Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008215

Physical Location & Seq

Genomic Coordinates Forward (+)

264147 .. 269737

Gene Structure 5591 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1004400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGCCGCTAAACGGAGCGGAAGGTGCCATCTGGGCACTGCTATGTCTCGCCGCTCTCTTCTCCATAGCCGTCGCTGAAGATCCCTACAGATTCTTCGAATGGAATGTCACTTACGGCGACATTTACCCTCTCGGTGTTCGCCAAAAGGGAATACTGATAAATGGACAGTTCCCGGGGCCAGACATCCACTCCGTCACCAATGACAATCTCATCATCAACGTCTTCAACAGCTTGGACGAGCCGTTCCTCCTCTCCTGGAACGGAATCCAACAAAGAAGGAATTCATTTGAGGACGGAGTGTATGGAACAACCTGCCCAATTCCACCGGGGAAGAACTTCACCTACATTCTCCAAGTCAAGGACCAAATCGGAAGTTTCTACTACTTCCCATCTCTCGCCTTCCACAAGGCTGCCGGCGGCTTCGGAGGCATCCGAATTCTTAGCAGGCCCAGAATTCCCGTCCCTTTCCCCGATCCTGCCGGTGATTACACTGTTCTTATTGGTGATTGGTACAAGGCTAATCACACGACCTTGAAGGCTCATCTGGACCGTGGTAAGAAGCTACCTTTCCCTGATGGAATCCTTATCAACGGCCGCGGTCCTGGTGGATTCTCTCTTAATTTTGAGCAAGGAAAAACATACAGGCTCAGAATTTCAAACATTGGGTTGCAAAATTCCCTCAACTTCCGCATTCAAAACCACAAAATGAAGCTGGTAGAAGTGGAGGGAACACACACCCTCCAAACCACCTACTCATCCCTTGATGTACACGTTGGCCAATCCTACTCGGTTCTTGTAACAGCTGATCAGCCTGGACAAGACTACTACCTTGTAGCTTCCTCTCGTTTCACCTCTCCAATCCTCACCACAACCGGCACTGTTCATTACGCCAACTCTGCTGGCAAAGTCTCTGGCCCACCCCCTGGTGGACCTACCATCCAAGTCGACTGGTCTTTGAACCAAGCCCGCTCTATCAGGACCAATCTTACAGCAAGTGGACCGAGGCCAAACCCGCAGGGCTCGTACCACTACGGACTGATCAATTTAACCAAAACTTATGTGCTACAAAATTCCGCTGGTCAAGTGAACGGTAAGCAAAGATATGGGGTGAACAGCGTGTCGTTTGTTCCAGCAGACACTCCCTTGAAGCTCGCGGACTACTTCAAAATTGGAGGAGTTTTCCGTGTTGGAAGCATCTCCGACAGGCCTACTGGTGGGGGATTGTACCTTGACACCTCAGTATTGGGTGCTGATTATAGAGCATTCGTCGAGATTGTGTTCCAAAACAACGAAGATATAATTCAGAGCTGGCATCTCGATGGTTACTCTTTCTTTGTTGTTGGTATGGATGGAGGGCAGTGGACAACTGCTAGCAGGAATGCGTATAATCTCAGAGATGCGGTTTCACGTTGCACCACTCAGGTGTATCCCAAGTCGTGGACGGCTATATACATTCCGCTAGATAATGTGGGAATGTGGAACTTGAGGACAGAGTTTTGGGCACGACAGTACCTTGGGCAACAGTTATATCTGCGTGTTTATACTCCATCCACTTCAATTAGGGATGAATACCCTATTCCAAGGAATGCGCGGCTATGTGGCAGAGCAAGCGGCCGGCGCACACGACCCCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

545

Amino Acids

60.42

Weight (kDa)

9.27

Isoelectric Point (pI)

31.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu-oxidase_3	PF07732	36 - 149	2.4e-37	Multicopper oxidase
