pycom13g25430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0021148
Physical Location & Seq
Forward (+)
22233014 .. 22235032
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25430.5

Sequence Viewer

Length: 1590 bp
ATGATAATTTGGTCTTCAATTTCGTCCATCCCTTTTTCTCCAAACATAAGCTATCCATTCCAAGTCCGAGTTTGCTCCAAAATGCTCCAAAATGCATCTTTTTGCTTCCTTAGCCATATGAACCTAAAAACACACGAAAGAAGTACGAGAACAAGCCATTTAAGTCGCATGAATATGCTCCTATCAGTACTTATTGTCATGGGATGGGAAATGCCTGACGGCGGGAGAGTAGAGCCTCAAGTCCCTGGCCTAAGGAGTGAGCTGGCATTGTACAAGTCTCAAATGTCGATGCTTGTACAAGCCCTTTGTTTCTCCGGAATACATCTCCTCGGTTTTTTTGCGCCATTGCCCTCACAGCTCTTCCACACTAAGCAAGCACAACAATCAGGCCCGTCGACCTCCGACTCCGTCCCAAGCCAGCAGCAAGATCCTTTGAACGACATTCCTATAGATTTTTTCCGCTTTTTTTTCGTTGCGCAAACTTTGCGAGACGAACTAGTCACAAAGCGAACTTGTACCCTTTTATATCGTACGACGAAAACCTTCGTCCTTCTTTTTCAAGACGAGTTTGAAATTGTTTGTCGCGCAATGTCTTTTTTCACGACCTTTGCGCGACAAAATACGTGCGACAATTTTCTGGTCACCATAGTTTTTGTCCGACCTAATAATGATTTGCGCGACAAAATCTTCCTCATTGCGCAAACTCGATTAACTTGTGTTTTTGGTAAGCTGGAAATTCGTGTATTATTAAAGGTTGGATTTTCAATTACGGGCGCTATACATGTCAATTACGTGTCGAGAGATTTTGTCCATGAATTAAAGGCAGGATGTCCTGCAGAGAGAACACAACTGTATCATTCACAGTGTGACAAATATTACAACATCTTGCAGGAGAGAATGTACGTATATGTCAACAAAGAAATAGCACGAATTGCAGTCAGAAGCTTAAATATTCTTCCACATATGAAAGAATTGAATAGAACCTTGCTCTTAACAATTTTTTATTTGGCTTGTTCAGACAAAAGTTCTTCCAACCATCCTGCATCTTCTTTACTCGGTACAGAAAACACTGGAAGTTCATTCATTGTGATGAAGACCGGTTTCACTAGAAATTATACCCCAAGGCCCAAGGTACAAGTCCCCCCCACTCCTCCGCCGAAATCAGCAACACCACCAAAACAAACACCAACAATCATTGATATATTGGAGCCACTCTCTTTTCAGTTTAATTTTCAAGTTTGTAAAGCTCCACATCAACCATCAATTTCTCGTCAAAAAACATTAGTGCTGATAGAACTCACCTTTATATATACATGGAATTCAATGATCAACATACTCATCCTTGCATTTAGTGAGTTAGTTTCTATTTATTATAGTGATTTAAGCTATTTTCGTGTTTTGGAGCATTTTAGAGCAGTTTTGGGCTTGGAATGGATAGCGCATATATGGAGTAAGGGAAGAATTAATTCAAGACAAAGAAGATATGAAGCTCAGCAAGAAAGAAGGAATGTTAGCCAAGTTACCTTATTTTGTTGTAACCCTATTCTAATCCTACACCACCTAGGTTCCAGCTGCAAGAACGATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

530

Amino Acids

60.73

Weight (kDa)

9.37

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021148)

