 cut(s) 639, 2055, 2066
BstACI GRCGYC 1 cut(s) 361
BstBAI YACGTR 1 cut(s) 1823
BstC8I GCNNGC 4 cut(s) 13, 384, 1851, 1855
BstDEI CTNAG 9 cut(s) 213, 324, 581, 852, 945, 1131, 1242, 1270, 1616
BstDSI CCRYGG 1 cut(s) 866
BstENI CCTNNNNNAGG 1 cut(s) 836
BstF5I GGATG 5 cut(s) 259, 688, 1616, 1801, 1998
BstMAI GTCTC 4 cut(s) 123, 544, 1170, 1572
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 392, 955, 1343, 1672
BstNI CCWGG 3 cut(s) 738, 1170, 1793
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 5 cut(s) 736, 1168, 1670, 1791, 1909
BstV1I GCAGC 2 cut(s) 1756, 2042
BstX2I RGATCY 1 cut(s) 1555
BstYI RGATCY 1 cut(s) 1555
BsuI GTATCC 1 cut(s) 135
BsuRI GGCC 3 cut(s) 736, 864, 1901
BtgI CCRYGG 1 cut(s) 866
BtsCI GGATG 5 cut(s) 259, 688, 1616, 1801, 1998
BtsI GCAGTG 2 cut(s) 1491, 1875
BtsIMutI CAGTG 5 cut(s) 238, 1379, 1491, 1559, 1875
Cac8I GCNNGC 4 cut(s) 13, 384, 1851, 1855
CaiI CAGNNNCTG 1 cut(s) 404
CciI TCATGA 1 cut(s) 1659
Cfr10I RCCGGY 2 cut(s) 860, 2026
Cfr13I GGNCC 2 cut(s) 615, 863
CseI GACGC 1 cut(s) 369
CsiI ACCWGGT 1 cut(s) 1168
Csp6I GTAC 6 cut(s) 527, 647, 873, 1091, 1918, 2024
CviAII CATG 5 cut(s) 716, 732, 761, 867, 1660
CviQI GTAC 6 cut(s) 527, 647, 873, 1091, 1918, 2024
DdeI CTNAG 9 cut(s) 213, 324, 581, 852, 945, 1131, 1242, 1270, 1616
DraI TTTAAA 1 cut(s) 429
DrdI GACNNNNNNGTC 1 cut(s) 893
DseDI GACNNNNNNGTC 1 cut(s) 893
Eam1104I CTCTTC 3 cut(s) 639, 2055, 2066
EarI CTCTTC 3 cut(s) 639, 2055, 2066
Ecl136II GAGCTC 1 cut(s) 308
Eco130I CCWWGG 3 cut(s) 196, 866, 1684
Eco24I GRGCYC 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 615
Eco53kI GAGCTC 1 cut(s) 308
Eco57I CTGAAG 5 cut(s) 214, 539, 971, 1583, 1800
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 308
EcoNI CCTNNNNNAGG 1 cut(s) 836
EcoO109I RGGNCCY 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 669
EcoRII CCWGG 3 cut(s) 736, 1168, 1791
EcoT14I CCWWGG 3 cut(s) 196, 866, 1684
EcoT38I GRGCYC 1 cut(s) 310
ErhI CCWWGG 3 cut(s) 196, 866, 1684
FaeI CATG 5 cut(s) 719, 735, 764, 870, 1663
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FatI CATG 5 cut(s) 715, 731, 760, 866, 1659
FauNDI CATATG 2 cut(s) 370, 1601
FbaI TGATCA 1 cut(s) 1159
Fnu4HI GCNGC 2 cut(s) 1745, 2031
FokI GGATG 5 cut(s) 246, 695, 1623, 1808, 2005
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 2 cut(s) 1745, 2031