Cu-oxidase	PF00394	162 - 298	3.1e-44	Multicopper oxidase
Cu-oxidase_2	PF07731	379 - 516	6.8e-32	Multicopper oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21850 AT1G21860 AT1G41830 AT1G76160
fragaria_vesca	FvH4_2g37780 FvH4_2g37780 FvH4_2g37780 FvH4_2g37780
malus_domestica	MD08G1005000.v1.1 MD15G1004400.v1.1
prunus_persica	Prupe.1G357100_v2.0.a1
pyrus_communis	pycom08g00390 pycom15g00330
rosa_chinensis	RchiOBHm_Chr6g0303931
rosa_laevigata	RLG00000011032
rosa_multiflora	Rmu_ssc0000205.1_g000001
rosa_roxburghii	Rroxscaffold_7G00164250
rosa_rugosa	Rorug06G0326100
rosa_samantha	Rh6AG440300 Rh6BG462700 Rh6CG452600 Rh6DG439600
rosa_wichuraiana	Rw6G038130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	320
AccB1I	GGYRCC	1 cut(s)	23
AccB7I	CCANNNNNTGG	2 cut(s)	923, 1437
AccBSI	CCGCTC	3 cut(s)	17, 53, 969
AccI	GTMKAC	1 cut(s)	945
AccII	CGCG	3 cut(s)	597, 1154, 1591
AclWI	GGATC	2 cut(s)	74, 467
AcoI	YGGCCR	3 cut(s)	592, 775, 1612
AcsI	RAATTY	6 cut(s)	280, 435, 453, 651, 673, 1069
AcuI	CTGAAG	1 cut(s)	96
AfaI	GTAC	5 cut(s)	512, 768, 1025, 1226, 1511
AfiI	CCNNNNNNNGG	6 cut(s)	11, 358, 923, 926, 1214, 1437
AflIII	ACRYGT	1 cut(s)	769
AgsI	TTSAA	8 cut(s)	226, 535, 657, 695, 958, 1147, 1165, 1557
AjnI	CCWGG	4 cut(s)	254, 601, 811, 922
AleI	CACNNNNGTG	1 cut(s)	1421
AloI	GAACNNNNNNTCC	2 cut(s)	227, 259
AluBI	AGCT	7 cut(s)	231, 562, 712, 803, 836, 1150, 1308
AluI	AGCT	7 cut(s)	231, 562, 712, 803, 836, 1150, 1308
Alw26I	GTCTC	2 cut(s)	50, 913
AlwI	GGATC	2 cut(s)	74, 467
Ama87I	CYCGRG	1 cut(s)	172
AoxI	GGCC	8 cut(s)	176, 446, 592, 775, 913, 1004, 1205, 1612
ApeKI	GCWGC	1 cut(s)	410
ApoI	RAATTY	6 cut(s)	280, 435, 453, 651, 673, 1069
ArsI	GACNNNNNNTTYG	2 cut(s)	90, 122
Asp700I	GAANNNNTTC	2 cut(s)	280, 335
AspLEI	GCGC	2 cut(s)	1591, 1620
AspS9I	GGNCC	9 cut(s)	176, 361, 447, 547, 599, 914, 929, 978, 998
AsuC2I	CCSGG	3 cut(s)	173, 174, 327
AsuHPI	GGTGA	7 cut(s)	187, 331, 494, 515, 841, 856, 1120
AsuII	TTCGAA	1 cut(s)	96
AsuNHI	GCTAGC	1 cut(s)	1370
AvaI	CYCGRG	1 cut(s)	172
AvaII	GGWCC	6 cut(s)	361, 547, 599, 929, 978, 998
BaeGI	GKGCMC	2 cut(s)	37, 1504
BaeI	ACNNNNGTAYC	2 cut(s)	1493, 1526
BalI	TGGCCA	1 cut(s)	777