Species Orthologous Gene IDs
malus_domestica MD08G1072500.v1.1 MD15G1294100.v1.1 MD16G1148800.v1.1
pyrus_communis pycom13g25430 pycom17g02620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 497
Acc16I TGCGCA 2 cut(s) 477, 699
AccI GTMKAC 1 cut(s) 395
AccII CGCG 3 cut(s) 585, 613, 678
AccIII TCCGGA 1 cut(s) 314
AciI CCGC 3 cut(s) 222, 460, 1154
AclWI GGATC 2 cut(s) 422, 1577
AcsI RAATTY 2 cut(s) 735, 1318
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 9 cut(s) 145, 189, 272, 297, 517, 532, 902, 1060, 1134
AfiI CCNNNNNNNGG 1 cut(s) 221
AflIII ACRYGT 2 cut(s) 781, 792
AgeI ACCGGT 1 cut(s) 1097
AgsI TTSAA 9 cut(s) 18, 436, 560, 572, 765, 976, 1235, 1323, 1470
AhlI ACTAGT 1 cut(s) 496
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 9 cut(s) 51, 262, 358, 730, 945, 1247, 1386, 1490, 1572
AluI AGCT 9 cut(s) 51, 262, 358, 730, 945, 1247, 1386, 1490, 1572
Alw26I GTCTC 2 cut(s) 282, 483
AlwI GGATC 2 cut(s) 422, 1577
Aor13HI TCCGGA 1 cut(s) 314
AoxI GGCC 3 cut(s) 247, 388, 1124
ApeKI GCWGC 2 cut(s) 421, 1572
ApoI RAATTY 2 cut(s) 735, 1318
AseI ATTAAT 1 cut(s) 1464
AsiGI ACCGGT 1 cut(s) 1097
Asp700I GAANNNNTTC 2 cut(s) 542, 1465
AspA2I CCTAGG 1 cut(s) 1561
AspLEI GCGC 8 cut(s) 343, 478, 587, 613, 678, 700, 776, 1441
AspS9I GGNCC 2 cut(s) 389, 1125
AsuHPI GGTGA 2 cut(s) 634, 1291
AvrII CCTAGG 1 cut(s) 1561
AxyI CCTNAGG 1 cut(s) 251
BaeI ACNNNNGTAYC 2 cut(s) 836, 869
BbsI GAAGAC 2 cut(s) 6, 1100
BbvI GCAGC 2 cut(s) 433, 1559
BccI CCATC 4 cut(s) 35, 198, 1044, 1267
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 246
BclI TGATCA 1 cut(s) 1326
BcoDI GTCTC 2 cut(s) 282, 483
BcuI ACTAGT 1 cut(s) 496
BfaI CTAG 3 cut(s) 497, 1107, 1562
BfmI CTRYAG 2 cut(s) 447, 834
BfoI RGCGCY 1 cut(s) 777
BisI GCNGC 2 cut(s) 422, 1573
BlnI CCTAGG 1 cut(s) 1561
BlpI GCTNAGC 1 cut(s) 1491
BlsI GCNGC 2 cut(s) 423, 1574
BmcAI AGTACT 1 cut(s) 189
Bme1390I CCNGG 1 cut(s) 246
BmgT120I GGNCC 2 cut(s) 389, 1125
BmiI GGNNCC 2 cut(s) 1209, 1567
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 3 cut(s) 104, 279, 1052
BpiI GAAGAC 2 cut(s) 6, 1100
BplI GAGNNNNNCTC 2 cut(s) 1199, 1231
Bpu10I CCTNAGC 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 1491
BpuEI CTTGAG 1 cut(s) 222
BsaAI YACGTR 3 cut(s) 624, 793, 904
BsaJI CCNNGG 5 cut(s) 244, 328, 1121, 1128, 1561
BsaWI WCCGGW 2 cut(s) 314, 1097
BsaXI ACNNNNNCTCC 2 cut(s) 884, 914
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse118I RCCGGY 1 cut(s) 1097
Bse1I ACTGG 1 cut(s) 1075
Bse21I CCTNAGG 1 cut(s) 251
Bse3DI GCAATG 3 cut(s) 344, 594, 693
BseAI TCCGGA 1 cut(s) 314
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 5 cut(s) 244, 328, 1121, 1128, 1561
BseGI GGATG 5 cut(s) 27, 209, 833, 1036, 1338
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMI GCAATG 3 cut(s) 344, 594, 693
BseMII CTCAG 1 cut(s) 1505
BseNI ACTGG 1 cut(s) 1075
