Rh2BG136600

Transmembrane and coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008389
Physical Location & Seq
Forward (+)
11622920 .. 11628079
5160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG136600.1

Sequence Viewer

Length: 1473 bp
ATGTCCACGACGACGTCGTTCTTAACGCCGACGCAGCGGTACGGCGCCGGAGCTTTGTTCGCTTTCGCTCTCCACCAGGCTCAGATCCACCAGACTCGCCCCTTGGGGTTGCTTACTCACGAAGACTCAACCGCCGCCGACCGAGCCAGTAGCTGCAGCAGTTCCGACTCCGTCTCCGATGACCCGTTGCTTTGGGTCAATGAAAACTCCGGCCTCCTTCCACCCGTGTTTAGGTTTCTAGAGATTGATCCTGCAGCATGGTCTGGACTTCAAGAAACTGCTGCTGCTTCTCCGGCTAGCAATCATGTTGGAGCATTCTTGAGGTTGCTGTCAGAAGAAGGTGATGGCAAAAATGCTTCGGAAAATTCAGATCAAGAACTTGCTTTGTCAAGAGCTGTTGATGCTATGGCGCGTAGCATGGAACAGGATTCCGAGTCTTCTAAGTCTAAGAGGGAAAAACATATTGAATACGAACATGAATGCCGTGAGAAATTATCCACACCGGAGGTGAAGCAAAATGCTGAAGCAGCGGATGCGCCTTTGGAAAATTCGCAGGACAAAAGCGTCACAGCCGAGGTGCAACAGATCGTTCAAGCAACGGAGGTGCCTTTGGAAAATCAGCGAGAGATTCGTGGTACTATGGTTAATGTTAAAGAGACAGTTGATAAGTCCAAGAGTGACATTGATGAGCAACCTGTTGTGGAGGCAAGAATGCTCAGTAATGAGAGGAAAATTACAGTTCTGTATGAGATTCTTTCTGCTTGTCTGGCTGATAGGCGTGAAGACAACAAAAAGTCTACCCGTCGGAGAAAGGGTTATGATGCTCGGCATCGTGTAGCTTTGCGCTTACTAGCAACTTGGCTTGATGTCAAGTGGATACAAATGGAAGCTATTGAGACAATGGTTGCTTGCTCTGCAATGGCTCTATTGAAAAATGAAGAAGAAAAAGAAGAAACTCAGTCACCAAAAAGCAAATGGGCTAAATGGAAGCGTCGTGGTATCGTGGGTGCAGCTGCAGTAACTGGAGGAGTGCTGCTGACAGTAACTGGTGGATTAGCTGCCCCAGCAATTGCCGCAGGACTTGGTGCCCTAGCTCCAACACTGGGCACTATCATCCCAGTAATTGGAGCAACTGGTTTTGCTGCGGCCGCAACTGCTGCTGGTTCTGTTGCTGGTTCCATTGTTGTTGCTGCATCATTTGGAGCTGCTGGAGCTGGACTGACAGGGTTCAAAATGGCTAGAAGAACAGGAGGTGTTGATGAATTTGAGTTCAAAGCTATTGGAGAAAATCACAACCAAGGCAGACTAGCAGTTGAGATCTTGGTCTCAGGATTTGTTTTCGATGTGGAAGATTTTGTGAGGCCTTGGGAGGGACAGAATGGAAATTTGGAAAGGTATGCCGTGCTCTGGGAGTCTAAGAATCTAATTGCAGTGAGCACTGCCATTCAGGACTGGCTTACTTCAAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF726

Protein Analysis

490

Amino Acids

52.87

Weight (kDa)

