Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0019758

Physical Location & Seq

Genomic Coordinates Forward (+)

60137734 .. 60149435

Gene Structure 11702 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0470500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGTGGAAGCTTAAGGTTGCAGAAGGTGGGGAAGAGTCCTCGGCTTACATTTACAGCACGAACAACTTTGTGGGAAGGCAGACATGGGAGTTTGATCCCGATGCAGGAACTGCTGAAGAGCGAGCTGAGGTTGAAGCGGCTCGTCTTCATTTCTACAACAACCGCCATCTGGTTAAGCCCAGTGGTGACCTTCTTTGGAGATTGCAGTTCCTAAGGGAGAAGCAGTTCAAACAGACAATTCCTCCAGTAAAAATTGAAGATGGCGAGGTTATCACATATGAAAAAACCACAGATGCACTGAGAAGGTCTGTCCACTTCTTCTCAGCGCTGCAAGCAAGCGATGGCCATTGGCCTGCGGAAAATGCTGGCCCTTTATTTTTCCTTCCTCCTATGGTCATAGTTATGCACATTACGGGGCATCTTAACAGTGTATTATCTGAGGAGCATCGCAAAGAAATTCTGCGTTACATATACTATCATCAGAATGAAGATGGTGGTTGGGGACTACATGTCGAAGGTCATAGCACCATGTTCAGCACAGCTCTCAGCTACATTTGTATGCGTATTCTCGGAGAAGGACCTGATGATGGTGGCCAAGACAATGCTTGTCCGAGAGCAAGAAAGTGGATTCTTGATCATGGTGGTGTCACACACATACCCTCTTGGGGAAAGACTTGGCTTTCGATTCTTGGTTTGTTTAATTGGTCTGGAAGCAACCCTATTCCCCCAGAGTTTTGGATTATTCCTTCATTTCTTCCTATGCATCCAGCAAAAATGTGGTGCTATAGTAGGACGTCTTACATGCCAATGTCATACCTATATGGAAAAAGGTTTGTTGGCCCAATTACACCCCTCATTCTTCAATTGAGGGACGAACTTTTTATTCAACCTTACAATGAAATCAATTGGAAGGAAGTACGCCATCTATGTGCCAAAGAGGATATCTACTACCCTCATCCTTGGATACAAGATCTCCTTTGGGATACCCTCTACATTTCCACAGAGCCTCTTCTTACCCGTTGGCCCTTCAACAAGTTGATCAGAGAAAAGGCTCTACAAGTGACGATGGAGCATATTCATTATGAAGATGAGAACAGCCGATACATTACCATGGGGTGTTTGGAAAAGGCGTTATGTATGCTTGCCTGTTGGGCTGAAGATCCAAATGGGGACTATTTCAAGAAGCATCTCGCTAGGATCCCGGATTATTTATGGGTTGCTGAAGATGGGATGAAGATGCAAAGTTTTGGCAGTCAGGAGTGGGATACTAGTTTTGCCATTCAAGCTTTGCTTGCTAGTAATCTCAAGGATGAAATTGGACCAACGCTTGCTAGAGGACATGACTTCATAAAGAAATCTCAGGTCAAGGACAACCCTTCTGGTGACTTCAAAAGCATGTACCGCCACATTTCTAAAGGATCGTGGACTTTCTCCGATCAAGATCATGGATGGCAAGTTTCTGATTGCACTGCAGAAGGTTTAAAGTGTTGTCTTCTGTTGTCGATGATGCCATCGGAGATAGTTGGTGAAAAAATGGAGCCTGAGCGCTTGTACGATTCTGTCAATGTTCTTCTTTCCCTACAGAGCAAGAATGGTGGTTTATCAGCCTGGGAGCCAGCAGGGGCAGCAGAATGGTTAGAACTGTTAAATCCGACAGAGTTCTTGGAGGACATTGTAATTGAGCATGAACATGTTGAGTGCACTTCATCTGTAATCCAGGCTTTAGTTCTGTTTAAGAAGCTACACCCTGGGCATAGGAAGAAAGAGATTGAACATTCCATCACCAATGCTGTACGCTACGTTGAAAATATGCAAATGCCGGATGGTTCATGGTATGGAGATTGGGGAGTTTGCTTCACATATGGTACCTGGTTTGCACTAGGAGGATTGGCAGCTGCTGGCAAGACTTTTAGCACTTGTGCAGCCATGCGCAATGGGGTTAGCTTTCTACTTACGGTGCAGAGAGAGAACGGTGGTTGGGGAGAGAGCTATCTTTCATGTCCACAAAAGACATACATTCCTCTCGAAGGAAACCGATCCAATCTAGTACATACTGCTTGGGCTATGATGGGTCTCATTCATGCGGGACAGGCAGAAAGAGACCCAACACCTCTTCATCGCGCAGCAAAGTTTATAATCAATTCTCAACTGGAAAATGGTGATTTTCCCCAGCAGGAAATCACTGGAGTCTTCAAGAAGAACTGCATGCTACATTTTGCAGCATATAGAAATATATTCCCACTGTGGGCTCTTGCAGAATATCGCAATTGTTTTCCCTTGCCTTCCAAGGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0043207 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

764

Amino Acids

87.4

Weight (kDa)

5.89

Isoelectric Point (pI)

44.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	101 - 404	1.3e-37	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	420 - 756	7.7e-51	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019758)

Species	Orthologous Gene IDs
malus_domestica	MD15G1090400.v1.1
rosa_laevigata	RLG00000013872
rosa_multiflora	Rmu_co7995590.1_g000001 Rmu_sc0039888.1_g000001 Rmu_ssc0000119.1_g000021
rosa_rugosa	Rorug02G0470500
rosa_samantha	Rh1DG209000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2135
AatII	GACGTC	1 cut(s)	797
Acc16I	TGCGCA	1 cut(s)	1931
Acc65I	GGTACC	1 cut(s)	1865
AccB1I	GGYRCC	1 cut(s)	1865
AccII	CGCG	1 cut(s)	2121
AciI	CCGC	5 cut(s)	137, 163, 356, 1402, 2084
AclWI	GGATC	6 cut(s)	89, 1154, 1192, 1205, 1426, 2031
AcoI	YGGCCR	2 cut(s)	343, 592
AcsI	RAATTY	1 cut(s)	456
AcuI	CTGAAG	3 cut(s)	135, 1176, 1242
AcyI	GRCGYC	1 cut(s)	794
AfaI	GTAC	6 cut(s)	918, 1400, 1553, 1794, 1867, 2049
AfeI	AGCGCT	2 cut(s)	327, 1547
AfiI	CCNNNNNNNGG	7 cut(s)	104, 169, 587, 665, 2027, 2245, 2288
AflII	CTTAAG	1 cut(s)	11
AflIII	ACRYGT	2 cut(s)	508, 1690
AhdI	GACNNNNNGTC	1 cut(s)	509
AhlI	ACTAGT	1 cut(s)	1268
AjnI	CCWGG	4 cut(s)	1607, 1716, 1747, 1868
AloI	GAACNNNNNNTCC	2 cut(s)	209, 241
AluBI	AGCT	9 cut(s)	10, 125, 542, 549, 1286, 1741, 1895, 1944, 1989
AluI	AGCT	9 cut(s)	10, 125, 542, 549, 1286, 1741, 1895, 1944, 1989
Alw21I	GWGCWC	1 cut(s)	1703
Alw26I	GTCTC	2 cut(s)	2078, 2094
Alw44I	GTGCAC	1 cut(s)	1699
AlwI	GGATC	6 cut(s)	89, 1154, 1192, 1205, 1426, 2031
