MD15G1278200.v1.1

Mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0018491
Physical Location & Seq
Reverse (-)
24570936 .. 24583748
12813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1278200.v1.1.491

Sequence Viewer

Length: 402 bp
ATGCTGCCTGAGGAGGGCTTCGCTCTCAGAGAATGGAGGAGTTCTTGGAGTGAATCTCCTTATAAGGATGCTGCAAAGAGAACGGCATCTGGGAATTTACCTCTTAATGCATTTTTGTCTGAGTGGGCCCTCATGACACTGTTAAATCCAAAGCGAAGTTTGGCGAATTTGATATATGTTGGATACAATGGCAGTCCTGCTTCAGCTATCCATGTTACTAGAAGAAGATCAGTAGATCATAAAAGCCAAAAAACAAAAAGAAATGTTTTCAGCTGCTTCATTTTTGGTCCTAAAAATGCAGGAAAATCCGCTATTTTAAATTCATTCATAGGAAGTAATAGGTGGAGGCATCGACTAATCATCTGGCTGATCAGCAGATGCCAGTTTATAATCTTGCATTGA

Protein Analysis

134

Amino Acids

15.22

Weight (kDa)

10.76

Isoelectric Point (pI)

65.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF_assoc_1 PF08355 16 - 84 7.5e-23 EF hand associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 63, 389
AciI CCGC 1 cut(s) 309
AcsI RAATTY 3 cut(s) 94, 166, 319
AcuI CTGAAG 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 14
AluBI AGCT 2 cut(s) 206, 273
AluI AGCT 2 cut(s) 206, 273
AoxI GGCC 1 cut(s) 126
ApaI GGGCCC 1 cut(s) 130
ApeKI GCWGC 3 cut(s) 4, 71, 273
ApoI RAATTY 3 cut(s) 94, 166, 319
AspS9I GGNCC 3 cut(s) 126, 127, 287
AvaII GGWCC 1 cut(s) 287
AxyI CCTNAGG 1 cut(s) 9
BaeGI GKGCMC 1 cut(s) 130
BanII GRGCYC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 58, 260
BceAI ACGGC 1 cut(s) 99
BciVI GTATCC 1 cut(s) 176
BclI TGATCA 1 cut(s) 369
BfaI CTAG 1 cut(s) 219
BfuI GTATCC 1 cut(s) 176
BisI GCNGC 3 cut(s) 5, 72, 274
BlsI GCNGC 3 cut(s) 6, 73, 275
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 126, 127, 287
BmiI GGNNCC 1 cut(s) 128
BmsI GCATC 4 cut(s) 58, 95, 358, 368
BplI GAGNNNNNCTC 2 cut(s) 40, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 382
Bse21I CCTNAGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 14
BseMII CTCAG 2 cut(s) 40, 111
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 2 cut(s) 26, 52
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 2 cut(s) 58, 260
BshFI GGCC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 14
BsnI GGCC 1 cut(s) 128
Bsp120I GGGCCC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 3 cut(s) 227, 235, 369
BspACI CCGC 1 cut(s) 309
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 2 cut(s) 39, 112
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 382
BssMI GATC 3 cut(s) 227, 235, 369
Bst4CI ACNGT 1 cut(s) 141
BstDEI CTNAG 3 cut(s) 9, 26, 120
BstF5I GGATG 1 cut(s) 73
BstKTI GATC 3 cut(s) 230, 238, 372
BstMBI GATC 3 cut(s) 227, 235, 369
BstSLI GKGCMC 1 cut(s) 130
BstV1I GCAGC 2 cut(s) 58, 260
Bsu36I CCTNAGG 1 cut(s) 9
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 1 cut(s) 128
BtsCI GGATG 1 cut(s) 73
BtsIMutI CAGTG 1 cut(s) 137
CciI TCATGA 1 cut(s) 132