5.25

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF726 PF05277 328 - 489 1.5e-29 Protein of unknown function (DUF726)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 563
AatII GACGTC 1 cut(s) 17
AccB1I GGYRCC 3 cut(s) 44, 604, 1085
AccB7I CCANNNNNTGG 1 cut(s) 1124
AccI GTMKAC 1 cut(s) 797
AccII CGCG 1 cut(s) 412
AciI CCGC 7 cut(s) 37, 132, 135, 530, 1074, 1145, 1149
AclWI GGATC 2 cut(s) 79, 242
AcoI YGGCCR 1 cut(s) 1146
AcsI RAATTY 4 cut(s) 364, 547, 1262, 1384
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 2 cut(s) 14, 45
AfaI GTAC 2 cut(s) 41, 637
AfiI CCNNNNNNNGG 5 cut(s) 231, 1103, 1124, 1370, 1407
AgsI TTSAA 7 cut(s) 272, 467, 593, 931, 1231, 1273, 1464
AjnI CCWGG 1 cut(s) 75
AjuI GAANNNNNNNTTGG 2 cut(s) 1370, 1402
Alw21I GWGCWC 2 cut(s) 1407, 1439
Alw26I GTCTC 4 cut(s) 178, 650, 890, 1330
AlwI GGATC 2 cut(s) 79, 242
AlwNI CAGNNNCTG 3 cut(s) 153, 1022, 1046
AoxI GGCC 3 cut(s) 211, 1146, 1361
ApoI RAATTY 4 cut(s) 364, 547, 1262, 1384
AspLEI GCGC 4 cut(s) 47, 412, 538, 846
AsuHPI GGTGA 3 cut(s) 353, 520, 954
AsuNHI GCTAGC 1 cut(s) 296
BaeGI GKGCMC 2 cut(s) 1090, 1109
BaeI ACNNNNGTAYC 2 cut(s) 627, 660
BanI GGYRCC 3 cut(s) 44, 604, 1085
BbsI GAAGAC 3 cut(s) 129, 429, 789
Bbv12I GWGCWC 2 cut(s) 1407, 1439
BccI CCATC 1 cut(s) 338
BceAI ACGGC 3 cut(s) 58, 468, 1385
BciT130I CCWGG 1 cut(s) 77
BciVI GTATCC 1 cut(s) 870
BcoDI GTCTC 4 cut(s) 178, 650, 890, 1330
BfaI CTAG 6 cut(s) 239, 297, 851, 1091, 1239, 1307
BfmI CTRYAG 3 cut(s) 154, 252, 1014
BfoI RGCGCY 1 cut(s) 48
BfuI GTATCC 1 cut(s) 870
BglII AGATCT 1 cut(s) 1317
Bme1390I CCNGG 1 cut(s) 77
BmiI GGNNCC 4 cut(s) 46, 606, 1087, 1177
BmrFI CCNGG 1 cut(s) 77
BmrI ACTGGG 2 cut(s) 1112, 1112
BmsI GCATC 5 cut(s) 391, 523, 811, 838, 1202
BmtI GCTAGC 1 cut(s) 300
BmuI ACTGGG 2 cut(s) 1112, 1112
BpiI GAAGAC 3 cut(s) 129, 429, 789
BpmI CTGGAG 2 cut(s) 1044, 1230
BpuEI CTTGAG 1 cut(s) 340
BsaHI GRCGYC 2 cut(s) 14, 45
BsaI GGTCTC 1 cut(s) 1330
BsaJI CCNNGG 4 cut(s) 102, 573, 1297, 1364
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 799, 829
Bsc4I CCNNNNNNNGG 5 cut(s) 231, 1103, 1124, 1370, 1407
Bse1I ACTGG 7 cut(s) 147, 1027, 1051, 1107, 1118, 1138, 1457
Bse3DI GCAATG 1 cut(s) 924
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 102, 573, 1297, 1364
BseGI GGATG 2 cut(s) 538, 1113
BseLI CCNNNNNNNGG 5 cut(s) 231, 1103, 1124, 1370, 1407
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 4 cut(s) 95, 730, 971, 1341
BseNI ACTGG 7 cut(s) 147, 1027, 1051, 1107, 1118, 1138, 1457
BseRI GAGGAG 1 cut(s) 1041
BseSI GKGCMC 2 cut(s) 1090, 1109
BseX3I CGGCCG 1 cut(s) 1146
BseYI CCCAGC 1 cut(s) 1063