BanI	GGYRCC	1 cut(s)	23
BanII	GRGCYC	1 cut(s)	1022
BbsI	GAAGAC	1 cut(s)	214
BbvI	GCAGC	1 cut(s)	397
BccI	CCATC	7 cut(s)	35, 397, 569, 944, 1313, 1343, 1555
BceAI	ACGGC	5 cut(s)	53, 130, 226, 607, 1458
BciT130I	CCWGG	4 cut(s)	256, 603, 813, 924
BciVI	GTATCC	1 cut(s)	1437
BclI	TGATCA	2 cut(s)	805, 1038
BcnI	CCSGG	3 cut(s)	173, 174, 327
BcoDI	GTCTC	2 cut(s)	50, 913
BfaI	CTAG	2 cut(s)	1371, 1460
BfmI	CTRYAG	1 cut(s)	85
BfuAI	ACCTGC	1 cut(s)	320
BfuI	GTATCC	1 cut(s)	1437
BglI	GCCNNNNNGGC	1 cut(s)	32
BisI	GCNGC	7 cut(s)	5, 51, 411, 418, 595, 1592, 1612
BlsI	GCNGC	7 cut(s)	6, 52, 412, 419, 596, 1593, 1613
Bme1390I	CCNGG	7 cut(s)	173, 174, 256, 327, 603, 813, 924
Bme18I	GGWCC	6 cut(s)	361, 547, 599, 929, 978, 998
BmeT110I	CYCGRG	1 cut(s)	172
BmgT120I	GGNCC	9 cut(s)	176, 361, 447, 547, 599, 914, 929, 978, 998
BmiI	GGNNCC	2 cut(s)	25, 177
BmrFI	CCNGG	7 cut(s)	173, 174, 256, 327, 603, 813, 924
BmsI	GCATC	4 cut(s)	438, 1203, 1321, 1387
BmtI	GCTAGC	1 cut(s)	1374
BpiI	GAAGAC	1 cut(s)	214
Bpu14I	TTCGAA	1 cut(s)	96
BpuEI	CTTGAG	1 cut(s)	1504
BpuMI	CCSGG	3 cut(s)	173, 174, 327
BsaJI	CCNNGG	9 cut(s)	172, 173, 326, 550, 595, 922, 1001, 1513, 1580
BsaXI	ACNNNNNCTCC	4 cut(s)	173, 203, 723, 753
Bsc4I	CCNNNNNNNGG	6 cut(s)	11, 358, 923, 926, 1214, 1437
Bse118I	RCCGGY	4 cut(s)	413, 479, 872, 1614
Bse1I	ACTGG	2 cut(s)	953, 1216
BseBI	CCWGG	4 cut(s)	256, 603, 813, 924
BseDI	CCNNGG	9 cut(s)	172, 173, 326, 550, 595, 922, 1001, 1513, 1580
BseGI	GGATG	7 cut(s)	183, 429, 755, 936, 1354, 1547, 1570
BseLI	CCNNNNNNNGG	6 cut(s)	11, 358, 923, 926, 1214, 1437
BseMII	CTCAG	4 cut(s)	661, 1251, 1405, 1433
BseNI	ACTGG	2 cut(s)	953, 1216
BseRI	GAGGAG	2 cut(s)	239, 1188
BseSI	GKGCMC	2 cut(s)	37, 1504
BseX3I	CGGCCG	2 cut(s)	592, 1612
BseXI	GCAGC	1 cut(s)	397
Bsh1236I	CGCG	3 cut(s)	597, 1154, 1591
Bsh1285I	CGRYCG	2 cut(s)	595, 1615
BshFI	GGCC	8 cut(s)	178, 448, 594, 777, 915, 1006, 1207, 1614
BshNI	GGYRCC	1 cut(s)	23
BsiEI	CGRYCG	2 cut(s)	595, 1615
BsiHKCI	CYCGRG	1 cut(s)	172
BsiSI	CCGG	6 cut(s)	173, 326, 414, 480, 873, 1615
BslFI	GGGAC	1 cut(s)	446
BslI	CCNNNNNNNGG	6 cut(s)	11, 358, 923, 926, 1214, 1437
BsmAI	GTCTC	2 cut(s)	50, 913
BsmFI	GGGAC	1 cut(s)	446
