MD15G1249300.v1.1

Transmembrane and coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008389
Physical Location & Seq
Forward (+)
20803300 .. 20807754
4455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1249300.v1.1.491

Sequence Viewer

Length: 2271 bp
ATGTATATCAAATGTATCGAAAACAAGTCTTCACGTTGCCGCGTGGACAAGCAATGTAGAAGTAGTCAGGACAAATTTCAGGATTTCAAAAAAATTTCAGTTCATTCGAGGCTTCAGGTGGCATCACTCATGGCCCCGCCGTCGTATCTGACGCCGACGCAGCGGTACGCCGCCGGAGCTCTGTTCGCGTTCGCTCTTCATCAGGCTCAGATCCACCAGACGCGCCCTCTGCGATTGTCATCACAAGAAGAAGAGTCGACGGAGGAGCGGACCAGCAGCGTCAGCAGCGCCGACTCCGTCTCCGACGACCCGGATCTCTGGGTCCACGAAAACTCGGGTCTCCTCCCACCCGTTTTCAGGTTTCTGGAGATTGATTCTGCAGCATGGTCTGGACTTCAGGAGAGTGCTGCTACTTCTCCTGCTAGCCATCATGTTGGAGCTTTCTTGAGGTTGCTTGCTGAAGAAGGTGGTCGTGATGGTAAGGATGCTTCAGAAAATTCAGATCAAGAGCTTGCGTTGTCAAAAGGTATTGACGCTATGGCGTCTAGCATGGAAAAGGATCACGAGTCTTCCCAGTCGAAGATGGAAAAGCATCGTGAATATGAAAATGAATGTCGCGAGAAGTTCTCCACAACTGAGGTGAAAGAGAACATTGAGGCGGCGGATGGGAAGTTGGGAACTTCACAGGAGAAAGGGTCCACAGCTGTGGTGAAGCAAAAAAGGGAAGATGTGGATGAGCATTTGGAGAAGCTGATGGAGAAATGCTCCACAGCTAATGTGAAACAAAAAATTGAAGAAGTGGACTCGCAATTGGAAAATCTGCACGGGACTGGTGGTAATCTGGTTAGGGTTAAAGACGCAGTTGTGTCTACCAGTGACATTCGTGACAAAACTATTGGAGAGTTGACAATGCTCAGTTATGAGAGGAAAGTTACAGTTCTCTATGAACTTCTTACAGCTTGTCTGGCTGATAAACGTGAAGAGAAGAAGAAATGTACCCGTCGGAGAAAGGGTTACGATGCTCGGCATCGTGTAGCTCTGCGTTTGCTAGCAACTTGGCTTGACGTCAAATGGATGAAAATGGAAGCCATTGAGACAATGGTTGCTTGCTCTGCAATGGCATTAGTAAAACAAGAAGAAGAAAAAGAAGGAACCCAATCACCAAAAGACAAATGGGCTAAATGGAAGCGTGGTGGTATCATTGGTGCTGCTGCAATAACTGGAGGAGCGTTGTTGACAATAACTGGTGGACTAGCAGCCCCCGCAATTGCTGCAGGACTTGGTGCTCTAGCTCCAACATTGGGCACTATCATCCCAGTCATTGGAGCAAGTGGGTTTGCCGCAGCTGCAACTGCTGCAGGATCTGTTGCTGGTTCTGTTGTTGTTGCTGCATCATTTGGAGCTGCTGGAGCTGGACTCACAGGGAGCAAAATGGCTAGAAGAACTGGAGGTGTCGATCAATTTGAGTTCAAAGCTATTGGAGAAAATCATAACCAGGGCAGGCTAGCAGTCGAGATCTTGGTTTCAGGATTTGTGTTTGATGAGGAAGACTTTATGAAGCCTTGGGAGGGACATGATGACAATTTGGAAAGGTATGCAGTGCTCTGGGAGTCTAAGAATTTAATTGCAGTGAGCACTGCAATTCAGGATTGGCTTACCTCAACACTTGCAATGGGTTTGATGAAGCAAGGTGCAATGATGACTGTGTTAGGCACTCTTGTAACTGCATTTGCTTGGCCAGCAACATTACTTGCAGCTACTGCTTTTATAGACAGCAAATGGGCAATTGCCGTGGACAGGTCAGACAAAGCAGGAAAGCTTCTTGCTGAGGTGTTACTAAAGGGAGTTCATGGGAACAGGCCTGTAACACTTGTGGGTTTCTCACTTGGAGCACGAGTAATTTTCAAGTGTCTCCAGTGTTTGGCAGAAACTGAACATCATGCTGAACTTGTAGAACGAGTTGTTCTGCTTGGAGCACCCATCTCGATTGCAGATGAGAAATGGGAAGCTGCTAGAAAGATGGTGGCTGGAAGATTTGTGAATGCGTACTCCACAAATGATTGGATGCTTGGGGTTGCTTTCCGTGCTAGTCTACTTTCTCGAGGATTAGCTGGAATTCAACCAATCAATGTTCAAGGGATTGAGAATGTGGATGTAACGGACATAATTGATTCGCACTCTTCCTATCTTTGGTCTACAAAGCAGATCCTGGAGCTGGTTGAACTGGAAGGCTATTATCCCGTTCACAGGAGCATGATTTGCATAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF726

