MD15G1009800.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010321
Physical Location & Seq
Reverse (-)
526553 .. 531502
4950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1009800.v1.1.491

Sequence Viewer

Length: 1728 bp
ATGCAAAACGGGGATTACTTGTCGGCTCCGTATTACCAGTACCCCCCTCCACATCAAAACCCTAATCCTATCCCCAACCCCGCCGATCACCACCACAACCCCTACGCCTCCGCACCGCCTTTCACCTCCTCCGATTACTCCCCTTATTCCCAATCTTACCCCCCATATTCTCTGAATCCCGATCCTGCCCCTCCCACCGCTCCTTCCTTCAACCCTCCCCCTCCATTCTCCAATCCCAATTTGCAAACCTTCAATCCCACGCCACAGCCGCCTTCTTCTTTCCCTCCGTTCGAGACCCATTCGCCCTATCAACCGCCGCCACAGCAGCAACCTTATCAACCACCGTACGAATATAACCAATCGGCTCCCAGTTACGCCACTTTGCCGCCCTCGATTCCCGCCAACCCTAACCCTAATTCCAATCCCTCCTCTCCGTTTTCGTCCGTTTACCAGAGCCCGTTTTCTCAACCTCCCCCTTCGGTGCCGCCTGTATATGAAAATTCTTACGAGAATTCTCTGAAATTTGATCACGGCGGTGGTTCCGGCGGCTCCTATTTAGATGATAGGTATGGAGGATATAACCGAACCCGAACCGATTTGGGATCGGATCCATATGGGAAGCGGTATGATGCAGGTCTGGATGCGGGGTACGGTGACGGTGTTTATGCTTACGGAGGTAACAAGGTGGAGCCGTATGGAGCTCGTGGCACTGCACCCAAGTCTTCAAGCTCGACTTTGTTTGATGATTACGGAAGGTCGATCAGTGTCCCATCTGGGAAAAATACGCCGGTGAGCTCGACCAAGATCGTCCGGGCAGTACCCAAGGCCGATACTCAGGAGGATGCAAAGAGTGGTGTGCAGAAGTTTCGGGTCAAGCTGTTGGCTGAAAGTGGAGGGCAGAGCACCATGGATGTCCTTTGCCAGATTGGTTTGGATGGTATCCGCATGCTTGATCCTGCTACCAGTCGGACATTGAGAATATATCCCCTCGAGACCGTTACAAGATGTGATAAAACCGACTCATCCACCTTTGCCTTTTGGTCAAAGAGTTCTGTGGATGTTGAGCCAAGGCGTATTAGATTGCAGTCAAATAGTTACACTACCAACACCCTTTTGGATACGGTGACTGCTGCAACTGTACAGCTTAAGGAAATGGGTGGAAGAATCAAGCCAACGGAATCCACAAGGCCAAGTGAACAGTCTATAGAGAGAAAGAAAGGATTAACCGATTGGATGAACATTATTAAACCGGGAAATGAGGAGAAAGATCATTGGGTCCCTGATGAAGCAGTTACAAAATGCACGGCGTGTGGGACAGATTTTGGGGCTTTTAACCGCAGGCATCACTGCCGGAACTGTGGAGACATTTTCTGCGACAAGTGTACCCATGGCAGAATTGCCTTAACTGCTGAGGAGAATGCTCCGCAGGTTCGAGTTTGTGACCGTTGCATGGCTGAAGTGACTCAGAGGCTCAGTAATGCTAAGGAAGCATCTAGTAAACCTGCAGGACTTCATAGTCACGAGGATCTTGCCAAGAAGCTTCAGGAGGAGTTGGAAAGGAATCGCAAGGAATCTTCAGGTTCAAAGTCTGATGGATCTGGAAGGAGAATGAGAGAAGTTGCCTGTCCTACATGCACGGTCCACTTGCAGGTTCAAGTTCCCAGCTCGGGGTCGGAGACCATTGAGTGTGGGGTTTGCCAGAATCCATTTCTTGTGAGTGCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYVE PTB

Protein Analysis

576

Amino Acids

62.96

Weight (kDa)

6.41

Isoelectric Point (pI)

