MD15G1061100.v1.1

Putative Phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016768
Physical Location & Seq
Forward (+)
4105657 .. 4107513
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1061100.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCGGCGGGGATGGTGGTTGTTTTCGACTTTGACAAGACGATCATCGACTGCGACAGCGACAACTGGGTCGTGGACGAGTTGGGTGTCGATGACTTGTTCACTCAGCTCCTCCCTACCATGCCTTGGAACCCTCTTATGGATCGGATGATGACGGCGCTTCATGCACGCAGAAAGACAATTCAGGACATTTCGGAGTGCTTAAAGAGGGTTCCAATCCATCCCAACATTGTCTCTGCAATTAAATCAGCCCATGCTTTTGGGTGTGATCTGAGGGTTTTAAGTGATGCAAATGATTTTTTCATAGACGCAATATTGAAGCATCATGGGATAATGGATTGCTTCTCAGAAATCAAGACAAACCCTAGCTTTATTGATGAACAACGCAGGCTGAGAATCTTGCCCTTCCATGATTTCATCTCGTCTTCCCATGGCTGCACTCTTTGCCCTCCCAACATGTGCAAGGGTTTAGTGATGGAAAAGATCCGAGCTTCTGTTTCAGCAGATGGGAAGAAGCATTTCATCTATGTTGGAGATGGAGCTCCCGATTTTTGCGCGGGTTTGAAGCTTGAAGAAGGGGACTTTCTGTTGCCGAGGAAGAACTATCCAATCTGGGATTTGATTTCTGCCAATCCCTTGCTTACCAAGGCAAAGATTCACGAGTGGAACGAGTGCGATGAGCTCGGGACTATTTTGCTCAACACAGTGAAGGCATTCTTCATTGGAGACAAGTCTGGCAAAGAGAGAAGTAATCGTTTGGTTCCAATTGATTGCAAGTCTCAAAGCAGTTCGGTATCTGCCTCTGCCCATGATGCCTTTCAAAATGCCCTCCCAGTTCCGCCTCCTCGCCAGTAA

Protein Analysis

285

Amino Acids

31.74

Weight (kDa)