BsmI	GAATGC	4 cut(s)	690, 1262, 1384, 1591
BsnI	GGCC	8 cut(s)	178, 448, 594, 777, 915, 1006, 1207, 1614
BsoBI	CYCGRG	1 cut(s)	172
Bsp119I	TTCGAA	1 cut(s)	96
Bsp1286I	GDGCHC	3 cut(s)	37, 1022, 1504
Bsp1407I	TGTACA	1 cut(s)	766
Bsp143I	GATC	4 cut(s)	79, 472, 805, 1038
BspANI	GGCC	8 cut(s)	178, 448, 594, 777, 915, 1006, 1207, 1614
BspCNI	CTCAG	4 cut(s)	660, 1250, 1404, 1432
BspFNI	CGCG	3 cut(s)	597, 1154, 1591
BspLI	GGNNCC	2 cut(s)	25, 177
BspMI	ACCTGC	1 cut(s)	320
BspOI	GCTAGC	1 cut(s)	1374
BspPI	GGATC	2 cut(s)	74, 467
BspT104I	TTCGAA	1 cut(s)	96
BspT107I	GGYRCC	1 cut(s)	23
BsrBI	CCGCTC	3 cut(s)	17, 53, 969
BsrFI	RCCGGY	4 cut(s)	413, 479, 872, 1614
BsrGI	TGTACA	1 cut(s)	766
BsrI	ACTGG	2 cut(s)	953, 1216
BssAI	RCCGGY	4 cut(s)	413, 479, 872, 1614
BssECI	CCNNGG	9 cut(s)	172, 173, 326, 550, 595, 922, 1001, 1513, 1580
BssMI	GATC	4 cut(s)	79, 472, 805, 1038
BssT1I	CCWWGG	2 cut(s)	1513, 1580
Bst2UI	CCWGG	4 cut(s)	256, 603, 813, 924
Bst4CI	ACNGT	7 cut(s)	168, 493, 551, 880, 1091, 1509, 1524
Bst6I	CTCTTC	1 cut(s)	62
BstAUI	TGTACA	1 cut(s)	766
BstBI	TTCGAA	1 cut(s)	96
BstC8I	GCNNGC	9 cut(s)	415, 446, 901, 967, 1152, 1310, 1372, 1609, 1616
BstDEI	CTNAG	5 cut(s)	440, 647, 1237, 1391, 1419
BstDSI	CCRYGG	2 cut(s)	550, 595
BstF5I	GGATG	7 cut(s)	183, 429, 755, 936, 1354, 1547, 1570
BstFNI	CGCG	3 cut(s)	597, 1154, 1591
BstHHI	GCGC	2 cut(s)	1591, 1620
BstKTI	GATC	4 cut(s)	82, 475, 808, 1041
BstMAI	GTCTC	2 cut(s)	50, 913
BstMBI	GATC	4 cut(s)	79, 472, 805, 1038
BstMCI	CGRYCG	2 cut(s)	595, 1615
BstMWI	GCNNNNNNNGC	7 cut(s)	23, 32, 426, 896, 1012, 1608, 1617
BstNI	CCWGG	4 cut(s)	256, 603, 813, 924
BstSCI	CCNGG	7 cut(s)	171, 172, 254, 325, 601, 811, 922
BstSFI	CTRYAG	1 cut(s)	85
BstSLI	GKGCMC	2 cut(s)	37, 1504
BstUI	CGCG	3 cut(s)	597, 1154, 1591
BstV1I	GCAGC	1 cut(s)	397
BstV2I	GAAGAC	1 cut(s)	214
BstX2I	RGATCY	1 cut(s)	79
BstYI	RGATCY	1 cut(s)	79
BstZI	CGGCCG	2 cut(s)	592, 1612
BsuI	GTATCC	1 cut(s)	1437
BsuRI	GGCC	8 cut(s)	178, 448, 594, 777, 915, 1006, 1207, 1614
BtgI	CCRYGG	2 cut(s)	550, 595
BtsCI	GGATG	7 cut(s)	183, 429, 755, 936, 1354, 1547, 1570
BtsI	GCAGTG	2 cut(s)	35, 1364
BtsIMutI	CAGTG	4 cut(s)	35, 489, 876, 1364
