RchiOBHm_Chr2g0122851

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015872
Physical Location & Seq
Reverse (-)
35910405 .. 35916570
6166 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49521

Sequence Viewer

Length: 2208 bp
ATGGAATGGCGCTGGCTACAGTTTTGGGTTATGGTGGTGGTGGTTCTGATTACTGATGAGAGGGTTTCTGTTAGTGGAGGAAATGTGACCTACGACGGAAGATCTCTCATCATCGACGGCCAAAGAAAGATTCTTTTTTCAGGTTCTATTCACTATCCTCGGAGTACACCTGAGATGTGGCCATCTCTGATCGGAAAAGCCAAGGAAGGAGGACTTGATGTGATTCAAACCTATGTGTTTTGGAACCTTCACGAGCCTCAACCGGGTCAGTACGATTTCAGTGGAAGATATGATCTAGTAAAGTTCATAAAGGAAATCCAAGCACAAGGGCTTTATGCTTGCCTTAGGATTGGACCATTCATTGAGAGTGAATGGACATATGGGGGTTTTCCATTTTGGCTGCATGATGTCCCAGGCATAGTTTACCGCACAGATAATGAGCCCTTTAAGGCATGCACTCTTCCTATAACTAATTATATATTTTACATGCAAAATTTTACTACAAAAATTGTGAACTTGATGAAATCAGAGGGATTATATGCTTCACAAGGAGGTCCAATCATACTCTCGCAGATTGAAAATGAATATCAAAATGTGGAAGCAGCATTCCACGAGAAAGGACCTGCCTACGTTCGATGGGCTGCAAAAATGGCAGTCGACCTCCAAACTGGTGTGCCATGGGTGATGTGCAAGCAAACTGATGCTCCTGATCCAGTGATCAATACCTGCAATGGAATGCAATGTGGGAAGACTTTTGGAGGACCAAACTCACCTAATAAACCCTCTATGTGGACAGAGAATTGGACGTCATTCTATCAAGTGTATGGCGGAGAACCATACATAAGATCTGCTGAAAATATTGCATTTCACGTTGCGCTTTTTATAGCTCGGCTGAATGGAAGCTATGTAAACTACTACATGTATCATGGTGGAACTAACTTTGGAAGAACAAGCTCTGCATATGTCATAACAGGTTATTATGATCAAGCTCCGCTAGATGAATATGGTTTGTTAAGGCAACCCAAATGGGGTCATCTTAAGCAATTGCATGCTGCATTTAGAAACTGCTCGACAACTTTACTGCAGGGAATGAGAACTAATTTTTCTTTAGGTCAACTTCAAGAGGCCTATGTCTTTCAAGAAGAAAGTGCTGGAAGATGTGTTGCATTTCTAGTAAATAACGATGAAAAAACTAATGCTACTGTTCAATTTCGAAATATATCATACGACCTGATGCCAATGTCAATCAGCATTCTACCAGACTGTGAAAATGTGATTTTCAACACTGCAAAGGTGAATACAAGCTCTAACAAAAGAATTACAAGATCAGCTCAAATCTTTGATTCAGTGGATAGTTGGGAGGGGTCTAAAGATGTCATCCAAAATTTTGAACCTACAATATCTCTACAATCGGATACACTACTTGAGCACATGAACGTGACCAAAGACAAATCTGATTATCTTTGGTATACTATGAGATTTCAGCACAACATTTCTTGCACTGAACCAGTACTTCGTGTAGAGTCCTTCGGACATGTAACACATGCTTTCGTGAATGGCATAAACACAGGAAATGCACATGGCAGTCATGATACCAAACAATTCATTATGGAGATTCCAGTTCTGTTAAACGATGGAAGTAACAATATTTCTTTGCTAAGCGTTATGGTTGGACTACCGGACTCGGGTGCTTTTCTTGAAAGCAAATTTGCTGGCTTGAAAAGAGTTGAAATTCACTGCAACGAAACCGATCAATTTTACAATTTTACTAACTATGCATGGGGATACCAGGTTGGGTTACTTGGAGAAAAGTTGCAAATATATAGAGAAGAAAATCTGGACAACATTGAATGGAACAATATTGAAGGCTATTTTAATCAATCACTGACTTGGTACATGACATTTTTCGATGCACCTGTGGGAAATGATCCAGTCGGCTTAAACCTTAGCTCAATGGCAAAGGGCGAAGCTTGGATTAATGGACAAAGTATTGGTCGATATTGGGTTTCATTCCTTACTTCGAAAGGCCAACCTTCACAATCGATGTACCACGTTCCTCGATCATTCTTAAAAGATTCTGAAAACCTTTTAGTTTTGCTCGAAGAAGTTGGTGGAAACCCTCTTCACATTTCTTTAGATACTATTTCACTTTCCTCTCTATCTGAAAGTACTTCTTATCATCAGATCCCATCAATAAGTTTAGAGTGA

