Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010668

Physical Location & Seq

Genomic Coordinates Reverse (-)

10676120 .. 10679495

Gene Structure 3376 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1144600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1917 bp

ATGGCGAGAGGACCTCCCGCTTTTGAGCTATGGATCTCTGTCTGTCTTTTGCTCTTCTTTCATGCACGCTGCTTCTACCTCCCGGGTGTCGCCCCTCAAGATTTCCAATACGGAAATCCCTTGAATGTGAAAGTGAACAAATTGACCTCTACAAAAACTCAACTTCCGTACTCATACTATTCCCTCCCATACTGTACTCCGGAGCATATAGTAGACAGTGCAGAGAATCTTGGGGAAGTTCTCCGTGGTGATCGCATTGAAAACTCTCCTTATGAGTTCAAAATGAGAGAGCCACAAATGTGCAATGCTGTATGCCGTGTAGTTCTTAATGCAAAAACTGCAAAGGAATTCAAGGAAAAGATAGATGATGAGTATCGGGTGAACATGATTCTGGATAATCTCCCTCTGGTTGTTCCTATACCAAGGCCTGATCAGGAAAATGCCTTGGTTTATCAACATGGGTTTCATGTTGGTCTTAGAGGACAATATGCTGGGAACAAGGATGAAAAGTATTTTATCAACAATCACTTAACATTTACAGTCAAATATCACAAGGATCAAATGACAGAATCTGCCAGGATTGTCGGATTTGAGGTGAAGCCATTCAGTGTTAAGCATGAATATGAAGGCGAGTGGAGTAAGGAGAAACGTTTAACAACCTGTGATCCCCATGCAAAACGAACAGTTACCAGCTCTGAATCTCCTCAGGAGGTTGAAGATAAAAAGGAAATTATATTTACATATGATGTTGAGTTCCAGGAGAGTGATGTGAAGTGGGCATCTCGGTGGGACACATATCTTCTGGTGGCGGATGATCAAATCCACTGGTTCTCAATTGTTAATTCTTTGATGATTGTTCTTTTCCTTTCGGGCATGGTTGCTATGATAATGTTGCGGACACTTTACCGGGATATCTCCAAGTACAACCAACTAGAGACCCAAGAAGAAGCCCAAGAAGAGACAGGATGGAAACTGGTTCATGGTGATGTATTCAGACCTCCAGTAAACTCAGACCTACTGTGTGTGTATGTTGGGACGGGTGTGCAGTTCTTTGGGATGATTCTCGTCACCATGCTCTTCGCAGTCCTTGGATTCCTCTCCCCTTCAAACCGAGGTGGATTGATGACTGCCATGCTCCTCCTCTGGGTCTTTATGGGCCTCTTTGCTGGATACTCTGCAGCCCGTCTGTACAAAATGTTTAAGGGAACAGAATGGAAGAAAATCAGTCTCAAAACAGCTTTCATGTTTCCTGCAACTCTCTTTGCCATTTTCTTCGTCTTGAATGCTCTTATCTGGGGCGAGAAATCCTCTGGGGCAGTTCCATTTGGAACCATGTTTGCTCTGGTATTCTTATGGTTTGGCATTTCAGTTCCACTTATCTACGTTGGTGCTTATGTGGGTTTTAGGAAGCCATCGATTGAGGATCCTGTGAAAACCAACAAGATCCCTAGGCAAGTCCCTGAGCAGGCGTGGTACATGCATCCAGCCTTCTCTATCCTAATTGGAGGCATACTCCCATTCGGGGCCGTCTTTATTGAGCTCTTCTTCATCCTTACATCTATATGGTTGCATCAATTCTATTACATATTTGGATTCCTTTTCATCGTCTTCATTATCCTCATCATCACTTGTGCCGAGATCACAATTGTGCTATGCTACTTCCAGTTGTGCAGTGAAGACTACCTTTGGTGGTGGAGATCGTATTTGACCTCAGGCTCCTCAGCGCTCTACCTTTTCCTGTACGCAGCCTTCTACTTCTTCACTAAGCTTGACATTAAGAAGCCGGTTTCTGGAGCCTTGTATTTCGGGTATATGCTGATTGCTTCCTATTCTTTCTTCGTGCTGACTGGTACAATCGGTTTCTATGCATGCTTCTGGTTTACTAGGCTCATCTATTCGTCAGTGAAGTTCGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0008150 GO:0012505 GO:0016020 GO:0016021 GO:0031224 GO:0031410 GO:0031982 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033157 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048518 GO:0050789 GO:0051049 GO:0051050 GO:0051222 GO:0051223 GO:0060341 GO:0065007 GO:0070201 GO:0070861 GO:0070863 GO:0090087 GO:0090316 GO:0097708 GO:1903827 GO:1903829 GO:1904951 GO:2000008 GO:2000010

