MD15G1048100.v1.1

G-protein coupled receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012013
Physical Location & Seq
Reverse (-)
3285673 .. 3289131
3459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1048100.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGGGGAGACGGGGCAAGTCGCCGGAGGTCTGACGGCGTACGATCGCGAAGTCTTGACGGCAGTTAACGCCGGAGCGGCGAGCCTATCGTTCGTCGGGTCGGGGTTCATCGTCCTCTGCTACGTCCTCTTCAAAGAGCTCCGCAAGTTCTCCTTCAAGCTCGTCTTCTACCTCGCGCTCTCCGATATGCTTTGTAGCTTCTTCAGCATGGTTGGGGATCCATCCAGAGGATTTTTCTGTTACTTGCACGGCTACAGTACTCATTTCTTCTATGTAGCGTCTTTCTTATGGACTACCACGATCGCTTTTACACTCCACCGCACAGTTGTTAGACACAAAACTGATGTTGAAGATATGGAGGCAATGTTTCATTTGTATGTTTGGGGGACTTCATTAGTTGTGACAGTTGTGCGCTCTATTGGGAATAACCATAGCCACCTAGGTACATGGTGTTGGTCACAATCAGGGCGTACTGGAAAGGCGCTTCACCTTGTAACATTTTATATGCCACTTTGGGGTGCAATTCTTTACAATGGTTTTACATATTTTCAAGTGATAAGCATGCTGAAGAATGCGAGACGTATGGCAGCTGGCATGTCAGATCGGACTTACCAATCAGATTCTAGAGCAGAAATGAAGGCTTTGAACCGGTGGGGGTACTATCCGCTCATCCTGATAGGATCATGGGCTTTTAGCACAATCAACCGCATTCATGACTTTATTGAACCAAGTCATAAAATATTTTGGCTCTCAGTTCTTGATGTTGGGACAGCTGCACTCATGGGCCTGTTCAACTCCATAGCCTATGGCTTGAACTCCTCAGTGCGACGGGCAATATATGAAAGATTGGATTTGTTCTGGCCAGACAGGCTTAAAAGATGGTTCCCCAACAGTTCAAAGTTTAGGAACCAACAGCAAGAGAGTGAACTAGTGGCGCTGACAAATGAAGATGAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0001101 GO:0003006 GO:0003674 GO:0004888 GO:0004930 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006082 GO:0006109 GO:0006520 GO:0006558 GO:0006570 GO:0006571 GO:0006725 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007186 GO:0007202 GO:0007275 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009094 GO:0009095 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009642 GO:0009645 GO:0009719 GO:0009725 GO:0009735 GO:0009737 GO:0009738 GO:0009741 GO:0009742 GO:0009755 GO:0009785 GO:0009787 GO:0009788 GO:0009791 GO:0009845 GO:0009889 GO:0009908 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010154 GO:0010162 GO:0010231 GO:0010244 GO:0010431 GO:0010517 GO:0010518 GO:0010646 GO:0010647 GO:0010648 GO:0010675 GO:0010863 GO:0010919 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016053 GO:0017144 GO:0019220 GO:0019222 GO:0019438 GO:0019752 GO:0021700 GO:0022414 GO:0022611 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030522 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0032960 GO:0033993 GO:0038023 GO:0042221 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0043401 GO:0043436 GO:0044093 GO:0044214 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046394 GO:0048316 GO:0048367 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051174 GO:0051336 GO:0051345 GO:0051716 GO:0060089 GO:0060191 GO:0060193 GO:0061458 GO:0062012 GO:0065007 GO:0065009 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071478 GO:0071482 GO:0071483 GO:0071495 GO:0071695 GO:0071704 GO:0071944 GO:0080090 GO:0089717 GO:0090351 GO:0090567 GO:0097305 GO:0097306 GO:0097437 GO:0098588 GO:0098805 GO:0104004 GO:1900274 GO:1901360 GO:1901362 GO:1901419 GO:1901420 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700 GO:1901701 GO:1902221 GO:1902223 GO:1902930 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dicty_CAR

Protein Analysis

320

Amino Acids

36.49

Weight (kDa)