60.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 424 - 488 1.5e-20 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1515
Acc36I ACCTGC 4 cut(s) 623, 1417, 1510, 1639
AccB1I GGYRCC 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 200
AclWI GGATC 7 cut(s) 176, 602, 610, 615, 947, 1533, 1603
AcsI RAATTY 3 cut(s) 499, 511, 521
AcuI CTGAAG 3 cut(s) 1476, 1526, 1560
AdeI CACNNNGTG 1 cut(s) 1308
AfaI GTAC 6 cut(s) 41, 347, 650, 819, 1140, 1384
AfiI CCNNNNNNNGG 4 cut(s) 1450, 1648, 1667, 1668
AflII CTTAAG 1 cut(s) 1145
AgsI TTSAA 5 cut(s) 211, 253, 726, 1584, 1655
AleI CACNNNNGTG 1 cut(s) 534
AluBI AGCT 7 cut(s) 701, 729, 795, 877, 1144, 1540, 1665
AluI AGCT 7 cut(s) 701, 729, 795, 877, 1144, 1540, 1665
Alw21I GWGCWC 4 cut(s) 703, 797, 905, 1723
Alw26I GTCTC 4 cut(s) 287, 986, 1356, 1670
AlwI GGATC 7 cut(s) 176, 602, 610, 615, 947, 1533, 1603
Ama87I CYCGRG 2 cut(s) 989, 1666
AoxI GGCC 2 cut(s) 825, 1187
ApeKI GCWGC 2 cut(s) 325, 1130
ApoI RAATTY 3 cut(s) 499, 511, 521
AspS9I GGNCC 2 cut(s) 1276, 1639
AsuC2I CCSGG 2 cut(s) 812, 1251
AsuHPI GGTGA 5 cut(s) 80, 115, 665, 802, 1135
AvaI CYCGRG 2 cut(s) 989, 1666
AvaII GGWCC 2 cut(s) 1276, 1639
BaeI ACNNNNGTAYC 2 cut(s) 1366, 1399
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 3 cut(s) 458, 703, 797
BauI CACGAG 2 cut(s) 702, 1520
BbsI GAAGAC 1 cut(s) 714
Bbv12I GWGCWC 4 cut(s) 703, 797, 905, 1723
BbvCI CCTCAGC 1 cut(s) 1410
BbvI GCAGC 2 cut(s) 337, 1117
BccI CCATC 3 cut(s) 778, 929, 1586
BceAI ACGGC 3 cut(s) 547, 676, 1320
BcgI CGANNNNNNTGC 4 cut(s) 848, 882, 1511, 1545
BciVI GTATCC 2 cut(s) 950, 1111
BclI TGATCA 1 cut(s) 526
BcnI CCSGG 2 cut(s) 812, 1251
BcoDI GTCTC 4 cut(s) 287, 986, 1356, 1670
BfaI CTAG 1 cut(s) 1494
BfmI CTRYAG 2 cut(s) 1203, 1503
BfrI CTTAAG 1 cut(s) 1145
BfuAI ACCTGC 4 cut(s) 623, 1417, 1510, 1639
BfuI GTATCC 2 cut(s) 950, 1111
BisI GCNGC 7 cut(s) 269, 317, 326, 386, 485, 547, 1131
BlsI GCNGC 7 cut(s) 270, 318, 327, 387, 486, 548, 1132
Bme1390I CCNGG 2 cut(s) 812, 1251
Bme18I GGWCC 2 cut(s) 1276, 1639
BmeT110I CYCGRG 2 cut(s) 989, 1666
BmgT120I GGNCC 2 cut(s) 1276, 1639
BmiI GGNNCC 9 cut(s) 27, 366, 483, 541, 550, 609, 690, 1277, 1278
BmrFI CCNGG 2 cut(s) 812, 1251