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

639

Amino Acids

73.69

Weight (kDa)

6.65

Isoelectric Point (pI)

39.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	56 - 593	1.9e-222	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010668)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24170 AT2G24170
fragaria_vesca	FvH4_1g00890 FvH4_1g00890 FvH4_1g00890
malus_domestica	MD02G1007300.v1.1 MD15G1144600.v1.1
prunus_persica	Prupe.7G263100_v2.0.a1
pyrus_communis	pycom02g00630 pycom15g13620
rosa_chinensis	RchiOBHm_Chr2g0085601
rosa_laevigata	RLG00000015704
rosa_multiflora	Rmu_sc0016087.1_g000003
rosa_roxburghii	Rroxscaffold_2G00155230
rosa_rugosa	Rorug01G0461500
rosa_samantha	Rh2AG010800 Rh2BG011200 Rh2CG012100 Rh2DG013200
rosa_wichuraiana	Rw2G000960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	213
AccIII	TCCGGA	1 cut(s)	199
AciI	CCGC	3 cut(s)	18, 809, 895
AclI	AACGTT	1 cut(s)	649
AclWI	GGATC	6 cut(s)	41, 564, 659, 1418, 1431, 1438
AcsI	RAATTY	1 cut(s)	347
AfaI	GTAC	7 cut(s)	170, 196, 923, 1190, 1475, 1742, 1852
AfeI	AGCGCT	1 cut(s)	1725
AfiI	CCNNNNNNNGG	4 cut(s)	1144, 1465, 1522, 1790
AgsI	TTSAA	7 cut(s)	124, 260, 280, 352, 716, 1107, 1282
AjnI	CCWGG	2 cut(s)	575, 756
AleI	CACNNNNGTG	2 cut(s)	298, 1646
AluBI	AGCT	5 cut(s)	28, 693, 1238, 1540, 1768
AluI	AGCT	5 cut(s)	28, 693, 1238, 1540, 1768
Alw21I	GWGCWC	1 cut(s)	1542
Alw26I	GTCTC	3 cut(s)	929, 953, 1232
AlwI	GGATC	6 cut(s)	41, 564, 659, 1418, 1431, 1438
AlwNI	CAGNNNCTG	1 cut(s)	572
Ama87I	CYCGRG	1 cut(s)	82
Aor13HI	TCCGGA	1 cut(s)	199
Aor51HI	AGCGCT	1 cut(s)	1725
AoxI	GGCC	3 cut(s)	425, 1156, 1524
ApeKI	GCWGC	3 cut(s)	69, 1178, 1745
ApoI	RAATTY	1 cut(s)	347
Asp700I	GAANNNNTTC	1 cut(s)	602
AspA2I	CCTAGG	1 cut(s)	1448
AspLEI	GCGC	1 cut(s)	1726
AspS9I	GGNCC	3 cut(s)	11, 1156, 1524
AsuC2I	CCSGG	3 cut(s)	83, 84, 908
AsuHPI	GGTGA	5 cut(s)	260, 391, 607, 995, 1060
AvaI	CYCGRG	1 cut(s)	82
AvaII	GGWCC	1 cut(s)	11
AvrII	CCTAGG	1 cut(s)	1448
AxyI	CCTNAGG	2 cut(s)	705, 1711
BamHI	GGATCC	1 cut(s)	1423
BanII	GRGCYC	1 cut(s)	1542
BarI	GAAGNNNNNNTAC	2 cut(s)	1733, 1765
