Rorug06G0326100

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008215
Physical Location & Seq
Forward (+)
47796263 .. 47797632
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0326100.1

Sequence Viewer

Length: 972 bp
ATGGCGAGCAGTGGCAGTGGGCAACAACTGCCGCCGTACAACCCTTACCGGCACGTGAAGACCCTTCGCGGCCACGAGAGCGCCGTGTCGTGCGTCAAATTCTCCAACGACGGAACCCTCTTGGCCTCGGCGTCGTTAGACAAGACCGTGATCCTCTGGTCGTCTTCCACCCTAGCCCTCCTGCGCCGCCTGGTGGGACACTCCGAGGGCATCTCCGACCTTTCCTGGTCCTCCGACTCCCACTACATCTGCTCCGCCTCCGACGACCGCACCCTCCGCATCTGGGACGCACTCTCCCCCACCGGCGAGTGCGTCAAGACCCTCCGCGGCCACTCCGACCTCGTTTTCTGCGCCAACTTCAACCCCCAGTCCAACCTCATCGTCTCCGGCTCGTTCGACGAGACGATTCGGATTTGGGAGGTCAAGACCGGCAAGTGCCTCCACGTCATCAGGGCCCACTCGCTTCCGGTGACCTCCGTGAGCTTCAATCGCGATGGGTCGCTCATCGTCTCCGCCAGTCACGACGGGTCGTGCAAGATTTGGGACACCGCTAAAGGGACCTGCTTGAAAACCCTAATTGACGATAAGGTCCCTGCCGTTTCCTCCGCCAAGTTCTCCCCCAACGGAAAATTCATACTCATCGCCACTCTAGATAATTCCCTTAAGCTGTGCAACTACTCGACGGGGAAGTTCTTGAAGGTCTATACGGGGCATTTGAACAAAGTGTATTGCATAACATCTACATTTTCTGTGACAAATGGGAAATATATTGTTAGTGGTTCCGAGGATCACTGTGTATACTTGTGGGATCTTCAGTCGAAAACTATGATTCAGAGATTTGAAGGCCATACTGATACCGTCATCTCTGTTTCCTGTCACCCGTCGGAGAATAAGATTGCCTCCGCAGCTCTTCAAAATGATAAGACTGTCCGGATTTGGGTTCAAGATAATGCCAATTCAGCTCCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

323

Amino Acids

35.31

Weight (kDa)