FspBI CTAG 5 cut(s) 383, 465, 876, 1520, 1977
GluI GCNGC 2 cut(s) 1745, 2031
GsuI CTGGAG 3 cut(s) 381, 1269, 2019
HaeIII GGCC 3 cut(s) 736, 864, 1901
HapII CCGG 5 cut(s) 146, 861, 1672, 1911, 2027
HgaI GACGC 1 cut(s) 369
Hin1I GRCGYC 1 cut(s) 361
Hin1II CATG 5 cut(s) 719, 735, 764, 870, 1663
HincII GTYRAC 3 cut(s) 321, 858, 1209
HindII GTYRAC 3 cut(s) 321, 858, 1209
HinfI GANTC 8 cut(s) 37, 248, 626, 748, 1081, 1400, 1452, 1894
HpaII CCGG 5 cut(s) 146, 861, 1672, 1911, 2027
HphI GGTGA 4 cut(s) 340, 1364, 1660, 1953
Hpy166II GTNNAC 6 cut(s) 321, 587, 858, 1009, 1209, 1950
Hpy8I GTNNAC 6 cut(s) 321, 587, 858, 1009, 1209, 1950
Hpy99I CGWCG 2 cut(s) 363, 969
HpyAV CCTTC 6 cut(s) 44, 946, 1457, 1486, 1775, 1912
HpyCH4III ACNGT 7 cut(s) 691, 744, 882, 1213, 1363, 1444, 1956
HpyCH4IV ACGT 2 cut(s) 1822, 1920
HpyF10VI GCNNNNNNNGC 4 cut(s) 392, 955, 1343, 1672
HpyF3I CTNAG 9 cut(s) 213, 324, 581, 852, 945, 1131, 1242, 1270, 1616
HpySE526I ACGT 2 cut(s) 1822, 1920
Hsp92I GRCGYC 1 cut(s) 361
Hsp92II CATG 5 cut(s) 719, 735, 764, 870, 1663
Ksp22I TGATCA 1 cut(s) 1159
LmnI GCTCC 7 cut(s) 148, 191, 400, 478, 1123, 1741, 2038
Lsp1109I GCAGC 2 cut(s) 1756, 2042
LweI GCATC 4 cut(s) 173, 268, 369, 1653
MabI ACCWGGT 1 cut(s) 1168
MaeI CTAG 5 cut(s) 383, 465, 876, 1520, 1977
MaeII ACGT 2 cut(s) 1822, 1920
MaeIII GTNAC 4 cut(s) 328, 691, 1357, 1836
MboII GAAGA 6 cut(s) 626, 1104, 1648, 1927, 2010, 2072
MflI RGATCY 1 cut(s) 1555
MhlI GDGCHC 1 cut(s) 310
MlyI GAGTC 4 cut(s) 257, 635, 1461, 1888
MmeI TCCRAC 1 cut(s) 1345
MroXI GAANNNNTTC 2 cut(s) 1261, 1315
MseI TTAA 5 cut(s) 428, 1142, 1223, 1395, 1707
MspI CCGG 5 cut(s) 146, 861, 1672, 1911, 2027
MspR9I CCNGG 5 cut(s) 738, 1170, 1672, 1793, 1911
Mva1269I GAATGC 2 cut(s) 958, 1768
MvaI CCWGG 3 cut(s) 738, 1170, 1793
MwoI GCNNNNNNNGC 4 cut(s) 392, 955, 1343, 1672
NciI CCSGG 2 cut(s) 1672, 1911
NcoI CCATGG 1 cut(s) 866
NdeI CATATG 2 cut(s) 370, 1601
NheI GCTAGC 1 cut(s) 382
NlaIII CATG 5 cut(s) 719, 735, 764, 870, 1663
NlaIV GGNNCC 3 cut(s) 396, 617, 1557
NmuCI GTSAC 2 cut(s) 328, 1836
NspI RCATGY 1 cut(s) 719
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 1 cut(s) 1659
PcsI WCGNNNNNNNCGW 1 cut(s) 1271
PctI GAATGC 2 cut(s) 958, 1768
PdmI GAANNNNTTC 2 cut(s) 1261, 1315
PfeI GAWTC 4 cut(s) 37, 748, 1081, 1400
