Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015872

Physical Location & Seq

Genomic Coordinates Reverse (-)

10009850 .. 10019494

Gene Structure 9645 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1136500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGGAGCTGTGGTGGTGGCAGCTGCGACGTGCTTTTGTTGTGGCAGCGGTGGTGGTAGTTATAACACAAGAAGGTTGTGTTCGCGGAGAAGATGTCACGTACGACGGCAGATCGCTCATCATTGATGGGGAAAGAAGACTTCTGTTTTCAGGTTCTATTCACTATCCTCGGAGTACACCTGAGATGTGGGGATCTTTGATTGGGAAAGCCAAGGAAGGAGGACTTGATGTAATTCAAACCTATGTATTTTGGAACCGTCACGAGCCCCAGCCGGGCCAGTACGATTTCAATAGAAGAAATGATCTAGTAAAATTTATAAAAGAAATCCAGGCACAAGGGCTTTATGCTTGCCTCAGGATCGGACCCTTCATTGAGAGTGAATGGACATATGGGGGACTTCCGTTTTGGCTGCATGACGTCCCAGGCATAGTTTACCGCTCAGATAATGAACCATTCAAGTTTTATATGCAAAACTTCACTACAAAAATAGTGAACTTGATGAAGTCAGAGGGTTTATATGCATCCGAAGGAGGTCCAATCATATTGTCGCAGATTGAGAATGAATATCAAAACGTAGAGGCAGCCTTCCATGAAAAAGGACCCTCCTACGTTCGTTGGGCTGCAAAAATGGCAGTTGACCTCCAGACTGGTGCACCATGGGTCATGTGCAAGCAAACCGATGCTCCTGATCCAGTGATTAATACATGCAATGGCATGAAATGTGGGCAAACTTTTGGAGGACCAAACTCACCCAATAAGCCCACTATGTGGACAGAGAATTGGACGTCATTCTATCAAGTGTATGGTGGAGAACCGTACATAAGGTCTGCTGAAGACATTGCATTTCATGTGGCTCTCTTTATAGCTAGAAATGGAAGCTACATCAACTATTACATGTATCACGGTGGAACTAATTTCGGAAGAACAGGCTCGGCATATGTCATAACAAGTTATTATGATCAAGCTCCTCTCGATGAATATGGTTTATTAAGGCAGCCCAAGTGGGGTCACCTAAAGGAGTTGCATGCTGCAATTAAAAGTTGCTCTACAACTTTACTAAACGGAACACCAACTAATTTTTCCTTAGGTCAACTTCAAGCGGCCTATGTCTTTAGCGAAGAAAGTGCAGGAAGATGTGTTGCATTTTTAATAAATAACGATGAAAAAAGTAACGCTACGGTTCAGTTCAGAAATGCATCTCATGAGCTGCTGCCAAAGTCAATCGCCATTTTATCTGACTGTGAAAAAGAAATTTTCAATACTGCAAGGGTTAATACAAAGCAAAATAAAAGAATCATAAGATCAACAAAAATATTTGATGCAATGGACAAATGGCAAAAGTTTACAGATGTCATCCCAAACTTTGAAAATACTTCACTACAATCAGATACATTACTCGAGCACATGAACGTGACAAAAGATGTATCGGATTATCTTTGGTATACTTTGAGTTTTCAACACAACATTTCTTGCGCTGAACCAGTACTTGTTGTAGAGTCTCTCGGACATGTAACACATGCTTTCATAAACAACATGTATATAGGAAATGGCCATGGCAGTCATGATACGAAGCAATTCGTCATGGAGAAACCAATTGGTTTAAACAACGGCACAAACAATATTTCTTTGCTAAGCGTAATGGTTGGACTTCCGGATTCAGGAGCTTTTCTAGAAAGAAGATTTGCTGGCTTAAGAAGAGTGGAAATTCAATGCAGTAAAAAAGAATTGTACAATTTTACTAATTACGATTGGGGATATCAGGTTGGACTACTTGGAGAAAAAATGCAAATATACAAAGAAGAAAATATAGACAATGTTGAATGGAGTGACGTTGGAGGCTCTTCAAATCAACCACTTACTTGGTACAAGACTGTTTTCGAGCCGCCGGTAGGAAATGATGCAGTCATCTTAAACCTAAGCTCAATGGGGAAGGGTGAAGCTTGGGTTAATGACCAAAGTATTGGTCGTTATTGGGTTTCATTTCTTACTTCTAAAGGAAAACCTTCCCAAACAATGTACCATGTTCCTCGATCATTCCTCAAAAACTCCGAAAACCTTTTAGTTTTGCTTGAAGAATATGGTGGAAACCCTCTTCACACTTCCTTAAACACCATTTCACTTTCCACTACGTATGAACCTATTTCTCACAACGAGCTTCGTTACAACAATTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009664 