5.92

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Put_Phosphatase PF06888 5 - 235 4.6e-87 Putative Phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016768)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32070
malus_domestica MD15G1061100.v1.1
prunus_persica Prupe.1G413800_v2.0.a1
pyrus_communis pycom15g05720
rosa_chinensis RchiOBHm_Chr6g0310671
rosa_laevigata RLG00000010467
rosa_multiflora Rmu_sc0002546.1_g000002
rosa_roxburghii Rroxscaffold_7G00158360
rosa_rugosa Rorug06G0382100
rosa_samantha Rh6AG496100 Rh6BG505600 Rh6CG511300 Rh6DG497000
rosa_wichuraiana Rw6G043140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 68
AccB7I CCANNNNNTGG 1 cut(s) 126
AccII CGCG 1 cut(s) 557
AciI CCGC 4 cut(s) 5, 8, 557, 839
AclWI GGATC 2 cut(s) 150, 478
AfiI CCNNNNNNNGG 2 cut(s) 126, 139
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 4 cut(s) 319, 565, 572, 821
AluBI AGCT 6 cut(s) 109, 369, 491, 542, 568, 682
AluI AGCT 6 cut(s) 109, 369, 491, 542, 568, 682
Alw21I GWGCWC 2 cut(s) 544, 684
Alw26I GTCTC 3 cut(s) 238, 720, 783
AlwI GGATC 2 cut(s) 150, 478
Ama87I CYCGRG 1 cut(s) 683
ApeKI GCWGC 1 cut(s) 435
Asp700I GAANNNNTTC 2 cut(s) 518, 713
AspLEI GCGC 2 cut(s) 160, 557
AvaI CYCGRG 1 cut(s) 683
BanII GRGCYC 2 cut(s) 544, 684
BauI CACGAG 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 2 cut(s) 544, 684
BbvI GCAGC 1 cut(s) 422
BccI CCATC 5 cut(s) 7, 228, 469, 500, 530
BceAI ACGGC 1 cut(s) 171
BcoDI GTCTC 3 cut(s) 238, 720, 783
BfaI CTAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 161
BisI GCNGC 2 cut(s) 6, 436
BlsI GCNGC 2 cut(s) 7, 437
BmeT110I CYCGRG 1 cut(s) 683
BmiI GGNNCC 3 cut(s) 131, 213, 762
BmrI ACTGGG 2 cut(s) 76, 827
BmsI GCATC 3 cut(s) 277, 331, 802
BmuI ACTGGG 2 cut(s) 76, 827
BpiI GAAGAC 1 cut(s) 417
BsaJI CCNNGG 4 cut(s) 125, 430, 593, 645
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 139
Bse1I ACTGG 3 cut(s) 71, 833, 850
BseDI CCNNGG 4 cut(s) 125, 430, 593, 645
BseGI GGATG 3 cut(s) 18, 153, 220
BseLI CCNNNNNNNGG 2 cut(s) 126, 139
BseMII CTCAG 4 cut(s) 119, 263, 360, 383
BseNI ACTGG 3 cut(s) 71, 833, 850
BseRI GAGGAG 2 cut(s) 101, 834
BseXI GCAGC 1 cut(s) 422
BsgI GTGCAG 1 cut(s) 421
Bsh1236I CGCG 1 cut(s) 557
BsiHKAI GWGCWC 2 cut(s) 544, 684
BsiHKCI CYCGRG 1 cut(s) 683
BslFI GGGAC 2 cut(s) 593, 700
BslI CCNNNNNNNGG 2 cut(s) 126, 139
BsmAI GTCTC 3 cut(s) 238, 720, 783
BsmFI GGGAC 2 cut(s) 593, 700
BsmI GAATGC 1 cut(s) 713
BsoBI CYCGRG 1 cut(s) 683
Bsp1286I GDGCHC 2 cut(s) 544, 684
Bsp143I GATC 4 cut(s) 42, 142, 268, 483
Bsp19I CCATGG 1 cut(s) 430
BspACI CCGC 4 cut(s) 5, 8, 557, 839
BspCNI CTCAG 4 cut(s) 118, 264, 359, 384
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 3 cut(s) 131, 213, 762
BspPI GGATC 2 cut(s) 150, 478
BsrI ACTGG 3 cut(s) 71, 833, 850
BssECI CCNNGG 4 cut(s) 125, 430, 593, 645
BssMI GATC 4 cut(s) 42, 142, 268, 483
BssSI CACGAG 1 cut(s) 659
BssT1I CCWWGG 3 cut(s) 125, 430, 645
Bst2BI CACGAG 1 cut(s) 659
Bst4CI ACNGT 1 cut(s) 706
BstAPI GCANNNNNTGC 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 169, 389
BstDEI CTNAG 4 cut(s) 105, 272, 346, 392
BstDSI CCRYGG 1 cut(s) 430
BstF5I GGATG 3 cut(s) 18, 153, 220
BstFNI CGCG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 2 cut(s) 160, 557
BstKTI GATC 4 cut(s) 45, 145, 271, 486
BstMAI GTCTC 3 cut(s) 238, 720, 783
BstMBI GATC 4 cut(s) 42, 142, 268, 483
BstMWI GCNNNNNNNGC 3 cut(s) 164, 444, 812
BstNSI RCATGY 1 cut(s) 460
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 1 cut(s) 422
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 483
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 430
BtgZI GCGATG 1 cut(s) 690
BtsCI GGATG 3 cut(s) 18, 153, 220
BtsIMutI CAGTG 1 cut(s) 711
Cac8I GCNNGC 2 cut(s) 169, 389
CfoI GCGC 2 cut(s) 160, 557
CseI GACGC 1 cut(s) 317
CviAII CATG 8 cut(s) 121, 164, 254, 326, 410, 431, 457, 809
CviJI RGCY 9 cut(s) 109, 251, 369, 391, 435, 491, 542, 568, 682
