Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014062

Physical Location & Seq

Genomic Coordinates Reverse (-)

3911781 .. 3914023

Gene Structure 2243 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1058400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGGCTCATTTGCTTTCAACTTCCTGCTCTCTAAAATTCTCCTCTGGTGGCAAACCATACACCAAACCATTGATCCAACACTCGATCGCATTTCCTTCCTCTTTAACTTTTCAGACTTCCAAAACCCCATTTGGGAGGATCATCCTGCAAGGGGAGAGGTCACGGAGTGGTACTTGCACGGTGCGTGCAACCACACTCTCTGCAAGTAGGGAAGCCCCAAATGAACCGAGCTACGGCGCTAATGAGAGTTCAGGCGGAGCTTCCAAACCACAGCGGGTGATGGTCATTGGTGGAGATGGCTACTGTGGTTGGGCCACAGCCCTTCACCTATCCAAGAAAGGTTATGAGGTTGCCATTGTTGATAGCCTTGTGCGCCGTCTCTTTGACCACCAGCTCGGTCTTGGTTCCCTGACTCCAATCGCCTCTATCCATGATCGTATCCGTTGTTGGAAAGCTCTCACTGGAAAGTCCATTGAGCTCTGCATTGGTGATGTTTGTGATTTCGAGTTCTTGTCAGAAGCCTTCAAGTCATTTGAACCTGATTCTGTTGTTCATTTCGGGGAACAGCGGTCTGCCCCTTACTCCATGATTGATCGGTCCAGAGCAGTATTCACGCAGAATAACAACGTGATTGGAACTCTGAATGTTCTCTTTGCTATAAAAGAGTTCAGAGAGGAGTGTCATCTCGTGAAGCTCGGGACCATGGGAGAATATGGGACTCCAAATATTGACATTGAGGAGGGTTTCATTACAATTACTCATAACGGGAGAACAGATACTTTGCCCTTCCCGAAGCAAGCGAGCTCTTTCTACCATTTGAGCAAGGTTCACGATTCGAATAATATAGCCTTCACTTGCAAGGCTTGGGGGATTAGAGCCACTGATCTAAATCAAGGAGTGGTATATGGAGTGAGAACGGATGAAACCGAGATGCATGAAGAGCTCAATAACAGGTTTGATTATGATGGAGTTTTTGGAACTGCATTAAACCGGTTCTGCGTGCAGGCTGCTGTCGGTCATCCACTTACCGTGTATGGCAAAGGGGGTCAGACCAGGGGATATCTTGACATACGAGACACAGTTCAGTGTGTTGAAATTGCCATCGCAAACCCAGCAAAGGCCGGTGAGTTTAGGGTCTTCAATCAATTTACTGAGCAGTTTTCCGTCAATCAACTTGCCGCACTTGTTACCAAAGCTGGAGAGAAGCTCGGAATTGATGTGCAAACCACCTCTGTCCCAAACCCTAGAGTTGAAGCTGAGGAGCACTACTACAACGCTAAACACACTAAACTTATTGAATTGGGACTGAAACCGCACCTTCTTTCGGACTCTCTTTTGGATTCATTGCTCAATTTTGCTGTTAGGTACAAGGATCGCGTCGACACAAAGCAGATAATGCCCAGTGTATCTTGGAGAAAAATGGGGGCCAAGCCGAAGACTGTCACAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006643 GO:0006664 GO:0006950 GO:0007154 GO:0008146 GO:0008150 GO:0008152 GO:0008270 GO:0008610 GO:0009058 GO:0009247 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016740 GO:0016782 GO:0019899 GO:0019904 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046467 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0101016 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00520 ko00561 map00520 map00561

Pfam Domains

Protein Families

Epimerase

Protein Analysis

484

Amino Acids

53.29

Weight (kDa)

8.23

Isoelectric Point (pI)

