Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011338

Physical Location & Seq

Genomic Coordinates Forward (+)

5607662 .. 5611402

Gene Structure 3741 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1081700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGGAAGACGATGAGATAGAGGAGGGGATGCTGGTGGAGGAAGACGCCGCTCCGCCGCCGCCGAAGAAGCCGGGAAAATCCCCGTACGAAATGCTTCAGGAGAGCAAGTCCTCAGTTGAGGACATAGTGACGAAGATGCTCACCATCAAAAAAGAAGCCAAACCTAAATCGGAGCTCCGCGAGCTCGTCACTCAGATGTTCCTCAACTTCGTCACCCTCCGCCAGGCGAATCGTTCGATCTTGCTCGAGGAGGACCGCGTGAAGGCGGGGACGGAGAGTGCGAAGGCTCCGGTGGACTTGACGACGCTGCAGCTCCACAATCTGATGTACGAGAAGAGCCACTACGTGAAGGCCATTAAAGCTTGCAAGGACTTCAAGTCTAAGTACCCGGACATCGAGCTGGTCCCGGAAGAAGAGTTCTTTCGCGACGCCCCGGCCGATATCAAGGAGCCGACGCTGTCGAATGATGCAGGGCATGACATTATGCTCAAGAGGCTCAATTTTGAGCTCCACCAGCGCAAGGAGCTCTGCAAGCATCATGAGAAGCTTGAGATTCATAAGAAGGGTCTGTTGGAGATGATTGCCAATCGGAAGAAGTTCTTGTCCAGTCTTCCTTCGCACTTAAAGTCGCTAAAGAAGGCTTCTTTGCCGGTGCAGAACCAATTGGGGCTTCAGCATACCAAGAAGCTCAAGCAGCATCACGCTGCAGAGTTGCTTCCACCGCCTCTGTATGTAGTTTACTCGCAGTTCATGGCGCAGAAGGAGGCATTTGATGAACAGATTGAGTTGGATATAGTGGGGAGTGTGAAGGACGCTCAAGCTTTTGCCCATAAGCAAGCAAATAAGGAGCCTGGAGTGTCTACCAGTGTAGAGACTAGTAGGGATGATGATGCACTGGATGAGGAAGATGATGGGCAGAGAAGGAGAAAGCGGCCAAAGAGGGCTCCGGTCAAGCAAAACCTTGAACAGTCAGGACTGTATCAAGTTCATCCGCTGAAAATCATCCTTCATATTTATGATGATGAGGTTTCTGATCCCAAGTCGGCAAAACTTATCACCCTCAAGTTTGAGTTTTTGTTGAAGTTGAATGTTGTATGTGTTGGAATTGAAGGATCCAATGATGCAGCGGAAAATAGCACCTTATGCAACCTATTCCCAGATGACACTGGCCTTGAGCTTCCCCATCAGTCAGCCAAGCTCGTTATGGGTGATACTCTTGCATTTGATGAAAAGAGAACTTCCCGTCCATATAAGTGGGCCCAGCATTTGGCGGGAATTGATTTCTTGCCTGAGGTGGCACCTTTGCTTGCTGCCCCTGAAACTCCAAGTTGTGATACTGCTAAAAGTGATGCTGTTATATCTGGTCTGTCACTTTACCGCCACCAGAACCGAATACAGACAGTTGTGCGAAGAATTCGCTCTCGGAGAAAAGCTCAGATGGCTCTTGTGGAACAGATCGAATCACTTATGAAGCTTAAATGGCCGTCTGTGTCTTGTGAAAGTGTTCCATGGGCTTTGCATACCCCTTTGTGCAAATTGCATGGTTTTTCACCTGTTGGGCCTCCACCTAATGTGGCATCATCTTTGTCTGTCCTAGATAAGGAGCAGGGTCAGGAGCCTATGGATGTTGATTTGGTTGGACGATCTGGTTCTTCAAAGGAAGAGCTGGAGAGTATCAGAGAAGATGGGGAACTTCCATCTTTAGTTCCAGCTGCATCCATTGCCAATGATAGTAAACTTGCTCACCATAAAGGAGCTATTCTTGACCACCCCAGACGGCCGTCCTTAGTTCCAAAAAGACCTCCAATCAGCAAGGCCAAATCGCTAAGTTATAAGAAACTTGATGAAGATTTAGATCTTCTTCTGGATACAGAGAGTGATCAGGACGAACCTGCACATGTGCTAGAGGAAGAAAATTTAGCGTATGTTGAATGCTTTGGGGTGGCTGGAACGTCATGGGTGGATTTTGGGGTCAGAGAATATTGTCTTGTTTTAACCAGGAGAGTTGACAAGGATAAGAGGAATGTGAAGTTAGAAGCCAAGATTAAGATCAGCATGGAGTATCCATTAAGGCCTCCCTATTTTGCACTCAGTCTTAGCACTATATCTGGAGAAAACCATAAAGAGAGCGATGACTCTGAGTGCTTCAATGAACTTCGTGCAATGGAAGCAGAGGTCAATCTGCACATGGTGAAGATGTTACCTCAAACTCAAAGTGAAGAAAATAATATCTTAGCTCATCAGGTTTGTTGTCTGTCAATGTTATTCGACTATTACATGGACGAAGCATCCCCATCTTCTGAAAAGAGACGGAGCACTTCTGTTGTTGATGTTGGTTTGTGCAAGCCCATCAGCGGTCAGATTGTTGCCAGATCATTTAGAGGAAGGGATCGCAGGAAGATGATATCCTGGAAGGACATGGAATGCACTTCAGGCTATCCTTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000346 GO:0000347 GO:0000445 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006355 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015031 GO:0015833 GO:0015931 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031503 GO:0031974 GO:0031981 GO:0032784 GO:0032786 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0045893 GO:0045935 GO:0046907 GO:0048518 GO:0048522 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0097159 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

FimP

Protein Analysis

816

Amino Acids

91.97

Weight (kDa)

6.01

Isoelectric Point (pI)

57.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FmiP_Thoc5	PF09766	72 - 428	7.7e-115	Fms-interacting protein/Thoc5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011338)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45233 AT5G42920 AT5G42920
fragaria_vesca	FvH4_2g34320 FvH4_2g34320
malus_domestica	MD15G1081700.v1.1
prunus_persica	Prupe.1G434400_v2.0.a1
pyrus_communis	pycom08g08080 pycom15g07640
rosa_chinensis	RchiOBHm_Chr6g0308041
rosa_laevigata	RLG00000010673
rosa_multiflora	Rmu_co8496519.1_g000001 Rmu_sc0002624.1_g000002
rosa_roxburghii	Rroxscaffold_7G00160640
rosa_rugosa	Rorug06G0363300
rosa_samantha	Rh6AG472200 Rh6BG483200 Rh6CG488300 Rh6DG474600
rosa_wichuraiana	Rw6G041210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1833
Acc36I	ACCTGC	1 cut(s)	1900
AccB1I	GGYRCC	1 cut(s)	1297
AccB7I	CCANNNNNTGG	1 cut(s)	1267
AccBSI	CCGCTC	1 cut(s)	50
AccI	GTMKAC	1 cut(s)	860
AccII	CGCG	3 cut(s)	180, 258, 426
