MD14G1241800.v1.1

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0017668
Physical Location & Seq
Forward (+)
32051539 .. 32053662
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1241800.v1.1.491

Sequence Viewer

Length: 990 bp
ATGGAGGAGACTATAATTAATAGTAGCACAAAGAAATTAGTACCGGAAGTGGTGCTAAATTCAGGCCACAAGATGCCAGTGCTTGGGTTAGGGACAGGTAAAGTGCCTCCAACACCAACTGATGAGCTCGTCCCCATTCTCGTCGATGCCATTGAGGCCGGCTACCGCCATATCGACACTGCCGCTTTCTACCAAACCGAAGAAGCCGTCGGTCTAGCCGTGGTCCGAGCGAAAGAGCGAGGTCTTATCAAGAACCGGGATGACATGTTCATCACTTCCAAGCTGTGGTGTACTGATGCTTATCCTGATGGTGTTCTCCCAGCCCTCAAAACCTCACTCCAGAGGCTGGGGCTGGAGTACGTGGATCTGTACCTGATTCATTGGCCGTTGAGGCTGAAACATGGAGTCCCAGGATTTGAACTAACTAAAGAAGATATGATACCCTTTGATATAACGGGGACATGGGCGGCCATGGAAGAAATTTCCAGGTTAGGCTTAGCCAAGTCTATTGGCGTTAGCAATTTCGGCACCTTAAAGCTCTCTCAAATCCTTCACAATGCCACCATCCCCCCGGCGGTTAACCAGGTCGAAATGAACCCTTCGTGGCAGCAAGGAAAACTGCGAGACTTCTGTAAAGAGAAAGGAATTCATGTGACTGCGTGGTCTCCCTTGGGACTTTATGGAACTCCTCAGCTTAACAATGCTGGGATTGATTATTCCGTTCTCGAGGACCTCGCTGCTGCCAAAGGGAGGACTGTGCCACAGATTATACTGAGATCCATCGTCCAGCAAGGAGCAAGTGCAGTTCCGAGGACCTTCAACAAGGAGCGGATGAAGCCGAACCTTGACATATTCGATTGGGAACTAAATGAAGATGAAATGAACAAGATCAAGCAGATTCCCCAGCGCAGGCTGTGTTCCGCAGAGTTCTTTGTTTCCGAAACCGGACCTTGCAAGTCACTCGACCAACTCTGGGATAATGACCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

330

Amino Acids

36.65

Weight (kDa)