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 5 cut(s) 619, 631, 832, 1351, 1499
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 714
Bpu10I CCTNAGC 2 cut(s) 1410, 1482
BpuMI CCSGG 2 cut(s) 812, 1251
BsaI GGTCTC 3 cut(s) 287, 986, 1670
BsaJI CCNNGG 4 cut(s) 822, 906, 1067, 1387
Bsc4I CCNNNNNNNGG 4 cut(s) 1450, 1648, 1667, 1668
Bse118I RCCGGY 1 cut(s) 787
Bse1I ACTGG 3 cut(s) 37, 369, 963
BseDI CCNNGG 4 cut(s) 822, 906, 1067, 1387
BseGI GGATG 7 cut(s) 646, 847, 916, 940, 1022, 1063, 1239
BseLI CCNNNNNNNGG 4 cut(s) 1450, 1648, 1667, 1668
BseMII CTCAG 4 cut(s) 848, 1401, 1478, 1486
BseNI ACTGG 3 cut(s) 37, 369, 963
BseRI GAGGAG 5 cut(s) 118, 418, 1274, 1427, 1562
BseXI GCAGC 2 cut(s) 337, 1117
BseYI CCCAGC 1 cut(s) 1661
BsgI GTGCAG 2 cut(s) 696, 878
BshFI GGCC 2 cut(s) 827, 1189
BshNI GGYRCC 1 cut(s) 481
BsiHKAI GWGCWC 4 cut(s) 703, 797, 905, 1723
BsiHKCI CYCGRG 2 cut(s) 989, 1666
BsiSI CCGG 5 cut(s) 543, 788, 811, 1250, 1351
BsiWI CGTACG 1 cut(s) 345
BslFI GGGAC 3 cut(s) 752, 1262, 1327
BslI CCNNNNNNNGG 4 cut(s) 1450, 1648, 1667, 1668
BsmAI GTCTC 4 cut(s) 287, 986, 1356, 1670
BsmFI GGGAC 3 cut(s) 752, 1262, 1327
BsmI GAATGC 1 cut(s) 1423
BsnI GGCC 2 cut(s) 827, 1189
Bso31I GGTCTC 3 cut(s) 287, 986, 1670
BsoBI CYCGRG 2 cut(s) 989, 1666
Bsp1286I GDGCHC 5 cut(s) 458, 703, 797, 905, 1723
Bsp1407I TGTACA 1 cut(s) 1138
Bsp19I CCATGG 2 cut(s) 906, 1387
BspANI GGCC 2 cut(s) 827, 1189
BspCNI CTCAG 4 cut(s) 847, 1402, 1477, 1485
BspLI GGNNCC 9 cut(s) 27, 366, 483, 541, 550, 609, 690, 1277, 1278
BspMAI CTGCAG 1 cut(s) 1507
BspMI ACCTGC 4 cut(s) 623, 1417, 1510, 1639
BspPI GGATC 7 cut(s) 176, 602, 610, 615, 947, 1533, 1603
BspT107I GGYRCC 1 cut(s) 481
BspTI CTTAAG 1 cut(s) 1145
BspTNI GGTCTC 3 cut(s) 287, 986, 1670
BsrBI CCGCTC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 787
BsrGI TGTACA 1 cut(s) 1138
BsrI ACTGG 3 cut(s) 37, 369, 963
BssAI RCCGGY 1 cut(s) 787
BssECI CCNNGG 4 cut(s) 822, 906, 1067, 1387
BssSI CACGAG 2 cut(s) 702, 1520
BssT1I CCWWGG 4 cut(s) 822, 906, 1067, 1387
Bst2BI CACGAG 2 cut(s) 702, 1520
BstAFI CTTAAG 1 cut(s) 1145
BstAUI TGTACA 1 cut(s) 1138
BstC8I GCNNGC 2 cut(s) 947, 1340
BstDEI CTNAG 5 cut(s) 834, 1410, 1464, 1472, 1482