35.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	93 - 381	7.6e-22	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	94 - 396	1.5e-15	GDP-mannose 4,6 dehydratase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014062)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33030
fragaria_vesca	FvH4_2g31750
malus_domestica	MD15G1058400.v1.1
prunus_persica	Prupe.1G411000_v2.0.a1 Prupe.1G411000_v2.0.a1 Prupe.1G411000_v2.0.a1
pyrus_communis	pycom15g05490
rosa_chinensis	RchiOBHm_Chr6g0311071
rosa_laevigata	RLG00000010438
rosa_multiflora	Rmu_sc0012235.1_g000003
rosa_roxburghii	Rroxscaffold_7G00158050
rosa_rugosa	Rorug06G0385100
rosa_samantha	Rh6AG499300 Rh6BG509700 Rh6CG515500 Rh6DG502100
rosa_wichuraiana	Rw6G043480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1380
AccII	CGCG	1 cut(s)	1377
AciI	CCGC	5 cut(s)	255, 274, 568, 1179, 1313
AclWI	GGATC	3 cut(s)	67, 146, 1380
AcsI	RAATTY	1 cut(s)	35
AdeI	CACNNNGTG	1 cut(s)	167
AfaI	GTAC	2 cut(s)	172, 1367
AfiI	CCNNNNNNNGG	5 cut(s)	132, 151, 233, 1117, 1324
AgeI	ACCGGT	1 cut(s)	990
AgsI	TTSAA	7 cut(s)	18, 526, 536, 1094, 1141, 1253, 1298
AjnI	CCWGG	1 cut(s)	1052
Alw21I	GWGCWC	4 cut(s)	480, 806, 945, 1266
Alw26I	GTCTC	2 cut(s)	383, 1068
AlwI	GGATC	3 cut(s)	67, 146, 1380
Ama87I	CYCGRG	1 cut(s)	695
AoxI	GGCC	3 cut(s)	312, 1119, 1425
ApeKI	GCWGC	1 cut(s)	1007
ApoI	RAATTY	1 cut(s)	35
AsiGI	ACCGGT	1 cut(s)	990
AspLEI	GCGC	2 cut(s)	239, 375
AspS9I	GGNCC	4 cut(s)	312, 597, 699, 1425
AsuHPI	GGTGA	4 cut(s)	289, 317, 500, 1136
AsuII	TTCGAA	1 cut(s)	836
AvaI	CYCGRG	1 cut(s)	695
AvaII	GGWCC	2 cut(s)	597, 699
BanII	GRGCYC	3 cut(s)	480, 806, 945
BauI	CACGAG	1 cut(s)	686
BbsI	GAAGAC	2 cut(s)	1129, 1442
Bbv12I	GWGCWC	4 cut(s)	480, 806, 945, 1266
BbvCI	CCTCAGC	1 cut(s)	1257
BbvI	GCAGC	1 cut(s)	994
BccI	CCATC	4 cut(s)	274, 290, 959, 1109
BceAI	ACGGC	2 cut(s)	250, 360
BciT130I	CCWGG	1 cut(s)	1054
BciVI	GTATCC	1 cut(s)	449
BcoDI	GTCTC	2 cut(s)	383, 1068
BfaI	CTAG	1 cut(s)	1245
BfoI	RGCGCY	1 cut(s)	240
BfuI	GTATCC	1 cut(s)	449
BisI	GCNGC	2 cut(s)	1008, 1179
BlsI	GCNGC	2 cut(s)	1009, 1180
Bme1390I	CCNGG	1 cut(s)	1054
Bme18I	GGWCC	2 cut(s)	597, 699
BmeT110I	CYCGRG	1 cut(s)	695