Protein Analysis

735

Amino Acids

83.31

Weight (kDa)

5.35

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 35 - 352 1.3e-111 Glycosyl hydrolases family 35
Glyco_hydro_42 PF02449 57 - 213 1.8e-08 Beta-galactosidase
GHD PF17834 375 - 432 2.3e-24 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 629 - 695 8.2e-23 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015872)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63800
fragaria_vesca FvH4_1g25140 FvH4_1g25140
malus_domestica MD15G1136500.v1.1
prunus_persica Prupe.4G279500_v2.0.a1
pyrus_communis pycom15g12310
rosa_chinensis RchiOBHm_Chr2g0122851
rosa_laevigata RLG00000018718
rosa_roxburghii Rroxscaffold_2G00121120
rosa_rugosa Rorug02G0242700
rosa_samantha Rh2AG301500 Rh2BG309000 Rh2CG287600 Rh2DG325600
rosa_wichuraiana Rw2G023920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 809
Acc36I ACCTGC 2 cut(s) 631, 734
AccI GTMKAC 2 cut(s) 657, 1469
AciI CCGC 3 cut(s) 427, 828, 992
AclWI GGATC 3 cut(s) 704, 1922, 2179
AcoI YGGCCR 2 cut(s) 118, 179
AcsI RAATTY 4 cut(s) 493, 1384, 1706, 1731
AcyI GRCGYC 1 cut(s) 806
AfaI GTAC 6 cut(s) 166, 272, 1512, 1895, 2048, 2170
AfiI CCNNNNNNNGG 4 cut(s) 263, 789, 1028, 1685
AflII CTTAAG 1 cut(s) 1037
AflIII ACRYGT 2 cut(s) 918, 1534
AjnI CCWGG 2 cut(s) 412, 1788
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AluBI AGCT 8 cut(s) 887, 903, 954, 989, 1305, 1331, 1950, 1970
AluI AGCT 8 cut(s) 887, 903, 954, 989, 1305, 1331, 1950, 1970
Alw21I GWGCWC 1 cut(s) 1431
AlwI GGATC 3 cut(s) 704, 1922, 2179
Ama87I CYCGRG 1 cut(s) 1684
AoxI GGCC 4 cut(s) 118, 179, 1125, 2026
ApeKI GCWGC 4 cut(s) 400, 602, 641, 1052
ApoI RAATTY 4 cut(s) 493, 1384, 1706, 1731
ArsI GACNNNNNNTTYG 2 cut(s) 1978, 2010
AseI ATTAAT 1 cut(s) 1977
AspLEI GCGC 2 cut(s) 12, 877
AspS9I GGNCC 4 cut(s) 353, 554, 620, 761
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 3 cut(s) 694, 762, 1306
AsuII TTCGAA 2 cut(s) 1213, 2021
AvaI CYCGRG 1 cut(s) 1684
AvaII GGWCC 4 cut(s) 353, 554, 620, 761
AxyI CCTNAGG 1 cut(s) 344
BalI TGGCCA 1 cut(s) 181
BanII GRGCYC 1 cut(s) 444
BauI CACGAG 2 cut(s) 251, 611
BbsI GAAGAC 1 cut(s) 755
Bbv12I GWGCWC 1 cut(s) 1431
BbvI GCAGC 4 cut(s) 387, 614, 628, 1039
BccI CCATC 4 cut(s) 190, 630, 1628, 2197
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 2 cut(s) 414, 1790
BciVI GTATCC 2 cut(s) 1408, 1778
BclI TGATCA 2 cut(s) 717, 982
BcnI CCSGG 1 cut(s) 264
BfaI CTAG 3 cut(s) 296, 995, 1172