BseRI GAGGAG 2 cut(s) 317, 1140
BseXI GCAGC 2 cut(s) 433, 1559
Bsh1236I CGCG 3 cut(s) 585, 613, 678
BshFI GGCC 3 cut(s) 249, 390, 1126
BshTI ACCGGT 1 cut(s) 1097
BsiSI CCGG 2 cut(s) 315, 1098
BsiWI CGTACG 1 cut(s) 530
BslFI GGGAC 3 cut(s) 227, 395, 1124
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 2 cut(s) 282, 483
BsmBI CGTCTC 1 cut(s) 483
BsmFI GGGAC 3 cut(s) 227, 395, 1124
BsnI GGCC 3 cut(s) 249, 390, 1126
Bsp13I TCCGGA 1 cut(s) 314
Bsp1407I TGTACA 2 cut(s) 270, 295
Bsp143I GATC 3 cut(s) 427, 1326, 1582
Bsp1720I GCTNAGC 1 cut(s) 1491
BspACI CCGC 3 cut(s) 222, 460, 1154
BspANI GGCC 3 cut(s) 249, 390, 1126
BspCNI CTCAG 1 cut(s) 1504
BspEI TCCGGA 1 cut(s) 314
BspFNI CGCG 3 cut(s) 585, 613, 678
BspLI GGNNCC 2 cut(s) 1209, 1567
BspMAI CTGCAG 1 cut(s) 838
BspPI GGATC 2 cut(s) 422, 1577
BspQI GCTCTTC 1 cut(s) 365
BsrDI GCAATG 3 cut(s) 344, 594, 693
BsrFI RCCGGY 1 cut(s) 1097
BsrGI TGTACA 2 cut(s) 270, 295
BsrI ACTGG 1 cut(s) 1075
BssAI RCCGGY 1 cut(s) 1097
BssECI CCNNGG 5 cut(s) 244, 328, 1121, 1128, 1561
BssMI GATC 3 cut(s) 427, 1326, 1582
BssT1I CCWWGG 3 cut(s) 1121, 1128, 1561
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 852, 864
Bst6I CTCTTC 1 cut(s) 365
BstAPI GCANNNNNTGC 2 cut(s) 484, 932
BstAUI TGTACA 2 cut(s) 270, 295
BstBAI YACGTR 3 cut(s) 624, 793, 904
BstC8I GCNNGC 3 cut(s) 264, 375, 419
BstDEI CTNAG 4 cut(s) 110, 251, 369, 1491
BstEII GGTNACC 1 cut(s) 640
BstF5I GGATG 5 cut(s) 27, 209, 833, 1036, 1338
BstFNI CGCG 3 cut(s) 585, 613, 678
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 8 cut(s) 343, 478, 587, 613, 678, 700, 776, 1441
BstKTI GATC 3 cut(s) 430, 1329, 1585
BstMAI GTCTC 2 cut(s) 282, 483
BstMBI GATC 3 cut(s) 427, 1326, 1582
BstMWI GCNNNNNNNGC 4 cut(s) 111, 355, 484, 932
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 1 cut(s) 785
BstPI GGTNACC 1 cut(s) 640
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 2 cut(s) 447, 834
BstSNI TACGTA 1 cut(s) 904
BstUI CGCG 3 cut(s) 585, 613, 678
BstV1I GCAGC 2 cut(s) 433, 1559
BstV2I GAAGAC 2 cut(s) 6, 1100
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
Bsu36I CCTNAGG 1 cut(s) 251
BsuRI GGCC 3 cut(s) 249, 390, 1126
BtsCI GGATG 5 cut(s) 27, 209, 833, 1036, 1338
BtsIMutI CAGTG 2 cut(s) 869, 1068
Cac8I GCNNGC 3 cut(s) 264, 375, 419
CfoI GCGC 8 cut(s) 343, 478, 587, 613, 678, 700, 776, 1441
Cfr10I RCCGGY 1 cut(s) 1097
Cfr13I GGNCC 2 cut(s) 389, 1125
Csp6I GTAC 9 cut(s) 144, 188, 271, 296, 516, 531, 901, 1059, 1133
CspAI ACCGGT 1 cut(s) 1097
CviAII CATG 5 cut(s) 169, 199, 782, 812, 1314
CviQI GTAC 9 cut(s) 144, 188, 271, 296, 516, 531, 901, 1059, 1133
DdeI CTNAG 4 cut(s) 110, 251, 369, 1491
DpnI GATC 3 cut(s) 429, 1328, 1584
DpnII GATC 3 cut(s) 427, 1326, 1582
DraIII CACNNNGTG 1 cut(s) 866
DrdI GACNNNNNNGTC 1 cut(s) 497
DseDI GACNNNNNNGTC 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
