Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0017250

Physical Location & Seq

Genomic Coordinates Forward (+)

6153121 .. 6156696

Gene Structure 3576 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1088200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGATTCGAGTGCCTCAGTTCTCTTGTCCTCTCCATCAAAGCTCGAAAACCATCAGGAAACCAATAGCCATATCCAAAAGGGAGGTATTGCTCCTTTCATTGACTCATCATATTTGCAAAAACAATCCCATGTCCCCACTGGTTTCATTTGGCCTGAAAGTCACTTGGTTGCTGCTCTTGAGGAGCTCAATGAACCATTGGTGGATCTTGAGGGTTTCTTCAAGGGTGATGCTGTGGCAACCGAGCATGCTGCCAAACTCATTAGGGCATCTTGCTTGAGCCATGGTTTTTTCCAAGTGACCAACCACGGCGTTGATCCAAATCTCCTCAAACTCGCTGATGATCACATGGATCATTTCTTCAAGCTCCCCGTCGAAGAGAAAGCAAAGGTGAAAAGGGTACCTGGTAGCCCTTGGGGTTATTCTGGGTCTCATGCTGATCGATTCTCAACCAATTTGCCATGGAAGGAAACTTTGTCTTTTGGTTTCCAAGCGAATGGCACCGACACAGTTGTGGCGGATTTCTTCAAATCCAAACTAGGCGAAGATTTTGAACAAACAGGGCTGGTCTATCAAAAGTACTGTGAATCAATGAATGTTTTGGCTCTTTCAATAATGGAGCTGCTTGCAATCAGCTTGGGAGTGGATCGGATGCATTACAGAGAATTTTTTGAGGATGCTCACTCAATCATGAGGTGCAACTTCTACCCGACTTGCCAAGAGCCAAGTCTTACTCTCGGAACCGGACCTCATTGTGATCCAACATCCTTGACCATACTCAATCAAGACCAAGTTGGAGGGCTTGATGTGTTTGTCAACAAGAAGTGGCATTCGGTTCGACCGATTCAAGGTGCCCTAGTCATCAACATTGGTGACACCTTCTCGGCATTATCGAATGGGAAATACAAGAGTTGCTTGCACAGGGCAATGGTGAACAGGCGCAAGGAGAGGAGATCGTTGGCATTCTTTTTGTGCCCAAGGGATGACAAGGTTGTGAAGCCCCCCGAGGATCTTCTGCGCAAAGAACAAGAGACAAGAAAGTACCCGGATTTCACATGGTCAGATTTGCTCCGATTCACTCAATACCATTACAGGGCTGATGAAGCTACCCTCCAAAACTTCACCAATTGGTTCCTATCTGAATCCAACTCCACCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0045544 GO:0046394 GO:0048367 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0080167 GO:0090567 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01100 ko01110 map00904 map01100 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

387

Amino Acids

43.7

Weight (kDa)

6.06

Isoelectric Point (pI)