PfoI TCCNGGA 1 cut(s) 1791
PkrI GCNGC 2 cut(s) 1746, 2032
PleI GAGTC 4 cut(s) 256, 634, 1460, 1888
PpsI GAGTC 4 cut(s) 256, 634, 1460, 1888
Ppu21I YACGTR 1 cut(s) 1823
PpuMI RGGWCCY 1 cut(s) 615
PsiI TTATAA 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 615
Psp6I CCWGG 3 cut(s) 736, 1168, 1791
PspGI CCWGG 3 cut(s) 736, 1168, 1791
PspN4I GGNNCC 3 cut(s) 396, 617, 1557
PspPI GGNCC 2 cut(s) 615, 863
PspPPI RGGWCCY 1 cut(s) 615
PsrI GAACNNNNNNTAC 2 cut(s) 679, 711
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 1555
RsaI GTAC 6 cut(s) 528, 648, 874, 1092, 1919, 2025
RsaNI GTAC 6 cut(s) 527, 647, 873, 1091, 1918, 2024
SacI GAGCTC 1 cut(s) 310
SaqAI TTAA 5 cut(s) 428, 1142, 1223, 1395, 1707
SatI GCNGC 2 cut(s) 1745, 2031
Sau96I GGNCC 2 cut(s) 615, 863
SchI GAGTC 4 cut(s) 257, 635, 1461, 1888
ScrFI CCNGG 5 cut(s) 738, 1170, 1672, 1793, 1911
SduI GDGCHC 1 cut(s) 310
SexAI ACCWGGT 1 cut(s) 1168
SfaNI GCATC 4 cut(s) 173, 268, 369, 1653
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 1 cut(s) 615
SlaI CTCGAG 1 cut(s) 32
SmiI ATTTAAAT 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 32, 710
SmoI CTYRAG 2 cut(s) 32, 710
SsiI CCGC 2 cut(s) 5, 1847
SspMI CTAG 5 cut(s) 383, 465, 876, 1520, 1977
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 5 cut(s) 736, 1168, 1670, 1791, 1909
StyI CCWWGG 3 cut(s) 196, 866, 1684
SwaI ATTTAAAT 1 cut(s) 429
TaaI ACNGT 7 cut(s) 691, 744, 882, 1213, 1363, 1444, 1956
TaiI ACGT 2 cut(s) 1825, 1923
TaqI TCGA 5 cut(s) 33, 79, 358, 1265, 1892
TatI WGTACW 1 cut(s) 1090
TfiI GAWTC 4 cut(s) 37, 748, 1081, 1400
Tru1I TTAA 5 cut(s) 428, 1142, 1223, 1395, 1707
Tru9I TTAA 5 cut(s) 428, 1142, 1223, 1395, 1707
TscAI CASTG 5 cut(s) 238, 1386, 1491, 1566, 1875
TseFI GTSAC 2 cut(s) 328, 1836
TseI GCWGC 2 cut(s) 1744, 2030
Tsp45I GTSAC 2 cut(s) 328, 1836
TspDTI ATGAA 6 cut(s) 903, 1068, 1431, 1505, 1648, 1745
TspGWI ACGGA 4 cut(s) 111, 167, 465, 1997
TspRI CASTG 5 cut(s) 238, 1386, 1491, 1566, 1875
VpaK11BI GGWCC 1 cut(s) 615
XagI CCTNNNNNAGG 1 cut(s) 836
XapI RAATTY 5 cut(s) 424, 669, 723, 1107, 1862
XbaI TCTAGA 1 cut(s) 464
XceI RCATGY 1 cut(s) 719
XhoI CTCGAG 1 cut(s) 32
XmnI GAANNNNTTC 2 cut(s) 1261, 1315
XspI CTAG 5 cut(s) 383, 465, 876, 1520, 1977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.