MD14G1243700.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0017536
Physical Location & Seq
Reverse (-)
32218641 .. 32221015
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1243700.v1.1.491

Sequence Viewer

Length: 783 bp
ATGGCGCCAGTGGGATTACCTCCCGGTTTTAGGTTCCATCCAACGGACGAGGAGCTGGTGAATTATTATCTCAAGAGGAAAATCAACGGCCAAGAGATTGAGCTCGACATCATTCCTGAAGTTGATCTCTACAAATGCGAGCCTTGGGAATTAGCAGAAAAATCGTTTTTGCCAAGTAGAGACCCCGAGTGGTACTTCTTTGGACCCCGGGATCGAAAATACCCTAATGGATTCAGAACAAACAGAGCAACGAGAGCAGGATATTGGAAGTCGACTGGGAAAGACAGGAGAGTGACGTGCCAGAATCGAGCAATCGGAATGAAGAAGACGCTGGTTTACTACAGAGGCCGAGCCCCTCAGGGCATTCGGACTGACTGGGTCATGCACGAGTATCGCCTCGATGACAAGGACTACTACTGCTCCTCCGACCTCGACACCTCCTCCTCATCATCTGCCGCAATTCAGGATTCTTTTGCATTGTGTCGCGTGTTTAAGAAAAATGGCATTTGCGCGGAGATTGAAGAGCAGCAAGGCCAATCAAGTCATTTAACACTAAATAATATTCATGAACGATCACAAGGTGTAAACAATGATCATTGTGATCAAACCATGTCACCGGAGATTCTGATGGCATCGTCATCGTCGATCTCATGCCTGCAGGAGGAAGATGACAAAGACGATTCCTGGATGCAGTTCATCACAGATGATACGTGGATGCAGTGTTCTTCTAACACCGGTGTCAGTGGTGAAGATCAGCTCTCTCATGTGGCCTTCACAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

261

Amino Acids

29.92

Weight (kDa)