8.57

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 15 - 181 6.7e-16 MABP1/WDR62 second WD40 domain
WDR55 PF24796 17 - 145 2.6e-14 WDR55
Beta-prop_CAF1B_HIR1 PF24105 17 - 97 1.3e-10 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 17 - 313 1.2e-27 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 17 - 98 8.3e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 17 - 97 1.6e-18 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 17 - 140 2e-21 WDR3 second beta-propeller domain
WD40 PF00400 18 - 54 1.3e-11 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 18 - 97 1.7e-11 TEP-1 second beta-propeller
WD40_Prp19 PF24814 18 - 98 8.4e-12 Prp19 WD40 domain
Beta-prop_IFT122_1st PF23381 19 - 88 1.8e-07 IFT122 first beta-propeller
Beta-prop_WDR3_1st PF25173 20 - 96 1.9e-19 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 21 - 313 6.2e-133 WDR5 beta-propeller domain
EIF3I PF24805 21 - 107 2.3e-11 EIF3I
Beta-prop_EML PF23409 23 - 129 2.9e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin PF25460 25 - 110 8.6e-08 Aladin seven-bladed propeller
Beta-prop_WDR36-Utp21_2nd PF25168 59 - 98 1.7e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 60 - 96 1.8e-10 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 61 - 154 1.6e-13 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 67 - 280 3.1e-44 THOC3 beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 69 - 173 8.5e-08 MABP1/WDR62 first WD40 domain
WDR90_beta-prop_4th PF23342 72 - 201 1.9e-08 WDR90, 4th beta-propeller
WD40_WDHD1_1st PF24817 73 - 150 5.4e-12 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 77 - 141 1.9e-07 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 83 - 187 9.1e-10 WDR90/POC16, second beta-propeller
Beta-prop_SCAP PF24017 84 - 189 2.4e-07 SCAP Beta-propeller
EIF3I PF24805 100 - 152 3e-09 EIF3I
Beta-prop_WDR3_1st PF25173 101 - 195 1e-22 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 101 - 272 1.2e-32 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 101 - 223 9.1e-12 WDR36/Utp21 first beta-propeller
Beta-prop_Aladin PF25460 101 - 220 2.2e-11 Aladin seven-bladed propeller
WD40 PF00400 102 - 139 1.7e-10 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 102 - 162 5.1e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 104 - 196 3.7e-11 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 104 - 204 5.4e-14 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 110 - 214 2.3e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2 PF23414 120 - 275 6.4e-19 Echinoderm microtubule-associated protein second beta-propeller
eIF2A PF08662 123 - 225 9.3e-06 Eukaryotic translation initiation factor eIF2A
WDR55 PF24796 124 - 183 2.7e-08 WDR55
Beta-prop_SCAP PF24017 127 - 278 3.3e-08 SCAP Beta-propeller
WD40 PF00400 144 - 182 7.9e-12 WD domain, G-beta repeat
NBCH_WD40 PF20426 145 - 301 1.2e-09 Neurobeachin beta propeller domain
Beta-prop_WDR3_2nd PF25172 147 - 193 3e-06 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 149 - 280 9.9e-18 WDHD1 first WD40 domain
EIF3I PF24805 150 - 234 2.1e-08 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 151 - 315 8.1e-14 WDR36/Utp21 second beta-propeller domain
Beta-prop_ACSF4 PF13570 161 - 305 2.5e-08 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_TEP1_2nd PF25047 196 - 314 1.2e-09 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 198 - 314 1.4e-17 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 199 - 318 1.6e-15 CDC20/Fizzy WD40 domain
Beta-prop_IFT122_1st PF23381 199 - 303 3.4e-07 IFT122 first beta-propeller
WDR55 PF24796 199 - 302 1.3e-10 WDR55