BmgT120I	GGNCC	4 cut(s)	312, 597, 699, 1425
BmiI	GGNNCC	3 cut(s)	406, 700, 1426
BmrFI	CCNGG	1 cut(s)	1054
BmrI	ACTGGG	1 cut(s)	1395
BmsI	GCATC	1 cut(s)	921
BmuI	ACTGGG	1 cut(s)	1395
BpiI	GAAGAC	2 cut(s)	1129, 1442
BplI	GAGNNNNNCTC	2 cut(s)	1191, 1223
BpmI	CTGGAG	1 cut(s)	1218
Bpu10I	CCTNAGC	1 cut(s)	1257
Bpu14I	TTCGAA	1 cut(s)	836
BsaBI	GATNNNNATC	1 cut(s)	888
BsaJI	CCNNGG	2 cut(s)	702, 1053
BsaWI	WCCGGW	1 cut(s)	990
Bsc4I	CCNNNNNNNGG	5 cut(s)	132, 151, 233, 1117, 1324
Bse118I	RCCGGY	2 cut(s)	990, 1121
Bse1I	ACTGG	2 cut(s)	466, 1401
Bse3DI	GCAATG	1 cut(s)	1343
Bse8I	GATNNNNATC	1 cut(s)	888
BseBI	CCWGG	1 cut(s)	1054
BseDI	CCNNGG	2 cut(s)	702, 1053
BseGI	GGATG	3 cut(s)	141, 925, 1018
BseJI	GATNNNNATC	1 cut(s)	888
BseLI	CCNNNNNNNGG	5 cut(s)	132, 151, 233, 1117, 1324
BseMI	GCAATG	1 cut(s)	1343
BseMII	CTCAG	2 cut(s)	1143, 1248
BseNI	ACTGG	2 cut(s)	466, 1401
BseRI	GAGGAG	4 cut(s)	31, 689, 752, 1274
BseXI	GCAGC	1 cut(s)	994
BseYI	CCCAGC	1 cut(s)	1111
BsgI	GTGCAG	1 cut(s)	1022
Bsh1236I	CGCG	1 cut(s)	1377
Bsh1285I	CGRYCG	1 cut(s)	87
BshFI	GGCC	3 cut(s)	314, 1121, 1427
BshTI	ACCGGT	1 cut(s)	990
BsiEI	CGRYCG	1 cut(s)	87
BsiHKAI	GWGCWC	4 cut(s)	480, 806, 945, 1266
BsiHKCI	CYCGRG	1 cut(s)	695
BsiSI	CCGG	2 cut(s)	991, 1122
BslFI	GGGAC	4 cut(s)	712, 730, 1220, 1317
BslI	CCNNNNNNNGG	5 cut(s)	132, 151, 233, 1117, 1324
BsmAI	GTCTC	2 cut(s)	383, 1068
BsmBI	CGTCTC	1 cut(s)	383
BsmFI	GGGAC	4 cut(s)	712, 730, 1220, 1317
BsnI	GGCC	3 cut(s)	314, 1121, 1427
BsoBI	CYCGRG	1 cut(s)	695
Bsp119I	TTCGAA	1 cut(s)	836
Bsp1286I	GDGCHC	4 cut(s)	480, 806, 945, 1266
Bsp143I	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
Bsp19I	CCATGG	1 cut(s)	702
BspACI	CCGC	5 cut(s)	255, 274, 568, 1179, 1313
BspANI	GGCC	3 cut(s)	314, 1121, 1427
BspCNI	CTCAG	2 cut(s)	1144, 1249
BspFNI	CGCG	1 cut(s)	1377
BspLI	GGNNCC	3 cut(s)	406, 700, 1426
BspPI	GGATC	3 cut(s)	67, 146, 1380
BspQI	GCTCTTC	1 cut(s)	933
BspT104I	TTCGAA	1 cut(s)	836
BsrDI	GCAATG	1 cut(s)	1343
BsrFI	RCCGGY	2 cut(s)	990, 1121
BsrI	ACTGG	2 cut(s)	466, 1401
BssAI	RCCGGY	2 cut(s)	990, 1121
BssECI	CCNNGG	2 cut(s)	702, 1053
BssMI	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
BssSI	CACGAG	1 cut(s)	686