5.14

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 7 - 133 1.1e-49 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017536)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17730
fragaria_vesca FvH4_5g18130
malus_domestica MD14G1243700.v1.1
prunus_persica Prupe.5G241300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0177481
rosa_laevigata RLG00000005534
rosa_multiflora Rmu_sc0028619.1_g000003
rosa_roxburghii Rroxscaffold_3G00275840
rosa_rugosa Rorug06G0406400
rosa_samantha Rh7AG005900 Rh7BG005800
rosa_wichuraiana Rw7G000480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 272
AccII CGCG 2 cut(s) 486, 512
AciI CCGC 2 cut(s) 456, 512
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 88
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 581
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 30, 43, 661
AgeI ACCGGT 1 cut(s) 734
AgsI TTSAA 1 cut(s) 521
AjiI CACGTC 1 cut(s) 297
AjnI CCWGG 1 cut(s) 683
AloI GAACNNNNNNTCC 2 cut(s) 706, 738
AluBI AGCT 3 cut(s) 55, 103, 757
AluI AGCT 3 cut(s) 55, 103, 757
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 1 cut(s) 219
Ama87I CYCGRG 2 cut(s) 185, 207
AoxI GGCC 4 cut(s) 88, 346, 532, 768
ApeKI GCWGC 1 cut(s) 526
AsiGI ACCGGT 1 cut(s) 734
AspLEI GCGC 2 cut(s) 7, 512
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 3 cut(s) 24, 208, 209
AsuHPI GGTGA 3 cut(s) 70, 606, 758
AvaI CYCGRG 2 cut(s) 185, 207
AvaII GGWCC 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 357
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 105, 355
BauI CACGAG 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 332
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 538
BccI CCATC 2 cut(s) 45, 622
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 6 cut(s) 144, 178, 374, 408, 621, 655
BciT130I CCWGG 1 cut(s) 685
BclI TGATCA 2 cut(s) 592, 601
BcnI CCSGG 3 cut(s) 24, 208, 209
BcoDI GTCTC 1 cut(s) 174
BfmI CTRYAG 2 cut(s) 340, 656
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 2 cut(s) 456, 527
BlsI GCNGC 2 cut(s) 457, 528
Bme1390I CCNGG 4 cut(s) 24, 208, 209, 685
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 2 cut(s) 185, 207
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 3 cut(s) 6, 35, 205
BmrFI CCNGG 4 cut(s) 24, 208, 209, 685
BmrI ACTGGG 2 cut(s) 285, 385
BmsI GCATC 3 cut(s) 641, 678, 705
BmuI ACTGGG 2 cut(s) 285, 385
BpiI GAAGAC 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 3 cut(s) 24, 208, 209
BsaAI YACGTR 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 3 cut(s) 143, 206, 207
BsaWI WCCGGW 2 cut(s) 616, 734
BsaXI ACNNNNNCTCC 6 cut(s) 404, 407, 425, 434, 437, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 43, 661
Bse118I RCCGGY 1 cut(s) 734
Bse1I ACTGG 3 cut(s) 8, 280, 380
Bse21I CCTNAGG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 3 cut(s) 143, 206, 207
BseGI GGATG 3 cut(s) 37, 693, 720
BseLI CCNNNNNNNGG 3 cut(s) 30, 43, 661
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 3 cut(s) 8, 280, 380
BseRI GAGGAG 4 cut(s) 65, 412, 430, 433
BseXI GCAGC 1 cut(s) 538
Bsh1236I CGCG 2 cut(s) 486, 512
BshFI GGCC 4 cut(s) 90, 348, 534, 770
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 734
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 2 cut(s) 185, 207
BsiSI CCGG 4 cut(s) 24, 208, 617, 735
BslI CCNNNNNNNGG 3 cut(s) 30, 43, 661
BsmAI GTCTC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 4 cut(s) 90, 348, 534, 770
Bso31I GGTCTC 1 cut(s) 174
BsoBI CYCGRG 2 cut(s) 185, 207
Bsp1286I GDGCHC 2 cut(s) 105, 355
Bsp143I GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
BspACI CCGC 2 cut(s) 456, 512
BspANI GGCC 4 cut(s) 90, 348, 534, 770
BspCNI CTCAG 1 cut(s) 370
BspFNI CGCG 2 cut(s) 486, 512
BspHI TCATGA 1 cut(s) 565
BspLI GGNNCC 3 cut(s) 6, 35, 205
BspMAI CTGCAG 1 cut(s) 660
BspPI GGATC 1 cut(s) 219
BspQI GCTCTTC 1 cut(s) 516
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 734
BsrI ACTGG 3 cut(s) 8, 280, 380
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 3 cut(s) 143, 206, 207
BssMI GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 143
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 685
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 711
BstC8I GCNNGC 2 cut(s) 140, 656
BstDEI CTNAG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 659
BstF5I GGATG 3 cut(s) 37, 693, 720
BstFNI CGCG 2 cut(s) 486, 512
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 512
BstKTI GATC 7 cut(s) 127, 214, 575, 595, 604, 648, 754
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 685
BstSCI CCNGG 4 cut(s) 22, 206, 207, 683
BstSFI CTRYAG 2 cut(s) 340, 656
BstUI CGCG 2 cut(s) 486, 512
BstV1I GCAGC 1 cut(s) 538
BstV2I GAAGAC 1 cut(s) 332
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 4 cut(s) 90, 348, 534, 770
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 3 cut(s) 37, 693, 720
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 3 cut(s) 15, 725, 748
Cac8I GCNNGC 2 cut(s) 140, 656
CciI TCATGA 1 cut(s) 565
CfoI GCGC 2 cut(s) 7, 512
Cfr10I RCCGGY 1 cut(s) 734
Cfr13I GGNCC 1 cut(s) 203
Cfr9I CCCGGG 1 cut(s) 207
CseI GACGC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 193