AclWI	GGATC	4 cut(s)	1028, 1107, 1120, 2397
AcoI	YGGCCR	4 cut(s)	435, 932, 1481, 1778
AcsI	RAATTY	2 cut(s)	1413, 1915
AcuI	CTGAAG	3 cut(s)	80, 656, 2415
AcyI	GRCGYC	2 cut(s)	45, 429
AdeI	CACNNNGTG	2 cut(s)	346, 2191
AfaI	GTAC	3 cut(s)	86, 329, 386
AfiI	CCNNNNNNNGG	6 cut(s)	223, 262, 520, 1267, 1600, 2354
AflIII	ACRYGT	1 cut(s)	1897
AgsI	TTSAA	8 cut(s)	376, 965, 1081, 1087, 1109, 1656, 1931, 2149
AhdI	GACNNNNNGTC	1 cut(s)	376
AhlI	ACTAGT	1 cut(s)	875
AjnI	CCWGG	4 cut(s)	222, 850, 1997, 2408
AjuI	GAANNNNNNNTTGG	4 cut(s)	554, 586, 654, 686
AloI	GAACNNNNNNTCC	4 cut(s)	401, 433, 1228, 1260
Alw21I	GWGCWC	5 cut(s)	177, 186, 510, 528, 2318
Alw26I	GTCTC	2 cut(s)	866, 2302
AlwI	GGATC	4 cut(s)	1028, 1107, 1120, 2397
Ama87I	CYCGRG	1 cut(s)	245
ApaI	GGGCCC	1 cut(s)	1261
ApeKI	GCWGC	7 cut(s)	307, 310, 694, 704, 1124, 1310, 1712
ApoI	RAATTY	2 cut(s)	1413, 1915
Asp700I	GAANNNNTTC	3 cut(s)	93, 596, 1503
AspLEI	GCGC	2 cut(s)	519, 757
AspS9I	GGNCC	5 cut(s)	253, 403, 1257, 1258, 1559
AsuC2I	CCSGG	4 cut(s)	72, 389, 407, 434
AsuHPI	GGTGA	7 cut(s)	133, 205, 1048, 1220, 1542, 1736, 2203
AvaI	CYCGRG	1 cut(s)	245
AvaII	GGWCC	2 cut(s)	253, 403
AxyI	CCTNAGG	1 cut(s)	1290
BaeGI	GKGCMC	1 cut(s)	1261
BamHI	GGATCC	1 cut(s)	1112
BanI	GGYRCC	1 cut(s)	1297
BanII	GRGCYC	6 cut(s)	177, 186, 510, 528, 946, 1261
BarI	GAAGNNNNNNTAC	2 cut(s)	326, 358
BbsI	GAAGAC	3 cut(s)	12, 48, 602
Bbv12I	GWGCWC	5 cut(s)	177, 186, 510, 528, 2318
BbvI	GCAGC	7 cut(s)	294, 322, 691, 706, 1136, 1297, 1699
BccI	CCATC	8 cut(s)	152, 905, 1191, 1432, 1679, 1705, 2302, 2357
BceAI	ACGGC	3 cut(s)	1468, 1765, 1793
BciT130I	CCWGG	4 cut(s)	224, 852, 1999, 2410
BciVI	GTATCC	2 cut(s)	1861, 2073
BclI	TGATCA	1 cut(s)	1879
BcnI	CCSGG	4 cut(s)	72, 389, 407, 434
BcoDI	GTCTC	2 cut(s)	866, 2302
BcuI	ACTAGT	1 cut(s)	875
BfaI	CTAG	4 cut(s)	876, 1595, 1904, 2446
BfmI	CTRYAG	2 cut(s)	308, 705
BfuAI	ACCTGC	1 cut(s)	1900
BfuI	GTATCC	2 cut(s)	1861, 2073
BglII	AGATCT	1 cut(s)	1855
Bme1390I	CCNGG	8 cut(s)	72, 224, 389, 407, 434, 852, 1999, 2410
Bme18I	GGWCC	2 cut(s)	253, 403
BmeRI	GACNNNNNGTC	1 cut(s)	376
BmeT110I	CYCGRG	1 cut(s)	245
BmgT120I	GGNCC	5 cut(s)	253, 403, 1257, 1258, 1559