BssT1I	CCWWGG	1 cut(s)	702
Bst2BI	CACGAG	1 cut(s)	686
Bst2UI	CCWGG	1 cut(s)	1054
Bst4CI	ACNGT	5 cut(s)	181, 305, 1030, 1081, 1441
Bst6I	CTCTTC	1 cut(s)	933
BstAPI	GCANNNNNTGC	1 cut(s)	1396
BstBI	TTCGAA	1 cut(s)	836
BstC8I	GCNNGC	5 cut(s)	186, 798, 802, 1001, 1005
BstDEI	CTNAG	2 cut(s)	1152, 1257
BstDSI	CCRYGG	1 cut(s)	702
BstF5I	GGATG	3 cut(s)	141, 925, 1018
BstFNI	CGCG	1 cut(s)	1377
BstH2I	RGCGCY	1 cut(s)	240
BstHHI	GCGC	2 cut(s)	239, 375
BstKTI	GATC	7 cut(s)	75, 87, 141, 436, 595, 886, 1375
BstMAI	GTCTC	2 cut(s)	383, 1068
BstMBI	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
BstMCI	CGRYCG	1 cut(s)	87
BstMWI	GCNNNNNNNGC	4 cut(s)	372, 940, 1112, 1396
BstNI	CCWGG	1 cut(s)	1054
BstSCI	CCNGG	1 cut(s)	1052
BstUI	CGCG	1 cut(s)	1377
BstV1I	GCAGC	1 cut(s)	994
BstV2I	GAAGAC	2 cut(s)	1129, 1442
BsuI	GTATCC	1 cut(s)	449
BsuRI	GGCC	3 cut(s)	314, 1121, 1427
BtgI	CCRYGG	1 cut(s)	702
BtgZI	GCGATG	1 cut(s)	1087
BtsCI	GGATG	3 cut(s)	141, 925, 1018
BtsIMutI	CAGTG	4 cut(s)	459, 879, 1091, 1408
Cac8I	GCNNGC	5 cut(s)	186, 798, 802, 1001, 1005
CfoI	GCGC	2 cut(s)	239, 375
Cfr10I	RCCGGY	2 cut(s)	990, 1121
Cfr13I	GGNCC	4 cut(s)	312, 597, 699, 1425
CseI	GACGC	1 cut(s)	1366
Csp6I	GTAC	2 cut(s)	171, 1366
CspAI	ACCGGT	1 cut(s)	990
CviAII	CATG	4 cut(s)	431, 586, 703, 935
CviQI	GTAC	2 cut(s)	171, 1366
DdeI	CTNAG	2 cut(s)	1152, 1257
DpnI	GATC	7 cut(s)	74, 86, 140, 435, 594, 885, 1374
DpnII	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
DraIII	CACNNNGTG	1 cut(s)	167
Eam1104I	CTCTTC	1 cut(s)	933
EarI	CTCTTC	1 cut(s)	933
EciI	GGCGGA	1 cut(s)	270
Ecl136II	GAGCTC	3 cut(s)	478, 804, 943
Eco130I	CCWWGG	1 cut(s)	702
Eco24I	GRGCYC	3 cut(s)	480, 806, 945
Eco32I	GATATC	1 cut(s)	1061
Eco47I	GGWCC	2 cut(s)	597, 699
Eco53kI	GAGCTC	3 cut(s)	478, 804, 943
Eco88I	CYCGRG	1 cut(s)	695
EcoICRI	GAGCTC	3 cut(s)	478, 804, 943
EcoRII	CCWGG	1 cut(s)	1052
EcoRV	GATATC	1 cut(s)	1061
EcoT14I	CCWWGG	1 cut(s)	702
EcoT22I	ATGCAT	1 cut(s)	936
EcoT38I	GRGCYC	3 cut(s)	480, 806, 945
ErhI	CCWWGG	1 cut(s)	702
Esp3I	CGTCTC	1 cut(s)	383
FaeI	CATG	4 cut(s)	434, 589, 706, 938
FaqI	GGGAC	4 cut(s)	712, 730, 1220, 1317
FatI	CATG	4 cut(s)	430, 585, 702, 934