BsgI GTGCAG 1 cut(s) 1029
Bsh1236I CGCG 1 cut(s) 412
Bsh1285I CGRYCG 2 cut(s) 142, 1149
BshFI GGCC 3 cut(s) 213, 1148, 1363
BshNI GGYRCC 3 cut(s) 44, 604, 1085
BsiEI CGRYCG 2 cut(s) 142, 1149
BsiHKAI GWGCWC 2 cut(s) 1407, 1439
BsiSI CCGG 4 cut(s) 48, 210, 293, 503
BslFI GGGAC 1 cut(s) 1386
BslI CCNNNNNNNGG 5 cut(s) 231, 1103, 1124, 1370, 1407
BsmAI GTCTC 4 cut(s) 178, 650, 890, 1330
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 1386
BsmI GAATGC 3 cut(s) 314, 485, 717
BsnI GGCC 3 cut(s) 213, 1148, 1363
Bso31I GGTCTC 1 cut(s) 1330
Bsp1286I GDGCHC 4 cut(s) 1090, 1109, 1407, 1439
Bsp143I GATC 5 cut(s) 84, 247, 370, 585, 1317
BspACI CCGC 7 cut(s) 37, 132, 135, 530, 1074, 1145, 1149
BspANI GGCC 3 cut(s) 213, 1148, 1363
BspCNI CTCAG 4 cut(s) 94, 729, 970, 1340
BspFNI CGCG 1 cut(s) 412
BspLI GGNNCC 4 cut(s) 46, 606, 1087, 1177
BspMAI CTGCAG 3 cut(s) 158, 256, 1018
BspOI GCTAGC 1 cut(s) 300
BspPI GGATC 2 cut(s) 79, 242
BspT107I GGYRCC 3 cut(s) 44, 604, 1085
BspTNI GGTCTC 1 cut(s) 1330
BsrDI GCAATG 1 cut(s) 924
BsrI ACTGG 7 cut(s) 147, 1027, 1051, 1107, 1118, 1138, 1457
BssECI CCNNGG 4 cut(s) 102, 573, 1297, 1364
BssMI GATC 5 cut(s) 84, 247, 370, 585, 1317
BssNI GRCGYC 2 cut(s) 14, 45
BssT1I CCWWGG 3 cut(s) 102, 1297, 1364
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 3 cut(s) 661, 739, 1042
BstACI GRCGYC 2 cut(s) 14, 45
BstAPI GCANNNNNTGC 2 cut(s) 533, 1157
BstC8I GCNNGC 2 cut(s) 298, 910
BstDEI CTNAG 7 cut(s) 81, 441, 447, 716, 957, 1327, 1416
BstF5I GGATG 2 cut(s) 538, 1113
BstFNI CGCG 1 cut(s) 412
BstH2I RGCGCY 1 cut(s) 48
BstHHI GCGC 4 cut(s) 47, 412, 538, 846
BstKTI GATC 5 cut(s) 87, 250, 373, 588, 1320
BstMAI GTCTC 4 cut(s) 178, 650, 890, 1330
BstMBI GATC 5 cut(s) 84, 247, 370, 585, 1317
BstMCI CGRYCG 2 cut(s) 142, 1149
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstSFI CTRYAG 3 cut(s) 154, 252, 1014
BstSLI GKGCMC 2 cut(s) 1090, 1109
BstUI CGCG 1 cut(s) 412
BstV2I GAAGAC 3 cut(s) 129, 429, 789
BstX2I RGATCY 2 cut(s) 84, 1317
BstYI RGATCY 2 cut(s) 84, 1317
BstZI CGGCCG 1 cut(s) 1146
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 3 cut(s) 213, 1148, 1363
BtsCI GGATG 2 cut(s) 538, 1113
BtsI GCAGTG 2 cut(s) 1437, 1437
BtsIMutI CAGTG 3 cut(s) 1100, 1437, 1437
Cac8I GCNNGC 2 cut(s) 298, 910
CaiI CAGNNNCTG 3 cut(s) 153, 1022, 1046
CciNI GCGGCCGC 1 cut(s) 1146
CfoI GCGC 4 cut(s) 47, 412, 538, 846
CseI GACGC 3 cut(s) 40, 553, 980
Csp6I GTAC 2 cut(s) 40, 636
CviAII CATG 4 cut(s) 258, 305, 418, 476
CviQI GTAC 2 cut(s) 40, 636
DdeI CTNAG 7 cut(s) 81, 441, 447, 716, 957, 1327, 1416
DinI GGCGCC 1 cut(s) 46