BmiI	GGNNCC	9 cut(s)	288, 405, 450, 849, 945, 1114, 1259, 1299, 1617
BmrFI	CCNGG	8 cut(s)	72, 224, 389, 407, 434, 852, 1999, 2410
BoxI	GACNNNNGTC	1 cut(s)	1780
BpiI	GAAGAC	3 cut(s)	12, 48, 602
BplI	GAGNNNNNCTC	2 cut(s)	1417, 1449
BpmI	CTGGAG	3 cut(s)	873, 1688, 2130
BpuEI	CTTGAG	6 cut(s)	473, 569, 674, 801, 1046, 1193
BpuMI	CCSGG	4 cut(s)	72, 389, 407, 434
BsaAI	YACGTR	1 cut(s)	346
BsaBI	GATNNNNATC	2 cut(s)	1854, 2048
BsaHI	GRCGYC	2 cut(s)	45, 429
BsaJI	CCNNGG	2 cut(s)	432, 1508
BsaWI	WCCGGW	2 cut(s)	289, 946
BsaXI	ACNNNNNCTCC	2 cut(s)	92, 122
Bsc4I	CCNNNNNNNGG	6 cut(s)	223, 262, 520, 1267, 1600, 2354
Bse118I	RCCGGY	1 cut(s)	649
Bse1I	ACTGG	4 cut(s)	606, 864, 900, 1171
Bse21I	CCTNAGG	1 cut(s)	1290
Bse3DI	GCAATG	2 cut(s)	1719, 2169
Bse8I	GATNNNNATC	2 cut(s)	1854, 2048
BseBI	CCWGG	4 cut(s)	224, 852, 1999, 2410
BseDI	CCNNGG	2 cut(s)	432, 1508
BseGI	GGATG	8 cut(s)	33, 889, 904, 988, 1002, 1630, 1715, 2288
BseJI	GATNNNNATC	2 cut(s)	1854, 2048
BseLI	CCNNNNNNNGG	6 cut(s)	223, 262, 520, 1267, 1600, 2354
BseMI	GCAATG	2 cut(s)	1719, 2169
BseMII	CTCAG	6 cut(s)	126, 206, 1281, 1448, 2104, 2130
BseNI	ACTGG	4 cut(s)	606, 864, 900, 1171
BseRI	GAGGAG	2 cut(s)	35, 263
BseSI	GKGCMC	1 cut(s)	1261
BseX3I	CGGCCG	2 cut(s)	435, 1778
BseXI	GCAGC	7 cut(s)	294, 322, 691, 706, 1136, 1297, 1699
BseYI	CCCAGC	1 cut(s)	1260
BsgI	GTGCAG	3 cut(s)	674, 1878, 2168
Bsh1236I	CGCG	3 cut(s)	180, 258, 426
Bsh1285I	CGRYCG	2 cut(s)	438, 1781
BshNI	GGYRCC	1 cut(s)	1297
BsiEI	CGRYCG	2 cut(s)	438, 1781
BsiHKAI	GWGCWC	5 cut(s)	177, 186, 510, 528, 2318
BsiHKCI	CYCGRG	1 cut(s)	245
BsiSI	CCGG	7 cut(s)	71, 290, 389, 407, 434, 650, 947
BsiWI	CGTACG	1 cut(s)	84
BslFI	GGGAC	2 cut(s)	283, 389
BslI	CCNNNNNNNGG	6 cut(s)	223, 262, 520, 1267, 1600, 2354
BsmAI	GTCTC	2 cut(s)	866, 2302
BsmBI	CGTCTC	1 cut(s)	2302
BsmFI	GGGAC	2 cut(s)	283, 389
BsmI	GAATGC	2 cut(s)	1937, 2429
BsoBI	CYCGRG	1 cut(s)	245
Bsp120I	GGGCCC	1 cut(s)	1257
Bsp1286I	GDGCHC	7 cut(s)	177, 186, 510, 528, 946, 1261, 2318
Bsp19I	CCATGG	1 cut(s)	1508
Bsp68I	TCGCGA	1 cut(s)	426
BspCNI	CTCAG	6 cut(s)	125, 205, 1282, 1447, 2103, 2131
BspFNI	CGCG	3 cut(s)	180, 258, 426
BspHI	TCATGA	1 cut(s)	538