BbsI	GAAGAC	2 cut(s)	1600, 1683
Bbv12I	GWGCWC	1 cut(s)	1542
BbvCI	CCTCAGC	1 cut(s)	1720
BbvI	GCAGC	3 cut(s)	56, 1190, 1757
BccI	CCATC	2 cut(s)	960, 1420
BceAI	ACGGC	2 cut(s)	300, 1511
BciT130I	CCWGG	2 cut(s)	577, 758
BciVI	GTATCC	1 cut(s)	1163
BclI	TGATCA	2 cut(s)	430, 814
BcnI	CCSGG	3 cut(s)	83, 84, 908
BcoDI	GTCTC	3 cut(s)	929, 953, 1232
BfaI	CTAG	3 cut(s)	932, 1449, 1884
BfmI	CTRYAG	1 cut(s)	1176
BfoI	RGCGCY	1 cut(s)	1727
BfuI	GTATCC	1 cut(s)	1163
BisI	GCNGC	3 cut(s)	70, 1179, 1746
BlnI	CCTAGG	1 cut(s)	1448
BlsI	GCNGC	3 cut(s)	71, 1180, 1747
Bme1390I	CCNGG	5 cut(s)	83, 84, 577, 758, 908
Bme18I	GGWCC	1 cut(s)	11
BmeT110I	CYCGRG	1 cut(s)	82
BmgT120I	GGNCC	3 cut(s)	11, 1156, 1524
BmiI	GGNNCC	5 cut(s)	1330, 1425, 1525, 1717, 1795
BmrFI	CCNGG	5 cut(s)	83, 84, 577, 758, 908
BmsI	GCATC	3 cut(s)	788, 1489, 1579
BpiI	GAAGAC	2 cut(s)	1600, 1683
BplI	GAGNNNNNCTC	5 cut(s)	30, 1292, 1324, 1497, 1529
BpmI	CTGGAG	2 cut(s)	984, 1812
Bpu10I	CCTNAGC	2 cut(s)	1461, 1720
BpuEI	CTTGAG	1 cut(s)	81
BpuMI	CCSGG	3 cut(s)	83, 84, 908
Bsa29I	ATCGAT	1 cut(s)	1415
BsaBI	GATNNNNATC	1 cut(s)	372
BsaI	GGTCTC	1 cut(s)	929
BsaJI	CCNNGG	7 cut(s)	82, 244, 422, 444, 1087, 1111, 1448
BsaWI	WCCGGW	1 cut(s)	199
Bsc4I	CCNNNNNNNGG	4 cut(s)	1144, 1465, 1522, 1790
Bse118I	RCCGGY	1 cut(s)	1783
Bse1I	ACTGG	5 cut(s)	830, 978, 1001, 1663, 1852
Bse21I	CCTNAGG	2 cut(s)	705, 1711
Bse3DI	GCAATG	1 cut(s)	310
Bse8I	GATNNNNATC	1 cut(s)	372
BseAI	TCCGGA	1 cut(s)	199
BseBI	CCWGG	2 cut(s)	577, 758
BseCI	ATCGAT	1 cut(s)	1415
BseDI	CCNNGG	7 cut(s)	82, 244, 422, 444, 1087, 1111, 1448
BseGI	GGATG	6 cut(s)	508, 817, 971, 1062, 1480, 1548
BseJI	GATNNNNATC	1 cut(s)	372
BseLI	CCNNNNNNNGG	4 cut(s)	1144, 1465, 1522, 1790
BseMI	GCAATG	1 cut(s)	310
BseMII	CTCAG	5 cut(s)	719, 1023, 1452, 1725, 1734
BseNI	ACTGG	5 cut(s)	830, 978, 1001, 1663, 1852
BseRI	GAGGAG	4 cut(s)	693, 1127, 1130, 1708
BseXI	GCAGC	3 cut(s)	56, 1190, 1757
BseYI	CCCAGC	1 cut(s)	491
BsgI	GTGCAG	3 cut(s)	240, 1064, 1690
BshFI	GGCC	3 cut(s)	427, 1158, 1526
BshVI	ATCGAT	1 cut(s)	1415
BsiHKAI	GWGCWC	1 cut(s)	1542
BsiHKCI	CYCGRG	1 cut(s)	82