GO:0009791 GO:0009827 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0015925 GO:0016043 GO:0016787 GO:0016798 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0042545 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0048046 GO:0048316 GO:0048354 GO:0048359 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BetaGal_dom4_5 Gal_Lectin Glyco_hydro_35

Protein Analysis

725

Amino Acids

82.2

Weight (kDa)

5.88

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	38 - 344	9.4e-114	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	60 - 194	2.2e-06	Beta-galactosidase
GHD	PF17834	368 - 424	1.8e-21	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	619 - 685	5.5e-22	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015872)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63800
fragaria_vesca	FvH4_1g25140 FvH4_1g25140
malus_domestica	MD15G1136500.v1.1
prunus_persica	Prupe.4G279500_v2.0.a1
pyrus_communis	pycom15g12310
rosa_chinensis	RchiOBHm_Chr2g0122851
rosa_laevigata	RLG00000018718
rosa_roxburghii	Rroxscaffold_2G00121120
rosa_rugosa	Rorug02G0242700
rosa_samantha	Rh2AG301500 Rh2BG309000 Rh2CG287600 Rh2DG325600
rosa_wichuraiana	Rw2G023920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	62, 317
AatII	GACGTC	2 cut(s)	420, 788
AccB7I	CCANNNNNTGG	1 cut(s)	768
AccBSI	CCGCTC	1 cut(s)	438
AccI	GTMKAC	1 cut(s)	1442
AccII	CGCG	1 cut(s)	84
AccIII	TCCGGA	1 cut(s)	1651
AciI	CCGC	5 cut(s)	47, 84, 436, 1100, 1883
AclWI	GGATC	3 cut(s)	199, 365, 683
AcoI	YGGCCR	1 cut(s)	1549
AcsI	RAATTY	3 cut(s)	311, 1251, 1704
AcuI	CTGAAG	1 cut(s)	852
AcyI	GRCGYC	2 cut(s)	417, 785
AdeI	CACNNNGTG	1 cut(s)	768
AfaI	GTAC	8 cut(s)	101, 175, 281, 818, 1485, 1730, 1865, 2018
AfiI	CCNNNNNNNGG	5 cut(s)	272, 768, 1004, 1658, 1889
AflII	CTTAAG	1 cut(s)	1690
AflIII	ACRYGT	3 cut(s)	894, 1507, 1533
AjiI	CACGTC	1 cut(s)	29
AjnI	CCWGG	2 cut(s)	327, 421
AjuI	GAANNNNNNNTTGG	2 cut(s)	1063, 1095
Alw21I	GWGCWC	2 cut(s)	655, 1404
Alw26I	GTCTC	1 cut(s)	1503
Alw44I	GTGCAC	1 cut(s)	651
AlwI	GGATC	3 cut(s)	199, 365, 683
Ama87I	CYCGRG	1 cut(s)	1397
Aor13HI	TCCGGA	1 cut(s)	1651