BstDSI CCRYGG 2 cut(s) 906, 1387
BstF5I GGATG 7 cut(s) 646, 847, 916, 940, 1022, 1063, 1239
BstMAI GTCTC 4 cut(s) 287, 986, 1356, 1670
BstMWI GCNNNNNNNGC 5 cut(s) 268, 322, 325, 1406, 1487
BstNSI RCATGY 2 cut(s) 949, 1635
BstSCI CCNGG 2 cut(s) 810, 1249
BstSFI CTRYAG 2 cut(s) 1203, 1503
BstV1I GCAGC 2 cut(s) 337, 1117
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 3 cut(s) 607, 1525, 1595
BstYI RGATCY 3 cut(s) 607, 1525, 1595
BsuI GTATCC 2 cut(s) 950, 1111
BsuRI GGCC 2 cut(s) 827, 1189
BtgI CCRYGG 2 cut(s) 906, 1387
BtsCI GGATG 7 cut(s) 646, 847, 916, 940, 1022, 1063, 1239
BtsI GCAGTG 2 cut(s) 708, 1345
BtsIMutI CAGTG 3 cut(s) 708, 769, 1345
BveI ACCTGC 4 cut(s) 623, 1417, 1510, 1639
Cac8I GCNNGC 2 cut(s) 947, 1340
Cfr10I RCCGGY 1 cut(s) 787
Cfr13I GGNCC 2 cut(s) 1276, 1639
Csp6I GTAC 6 cut(s) 40, 346, 649, 818, 1139, 1383
CviAII CATG 5 cut(s) 907, 946, 1388, 1450, 1632
CviQI GTAC 6 cut(s) 40, 346, 649, 818, 1139, 1383
DdeI CTNAG 5 cut(s) 834, 1410, 1464, 1472, 1482
DraIII CACNNNGTG 1 cut(s) 1308
DrdI GACNNNNNNGTC 1 cut(s) 1515
DseDI GACNNNNNNGTC 1 cut(s) 1515
Ecl136II GAGCTC 2 cut(s) 701, 795
Eco130I CCWWGG 4 cut(s) 822, 906, 1067, 1387
Eco24I GRGCYC 3 cut(s) 458, 703, 797
Eco31I GGTCTC 3 cut(s) 287, 986, 1670
Eco47I GGWCC 2 cut(s) 1276, 1639
Eco53kI GAGCTC 2 cut(s) 701, 795
Eco57I CTGAAG 3 cut(s) 1476, 1526, 1560
Eco88I CYCGRG 2 cut(s) 989, 1666
EcoICRI GAGCTC 2 cut(s) 701, 795
EcoO109I RGGNCCY 1 cut(s) 1276
EcoRI GAATTC 1 cut(s) 511
EcoT14I CCWWGG 4 cut(s) 822, 906, 1067, 1387
EcoT38I GRGCYC 3 cut(s) 458, 703, 797
ErhI CCWWGG 4 cut(s) 822, 906, 1067, 1387
FaeI CATG 5 cut(s) 910, 949, 1391, 1453, 1635
FalI AAGNNNNNCTT 2 cut(s) 718, 750
FaqI GGGAC 3 cut(s) 752, 1262, 1327
FatI CATG 5 cut(s) 906, 945, 1387, 1449, 1631
FauI CCCGC 3 cut(s) 88, 406, 637
FauNDI CATATG 1 cut(s) 613
FbaI TGATCA 1 cut(s) 526
Fnu4HI GCNGC 7 cut(s) 269, 317, 326, 386, 485, 547, 1131
FokI GGATG 7 cut(s) 653, 854, 923, 947, 1009, 1070, 1246
FriOI GRGCYC 3 cut(s) 458, 703, 797
Fsp4HI GCNGC 7 cut(s) 269, 317, 326, 386, 485, 547, 1131
FspBI CTAG 1 cut(s) 1494
GluI GCNGC 7 cut(s) 269, 317, 326, 386, 485, 547, 1131
GsaI CCCAGC 1 cut(s) 1665