5.62

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 28 - 300 6.1e-49 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017668)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17900
malus_domestica MD14G1241800.v1.1
rosa_chinensis RchiOBHm_Chr7g0177711
rosa_laevigata RLG00000005512
rosa_multiflora Rmu_sc0004786.1_g000003
rosa_roxburghii Rroxscaffold_3G00275630
rosa_rugosa Rorug06G0408500
rosa_samantha Rh7AG008200 Rh7BG008200 Rh7CG008600 Rh7DG008300
rosa_wichuraiana Rw7G000690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 661
AccB1I GGYRCC 1 cut(s) 527
AccB7I CCANNNNNTGG 3 cut(s) 83, 285, 346
AccBSI CCGCTC 1 cut(s) 829
AciI CCGC 6 cut(s) 166, 183, 467, 575, 829, 921
AclWI GGATC 2 cut(s) 372, 771
AcoI YGGCCR 2 cut(s) 383, 468
AcsI RAATTY 3 cut(s) 58, 480, 645
AfaI GTAC 4 cut(s) 42, 292, 359, 371
AfiI CCNNNNNNNGG 7 cut(s) 83, 285, 346, 574, 750, 909, 973
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 2 cut(s) 419, 820
AjnI CCWGG 3 cut(s) 409, 485, 582
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 4 cut(s) 127, 283, 538, 694
AluI AGCT 4 cut(s) 127, 283, 538, 694
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 3 cut(s) 2, 618, 669
AlwI GGATC 2 cut(s) 372, 771
AlwNI CAGNNNCTG 1 cut(s) 346
Ama87I CYCGRG 1 cut(s) 725
AoxI GGCC 4 cut(s) 64, 156, 383, 468
ApeKI GCWGC 3 cut(s) 607, 737, 740
ApoI RAATTY 3 cut(s) 58, 480, 645
ArsI GACNNNNNNTTYG 2 cut(s) 192, 224
AseI ATTAAT 1 cut(s) 18
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 4 cut(s) 223, 730, 813, 947
AsuC2I CCSGG 2 cut(s) 257, 572
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 4 cut(s) 223, 730, 813, 947
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 1 cut(s) 129
BarI GAAGNNNNNNTAC 2 cut(s) 423, 455
Bbv12I GWGCWC 1 cut(s) 129
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 3 cut(s) 619, 724, 727
BccI CCATC 3 cut(s) 302, 572, 788
BceAI ACGGC 3 cut(s) 191, 203, 370
BcgI CGANNNNNNTGC 2 cut(s) 943, 977
BciT130I CCWGG 3 cut(s) 411, 487, 584
BcnI CCSGG 2 cut(s) 257, 572
BcoDI GTCTC 3 cut(s) 2, 618, 669
BfaI CTAG 1 cut(s) 215
BglI GCCNNNNNGGC 2 cut(s) 155, 391
BisI GCNGC 5 cut(s) 183, 468, 608, 738, 741
BlpI GCTNAGC 1 cut(s) 496
BlsI GCNGC 5 cut(s) 184, 469, 609, 739, 742
Bme1390I CCNGG 5 cut(s) 257, 411, 487, 572, 584
Bme18I GGWCC 4 cut(s) 223, 730, 813, 947
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 4 cut(s) 223, 730, 813, 947
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 5 cut(s) 257, 411, 487, 572, 584
BmsI GCATC 3 cut(s) 63, 136, 286
BpmI CTGGAG 2 cut(s) 323, 374
Bpu10I CCTNAGC 1 cut(s) 690
Bpu1102I GCTNAGC 1 cut(s) 496
BpuMI CCSGG 2 cut(s) 257, 572
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 669
BsaJI CCNNGG 6 cut(s) 219, 409, 471, 570, 669, 809
BsaWI WCCGGW 2 cut(s) 43, 944
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 285, 346, 574, 750, 909, 973
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 77
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 3 cut(s) 411, 487, 584
BseDI CCNNGG 6 cut(s) 219, 409, 471, 570, 669, 809
BseGI GGATG 3 cut(s) 265, 564, 837
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 7 cut(s) 83, 285, 346, 574, 750, 909, 973
BseMII CTCAG 2 cut(s) 704, 764
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 20, 678
BseXI GCAGC 3 cut(s) 619, 724, 727
BseYI CCCAGC 4 cut(s) 319, 346, 704, 903
BsgI GTGCAG 1 cut(s) 822
BshFI GGCC 4 cut(s) 66, 158, 385, 470
BshNI GGYRCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 5 cut(s) 44, 159, 256, 572, 945
BslFI GGGAC 5 cut(s) 106, 116, 392, 472, 687
BslI CCNNNNNNNGG 7 cut(s) 83, 285, 346, 574, 750, 909, 973
BsmAI GTCTC 3 cut(s) 2, 618, 669