BsiSI	CCGG	4 cut(s)	83, 200, 907, 1784
BslFI	GGGAC	3 cut(s)	803, 1048, 1442
BslI	CCNNNNNNNGG	4 cut(s)	1144, 1465, 1522, 1790
BsmAI	GTCTC	3 cut(s)	929, 953, 1232
BsmFI	GGGAC	3 cut(s)	803, 1048, 1442
BsmI	GAATGC	1 cut(s)	1288
BsnI	GGCC	3 cut(s)	427, 1158, 1526
Bso31I	GGTCTC	1 cut(s)	929
BsoBI	CYCGRG	1 cut(s)	82
Bsp1286I	GDGCHC	1 cut(s)	1542
Bsp13I	TCCGGA	1 cut(s)	199
Bsp1407I	TGTACA	1 cut(s)	1188
BspACI	CCGC	3 cut(s)	18, 809, 895
BspANI	GGCC	3 cut(s)	427, 1158, 1526
BspCNI	CTCAG	5 cut(s)	718, 1022, 1453, 1724, 1733
BspDI	ATCGAT	1 cut(s)	1415
BspEI	TCCGGA	1 cut(s)	199
BspLI	GGNNCC	5 cut(s)	1330, 1425, 1525, 1717, 1795
BspMAI	CTGCAG	1 cut(s)	1180
BspPI	GGATC	6 cut(s)	41, 564, 659, 1418, 1431, 1438
BspQI	GCTCTTC	3 cut(s)	59, 1082, 1547
BspTNI	GGTCTC	1 cut(s)	929
BsrDI	GCAATG	1 cut(s)	310
BsrFI	RCCGGY	1 cut(s)	1783
BsrGI	TGTACA	1 cut(s)	1188
BsrI	ACTGG	5 cut(s)	830, 978, 1001, 1663, 1852
BssAI	RCCGGY	1 cut(s)	1783
BssECI	CCNNGG	7 cut(s)	82, 244, 422, 444, 1087, 1111, 1448
BssT1I	CCWWGG	4 cut(s)	422, 444, 1087, 1448
Bst2UI	CCWGG	2 cut(s)	577, 758
Bst4CI	ACNGT	5 cut(s)	194, 218, 541, 685, 1020
Bst6I	CTCTTC	4 cut(s)	59, 951, 1082, 1547
BstAPI	GCANNNNNTGC	1 cut(s)	338
BstAUI	TGTACA	1 cut(s)	1188
BstC8I	GCNNGC	3 cut(s)	67, 1467, 1870
BstDEI	CTNAG	7 cut(s)	476, 705, 1009, 1461, 1711, 1720, 1764
BstDSI	CCRYGG	1 cut(s)	244
BstF5I	GGATG	6 cut(s)	508, 817, 971, 1062, 1480, 1548
BstH2I	RGCGCY	1 cut(s)	1727
BstHHI	GCGC	1 cut(s)	1726
BstMAI	GTCTC	3 cut(s)	929, 953, 1232
BstMWI	GCNNNNNNNGC	1 cut(s)	338
BstNI	CCWGG	2 cut(s)	577, 758
BstNSI	RCATGY	2 cut(s)	1480, 1872
BstSCI	CCNGG	5 cut(s)	81, 82, 575, 756, 906
BstSFI	CTRYAG	1 cut(s)	1176
BstV1I	GCAGC	3 cut(s)	56, 1190, 1757
BstV2I	GAAGAC	2 cut(s)	1600, 1683
BstX2I	RGATCY	3 cut(s)	33, 1423, 1443
BstYI	RGATCY	3 cut(s)	33, 1423, 1443
Bsu15I	ATCGAT	1 cut(s)	1415
Bsu36I	CCTNAGG	2 cut(s)	705, 1711
BsuI	GTATCC	1 cut(s)	1163
BsuRI	GGCC	3 cut(s)	427, 1158, 1526
BsuTUI	ATCGAT	1 cut(s)	1415
BtgI	CCRYGG	1 cut(s)	244
BtsCI	GGATG	6 cut(s)	508, 817, 971, 1062, 1480, 1548
BtsI	GCAGTG	1 cut(s)	1678
BtsIMutI	CAGTG	5 cut(s)	223, 613, 823, 1678, 1908