35.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	66 - 167	5.6e-22	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	228 - 326	6.8e-33	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017250)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35050
malus_domestica	MD15G1088200.v1.1
prunus_persica	Prupe.1G442200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0306941
rosa_laevigata	RLG00000010753
rosa_multiflora	Rmu_sc0007920.1_g000031
rosa_roxburghii	Rroxscaffold_7G00161340
rosa_rugosa	Rorug06G0354600.1
rosa_samantha	Rh6AG466300 Rh6BG434800 Rh6CG481000 Rh6DG467500
rosa_wichuraiana	Rw6G040620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1019
Acc65I	GGTACC	1 cut(s)	400
AccB1I	GGYRCC	3 cut(s)	400, 500, 851
AciI	CCGC	1 cut(s)	518
AclWI	GGATC	6 cut(s)	213, 311, 360, 654, 752, 1017
AcsI	RAATTY	1 cut(s)	665
AfaI	GTAC	3 cut(s)	402, 581, 1043
AfiI	CCNNNNNNNGG	2 cut(s)	848, 1093
AgsI	TTSAA	6 cut(s)	223, 364, 529, 554, 612, 848
AjnI	CCWGG	1 cut(s)	403
AleI	CACNNNNGTG	1 cut(s)	512
AluBI	AGCT	6 cut(s)	43, 187, 367, 622, 636, 1106
AluI	AGCT	6 cut(s)	43, 187, 367, 622, 636, 1106
Alw21I	GWGCWC	1 cut(s)	189
Alw26I	GTCTC	2 cut(s)	435, 1025
AlwI	GGATC	6 cut(s)	213, 311, 360, 654, 752, 1017
Ama87I	CYCGRG	1 cut(s)	1004
AoxI	GGCC	1 cut(s)	152
ApeKI	GCWGC	3 cut(s)	173, 251, 622
ApoI	RAATTY	1 cut(s)	665
Asp718I	GGTACC	1 cut(s)	400
AspLEI	GCGC	2 cut(s)	942, 1020
AspS9I	GGNCC	1 cut(s)	746
AsuC2I	CCSGG	1 cut(s)	1046
AsuHPI	GGTGA	5 cut(s)	239, 403, 884, 943, 1114
AvaI	CYCGRG	1 cut(s)	1004
AvaII	GGWCC	1 cut(s)	746
BaeGI	GKGCMC	2 cut(s)	856, 977
BanI	GGYRCC	3 cut(s)	400, 500, 851
BanII	GRGCYC	1 cut(s)	189
Bbv12I	GWGCWC	1 cut(s)	189
BbvI	GCAGC	3 cut(s)	160, 238, 609
BccI	CCATC	2 cut(s)	43, 60
BceAI	ACGGC	1 cut(s)	325
BcgI	CGANNNNNNTGC	4 cut(s)	233, 267, 818, 852
BciT130I	CCWGG	1 cut(s)	405
BclI	TGATCA	1 cut(s)	343
BcnI	CCSGG	1 cut(s)	1046
BcoDI	GTCTC	2 cut(s)	435, 1025
BfaI	CTAG	2 cut(s)	539, 857
BisI	GCNGC	3 cut(s)	174, 252, 623
BlsI	GCNGC	3 cut(s)	175, 253, 624
BmcAI	AGTACT	1 cut(s)	581
Bme1390I	CCNGG	2 cut(s)	405, 1046
Bme18I	GGWCC	1 cut(s)	746
BmeT110I	CYCGRG	1 cut(s)	1004
BmgT120I	GGNCC	1 cut(s)	746
BmiI	GGNNCC	5 cut(s)	402, 502, 742, 853, 1133
BmrFI	CCNGG	2 cut(s)	405, 1046
BmsI	GCATC	4 cut(s)	220, 278, 642, 667
BpuEI	CTTGAG	3 cut(s)	200, 230, 298
BpuMI	CCSGG	1 cut(s)	1046
Bsa29I	ATCGAT	1 cut(s)	442
BsaBI	GATNNNNATC	1 cut(s)	321
BsaI	GGTCTC	1 cut(s)	435
BsaJI	CCNNGG	6 cut(s)	283, 307, 413, 461, 977, 1005
BsaWI	WCCGGW	1 cut(s)	743
Bsc4I	CCNNNNNNNGG	2 cut(s)	848, 1093
Bse1I	ACTGG	1 cut(s)	145
Bse3DI	GCAATG	1 cut(s)	933
Bse8I	GATNNNNATC	1 cut(s)	321
BseBI	CCWGG	1 cut(s)	405
