MD15G1294100.v1.1

Plant transposase (Ptta/En/Spm family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0021148
Physical Location & Seq
Forward (+)
27441550 .. 27445812
4263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1294100.v1.1.491

Sequence Viewer

Length: 522 bp
ATGGCAGCGGGGAGTTCAAAATTCCCAGAGATCGATGTCTTTAGCGACGTTTATGTTCGACCCGGGGATGAGTTGACCGAGTCCCTTCATGCAGTTGGTTCTTCAGGAGTTCGCCTCCCAACTTCCTCCGATACTCCACTCGAGTCTGTGGATCCCCCAAAGGATGCAGGGTTTCAAATCCTTACGGAGACCTTGGATCAGACTCTCGGGCGAAGGCTAGGGACATATTGTAGGGGGATAGGGAATGCTAGGTGGCAGAAACTTAGAGCCCTTTCATCATCGCAATCAAACAATCAGGCGACAGCTTTGACAGCAGAGGTGGCCAGCCTTAGGACAAAACTCACCTCGTATAGGAGCCAAATGTCACAGCTTGTACAGGCCCTCAGTCAATCCGGCATTCGTCTCCCGAATTTGCATCCCCCATCGACCTCCGAGCCCTTCCAACCCAAGCATGGCCACACCTATGCCCCTTCGACCTCTGCCCCTATTGACAACCTTGAGACCTTCCAGCCGCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

174

Amino Acids

18.68

Weight (kDa)

6.41

Isoelectric Point (pI)

58.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021148)