HaeIII GGCC 2 cut(s) 827, 1189
HapII CCGG 5 cut(s) 543, 788, 811, 1250, 1351
Hin1II CATG 5 cut(s) 910, 949, 1391, 1453, 1635
HindIII AAGCTT 1 cut(s) 1538
HinfI GANTC 9 cut(s) 175, 394, 1019, 1164, 1178, 1462, 1561, 1571, 1702
HpaII CCGG 5 cut(s) 543, 788, 811, 1250, 1351
HphI GGTGA 5 cut(s) 80, 115, 665, 802, 1135
Hpy166II GTNNAC 5 cut(s) 448, 1196, 1383, 1499, 1642
Hpy188I TCNGA 8 cut(s) 133, 174, 519, 607, 969, 1467, 1591, 1675
Hpy188III TCNNGA 8 cut(s) 179, 292, 638, 836, 991, 1520, 1544, 1599
Hpy8I GTNNAC 5 cut(s) 448, 1196, 1383, 1499, 1642
HpyAV CCTTC 7 cut(s) 213, 217, 259, 282, 486, 747, 1596
HpyF10VI GCNNNNNNNGC 5 cut(s) 268, 322, 325, 1406, 1487
HpyF3I CTNAG 5 cut(s) 834, 1410, 1464, 1472, 1482
Hsp92II CATG 5 cut(s) 910, 949, 1391, 1453, 1635
KflI GGGWCCC 1 cut(s) 1276
Ksp22I TGATCA 1 cut(s) 526
LmnI GCTCC 7 cut(s) 31, 205, 370, 554, 688, 698, 1426
Lsp1109I GCAGC 2 cut(s) 337, 1117
LweI GCATC 5 cut(s) 619, 631, 832, 1351, 1499
MaeI CTAG 1 cut(s) 1494
MbiI CCGCTC 1 cut(s) 200
MboII GAAGA 4 cut(s) 267, 714, 1173, 1566
MflI RGATCY 3 cut(s) 607, 1525, 1595
MhlI GDGCHC 5 cut(s) 458, 703, 797, 905, 1723
MluCI AATT 6 cut(s) 238, 415, 499, 511, 521, 1395
MlyI GAGTC 2 cut(s) 1013, 1456
MmeI TCCRAC 3 cut(s) 947, 1533, 1653
MseI TTAA 5 cut(s) 1146, 1223, 1245, 1332, 1403
MslI CAYNNNNRTG 1 cut(s) 534
MspCI CTTAAG 1 cut(s) 1145
MspI CCGG 5 cut(s) 543, 788, 811, 1250, 1351
MspR9I CCNGG 2 cut(s) 812, 1251
Mva1269I GAATGC 1 cut(s) 1423
MwoI GCNNNNNNNGC 5 cut(s) 268, 322, 325, 1406, 1487
NciI CCSGG 2 cut(s) 812, 1251
NcoI CCATGG 2 cut(s) 906, 1387
NdeI CATATG 1 cut(s) 613
NlaIII CATG 5 cut(s) 910, 949, 1391, 1453, 1635
NlaIV GGNNCC 9 cut(s) 27, 366, 483, 541, 550, 609, 690, 1277, 1278
NmuCI GTSAC 5 cut(s) 653, 1123, 1439, 1459, 1517
NspI RCATGY 2 cut(s) 949, 1635
OliI CACNNNNGTG 1 cut(s) 534
PaeI GCATGC 1 cut(s) 949
PaeR7I CTCGAG 1 cut(s) 989
PctI GAATGC 1 cut(s) 1423
PfeI GAWTC 7 cut(s) 175, 394, 1164, 1178, 1561, 1571, 1702
Pfl23II CGTACG 1 cut(s) 345
PkrI GCNGC 7 cut(s) 270, 318, 327, 387, 486, 548, 1132
PleI GAGTC 2 cut(s) 1013, 1456
PpsI GAGTC 2 cut(s) 1013, 1456
PpuMI RGGWCCY 1 cut(s) 1276
Psp124BI GAGCTC 2 cut(s) 703, 797