8.96

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dicty_CAR PF05462 25 - 273 1.5e-14 Slime mold cyclic AMP receptor
7tm_2 PF00002 27 - 202 4.9e-08 7 transmembrane receptor (Secretin family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012013)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48270 AT1G48270
fragaria_vesca FvH4_2g30820
malus_domestica MD08G1073200.v1.1 MD15G1048100.v1.1
prunus_persica Prupe.1G402500_v2.0.a1 Prupe.1G402500_v2.0.a1
pyrus_communis pycom08g06060 pycom15g04710
rosa_chinensis RchiOBHm_Chr6g0312021
rosa_laevigata RLG00000010360
rosa_multiflora Rmu_sc0005381.1_g000008
rosa_roxburghii Rroxscaffold_7G00157120
rosa_rugosa Rorug06G0392300
rosa_samantha Rh6AG508700 Rh6BG520000 Rh6CG525900 Rh6DG511400
rosa_wichuraiana Rw6G044300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 77, 667
AccII CGCG 2 cut(s) 48, 176
AciI CCGC 5 cut(s) 77, 142, 319, 665, 706
AclWI GGATC 3 cut(s) 212, 225, 688
AcoI YGGCCR 1 cut(s) 860
AcuI CTGAAG 2 cut(s) 187, 587
AfaI GTAC 5 cut(s) 41, 259, 445, 472, 659
AfiI CCNNNNNNNGG 2 cut(s) 227, 515
AgeI ACCGGT 1 cut(s) 648
AgsI TTSAA 9 cut(s) 133, 157, 350, 551, 646, 725, 793, 814, 897
AhlI ACTAGT 1 cut(s) 928
AluBI AGCT 5 cut(s) 139, 160, 198, 590, 773
AluI AGCT 5 cut(s) 139, 160, 198, 590, 773
Alw21I GWGCWC 2 cut(s) 141, 957
Alw26I GTCTC 2 cut(s) 2, 571
AlwI GGATC 3 cut(s) 212, 225, 688
AoxI GGCC 2 cut(s) 784, 860
ApeKI GCWGC 2 cut(s) 587, 773
AsiGI ACCGGT 1 cut(s) 648
AspA2I CCTAGG 1 cut(s) 439
AspLEI GCGC 4 cut(s) 178, 414, 484, 937
AspS9I GGNCC 1 cut(s) 784
AsuHPI GGTGA 1 cut(s) 479
AvrII CCTAGG 1 cut(s) 439
BalI TGGCCA 1 cut(s) 862
BamHI GGATCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 157
Bbv12I GWGCWC 2 cut(s) 141, 957
BbvI GCAGC 2 cut(s) 599, 760
BccI CCATC 2 cut(s) 229, 873
BceAI ACGGC 3 cut(s) 51, 75, 265
BcoDI GTCTC 2 cut(s) 2, 571
BcuI ACTAGT 1 cut(s) 928
BfaI CTAG 4 cut(s) 440, 624, 929, 958
BfmI CTRYAG 1 cut(s) 253
BfoI RGCGCY 2 cut(s) 485, 938
BglI GCCNNNNNGGC 2 cut(s) 77, 868
BisI GCNGC 3 cut(s) 78, 588, 774
BlnI CCTAGG 1 cut(s) 439
BlsI GCNGC 3 cut(s) 79, 589, 775
BmcAI AGTACT 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 784
BmiI GGNNCC 3 cut(s) 219, 884, 908
BpiI GAAGAC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 439
BsaWI WCCGGW 1 cut(s) 648
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 515
Bse118I RCCGGY 1 cut(s) 648
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 2 cut(s) 221, 669
BseLI CCNNNNNNNGG 2 cut(s) 227, 515
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 765, 834
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 808
BseXI GCAGC 2 cut(s) 599, 760
BsgI GTGCAG 1 cut(s) 759
Bsh1236I CGCG 2 cut(s) 48, 176
Bsh1285I CGRYCG 2 cut(s) 46, 303
BshFI GGCC 2 cut(s) 786, 862
BshTI ACCGGT 1 cut(s) 648
BsiEI CGRYCG 2 cut(s) 46, 303
BsiHKAI GWGCWC 2 cut(s) 141, 957
BsiSI CCGG 3 cut(s) 24, 72, 649
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 2 cut(s) 400, 781
BslI CCNNNNNNNGG 2 cut(s) 227, 515
BsmAI GTCTC 2 cut(s) 2, 571
BsmBI CGTCTC 2 cut(s) 2, 571
BsmFI GGGAC 2 cut(s) 400, 781
BsmI GAATGC 2 cut(s) 577, 708
BsnI GGCC 2 cut(s) 786, 862
Bsp1286I GDGCHC 2 cut(s) 141, 957