Cac8I	GCNNGC	3 cut(s)	67, 1467, 1870
CaiI	CAGNNNCTG	1 cut(s)	572
CfoI	GCGC	1 cut(s)	1726
Cfr10I	RCCGGY	1 cut(s)	1783
Cfr13I	GGNCC	3 cut(s)	11, 1156, 1524
Cfr9I	CCCGGG	1 cut(s)	82
ClaI	ATCGAT	1 cut(s)	1415
Csp6I	GTAC	7 cut(s)	169, 195, 922, 1189, 1474, 1741, 1851
CviQI	GTAC	7 cut(s)	169, 195, 922, 1189, 1474, 1741, 1851
DdeI	CTNAG	7 cut(s)	476, 705, 1009, 1461, 1711, 1720, 1764
Eam1104I	CTCTTC	4 cut(s)	59, 951, 1082, 1547
EarI	CTCTTC	4 cut(s)	59, 951, 1082, 1547
EciI	GGCGGA	1 cut(s)	824
Ecl136II	GAGCTC	1 cut(s)	1540
Eco130I	CCWWGG	4 cut(s)	422, 444, 1087, 1448
Eco147I	AGGCCT	1 cut(s)	427
Eco24I	GRGCYC	1 cut(s)	1542
Eco31I	GGTCTC	1 cut(s)	929
Eco32I	GATATC	1 cut(s)	913
Eco47I	GGWCC	1 cut(s)	11
Eco47III	AGCGCT	1 cut(s)	1725
Eco53kI	GAGCTC	1 cut(s)	1540
Eco81I	CCTNAGG	2 cut(s)	705, 1711
Eco88I	CYCGRG	1 cut(s)	82
EcoICRI	GAGCTC	1 cut(s)	1540
EcoO109I	RGGNCCY	1 cut(s)	11
EcoRI	GAATTC	1 cut(s)	347
EcoRII	CCWGG	2 cut(s)	575, 756
EcoRV	GATATC	1 cut(s)	913
EcoT14I	CCWWGG	4 cut(s)	422, 444, 1087, 1448
EcoT22I	ATGCAT	2 cut(s)	1482, 1870
EcoT38I	GRGCYC	1 cut(s)	1542
ErhI	CCWWGG	4 cut(s)	422, 444, 1087, 1448
FalI	AAGNNNNNCTT	2 cut(s)	1668, 1700
FaqI	GGGAC	3 cut(s)	803, 1048, 1442
FauI	CCCGC	1 cut(s)	25
FauNDI	CATATG	1 cut(s)	742
FbaI	TGATCA	2 cut(s)	430, 814
FblI	GTMKAC	1 cut(s)	213
Fnu4HI	GCNGC	3 cut(s)	70, 1179, 1746
FokI	GGATG	6 cut(s)	515, 824, 978, 1069, 1467, 1535
FriOI	GRGCYC	1 cut(s)	1542
Fsp4HI	GCNGC	3 cut(s)	70, 1179, 1746
FspBI	CTAG	3 cut(s)	932, 1449, 1884
GlaI	GCGC	1 cut(s)	1725
GluI	GCNGC	3 cut(s)	70, 1179, 1746
GsaI	CCCAGC	1 cut(s)	495
GsuI	CTGGAG	2 cut(s)	984, 1812
HaeII	RGCGCY	1 cut(s)	1727
HaeIII	GGCC	3 cut(s)	427, 1158, 1526
HapII	CCGG	4 cut(s)	83, 200, 907, 1784
HhaI	GCGC	1 cut(s)	1726
Hin6I	GCGC	1 cut(s)	1724
HinP1I	GCGC	1 cut(s)	1724
HindIII	AAGCTT	1 cut(s)	1766
HinfI	GANTC	7 cut(s)	226, 388, 569, 698, 1060, 1092, 1593
HpaII	CCGG	4 cut(s)	83, 200, 907, 1784
HphI	GGTGA	5 cut(s)	260, 391, 607, 995, 1060
Hpy166II	GTNNAC	5 cut(s)	136, 214, 382, 1006, 1881
Hpy188I	TCNGA	4 cut(s)	587, 697, 995, 1012