Beta-prop_WDR3_2nd PF25172 200 - 317 5.1e-12 WDR3 second beta-propeller domain
EIF3I PF24805 200 - 304 2.4e-06 EIF3I
WD40_MABP1-WDR62_2nd PF24782 204 - 271 3.6e-06 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 209 - 319 3e-12 WDHD1 first WD40 domain
Beta-prop_EML PF23409 229 - 299 1.7e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 229 - 270 2.2e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 232 - 316 8.9e-08 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 248 - 314 5e-07 MABP1/WDR62 first WD40 domain
WD40_Prp19 PF24814 250 - 319 3.8e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 253 - 316 1.6e-12 THOC3 beta-propeller domain
WD40 PF00400 276 - 313 3.3e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 587
Acc36I ACCTGC 1 cut(s) 569
AccI GTMKAC 1 cut(s) 798
AccII CGCG 3 cut(s) 69, 327, 492
AccIII TCCGGA 1 cut(s) 930
AclWI GGATC 3 cut(s) 145, 795, 816
AcoI YGGCCR 2 cut(s) 70, 328
AcsI RAATTY 2 cut(s) 98, 629
AcuI CTGAAG 1 cut(s) 797
AcvI CACGTG 1 cut(s) 55
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 4 cut(s) 193, 283, 555, 937
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 8 cut(s) 361, 487, 568, 697, 718, 842, 914, 944
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 2 cut(s) 189, 224
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AluBI AGCT 4 cut(s) 483, 667, 908, 962
AluI AGCT 4 cut(s) 483, 667, 908, 962
Alw26I GTCTC 3 cut(s) 388, 395, 514
AlwI GGATC 3 cut(s) 145, 795, 816
Aor13HI TCCGGA 1 cut(s) 930
AoxI GGCC 5 cut(s) 70, 123, 328, 453, 844
ApaI GGGCCC 1 cut(s) 457
ApeKI GCWGC 1 cut(s) 905
ApoI RAATTY 2 cut(s) 98, 629
AspLEI GCGC 3 cut(s) 83, 186, 353
AspS9I GGNCC 5 cut(s) 228, 453, 454, 558, 589
AsuHPI GGTGA 2 cut(s) 481, 869
AvaII GGWCC 3 cut(s) 228, 558, 589
BaeGI GKGCMC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 457
BauI CACGAG 1 cut(s) 74
BbrPI CACGTG 1 cut(s) 55
BbsI GAAGAC 2 cut(s) 65, 156
BbvI GCAGC 1 cut(s) 917
BccI CCATC 1 cut(s) 488
BceAI ACGGC 3 cut(s) 19, 68, 581
BciT130I CCWGG 2 cut(s) 191, 226
BcoDI GTCTC 3 cut(s) 388, 395, 514
BfaI CTAG 2 cut(s) 173, 650
BfoI RGCGCY 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 662
BfuAI ACCTGC 1 cut(s) 569
BisI GCNGC 5 cut(s) 32, 70, 187, 328, 906
BlsI GCNGC 5 cut(s) 33, 71, 188, 329, 907
Bme1390I CCNGG 2 cut(s) 191, 226
Bme18I GGWCC 3 cut(s) 228, 558, 589
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 5 cut(s) 228, 453, 454, 558, 589
BmiI GGNNCC 5 cut(s) 115, 455, 559, 591, 781
BmrFI CCNGG 2 cut(s) 191, 226
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 2 cut(s) 219, 288
BmuI ACTGGG 1 cut(s) 361
BpiI GAAGAC 2 cut(s) 65, 156
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 4 cut(s) 126, 204, 325, 783
BsaWI WCCGGW 2 cut(s) 466, 930
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 278, 308
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 283, 555, 937
Bse118I RCCGGY 3 cut(s) 48, 302, 428
Bse1I ACTGG 2 cut(s) 367, 516
BseAI TCCGGA 1 cut(s) 930
BseBI CCWGG 2 cut(s) 191, 226
BseDI CCNNGG 4 cut(s) 126, 204, 325, 783
BseLI CCNNNNNNNGG 4 cut(s) 193, 283, 555, 937
BseNI ACTGG 2 cut(s) 367, 516
BseSI GKGCMC 1 cut(s) 457
BseXI GCAGC 1 cut(s) 917
Bsh1236I CGCG 3 cut(s) 69, 327, 492
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 5 cut(s) 72, 125, 330, 455, 846
BsiEI CGRYCG 1 cut(s) 268
BsiSI CCGG 6 cut(s) 49, 303, 387, 429, 467, 931
BslFI GGGAC 5 cut(s) 210, 299, 557, 571, 575
BslI CCNNNNNNNGG 4 cut(s) 193, 283, 555, 937
BsmAI GTCTC 3 cut(s) 388, 395, 514
BsmBI CGTCTC 3 cut(s) 388, 395, 514
BsmFI GGGAC 5 cut(s) 210, 299, 557, 571, 575
BsnI GGCC 5 cut(s) 72, 125, 330, 455, 846
Bsp120I GGGCCC 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 457
Bsp13I TCCGGA 1 cut(s) 930
Bsp143I GATC 3 cut(s) 150, 787, 808
Bsp68I TCGCGA 1 cut(s) 492
BspANI GGCC 5 cut(s) 72, 125, 330, 455, 846
BspEI TCCGGA 1 cut(s) 930
BspFNI CGCG 3 cut(s) 69, 327, 492
BspLI GGNNCC 5 cut(s) 115, 455, 559, 591, 781
BspMI ACCTGC 1 cut(s) 569
BspPI GGATC 3 cut(s) 145, 795, 816
BspQI GCTCTTC 1 cut(s) 915
BspTI CTTAAG 1 cut(s) 662
BsrFI RCCGGY 3 cut(s) 48, 302, 428
BsrI ACTGG 2 cut(s) 367, 516
BssAI RCCGGY 3 cut(s) 48, 302, 428
BssECI CCNNGG 4 cut(s) 126, 204, 325, 783
BssMI GATC 3 cut(s) 150, 787, 808
BssNAI GTATAC 1 cut(s) 799
BssNI GRCGYC 1 cut(s) 131
BssSI CACGAG 1 cut(s) 74
Bst1107I GTATAC 1 cut(s) 799
Bst2BI CACGAG 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 191, 226
Bst4CI ACNGT 4 cut(s) 148, 794, 859, 928
Bst6I CTCTTC 1 cut(s) 915
BstACI GRCGYC 1 cut(s) 131
BstAFI CTTAAG 1 cut(s) 662
BstAPI GCANNNNNTGC 1 cut(s) 28
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 7
BstDSI CCRYGG 1 cut(s) 325
BstEII GGTNACC 1 cut(s) 469
BstFNI CGCG 3 cut(s) 69, 327, 492
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 186, 353
BstKTI GATC 3 cut(s) 153, 790, 811
BstMAI GTCTC 3 cut(s) 388, 395, 514
BstMBI GATC 3 cut(s) 150, 787, 808
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 6 cut(s) 28, 78, 276, 489, 905, 959
BstNI CCWGG 2 cut(s) 191, 226
BstPI GGTNACC 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 189, 224
BstSLI GKGCMC 1 cut(s) 457
BstUI CGCG 3 cut(s) 69, 327, 492
BstV1I GCAGC 1 cut(s) 917
BstV2I GAAGAC 2 cut(s) 65, 156
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
BstZ17I GTATAC 1 cut(s) 799
BsuRI GGCC 5 cut(s) 72, 125, 330, 455, 846
BtgI CCRYGG 1 cut(s) 325
BtgZI GCGATG 2 cut(s) 507, 625
BtrI CACGTC 1 cut(s) 445
BtsI GCAGTG 2 cut(s) 16, 22
BtsIMutI CAGTG 3 cut(s) 16, 22, 790
BtuMI TCGCGA 1 cut(s) 492
BveI ACCTGC 1 cut(s) 569
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 3 cut(s) 83, 186, 353
Cfr10I RCCGGY 3 cut(s) 48, 302, 428
Cfr13I GGNCC 5 cut(s) 228, 453, 454, 558, 589
Cfr42I CCGCGG 1 cut(s) 328
CseI GACGC 4 cut(s) 82, 120, 296, 301
Csp6I GTAC 1 cut(s) 37
CviQI GTAC 1 cut(s) 37
DpnI GATC 3 cut(s) 152, 789, 810
DpnII GATC 3 cut(s) 150, 787, 808
DrdI GACNNNNNNGTC 1 cut(s) 587
DseDI GACNNNNNNGTC 1 cut(s) 587
EaeI YGGCCR 2 cut(s) 70, 328
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
EciI GGCGGA 3 cut(s) 244, 502, 595
Eco24I GRGCYC 1 cut(s) 457
Eco47I GGWCC 3 cut(s) 228, 558, 589
Eco57I CTGAAG 1 cut(s) 797
Eco72I CACGTG 1 cut(s) 55
Eco91I GGTNACC 1 cut(s) 469
EcoO109I RGGNCCY 3 cut(s) 453, 558, 589
EcoO65I GGTNACC 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 189, 224
EcoT38I GRGCYC 1 cut(s) 457
Esp3I CGTCTC 3 cut(s) 388, 395, 514
FaiI YATR 7 cut(s) 635, 705, 734, 768, 799, 827, 849
FaqI GGGAC 5 cut(s) 210, 299, 557, 571, 575
FblI GTMKAC 1 cut(s) 798
Fnu4HI GCNGC 5 cut(s) 32, 70, 187, 328, 906
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 5 cut(s) 32, 70, 187, 328, 906
FspBI CTAG 2 cut(s) 173, 650
GlaI GCGC 3 cut(s) 82, 185, 352
GluI GCNGC 5 cut(s) 32, 70, 187, 328, 906
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 5 cut(s) 72, 125, 330, 455, 846
HapII CCGG 6 cut(s) 49, 303, 387, 429, 467, 931
HgaI GACGC 4 cut(s) 82, 120, 296, 301
HhaI GCGC 3 cut(s) 83, 186, 353
Hin1I GRCGYC 1 cut(s) 131
Hin6I GCGC 3 cut(s) 81, 184, 351
HinP1I GCGC 3 cut(s) 81, 184, 351
HinfI GANTC 3 cut(s) 236, 406, 829
HpaII CCGG 6 cut(s) 49, 303, 387, 429, 467, 931
HphI GGTGA 2 cut(s) 481, 869
Hpy166II GTNNAC 1 cut(s) 799
Hpy188I TCNGA 9 cut(s) 205, 217, 235, 262, 337, 411, 784, 834, 886