AoxI	GGCC	3 cut(s)	274, 1101, 1549
ApaLI	GTGCAC	1 cut(s)	651
ApoI	RAATTY	3 cut(s)	311, 1251, 1704
ArsI	GACNNNNNNTTYG	4 cut(s)	387, 419, 1948, 1980
AseI	ATTAAT	1 cut(s)	699
Asp700I	GAANNNNTTC	2 cut(s)	1574, 2002
AspLEI	GCGC	1 cut(s)	1475
AspS9I	GGNCC	5 cut(s)	274, 362, 533, 599, 740
AsuC2I	CCSGG	1 cut(s)	273
AsuHPI	GGTGA	3 cut(s)	741, 1001, 1946
AvaI	CYCGRG	1 cut(s)	1397
AvaII	GGWCC	4 cut(s)	362, 533, 599, 740
AxyI	CCTNAGG	2 cut(s)	353, 1084
BaeGI	GKGCMC	1 cut(s)	655
BalI	TGGCCA	1 cut(s)	1551
BanII	GRGCYC	1 cut(s)	267
BauI	CACGAG	1 cut(s)	260
BbsI	GAAGAC	2 cut(s)	142, 840
Bbv12I	GWGCWC	2 cut(s)	655, 1404
BccI	CCATC	1 cut(s)	119
BceAI	ACGGC	2 cut(s)	121, 1624
BcgI	CGANNNNNNTGC	2 cut(s)	1106, 1140
BciT130I	CCWGG	2 cut(s)	329, 423
BclI	TGATCA	1 cut(s)	958
BcnI	CCSGG	1 cut(s)	273
BcoDI	GTCTC	1 cut(s)	1503
BfaI	CTAG	3 cut(s)	305, 867, 1670
BfrI	CTTAAG	1 cut(s)	1690
BlpI	GCTNAGC	1 cut(s)	1631
BmcAI	AGTACT	1 cut(s)	1485
Bme1390I	CCNGG	3 cut(s)	273, 329, 423
Bme18I	GGWCC	4 cut(s)	362, 533, 599, 740
BmeT110I	CYCGRG	1 cut(s)	1397
BmgBI	CACGTC	1 cut(s)	29
BmgT120I	GGNCC	5 cut(s)	274, 362, 533, 599, 740
BmiI	GGNNCC	3 cut(s)	254, 364, 601
BmrFI	CCNGG	3 cut(s)	273, 329, 423
BmsI	GCATC	5 cut(s)	530, 670, 1205, 1309, 1888
BpiI	GAAGAC	2 cut(s)	142, 840
BpmI	CTGGAG	1 cut(s)	626
Bpu10I	CCTNAGC	1 cut(s)	1916
Bpu1102I	GCTNAGC	1 cut(s)	1631
BpuMI	CCSGG	1 cut(s)	273
BsaAI	YACGTR	2 cut(s)	99, 2130
BsaHI	GRCGYC	2 cut(s)	417, 785
BsaJI	CCNNGG	5 cut(s)	167, 210, 421, 656, 1552
BsaWI	WCCGGW	1 cut(s)	1651
BsaXI	ACNNNNNCTCC	5 cut(s)	26, 78, 108, 667, 697
Bsc4I	CCNNNNNNNGG	5 cut(s)	272, 768, 1004, 1658, 1889
Bse118I	RCCGGY	1 cut(s)	1885
Bse1I	ACTGG	4 cut(s)	277, 652, 692, 1481
Bse21I	CCTNAGG	2 cut(s)	353, 1084
Bse3DI	GCAATG	3 cut(s)	715, 837, 1329
BseAI	TCCGGA	1 cut(s)	1651
BseBI	CCWGG	2 cut(s)	329, 423
BseDI	CCNNGG	5 cut(s)	167, 210, 421, 656, 1552
BseGI	GGATG	2 cut(s)	521, 1353
BseLI	CCNNNNNNNGG	5 cut(s)	272, 768, 1004, 1658, 1889
BseMI	GCAATG	3 cut(s)	715, 837, 1329
BseMII	CTCAG	3 cut(s)	171, 367, 453
BseNI	ACTGG	4 cut(s)	277, 652, 692, 1481
BseRI	GAGGAG	1 cut(s)	957
BseSI	GKGCMC	1 cut(s)	655
BseYI	CCCAGC	1 cut(s)	267
BsgI	GTGCAG	1 cut(s)	1146
Bsh1236I	CGCG	1 cut(s)	84
BshFI	GGCC	3 cut(s)	276, 1103, 1551
BsiHKAI	GWGCWC	2 cut(s)	655, 1404
BsiHKCI	CYCGRG	1 cut(s)	1397
BsiSI	CCGG	3 cut(s)	272, 1652, 1886