DpnI GATC 5 cut(s) 86, 249, 372, 587, 1319
DpnII GATC 5 cut(s) 84, 247, 370, 585, 1317
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
EaeI YGGCCR 1 cut(s) 1146
EagI CGGCCG 1 cut(s) 1146
EclXI CGGCCG 1 cut(s) 1146
Eco130I CCWWGG 3 cut(s) 102, 1297, 1364
Eco147I AGGCCT 1 cut(s) 1363
Eco31I GGTCTC 1 cut(s) 1330
Eco52I CGGCCG 1 cut(s) 1146
Eco57I CTGAAG 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 3 cut(s) 102, 1297, 1364
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
ErhI CCWWGG 3 cut(s) 102, 1297, 1364
Esp3I CGTCTC 1 cut(s) 178
FaeI CATG 4 cut(s) 261, 308, 421, 479
FaqI GGGAC 1 cut(s) 1386
FatI CATG 4 cut(s) 257, 304, 417, 475
FblI GTMKAC 1 cut(s) 797
FokI GGATG 2 cut(s) 545, 1100
FspBI CTAG 6 cut(s) 239, 297, 851, 1091, 1239, 1307
GlaI GCGC 4 cut(s) 46, 411, 537, 845
GsaI CCCAGC 1 cut(s) 1067
GsuI CTGGAG 2 cut(s) 1044, 1230
HaeII RGCGCY 1 cut(s) 48
HaeIII GGCC 3 cut(s) 213, 1148, 1363
HapII CCGG 4 cut(s) 48, 210, 293, 503
HgaI GACGC 3 cut(s) 40, 553, 980
HhaI GCGC 4 cut(s) 47, 412, 538, 846
Hin1I GRCGYC 2 cut(s) 14, 45
Hin1II CATG 4 cut(s) 261, 308, 421, 479
Hin6I GCGC 4 cut(s) 45, 410, 536, 844
HinP1I GCGC 4 cut(s) 45, 410, 536, 844
HinfI GANTC 9 cut(s) 94, 125, 167, 428, 434, 628, 751, 1412, 1420
HpaII CCGG 4 cut(s) 48, 210, 293, 503
HphI GGTGA 3 cut(s) 353, 520, 954
Hpy166II GTNNAC 2 cut(s) 6, 798
Hpy188I TCNGA 8 cut(s) 84, 166, 178, 334, 361, 370, 433, 807
Hpy188III TCNNGA 9 cut(s) 119, 239, 264, 272, 319, 374, 390, 1329, 1448
Hpy8I GTNNAC 2 cut(s) 6, 798
Hpy99I CGWCG 6 cut(s) 13, 16, 19, 34, 807, 996
HpyAV CCTTC 2 cut(s) 227, 332
HpyCH4III ACNGT 3 cut(s) 661, 739, 1042
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 8 cut(s) 156, 254, 580, 917, 1010, 1016, 1193, 1430
HpyF3I CTNAG 7 cut(s) 81, 441, 447, 716, 957, 1327, 1416
HpySE526I ACGT 1 cut(s) 14
Hsp92I GRCGYC 2 cut(s) 14, 45
Hsp92II CATG 4 cut(s) 261, 308, 421, 479
HspAI GCGC 4 cut(s) 45, 410, 536, 844
KasI GGCGCC 1 cut(s) 44
Kzo9I GATC 5 cut(s) 84, 247, 370, 585, 1317
LmnI GCTCC 6 cut(s) 50, 311, 1099, 1127, 1202, 1211
LweI GCATC 5 cut(s) 391, 523, 811, 838, 1202
MaeI CTAG 6 cut(s) 239, 297, 851, 1091, 1239, 1307
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 5 cut(s) 565, 677, 960, 1018, 1042
MalI GATC 5 cut(s) 86, 249, 372, 587, 1319
MboI GATC 5 cut(s) 84, 247, 370, 585, 1317
MboII GAAGA 9 cut(s) 134, 347, 429, 794, 950, 953, 962, 1254, 1361
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 2 cut(s) 84, 1317
MhlI GDGCHC 4 cut(s) 1090, 1109, 1407, 1439
Mly113I GGCGCC 1 cut(s) 45
MlyI GAGTC 5 cut(s) 88, 119, 161, 443, 1421
MmeI TCCRAC 4 cut(s) 189, 289, 785, 1121