Protein Analysis

757

Amino Acids

82.38

Weight (kDa)

6.05

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF726 PF05277 394 - 742 4.6e-98 Protein of unknown function (DUF726)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1068
AccB7I CCANNNNNTGG 2 cut(s) 1322, 1921
AccBSI CCGCTC 1 cut(s) 268
AccI GTMKAC 4 cut(s) 257, 869, 2092, 2195
AccII CGCG 4 cut(s) 42, 188, 223, 618
AciI CCGC 9 cut(s) 40, 137, 163, 171, 268, 659, 662, 1263, 1341
AclWI GGATC 5 cut(s) 205, 321, 567, 1369, 2200
AcoI YGGCCR 1 cut(s) 1736
AcsI RAATTY 5 cut(s) 74, 93, 496, 1618, 2115
AcuI CTGAAG 4 cut(s) 98, 380, 474, 480
AcyI GRCGYC 3 cut(s) 152, 542, 1065
AfaI GTAC 3 cut(s) 167, 997, 2048
AfiI CCNNNNNNNGG 9 cut(s) 357, 1301, 1322, 1568, 1797, 1921, 2190, 2215, 2247
AgsI TTSAA 7 cut(s) 88, 794, 1471, 1906, 2120, 2135, 2222
AjnI CCWGG 2 cut(s) 1494, 2208
AleI CACNNNNGTG 1 cut(s) 704
Alw21I GWGCWC 6 cut(s) 181, 1288, 1605, 1637, 1894, 1978
Alw26I GTCTC 4 cut(s) 304, 344, 1088, 1916
AlwI GGATC 5 cut(s) 205, 321, 567, 1369, 2200
AlwNI CAGNNNCTG 4 cut(s) 1364, 1760, 1931, 2209
Ama87I CYCGRG 2 cut(s) 334, 2100
AoxI GGCC 3 cut(s) 132, 1736, 1859
ApoI RAATTY 5 cut(s) 74, 93, 496, 1618, 2115
AspLEI GCGC 2 cut(s) 225, 290
AspS9I GGNCC 4 cut(s) 133, 270, 322, 696
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 652, 721, 1152
AsuNHI GCTAGC 3 cut(s) 422, 1048, 1504
AvaI CYCGRG 2 cut(s) 334, 2100
AvaII GGWCC 3 cut(s) 270, 322, 696
BaeGI GKGCMC 1 cut(s) 1307
BalI TGGCCA 1 cut(s) 1738
BanII GRGCYC 1 cut(s) 181
BarI GAAGNNNNNNTAC 2 cut(s) 472, 504
BauI CACGAG 2 cut(s) 563, 1893
BbsI GAAGAC 3 cut(s) 21, 561, 1554
Bbv12I GWGCWC 6 cut(s) 181, 1288, 1605, 1637, 1894, 1978
BbvCI CCTCAGC 1 cut(s) 1827
BccI CCATC 7 cut(s) 435, 470, 577, 659, 748, 1988, 2014
BceAI ACGGC 2 cut(s) 124, 1775
BcgI CGANNNNNNTGC 2 cut(s) 1965, 1999
BciT130I CCWGG 2 cut(s) 1496, 2210
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 4 cut(s) 304, 344, 1088, 1916