FauI	CCCGC	1 cut(s)	267
FblI	GTMKAC	1 cut(s)	1380
Fnu4HI	GCNGC	2 cut(s)	1008, 1179
FokI	GGATG	3 cut(s)	128, 932, 1005
FriOI	GRGCYC	3 cut(s)	480, 806, 945
Fsp4HI	GCNGC	2 cut(s)	1008, 1179
FspBI	CTAG	1 cut(s)	1245
GlaI	GCGC	2 cut(s)	238, 374
GluI	GCNGC	2 cut(s)	1008, 1179
GsaI	CCCAGC	1 cut(s)	1115
GsuI	CTGGAG	1 cut(s)	1218
HaeII	RGCGCY	1 cut(s)	240
HaeIII	GGCC	3 cut(s)	314, 1121, 1427
HapII	CCGG	2 cut(s)	991, 1122
HgaI	GACGC	1 cut(s)	1366
HhaI	GCGC	2 cut(s)	239, 375
Hin1II	CATG	4 cut(s)	434, 589, 706, 938
Hin6I	GCGC	2 cut(s)	237, 373
HinP1I	GCGC	2 cut(s)	237, 373
HincII	GTYRAC	1 cut(s)	1381
HindII	GTYRAC	1 cut(s)	1381
HinfI	GANTC	6 cut(s)	412, 542, 718, 833, 1328, 1340
HpaII	CCGG	2 cut(s)	991, 1122
HphI	GGTGA	4 cut(s)	289, 317, 500, 1136
Hpy166II	GTNNAC	2 cut(s)	829, 1381
Hpy188I	TCNGA	7 cut(s)	114, 517, 642, 671, 1050, 1211, 1327
Hpy188III	TCNNGA	6 cut(s)	600, 688, 697, 790, 830, 1064
Hpy8I	GTNNAC	2 cut(s)	829, 1381
Hpy99I	CGWCG	1 cut(s)	1382
HpyAV	CCTTC	6 cut(s)	105, 332, 532, 796, 859, 1328
HpyCH4III	ACNGT	5 cut(s)	181, 305, 1030, 1081, 1441
HpyCH4IV	ACGT	1 cut(s)	627
HpyF10VI	GCNNNNNNNGC	4 cut(s)	372, 940, 1112, 1396
HpyF3I	CTNAG	2 cut(s)	1152, 1257
HpySE526I	ACGT	1 cut(s)	627
Hsp92II	CATG	4 cut(s)	434, 589, 706, 938
HspAI	GCGC	2 cut(s)	237, 373
Kzo9I	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
LguI	GCTCTTC	1 cut(s)	933
LmnI	GCTCC	2 cut(s)	257, 1261
Lsp1109I	GCAGC	1 cut(s)	994
LweI	GCATC	1 cut(s)	921
MaeI	CTAG	1 cut(s)	1245
MaeII	ACGT	1 cut(s)	627
MaeIII	GTNAC	3 cut(s)	159, 1186, 1441
MalI	GATC	7 cut(s)	74, 86, 140, 435, 594, 885, 1374
MboI	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
MboII	GAAGA	3 cut(s)	950, 1129, 1447
MhlI	GDGCHC	4 cut(s)	480, 806, 945, 1266
MluCI	AATT	7 cut(s)	35, 753, 1095, 1145, 1212, 1298, 1351
MlyI	GAGTC	3 cut(s)	406, 712, 1322
MmeI	TCCRAC	2 cut(s)	100, 428
Mph1103I	ATGCAT	1 cut(s)	936
MseI	TTAA	2 cut(s)	104, 986
MspA1I	CMGCKG	2 cut(s)	274, 568
MspI	CCGG	2 cut(s)	991, 1122
MspR9I	CCNGG	1 cut(s)	1054
MvaI	CCWGG	1 cut(s)	1054
MvnI	CGCG	1 cut(s)	1377
MwoI	GCNNNNNNNGC	4 cut(s)	372, 940, 1112, 1396
NcoI	CCATGG	1 cut(s)	702
NdeII	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