Cfr13I GGNCC 3 cut(s) 126, 127, 287
CviAII CATG 2 cut(s) 133, 212
CviJI RGCY 6 cut(s) 18, 128, 206, 246, 273, 367
CviKI_1 RGCY 6 cut(s) 18, 128, 206, 246, 273, 367
DdeI CTNAG 3 cut(s) 9, 26, 120
DpnI GATC 3 cut(s) 229, 237, 371
DpnII GATC 3 cut(s) 227, 235, 369
DraI TTTAAA 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 186
Eco81I CCTNAGG 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 127
EcoT22I ATGCAT 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 130
FaeI CATG 2 cut(s) 136, 215
FaiI YATR 8 cut(s) 63, 134, 175, 177, 213, 240, 329, 389
FatI CATG 2 cut(s) 132, 211
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 5, 72, 274
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 3 cut(s) 5, 72, 274
FspBI CTAG 1 cut(s) 219
GluI GCNGC 3 cut(s) 5, 72, 274
HaeIII GGCC 1 cut(s) 128
Hin1II CATG 2 cut(s) 136, 215
HinfI GANTC 1 cut(s) 53
Hpy188I TCNGA 2 cut(s) 29, 121
Hpy188III TCNNGA 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 141
HpyCH4V TGCA 4 cut(s) 74, 110, 299, 397
HpyF3I CTNAG 3 cut(s) 9, 26, 120
Hsp92II CATG 2 cut(s) 136, 215
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 3 cut(s) 227, 235, 369
LpnPI CCDG 6 cut(s) 21, 75, 210, 285, 349, 395
Lsp1109I GCAGC 2 cut(s) 58, 260
LweI GCATC 4 cut(s) 58, 95, 358, 368
MaeI CTAG 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 214
MalI GATC 3 cut(s) 229, 237, 371
MboI GATC 3 cut(s) 227, 235, 369
MboII GAAGA 2 cut(s) 234, 237
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 3 cut(s) 94, 166, 319
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 6 cut(s) 4, 7, 30, 111, 140, 339
Mph1103I ATGCAT 1 cut(s) 112
MseI TTAA 3 cut(s) 105, 143, 317
MspA1I CMGCKG 1 cut(s) 273
NdeII GATC 3 cut(s) 227, 235, 369
NlaIII CATG 2 cut(s) 136, 215
NlaIV GGNNCC 1 cut(s) 128
NsiI ATGCAT 1 cut(s) 112
PagI TCATGA 1 cut(s) 132
PfeI GAWTC 1 cut(s) 53
PkrI GCNGC 3 cut(s) 6, 73, 275
PsiI TTATAA 2 cut(s) 63, 389
PspN4I GGNNCC 1 cut(s) 128
PspOMI GGGCCC 1 cut(s) 126
PspPI GGNCC 3 cut(s) 126, 127, 287
PvuII CAGCTG 1 cut(s) 273
SaqAI TTAA 3 cut(s) 105, 143, 317
SatI GCNGC 3 cut(s) 5, 72, 274
Sau3AI GATC 3 cut(s) 227, 235, 369
Sau96I GGNCC 3 cut(s) 126, 127, 287
SduI GDGCHC 1 cut(s) 130
SetI ASST 4 cut(s) 103, 208, 275, 344
SfaNI GCATC 4 cut(s) 58, 95, 358, 368
SinI GGWCC 1 cut(s) 287
Sse9I AATT 3 cut(s) 94, 166, 319
SsiI CCGC 1 cut(s) 309
SspMI CTAG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 141
TaqI TCGA 1 cut(s) 352
TasI AATT 3 cut(s) 94, 166, 319
TfiI GAWTC 1 cut(s) 53
Tru1I TTAA 3 cut(s) 105, 143, 317
Tru9I TTAA 3 cut(s) 105, 143, 317
TscAI CASTG 1 cut(s) 144
TseI GCWGC 3 cut(s) 4, 71, 273
TspDTI ATGAA 3 cut(s) 268, 312, 316
TspRI CASTG 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 3 cut(s) 94, 166, 319
XspI CTAG 1 cut(s) 219
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.