MD15G1090400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0019758
Physical Location & Seq
Forward (+)
6277999 .. 6279300
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1090400.v1.1.491

Sequence Viewer

Length: 549 bp
ATGCCGGATGGTTGGATTGGAGGTTTGAGTTTACTTCGAACTTTGTCCATTGAAAGCTGCACTAATCTCACTTCTCTGTCATCAAGCTTGGAAAAGCTCGCGTTGCTTGAGCACTTGACAATTATGTTCTGTCCAAAACTTGGTTCTTTTCCAGAAGGTGTTCAACACCTCTCCTCCCTTCGAAGTTTGATCCTGCTGGGCAATCCTTGGTTTGATTCTCTTCCAGAAGGGTTACAAAATGTGGGAACACTGCACTGTCTCGAAATTGGTAGCTGCCCCAATCTAACTGCTTTGCCAGAATGGTTTGAGGGTCTTGATTCTCTTAGGTCCTTGACGATATCTGATTGCCCCGGTTTACAAGTATTGCCACCAGGTTTCAAGATTCTCACTAAGCTCCAACACCTCTCTATTCAAGAATGCCCGGAGCTTGAGGAAAGATGTAAACAAGGTAGTGGTGAGGATTGGATGAAAATAGCCCACGTCCCACATAAGTTCATCGGACCGCCTCAAGCCAGGCAGTCCGGTGAAGCGAGCACATCTGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

183

Amino Acids

19.79

Weight (kDa)

5.29

Isoelectric Point (pI)

67.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 54 - 140 6.6e-06 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 76 - 140 5.1e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019758)