AlwNI	CAGNNNCTG	2 cut(s)	110, 1898
Aor51HI	AGCGCT	2 cut(s)	327, 1547
AoxI	GGCC	6 cut(s)	343, 350, 367, 592, 838, 1022
ApaLI	GTGCAC	1 cut(s)	1699
ApeKI	GCWGC	7 cut(s)	328, 1625, 1892, 1895, 1922, 2123, 2219
ApoI	RAATTY	1 cut(s)	456
ArsI	GACNNNNNNTTYG	2 cut(s)	664, 696
Asp700I	GAANNNNTTC	1 cut(s)	224
Asp718I	GGTACC	1 cut(s)	1865
AspLEI	GCGC	4 cut(s)	328, 1548, 1932, 2123
AspS9I	GGNCC	5 cut(s)	368, 578, 839, 1023, 1319
AsuC2I	CCSGG	1 cut(s)	1202
AsuHPI	GGTGA	5 cut(s)	197, 1394, 1538, 1774, 2171
AvaII	GGWCC	2 cut(s)	578, 1319
AxyI	CCTNAGG	1 cut(s)	212
BaeGI	GKGCMC	1 cut(s)	1703
BalI	TGGCCA	2 cut(s)	345, 594
BamHI	GGATCC	1 cut(s)	1197
BanI	GGYRCC	1 cut(s)	1865
BanII	GRGCYC	1 cut(s)	2251
BbsI	GAAGAC	3 cut(s)	137, 1484, 2182
Bbv12I	GWGCWC	1 cut(s)	1703
BbvCI	CCTCAGC	1 cut(s)	126
BbvI	GCAGC	7 cut(s)	315, 1637, 1882, 1904, 1934, 2135, 2231
BciT130I	CCWGG	4 cut(s)	1609, 1718, 1749, 1870
BciVI	GTATCC	3 cut(s)	957, 976, 1258
BclI	TGATCA	2 cut(s)	634, 1038
BcnI	CCSGG	1 cut(s)	1202
BcoDI	GTCTC	2 cut(s)	2078, 2094
BcuI	ACTAGT	1 cut(s)	1268
BfaI	CTAG	6 cut(s)	1194, 1269, 1296, 1332, 1880, 2045
BfmI	CTRYAG	3 cut(s)	784, 1470, 1580
BfoI	RGCGCY	2 cut(s)	329, 1549
BfrI	CTTAAG	1 cut(s)	11
BfuI	GTATCC	3 cut(s)	957, 976, 1258
BglI	GCCNNNNNGGC	1 cut(s)	1151
BglII	AGATCT	1 cut(s)	970
BisI	GCNGC	8 cut(s)	138, 329, 1626, 1893, 1896, 1923, 2124, 2220
BlsI	GCNGC	8 cut(s)	139, 330, 1627, 1894, 1897, 1924, 2125, 2221
Bme1390I	CCNGG	5 cut(s)	1202, 1609, 1718, 1749, 1870
Bme18I	GGWCC	2 cut(s)	578, 1319
BmeRI	GACNNNNNGTC	1 cut(s)	509
BmgT120I	GGNCC	5 cut(s)	368, 578, 839, 1023, 1319
BmiI	GGNNCC	4 cut(s)	1199, 1539, 1614, 1867
BmrFI	CCNGG	5 cut(s)	1202, 1609, 1718, 1749, 1870
BmrI	ACTGGG	1 cut(s)	174
BmsI	GCATC	8 cut(s)	91, 283, 427, 454, 772, 1195, 1227, 1497
BmuI	ACTGGG	1 cut(s)	174
BpiI	GAAGAC	3 cut(s)	137, 1484, 2182
BpmI	CTGGAG	2 cut(s)	228, 2205
Bpu10I	CCTNAGC	2 cut(s)	126, 1542
BpuEI	CTTGAG	1 cut(s)	1289
BpuMI	CCSGG	1 cut(s)	1202
BsaBI	GATNNNNATC	1 cut(s)	1440
BsaHI	GRCGYC	1 cut(s)	794
BsaI	GGTCTC	2 cut(s)	2078, 2094
BsaJI	CCNNGG	7 cut(s)	39, 959, 1110, 1608, 1747, 1748, 2286