BsiWI	CGTACG	1 cut(s)	99
BslFI	GGGAC	2 cut(s)	404, 408
BslI	CCNNNNNNNGG	5 cut(s)	272, 768, 1004, 1658, 1889
BsmAI	GTCTC	1 cut(s)	1503
BsmFI	GGGAC	2 cut(s)	404, 408
BsnI	GGCC	3 cut(s)	276, 1103, 1551
BsoBI	CYCGRG	1 cut(s)	1397
Bsp1286I	GDGCHC	3 cut(s)	267, 655, 1404
Bsp13I	TCCGGA	1 cut(s)	1651
Bsp1407I	TGTACA	1 cut(s)	1728
Bsp143I	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
Bsp1720I	GCTNAGC	1 cut(s)	1631
Bsp19I	CCATGG	2 cut(s)	656, 1552
BspACI	CCGC	5 cut(s)	47, 84, 436, 1100, 1883
BspANI	GGCC	3 cut(s)	276, 1103, 1551
BspCNI	CTCAG	3 cut(s)	172, 366, 452
BspEI	TCCGGA	1 cut(s)	1651
BspFNI	CGCG	1 cut(s)	84
BspHI	TCATGA	2 cut(s)	1201, 1561
BspLI	GGNNCC	3 cut(s)	254, 364, 601
BspPI	GGATC	3 cut(s)	199, 365, 683
BspQI	GCTCTTC	1 cut(s)	1846
BspTI	CTTAAG	1 cut(s)	1690
BsrBI	CCGCTC	1 cut(s)	438
BsrDI	GCAATG	3 cut(s)	715, 837, 1329
BsrFI	RCCGGY	1 cut(s)	1885
BsrGI	TGTACA	1 cut(s)	1728
BsrI	ACTGG	4 cut(s)	277, 652, 692, 1481
BssAI	RCCGGY	1 cut(s)	1885
BssECI	CCNNGG	5 cut(s)	167, 210, 421, 656, 1552
BssMI	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
BssNAI	GTATAC	1 cut(s)	1443
BssNI	GRCGYC	2 cut(s)	417, 785
BssSI	CACGAG	1 cut(s)	260
BssT1I	CCWWGG	3 cut(s)	210, 656, 1552
Bst1107I	GTATAC	1 cut(s)	1443
Bst2BI	CACGAG	1 cut(s)	260
Bst2UI	CCWGG	2 cut(s)	329, 423
Bst4CI	ACNGT	6 cut(s)	257, 816, 905, 1180, 1241, 1873
Bst6I	CTCTTC	3 cut(s)	1690, 1846, 2097
BstACI	GRCGYC	2 cut(s)	417, 785
BstAFI	CTTAAG	1 cut(s)	1690
BstAUI	TGTACA	1 cut(s)	1728
BstBAI	YACGTR	2 cut(s)	99, 2130
BstC8I	GCNNGC	4 cut(s)	349, 671, 1026, 1687
BstDEI	CTNAG	6 cut(s)	180, 353, 439, 1084, 1631, 1916
BstDSI	CCRYGG	2 cut(s)	656, 1552
BstEII	GGTNACC	1 cut(s)	1007
BstF5I	GGATG	2 cut(s)	521, 1353
BstFNI	CGCG	1 cut(s)	84
BstHHI	GCGC	1 cut(s)	1475
BstKTI	GATC	8 cut(s)	113, 194, 304, 360, 691, 961, 1304, 2033
BstMAI	GTCTC	1 cut(s)	1503
BstMBI	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
BstMWI	GCNNNNNNNGC	1 cut(s)	629
BstNI	CCWGG	2 cut(s)	329, 423
BstNSI	RCATGY	6 cut(s)	708, 898, 1028, 1511, 1520, 1537
BstPI	GGTNACC	1 cut(s)	1007
BstSCI	CCNGG	3 cut(s)	271, 327, 421
BstSLI	GKGCMC	1 cut(s)	655
BstSNI	TACGTA	1 cut(s)	2130
BstUI	CGCG	1 cut(s)	84
BstV2I	GAAGAC	2 cut(s)	142, 840
BstX2I	RGATCY	1 cut(s)	191
BstXI	CCANNNNNNTGG	2 cut(s)	1860, 1961
BstYI	RGATCY	1 cut(s)	191
BstZ17I	GTATAC	1 cut(s)	1443