BseCI	ATCGAT	1 cut(s)	442
BseDI	CCNNGG	6 cut(s)	283, 307, 413, 461, 977, 1005
BseGI	GGATG	4 cut(s)	657, 682, 764, 988
BseJI	GATNNNNATC	1 cut(s)	321
BseLI	CCNNNNNNNGG	2 cut(s)	848, 1093
BseMI	GCAATG	1 cut(s)	933
BseMII	CTCAG	1 cut(s)	30
BseNI	ACTGG	1 cut(s)	145
BseRI	GAGGAG	3 cut(s)	197, 317, 964
BseSI	GKGCMC	2 cut(s)	856, 977
BseXI	GCAGC	3 cut(s)	160, 238, 609
Bsh1285I	CGRYCG	1 cut(s)	842
BshFI	GGCC	1 cut(s)	154
BshNI	GGYRCC	3 cut(s)	400, 500, 851
BshVI	ATCGAT	1 cut(s)	442
BsiEI	CGRYCG	1 cut(s)	842
BsiHKAI	GWGCWC	1 cut(s)	189
BsiHKCI	CYCGRG	1 cut(s)	1004
BsiSI	CCGG	2 cut(s)	744, 1046
BslFI	GGGAC	1 cut(s)	119
BslI	CCNNNNNNNGG	2 cut(s)	848, 1093
BsmAI	GTCTC	2 cut(s)	435, 1025
BsmFI	GGGAC	1 cut(s)	119
BsmI	GAATGC	2 cut(s)	829, 962
BsnI	GGCC	1 cut(s)	154
Bso31I	GGTCTC	1 cut(s)	435
BsoBI	CYCGRG	1 cut(s)	1004
Bsp1286I	GDGCHC	3 cut(s)	189, 856, 977
Bsp143I	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
Bsp19I	CCATGG	2 cut(s)	283, 461
BspACI	CCGC	1 cut(s)	518
BspANI	GGCC	1 cut(s)	154
BspCNI	CTCAG	1 cut(s)	29
BspDI	ATCGAT	1 cut(s)	442
BspHI	TCATGA	1 cut(s)	690
BspLI	GGNNCC	5 cut(s)	402, 502, 742, 853, 1133
BspPI	GGATC	6 cut(s)	213, 311, 360, 654, 752, 1017
BspT107I	GGYRCC	3 cut(s)	400, 500, 851
BspTNI	GGTCTC	1 cut(s)	435
BsrDI	GCAATG	1 cut(s)	933
BsrI	ACTGG	1 cut(s)	145
BssECI	CCNNGG	6 cut(s)	283, 307, 413, 461, 977, 1005
BssMI	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
BssT1I	CCWWGG	4 cut(s)	283, 413, 461, 977
Bst2UI	CCWGG	1 cut(s)	405
Bst4CI	ACNGT	2 cut(s)	511, 584
Bst6I	CTCTTC	1 cut(s)	372
BstC8I	GCNNGC	3 cut(s)	249, 627, 917
BstDEI	CTNAG	1 cut(s)	16
BstDSI	CCRYGG	3 cut(s)	283, 307, 461
BstF5I	GGATG	4 cut(s)	657, 682, 764, 988
BstHHI	GCGC	2 cut(s)	942, 1020
BstKTI	GATC	9 cut(s)	208, 319, 346, 355, 442, 649, 760, 956, 1012
BstMAI	GTCTC	2 cut(s)	435, 1025
BstMBI	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
BstMCI	CGRYCG	1 cut(s)	842
BstMWI	GCNNNNNNNGC	1 cut(s)	1103
BstNI	CCWGG	1 cut(s)	405
BstNSI	RCATGY	1 cut(s)	251
BstSCI	CCNGG	2 cut(s)	403, 1044
BstSLI	GKGCMC	2 cut(s)	856, 977
BstV1I	GCAGC	3 cut(s)	160, 238, 609
BstX2I	RGATCY	2 cut(s)	205, 1009
BstXI	CCANNNNNNTGG	1 cut(s)	497
BstYI	RGATCY	2 cut(s)	205, 1009
Bsu15I	ATCGAT	1 cut(s)	442
BsuRI	GGCC	1 cut(s)	154
BsuTUI	ATCGAT	1 cut(s)	442
BtgI	CCRYGG	3 cut(s)	283, 307, 461
BtsCI	GGATG	4 cut(s)	657, 682, 764, 988
BtsIMutI	CAGTG	1 cut(s)	138
Cac8I	GCNNGC	3 cut(s)	249, 627, 917
CciI	TCATGA	1 cut(s)	690
CfoI	GCGC	2 cut(s)	942, 1020
Cfr13I	GGNCC	1 cut(s)	746
ClaI	ATCGAT	1 cut(s)	442
CsiI	ACCWGGT	1 cut(s)	403
Csp6I	GTAC	3 cut(s)	401, 580, 1042
CviAII	CATG	8 cut(s)	131, 248, 284, 349, 434, 462, 691, 1056