Hpy188III TCNNGA 8 cut(s) 316, 424, 491, 521, 650, 694, 931, 944
Hpy8I GTNNAC 1 cut(s) 799
Hpy99I CGWCG 7 cut(s) 113, 136, 266, 401, 527, 685, 886
HpyAV CCTTC 3 cut(s) 74, 691, 836
HpyCH4III ACNGT 4 cut(s) 148, 794, 859, 928
HpyCH4IV ACGT 2 cut(s) 54, 444
HpyCH4V TGCA 3 cut(s) 534, 672, 732
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 78, 276, 489, 905, 959
HpySE526I ACGT 2 cut(s) 54, 444
Hsp92I GRCGYC 1 cut(s) 131
HspAI GCGC 3 cut(s) 81, 184, 351
Kpn2I TCCGGA 1 cut(s) 930
KspI CCGCGG 1 cut(s) 328
Kzo9I GATC 3 cut(s) 150, 787, 808
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 2 cut(s) 257, 967
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 2 cut(s) 219, 288
MaeI CTAG 2 cut(s) 173, 650
MaeII ACGT 2 cut(s) 54, 444
MaeIII GTNAC 4 cut(s) 469, 518, 751, 875
MalI GATC 3 cut(s) 152, 789, 810
MboI GATC 3 cut(s) 150, 787, 808
MboII GAAGA 4 cut(s) 70, 156, 803, 902
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 1 cut(s) 457
MluCI AATT 5 cut(s) 98, 576, 629, 655, 955
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 7 cut(s) 129, 240, 258, 285, 360, 396, 864
MroI TCCGGA 1 cut(s) 930
MseI TTAA 1 cut(s) 663
MspA1I CMGCKG 1 cut(s) 327
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 6 cut(s) 49, 303, 387, 429, 467, 931
MspR9I CCNGG 2 cut(s) 191, 226
MvaI CCWGG 2 cut(s) 191, 226
MvnI CGCG 3 cut(s) 69, 327, 492
MwoI GCNNNNNNNGC 6 cut(s) 28, 78, 276, 489, 905, 959
NdeII GATC 3 cut(s) 150, 787, 808
NlaIV GGNNCC 5 cut(s) 115, 455, 559, 591, 781
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 4 cut(s) 469, 518, 751, 875
NruI TCGCGA 1 cut(s) 492
PciSI GCTCTTC 1 cut(s) 915
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 2 cut(s) 406, 829
PkrI GCNGC 5 cut(s) 33, 71, 188, 329, 907
PleI GAGTC 1 cut(s) 230
PmaCI CACGTG 1 cut(s) 55
PmlI CACGTG 1 cut(s) 55
PpsI GAGTC 1 cut(s) 230
Ppu21I YACGTR 1 cut(s) 55
PpuMI RGGWCCY 2 cut(s) 558, 589
Psp5II RGGWCCY 2 cut(s) 558, 589
Psp6I CCWGG 2 cut(s) 189, 224
PspCI CACGTG 1 cut(s) 55
PspEI GGTNACC 1 cut(s) 469
PspGI CCWGG 2 cut(s) 189, 224
PspN4I GGNNCC 5 cut(s) 115, 455, 559, 591, 781
PspOMI GGGCCC 1 cut(s) 453
PspPI GGNCC 5 cut(s) 228, 453, 454, 558, 589
PspPPI RGGWCCY 2 cut(s) 558, 589
PsuI RGATCY 1 cut(s) 808
RruI TCGCGA 1 cut(s) 492
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SacII CCGCGG 1 cut(s) 328
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 1 cut(s) 663
SatI GCNGC 5 cut(s) 32, 70, 187, 328, 906
Sau3AI GATC 3 cut(s) 150, 787, 808
Sau96I GGNCC 5 cut(s) 228, 453, 454, 558, 589
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 191, 226
SduI GDGCHC 1 cut(s) 457
SfaNI GCATC 2 cut(s) 219, 288
Sfr303I CCGCGG 1 cut(s) 328
SgrAI CRCCGGYG 1 cut(s) 302
SgrBI CCGCGG 1 cut(s) 328
SinI GGWCC 3 cut(s) 228, 558, 589
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 5 cut(s) 98, 576, 629, 655, 955
SspMI CTAG 2 cut(s) 173, 650
StyD4I CCNGG 2 cut(s) 189, 224
TaaI ACNGT 4 cut(s) 148, 794, 859, 928
TaiI ACGT 2 cut(s) 57, 447
TaqI TCGA 3 cut(s) 396, 680, 818
TasI AATT 5 cut(s) 98, 576, 629, 655, 955
TauI GCSGC 4 cut(s) 34, 72, 189, 330
TfiI GAWTC 2 cut(s) 406, 829
Tru1I TTAA 1 cut(s) 663
Tru9I TTAA 1 cut(s) 663
TscAI CASTG 3 cut(s) 16, 22, 797
TseFI GTSAC 4 cut(s) 469, 518, 751, 875
TseI GCWGC 1 cut(s) 905
Tsp45I GTSAC 4 cut(s) 469, 518, 751, 875
TspDTI ATGAA 1 cut(s) 622
TspGWI ACGGA 3 cut(s) 126, 466, 639
TspRI CASTG 3 cut(s) 16, 22, 797
Vha464I CTTAAG 1 cut(s) 662
VpaK11BI GGWCC 3 cut(s) 228, 558, 589
XapI RAATTY 2 cut(s) 98, 629
XbaI TCTAGA 1 cut(s) 649
XmiI GTMKAC 1 cut(s) 798
XspI CTAG 2 cut(s) 173, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.