BsaXI	ACNNNNNCTCC	6 cut(s)	209, 226, 239, 256, 957, 987
Bsc4I	CCNNNNNNNGG	7 cut(s)	104, 169, 587, 665, 2027, 2245, 2288
Bse1I	ACTGG	4 cut(s)	180, 245, 2154, 2188
Bse21I	CCTNAGG	1 cut(s)	212
Bse3DI	GCAATG	1 cut(s)	1939
Bse8I	GATNNNNATC	1 cut(s)	1440
BseBI	CCWGG	4 cut(s)	1609, 1718, 1749, 1870
BseDI	CCNNGG	7 cut(s)	39, 959, 1110, 1608, 1747, 1748, 2286
BseGI	GGATG	6 cut(s)	763, 955, 1236, 1315, 1454, 1828
BseJI	GATNNNNATC	1 cut(s)	1440
BseLI	CCNNNNNNNGG	7 cut(s)	104, 169, 587, 665, 2027, 2245, 2288
BseMI	GCAATG	1 cut(s)	1939
BseMII	CTCAG	7 cut(s)	117, 290, 336, 429, 559, 1373, 1533
BseNI	ACTGG	4 cut(s)	180, 245, 2154, 2188
BseRI	GAGGAG	1 cut(s)	455
BseSI	GKGCMC	1 cut(s)	1703
BseXI	GCAGC	7 cut(s)	315, 1637, 1882, 1904, 1934, 2135, 2231
BseYI	CCCAGC	1 cut(s)	2169
BsgI	GTGCAG	2 cut(s)	1941, 1979
Bsh1236I	CGCG	1 cut(s)	2121
BshFI	GGCC	6 cut(s)	345, 352, 369, 594, 840, 1024
BshNI	GGYRCC	1 cut(s)	1865
BsiHKAI	GWGCWC	1 cut(s)	1703
BsiSI	CCGG	2 cut(s)	1202, 1820
BslFI	GGGAC	4 cut(s)	516, 884, 1184, 2100
BslI	CCNNNNNNNGG	7 cut(s)	104, 169, 587, 665, 2027, 2245, 2288
BsmAI	GTCTC	2 cut(s)	2078, 2094
BsmFI	GGGAC	4 cut(s)	516, 884, 1184, 2100
BsnI	GGCC	6 cut(s)	345, 352, 369, 594, 840, 1024
Bso31I	GGTCTC	2 cut(s)	2078, 2094
Bsp1286I	GDGCHC	2 cut(s)	1703, 2251
Bsp19I	CCATGG	1 cut(s)	1110
BspACI	CCGC	5 cut(s)	137, 163, 356, 1402, 2084
BspANI	GGCC	6 cut(s)	345, 352, 369, 594, 840, 1024
BspCNI	CTCAG	7 cut(s)	118, 291, 335, 430, 558, 1372, 1534
BspFNI	CGCG	1 cut(s)	2121
BspLI	GGNNCC	4 cut(s)	1199, 1539, 1614, 1867
BspMAI	CTGCAG	1 cut(s)	1474
BspPI	GGATC	6 cut(s)	89, 1154, 1192, 1205, 1426, 2031
BspQI	GCTCTTC	1 cut(s)	111
BspT107I	GGYRCC	1 cut(s)	1865
BspTI	CTTAAG	1 cut(s)	11
BspTNI	GGTCTC	2 cut(s)	2078, 2094
BsrDI	GCAATG	1 cut(s)	1939
BsrI	ACTGG	4 cut(s)	180, 245, 2154, 2188
BssECI	CCNNGG	7 cut(s)	39, 959, 1110, 1608, 1747, 1748, 2286
BssNI	GRCGYC	1 cut(s)	794
BssT1I	CCWWGG	3 cut(s)	959, 1110, 2286
Bst2UI	CCWGG	4 cut(s)	1609, 1718, 1749, 1870
Bst4CI	ACNGT	5 cut(s)	428, 1644, 1957, 1973, 2244
Bst6I	CTCTTC	4 cut(s)	27, 111, 1014, 2118
BstACI	GRCGYC	1 cut(s)	794
BstAFI	CTTAAG	1 cut(s)	11