EciI GGCGGA 1 cut(s) 1143
Eco105I TACGTA 1 cut(s) 904
Eco130I CCWWGG 3 cut(s) 1121, 1128, 1561
Eco81I CCTNAGG 1 cut(s) 251
Eco91I GGTNACC 1 cut(s) 640
EcoO65I GGTNACC 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 1318
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 3 cut(s) 1121, 1128, 1561
EcoT22I ATGCAT 1 cut(s) 97
ErhI CCWWGG 3 cut(s) 1121, 1128, 1561
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 5 cut(s) 172, 202, 785, 815, 1317
FaqI GGGAC 3 cut(s) 227, 395, 1124
FatI CATG 5 cut(s) 168, 198, 781, 811, 1313
FauI CCCGC 1 cut(s) 215
FauNDI CATATG 2 cut(s) 117, 963
FbaI TGATCA 1 cut(s) 1326
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 2 cut(s) 422, 1573
FokI GGATG 5 cut(s) 14, 216, 840, 1023, 1325
Fsp4HI GCNGC 2 cut(s) 422, 1573
FspBI CTAG 3 cut(s) 497, 1107, 1562
FspI TGCGCA 2 cut(s) 477, 699
GlaI GCGC 8 cut(s) 342, 477, 586, 612, 677, 699, 775, 1440
GluI GCNGC 2 cut(s) 422, 1573
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 3 cut(s) 249, 390, 1126
HapII CCGG 2 cut(s) 315, 1098
HhaI GCGC 8 cut(s) 343, 478, 587, 613, 678, 700, 776, 1441
Hin1II CATG 5 cut(s) 172, 202, 785, 815, 1317
Hin6I GCGC 8 cut(s) 341, 476, 585, 611, 676, 698, 774, 1439
HinP1I GCGC 8 cut(s) 341, 476, 585, 611, 676, 698, 774, 1439
HincII GTYRAC 2 cut(s) 396, 913
HindII GTYRAC 2 cut(s) 396, 913
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 1 cut(s) 404
HpaII CCGG 2 cut(s) 315, 1098
HphI GGTGA 2 cut(s) 634, 1291
Hpy166II GTNNAC 2 cut(s) 396, 913
Hpy188I TCNGA 5 cut(s) 68, 403, 659, 941, 1018
Hpy188III TCNNGA 5 cut(s) 315, 560, 601, 798, 1470
Hpy8I GTNNAC 2 cut(s) 396, 913
Hpy99I CGWCG 2 cut(s) 397, 538
HpyAV CCTTC 3 cut(s) 553, 560, 1497
HpyCH4III ACNGT 2 cut(s) 852, 864
HpyCH4IV ACGT 3 cut(s) 623, 792, 903
HpyCH4V TGCA 7 cut(s) 95, 836, 889, 935, 1043, 1346, 1575
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 355, 484, 932
HpyF3I CTNAG 4 cut(s) 110, 251, 369, 1491
HpySE526I ACGT 3 cut(s) 623, 792, 903
Hsp92II CATG 5 cut(s) 172, 202, 785, 815, 1317
HspAI GCGC 8 cut(s) 341, 476, 585, 611, 676, 698, 774, 1439
Kpn2I TCCGGA 1 cut(s) 314
Ksp22I TGATCA 1 cut(s) 1326
Kzo9I GATC 3 cut(s) 427, 1326, 1582
LguI GCTCTTC 1 cut(s) 365
LmnI GCTCC 6 cut(s) 80, 90, 183, 1207, 1252, 1402
Lsp1109I GCAGC 2 cut(s) 433, 1559
LweI GCATC 3 cut(s) 104, 279, 1052
MaeI CTAG 3 cut(s) 497, 1107, 1562
MaeII ACGT 3 cut(s) 623, 792, 903
MaeIII GTNAC 5 cut(s) 499, 640, 866, 1519, 1535
MalI GATC 3 cut(s) 429, 1328, 1584
MboI GATC 3 cut(s) 427, 1326, 1582
MboII GAAGA 9 cut(s) 6, 352, 679, 947, 1020, 1038, 1105, 1470, 1491
MflI RGATCY 1 cut(s) 427
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 4 cut(s) 426, 682, 736, 1056
MnlI CCTC 6 cut(s) 246, 338, 361, 409, 701, 1161
Mph1103I ATGCAT 1 cut(s) 97
MroI TCCGGA 1 cut(s) 314