BsmFI GGGAC 5 cut(s) 106, 116, 392, 472, 687
BsnI GGCC 4 cut(s) 66, 158, 385, 470
Bso31I GGTCTC 1 cut(s) 669
BsoBI CYCGRG 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 3 cut(s) 364, 776, 888
Bsp1720I GCTNAGC 1 cut(s) 496
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 6 cut(s) 166, 183, 467, 575, 829, 921
BspANI GGCC 4 cut(s) 66, 158, 385, 470
BspCNI CTCAG 2 cut(s) 703, 765
BspLI GGNNCC 1 cut(s) 529
BspPI GGATC 2 cut(s) 372, 771
BspT107I GGYRCC 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 669
BsrBI CCGCTC 1 cut(s) 829
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 6 cut(s) 219, 409, 471, 570, 669, 809
BssMI GATC 3 cut(s) 364, 776, 888
BssT1I CCWWGG 2 cut(s) 471, 669
Bst2UI CCWGG 3 cut(s) 411, 487, 584
Bst4CI ACNGT 1 cut(s) 757
BstBAI YACGTR 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 160, 911
BstDEI CTNAG 3 cut(s) 496, 690, 773
BstDSI CCRYGG 2 cut(s) 219, 471
BstF5I GGATG 3 cut(s) 265, 564, 837
BstHHI GCGC 1 cut(s) 909
BstKTI GATC 3 cut(s) 367, 779, 891
BstMAI GTCTC 3 cut(s) 2, 618, 669
BstMBI GATC 3 cut(s) 364, 776, 888
BstMWI GCNNNNNNNGC 4 cut(s) 155, 391, 525, 835
BstNI CCWGG 3 cut(s) 411, 487, 584
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 5 cut(s) 255, 409, 485, 570, 582
BstV1I GCAGC 3 cut(s) 619, 724, 727
BstX2I RGATCY 2 cut(s) 364, 776
BstYI RGATCY 2 cut(s) 364, 776
BsuRI GGCC 4 cut(s) 66, 158, 385, 470
BtgI CCRYGG 2 cut(s) 219, 471
BtsCI GGATG 3 cut(s) 265, 564, 837
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 2 cut(s) 84, 177
Cac8I GCNNGC 2 cut(s) 160, 911
CaiI CAGNNNCTG 1 cut(s) 346
CfoI GCGC 1 cut(s) 909
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 4 cut(s) 223, 730, 813, 947
CsiI ACCWGGT 1 cut(s) 582
Csp6I GTAC 4 cut(s) 41, 291, 358, 370
CviAII CATG 5 cut(s) 265, 401, 462, 472, 650
CviQI GTAC 4 cut(s) 41, 291, 358, 370
DdeI CTNAG 3 cut(s) 496, 690, 773
DpnI GATC 3 cut(s) 366, 778, 890
DpnII GATC 3 cut(s) 364, 776, 888
DrdI GACNNNNNNGTC 1 cut(s) 661
DseDI GACNNNNNNGTC 1 cut(s) 661
EaeI YGGCCR 2 cut(s) 383, 468
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 471, 669
Eco24I GRGCYC 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 669
Eco47I GGWCC 4 cut(s) 223, 730, 813, 947
Eco53kI GAGCTC 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 127
EcoO109I RGGNCCY 2 cut(s) 730, 813
EcoRI GAATTC 1 cut(s) 645
EcoRII CCWGG 3 cut(s) 409, 485, 582
EcoT14I CCWWGG 2 cut(s) 471, 669
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 471, 669
FaeI CATG 5 cut(s) 268, 404, 465, 475, 653
FaqI GGGAC 5 cut(s) 106, 116, 392, 472, 687
FatI CATG 5 cut(s) 264, 400, 461, 471, 649
Fnu4HI GCNGC 5 cut(s) 183, 468, 608, 738, 741
FokI GGATG 3 cut(s) 272, 551, 844
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 5 cut(s) 183, 468, 608, 738, 741
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 908
GluI GCNGC 5 cut(s) 183, 468, 608, 738, 741
GsaI CCCAGC 4 cut(s) 323, 350, 708, 907
GsuI CTGGAG 2 cut(s) 323, 374
HaeIII GGCC 4 cut(s) 66, 158, 385, 470
HapII CCGG 5 cut(s) 44, 159, 256, 572, 945
HhaI GCGC 1 cut(s) 909
Hin1II CATG 5 cut(s) 268, 404, 465, 475, 653
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 3 cut(s) 376, 405, 898
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 5 cut(s) 44, 159, 256, 572, 945
Hpy166II GTNNAC 2 cut(s) 291, 580
Hpy188I TCNGA 3 cut(s) 227, 810, 940
Hpy188III TCNNGA 4 cut(s) 250, 305, 340, 725
Hpy8I GTNNAC 2 cut(s) 291, 580
Hpy99I CGWCG 2 cut(s) 146, 212
HpyAV CCTTC 3 cut(s) 560, 609, 826
HpyCH4III ACNGT 1 cut(s) 757
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 2 cut(s) 803, 954
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 391, 525, 835
HpyF3I CTNAG 3 cut(s) 496, 690, 773