BstAPI	GCANNNNNTGC	1 cut(s)	110
BstDEI	CTNAG	8 cut(s)	126, 212, 299, 322, 438, 545, 1359, 1542
BstDSI	CCRYGG	1 cut(s)	1110
BstEII	GGTNACC	1 cut(s)	185
BstENI	CCTNNNNNAGG	1 cut(s)	2025
BstF5I	GGATG	6 cut(s)	763, 955, 1236, 1315, 1454, 1828
BstFNI	CGCG	1 cut(s)	2121
BstH2I	RGCGCY	2 cut(s)	329, 1549
BstHHI	GCGC	4 cut(s)	328, 1548, 1932, 2123
BstMAI	GTCTC	2 cut(s)	2078, 2094
BstMWI	GCNNNNNNNGC	9 cut(s)	110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090
BstNI	CCWGG	4 cut(s)	1609, 1718, 1749, 1870
BstNSI	RCATGY	5 cut(s)	512, 805, 1399, 1694, 2209
BstPI	GGTNACC	1 cut(s)	185
BstSCI	CCNGG	5 cut(s)	1200, 1607, 1716, 1747, 1868
BstSFI	CTRYAG	3 cut(s)	784, 1470, 1580
BstSLI	GKGCMC	1 cut(s)	1703
BstUI	CGCG	1 cut(s)	2121
BstV1I	GCAGC	7 cut(s)	315, 1637, 1882, 1904, 1934, 2135, 2231
BstV2I	GAAGAC	3 cut(s)	137, 1484, 2182
BstX2I	RGATCY	3 cut(s)	970, 1159, 1197
BstXI	CCANNNNNNTGG	1 cut(s)	735
BstYI	RGATCY	3 cut(s)	970, 1159, 1197
Bsu36I	CCTNAGG	1 cut(s)	212
BsuI	GTATCC	3 cut(s)	957, 976, 1258
BsuRI	GGCC	6 cut(s)	345, 352, 369, 594, 840, 1024
BtgI	CCRYGG	1 cut(s)	1110
BtgZI	GCGATG	3 cut(s)	354, 431, 2102
BtsCI	GGATG	6 cut(s)	763, 955, 1236, 1315, 1454, 1828
BtsI	GCAGTG	1 cut(s)	1467
BtsIMutI	CAGTG	6 cut(s)	187, 296, 433, 1467, 2181, 2240
CaiI	CAGNNNCTG	2 cut(s)	110, 1898
CfoI	GCGC	4 cut(s)	328, 1548, 1932, 2123
Cfr13I	GGNCC	5 cut(s)	368, 578, 839, 1023, 1319
CsiI	ACCWGGT	1 cut(s)	1868
Csp6I	GTAC	6 cut(s)	917, 1399, 1552, 1793, 1866, 2048
CspCI	CAANNNNNGTGG	2 cut(s)	1576, 1611
CviQI	GTAC	6 cut(s)	917, 1399, 1552, 1793, 1866, 2048
DdeI	CTNAG	8 cut(s)	126, 212, 299, 322, 438, 545, 1359, 1542
DraI	TTTAAA	1 cut(s)	1482
DriI	GACNNNNNGTC	1 cut(s)	509
EaeI	YGGCCR	2 cut(s)	343, 592
Eam1104I	CTCTTC	4 cut(s)	27, 111, 1014, 2118
Eam1105I	GACNNNNNGTC	1 cut(s)	509
EarI	CTCTTC	4 cut(s)	27, 111, 1014, 2118
Eco130I	CCWWGG	3 cut(s)	959, 1110, 2286
Eco24I	GRGCYC	1 cut(s)	2251
Eco31I	GGTCTC	2 cut(s)	2078, 2094
Eco32I	GATATC	1 cut(s)	943
Eco47I	GGWCC	2 cut(s)	578, 1319
Eco47III	AGCGCT	2 cut(s)	327, 1547
Eco57I	CTGAAG	3 cut(s)	135, 1176, 1242
Eco81I	CCTNAGG	1 cut(s)	212
Eco91I	GGTNACC	1 cut(s)	185