BfaI CTAG 9 cut(s) 423, 546, 1049, 1253, 1289, 1437, 1505, 2013, 2088
BfmI CTRYAG 3 cut(s) 378, 1272, 1356
BfoI RGCGCY 1 cut(s) 291
BglII AGATCT 1 cut(s) 1515
Bme1390I CCNGG 3 cut(s) 311, 1496, 2210
Bme18I GGWCC 3 cut(s) 270, 322, 696
BmeT110I CYCGRG 2 cut(s) 334, 2100
BmgT120I GGNCC 4 cut(s) 133, 270, 322, 696
BmiI GGNNCC 4 cut(s) 135, 323, 697, 1153
BmrFI CCNGG 3 cut(s) 311, 1496, 2210
BmrI ACTGGG 2 cut(s) 568, 1310
BmsI GCATC 7 cut(s) 131, 475, 601, 1009, 1036, 1400, 2055
BmtI GCTAGC 3 cut(s) 426, 1052, 1508
BmuI ACTGGG 2 cut(s) 568, 1310
BpiI GAAGAC 3 cut(s) 21, 561, 1554
BplI GAGNNNNNCTC 6 cut(s) 611, 643, 749, 781, 1401, 1433
BpmI CTGGAG 6 cut(s) 386, 1242, 1428, 1467, 1898, 2231
Bpu10I CCTNAGC 1 cut(s) 1827
BpuEI CTTGAG 1 cut(s) 466
BpuMI CCSGG 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 238
BsaHI GRCGYC 3 cut(s) 152, 542, 1065
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 1495, 1562, 1791
BsaXI ACNNNNNCTCC 8 cut(s) 284, 314, 680, 710, 997, 1027, 1881, 1911
Bsc4I CCNNNNNNNGG 9 cut(s) 357, 1301, 1322, 1568, 1797, 1921, 2190, 2215, 2247
Bse1I ACTGG 9 cut(s) 574, 835, 873, 1225, 1249, 1316, 1450, 1915, 2229
Bse3DI GCAATG 4 cut(s) 59, 1122, 1677, 1701
Bse8I GATNNNNATC 1 cut(s) 238
BseBI CCWGG 2 cut(s) 1496, 2210
BseDI CCNNGG 3 cut(s) 1495, 1562, 1791
BseGI GGATG 7 cut(s) 490, 670, 739, 1080, 1311, 2070, 2158
BseJI GATNNNNATC 1 cut(s) 238
BseLI CCNNNNNNNGG 9 cut(s) 357, 1301, 1322, 1568, 1797, 1921, 2190, 2215, 2247
BseMI GCAATG 4 cut(s) 59, 1122, 1677, 1701
BseMII CTCAG 4 cut(s) 221, 627, 928, 1818
BseNI ACTGG 9 cut(s) 574, 835, 873, 1225, 1249, 1316, 1450, 1915, 2229
BseRI GAGGAG 3 cut(s) 278, 332, 1239
BseSI GKGCMC 1 cut(s) 1307
BsgI GTGCAG 1 cut(s) 806
Bsh1236I CGCG 4 cut(s) 42, 188, 223, 618
BshFI GGCC 3 cut(s) 134, 1738, 1861
BsiHKAI GWGCWC 6 cut(s) 181, 1288, 1605, 1637, 1894, 1978
BsiHKCI CYCGRG 2 cut(s) 334, 2100
BsiSI CCGG 2 cut(s) 174, 311
BslFI GGGAC 2 cut(s) 841, 1584
BslI CCNNNNNNNGG 9 cut(s) 357, 1301, 1322, 1568, 1797, 1921, 2190, 2215, 2247
BsmAI GTCTC 4 cut(s) 304, 344, 1088, 1916
BsmBI CGTCTC 1 cut(s) 304
BsmFI GGGAC 2 cut(s) 841, 1584
BsmI GAATGC 1 cut(s) 2047
BsnI GGCC 3 cut(s) 134, 1738, 1861
Bso31I GGTCTC 1 cut(s) 344
BsoBI CYCGRG 2 cut(s) 334, 2100