BspLI	GGNNCC	9 cut(s)	288, 405, 450, 849, 945, 1114, 1259, 1299, 1617
BspMAI	CTGCAG	2 cut(s)	312, 709
BspMI	ACCTGC	1 cut(s)	1900
BspPI	GGATC	4 cut(s)	1028, 1107, 1120, 2397
BspQI	GCTCTTC	2 cut(s)	329, 1656
BspT107I	GGYRCC	1 cut(s)	1297
BsrBI	CCGCTC	1 cut(s)	50
BsrDI	GCAATG	2 cut(s)	1719, 2169
BsrFI	RCCGGY	1 cut(s)	649
BsrI	ACTGG	4 cut(s)	606, 864, 900, 1171
BssAI	RCCGGY	1 cut(s)	649
BssECI	CCNNGG	2 cut(s)	432, 1508
BssNI	GRCGYC	2 cut(s)	45, 429
BssT1I	CCWWGG	1 cut(s)	1508
Bst2UI	CCWGG	4 cut(s)	224, 852, 1999, 2410
Bst4CI	ACNGT	3 cut(s)	969, 978, 1402
Bst6I	CTCTTC	3 cut(s)	329, 408, 1656
BstACI	GRCGYC	2 cut(s)	45, 429
BstAPI	GCANNNNNTGC	2 cut(s)	1143, 1721
BstBAI	YACGTR	1 cut(s)	346
BstC8I	GCNNGC	6 cut(s)	182, 364, 533, 837, 1308, 2345
BstDSI	CCRYGG	1 cut(s)	1508
BstENI	CCTNNNNNAGG	2 cut(s)	221, 1598
BstF5I	GGATG	8 cut(s)	33, 889, 904, 988, 1002, 1630, 1715, 2288
BstFNI	CGCG	3 cut(s)	180, 258, 426
BstHHI	GCGC	2 cut(s)	519, 757
BstMAI	GTCTC	2 cut(s)	866, 2302
BstMCI	CGRYCG	2 cut(s)	438, 1781
BstNI	CCWGG	4 cut(s)	224, 852, 1999, 2410
BstNSI	RCATGY	1 cut(s)	1901
BstPAI	GACNNNNGTC	1 cut(s)	1780
BstSCI	CCNGG	8 cut(s)	70, 222, 387, 405, 432, 850, 1997, 2408
BstSFI	CTRYAG	2 cut(s)	308, 705
BstSLI	GKGCMC	1 cut(s)	1261
BstUI	CGCG	3 cut(s)	180, 258, 426
BstV1I	GCAGC	7 cut(s)	294, 322, 691, 706, 1136, 1297, 1699
BstV2I	GAAGAC	3 cut(s)	12, 48, 602
BstX2I	RGATCY	2 cut(s)	1112, 1855
BstXI	CCANNNNNNTGG	1 cut(s)	1254
BstYI	RGATCY	2 cut(s)	1112, 1855
BstZI	CGGCCG	2 cut(s)	435, 1778
Bsu36I	CCTNAGG	1 cut(s)	1290
BsuI	GTATCC	2 cut(s)	1861, 2073
BtgI	CCRYGG	1 cut(s)	1508
BtgZI	GCGATG	1 cut(s)	2145
BtsCI	GGATG	8 cut(s)	33, 889, 904, 988, 1002, 1630, 1715, 2288
BtsIMutI	CAGTG	3 cut(s)	871, 893, 1164
BtuMI	TCGCGA	1 cut(s)	426
BveI	ACCTGC	1 cut(s)	1900
Cac8I	GCNNGC	6 cut(s)	182, 364, 533, 837, 1308, 2345
CciI	TCATGA	1 cut(s)	538
CfoI	GCGC	2 cut(s)	519, 757
Cfr10I	RCCGGY	1 cut(s)	649
Cfr13I	GGNCC	5 cut(s)	253, 403, 1257, 1258, 1559
CseI	GACGC	5 cut(s)	53, 313, 437, 463, 821
Csp6I	GTAC	3 cut(s)	85, 328, 385
CviQI	GTAC	3 cut(s)	85, 328, 385
DraIII	CACNNNGTG	2 cut(s)	346, 2191
DriI	GACNNNNNGTC	1 cut(s)	376
EaeI	YGGCCR	4 cut(s)	435, 932, 1481, 1778