Psp5II RGGWCCY 1 cut(s) 1276
PspFI CCCAGC 1 cut(s) 1661
PspLI CGTACG 1 cut(s) 345
PspN4I GGNNCC 9 cut(s) 27, 366, 483, 541, 550, 609, 690, 1277, 1278
PspPI GGNCC 2 cut(s) 1276, 1639
PspPPI RGGWCCY 1 cut(s) 1276
PstI CTGCAG 1 cut(s) 1507
PsuI RGATCY 3 cut(s) 607, 1525, 1595
RsaI GTAC 6 cut(s) 41, 347, 650, 819, 1140, 1384
RsaNI GTAC 6 cut(s) 40, 346, 649, 818, 1139, 1383
RseI CAYNNNNRTG 1 cut(s) 534
SacI GAGCTC 2 cut(s) 703, 797
SaqAI TTAA 5 cut(s) 1146, 1223, 1245, 1332, 1403
SatI GCNGC 7 cut(s) 269, 317, 326, 386, 485, 547, 1131
Sau96I GGNCC 2 cut(s) 1276, 1639
SbfI CCTGCAGG 1 cut(s) 1507
SchI GAGTC 2 cut(s) 1013, 1456
ScrFI CCNGG 2 cut(s) 812, 1251
SdaI CCTGCAGG 1 cut(s) 1507
SduI GDGCHC 5 cut(s) 458, 703, 797, 905, 1723
SfaNI GCATC 5 cut(s) 619, 631, 832, 1351, 1499
SfcI CTRYAG 2 cut(s) 1203, 1503
Sfr274I CTCGAG 1 cut(s) 989
SgrAI CRCCGGYG 1 cut(s) 787
SinI GGWCC 2 cut(s) 1276, 1639
SlaI CTCGAG 1 cut(s) 989
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 2 cut(s) 989, 1145
SmoI CTYRAG 2 cut(s) 989, 1145
SphI GCATGC 1 cut(s) 949
Sse8387I CCTGCAGG 1 cut(s) 1507
Sse9I AATT 6 cut(s) 238, 415, 499, 511, 521, 1395
SspMI CTAG 1 cut(s) 1494
SstI GAGCTC 2 cut(s) 703, 797
StyD4I CCNGG 2 cut(s) 810, 1249
StyI CCWWGG 4 cut(s) 822, 906, 1067, 1387
TaqI TCGA 7 cut(s) 291, 392, 731, 758, 797, 990, 1432
TasI AATT 6 cut(s) 238, 415, 499, 511, 521, 1395
TatI WGTACW 1 cut(s) 1138
TauI GCSGC 5 cut(s) 271, 319, 388, 487, 549
TfiI GAWTC 7 cut(s) 175, 394, 1164, 1178, 1561, 1571, 1702
Tru1I TTAA 5 cut(s) 1146, 1223, 1245, 1332, 1403
Tru9I TTAA 5 cut(s) 1146, 1223, 1245, 1332, 1403
TscAI CASTG 3 cut(s) 715, 769, 1352
TseFI GTSAC 5 cut(s) 653, 1123, 1439, 1459, 1517
TseI GCWGC 2 cut(s) 325, 1130
Tsp45I GTSAC 5 cut(s) 653, 1123, 1439, 1459, 1517
TspDTI ATGAA 4 cut(s) 510, 1250, 1299, 1502
TspGWI ACGGA 7 cut(s) 18, 276, 423, 433, 687, 765, 1190
TspRI CASTG 3 cut(s) 715, 769, 1352
Vha464I CTTAAG 1 cut(s) 1145
VpaK11BI GGWCC 2 cut(s) 1276, 1639
XapI RAATTY 3 cut(s) 499, 511, 521
XceI RCATGY 2 cut(s) 949, 1635
XcmI CCANNNNNNNNNTGG 1 cut(s) 1111
XhoI CTCGAG 1 cut(s) 989
XspI CTAG 1 cut(s) 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.