Bsp1286I GDGCHC 7 cut(s) 181, 1288, 1307, 1605, 1637, 1894, 1978
Bsp143I GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
Bsp68I TCGCGA 1 cut(s) 618
BspACI CCGC 9 cut(s) 40, 137, 163, 171, 268, 659, 662, 1263, 1341
BspANI GGCC 3 cut(s) 134, 1738, 1861
BspCNI CTCAG 4 cut(s) 220, 628, 927, 1819
BspFNI CGCG 4 cut(s) 42, 188, 223, 618
BspLI GGNNCC 4 cut(s) 135, 323, 697, 1153
BspMAI CTGCAG 3 cut(s) 382, 1276, 1360
BspOI GCTAGC 3 cut(s) 426, 1052, 1508
BspPI GGATC 5 cut(s) 205, 321, 567, 1369, 2200
BspQI GCTCTTC 1 cut(s) 201
BspTNI GGTCTC 1 cut(s) 344
BsrBI CCGCTC 1 cut(s) 268
BsrDI GCAATG 4 cut(s) 59, 1122, 1677, 1701
BsrI ACTGG 9 cut(s) 574, 835, 873, 1225, 1249, 1316, 1450, 1915, 2229
BssECI CCNNGG 3 cut(s) 1495, 1562, 1791
BssMI GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
BssNI GRCGYC 3 cut(s) 152, 542, 1065
BssSI CACGAG 2 cut(s) 563, 1893
BssT1I CCWWGG 1 cut(s) 1562
Bst2BI CACGAG 2 cut(s) 563, 1893
Bst2UI CCWGG 2 cut(s) 1496, 2210
Bst4CI ACNGT 2 cut(s) 937, 1705
Bst6I CTCTTC 4 cut(s) 201, 246, 975, 2185
BstACI GRCGYC 3 cut(s) 152, 542, 1065
BstAPI GCANNNNNTGC 4 cut(s) 1271, 1355, 1760, 2259
BstC8I GCNNGC 8 cut(s) 424, 456, 513, 1050, 1108, 1502, 1506, 1740
BstDEI CTNAG 5 cut(s) 207, 636, 914, 1614, 1827
BstDSI CCRYGG 1 cut(s) 1791
BstF5I GGATG 7 cut(s) 490, 670, 739, 1080, 1311, 2070, 2158
BstFNI CGCG 4 cut(s) 42, 188, 223, 618
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 225, 290
BstKTI GATC 8 cut(s) 213, 316, 505, 562, 1364, 1459, 1518, 2208
BstMAI GTCTC 4 cut(s) 304, 344, 1088, 1916
BstMBI GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
BstNI CCWGG 2 cut(s) 1496, 2210
BstSCI CCNGG 3 cut(s) 309, 1494, 2208
BstSFI CTRYAG 3 cut(s) 378, 1272, 1356
BstSLI GKGCMC 1 cut(s) 1307
BstUI CGCG 4 cut(s) 42, 188, 223, 618
BstV2I GAAGAC 3 cut(s) 21, 561, 1554
BstX2I RGATCY 5 cut(s) 210, 313, 1361, 1515, 2205
BstXI CCANNNNNNTGG 2 cut(s) 434, 706
BstYI RGATCY 5 cut(s) 210, 313, 1361, 1515, 2205
BsuRI GGCC 3 cut(s) 134, 1738, 1861
BtgI CCRYGG 1 cut(s) 1791
BtsCI GGATG 7 cut(s) 490, 670, 739, 1080, 1311, 2070, 2158
BtsI GCAGTG 3 cut(s) 1605, 1635, 1635
BtsIMutI CAGTG 5 cut(s) 880, 1605, 1635, 1635, 1922
BtuMI TCGCGA 1 cut(s) 618
Cac8I GCNNGC 8 cut(s) 424, 456, 513, 1050, 1108, 1502, 1506, 1740
CaiI CAGNNNCTG 4 cut(s) 1364, 1760, 1931, 2209