Hpy188III	TCNNGA	7 cut(s)	98, 200, 392, 434, 707, 1279, 1791
Hpy8I	GTNNAC	5 cut(s)	136, 214, 382, 1006, 1881
HpyAV	CCTTC	4 cut(s)	620, 1113, 1498, 1759
HpyCH4III	ACNGT	5 cut(s)	194, 218, 541, 685, 1020
HpyCH4IV	ACGT	2 cut(s)	649, 1383
HpyF10VI	GCNNNNNNNGC	1 cut(s)	338
HpyF3I	CTNAG	7 cut(s)	476, 705, 1009, 1461, 1711, 1720, 1764
HpySE526I	ACGT	2 cut(s)	649, 1383
HspAI	GCGC	1 cut(s)	1724
Kpn2I	TCCGGA	1 cut(s)	199
Ksp22I	TGATCA	2 cut(s)	430, 814
LguI	GCTCTTC	3 cut(s)	59, 1082, 1547
LmnI	GCTCC	4 cut(s)	202, 1140, 1721, 1793
Lsp1109I	GCAGC	3 cut(s)	56, 1190, 1757
LweI	GCATC	3 cut(s)	788, 1489, 1579
MaeI	CTAG	3 cut(s)	932, 1449, 1884
MaeII	ACGT	2 cut(s)	649, 1383
MaeIII	GTNAC	2 cut(s)	685, 1066
MfeI	CAATTG	2 cut(s)	834, 1644
MflI	RGATCY	3 cut(s)	33, 1423, 1443
MhlI	GDGCHC	1 cut(s)	1542
MluCI	AATT	8 cut(s)	140, 347, 729, 834, 841, 1500, 1574, 1644
MmeI	TCCRAC	1 cut(s)	565
Mph1103I	ATGCAT	2 cut(s)	1482, 1870
MroI	TCCGGA	1 cut(s)	199
MroXI	GAANNNNTTC	1 cut(s)	602
MseI	TTAA	7 cut(s)	327, 530, 612, 653, 840, 1200, 1776
MslI	CAYNNNNRTG	6 cut(s)	298, 621, 784, 984, 1561, 1646
MspI	CCGG	4 cut(s)	83, 200, 907, 1784
MspR9I	CCNGG	5 cut(s)	83, 84, 577, 758, 908
MunI	CAATTG	2 cut(s)	834, 1644
Mva1269I	GAATGC	1 cut(s)	1288
MvaI	CCWGG	2 cut(s)	577, 758
MwoI	GCNNNNNNNGC	1 cut(s)	338
NciI	CCSGG	3 cut(s)	83, 84, 908
NdeI	CATATG	1 cut(s)	742
NlaIV	GGNNCC	5 cut(s)	1330, 1425, 1525, 1717, 1795
NmeAIII	GCCGAG	1 cut(s)	1660
NmuCI	GTSAC	1 cut(s)	1066
NsiI	ATGCAT	2 cut(s)	1482, 1870
NspI	RCATGY	2 cut(s)	1480, 1872
OliI	CACNNNNGTG	2 cut(s)	298, 1646
PaeI	GCATGC	1 cut(s)	1872
PceI	AGGCCT	1 cut(s)	427
PciSI	GCTCTTC	3 cut(s)	59, 1082, 1547
PctI	GAATGC	1 cut(s)	1288
PdmI	GAANNNNTTC	1 cut(s)	602
PfeI	GAWTC	7 cut(s)	226, 388, 569, 698, 1060, 1092, 1593
PfoI	TCCNGGA	1 cut(s)	756
PkrI	GCNGC	3 cut(s)	71, 1180, 1747
PpuMI	RGGWCCY	1 cut(s)	11
Psp124BI	GAGCTC	1 cut(s)	1542
Psp1406I	AACGTT	1 cut(s)	649
Psp5II	RGGWCCY	1 cut(s)	11
Psp6I	CCWGG	2 cut(s)	575, 756
PspFI	CCCAGC	1 cut(s)	491
PspGI	CCWGG	2 cut(s)	575, 756
PspN4I	GGNNCC	5 cut(s)	1330, 1425, 1525, 1717, 1795
PspPI	GGNCC	3 cut(s)	11, 1156, 1524