MseI TTAA 3 cut(s) 23, 645, 651
MspA1I CMGCKG 3 cut(s) 37, 530, 1013
MspI CCGG 4 cut(s) 48, 210, 293, 503
MspR9I CCNGG 1 cut(s) 77
MunI CAATTG 1 cut(s) 1068
Mva1269I GAATGC 3 cut(s) 314, 485, 717
MvaI CCWGG 1 cut(s) 77
MvnI CGCG 1 cut(s) 412
NarI GGCGCC 1 cut(s) 45
NdeII GATC 5 cut(s) 84, 247, 370, 585, 1317
NheI GCTAGC 1 cut(s) 296
NlaIII CATG 4 cut(s) 261, 308, 421, 479
NlaIV GGNNCC 4 cut(s) 46, 606, 1087, 1177
NmeAIII GCCGAG 2 cut(s) 598, 805
NmuCI GTSAC 3 cut(s) 565, 677, 960
NotI GCGGCCGC 1 cut(s) 1146
PceI AGGCCT 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 3 cut(s) 314, 485, 717
PfeI GAWTC 4 cut(s) 428, 628, 751, 1420
PflFI GACNNNGTC 2 cut(s) 13, 170
PflMI CCANNNNNTGG 1 cut(s) 1124
PleI GAGTC 5 cut(s) 88, 119, 161, 442, 1420
PluTI GGCGCC 1 cut(s) 48
PpsI GAGTC 5 cut(s) 88, 119, 161, 442, 1420
Psp6I CCWGG 1 cut(s) 75
PspFI CCCAGC 1 cut(s) 1063
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 4 cut(s) 46, 606, 1087, 1177
PstI CTGCAG 3 cut(s) 158, 256, 1018
PstNI CAGNNNCTG 3 cut(s) 153, 1022, 1046
PsuI RGATCY 2 cut(s) 84, 1317
PsyI GACNNNGTC 2 cut(s) 13, 170
PvuII CAGCTG 1 cut(s) 1013
RsaI GTAC 2 cut(s) 41, 637
RsaNI GTAC 2 cut(s) 40, 636
SaqAI TTAA 3 cut(s) 23, 645, 651
Sau3AI GATC 5 cut(s) 84, 247, 370, 585, 1317
SchI GAGTC 5 cut(s) 88, 119, 161, 443, 1421
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 4 cut(s) 1090, 1109, 1407, 1439
SfaNI GCATC 5 cut(s) 391, 523, 811, 838, 1202
SfcI CTRYAG 3 cut(s) 154, 252, 1014
SfoI GGCGCC 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
SseBI AGGCCT 1 cut(s) 1363
SsiI CCGC 7 cut(s) 37, 132, 135, 530, 1074, 1145, 1149
SspDI GGCGCC 1 cut(s) 44
SspMI CTAG 6 cut(s) 239, 297, 851, 1091, 1239, 1307
StuI AGGCCT 1 cut(s) 1363
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 3 cut(s) 102, 1297, 1364
TaaI ACNGT 3 cut(s) 661, 739, 1042
TaiI ACGT 1 cut(s) 17
TaqI TCGA 1 cut(s) 1341
TaqII GACCGA 1 cut(s) 156
TauI GCSGC 4 cut(s) 137, 1076, 1148, 1151
TfiI GAWTC 4 cut(s) 428, 628, 751, 1420
Tru1I TTAA 3 cut(s) 23, 645, 651
Tru9I TTAA 3 cut(s) 23, 645, 651
TscAI CASTG 3 cut(s) 1107, 1437, 1444
TseFI GTSAC 3 cut(s) 565, 677, 960
Tsp45I GTSAC 3 cut(s) 565, 677, 960
TspDTI ATGAA 4 cut(s) 216, 492, 951, 1275
TspGWI ACGGA 2 cut(s) 160, 614
TspRI CASTG 3 cut(s) 1107, 1437, 1444
Tth111I GACNNNGTC 2 cut(s) 13, 170
Van91I CCANNNNNTGG 1 cut(s) 1124
XapI RAATTY 4 cut(s) 364, 547, 1262, 1384
XbaI TCTAGA 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 1 cut(s) 972
XmiI GTMKAC 1 cut(s) 797
XspI CTAG 6 cut(s) 239, 297, 851, 1091, 1239, 1307
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.