Species Orthologous Gene IDs
malus_domestica MD08G1072500.v1.1 MD15G1294100.v1.1 MD16G1148800.v1.1
pyrus_communis pycom13g25430 pycom17g02620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 8, 512
AclWI GGATC 3 cut(s) 146, 159, 204
AcoI YGGCCR 2 cut(s) 321, 454
AcsI RAATTY 2 cut(s) 20, 409
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 3 cut(s) 330, 351, 452
AgsI TTSAA 2 cut(s) 18, 176
AluBI AGCT 2 cut(s) 305, 370
AluI AGCT 2 cut(s) 305, 370
Alw26I GTCTC 3 cut(s) 182, 407, 494
AlwI GGATC 3 cut(s) 146, 159, 204
Ama87I CYCGRG 3 cut(s) 62, 140, 206
AoxI GGCC 3 cut(s) 321, 378, 454
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 20, 409
AspS9I GGNCC 1 cut(s) 379
AsuC2I CCSGG 2 cut(s) 63, 64
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 3 cut(s) 62, 140, 206
AxyI CCTNAGG 1 cut(s) 329
BalI TGGCCA 2 cut(s) 323, 456
BamHI GGATCC 1 cut(s) 151
BanII GRGCYC 2 cut(s) 271, 438
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 430
BcnI CCSGG 2 cut(s) 63, 64
BcoDI GTCTC 3 cut(s) 182, 407, 494
BfaI CTAG 2 cut(s) 218, 249
BisI GCNGC 2 cut(s) 6, 512
BlsI GCNGC 2 cut(s) 7, 513
Bme1390I CCNGG 2 cut(s) 63, 64
BmeT110I CYCGRG 3 cut(s) 62, 140, 206
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 2 cut(s) 153, 356
BmrFI CCNGG 2 cut(s) 63, 64
BmsI GCATC 2 cut(s) 154, 424
BplI GAGNNNNNCTC 2 cut(s) 99, 131
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 2 cut(s) 63, 64
Bsa29I ATCGAT 1 cut(s) 33
BsaI GGTCTC 2 cut(s) 182, 494
BsaJI CCNNGG 3 cut(s) 62, 63, 192
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 351, 452
Bse21I CCTNAGG 1 cut(s) 329
BseCI ATCGAT 1 cut(s) 33
BseDI CCNNGG 3 cut(s) 62, 63, 192
BseGI GGATG 3 cut(s) 73, 169, 415
BseLI CCNNNNNNNGG 3 cut(s) 330, 351, 452
BseMII CTCAG 1 cut(s) 397
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 3 cut(s) 323, 380, 456
BshVI ATCGAT 1 cut(s) 33
BsiHKCI CYCGRG 3 cut(s) 62, 140, 206
BsiSI CCGG 2 cut(s) 63, 393
BslFI GGGAC 2 cut(s) 67, 235
BslI CCNNNNNNNGG 3 cut(s) 330, 351, 452
BsmAI GTCTC 3 cut(s) 182, 407, 494
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 67, 235
BsmI GAATGC 2 cut(s) 250, 396
BsnI GGCC 3 cut(s) 323, 380, 456
Bso31I GGTCTC 2 cut(s) 182, 494
BsoBI CYCGRG 3 cut(s) 62, 140, 206
Bsp1286I GDGCHC 2 cut(s) 271, 438
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 3 cut(s) 30, 151, 196
BspACI CCGC 2 cut(s) 8, 512
BspANI GGCC 3 cut(s) 323, 380, 456
BspCNI CTCAG 1 cut(s) 396
BspDI ATCGAT 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 153, 356
BspPI GGATC 3 cut(s) 146, 159, 204
BspTNI GGTCTC 2 cut(s) 182, 494
BsrGI TGTACA 1 cut(s) 373
BssECI CCNNGG 3 cut(s) 62, 63, 192
BssMI GATC 3 cut(s) 30, 151, 196
BssT1I CCWWGG 1 cut(s) 192
BstAUI TGTACA 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 3 cut(s) 263, 329, 383
BstENI CCTNNNNNAGG 1 cut(s) 349
BstF5I GGATG 3 cut(s) 73, 169, 415
BstKTI GATC 3 cut(s) 33, 154, 199
BstMAI GTCTC 3 cut(s) 182, 407, 494
BstMBI GATC 3 cut(s) 30, 151, 196
BstMWI GCNNNNNNNGC 2 cut(s) 311, 320
BstSCI CCNGG 2 cut(s) 61, 62
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 33
Bsu36I CCTNAGG 1 cut(s) 329
BsuRI GGCC 3 cut(s) 323, 380, 456
BsuTUI ATCGAT 1 cut(s) 33
BtgZI GCGATG 1 cut(s) 264
BtsCI GGATG 3 cut(s) 73, 169, 415
Cac8I GCNNGC 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 379
Cfr9I CCCGGG 1 cut(s) 62
ClaI ATCGAT 1 cut(s) 33
Csp6I GTAC 1 cut(s) 374
CviAII CATG 2 cut(s) 89, 452
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 3 cut(s) 263, 329, 383
DpnI GATC 3 cut(s) 32, 153, 198
DpnII GATC 3 cut(s) 30, 151, 196
EaeI YGGCCR 2 cut(s) 321, 454
Eco130I CCWWGG 1 cut(s) 192
Eco24I GRGCYC 2 cut(s) 271, 438
Eco31I GGTCTC 2 cut(s) 182, 494
Eco57I CTGAAG 1 cut(s) 87
Eco81I CCTNAGG 1 cut(s) 329
Eco88I CYCGRG 3 cut(s) 62, 140, 206