CviKI_1 RGCY 9 cut(s) 109, 251, 369, 391, 435, 491, 542, 568, 682
DdeI CTNAG 4 cut(s) 105, 272, 346, 392
DpnI GATC 4 cut(s) 44, 144, 270, 485
DpnII GATC 4 cut(s) 42, 142, 268, 483
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 828
Ecl136II GAGCTC 2 cut(s) 542, 682
Eco130I CCWWGG 3 cut(s) 125, 430, 645
Eco24I GRGCYC 2 cut(s) 544, 684
Eco53kI GAGCTC 2 cut(s) 542, 682
Eco88I CYCGRG 1 cut(s) 683
EcoICRI GAGCTC 2 cut(s) 542, 682
EcoT14I CCWWGG 3 cut(s) 125, 430, 645
EcoT38I GRGCYC 2 cut(s) 544, 684
ErhI CCWWGG 3 cut(s) 125, 430, 645
FaeI CATG 8 cut(s) 124, 167, 257, 329, 413, 434, 460, 812
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 2 cut(s) 593, 700
FatI CATG 8 cut(s) 120, 163, 253, 325, 409, 430, 456, 808
FauI CCCGC 1 cut(s) 550
Fnu4HI GCNGC 2 cut(s) 6, 436
FokI GGATG 3 cut(s) 25, 160, 207
FriOI GRGCYC 2 cut(s) 544, 684
Fsp4HI GCNGC 2 cut(s) 6, 436
FspBI CTAG 1 cut(s) 366
GlaI GCGC 2 cut(s) 159, 556
GluI GCNGC 2 cut(s) 6, 436
HaeII RGCGCY 1 cut(s) 161
HgaI GACGC 1 cut(s) 317
HhaI GCGC 2 cut(s) 160, 557
Hin1II CATG 8 cut(s) 124, 167, 257, 329, 413, 434, 460, 812
Hin6I GCGC 2 cut(s) 158, 555
HinP1I GCGC 2 cut(s) 158, 555
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 2 cut(s) 396, 655
Hpy166II GTNNAC 2 cut(s) 76, 102
Hpy188I TCNGA 5 cut(s) 147, 196, 273, 349, 488
Hpy188III TCNNGA 5 cut(s) 185, 355, 545, 659, 685
Hpy8I GTNNAC 2 cut(s) 76, 102
HpyAV CCTTC 3 cut(s) 415, 569, 703
HpyCH4III ACNGT 1 cut(s) 706
HpyCH4V TGCA 6 cut(s) 167, 239, 290, 438, 462, 774
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 444, 812
HpyF3I CTNAG 4 cut(s) 105, 272, 346, 392
Hsp92II CATG 8 cut(s) 124, 167, 257, 329, 413, 434, 460, 812
HspAI GCGC 2 cut(s) 158, 555
Kzo9I GATC 4 cut(s) 42, 142, 268, 483
LmnI GCTCC 3 cut(s) 114, 539, 547
LpnPI CCDG 6 cut(s) 52, 170, 373, 598, 720, 846
Lsp1109I GCAGC 1 cut(s) 422
LweI GCATC 3 cut(s) 277, 331, 802
MaeI CTAG 1 cut(s) 366
MalI GATC 4 cut(s) 44, 144, 270, 485
MboI GATC 4 cut(s) 42, 142, 268, 483
MboII GAAGA 5 cut(s) 417, 523, 584, 610, 709
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 1 cut(s) 483
MhlI GDGCHC 2 cut(s) 544, 684
MluCI AATT 3 cut(s) 180, 240, 765
MmeI TCCRAC 1 cut(s) 511
MroXI GAANNNNTTC 2 cut(s) 518, 713
MseI TTAA 3 cut(s) 203, 243, 281
MunI CAATTG 1 cut(s) 765
Mva1269I GAATGC 1 cut(s) 713
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 3 cut(s) 164, 444, 812
NcoI CCATGG 1 cut(s) 430
NdeII GATC 4 cut(s) 42, 142, 268, 483
NlaIII CATG 8 cut(s) 124, 167, 257, 329, 413, 434, 460, 812
NlaIV GGNNCC 3 cut(s) 131, 213, 762
NmeAIII GCCGAG 1 cut(s) 618
NspI RCATGY 1 cut(s) 460
PciI ACATGT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 666
PctI GAATGC 1 cut(s) 713
PdmI GAANNNNTTC 2 cut(s) 518, 713
PfeI GAWTC 2 cut(s) 396, 655
PflMI CCANNNNNTGG 1 cut(s) 126
PkrI GCNGC 2 cut(s) 7, 437
PscI ACATGT 1 cut(s) 456
Psp124BI GAGCTC 2 cut(s) 544, 684
PspN4I GGNNCC 3 cut(s) 131, 213, 762
PsuI RGATCY 1 cut(s) 483
SacI GAGCTC 2 cut(s) 544, 684
SaqAI TTAA 3 cut(s) 203, 243, 281
SatI GCNGC 2 cut(s) 6, 436
Sau3AI GATC 4 cut(s) 42, 142, 268, 483
SduI GDGCHC 2 cut(s) 544, 684
SetI ASST 6 cut(s) 111, 371, 493, 544, 570, 684
SfaNI GCATC 3 cut(s) 277, 331, 802
Sse9I AATT 3 cut(s) 180, 240, 765
SsiI CCGC 4 cut(s) 5, 8, 557, 839
SspI AATATT 1 cut(s) 315
SspMI CTAG 1 cut(s) 366
SstI GAGCTC 2 cut(s) 544, 684
StyI CCWWGG 3 cut(s) 125, 430, 645
TaaI ACNGT 1 cut(s) 706
TaqI TCGA 3 cut(s) 27, 48, 90
TasI AATT 3 cut(s) 180, 240, 765
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 396, 655
Tru1I TTAA 3 cut(s) 203, 243, 281
Tru9I TTAA 3 cut(s) 203, 243, 281
TscAI CASTG 1 cut(s) 711
TseI GCWGC 1 cut(s) 435
TspDTI ATGAA 6 cut(s) 152, 292, 393, 406, 511, 709
TspRI CASTG 1 cut(s) 711
Van91I CCANNNNNTGG 1 cut(s) 126
XceI RCATGY 1 cut(s) 460
XmnI GAANNNNTTC 2 cut(s) 518, 713
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.