BfmI CTRYAG 2 cut(s) 17, 1082
BfoI RGCGCY 1 cut(s) 13
BfrI CTTAAG 1 cut(s) 1037
BfuAI ACCTGC 2 cut(s) 631, 734
BfuI GTATCC 2 cut(s) 1408, 1778
BglII AGATCT 2 cut(s) 101, 845
BisI GCNGC 4 cut(s) 401, 603, 642, 1053
BlpI GCTNAGC 1 cut(s) 1658
BlsI GCNGC 4 cut(s) 402, 604, 643, 1054
BmcAI AGTACT 2 cut(s) 1512, 2170
Bme1390I CCNGG 3 cut(s) 264, 414, 1790
Bme18I GGWCC 4 cut(s) 353, 554, 620, 761
BmeT110I CYCGRG 1 cut(s) 1684
BmgT120I GGNCC 4 cut(s) 353, 554, 620, 761
BmiI GGNNCC 1 cut(s) 245
BmrFI CCNGG 3 cut(s) 264, 414, 1790
BmsI GCATC 3 cut(s) 691, 1224, 1900
BpiI GAAGAC 1 cut(s) 755
Bpu10I CCTNAGC 1 cut(s) 1946
Bpu1102I GCTNAGC 1 cut(s) 1658
Bpu14I TTCGAA 2 cut(s) 1213, 2021
BpuEI CTTGAG 1 cut(s) 1445
BpuMI CCSGG 1 cut(s) 264
Bsa29I ATCGAT 1 cut(s) 2042
BsaHI GRCGYC 1 cut(s) 806
BsaJI CCNNGG 4 cut(s) 158, 201, 412, 677
BsaWI WCCGGW 1 cut(s) 1678
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 688, 718
Bsc4I CCNNNNNNNGG 4 cut(s) 263, 789, 1028, 1685
Bse1I ACTGG 5 cut(s) 673, 713, 1508, 1619, 1931
Bse21I CCTNAGG 1 cut(s) 344
Bse3DI GCAATG 2 cut(s) 736, 746
BseBI CCWGG 2 cut(s) 414, 1790
BseCI ATCGAT 1 cut(s) 2042
BseDI CCNNGG 4 cut(s) 158, 201, 412, 677
BseGI GGATG 1 cut(s) 1377
BseLI CCNNNNNNNGG 4 cut(s) 263, 789, 1028, 1685
BseMI GCAATG 2 cut(s) 736, 746
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 5 cut(s) 673, 713, 1508, 1619, 1931
BseXI GCAGC 4 cut(s) 387, 614, 628, 1039
BshFI GGCC 4 cut(s) 120, 181, 1127, 2028
BshVI ATCGAT 1 cut(s) 2042
BsiHKAI GWGCWC 1 cut(s) 1431
BsiHKCI CYCGRG 1 cut(s) 1684
BsiSI CCGG 2 cut(s) 263, 1679
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 4 cut(s) 263, 789, 1028, 1685
BsmFI GGGAC 1 cut(s) 395
BsmI GAATGC 3 cut(s) 605, 741, 1251
BsnI GGCC 4 cut(s) 120, 181, 1127, 2028
BsoBI CYCGRG 1 cut(s) 1684
Bsp119I TTCGAA 2 cut(s) 1213, 2021
Bsp1286I GDGCHC 2 cut(s) 444, 1431
Bsp1720I GCTNAGC 1 cut(s) 1658
Bsp19I CCATGG 1 cut(s) 677
BspACI CCGC 3 cut(s) 427, 828, 992
BspANI GGCC 4 cut(s) 120, 181, 1127, 2028
BspCNI CTCAG 1 cut(s) 163
BspDI ATCGAT 1 cut(s) 2042
BspHI TCATGA 1 cut(s) 1588
BspLI GGNNCC 1 cut(s) 245
BspMAI CTGCAG 1 cut(s) 1086
BspMI ACCTGC 2 cut(s) 631, 734
BspPI GGATC 3 cut(s) 704, 1922, 2179
BspT104I TTCGAA 2 cut(s) 1213, 2021
BspTI CTTAAG 1 cut(s) 1037
BsrDI GCAATG 2 cut(s) 736, 746
BsrI ACTGG 5 cut(s) 673, 713, 1508, 1619, 1931
BssECI CCNNGG 4 cut(s) 158, 201, 412, 677
BssNAI GTATAC 1 cut(s) 1470
BssNI GRCGYC 1 cut(s) 806
BssSI CACGAG 2 cut(s) 251, 611