EcoNI CCTNNNNNAGG 1 cut(s) 349
EcoO109I RGGNCCY 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 192
EcoT38I GRGCYC 2 cut(s) 271, 438
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 2 cut(s) 92, 455
FaiI YATR 6 cut(s) 54, 90, 226, 351, 453, 465
FaqI GGGAC 2 cut(s) 67, 235
FatI CATG 2 cut(s) 88, 451
Fnu4HI GCNGC 2 cut(s) 6, 512
FokI GGATG 3 cut(s) 80, 176, 402
FriOI GRGCYC 2 cut(s) 271, 438
Fsp4HI GCNGC 2 cut(s) 6, 512
FspBI CTAG 2 cut(s) 218, 249
GluI GCNGC 2 cut(s) 6, 512
HaeIII GGCC 3 cut(s) 323, 380, 456
HapII CCGG 2 cut(s) 63, 393
Hin1II CATG 2 cut(s) 92, 455
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HinfI GANTC 3 cut(s) 80, 143, 202
HpaII CCGG 2 cut(s) 63, 393
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 3 cut(s) 130, 201, 433
Hpy188III TCNNGA 2 cut(s) 105, 406
Hpy8I GTNNAC 1 cut(s) 75
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 5 cut(s) 95, 207, 448, 480, 514
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 3 cut(s) 92, 167, 415
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 320
HpyF3I CTNAG 3 cut(s) 263, 329, 383
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 2 cut(s) 92, 455
Kzo9I GATC 3 cut(s) 30, 151, 196
LmnI GCTCC 1 cut(s) 354
LpnPI CCDG 8 cut(s) 39, 76, 90, 153, 281, 337, 362, 406
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 2 cut(s) 154, 424
MaeI CTAG 2 cut(s) 218, 249
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 363
MalI GATC 3 cut(s) 32, 153, 198
MboI GATC 3 cut(s) 30, 151, 196
MboII GAAGA 1 cut(s) 93
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 271, 438
MlsI TGGCCA 2 cut(s) 323, 456
MluCI AATT 2 cut(s) 20, 409
MluNI TGGCCA 2 cut(s) 323, 456
MlyI GAGTC 3 cut(s) 89, 152, 196
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 7 cut(s) 125, 136, 310, 355, 392, 439, 487
Mox20I TGGCCA 2 cut(s) 323, 456
MscI TGGCCA 2 cut(s) 323, 456
MslI CAYNNNNRTG 1 cut(s) 462
Msp20I TGGCCA 2 cut(s) 323, 456
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 63, 393
MspR9I CCNGG 2 cut(s) 63, 64
Mva1269I GAATGC 2 cut(s) 250, 396
MwoI GCNNNNNNNGC 2 cut(s) 311, 320
NciI CCSGG 2 cut(s) 63, 64
NdeII GATC 3 cut(s) 30, 151, 196
NlaIII CATG 2 cut(s) 92, 455
NlaIV GGNNCC 2 cut(s) 153, 356
NmuCI GTSAC 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 140
PctI GAATGC 2 cut(s) 250, 396
PflFI GACNNNGTC 1 cut(s) 79
PkrI GCNGC 2 cut(s) 7, 513
PleI GAGTC 3 cut(s) 88, 151, 196
PpsI GAGTC 3 cut(s) 88, 151, 196
PspN4I GGNNCC 2 cut(s) 153, 356
PspPI GGNCC 1 cut(s) 379
PspXI VCTCGAGB 1 cut(s) 140
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 1 cut(s) 79
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 462
SatI GCNGC 2 cut(s) 6, 512
Sau3AI GATC 3 cut(s) 30, 151, 196
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 3 cut(s) 89, 152, 196
ScrFI CCNGG 2 cut(s) 63, 64
SduI GDGCHC 2 cut(s) 271, 438
SfaNI GCATC 2 cut(s) 154, 424
Sfr274I CTCGAG 1 cut(s) 140
SlaI CTCGAG 1 cut(s) 140
SmaI CCCGGG 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 2 cut(s) 140, 497
SmoI CTYRAG 2 cut(s) 140, 497
Sse9I AATT 2 cut(s) 20, 409
SsiI CCGC 2 cut(s) 8, 512
SspMI CTAG 2 cut(s) 218, 249
StyD4I CCNGG 2 cut(s) 61, 62
StyI CCWWGG 1 cut(s) 192
TaiI ACGT 1 cut(s) 51
TaqI TCGA 5 cut(s) 33, 58, 141, 425, 473
TaqII GACCGA 1 cut(s) 92
TasI AATT 2 cut(s) 20, 409
TatI WGTACW 1 cut(s) 373
TauI GCSGC 1 cut(s) 514
TseFI GTSAC 1 cut(s) 363
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 363
TspDTI ATGAA 2 cut(s) 77, 264
TspGWI ACGGA 1 cut(s) 200
TspMI CCCGGG 1 cut(s) 62
Tth111I GACNNNGTC 1 cut(s) 79
XagI CCTNNNNNAGG 1 cut(s) 349
XapI RAATTY 2 cut(s) 20, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 449
XhoI CTCGAG 1 cut(s) 140
XmaI CCCGGG 1 cut(s) 62
XspI CTAG 2 cut(s) 218, 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.