CfoI GCGC 2 cut(s) 225, 290
Cfr13I GGNCC 4 cut(s) 133, 270, 322, 696
CseI GACGC 7 cut(s) 160, 166, 229, 268, 531, 542, 866
Csp6I GTAC 3 cut(s) 166, 996, 2047
CspCI CAANNNNNGTGG 2 cut(s) 1773, 1808
CviAII CATG 8 cut(s) 130, 384, 431, 550, 1574, 1850, 1940, 2254
CviQI GTAC 3 cut(s) 166, 996, 2047
DdeI CTNAG 5 cut(s) 207, 636, 914, 1614, 1827
DpnI GATC 8 cut(s) 212, 315, 504, 561, 1363, 1458, 1517, 2207
DpnII GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
EaeI YGGCCR 1 cut(s) 1736
Eam1104I CTCTTC 4 cut(s) 201, 246, 975, 2185
EarI CTCTTC 4 cut(s) 201, 246, 975, 2185
EciI GGCGGA 1 cut(s) 677
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 1562
Eco147I AGGCCT 1 cut(s) 1861
Eco24I GRGCYC 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 3 cut(s) 270, 322, 696
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 4 cut(s) 98, 380, 474, 480
Eco88I CYCGRG 2 cut(s) 334, 2100
EcoICRI GAGCTC 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 2115
EcoRII CCWGG 2 cut(s) 1494, 2208
EcoT14I CCWWGG 1 cut(s) 1562
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 1562
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 8 cut(s) 133, 387, 434, 553, 1577, 1853, 1943, 2257
FaqI GGGAC 2 cut(s) 841, 1584
FatI CATG 8 cut(s) 129, 383, 430, 549, 1573, 1849, 1939, 2253
FauI CCCGC 2 cut(s) 144, 1270
FblI GTMKAC 4 cut(s) 257, 869, 2092, 2195
FokI GGATG 7 cut(s) 497, 677, 746, 1087, 1298, 2077, 2165
FriOI GRGCYC 1 cut(s) 181
FspBI CTAG 9 cut(s) 423, 546, 1049, 1253, 1289, 1437, 1505, 2013, 2088
GlaI GCGC 2 cut(s) 224, 289
GsuI CTGGAG 6 cut(s) 386, 1242, 1428, 1467, 1898, 2231
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 3 cut(s) 134, 1738, 1861
HapII CCGG 2 cut(s) 174, 311
HgaI GACGC 7 cut(s) 160, 166, 229, 268, 531, 542, 866
HhaI GCGC 2 cut(s) 225, 290
Hin1I GRCGYC 3 cut(s) 152, 542, 1065
Hin1II CATG 8 cut(s) 133, 387, 434, 553, 1577, 1853, 1943, 2257
Hin6I GCGC 2 cut(s) 223, 288
HinP1I GCGC 2 cut(s) 223, 288
HincII GTYRAC 3 cut(s) 258, 906, 1236
HindII GTYRAC 3 cut(s) 258, 906, 1236
HindIII AAGCTT 1 cut(s) 1817
HinfI GANTC 8 cut(s) 254, 293, 374, 566, 803, 1416, 1610, 2171
HpaII CCGG 2 cut(s) 174, 311
HphI GGTGA 3 cut(s) 652, 721, 1152
Hpy188I TCNGA 7 cut(s) 150, 210, 304, 493, 502, 1005, 1804