EcoNI	CCTNNNNNAGG	1 cut(s)	2025
EcoO109I	RGGNCCY	1 cut(s)	578
EcoO65I	GGTNACC	1 cut(s)	185
EcoRII	CCWGG	4 cut(s)	1607, 1716, 1747, 1868
EcoRV	GATATC	1 cut(s)	943
EcoT14I	CCWWGG	3 cut(s)	959, 1110, 2286
EcoT22I	ATGCAT	1 cut(s)	765
EcoT38I	GRGCYC	1 cut(s)	2251
ErhI	CCWWGG	3 cut(s)	959, 1110, 2286
FalI	AAGNNNNNCTT	4 cut(s)	960, 992, 1275, 1307
FaqI	GGGAC	4 cut(s)	516, 884, 1184, 2100
FauI	CCCGC	1 cut(s)	2077
FauNDI	CATATG	2 cut(s)	277, 1861
FbaI	TGATCA	2 cut(s)	634, 1038
Fnu4HI	GCNGC	8 cut(s)	138, 329, 1626, 1893, 1896, 1923, 2124, 2220
FokI	GGATG	6 cut(s)	750, 942, 1243, 1322, 1461, 1835
FriOI	GRGCYC	1 cut(s)	2251
Fsp4HI	GCNGC	8 cut(s)	138, 329, 1626, 1893, 1896, 1923, 2124, 2220
FspBI	CTAG	6 cut(s)	1194, 1269, 1296, 1332, 1880, 2045
FspI	TGCGCA	1 cut(s)	1931
GlaI	GCGC	4 cut(s)	327, 1547, 1931, 2122
GluI	GCNGC	8 cut(s)	138, 329, 1626, 1893, 1896, 1923, 2124, 2220
GsaI	CCCAGC	1 cut(s)	2173
GsuI	CTGGAG	2 cut(s)	228, 2205
HaeII	RGCGCY	2 cut(s)	329, 1549
HaeIII	GGCC	6 cut(s)	345, 352, 369, 594, 840, 1024
HapII	CCGG	2 cut(s)	1202, 1820
HhaI	GCGC	4 cut(s)	328, 1548, 1932, 2123
Hin1I	GRCGYC	1 cut(s)	794
Hin6I	GCGC	4 cut(s)	326, 1546, 1930, 2121
HinP1I	GCGC	4 cut(s)	326, 1546, 1930, 2121
HindIII	AAGCTT	2 cut(s)	8, 1284
HinfI	GANTC	5 cut(s)	35, 628, 685, 1556, 2187
HpaII	CCGG	2 cut(s)	1202, 1820
HphI	GGTGA	5 cut(s)	197, 1394, 1538, 1774, 2171
Hpy166II	GTNNAC	4 cut(s)	313, 1425, 1701, 2003
Hpy188I	TCNGA	9 cut(s)	439, 483, 572, 612, 1043, 1435, 1462, 1516, 1653
Hpy188III	TCNNGA	8 cut(s)	98, 632, 708, 1180, 1256, 1439, 2024, 2194
Hpy8I	GTNNAC	4 cut(s)	313, 1425, 1701, 2003
HpyCH4III	ACNGT	5 cut(s)	428, 1644, 1957, 1973, 2244
HpyCH4IV	ACGT	2 cut(s)	794, 1800
HpyF10VI	GCNNNNNNNGC	9 cut(s)	110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090
HpyF3I	CTNAG	8 cut(s)	126, 212, 299, 322, 438, 545, 1359, 1542
HpySE526I	ACGT	2 cut(s)	794, 1800
Hsp92I	GRCGYC	1 cut(s)	794
HspAI	GCGC	4 cut(s)	326, 1546, 1930, 2121
KpnI	GGTACC	1 cut(s)	1869
Ksp22I	TGATCA	2 cut(s)	634, 1038
LguI	GCTCTTC	1 cut(s)	111
LmnI	GCTCC	4 cut(s)	442, 1069, 1537, 1612
Lsp1109I	GCAGC	7 cut(s)	315, 1637, 1882, 1904, 1934, 2135, 2231