BveI	ACCTGC	1 cut(s)	320
Cac8I	GCNNGC	9 cut(s)	415, 446, 901, 967, 1152, 1310, 1372, 1609, 1616
CfoI	GCGC	2 cut(s)	1591, 1620
Cfr10I	RCCGGY	4 cut(s)	413, 479, 872, 1614
Cfr13I	GGNCC	9 cut(s)	176, 361, 447, 547, 599, 914, 929, 978, 998
Cfr42I	CCGCGG	1 cut(s)	598
Cfr9I	CCCGGG	1 cut(s)	172
Csp6I	GTAC	5 cut(s)	511, 767, 1024, 1225, 1510
CviQI	GTAC	5 cut(s)	511, 767, 1024, 1225, 1510
DdeI	CTNAG	5 cut(s)	440, 647, 1237, 1391, 1419
DpnI	GATC	4 cut(s)	81, 474, 807, 1040
DpnII	GATC	4 cut(s)	79, 472, 805, 1038
EaeI	YGGCCR	3 cut(s)	592, 775, 1612
EagI	CGGCCG	2 cut(s)	592, 1612
Eam1104I	CTCTTC	1 cut(s)	62
EarI	CTCTTC	1 cut(s)	62
EclXI	CGGCCG	2 cut(s)	592, 1612
Eco130I	CCWWGG	2 cut(s)	1513, 1580
Eco147I	AGGCCT	1 cut(s)	1207
Eco24I	GRGCYC	1 cut(s)	1022
Eco47I	GGWCC	6 cut(s)	361, 547, 599, 929, 978, 998
Eco52I	CGGCCG	2 cut(s)	592, 1612
Eco57I	CTGAAG	1 cut(s)	96
Eco88I	CYCGRG	1 cut(s)	172
EcoRI	GAATTC	3 cut(s)	280, 435, 453
EcoRII	CCWGG	4 cut(s)	254, 601, 811, 922
EcoT14I	CCWWGG	2 cut(s)	1513, 1580
EcoT38I	GRGCYC	1 cut(s)	1022
ErhI	CCWWGG	2 cut(s)	1513, 1580
FaqI	GGGAC	1 cut(s)	446
FauI	CCCGC	2 cut(s)	974, 1020
FbaI	TGATCA	2 cut(s)	805, 1038
FblI	GTMKAC	1 cut(s)	945
Fnu4HI	GCNGC	7 cut(s)	5, 51, 411, 418, 595, 1592, 1612
FokI	GGATG	7 cut(s)	170, 416, 742, 923, 1361, 1534, 1577
FriOI	GRGCYC	1 cut(s)	1022
Fsp4HI	GCNGC	7 cut(s)	5, 51, 411, 418, 595, 1592, 1612
FspBI	CTAG	2 cut(s)	1371, 1460
GlaI	GCGC	2 cut(s)	1590, 1619
GluI	GCNGC	7 cut(s)	5, 51, 411, 418, 595, 1592, 1612
HaeIII	GGCC	8 cut(s)	178, 448, 594, 777, 915, 1006, 1207, 1614
HapII	CCGG	6 cut(s)	173, 326, 414, 480, 873, 1615
HhaI	GCGC	2 cut(s)	1591, 1620
Hin6I	GCGC	2 cut(s)	1589, 1618
HinP1I	GCGC	2 cut(s)	1589, 1618
HincII	GTYRAC	1 cut(s)	946
HindII	GTYRAC	1 cut(s)	946
HinfI	GANTC	4 cut(s)	90, 264, 579, 609
HpaII	CCGG	6 cut(s)	173, 326, 414, 480, 873, 1615
HphI	GGTGA	7 cut(s)	187, 331, 494, 515, 841, 856, 1120
Hpy166II	GTNNAC	8 cut(s)	769, 929, 946, 998, 1087, 1111, 1362, 1440
Hpy188I	TCNGA	7 cut(s)	371, 425, 434, 650, 1201, 1305, 1394
Hpy188III	TCNNGA	4 cut(s)	545, 976, 1270, 1316
Hpy8I	GTNNAC	8 cut(s)	769, 929, 946, 998, 1087, 1111, 1362, 1440