CviQI	GTAC	3 cut(s)	401, 580, 1042
DdeI	CTNAG	1 cut(s)	16
DpnI	GATC	9 cut(s)	207, 318, 345, 354, 441, 648, 759, 955, 1011
DpnII	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
Eam1104I	CTCTTC	1 cut(s)	372
EarI	CTCTTC	1 cut(s)	372
EciI	GGCGGA	1 cut(s)	533
Ecl136II	GAGCTC	1 cut(s)	187
Eco130I	CCWWGG	4 cut(s)	283, 413, 461, 977
Eco24I	GRGCYC	1 cut(s)	189
Eco31I	GGTCTC	1 cut(s)	435
Eco47I	GGWCC	1 cut(s)	746
Eco53kI	GAGCTC	1 cut(s)	187
Eco88I	CYCGRG	1 cut(s)	1004
EcoICRI	GAGCTC	1 cut(s)	187
EcoRII	CCWGG	1 cut(s)	403
EcoT14I	CCWWGG	4 cut(s)	283, 413, 461, 977
EcoT22I	ATGCAT	1 cut(s)	657
EcoT38I	GRGCYC	1 cut(s)	189
ErhI	CCWWGG	4 cut(s)	283, 413, 461, 977
FaeI	CATG	8 cut(s)	134, 251, 287, 352, 437, 465, 694, 1059
FalI	AAGNNNNNCTT	2 cut(s)	899, 931
FaqI	GGGAC	1 cut(s)	119
FatI	CATG	8 cut(s)	130, 247, 283, 348, 433, 461, 690, 1055
FbaI	TGATCA	1 cut(s)	343
Fnu4HI	GCNGC	3 cut(s)	174, 252, 623
FokI	GGATG	4 cut(s)	664, 689, 751, 995
FriOI	GRGCYC	1 cut(s)	189
Fsp4HI	GCNGC	3 cut(s)	174, 252, 623
FspBI	CTAG	2 cut(s)	539, 857
FspI	TGCGCA	1 cut(s)	1019
GlaI	GCGC	2 cut(s)	941, 1019
GluI	GCNGC	3 cut(s)	174, 252, 623
HaeIII	GGCC	1 cut(s)	154
HapII	CCGG	2 cut(s)	744, 1046
HhaI	GCGC	2 cut(s)	942, 1020
Hin1II	CATG	8 cut(s)	134, 251, 287, 352, 437, 465, 694, 1059
Hin6I	GCGC	2 cut(s)	940, 1018
HinP1I	GCGC	2 cut(s)	940, 1018
HincII	GTYRAC	1 cut(s)	817
HindII	GTYRAC	1 cut(s)	817
HinfI	GANTC	7 cut(s)	5, 104, 444, 587, 844, 1074, 1142
HpaII	CCGG	2 cut(s)	744, 1046
HphI	GGTGA	5 cut(s)	239, 403, 884, 943, 1114
Hpy166II	GTNNAC	2 cut(s)	817, 934
Hpy188I	TCNGA	5 cut(s)	651, 740, 1063, 1073, 1141
Hpy188III	TCNNGA	5 cut(s)	56, 179, 209, 691, 785
Hpy8I	GTNNAC	2 cut(s)	817, 934
Hpy99I	CGWCG	1 cut(s)	377
HpyAV	CCTTC	2 cut(s)	460, 889
HpyCH4III	ACNGT	2 cut(s)	511, 584
HpyCH4V	TGCA	5 cut(s)	118, 629, 655, 699, 919
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1103
HpyF3I	CTNAG	1 cut(s)	16
Hsp92II	CATG	8 cut(s)	134, 251, 287, 352, 437, 465, 694, 1059
HspAI	GCGC	2 cut(s)	940, 1018
KpnI	GGTACC	1 cut(s)	404
Ksp22I	TGATCA	1 cut(s)	343
Kzo9I	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
LmnI	GCTCC	5 cut(s)	97, 184, 372, 619, 1074
Lsp1109I	GCAGC	3 cut(s)	160, 238, 609
LweI	GCATC	4 cut(s)	220, 278, 642, 667
MabI	ACCWGGT	1 cut(s)	403
MaeI	CTAG	2 cut(s)	539, 857
MaeIII	GTNAC	3 cut(s)	161, 298, 872
MalI	GATC	9 cut(s)	207, 318, 345, 354, 441, 648, 759, 955, 1011
MboI	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
MboII	GAAGA	6 cut(s)	211, 352, 389, 517, 557, 1004
MfeI	CAATTG	1 cut(s)	1126
MflI	RGATCY	2 cut(s)	205, 1009
MhlI	GDGCHC	3 cut(s)	189, 856, 977
MluCI	AATT	4 cut(s)	454, 665, 1126, 1156
MlyI	GAGTC	1 cut(s)	98