Bsu36I	CCTNAGG	2 cut(s)	353, 1084
BsuRI	GGCC	3 cut(s)	276, 1103, 1551
BtgI	CCRYGG	2 cut(s)	656, 1552
BtrI	CACGTC	1 cut(s)	29
BtsCI	GGATG	2 cut(s)	521, 1353
BtsIMutI	CAGTG	1 cut(s)	699
Cac8I	GCNNGC	4 cut(s)	349, 671, 1026, 1687
CciI	TCATGA	2 cut(s)	1201, 1561
CfoI	GCGC	1 cut(s)	1475
Cfr10I	RCCGGY	1 cut(s)	1885
Cfr13I	GGNCC	5 cut(s)	274, 362, 533, 599, 740
Csp6I	GTAC	8 cut(s)	100, 174, 280, 817, 1484, 1729, 1864, 2017
CviQI	GTAC	8 cut(s)	100, 174, 280, 817, 1484, 1729, 1864, 2017
DdeI	CTNAG	6 cut(s)	180, 353, 439, 1084, 1631, 1916
DpnI	GATC	8 cut(s)	112, 193, 303, 359, 690, 960, 1303, 2032
DpnII	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
DraI	TTTAAA	1 cut(s)	1602
DraIII	CACNNNGTG	1 cut(s)	768
EaeI	YGGCCR	1 cut(s)	1549
Eam1104I	CTCTTC	3 cut(s)	1690, 1846, 2097
EarI	CTCTTC	3 cut(s)	1690, 1846, 2097
Eco105I	TACGTA	1 cut(s)	2130
Eco130I	CCWWGG	3 cut(s)	210, 656, 1552
Eco24I	GRGCYC	1 cut(s)	267
Eco32I	GATATC	1 cut(s)	1757
Eco47I	GGWCC	4 cut(s)	362, 533, 599, 740
Eco57I	CTGAAG	1 cut(s)	852
Eco81I	CCTNAGG	2 cut(s)	353, 1084
Eco88I	CYCGRG	1 cut(s)	1397
Eco91I	GGTNACC	1 cut(s)	1007
EcoO109I	RGGNCCY	1 cut(s)	599
EcoO65I	GGTNACC	1 cut(s)	1007
EcoRII	CCWGG	2 cut(s)	327, 421
EcoRV	GATATC	1 cut(s)	1757
EcoT14I	CCWWGG	3 cut(s)	210, 656, 1552
EcoT22I	ATGCAT	2 cut(s)	523, 1198
EcoT38I	GRGCYC	1 cut(s)	267
ErhI	CCWWGG	3 cut(s)	210, 656, 1552
FalI	AAGNNNNNCTT	2 cut(s)	207, 239
FaqI	GGGAC	2 cut(s)	404, 408
FauNDI	CATATG	2 cut(s)	388, 937
FbaI	TGATCA	1 cut(s)	958
FblI	GTMKAC	1 cut(s)	1442
FokI	GGATG	2 cut(s)	508, 1340
FriOI	GRGCYC	1 cut(s)	267
FspBI	CTAG	3 cut(s)	305, 867, 1670
GlaI	GCGC	1 cut(s)	1474
GsaI	CCCAGC	1 cut(s)	271
GsuI	CTGGAG	1 cut(s)	626
HaeIII	GGCC	3 cut(s)	276, 1103, 1551
HapII	CCGG	3 cut(s)	272, 1652, 1886
HhaI	GCGC	1 cut(s)	1475
Hin1I	GRCGYC	2 cut(s)	417, 785
Hin6I	GCGC	1 cut(s)	1473
HinP1I	GCGC	1 cut(s)	1473
HincII	GTYRAC	2 cut(s)	637, 1091
HindII	GTYRAC	2 cut(s)	637, 1091
HindIII	AAGCTT	1 cut(s)	1938
HinfI	GANTC	3 cut(s)	1293, 1496, 1655
HpaII	CCGG	3 cut(s)	272, 1652, 1886
HphI	GGTGA	3 cut(s)	741, 1001, 1946
Hpy166II	GTNNAC	9 cut(s)	176, 433, 493, 637, 653, 771, 1091, 1344, 1443
Hpy8I	GTNNAC	9 cut(s)	176, 433, 493, 637, 653, 771, 1091, 1344, 1443
Hpy99I	CGWCG	2 cut(s)	30, 107
HpyAV	CCTTC	7 cut(s)	65, 209, 376, 521, 595, 1924, 2013