EagI	CGGCCG	2 cut(s)	435, 1778
Eam1104I	CTCTTC	3 cut(s)	329, 408, 1656
Eam1105I	GACNNNNNGTC	1 cut(s)	376
EarI	CTCTTC	3 cut(s)	329, 408, 1656
EciI	GGCGGA	2 cut(s)	42, 209
Ecl136II	GAGCTC	4 cut(s)	175, 184, 508, 526
EclXI	CGGCCG	2 cut(s)	435, 1778
Eco130I	CCWWGG	1 cut(s)	1508
Eco147I	AGGCCT	1 cut(s)	2074
Eco24I	GRGCYC	6 cut(s)	177, 186, 510, 528, 946, 1261
Eco32I	GATATC	2 cut(s)	442, 2406
Eco47I	GGWCC	2 cut(s)	253, 403
Eco52I	CGGCCG	2 cut(s)	435, 1778
Eco53kI	GAGCTC	4 cut(s)	175, 184, 508, 526
Eco57I	CTGAAG	3 cut(s)	80, 656, 2415
Eco81I	CCTNAGG	1 cut(s)	1290
Eco88I	CYCGRG	1 cut(s)	245
EcoICRI	GAGCTC	4 cut(s)	175, 184, 508, 526
EcoNI	CCTNNNNNAGG	2 cut(s)	221, 1598
EcoRI	GAATTC	1 cut(s)	1413
EcoRII	CCWGG	4 cut(s)	222, 850, 1997, 2408
EcoRV	GATATC	2 cut(s)	442, 2406
EcoT14I	CCWWGG	1 cut(s)	1508
EcoT38I	GRGCYC	6 cut(s)	177, 186, 510, 528, 946, 1261
ErhI	CCWWGG	1 cut(s)	1508
Esp3I	CGTCTC	1 cut(s)	2302
FalI	AAGNNNNNCTT	2 cut(s)	584, 616
FaqI	GGGAC	2 cut(s)	283, 389
FauI	CCCGC	2 cut(s)	259, 1264
FbaI	TGATCA	1 cut(s)	1879
FblI	GTMKAC	1 cut(s)	860
FokI	GGATG	8 cut(s)	40, 896, 911, 975, 989, 1637, 1702, 2275
FriOI	GRGCYC	6 cut(s)	177, 186, 510, 528, 946, 1261
FspBI	CTAG	4 cut(s)	876, 1595, 1904, 2446
GlaI	GCGC	2 cut(s)	518, 756
GsaI	CCCAGC	1 cut(s)	1264
GsuI	CTGGAG	3 cut(s)	873, 1688, 2130
HapII	CCGG	7 cut(s)	71, 290, 389, 407, 434, 650, 947
HgaI	GACGC	5 cut(s)	53, 313, 437, 463, 821
HhaI	GCGC	2 cut(s)	519, 757
Hin1I	GRCGYC	2 cut(s)	45, 429
Hin6I	GCGC	2 cut(s)	517, 755
HinP1I	GCGC	2 cut(s)	517, 755
HincII	GTYRAC	1 cut(s)	2008
HindII	GTYRAC	1 cut(s)	2008
HindIII	AAGCTT	4 cut(s)	360, 545, 819, 1472
HinfI	GANTC	4 cut(s)	229, 553, 1460, 2135
HpaII	CCGG	7 cut(s)	71, 290, 389, 407, 434, 650, 947
HphI	GGTGA	7 cut(s)	133, 205, 1048, 1220, 1542, 1736, 2203
Hpy166II	GTNNAC	5 cut(s)	295, 739, 861, 1736, 2008
Hpy8I	GTNNAC	5 cut(s)	295, 739, 861, 1736, 2008
Hpy99I	CGWCG	3 cut(s)	307, 431, 457
HpyCH4III	ACNGT	3 cut(s)	969, 978, 1402
HpyCH4IV	ACGT	2 cut(s)	345, 1952
HpySE526I	ACGT	2 cut(s)	345, 1952
Hsp92I	GRCGYC	2 cut(s)	45, 429
HspAI	GCGC	2 cut(s)	517, 755
Ksp22I	TGATCA	1 cut(s)	1879
LguI	GCTCTTC	2 cut(s)	329, 1656