BssT1I CCWWGG 2 cut(s) 201, 677
Bst1107I GTATAC 1 cut(s) 1470
Bst2BI CACGAG 2 cut(s) 251, 611
Bst2UI CCWGG 2 cut(s) 414, 1790
Bst4CI ACNGT 3 cut(s) 21, 1204, 1265
Bst6I CTCTTC 2 cut(s) 465, 2127
BstACI GRCGYC 1 cut(s) 806
BstAFI CTTAAG 1 cut(s) 1037
BstBI TTCGAA 2 cut(s) 1213, 2021
BstC8I GCNNGC 6 cut(s) 14, 340, 454, 692, 1050, 1714
BstDEI CTNAG 4 cut(s) 171, 344, 1658, 1946
BstDSI CCRYGG 1 cut(s) 677
BstF5I GGATG 1 cut(s) 1377
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 2 cut(s) 12, 877
BstMWI GCNNNNNNNGC 1 cut(s) 650
BstNI CCWGG 2 cut(s) 414, 1790
BstNSI RCATGY 6 cut(s) 456, 490, 922, 1052, 1538, 1547
BstSCI CCNGG 3 cut(s) 262, 412, 1788
BstSFI CTRYAG 2 cut(s) 17, 1082
BstV1I GCAGC 4 cut(s) 387, 614, 628, 1039
BstV2I GAAGAC 1 cut(s) 755
BstX2I RGATCY 3 cut(s) 101, 845, 2184
BstYI RGATCY 3 cut(s) 101, 845, 2184
BstZ17I GTATAC 1 cut(s) 1470
Bsu15I ATCGAT 1 cut(s) 2042
Bsu36I CCTNAGG 1 cut(s) 344
BsuI GTATCC 2 cut(s) 1408, 1778
BsuRI GGCC 4 cut(s) 120, 181, 1127, 2028
BsuTUI ATCGAT 1 cut(s) 2042
BtgI CCRYGG 1 cut(s) 677
BtsCI GGATG 1 cut(s) 1377
BtsI GCAGTG 2 cut(s) 1284, 1735
BtsIMutI CAGTG 7 cut(s) 286, 720, 1284, 1353, 1500, 1735, 1883
BveI ACCTGC 2 cut(s) 631, 734
Cac8I GCNNGC 6 cut(s) 14, 340, 454, 692, 1050, 1714
CciI TCATGA 1 cut(s) 1588
CfoI GCGC 2 cut(s) 12, 877
Cfr13I GGNCC 4 cut(s) 353, 554, 620, 761
ClaI ATCGAT 1 cut(s) 2042
CsiI ACCWGGT 1 cut(s) 1788
Csp6I GTAC 6 cut(s) 165, 271, 1511, 1894, 2047, 2169
CviQI GTAC 6 cut(s) 165, 271, 1511, 1894, 2047, 2169
DdeI CTNAG 4 cut(s) 171, 344, 1658, 1946
EaeI YGGCCR 2 cut(s) 118, 179
Eam1104I CTCTTC 2 cut(s) 465, 2127
EarI CTCTTC 2 cut(s) 465, 2127
EciI GGCGGA 1 cut(s) 843
Eco130I CCWWGG 2 cut(s) 201, 677
Eco147I AGGCCT 1 cut(s) 1127
Eco24I GRGCYC 1 cut(s) 444
Eco47I GGWCC 4 cut(s) 353, 554, 620, 761
Eco81I CCTNAGG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 1684
EcoO109I RGGNCCY 1 cut(s) 620
EcoRII CCWGG 2 cut(s) 412, 1788
EcoT14I CCWWGG 2 cut(s) 201, 677
EcoT22I ATGCAT 1 cut(s) 1780
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 2 cut(s) 201, 677
FalI AAGNNNNNCTT 4 cut(s) 198, 230, 2158, 2190
FaqI GGGAC 1 cut(s) 395
FauNDI CATATG 2 cut(s) 379, 961
FbaI TGATCA 2 cut(s) 717, 982
FblI GTMKAC 2 cut(s) 657, 1469
Fnu4HI GCNGC 4 cut(s) 401, 603, 642, 1053
FokI GGATG 1 cut(s) 1364
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 4 cut(s) 401, 603, 642, 1053
FspBI CTAG 3 cut(s) 296, 995, 1172
GlaI GCGC 2 cut(s) 11, 876