Bsp143I GATC 5 cut(s) 43, 217, 300, 601, 680
Bsp68I TCGCGA 1 cut(s) 48
BspACI CCGC 5 cut(s) 77, 142, 319, 665, 706
BspANI GGCC 2 cut(s) 786, 862
BspCNI CTCAG 2 cut(s) 764, 833
BspFNI CGCG 2 cut(s) 48, 176
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 3 cut(s) 219, 884, 908
BspPI GGATC 3 cut(s) 212, 225, 688
BsrBI CCGCTC 2 cut(s) 77, 667
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 648
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 1 cut(s) 648
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 5 cut(s) 43, 217, 300, 601, 680
BssT1I CCWWGG 1 cut(s) 439
Bst4CI ACNGT 4 cut(s) 257, 325, 406, 893
Bst6I CTCTTC 1 cut(s) 134
BstC8I GCNNGC 3 cut(s) 82, 563, 592
BstDEI CTNAG 2 cut(s) 751, 820
BstF5I GGATG 2 cut(s) 221, 669
BstFNI CGCG 2 cut(s) 48, 176
BstH2I RGCGCY 2 cut(s) 485, 938
BstHHI GCGC 4 cut(s) 178, 414, 484, 937
BstKTI GATC 5 cut(s) 46, 220, 303, 604, 683
BstMAI GTCTC 2 cut(s) 2, 571
BstMBI GATC 5 cut(s) 43, 217, 300, 601, 680
BstMCI CGRYCG 2 cut(s) 46, 303
BstMWI GCNNNNNNNGC 4 cut(s) 68, 77, 204, 868
BstNSI RCATGY 2 cut(s) 565, 598
BstSFI CTRYAG 1 cut(s) 253
BstUI CGCG 2 cut(s) 48, 176
BstV1I GCAGC 2 cut(s) 599, 760
BstV2I GAAGAC 1 cut(s) 157
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 2 cut(s) 786, 862
BtsCI GGATG 2 cut(s) 221, 669
BtsIMutI CAGTG 1 cut(s) 828
BtuMI TCGCGA 1 cut(s) 48
Cac8I GCNNGC 3 cut(s) 82, 563, 592
CciI TCATGA 1 cut(s) 712
CfoI GCGC 4 cut(s) 178, 414, 484, 937
Cfr10I RCCGGY 1 cut(s) 648
Cfr13I GGNCC 1 cut(s) 784
CseI GACGC 1 cut(s) 267
Csp6I GTAC 5 cut(s) 40, 258, 444, 471, 658
CspAI ACCGGT 1 cut(s) 648
CviAII CATG 7 cut(s) 208, 447, 562, 595, 684, 713, 781
CviQI GTAC 5 cut(s) 40, 258, 444, 471, 658
DdeI CTNAG 2 cut(s) 751, 820
DpnI GATC 5 cut(s) 45, 219, 302, 603, 682
DpnII GATC 5 cut(s) 43, 217, 300, 601, 680
EaeI YGGCCR 1 cut(s) 860
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 187, 587
EcoICRI GAGCTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 439
Esp3I CGTCTC 2 cut(s) 2, 571
FaeI CATG 7 cut(s) 211, 450, 565, 598, 687, 716, 784
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 149, 181
FaqI GGGAC 2 cut(s) 400, 781
FatI CATG 7 cut(s) 207, 446, 561, 594, 683, 712, 780
Fnu4HI GCNGC 3 cut(s) 78, 588, 774
FokI GGATG 2 cut(s) 208, 656
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 3 cut(s) 78, 588, 774
FspBI CTAG 4 cut(s) 440, 624, 929, 958
GlaI GCGC 4 cut(s) 177, 413, 483, 936
GluI GCNGC 3 cut(s) 78, 588, 774
HaeII RGCGCY 2 cut(s) 485, 938
HaeIII GGCC 2 cut(s) 786, 862
HapII CCGG 3 cut(s) 24, 72, 649
HgaI GACGC 1 cut(s) 267
HhaI GCGC 4 cut(s) 178, 414, 484, 937
Hin1II CATG 7 cut(s) 211, 450, 565, 598, 687, 716, 784
Hin6I GCGC 4 cut(s) 176, 412, 482, 935
HinP1I GCGC 4 cut(s) 176, 412, 482, 935
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 1 cut(s) 620
HpaI GTTAAC 1 cut(s) 67
HpaII CCGG 3 cut(s) 24, 72, 649
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 2 cut(s) 67, 926
Hpy188I TCNGA 5 cut(s) 33, 184, 601, 606, 619
Hpy188III TCNNGA 7 cut(s) 47, 55, 225, 624, 673, 713, 758
Hpy8I GTNNAC 2 cut(s) 67, 926
Hpy99I CGWCG 2 cut(s) 98, 831
HpyAV CCTTC 2 cut(s) 163, 631
HpyCH4III ACNGT 4 cut(s) 257, 325, 406, 893