LweI	GCATC	8 cut(s)	91, 283, 427, 454, 772, 1195, 1227, 1497
MabI	ACCWGGT	1 cut(s)	1868
MaeI	CTAG	6 cut(s)	1194, 1269, 1296, 1332, 1880, 2045
MaeII	ACGT	2 cut(s)	794, 1800
MaeIII	GTNAC	5 cut(s)	185, 464, 646, 1060, 1382
MfeI	CAATTG	3 cut(s)	863, 904, 2266
MflI	RGATCY	3 cut(s)	970, 1159, 1197
MhlI	GDGCHC	2 cut(s)	1703, 2251
MlsI	TGGCCA	2 cut(s)	345, 594
MluNI	TGGCCA	2 cut(s)	345, 594
MlyI	GAGTC	2 cut(s)	44, 2196
MmeI	TCCRAC	1 cut(s)	1676
Mox20I	TGGCCA	2 cut(s)	345, 594
Mph1103I	ATGCAT	1 cut(s)	765
MroXI	GAANNNNTTC	1 cut(s)	224
MscI	TGGCCA	2 cut(s)	345, 594
MseI	TTAA	7 cut(s)	12, 174, 423, 699, 1481, 1646, 1734
MslI	CAYNNNNRTG	8 cut(s)	401, 483, 557, 642, 806, 927, 1109, 1689
Msp20I	TGGCCA	2 cut(s)	345, 594
MspA1I	CMGCKG	1 cut(s)	1895
MspCI	CTTAAG	1 cut(s)	11
MspI	CCGG	2 cut(s)	1202, 1820
MspR9I	CCNGG	5 cut(s)	1202, 1609, 1718, 1749, 1870
MunI	CAATTG	3 cut(s)	863, 904, 2266
MvaI	CCWGG	4 cut(s)	1609, 1718, 1749, 1870
MvnI	CGCG	1 cut(s)	2121
MwoI	GCNNNNNNNGC	9 cut(s)	110, 332, 362, 1151, 1283, 1292, 1401, 1625, 2090
NciI	CCSGG	1 cut(s)	1202
NcoI	CCATGG	1 cut(s)	1110
NdeI	CATATG	2 cut(s)	277, 1861
NlaIV	GGNNCC	4 cut(s)	1199, 1539, 1614, 1867
NmeAIII	GCCGAG	1 cut(s)	20
NmuCI	GTSAC	4 cut(s)	185, 646, 1060, 1382
NsbI	TGCGCA	1 cut(s)	1931
NsiI	ATGCAT	1 cut(s)	765
NspI	RCATGY	5 cut(s)	512, 805, 1399, 1694, 2209
PaeI	GCATGC	1 cut(s)	2209
PasI	CCCWGGG	1 cut(s)	1748
PciI	ACATGT	2 cut(s)	508, 1690
PciSI	GCTCTTC	1 cut(s)	111
PdmI	GAANNNNTTC	1 cut(s)	224
PfeI	GAWTC	3 cut(s)	628, 685, 1556
PfoI	TCCNGGA	1 cut(s)	1200
PkrI	GCNGC	8 cut(s)	139, 330, 1627, 1894, 1897, 1924, 2125, 2221
PleI	GAGTC	2 cut(s)	43, 2195
PpsI	GAGTC	2 cut(s)	43, 2195
PpuMI	RGGWCCY	1 cut(s)	578
PscI	ACATGT	2 cut(s)	508, 1690
PsiI	TTATAA	1 cut(s)	2135
Psp5II	RGGWCCY	1 cut(s)	578
Psp6I	CCWGG	4 cut(s)	1607, 1716, 1747, 1868
PspEI	GGTNACC	1 cut(s)	185
PspFI	CCCAGC	1 cut(s)	2169
PspGI	CCWGG	4 cut(s)	1607, 1716, 1747, 1868
PspN4I	GGNNCC	4 cut(s)	1199, 1539, 1614, 1867
PspPI	GGNCC	5 cut(s)	368, 578, 839, 1023, 1319
PspPPI	RGGWCCY	1 cut(s)	578
PsrI	GAACNNNNNNTAC	2 cut(s)	1085, 1117
