pycom05g12130

GTPase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0018491
Physical Location & Seq
Forward (+)
15601465 .. 15602127
663 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12130.1

Sequence Viewer

Length: 375 bp
ATGCCCCTGGTATGGATTGACTTGGAAATGGCTGGTGAGGGTAAAATAATTCCTTGGAGTGAAGCTCCTTATAAGGATGCTGCAGGGAGAACGGCATCTGGGAATTTACCTCTTAATGCATTTTTGTCTGAGTGGGCCCTCATGACACTGTTAAATCCAAACCGAAGTTTGGCGAATTTGATATATGTTGGATACAATGGCAGTCCTGCTTCAGCTATCCATGTTACTAGAAGAAGATCAGTAGATCGTAAAACGCAAAAAACAGAAAGAAATGTTAGCTGCTTCGTTTTTGGTCCTAAAAATGCAGGAAAATCCCCTATTTTAAATTCATTCATAGGAAGGCATGGCTTTCCCCCTTCATTATACTCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000001 GO:0000166 GO:0000226 GO:0000287 GO:0001505 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005509 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005783 GO:0005829 GO:0005886 GO:0006464 GO:0006508 GO:0006807 GO:0006810 GO:0006839 GO:0006915 GO:0006928 GO:0006996 GO:0007005 GO:0007006 GO:0007010 GO:0007017 GO:0007018 GO:0007154 GO:0007165 GO:0008088 GO:0008150 GO:0008152 GO:0008219 GO:0008637 GO:0009966 GO:0009987 GO:0010570 GO:0010646 GO:0010821 GO:0010970 GO:0012501 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016192 GO:0016462 GO:0016579 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0019725 GO:0019867 GO:0019896 GO:0022406 GO:0023051 GO:0023052 GO:0030705 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032386 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032592 GO:0032865 GO:0032879 GO:0032886 GO:0032991 GO:0033043 GO:0034640 GO:0034643 GO:0035639 GO:0035794 GO:0036094 GO:0036211 GO:0040008 GO:0042592 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044232 GO:0044233 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044432 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0046872 GO:0046902 GO:0046907 GO:0046928 GO:0047497 GO:0048308 GO:0048311 GO:0048489 GO:0048583 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050804 GO:0050896 GO:0051046 GO:0051049 GO:0051056 GO:0051128 GO:0051179 GO:0051234 GO:0051270 GO:0051588 GO:0051640 GO:0051641 GO:0051646 GO:0051648 GO:0051649 GO:0051650 GO:0051654 GO:0051656 GO:0051716 GO:0055081 GO:0055088 GO:0055091 GO:0060237 GO:0060341 GO:0060632 GO:0061024 GO:0065007 GO:0065008 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0072384 GO:0090559 GO:0097159 GO:0097190 GO:0097345 GO:0097367 GO:0097479 GO:0097480 GO:0098573 GO:0098588 GO:0098796 GO:0098798 GO:0098799 GO:0098805 GO:0098930 GO:0099003 GO:0099098 GO:0099111 GO:0099177 GO:0140056 GO:1900428 GO:1901265 GO:1901363 GO:1901564 GO:1902108 GO:1902110 GO:1902513 GO:1902531 GO:1902686 GO:1903338 GO:1903530 GO:1905710 GO:1990456
KEGG Pathways
Metabolic & Signaling
ko04137 ko04214 map04137 map04214
Pfam Domains
Protein Families
EF-hand_5 EF_assoc_1 EF_assoc_2 Ras

Protein Analysis

125

Amino Acids

13.62

Weight (kDa)