Hpy99I	CGWCG	2 cut(s)	74, 1271
HpyAV	CCTTC	4 cut(s)	14, 270, 409, 529
HpyCH4III	ACNGT	7 cut(s)	168, 493, 551, 880, 1091, 1509, 1524
HpyCH4IV	ACGT	3 cut(s)	219, 771, 1408
HpyCH4V	TGCA	2 cut(s)	670, 1413
HpyF10VI	GCNNNNNNNGC	7 cut(s)	23, 32, 426, 896, 1012, 1608, 1617
HpyF3I	CTNAG	5 cut(s)	440, 647, 1237, 1391, 1419
HpySE526I	ACGT	3 cut(s)	219, 771, 1408
HspAI	GCGC	2 cut(s)	1589, 1618
KroI	GCCGGC	2 cut(s)	413, 1614
KroNI	GCCGGC	2 cut(s)	415, 1616
Ksp22I	TGATCA	2 cut(s)	805, 1038
KspI	CCGCGG	1 cut(s)	598
Kzo9I	GATC	4 cut(s)	79, 472, 805, 1038
LmnI	GCTCC	1 cut(s)	14
Lsp1109I	GCAGC	1 cut(s)	397
LweI	GCATC	4 cut(s)	438, 1203, 1321, 1387
MaeI	CTAG	2 cut(s)	1371, 1460
MaeII	ACGT	3 cut(s)	219, 771, 1408
MaeIII	GTNAC	4 cut(s)	106, 193, 796, 1322
MalI	GATC	4 cut(s)	81, 474, 807, 1040
MbiI	CCGCTC	3 cut(s)	17, 53, 969
MboI	GATC	4 cut(s)	79, 472, 805, 1038
MboII	GAAGA	6 cut(s)	49, 85, 89, 214, 343, 1304
MflI	RGATCY	1 cut(s)	79
MhlI	GDGCHC	3 cut(s)	37, 1022, 1504
MlsI	TGGCCA	1 cut(s)	777
MluNI	TGGCCA	1 cut(s)	777
MmeI	TCCRAC	3 cut(s)	292, 1168, 1224
Mox20I	TGGCCA	1 cut(s)	777
MroNI	GCCGGC	2 cut(s)	413, 1614
MroXI	GAANNNNTTC	2 cut(s)	280, 335
MscI	TGGCCA	1 cut(s)	777
MseI	TTAA	2 cut(s)	618, 1046
MslI	CAYNNNNRTG	3 cut(s)	40, 1317, 1421
Msp20I	TGGCCA	1 cut(s)	777
MspA1I	CMGCKG	3 cut(s)	597, 803, 1076
MspI	CCGG	6 cut(s)	173, 326, 414, 480, 873, 1615
MspR9I	CCNGG	7 cut(s)	173, 174, 256, 327, 603, 813, 924
Mva1269I	GAATGC	4 cut(s)	690, 1262, 1384, 1591
MvaI	CCWGG	4 cut(s)	256, 603, 813, 924
MvnI	CGCG	3 cut(s)	597, 1154, 1591
MwoI	GCNNNNNNNGC	7 cut(s)	23, 32, 426, 896, 1012, 1608, 1617
NaeI	GCCGGC	2 cut(s)	415, 1616
NciI	CCSGG	3 cut(s)	173, 174, 327
NdeII	GATC	4 cut(s)	79, 472, 805, 1038
NgoMIV	GCCGGC	2 cut(s)	413, 1614
NheI	GCTAGC	1 cut(s)	1370
NlaIV	GGNNCC	2 cut(s)	25, 177
NmuCI	GTSAC	2 cut(s)	106, 193
NspV	TTCGAA	1 cut(s)	96
OliI	CACNNNNGTG	1 cut(s)	1421
PceI	AGGCCT	1 cut(s)	1207
PctI	GAATGC	4 cut(s)	690, 1262, 1384, 1591
PdiI	GCCGGC	2 cut(s)	415, 1616
PdmI	GAANNNNTTC	2 cut(s)	280, 335
PfeI	GAWTC	4 cut(s)	90, 264, 579, 609
PflMI	CCANNNNNTGG	2 cut(s)	923, 1437
PfoI	TCCNGGA	1 cut(s)	254
PkrI	GCNGC	7 cut(s)	6, 52, 412, 419, 596, 1593, 1613