PstI	CTGCAG	1 cut(s)	1474
PstNI	CAGNNNCTG	2 cut(s)	110, 1898
PsuI	RGATCY	3 cut(s)	970, 1159, 1197
PvuII	CAGCTG	1 cut(s)	1895
RsaI	GTAC	6 cut(s)	918, 1400, 1553, 1794, 1867, 2049
RsaNI	GTAC	6 cut(s)	917, 1399, 1552, 1793, 1866, 2048
RseI	CAYNNNNRTG	8 cut(s)	401, 483, 557, 642, 806, 927, 1109, 1689
SapI	GCTCTTC	1 cut(s)	111
SaqAI	TTAA	7 cut(s)	12, 174, 423, 699, 1481, 1646, 1734
SatI	GCNGC	8 cut(s)	138, 329, 1626, 1893, 1896, 1923, 2124, 2220
Sau96I	GGNCC	5 cut(s)	368, 578, 839, 1023, 1319
SchI	GAGTC	2 cut(s)	44, 2196
ScrFI	CCNGG	5 cut(s)	1202, 1609, 1718, 1749, 1870
SduI	GDGCHC	2 cut(s)	1703, 2251
SexAI	ACCWGGT	1 cut(s)	1868
SfaNI	GCATC	8 cut(s)	91, 283, 427, 454, 772, 1195, 1227, 1497
SfcI	CTRYAG	3 cut(s)	784, 1470, 1580
SinI	GGWCC	2 cut(s)	578, 1319
SmiMI	CAYNNNNRTG	8 cut(s)	401, 483, 557, 642, 806, 927, 1109, 1689
SmlI	CTYRAG	2 cut(s)	11, 1304
SmoI	CTYRAG	2 cut(s)	11, 1304
SpeI	ACTAGT	1 cut(s)	1268
SphI	GCATGC	1 cut(s)	2209
SsiI	CCGC	5 cut(s)	137, 163, 356, 1402, 2084
SspMI	CTAG	6 cut(s)	1194, 1269, 1296, 1332, 1880, 2045
StyD4I	CCNGG	5 cut(s)	1200, 1607, 1716, 1747, 1868
StyI	CCWWGG	3 cut(s)	959, 1110, 2286
TaaI	ACNGT	5 cut(s)	428, 1644, 1957, 1973, 2244
TaiI	ACGT	2 cut(s)	797, 1803
TaqI	TCGA	4 cut(s)	513, 683, 1502, 2025
TatI	WGTACW	1 cut(s)	2047
TauI	GCSGC	1 cut(s)	140
TfiI	GAWTC	3 cut(s)	628, 685, 1556
Tru1I	TTAA	7 cut(s)	12, 174, 423, 699, 1481, 1646, 1734
Tru9I	TTAA	7 cut(s)	12, 174, 423, 699, 1481, 1646, 1734
TscAI	CASTG	6 cut(s)	187, 303, 433, 1474, 2188, 2247
TseFI	GTSAC	4 cut(s)	185, 646, 1060, 1382
TseI	GCWGC	7 cut(s)	328, 1625, 1892, 1895, 1922, 2123, 2219
Tsp45I	GTSAC	4 cut(s)	185, 646, 1060, 1382
TspRI	CASTG	6 cut(s)	187, 303, 433, 1474, 2188, 2247
Vha464I	CTTAAG	1 cut(s)	11
VneI	GTGCAC	1 cut(s)	1699
VpaK11BI	GGWCC	2 cut(s)	578, 1319
XagI	CCTNNNNNAGG	1 cut(s)	2025
XapI	RAATTY	1 cut(s)	456
XceI	RCATGY	5 cut(s)	512, 805, 1399, 1694, 2209
XcmI	CCANNNNNNNNNTGG	2 cut(s)	774, 1117
XmnI	GAANNNNTTC	1 cut(s)	224
XspI	CTAG	6 cut(s)	1194, 1269, 1296, 1332, 1880, 2045
ZraI	GACGTC	1 cut(s)	795
Zsp2I	ATGCAT	1 cut(s)	765

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.