Lsp1109I	GCAGC	7 cut(s)	294, 322, 691, 706, 1136, 1297, 1699
MaeI	CTAG	4 cut(s)	876, 1595, 1904, 2446
MaeII	ACGT	2 cut(s)	345, 1952
MaeIII	GTNAC	5 cut(s)	127, 187, 211, 1368, 2199
MbiI	CCGCTC	1 cut(s)	50
MfeI	CAATTG	1 cut(s)	662
MflI	RGATCY	2 cut(s)	1112, 1855
MhlI	GDGCHC	7 cut(s)	177, 186, 510, 528, 946, 1261, 2318
MluCI	AATT	7 cut(s)	499, 662, 1104, 1275, 1413, 1535, 1915
MlyI	GAGTC	1 cut(s)	2129
MmeI	TCCRAC	4 cut(s)	552, 768, 1081, 1618
MroXI	GAANNNNTTC	3 cut(s)	93, 596, 1503
MseI	TTAA	6 cut(s)	357, 623, 1476, 1994, 2046, 2069
MslI	CAYNNNNRTG	3 cut(s)	194, 1014, 1252
MspA1I	CMGCKG	4 cut(s)	994, 1127, 1712, 2355
MspI	CCGG	7 cut(s)	71, 290, 389, 407, 434, 650, 947
MspR9I	CCNGG	8 cut(s)	72, 224, 389, 407, 434, 852, 1999, 2410
MunI	CAATTG	1 cut(s)	662
Mva1269I	GAATGC	2 cut(s)	1937, 2429
MvaI	CCWGG	4 cut(s)	224, 852, 1999, 2410
MvnI	CGCG	3 cut(s)	180, 258, 426
NciI	CCSGG	4 cut(s)	72, 389, 407, 434
NcoI	CCATGG	1 cut(s)	1508
NlaIV	GGNNCC	9 cut(s)	288, 405, 450, 849, 945, 1114, 1259, 1299, 1617
NmuCI	GTSAC	4 cut(s)	127, 187, 211, 1368
NruI	TCGCGA	1 cut(s)	426
NspI	RCATGY	1 cut(s)	1901
PaeR7I	CTCGAG	1 cut(s)	245
PagI	TCATGA	1 cut(s)	538
PceI	AGGCCT	1 cut(s)	2074
PciI	ACATGT	1 cut(s)	1897
PciSI	GCTCTTC	2 cut(s)	329, 1656
PcsI	WCGNNNNNNNCGW	2 cut(s)	278, 435
PctI	GAATGC	2 cut(s)	1937, 2429
PdmI	GAANNNNTTC	3 cut(s)	93, 596, 1503
PfeI	GAWTC	3 cut(s)	229, 553, 1460
Pfl23II	CGTACG	1 cut(s)	84
PflFI	GACNNNGTC	1 cut(s)	457
PflMI	CCANNNNNTGG	1 cut(s)	1267
PfoI	TCCNGGA	2 cut(s)	405, 2408
PleI	GAGTC	1 cut(s)	2129
PpsI	GAGTC	1 cut(s)	2129
Ppu21I	YACGTR	1 cut(s)	346
PscI	ACATGT	1 cut(s)	1897
PshAI	GACNNNNGTC	1 cut(s)	1780
PsiI	TTATAA	1 cut(s)	1833
Psp124BI	GAGCTC	4 cut(s)	177, 186, 510, 528
Psp6I	CCWGG	4 cut(s)	222, 850, 1997, 2408
PspFI	CCCAGC	1 cut(s)	1260
PspGI	CCWGG	4 cut(s)	222, 850, 1997, 2408
PspLI	CGTACG	1 cut(s)	84
PspN4I	GGNNCC	9 cut(s)	288, 405, 450, 849, 945, 1114, 1259, 1299, 1617
PspOMI	GGGCCC	1 cut(s)	1257
PspPI	GGNCC	5 cut(s)	253, 403, 1257, 1258, 1559
PspXI	VCTCGAGB	1 cut(s)	245
PstI	CTGCAG	2 cut(s)	312, 709
PsuI	RGATCY	2 cut(s)	1112, 1855
PsyI	GACNNNGTC	1 cut(s)	457
PvuII	CAGCTG	1 cut(s)	1712