Psp6I	CCWGG	4 cut(s)	254, 601, 811, 922
PspGI	CCWGG	4 cut(s)	254, 601, 811, 922
PspN4I	GGNNCC	2 cut(s)	25, 177
PspPI	GGNCC	9 cut(s)	176, 361, 447, 547, 599, 914, 929, 978, 998
PsrI	GAACNNNNNNTAC	2 cut(s)	326, 358
PsuI	RGATCY	1 cut(s)	79
PvuII	CAGCTG	1 cut(s)	803
RsaI	GTAC	5 cut(s)	512, 768, 1025, 1226, 1511
RsaNI	GTAC	5 cut(s)	511, 767, 1024, 1225, 1510
RseI	CAYNNNNRTG	3 cut(s)	40, 1317, 1421
SacII	CCGCGG	1 cut(s)	598
SalI	GTCGAC	1 cut(s)	944
SaqAI	TTAA	2 cut(s)	618, 1046
SatI	GCNGC	7 cut(s)	5, 51, 411, 418, 595, 1592, 1612
Sau3AI	GATC	4 cut(s)	79, 472, 805, 1038
Sau96I	GGNCC	9 cut(s)	176, 361, 447, 547, 599, 914, 929, 978, 998
ScrFI	CCNGG	7 cut(s)	173, 174, 256, 327, 603, 813, 924
SduI	GDGCHC	3 cut(s)	37, 1022, 1504
SfaNI	GCATC	4 cut(s)	438, 1203, 1321, 1387
SfcI	CTRYAG	1 cut(s)	85
Sfr303I	CCGCGG	1 cut(s)	598
SfuI	TTCGAA	1 cut(s)	96
SgrBI	CCGCGG	1 cut(s)	598
SinI	GGWCC	6 cut(s)	361, 547, 599, 929, 978, 998
SmaI	CCCGGG	1 cut(s)	174
SmiMI	CAYNNNNRTG	3 cut(s)	40, 1317, 1421
SmlI	CTYRAG	1 cut(s)	1483
SmoI	CTYRAG	1 cut(s)	1483
SseBI	AGGCCT	1 cut(s)	1207
SspMI	CTAG	2 cut(s)	1371, 1460
StuI	AGGCCT	1 cut(s)	1207
StyD4I	CCNGG	7 cut(s)	171, 172, 254, 325, 601, 811, 922
StyI	CCWWGG	2 cut(s)	1513, 1580
TaaI	ACNGT	7 cut(s)	168, 493, 551, 880, 1091, 1509, 1524
TaiI	ACGT	3 cut(s)	222, 774, 1411
TaqI	TCGA	4 cut(s)	96, 945, 1269, 1317
TaqII	GACCGA	1 cut(s)	1015
TatI	WGTACW	1 cut(s)	766
TauI	GCSGC	6 cut(s)	7, 53, 420, 597, 1594, 1614
TfiI	GAWTC	4 cut(s)	90, 264, 579, 609
Tru1I	TTAA	2 cut(s)	618, 1046
Tru9I	TTAA	2 cut(s)	618, 1046
TscAI	CASTG	4 cut(s)	42, 496, 883, 1364
TseFI	GTSAC	2 cut(s)	106, 193
TseI	GCWGC	1 cut(s)	410
Tsp45I	GTSAC	2 cut(s)	106, 193
TspDTI	ATGAA	4 cut(s)	273, 722, 872, 1581
TspGWI	ACGGA	6 cut(s)	27, 181, 276, 309, 1047, 1172
TspMI	CCCGGG	1 cut(s)	172
TspRI	CASTG	4 cut(s)	42, 496, 883, 1364
Van91I	CCANNNNNTGG	2 cut(s)	923, 1437
VpaK11BI	GGWCC	6 cut(s)	361, 547, 599, 929, 978, 998
XapI	RAATTY	6 cut(s)	280, 435, 453, 651, 673, 1069
XmaI	CCCGGG	1 cut(s)	172
XmiI	GTMKAC	1 cut(s)	945
XmnI	GAANNNNTTC	2 cut(s)	280, 335
XspI	CTAG	2 cut(s)	1371, 1460

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.