MmeI	TCCRAC	2 cut(s)	775, 785
Mph1103I	ATGCAT	1 cut(s)	657
MseI	TTAA	1 cut(s)	1159
MslI	CAYNNNNRTG	1 cut(s)	512
MspI	CCGG	2 cut(s)	744, 1046
MspR9I	CCNGG	2 cut(s)	405, 1046
MunI	CAATTG	1 cut(s)	1126
Mva1269I	GAATGC	2 cut(s)	829, 962
MvaI	CCWGG	1 cut(s)	405
MwoI	GCNNNNNNNGC	1 cut(s)	1103
NciI	CCSGG	1 cut(s)	1046
NcoI	CCATGG	2 cut(s)	283, 461
NdeII	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
NlaIII	CATG	8 cut(s)	134, 251, 287, 352, 437, 465, 694, 1059
NlaIV	GGNNCC	5 cut(s)	402, 502, 742, 853, 1133
NmeAIII	GCCGAG	1 cut(s)	863
NmuCI	GTSAC	3 cut(s)	161, 298, 872
NsbI	TGCGCA	1 cut(s)	1019
NsiI	ATGCAT	1 cut(s)	657
NspI	RCATGY	1 cut(s)	251
OliI	CACNNNNGTG	1 cut(s)	512
PaeI	GCATGC	1 cut(s)	251
PagI	TCATGA	1 cut(s)	690
PcsI	WCGNNNNNNNCGW	2 cut(s)	839, 890
PctI	GAATGC	2 cut(s)	829, 962
PfeI	GAWTC	6 cut(s)	5, 444, 587, 844, 1074, 1142
PkrI	GCNGC	3 cut(s)	175, 253, 624
PleI	GAGTC	1 cut(s)	98
PpsI	GAGTC	1 cut(s)	98
Psp124BI	GAGCTC	1 cut(s)	189
Psp6I	CCWGG	1 cut(s)	403
PspGI	CCWGG	1 cut(s)	403
PspN4I	GGNNCC	5 cut(s)	402, 502, 742, 853, 1133
PspPI	GGNCC	1 cut(s)	746
PsuI	RGATCY	2 cut(s)	205, 1009
RsaI	GTAC	3 cut(s)	402, 581, 1043
RsaNI	GTAC	3 cut(s)	401, 580, 1042
RseI	CAYNNNNRTG	1 cut(s)	512
SacI	GAGCTC	1 cut(s)	189
SaqAI	TTAA	1 cut(s)	1159
SatI	GCNGC	3 cut(s)	174, 252, 623
Sau3AI	GATC	9 cut(s)	205, 316, 343, 352, 439, 646, 757, 953, 1009
Sau96I	GGNCC	1 cut(s)	746
ScaI	AGTACT	1 cut(s)	581
SchI	GAGTC	1 cut(s)	98
ScrFI	CCNGG	2 cut(s)	405, 1046
SduI	GDGCHC	3 cut(s)	189, 856, 977
SexAI	ACCWGGT	1 cut(s)	403
SfaNI	GCATC	4 cut(s)	220, 278, 642, 667
SinI	GGWCC	1 cut(s)	746
SmiMI	CAYNNNNRTG	1 cut(s)	512
SmlI	CTYRAG	3 cut(s)	179, 209, 277
SmoI	CTYRAG	3 cut(s)	179, 209, 277
SphI	GCATGC	1 cut(s)	251
Sse9I	AATT	4 cut(s)	454, 665, 1126, 1156
SsiI	CCGC	1 cut(s)	518
SspMI	CTAG	2 cut(s)	539, 857
SstI	GAGCTC	1 cut(s)	189
StyD4I	CCNGG	2 cut(s)	403, 1044
StyI	CCWWGG	4 cut(s)	283, 413, 461, 977
TaaI	ACNGT	2 cut(s)	511, 584
TaqI	TCGA	6 cut(s)	8, 45, 375, 442, 838, 893
TaqII	GACCGA	1 cut(s)	856
TasI	AATT	4 cut(s)	454, 665, 1126, 1156
TatI	WGTACW	1 cut(s)	579
TfiI	GAWTC	6 cut(s)	5, 444, 587, 844, 1074, 1142
Tru1I	TTAA	1 cut(s)	1159
Tru9I	TTAA	1 cut(s)	1159
TscAI	CASTG	1 cut(s)	145
TseFI	GTSAC	3 cut(s)	161, 298, 872
TseI	GCWGC	3 cut(s)	173, 251, 622
Tsp45I	GTSAC	3 cut(s)	161, 298, 872
TspDTI	ATGAA	5 cut(s)	88, 136, 207, 608, 1116
TspRI	CASTG	1 cut(s)	145
VpaK11BI	GGWCC	1 cut(s)	746
XapI	RAATTY	1 cut(s)	665
XceI	RCATGY	1 cut(s)	251
XcmI	CCANNNNNNNNNTGG	1 cut(s)	137
XspI	CTAG	2 cut(s)	539, 857
ZrmI	AGTACT	1 cut(s)	581
Zsp2I	ATGCAT	1 cut(s)	657

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.