Hpy99I CGWCG 5 cut(s) 145, 160, 262, 308, 1005
HpyAV CCTTC 3 cut(s) 458, 1142, 2222
HpyCH4III ACNGT 2 cut(s) 937, 1705
HpyCH4IV ACGT 3 cut(s) 34, 976, 1065
HpyF3I CTNAG 5 cut(s) 207, 636, 914, 1614, 1827
HpySE526I ACGT 3 cut(s) 34, 976, 1065
Hsp92I GRCGYC 3 cut(s) 152, 542, 1065
Hsp92II CATG 8 cut(s) 133, 387, 434, 553, 1577, 1853, 1943, 2257
HspAI GCGC 2 cut(s) 223, 288
Kzo9I GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
LguI GCTCTTC 1 cut(s) 201
LweI GCATC 7 cut(s) 131, 475, 601, 1009, 1036, 1400, 2055
MaeI CTAG 9 cut(s) 423, 546, 1049, 1253, 1289, 1437, 1505, 2013, 2088
MaeII ACGT 3 cut(s) 34, 976, 1065
MaeIII GTNAC 8 cut(s) 875, 884, 931, 1013, 1720, 1833, 1864, 2155
MalI GATC 8 cut(s) 212, 315, 504, 561, 1363, 1458, 1517, 2207
MbiI CCGCTC 1 cut(s) 268
MboI GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
MfeI CAATTG 3 cut(s) 809, 1266, 1785
MflI RGATCY 5 cut(s) 210, 313, 1361, 1515, 2205
MhlI GDGCHC 7 cut(s) 181, 1288, 1307, 1605, 1637, 1894, 1978
MlsI TGGCCA 1 cut(s) 1738
MluNI TGGCCA 1 cut(s) 1738
MlyI GAGTC 6 cut(s) 263, 287, 575, 797, 1410, 1619
MmeI TCCRAC 4 cut(s) 327, 415, 983, 1319
Mox20I TGGCCA 1 cut(s) 1738
MscI TGGCCA 1 cut(s) 1738
MseI TTAA 2 cut(s) 852, 1622
MslI CAYNNNNRTG 1 cut(s) 704
Msp20I TGGCCA 1 cut(s) 1738
MspA1I CMGCKG 3 cut(s) 163, 704, 1346
MspI CCGG 2 cut(s) 174, 311
MspR9I CCNGG 3 cut(s) 311, 1496, 2210
MunI CAATTG 3 cut(s) 809, 1266, 1785
Mva1269I GAATGC 1 cut(s) 2047
MvaI CCWGG 2 cut(s) 1496, 2210
MvnI CGCG 4 cut(s) 42, 188, 223, 618
NciI CCSGG 1 cut(s) 311
NdeII GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
NheI GCTAGC 3 cut(s) 422, 1048, 1504
NlaIII CATG 8 cut(s) 133, 387, 434, 553, 1577, 1853, 1943, 2257
NlaIV GGNNCC 4 cut(s) 135, 323, 697, 1153
NmeAIII GCCGAG 1 cut(s) 1003
NmuCI GTSAC 2 cut(s) 875, 884
NruI TCGCGA 1 cut(s) 618
OliI CACNNNNGTG 1 cut(s) 704
PaeR7I CTCGAG 1 cut(s) 2100
PceI AGGCCT 1 cut(s) 1861
PciSI GCTCTTC 1 cut(s) 201
PctI GAATGC 1 cut(s) 2047
PfeI GAWTC 2 cut(s) 374, 2171
PflFI GACNNNGTC 1 cut(s) 296
PflMI CCANNNNNTGG 2 cut(s) 1322, 1921
PfoI TCCNGGA 1 cut(s) 2208
PleI GAGTC 6 cut(s) 262, 287, 574, 797, 1410, 1618
PpsI GAGTC 6 cut(s) 262, 287, 574, 797, 1410, 1618
Psp124BI GAGCTC 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 1494, 2208