Species Orthologous Gene IDs
malus_domestica MD15G1090400.v1.1
rosa_laevigata RLG00000013872
rosa_multiflora Rmu_co7995590.1_g000001 Rmu_sc0039888.1_g000001 Rmu_ssc0000119.1_g000021
rosa_rugosa Rorug02G0470500
rosa_samantha Rh1DG209000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 140
AccII CGCG 1 cut(s) 101
AciI CCGC 1 cut(s) 503
AclWI GGATC 1 cut(s) 184
AfiI CCNNNNNNNGG 1 cut(s) 140
AgsI TTSAA 4 cut(s) 53, 164, 379, 413
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 2 cut(s) 370, 512
AjuI GAANNNNNNNTTGG 2 cut(s) 127, 159
AluBI AGCT 7 cut(s) 57, 87, 97, 273, 394, 427, 546
AluI AGCT 7 cut(s) 57, 87, 97, 273, 394, 427, 546
Alw21I GWGCWC 2 cut(s) 114, 536
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 184
ApeKI GCWGC 3 cut(s) 57, 273, 543
Asp700I GAANNNNTTC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 327, 500
AsuC2I CCSGG 2 cut(s) 351, 422
AsuHPI GGTGA 2 cut(s) 467, 536
AsuII TTCGAA 2 cut(s) 37, 181
AvaII GGWCC 2 cut(s) 327, 500
Bbv12I GWGCWC 2 cut(s) 114, 536
BbvI GCAGC 2 cut(s) 44, 260
BccI CCATC 1 cut(s) 2
BciT130I CCWGG 2 cut(s) 372, 514
BcnI CCSGG 2 cut(s) 351, 422
BcoDI GTCTC 1 cut(s) 263
BisI GCNGC 3 cut(s) 58, 274, 544
BlsI GCNGC 3 cut(s) 59, 275, 545
Bme1390I CCNGG 4 cut(s) 351, 372, 422, 514
Bme18I GGWCC 2 cut(s) 327, 500
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 2 cut(s) 327, 500
BmrFI CCNGG 4 cut(s) 351, 372, 422, 514
Bpu14I TTCGAA 2 cut(s) 37, 181
BpuEI CTTGAG 3 cut(s) 128, 449, 492
BpuMI CCSGG 2 cut(s) 351, 422
BsaJI CCNNGG 2 cut(s) 206, 349
BsaWI WCCGGW 1 cut(s) 521
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 140
BseBI CCWGG 2 cut(s) 372, 514
BseDI CCNNGG 2 cut(s) 206, 349
BseGI GGATG 2 cut(s) 13, 471
BseLI CCNNNNNNNGG 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 163
BseXI GCAGC 2 cut(s) 44, 260
BseYI CCCAGC 1 cut(s) 196
BsgI GTGCAG 2 cut(s) 43, 236
Bsh1236I CGCG 1 cut(s) 101
BsiHKAI GWGCWC 2 cut(s) 114, 536
BsiSI CCGG 4 cut(s) 5, 351, 422, 522
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 422
Bsp119I TTCGAA 2 cut(s) 37, 181
Bsp1286I GDGCHC 2 cut(s) 114, 536
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 1 cut(s) 503
BspFNI CGCG 1 cut(s) 101
BspPI GGATC 1 cut(s) 184
BspT104I TTCGAA 2 cut(s) 37, 181
BssECI CCNNGG 2 cut(s) 206, 349
BssMI GATC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 372, 514
Bst4CI ACNGT 1 cut(s) 257
Bst6I CTCTTC 1 cut(s) 225
BstBI TTCGAA 2 cut(s) 37, 181
BstC8I GCNNGC 2 cut(s) 99, 532
BstDEI CTNAG 2 cut(s) 323, 390
BstF5I GGATG 2 cut(s) 13, 471
BstFNI CGCG 1 cut(s) 101
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 2 cut(s) 103, 540
BstNI CCWGG 2 cut(s) 372, 514
BstSCI CCNGG 4 cut(s) 349, 370, 420, 512
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 2 cut(s) 44, 260
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 2 cut(s) 13, 471
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 2 cut(s) 248, 253
Cac8I GCNNGC 2 cut(s) 99, 532
Cfr13I GGNCC 2 cut(s) 327, 500
CpoI CGGWCCG 1 cut(s) 500
CsiI ACCWGGT 1 cut(s) 370
CspI CGGWCCG 1 cut(s) 500
CviJI RGCY 9 cut(s) 57, 87, 97, 273, 394, 427, 476, 512, 546
CviKI_1 RGCY 9 cut(s) 57, 87, 97, 273, 394, 427, 476, 512, 546
DdeI CTNAG 2 cut(s) 323, 390
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 206
Eco32I GATATC 1 cut(s) 339
Eco47I GGWCC 2 cut(s) 327, 500
EcoO109I RGGNCCY 1 cut(s) 327
EcoRII CCWGG 2 cut(s) 370, 512
EcoRV GATATC 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 206
FaiI YATR 2 cut(s) 125, 489
FaqI GGGAC 1 cut(s) 467
Fnu4HI GCNGC 3 cut(s) 58, 274, 544
FokI GGATG 2 cut(s) 20, 478
Fsp4HI GCNGC 3 cut(s) 58, 274, 544
GluI GCNGC 3 cut(s) 58, 274, 544
GsaI CCCAGC 1 cut(s) 200
HapII CCGG 4 cut(s) 5, 351, 422, 522
HindIII AAGCTT 1 cut(s) 85
HinfI GANTC 3 cut(s) 215, 317, 382
HpaII CCGG 4 cut(s) 5, 351, 422, 522
HphI GGTGA 2 cut(s) 467, 536
Hpy166II GTNNAC 3 cut(s) 32, 356, 443
Hpy188I TCNGA 2 cut(s) 343, 500
Hpy188III TCNNGA 6 cut(s) 152, 224, 260, 314, 379, 413
Hpy8I GTNNAC 3 cut(s) 32, 356, 443
HpyAV CCTTC 3 cut(s) 149, 188, 221
HpyCH4III ACNGT 1 cut(s) 257
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 2 cut(s) 60, 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 540
HpyF3I CTNAG 2 cut(s) 323, 390
HpySE526I ACGT 1 cut(s) 480
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 2 cut(s) 399, 424
Lsp1109I GCAGC 2 cut(s) 44, 260
MabI ACCWGGT 1 cut(s) 370
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 1 cut(s) 231
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 1 cut(s) 212
MhlI GDGCHC 2 cut(s) 114, 536
MluCI AATT 2 cut(s) 120, 264
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 8 cut(s) 14, 179, 184, 301, 413, 424, 451, 516
MroXI GAANNNNTTC 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 546
MspI CCGG 4 cut(s) 5, 351, 422, 522
MspR9I CCNGG 4 cut(s) 351, 372, 422, 514
Mva1269I GAATGC 1 cut(s) 422
MvaI CCWGG 2 cut(s) 372, 514
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 103, 540
NciI CCSGG 2 cut(s) 351, 422
NdeII GATC 1 cut(s) 189
NspV TTCGAA 2 cut(s) 37, 181
PctI GAATGC 1 cut(s) 422
PdmI GAANNNNTTC 1 cut(s) 159
PfeI GAWTC 3 cut(s) 215, 317, 382
PflMI CCANNNNNTGG 1 cut(s) 140
PkrI GCNGC 3 cut(s) 59, 275, 545
PpuMI RGGWCCY 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 370, 512
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 2 cut(s) 370, 512
PspPI GGNCC 2 cut(s) 327, 500
PspPPI RGGWCCY 1 cut(s) 327
PvuII CAGCTG 1 cut(s) 546
Rsr2I CGGWCCG 1 cut(s) 500
RsrII CGGWCCG 1 cut(s) 500
SatI GCNGC 3 cut(s) 58, 274, 544
Sau3AI GATC 1 cut(s) 189
Sau96I GGNCC 2 cut(s) 327, 500
ScrFI CCNGG 4 cut(s) 351, 372, 422, 514
SduI GDGCHC 2 cut(s) 114, 536
SexAI ACCWGGT 1 cut(s) 370
SfuI TTCGAA 2 cut(s) 37, 181
SinI GGWCC 2 cut(s) 327, 500
SmlI CTYRAG 3 cut(s) 107, 428, 507
SmoI CTYRAG 3 cut(s) 107, 428, 507
Sse9I AATT 2 cut(s) 120, 264
SsiI CCGC 1 cut(s) 503
StyD4I CCNGG 4 cut(s) 349, 370, 420, 512
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 1 cut(s) 257
TaiI ACGT 1 cut(s) 483
TaqI TCGA 3 cut(s) 37, 181, 261
TasI AATT 2 cut(s) 120, 264
TfiI GAWTC 3 cut(s) 215, 317, 382
TscAI CASTG 2 cut(s) 255, 260
TseI GCWGC 3 cut(s) 57, 273, 543
TspDTI ATGAA 2 cut(s) 482, 484
TspRI CASTG 2 cut(s) 255, 260
Van91I CCANNNNNTGG 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 327, 500
XmnI GAANNNNTTC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.