GluI GCNGC 4 cut(s) 401, 603, 642, 1053
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 4 cut(s) 120, 181, 1127, 2028
HapII CCGG 2 cut(s) 263, 1679
HhaI GCGC 2 cut(s) 12, 877
Hin1I GRCGYC 1 cut(s) 806
Hin6I GCGC 2 cut(s) 10, 875
HinP1I GCGC 2 cut(s) 10, 875
HincII GTYRAC 2 cut(s) 658, 1115
HindII GTYRAC 2 cut(s) 658, 1115
HindIII AAGCTT 1 cut(s) 1968
HinfI GANTC 7 cut(s) 130, 223, 1343, 1523, 1615, 1682, 2075
HpaII CCGG 2 cut(s) 263, 1679
HphI GGTGA 3 cut(s) 694, 762, 1306
Hpy166II GTNNAC 8 cut(s) 167, 424, 514, 658, 792, 910, 1115, 1470
Hpy188III TCNNGA 8 cut(s) 251, 707, 1121, 1139, 1552, 1589, 1697, 1838
Hpy8I GTNNAC 8 cut(s) 167, 424, 514, 658, 792, 910, 1115, 1470
Hpy99I CGWCG 2 cut(s) 98, 119
HpyAV CCTTC 5 cut(s) 200, 257, 1537, 1859, 2043
HpyCH4III ACNGT 3 cut(s) 21, 1204, 1265
HpyCH4IV ACGT 5 cut(s) 630, 806, 870, 1437, 2052
HpyF10VI GCNNNNNNNGC 1 cut(s) 650
HpyF3I CTNAG 4 cut(s) 171, 344, 1658, 1946
HpySE526I ACGT 5 cut(s) 630, 806, 870, 1437, 2052
Hsp92I GRCGYC 1 cut(s) 806
HspAI GCGC 2 cut(s) 10, 875
Ksp22I TGATCA 2 cut(s) 717, 982
LmnI GCTCC 2 cut(s) 709, 994
Lsp1109I GCAGC 4 cut(s) 387, 614, 628, 1039
LweI GCATC 3 cut(s) 691, 1224, 1900
MabI ACCWGGT 1 cut(s) 1788
MaeI CTAG 3 cut(s) 296, 995, 1172
MaeII ACGT 5 cut(s) 630, 806, 870, 1437, 2052
MaeIII GTNAC 5 cut(s) 85, 1438, 1537, 1640, 1797
MfeI CAATTG 1 cut(s) 1043
MflI RGATCY 3 cut(s) 101, 845, 2184
MhlI GDGCHC 2 cut(s) 444, 1431
MlsI TGGCCA 1 cut(s) 181
MluNI TGGCCA 1 cut(s) 181
MlyI GAGTC 2 cut(s) 1532, 1676
MmeI TCCRAC 1 cut(s) 1651
Mox20I TGGCCA 1 cut(s) 181
Mph1103I ATGCAT 1 cut(s) 1780
MscI TGGCCA 1 cut(s) 181
MseI TTAA 8 cut(s) 447, 1013, 1038, 1628, 1875, 1940, 1977, 2069
MslI CAYNNNNRTG 1 cut(s) 1436
Msp20I TGGCCA 1 cut(s) 181
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 2 cut(s) 263, 1679
MspR9I CCNGG 3 cut(s) 264, 414, 1790
MunI CAATTG 1 cut(s) 1043
Mva1269I GAATGC 3 cut(s) 605, 741, 1251
MvaI CCWGG 2 cut(s) 414, 1790
MwoI GCNNNNNNNGC 1 cut(s) 650
NciI CCSGG 1 cut(s) 264
NcoI CCATGG 1 cut(s) 677
NdeI CATATG 2 cut(s) 379, 961
NlaIV GGNNCC 1 cut(s) 245
NmeAIII GCCGAG 1 cut(s) 868
NmuCI GTSAC 2 cut(s) 85, 1438
NsiI ATGCAT 1 cut(s) 1780
NspI RCATGY 6 cut(s) 456, 490, 922, 1052, 1538, 1547
NspV TTCGAA 2 cut(s) 1213, 2021
PaeI GCATGC 2 cut(s) 456, 1052
PagI TCATGA 1 cut(s) 1588
PceI AGGCCT 1 cut(s) 1127
PciI ACATGT 2 cut(s) 918, 1534
PctI GAATGC 3 cut(s) 605, 741, 1251
PfeI GAWTC 5 cut(s) 130, 223, 1343, 1615, 2075