CspAI ACCGGT 1 cut(s) 734
CviAII CATG 5 cut(s) 382, 566, 610, 651, 764
CviJI RGCY 9 cut(s) 55, 90, 103, 142, 348, 353, 534, 757, 770
CviKI_1 RGCY 9 cut(s) 55, 90, 103, 142, 348, 353, 534, 757, 770
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 357
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 126, 213, 574, 594, 603, 647, 753
DpnII GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
DraIII CACNNNGTG 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 2 cut(s) 105, 355
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 138
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 2 cut(s) 185, 207
EcoICRI GAGCTC 1 cut(s) 103
EcoNI CCTNNNNNAGG 1 cut(s) 659
EcoRII CCWGG 1 cut(s) 683
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 2 cut(s) 105, 355
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 5 cut(s) 385, 569, 613, 654, 767
FaiI YATR 5 cut(s) 383, 567, 611, 652, 765
FatI CATG 5 cut(s) 381, 565, 609, 650, 763
FbaI TGATCA 2 cut(s) 592, 601
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 2 cut(s) 456, 527
FokI GGATG 3 cut(s) 24, 700, 727
FriOI GRGCYC 2 cut(s) 105, 355
Fsp4HI GCNGC 2 cut(s) 456, 527
GlaI GCGC 2 cut(s) 6, 511
GluI GCNGC 2 cut(s) 456, 527
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 90, 348, 534, 770
HapII CCGG 4 cut(s) 24, 208, 617, 735
HgaI GACGC 1 cut(s) 337
HhaI GCGC 2 cut(s) 7, 512
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 385, 569, 613, 654, 767
Hin6I GCGC 2 cut(s) 5, 510
HinP1I GCGC 2 cut(s) 5, 510
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 5 cut(s) 231, 304, 467, 622, 680
HpaII CCGG 4 cut(s) 24, 208, 617, 735
HphI GGTGA 3 cut(s) 70, 606, 758
Hpy166II GTNNAC 3 cut(s) 273, 337, 586
Hpy188I TCNGA 5 cut(s) 236, 317, 369, 427, 627
Hpy188III TCNNGA 4 cut(s) 73, 116, 464, 566
Hpy8I GTNNAC 3 cut(s) 273, 337, 586
Hpy99I CGWCG 1 cut(s) 646
HpyAV CCTTC 1 cut(s) 781
HpyCH4IV ACGT 2 cut(s) 296, 710
HpyCH4V TGCA 5 cut(s) 385, 476, 658, 691, 718
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 2 cut(s) 296, 710
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 385, 569, 613, 654, 767
HspAI GCGC 2 cut(s) 5, 510
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 2 cut(s) 592, 601
Kzo9I GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 2 cut(s) 52, 425
Lsp1109I GCAGC 1 cut(s) 538
LweI GCATC 3 cut(s) 641, 678, 705
MaeII ACGT 2 cut(s) 296, 710
MaeIII GTNAC 2 cut(s) 292, 612
MalI GATC 7 cut(s) 126, 213, 574, 594, 603, 647, 753
MboI GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
MboII GAAGA 6 cut(s) 334, 337, 533, 677, 717, 761
MhlI GDGCHC 2 cut(s) 105, 355
MluCI AATT 3 cut(s) 61, 149, 459
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 65, 450
MseI TTAA 2 cut(s) 492, 548
MspI CCGG 4 cut(s) 24, 208, 617, 735
MspR9I CCNGG 4 cut(s) 24, 208, 209, 685
Mva1269I GAATGC 1 cut(s) 363
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 2 cut(s) 486, 512
MwoI GCNNNNNNNGC 1 cut(s) 254
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 3 cut(s) 24, 208, 209
NdeII GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
NlaIII CATG 5 cut(s) 385, 569, 613, 654, 767
NlaIV GGNNCC 3 cut(s) 6, 35, 205
NmeAIII GCCGAG 1 cut(s) 374
NmuCI GTSAC 2 cut(s) 292, 612
PagI TCATGA 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 641
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 5 cut(s) 231, 304, 467, 622, 680
PflFI GACNNNGTC 1 cut(s) 377
PfoI TCCNGGA 1 cut(s) 683
PinAI ACCGGT 1 cut(s) 734
PkrI GCNGC 2 cut(s) 457, 528
PluTI GGCGCC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 683
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 3 cut(s) 6, 35, 205
PspPI GGNCC 1 cut(s) 203
PstI CTGCAG 1 cut(s) 660
PsyI GACNNNGTC 1 cut(s) 377
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 2 cut(s) 492, 548
SatI GCNGC 2 cut(s) 456, 527
Sau3AI GATC 7 cut(s) 124, 211, 572, 592, 601, 645, 751
Sau96I GGNCC 1 cut(s) 203
SbfI CCTGCAGG 1 cut(s) 660
ScrFI CCNGG 4 cut(s) 24, 208, 209, 685
SdaI CCTGCAGG 1 cut(s) 660
SduI GDGCHC 2 cut(s) 105, 355
SfaNI GCATC 3 cut(s) 641, 678, 705
SfcI CTRYAG 2 cut(s) 340, 656
SfoI GGCGCC 1 cut(s) 6
SgrAI CRCCGGYG 1 cut(s) 734
SinI GGWCC 1 cut(s) 203
SmaI CCCGGG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse8387I CCTGCAGG 1 cut(s) 660
Sse9I AATT 3 cut(s) 61, 149, 459
SsiI CCGC 2 cut(s) 456, 512
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 562
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 4 cut(s) 22, 206, 207, 683
StyI CCWWGG 1 cut(s) 143
TaiI ACGT 2 cut(s) 299, 713
TaqI TCGA 7 cut(s) 105, 214, 272, 307, 399, 432, 644
TasI AATT 3 cut(s) 61, 149, 459
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 5 cut(s) 231, 304, 467, 622, 680
Tru1I TTAA 2 cut(s) 492, 548
Tru9I TTAA 2 cut(s) 492, 548
TscAI CASTG 3 cut(s) 15, 725, 748
TseFI GTSAC 2 cut(s) 292, 612
TseI GCWGC 1 cut(s) 526
Tsp45I GTSAC 2 cut(s) 292, 612
TspDTI ATGAA 4 cut(s) 335, 554, 582, 685
TspGWI ACGGA 1 cut(s) 59
TspMI CCCGGG 1 cut(s) 207
TspRI CASTG 3 cut(s) 15, 725, 748
Tth111I GACNNNGTC 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 659
XmaI CCCGGG 1 cut(s) 207
XmiI GTMKAC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.