PspGI CCWGG 2 cut(s) 1494, 2208
PspN4I GGNNCC 4 cut(s) 135, 323, 697, 1153
PspPI GGNCC 4 cut(s) 133, 270, 322, 696
PstI CTGCAG 3 cut(s) 382, 1276, 1360
PstNI CAGNNNCTG 4 cut(s) 1364, 1760, 1931, 2209
PsuI RGATCY 5 cut(s) 210, 313, 1361, 1515, 2205
PsyI GACNNNGTC 1 cut(s) 296
PvuII CAGCTG 2 cut(s) 704, 1346
RruI TCGCGA 1 cut(s) 618
RsaI GTAC 3 cut(s) 167, 997, 2048
RsaNI GTAC 3 cut(s) 166, 996, 2047
RseI CAYNNNNRTG 1 cut(s) 704
SacI GAGCTC 1 cut(s) 181
SalI GTCGAC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 2 cut(s) 852, 1622
Sau3AI GATC 8 cut(s) 210, 313, 502, 559, 1361, 1456, 1515, 2205
Sau96I GGNCC 4 cut(s) 133, 270, 322, 696
SchI GAGTC 6 cut(s) 263, 287, 575, 797, 1410, 1619
ScrFI CCNGG 3 cut(s) 311, 1496, 2210
SduI GDGCHC 7 cut(s) 181, 1288, 1307, 1605, 1637, 1894, 1978
SfaNI GCATC 7 cut(s) 131, 475, 601, 1009, 1036, 1400, 2055
SfcI CTRYAG 3 cut(s) 378, 1272, 1356
Sfr274I CTCGAG 1 cut(s) 2100
SinI GGWCC 3 cut(s) 270, 322, 696
SlaI CTCGAG 1 cut(s) 2100
SmiMI CAYNNNNRTG 1 cut(s) 704
SmlI CTYRAG 2 cut(s) 445, 2100
SmoI CTYRAG 2 cut(s) 445, 2100
SseBI AGGCCT 1 cut(s) 1861
SsiI CCGC 9 cut(s) 40, 137, 163, 171, 268, 659, 662, 1263, 1341
SspMI CTAG 9 cut(s) 423, 546, 1049, 1253, 1289, 1437, 1505, 2013, 2088
SstI GAGCTC 1 cut(s) 181
StuI AGGCCT 1 cut(s) 1861
StyD4I CCNGG 3 cut(s) 309, 1494, 2208
StyI CCWWGG 1 cut(s) 1562
TaaI ACNGT 2 cut(s) 937, 1705
TaiI ACGT 3 cut(s) 37, 979, 1068
TaqI TCGA 8 cut(s) 18, 107, 257, 578, 1455, 1512, 1985, 2101
TauI GCSGC 4 cut(s) 42, 173, 662, 1343
TfiI GAWTC 2 cut(s) 374, 2171
Tru1I TTAA 2 cut(s) 852, 1622
Tru9I TTAA 2 cut(s) 852, 1622
TscAI CASTG 5 cut(s) 880, 1605, 1635, 1642, 1922
TseFI GTSAC 2 cut(s) 875, 884
Tsp45I GTSAC 2 cut(s) 875, 884
TspDTI ATGAA 9 cut(s) 92, 188, 618, 624, 960, 1091, 1571, 1697, 1838
TspGWI ACGGA 4 cut(s) 275, 286, 2072, 2174
TspRI CASTG 5 cut(s) 880, 1605, 1635, 1642, 1922
Tth111I GACNNNGTC 1 cut(s) 296
Van91I CCANNNNNTGG 2 cut(s) 1322, 1921
VpaK11BI GGWCC 3 cut(s) 270, 322, 696
XapI RAATTY 5 cut(s) 74, 93, 496, 1618, 2115
XcmI CCANNNNNNNNNTGG 2 cut(s) 1096, 1170
XhoI CTCGAG 1 cut(s) 2100
XmiI GTMKAC 4 cut(s) 257, 869, 2092, 2195
XspI CTAG 9 cut(s) 423, 546, 1049, 1253, 1289, 1437, 1505, 2013, 2088
ZraI GACGTC 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.