HpyCH4III	ACNGT	6 cut(s)	257, 816, 905, 1180, 1241, 1873
HpyCH4IV	ACGT	9 cut(s)	28, 98, 417, 573, 609, 785, 1410, 1830, 2129
HpyF10VI	GCNNNNNNNGC	1 cut(s)	629
HpyF3I	CTNAG	6 cut(s)	180, 353, 439, 1084, 1631, 1916
HpySE526I	ACGT	9 cut(s)	28, 98, 417, 573, 609, 785, 1410, 1830, 2129
Hsp92I	GRCGYC	2 cut(s)	417, 785
HspAI	GCGC	1 cut(s)	1473
Kpn2I	TCCGGA	1 cut(s)	1651
Ksp22I	TGATCA	1 cut(s)	958
Kzo9I	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
LguI	GCTCTTC	1 cut(s)	1846
LmnI	GCTCC	4 cut(s)	4, 688, 970, 1661
LweI	GCATC	5 cut(s)	530, 670, 1205, 1309, 1888
MaeI	CTAG	3 cut(s)	305, 867, 1670
MaeII	ACGT	9 cut(s)	28, 98, 417, 573, 609, 785, 1410, 1830, 2129
MaeIII	GTNAC	8 cut(s)	94, 257, 1007, 1169, 1411, 1510, 1826, 2159
MalI	GATC	8 cut(s)	112, 193, 303, 359, 690, 960, 1303, 2032
MbiI	CCGCTC	1 cut(s)	438
MboI	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
MfeI	CAATTG	1 cut(s)	1593
MflI	RGATCY	1 cut(s)	191
MhlI	GDGCHC	3 cut(s)	267, 655, 1404
MlsI	TGGCCA	1 cut(s)	1551
MluNI	TGGCCA	1 cut(s)	1551
MlyI	GAGTC	1 cut(s)	1505
MmeI	TCCRAC	3 cut(s)	1624, 1744, 1813
Mox20I	TGGCCA	1 cut(s)	1551
Mph1103I	ATGCAT	2 cut(s)	523, 1198
MroI	TCCGGA	1 cut(s)	1651
MroXI	GAANNNNTTC	2 cut(s)	1574, 2002
MscI	TGGCCA	1 cut(s)	1551
MslI	CAYNNNNRTG	1 cut(s)	1409
Msp20I	TGGCCA	1 cut(s)	1551
MspA1I	CMGCKG	2 cut(s)	22, 47
MspCI	CTTAAG	1 cut(s)	1690
MspI	CCGG	3 cut(s)	272, 1652, 1886
MspR9I	CCNGG	3 cut(s)	273, 329, 423
MssI	GTTTAAAC	1 cut(s)	1602
MunI	CAATTG	1 cut(s)	1593
MvaI	CCWGG	2 cut(s)	329, 423
MvnI	CGCG	1 cut(s)	84
MwoI	GCNNNNNNNGC	1 cut(s)	629
NciI	CCSGG	1 cut(s)	273
NcoI	CCATGG	2 cut(s)	656, 1552
NdeI	CATATG	2 cut(s)	388, 937
NdeII	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
NlaIV	GGNNCC	3 cut(s)	254, 364, 601
NmeAIII	GCCGAG	1 cut(s)	911
NmuCI	GTSAC	5 cut(s)	94, 257, 1007, 1411, 1826
NsiI	ATGCAT	2 cut(s)	523, 1198
NspI	RCATGY	6 cut(s)	708, 898, 1028, 1511, 1520, 1537
PaeI	GCATGC	1 cut(s)	1028
PaeR7I	CTCGAG	1 cut(s)	1397
PagI	TCATGA	2 cut(s)	1201, 1561
PciI	ACATGT	3 cut(s)	894, 1507, 1533
PciSI	GCTCTTC	1 cut(s)	1846
PdmI	GAANNNNTTC	2 cut(s)	1574, 2002
PfeI	GAWTC	2 cut(s)	1293, 1655
Pfl23II	CGTACG	1 cut(s)	99
PflMI	CCANNNNNTGG	1 cut(s)	768
PleI	GAGTC	1 cut(s)	1504
PmeI	GTTTAAAC	1 cut(s)	1602
PpsI	GAGTC	1 cut(s)	1504
Ppu21I	YACGTR	2 cut(s)	99, 2130
PpuMI	RGGWCCY	1 cut(s)	599