HpyCH4IV ACGT 2 cut(s) 123, 580
HpyCH4V TGCA 3 cut(s) 247, 521, 776
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 77, 204, 868
HpyF3I CTNAG 2 cut(s) 751, 820
HpySE526I ACGT 2 cut(s) 123, 580
Hsp92II CATG 7 cut(s) 211, 450, 565, 598, 687, 716, 784
HspAI GCGC 4 cut(s) 176, 412, 482, 935
KspAI GTTAAC 1 cut(s) 67
Kzo9I GATC 5 cut(s) 43, 217, 300, 601, 680
LmnI GCTCC 2 cut(s) 74, 144
Lsp1109I GCAGC 2 cut(s) 599, 760
MaeI CTAG 4 cut(s) 440, 624, 929, 958
MaeII ACGT 2 cut(s) 123, 580
MaeIII GTNAC 4 cut(s) 239, 400, 456, 493
MalI GATC 5 cut(s) 45, 219, 302, 603, 682
MbiI CCGCTC 2 cut(s) 77, 667
MboI GATC 5 cut(s) 43, 217, 300, 601, 680
MboII GAAGA 7 cut(s) 121, 157, 193, 259, 362, 580, 959
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 2 cut(s) 141, 957
MlsI TGGCCA 1 cut(s) 862
MluCI AATT 1 cut(s) 522
MluNI TGGCCA 1 cut(s) 862
MnlI CCTC 7 cut(s) 20, 125, 137, 182, 221, 352, 829
Mox20I TGGCCA 1 cut(s) 862
MscI TGGCCA 1 cut(s) 862
MseI TTAA 2 cut(s) 66, 873
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 862
MspA1I CMGCKG 2 cut(s) 590, 773
MspI CCGG 3 cut(s) 24, 72, 649
Mva1269I GAATGC 2 cut(s) 577, 708
MvnI CGCG 2 cut(s) 48, 176
MwoI GCNNNNNNNGC 4 cut(s) 68, 77, 204, 868
NdeII GATC 5 cut(s) 43, 217, 300, 601, 680
NlaIII CATG 7 cut(s) 211, 450, 565, 598, 687, 716, 784
NlaIV GGNNCC 3 cut(s) 219, 884, 908
NmuCI GTSAC 2 cut(s) 400, 456
NruI TCGCGA 1 cut(s) 48
NspI RCATGY 2 cut(s) 565, 598
PaeI GCATGC 1 cut(s) 565
PagI TCATGA 1 cut(s) 712
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 2 cut(s) 577, 708
PfeI GAWTC 1 cut(s) 620
Pfl23II CGTACG 1 cut(s) 39
PinAI ACCGGT 1 cut(s) 648
PkrI GCNGC 3 cut(s) 79, 589, 775
Ple19I CGATCG 2 cut(s) 46, 303
Psp124BI GAGCTC 1 cut(s) 141
PspLI CGTACG 1 cut(s) 39
PspN4I GGNNCC 3 cut(s) 219, 884, 908
PspPI GGNCC 1 cut(s) 784
PsuI RGATCY 1 cut(s) 217
PvuI CGATCG 2 cut(s) 46, 303
PvuII CAGCTG 2 cut(s) 590, 773
RruI TCGCGA 1 cut(s) 48
RsaI GTAC 5 cut(s) 41, 259, 445, 472, 659
RsaNI GTAC 5 cut(s) 40, 258, 444, 471, 658
RseI CAYNNNNRTG 1 cut(s) 375
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 2 cut(s) 66, 873
SatI GCNGC 3 cut(s) 78, 588, 774
Sau3AI GATC 5 cut(s) 43, 217, 300, 601, 680
Sau96I GGNCC 1 cut(s) 784
ScaI AGTACT 1 cut(s) 259
SduI GDGCHC 2 cut(s) 141, 957
SfcI CTRYAG 1 cut(s) 253
SmiMI CAYNNNNRTG 1 cut(s) 375
SpeI ACTAGT 1 cut(s) 928
SphI GCATGC 1 cut(s) 565
Sse9I AATT 1 cut(s) 522
SsiI CCGC 5 cut(s) 77, 142, 319, 665, 706
SspI AATATT 1 cut(s) 741
SspMI CTAG 4 cut(s) 440, 624, 929, 958
SstI GAGCTC 1 cut(s) 141
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 4 cut(s) 257, 325, 406, 893
TaiI ACGT 2 cut(s) 126, 583
TasI AATT 1 cut(s) 522
TatI WGTACW 1 cut(s) 257
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 620
Tru1I TTAA 2 cut(s) 66, 873
Tru9I TTAA 2 cut(s) 66, 873
TscAI CASTG 1 cut(s) 828
TseFI GTSAC 2 cut(s) 400, 456
TseI GCWGC 2 cut(s) 587, 773
Tsp45I GTSAC 2 cut(s) 400, 456
TspDTI ATGAA 7 cut(s) 97, 359, 381, 650, 701, 855, 960
TspRI CASTG 1 cut(s) 828
XbaI TCTAGA 1 cut(s) 623
XceI RCATGY 2 cut(s) 565, 598
XmaJI CCTAGG 1 cut(s) 439
XspI CTAG 4 cut(s) 440, 624, 929, 958
ZrmI AGTACT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.