9.84

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF_assoc_1 PF08355 19 - 87 3e-22 EF hand associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
AcsI RAATTY 3 cut(s) 103, 175, 325
AcuI CTGAAG 1 cut(s) 195
AfiI CCNNNNNNNGG 2 cut(s) 12, 169
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 3 cut(s) 65, 215, 279
AluI AGCT 3 cut(s) 65, 215, 279
AoxI GGCC 1 cut(s) 135
ApaI GGGCCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 80, 279
ApoI RAATTY 3 cut(s) 103, 175, 325
AspS9I GGNCC 3 cut(s) 135, 136, 293
AsuHPI GGTGA 1 cut(s) 47
AvaII GGWCC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 67, 266
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 1 cut(s) 8
BciVI GTATCC 1 cut(s) 185
BfaI CTAG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 81
BfuI GTATCC 1 cut(s) 185
BisI GCNGC 2 cut(s) 81, 280
BlsI GCNGC 2 cut(s) 82, 281
Bme1390I CCNGG 1 cut(s) 8
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 3 cut(s) 135, 136, 293
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 8
BmsI GCATC 2 cut(s) 67, 104
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BsaJI CCNNGG 2 cut(s) 6, 53
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 169
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 2 cut(s) 6, 53
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 2 cut(s) 12, 169
BseMII CTCAG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 2 cut(s) 67, 266
BshFI GGCC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 12, 169
BsnI GGCC 1 cut(s) 137
Bsp120I GGGCCC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 2 cut(s) 236, 244
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 1 cut(s) 121
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 137
BspMAI CTGCAG 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 6, 53
BssMI GATC 2 cut(s) 236, 244
BssT1I CCWWGG 1 cut(s) 53
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 150
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 82
BstKTI GATC 2 cut(s) 239, 247
BstMBI GATC 2 cut(s) 236, 244
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 6
BstSFI CTRYAG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 67, 266
BsuI GTATCC 1 cut(s) 185
BsuRI GGCC 1 cut(s) 137
BtsCI GGATG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 146
CciI TCATGA 1 cut(s) 141
Cfr13I GGNCC 3 cut(s) 135, 136, 293
CviAII CATG 3 cut(s) 142, 221, 344
CviJI RGCY 6 cut(s) 32, 65, 137, 215, 279, 348
CviKI_1 RGCY 6 cut(s) 32, 65, 137, 215, 279, 348
DdeI CTNAG 1 cut(s) 129
DpnI GATC 2 cut(s) 238, 246
DpnII GATC 2 cut(s) 236, 244
DraI TTTAAA 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 53
EcoT22I ATGCAT 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 3 cut(s) 145, 224, 347
FatI CATG 3 cut(s) 141, 220, 343
Fnu4HI GCNGC 2 cut(s) 81, 280
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 2 cut(s) 81, 280
FspBI CTAG 1 cut(s) 228
GluI GCNGC 2 cut(s) 81, 280
HaeIII GGCC 1 cut(s) 137
Hin1II CATG 3 cut(s) 145, 224, 347
HphI GGTGA 1 cut(s) 47
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 1 cut(s) 142
HpyAV CCTTC 2 cut(s) 333, 366
HpyCH4III ACNGT 1 cut(s) 150
HpyCH4V TGCA 3 cut(s) 83, 119, 305
HpyF3I CTNAG 1 cut(s) 129
Hsp92II CATG 3 cut(s) 145, 224, 347
Kzo9I GATC 2 cut(s) 236, 244
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 6 cut(s) 18, 20, 69, 84, 219, 291
Lsp1109I GCAGC 2 cut(s) 67, 266
LweI GCATC 2 cut(s) 67, 104
MaeI CTAG 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 223
MalI GATC 2 cut(s) 238, 246
MboI GATC 2 cut(s) 236, 244
MboII GAAGA 2 cut(s) 243, 246
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 4 cut(s) 48, 103, 175, 325
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 3 cut(s) 31, 120, 149
Mph1103I ATGCAT 1 cut(s) 121
MseI TTAA 3 cut(s) 114, 152, 323
MspR9I CCNGG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 8
NdeII GATC 2 cut(s) 236, 244
NlaIII CATG 3 cut(s) 145, 224, 347
NlaIV GGNNCC 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 121
PagI TCATGA 1 cut(s) 141
PkrI GCNGC 2 cut(s) 82, 281
PsiI TTATAA 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 137
PspOMI GGGCCC 1 cut(s) 135
PspPI GGNCC 3 cut(s) 135, 136, 293
PstI CTGCAG 1 cut(s) 85
SaqAI TTAA 3 cut(s) 114, 152, 323
SatI GCNGC 2 cut(s) 81, 280
Sau3AI GATC 2 cut(s) 236, 244
Sau96I GGNCC 3 cut(s) 135, 136, 293
ScrFI CCNGG 1 cut(s) 8
SduI GDGCHC 1 cut(s) 139
SetI ASST 4 cut(s) 67, 112, 217, 281
SfaNI GCATC 2 cut(s) 67, 104
SfcI CTRYAG 1 cut(s) 81
SinI GGWCC 1 cut(s) 293
Sse9I AATT 4 cut(s) 48, 103, 175, 325
SspMI CTAG 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 6
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 150
TasI AATT 4 cut(s) 48, 103, 175, 325
Tru1I TTAA 3 cut(s) 114, 152, 323
Tru9I TTAA 3 cut(s) 114, 152, 323
TscAI CASTG 1 cut(s) 153
TseI GCWGC 2 cut(s) 80, 279
TspDTI ATGAA 3 cut(s) 318, 322, 348
TspRI CASTG 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 3 cut(s) 103, 175, 325
XspI CTAG 1 cut(s) 228
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.