PscI	ACATGT	3 cut(s)	894, 1507, 1533
PshBI	ATTAAT	1 cut(s)	699
PsiI	TTATAA	2 cut(s)	62, 317
Psp5II	RGGWCCY	1 cut(s)	599
Psp6I	CCWGG	2 cut(s)	327, 421
PspEI	GGTNACC	1 cut(s)	1007
PspFI	CCCAGC	1 cut(s)	267
PspGI	CCWGG	2 cut(s)	327, 421
PspLI	CGTACG	1 cut(s)	99
PspN4I	GGNNCC	3 cut(s)	254, 364, 601
PspPI	GGNCC	5 cut(s)	274, 362, 533, 599, 740
PspPPI	RGGWCCY	1 cut(s)	599
PspXI	VCTCGAGB	1 cut(s)	1397
PsuI	RGATCY	1 cut(s)	191
PvuII	CAGCTG	1 cut(s)	22
RsaI	GTAC	8 cut(s)	101, 175, 281, 818, 1485, 1730, 1865, 2018
RsaNI	GTAC	8 cut(s)	100, 174, 280, 817, 1484, 1729, 1864, 2017
RseI	CAYNNNNRTG	1 cut(s)	1409
SapI	GCTCTTC	1 cut(s)	1846
Sau3AI	GATC	8 cut(s)	110, 191, 301, 357, 688, 958, 1301, 2030
Sau96I	GGNCC	5 cut(s)	274, 362, 533, 599, 740
ScaI	AGTACT	1 cut(s)	1485
SchI	GAGTC	1 cut(s)	1505
ScrFI	CCNGG	3 cut(s)	273, 329, 423
SduI	GDGCHC	3 cut(s)	267, 655, 1404
SfaNI	GCATC	5 cut(s)	530, 670, 1205, 1309, 1888
Sfr274I	CTCGAG	1 cut(s)	1397
SinI	GGWCC	4 cut(s)	362, 533, 599, 740
SlaI	CTCGAG	1 cut(s)	1397
SmiMI	CAYNNNNRTG	1 cut(s)	1409
SmlI	CTYRAG	2 cut(s)	1397, 1690
SmoI	CTYRAG	2 cut(s)	1397, 1690
SnaBI	TACGTA	1 cut(s)	2130
SphI	GCATGC	1 cut(s)	1028
SsiI	CCGC	5 cut(s)	47, 84, 436, 1100, 1883
SspI	AATATT	2 cut(s)	1314, 1621
SspMI	CTAG	3 cut(s)	305, 867, 1670
StyD4I	CCNGG	3 cut(s)	271, 327, 421
StyI	CCWWGG	3 cut(s)	210, 656, 1552
TaaI	ACNGT	6 cut(s)	257, 816, 905, 1180, 1241, 1873
TaiI	ACGT	9 cut(s)	31, 101, 420, 576, 612, 788, 1413, 1833, 2132
TaqI	TCGA	4 cut(s)	972, 1398, 1878, 2029
TatI	WGTACW	3 cut(s)	173, 1483, 1728
TauI	GCSGC	2 cut(s)	1103, 1885
TfiI	GAWTC	2 cut(s)	1293, 1655
TscAI	CASTG	1 cut(s)	699
TseFI	GTSAC	5 cut(s)	94, 257, 1007, 1411, 1826
Tsp45I	GTSAC	5 cut(s)	94, 257, 1007, 1411, 1826
TspGWI	ACGGA	2 cut(s)	390, 1077
TspRI	CASTG	1 cut(s)	699
Van91I	CCANNNNNTGG	1 cut(s)	768
Vha464I	CTTAAG	1 cut(s)	1690
VneI	GTGCAC	1 cut(s)	651
VpaK11BI	GGWCC	4 cut(s)	362, 533, 599, 740
VspI	ATTAAT	1 cut(s)	699
XapI	RAATTY	3 cut(s)	311, 1251, 1704
XbaI	TCTAGA	1 cut(s)	1669
XceI	RCATGY	6 cut(s)	708, 898, 1028, 1511, 1520, 1537
XhoI	CTCGAG	1 cut(s)	1397
XmiI	GTMKAC	1 cut(s)	1442
XmnI	GAANNNNTTC	2 cut(s)	1574, 2002
XspI	CTAG	3 cut(s)	305, 867, 1670
ZraI	GACGTC	2 cut(s)	418, 786
ZrmI	AGTACT	1 cut(s)	1485
Zsp2I	ATGCAT	2 cut(s)	523, 1198

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.