PspPPI	RGGWCCY	1 cut(s)	11
PstI	CTGCAG	1 cut(s)	1180
PstNI	CAGNNNCTG	1 cut(s)	572
PsuI	RGATCY	3 cut(s)	33, 1423, 1443
RsaI	GTAC	7 cut(s)	170, 196, 923, 1190, 1475, 1742, 1852
RsaNI	GTAC	7 cut(s)	169, 195, 922, 1189, 1474, 1741, 1851
RseI	CAYNNNNRTG	6 cut(s)	298, 621, 784, 984, 1561, 1646
SacI	GAGCTC	1 cut(s)	1542
SapI	GCTCTTC	3 cut(s)	59, 1082, 1547
SaqAI	TTAA	7 cut(s)	327, 530, 612, 653, 840, 1200, 1776
SatI	GCNGC	3 cut(s)	70, 1179, 1746
Sau96I	GGNCC	3 cut(s)	11, 1156, 1524
ScrFI	CCNGG	5 cut(s)	83, 84, 577, 758, 908
SduI	GDGCHC	1 cut(s)	1542
SfaNI	GCATC	3 cut(s)	788, 1489, 1579
SfcI	CTRYAG	1 cut(s)	1176
SinI	GGWCC	1 cut(s)	11
SmaI	CCCGGG	1 cut(s)	84
SmiMI	CAYNNNNRTG	6 cut(s)	298, 621, 784, 984, 1561, 1646
SmlI	CTYRAG	1 cut(s)	96
SmoI	CTYRAG	1 cut(s)	96
SphI	GCATGC	1 cut(s)	1872
Sse9I	AATT	8 cut(s)	140, 347, 729, 834, 841, 1500, 1574, 1644
SseBI	AGGCCT	1 cut(s)	427
SsiI	CCGC	3 cut(s)	18, 809, 895
SspMI	CTAG	3 cut(s)	932, 1449, 1884
SstI	GAGCTC	1 cut(s)	1542
StuI	AGGCCT	1 cut(s)	427
StyD4I	CCNGG	5 cut(s)	81, 82, 575, 756, 906
StyI	CCWWGG	4 cut(s)	422, 444, 1087, 1448
TaaI	ACNGT	5 cut(s)	194, 218, 541, 685, 1020
TaiI	ACGT	2 cut(s)	652, 1386
TaqI	TCGA	2 cut(s)	1415, 1911
TasI	AATT	8 cut(s)	140, 347, 729, 834, 841, 1500, 1574, 1644
TatI	WGTACW	3 cut(s)	194, 921, 1188
TfiI	GAWTC	7 cut(s)	226, 388, 569, 698, 1060, 1092, 1593
Tru1I	TTAA	7 cut(s)	327, 530, 612, 653, 840, 1200, 1776
Tru9I	TTAA	7 cut(s)	327, 530, 612, 653, 840, 1200, 1776
TscAI	CASTG	5 cut(s)	223, 613, 830, 1678, 1908
TseFI	GTSAC	1 cut(s)	1066
TseI	GCWGC	3 cut(s)	69, 1178, 1745
Tsp45I	GTSAC	1 cut(s)	1066
TspGWI	ACGGA	3 cut(s)	126, 156, 233
TspMI	CCCGGG	1 cut(s)	82
TspRI	CASTG	5 cut(s)	223, 613, 830, 1678, 1908
VpaK11BI	GGWCC	1 cut(s)	11
XapI	RAATTY	1 cut(s)	347
XceI	RCATGY	2 cut(s)	1480, 1872
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1339
XmaI	CCCGGG	1 cut(s)	82
XmaJI	CCTAGG	1 cut(s)	1448
XmiI	GTMKAC	1 cut(s)	213
XmnI	GAANNNNTTC	1 cut(s)	602
XspI	CTAG	3 cut(s)	932, 1449, 1884
Zsp2I	ATGCAT	2 cut(s)	1482, 1870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.