NlaIII	CATG	4 cut(s)	434, 589, 706, 938
NlaIV	GGNNCC	3 cut(s)	406, 700, 1426
NmuCI	GTSAC	2 cut(s)	159, 1441
NsiI	ATGCAT	1 cut(s)	936
NspV	TTCGAA	1 cut(s)	836
PciSI	GCTCTTC	1 cut(s)	933
PfeI	GAWTC	3 cut(s)	542, 833, 1340
PinAI	ACCGGT	1 cut(s)	990
PkrI	GCNGC	2 cut(s)	1009, 1180
Ple19I	CGATCG	1 cut(s)	87
PleI	GAGTC	3 cut(s)	406, 712, 1322
PpsI	GAGTC	3 cut(s)	406, 712, 1322
Psp124BI	GAGCTC	3 cut(s)	480, 806, 945
Psp6I	CCWGG	1 cut(s)	1052
PspFI	CCCAGC	1 cut(s)	1111
PspGI	CCWGG	1 cut(s)	1052
PspN4I	GGNNCC	3 cut(s)	406, 700, 1426
PspPI	GGNCC	4 cut(s)	312, 597, 699, 1425
PvuI	CGATCG	1 cut(s)	87
RsaI	GTAC	2 cut(s)	172, 1367
RsaNI	GTAC	2 cut(s)	171, 1366
SacI	GAGCTC	3 cut(s)	480, 806, 945
SalI	GTCGAC	1 cut(s)	1379
SapI	GCTCTTC	1 cut(s)	933
SaqAI	TTAA	2 cut(s)	104, 986
SatI	GCNGC	2 cut(s)	1008, 1179
Sau3AI	GATC	7 cut(s)	72, 84, 138, 433, 592, 883, 1372
Sau96I	GGNCC	4 cut(s)	312, 597, 699, 1425
SchI	GAGTC	3 cut(s)	406, 712, 1322
ScrFI	CCNGG	1 cut(s)	1054
SduI	GDGCHC	4 cut(s)	480, 806, 945, 1266
SfaNI	GCATC	1 cut(s)	921
SfuI	TTCGAA	1 cut(s)	836
SinI	GGWCC	2 cut(s)	597, 699
Sse9I	AATT	7 cut(s)	35, 753, 1095, 1145, 1212, 1298, 1351
SsiI	CCGC	5 cut(s)	255, 274, 568, 1179, 1313
SspI	AATATT	1 cut(s)	727
SspMI	CTAG	1 cut(s)	1245
SstI	GAGCTC	3 cut(s)	480, 806, 945
StyD4I	CCNGG	1 cut(s)	1052
StyI	CCWWGG	1 cut(s)	702
TaaI	ACNGT	5 cut(s)	181, 305, 1030, 1081, 1441
TaiI	ACGT	1 cut(s)	630
TaqI	TCGA	4 cut(s)	83, 504, 836, 1380
TaqII	GACCGA	3 cut(s)	386, 585, 1004
TasI	AATT	7 cut(s)	35, 753, 1095, 1145, 1212, 1298, 1351
TauI	GCSGC	1 cut(s)	1181
TfiI	GAWTC	3 cut(s)	542, 833, 1340
Tru1I	TTAA	2 cut(s)	104, 986
Tru9I	TTAA	2 cut(s)	104, 986
TscAI	CASTG	4 cut(s)	466, 886, 1091, 1408
TseFI	GTSAC	2 cut(s)	159, 1441
TseI	GCWGC	1 cut(s)	1007
Tsp45I	GTSAC	2 cut(s)	159, 1441
TspDTI	ATGAA	6 cut(s)	237, 542, 736, 936, 951, 1332
TspGWI	ACGGA	4 cut(s)	178, 431, 932, 1153
TspRI	CASTG	4 cut(s)	466, 886, 1091, 1408
VpaK11BI	GGWCC	2 cut(s)	597, 699
XapI	RAATTY	1 cut(s)	35
XcmI	CCANNNNNNNNNTGG	1 cut(s)	398
XmiI	GTMKAC	1 cut(s)	1380
XspI	CTAG	1 cut(s)	1245
Zsp2I	ATGCAT	1 cut(s)	936

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.