PkrI GCNGC 4 cut(s) 402, 604, 643, 1054
PleI GAGTC 2 cut(s) 1531, 1676
PpsI GAGTC 2 cut(s) 1531, 1676
PpuMI RGGWCCY 1 cut(s) 620
PscI ACATGT 2 cut(s) 918, 1534
PshBI ATTAAT 1 cut(s) 1977
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 2 cut(s) 412, 1788
PspGI CCWGG 2 cut(s) 412, 1788
PspN4I GGNNCC 1 cut(s) 245
PspPI GGNCC 4 cut(s) 353, 554, 620, 761
PspPPI RGGWCCY 1 cut(s) 620
PstI CTGCAG 1 cut(s) 1086
PsuI RGATCY 3 cut(s) 101, 845, 2184
RsaI GTAC 6 cut(s) 166, 272, 1512, 1895, 2048, 2170
RsaNI GTAC 6 cut(s) 165, 271, 1511, 1894, 2047, 2169
RseI CAYNNNNRTG 1 cut(s) 1436
SalI GTCGAC 1 cut(s) 656
SaqAI TTAA 8 cut(s) 447, 1013, 1038, 1628, 1875, 1940, 1977, 2069
SatI GCNGC 4 cut(s) 401, 603, 642, 1053
Sau96I GGNCC 4 cut(s) 353, 554, 620, 761
ScaI AGTACT 2 cut(s) 1512, 2170
SchI GAGTC 2 cut(s) 1532, 1676
ScrFI CCNGG 3 cut(s) 264, 414, 1790
SduI GDGCHC 2 cut(s) 444, 1431
SexAI ACCWGGT 1 cut(s) 1788
SfaNI GCATC 3 cut(s) 691, 1224, 1900
SfcI CTRYAG 2 cut(s) 17, 1082
SfuI TTCGAA 2 cut(s) 1213, 2021
SinI GGWCC 4 cut(s) 353, 554, 620, 761
SmiMI CAYNNNNRTG 1 cut(s) 1436
SmlI CTYRAG 2 cut(s) 1037, 1424
SmoI CTYRAG 2 cut(s) 1037, 1424
SphI GCATGC 2 cut(s) 456, 1052
SseBI AGGCCT 1 cut(s) 1127
SsiI CCGC 3 cut(s) 427, 828, 992
SspI AATATT 3 cut(s) 859, 1648, 1861
SspMI CTAG 3 cut(s) 296, 995, 1172
StuI AGGCCT 1 cut(s) 1127
StyD4I CCNGG 3 cut(s) 262, 412, 1788
StyI CCWWGG 2 cut(s) 201, 677
TaaI ACNGT 3 cut(s) 21, 1204, 1265
TaiI ACGT 5 cut(s) 633, 809, 873, 1440, 2055
TatI WGTACW 3 cut(s) 164, 1510, 2168
TfiI GAWTC 5 cut(s) 130, 223, 1343, 1615, 2075
Tru1I TTAA 8 cut(s) 447, 1013, 1038, 1628, 1875, 1940, 1977, 2069
Tru9I TTAA 8 cut(s) 447, 1013, 1038, 1628, 1875, 1940, 1977, 2069
TscAI CASTG 7 cut(s) 286, 720, 1291, 1353, 1507, 1742, 1890
TseFI GTSAC 2 cut(s) 85, 1438
TseI GCWGC 4 cut(s) 400, 602, 641, 1052
Tsp45I GTSAC 2 cut(s) 85, 1438
TspDTI ATGAA 9 cut(s) 295, 349, 536, 597, 1014, 1200, 1448, 1594, 1998
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 7 cut(s) 286, 720, 1291, 1353, 1507, 1742, 1890
Vha464I CTTAAG 1 cut(s) 1037
VpaK11BI GGWCC 4 cut(s) 353, 554, 620, 761
VspI ATTAAT 1 cut(s) 1977
XapI RAATTY 4 cut(s) 493, 1384, 1706, 1731
XceI RCATGY 6 cut(s) 456, 490, 922, 1052, 1538, 1547
XmiI GTMKAC 2 cut(s) 657, 1469
XspI CTAG 3 cut(s) 296, 995, 1172
ZraI GACGTC 1 cut(s) 807
ZrmI AGTACT 2 cut(s) 1512, 2170
Zsp2I ATGCAT 1 cut(s) 1780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.