RruI	TCGCGA	1 cut(s)	426
RsaI	GTAC	3 cut(s)	86, 329, 386
RsaNI	GTAC	3 cut(s)	85, 328, 385
RseI	CAYNNNNRTG	3 cut(s)	194, 1014, 1252
SacI	GAGCTC	4 cut(s)	177, 186, 510, 528
SapI	GCTCTTC	2 cut(s)	329, 1656
SaqAI	TTAA	6 cut(s)	357, 623, 1476, 1994, 2046, 2069
Sau96I	GGNCC	5 cut(s)	253, 403, 1257, 1258, 1559
SchI	GAGTC	1 cut(s)	2129
ScrFI	CCNGG	8 cut(s)	72, 224, 389, 407, 434, 852, 1999, 2410
SduI	GDGCHC	7 cut(s)	177, 186, 510, 528, 946, 1261, 2318
SfcI	CTRYAG	2 cut(s)	308, 705
Sfr274I	CTCGAG	1 cut(s)	245
SinI	GGWCC	2 cut(s)	253, 403
SlaI	CTCGAG	1 cut(s)	245
SmiMI	CAYNNNNRTG	3 cut(s)	194, 1014, 1252
SmlI	CTYRAG	7 cut(s)	245, 488, 548, 689, 816, 1061, 1172
SmoI	CTYRAG	7 cut(s)	245, 488, 548, 689, 816, 1061, 1172
SpeI	ACTAGT	1 cut(s)	875
Sse9I	AATT	7 cut(s)	499, 662, 1104, 1275, 1413, 1535, 1915
SseBI	AGGCCT	1 cut(s)	2074
SspI	AATATT	1 cut(s)	1982
SspMI	CTAG	4 cut(s)	876, 1595, 1904, 2446
SstI	GAGCTC	4 cut(s)	177, 186, 510, 528
StuI	AGGCCT	1 cut(s)	2074
StyD4I	CCNGG	8 cut(s)	70, 222, 387, 405, 432, 850, 1997, 2408
StyI	CCWWGG	1 cut(s)	1508
TaaI	ACNGT	3 cut(s)	969, 978, 1402
TaiI	ACGT	2 cut(s)	348, 1955
TaqI	TCGA	6 cut(s)	236, 246, 396, 461, 1458, 2268
TasI	AATT	7 cut(s)	499, 662, 1104, 1275, 1413, 1535, 1915
TauI	GCSGC	4 cut(s)	50, 58, 61, 934
TfiI	GAWTC	3 cut(s)	229, 553, 1460
Tru1I	TTAA	6 cut(s)	357, 623, 1476, 1994, 2046, 2069
Tru9I	TTAA	6 cut(s)	357, 623, 1476, 1994, 2046, 2069
TscAI	CASTG	3 cut(s)	871, 900, 1171
TseFI	GTSAC	4 cut(s)	127, 187, 211, 1368
TseI	GCWGC	7 cut(s)	307, 310, 694, 704, 1124, 1310, 1712
Tsp45I	GTSAC	4 cut(s)	127, 187, 211, 1368
TspDTI	ATGAA	9 cut(s)	545, 739, 789, 977, 998, 1242, 1484, 1860, 2166
TspGWI	ACGGA	2 cut(s)	287, 2326
TspRI	CASTG	3 cut(s)	871, 900, 1171
Tth111I	GACNNNGTC	1 cut(s)	457
Van91I	CCANNNNNTGG	1 cut(s)	1267
VpaK11BI	GGWCC	2 cut(s)	253, 403
XagI	CCTNNNNNAGG	2 cut(s)	221, 1598
XapI	RAATTY	2 cut(s)	1413, 1915
XceI	RCATGY	1 cut(s)	1901
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1201
XhoI	CTCGAG	1 cut(s)	245
XmiI	GTMKAC	1 cut(s)	860
XmnI	GAANNNNTTC	3 cut(s)	93, 596, 1503
XspI	CTAG	4 cut(s)	876, 1595, 1904, 2446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.