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 5 cut(s) 268, 404, 465, 475, 653
HspAI GCGC 1 cut(s) 907
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 3 cut(s) 364, 776, 888
LmnI GCTCC 2 cut(s) 794, 826
Lsp1109I GCAGC 3 cut(s) 619, 724, 727
LweI GCATC 3 cut(s) 63, 136, 286
MabI ACCWGGT 1 cut(s) 582
MaeI CTAG 1 cut(s) 215
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 652, 957
MalI GATC 3 cut(s) 366, 778, 890
MbiI CCGCTC 1 cut(s) 829
MboI GATC 3 cut(s) 364, 776, 888
MboII GAAGA 4 cut(s) 212, 443, 488, 884
MflI RGATCY 2 cut(s) 364, 776
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 6 cut(s) 15, 35, 58, 480, 520, 645
MlyI GAGTC 1 cut(s) 414
MmeI TCCRAC 1 cut(s) 134
MroNI GCCGGC 1 cut(s) 158
MseI TTAA 4 cut(s) 18, 533, 579, 696
MspI CCGG 5 cut(s) 44, 159, 256, 572, 945
MspR9I CCNGG 5 cut(s) 257, 411, 487, 572, 584
MvaI CCWGG 3 cut(s) 411, 487, 584
MwoI GCNNNNNNNGC 4 cut(s) 155, 391, 525, 835
NaeI GCCGGC 1 cut(s) 160
NciI CCSGG 2 cut(s) 257, 572
NcoI CCATGG 1 cut(s) 471
NdeII GATC 3 cut(s) 364, 776, 888
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 5 cut(s) 268, 404, 465, 475, 653
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 2 cut(s) 652, 957
NspI RCATGY 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 725
PciI ACATGT 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdiI GCCGGC 1 cut(s) 160
PfeI GAWTC 2 cut(s) 376, 898
PflMI CCANNNNNTGG 3 cut(s) 83, 285, 346
PkrI GCNGC 5 cut(s) 184, 469, 609, 739, 742
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Ppu21I YACGTR 1 cut(s) 361
PpuMI RGGWCCY 2 cut(s) 730, 813
PscI ACATGT 1 cut(s) 264
PshBI ATTAAT 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 129
Psp5II RGGWCCY 2 cut(s) 730, 813
Psp6I CCWGG 3 cut(s) 409, 485, 582
PspFI CCCAGC 4 cut(s) 319, 346, 704, 903
PspGI CCWGG 3 cut(s) 409, 485, 582
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 4 cut(s) 223, 730, 813, 947
PspPPI RGGWCCY 2 cut(s) 730, 813
PstNI CAGNNNCTG 1 cut(s) 346
PsuI RGATCY 2 cut(s) 364, 776
RsaI GTAC 4 cut(s) 42, 292, 359, 371
RsaNI GTAC 4 cut(s) 41, 291, 358, 370
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 4 cut(s) 18, 533, 579, 696
SatI GCNGC 5 cut(s) 183, 468, 608, 738, 741
Sau3AI GATC 3 cut(s) 364, 776, 888
Sau96I GGNCC 4 cut(s) 223, 730, 813, 947
SchI GAGTC 1 cut(s) 414
ScrFI CCNGG 5 cut(s) 257, 411, 487, 572, 584
SduI GDGCHC 1 cut(s) 129
SexAI ACCWGGT 1 cut(s) 582
SfaNI GCATC 3 cut(s) 63, 136, 286
Sfr274I CTCGAG 1 cut(s) 725
SinI GGWCC 4 cut(s) 223, 730, 813, 947
SlaI CTCGAG 1 cut(s) 725
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
Sse9I AATT 6 cut(s) 15, 35, 58, 480, 520, 645
SsiI CCGC 6 cut(s) 166, 183, 467, 575, 829, 921
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 5 cut(s) 255, 409, 485, 570, 582
StyI CCWWGG 2 cut(s) 471, 669
TaaI ACNGT 1 cut(s) 757
TaiI ACGT 1 cut(s) 363
TaqI TCGA 6 cut(s) 144, 174, 588, 726, 855, 963
TaqII GACCGA 1 cut(s) 200
TasI AATT 6 cut(s) 15, 35, 58, 480, 520, 645
TatI WGTACW 1 cut(s) 290
TauI GCSGC 2 cut(s) 185, 470
TfiI GAWTC 2 cut(s) 376, 898
Tru1I TTAA 4 cut(s) 18, 533, 579, 696
Tru9I TTAA 4 cut(s) 18, 533, 579, 696
TscAI CASTG 2 cut(s) 84, 184
TseFI GTSAC 2 cut(s) 652, 957
TseI GCWGC 3 cut(s) 607, 737, 740
Tsp45I GTSAC 2 cut(s) 652, 957
TspDTI ATGAA 8 cut(s) 259, 368, 608, 638, 848, 885, 891, 896
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 2 cut(s) 84, 184
Van91I CCANNNNNTGG 3 cut(s) 83, 285, 346
VpaK11BI GGWCC 4 cut(s) 223, 730, 813, 947
VspI ATTAAT 1 cut(s) 18
XapI RAATTY 3 cut(s) 58, 480, 645
XceI RCATGY 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 725
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.