MroXI GAANNNNTTC 2 cut(s) 542, 1465
MseI TTAA 9 cut(s) 161, 710, 749, 818, 947, 992, 1227, 1382, 1464
MslI CAYNNNNRTG 2 cut(s) 173, 1088
MspA1I CMGCKG 1 cut(s) 1572
MspI CCGG 2 cut(s) 315, 1098
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 3 cut(s) 585, 613, 678
MwoI GCNNNNNNNGC 4 cut(s) 111, 355, 484, 932
NdeI CATATG 2 cut(s) 117, 963
NdeII GATC 3 cut(s) 427, 1326, 1582
NlaIII CATG 5 cut(s) 172, 202, 785, 815, 1317
NlaIV GGNNCC 2 cut(s) 1209, 1567
NmuCI GTSAC 3 cut(s) 499, 640, 866
NsbI TGCGCA 2 cut(s) 477, 699
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 1 cut(s) 785
PciI ACATGT 1 cut(s) 781
PciSI GCTCTTC 1 cut(s) 365
PdmI GAANNNNTTC 2 cut(s) 542, 1465
Pfl23II CGTACG 1 cut(s) 530
PflFI GACNNNGTC 1 cut(s) 407
PinAI ACCGGT 1 cut(s) 1097
PkrI GCNGC 2 cut(s) 423, 1574
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
Ppu21I YACGTR 3 cut(s) 624, 793, 904
PscI ACATGT 1 cut(s) 781
PshBI ATTAAT 1 cut(s) 1464
Psp6I CCWGG 1 cut(s) 244
PspEI GGTNACC 1 cut(s) 640
PspGI CCWGG 1 cut(s) 244
PspLI CGTACG 1 cut(s) 530
PspN4I GGNNCC 2 cut(s) 1209, 1567
PspPI GGNCC 2 cut(s) 389, 1125
PstI CTGCAG 1 cut(s) 838
PsuI RGATCY 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 1572
RsaI GTAC 9 cut(s) 145, 189, 272, 297, 517, 532, 902, 1060, 1134
RsaNI GTAC 9 cut(s) 144, 188, 271, 296, 516, 531, 901, 1059, 1133
RseI CAYNNNNRTG 2 cut(s) 173, 1088
SalI GTCGAC 1 cut(s) 394
SapI GCTCTTC 1 cut(s) 365
SaqAI TTAA 9 cut(s) 161, 710, 749, 818, 947, 992, 1227, 1382, 1464
SatI GCNGC 2 cut(s) 422, 1573
Sau3AI GATC 3 cut(s) 427, 1326, 1582
Sau96I GGNCC 2 cut(s) 389, 1125
ScaI AGTACT 1 cut(s) 189
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 1 cut(s) 246
SfaNI GCATC 3 cut(s) 104, 279, 1052
SfcI CTRYAG 2 cut(s) 447, 834
SmiMI CAYNNNNRTG 2 cut(s) 173, 1088
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SnaBI TACGTA 1 cut(s) 904
SpeI ACTAGT 1 cut(s) 496
SsiI CCGC 3 cut(s) 222, 460, 1154
SspI AATATT 2 cut(s) 875, 952
SspMI CTAG 3 cut(s) 497, 1107, 1562
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 3 cut(s) 1121, 1128, 1561
TaaI ACNGT 2 cut(s) 852, 864
TaiI ACGT 3 cut(s) 626, 795, 906
TaqI TCGA 4 cut(s) 287, 395, 706, 797
TatI WGTACW 3 cut(s) 187, 270, 295
Tru1I TTAA 9 cut(s) 161, 710, 749, 818, 947, 992, 1227, 1382, 1464
Tru9I TTAA 9 cut(s) 161, 710, 749, 818, 947, 992, 1227, 1382, 1464
TscAI CASTG 2 cut(s) 869, 1075
TseFI GTSAC 3 cut(s) 499, 640, 866
TseI GCWGC 2 cut(s) 421, 1572
Tsp45I GTSAC 3 cut(s) 499, 640, 866
TspDTI ATGAA 8 cut(s) 134, 185, 828, 980, 1068, 1072, 1106, 1500
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 2 cut(s) 869, 1075
Tth111I GACNNNGTC 1 cut(s) 407
VspI ATTAAT 1 cut(s) 1464
XapI RAATTY 2 cut(s) 735, 1318
XceI RCATGY 1 cut(s) 785
XmaJI CCTAGG 1 cut(s) 1561
XmiI GTMKAC 1 cut(s) 395
XmnI GAANNNNTTC 2 cut(s) 542, 1465
XspI CTAG 3 cut(s) 497, 1107, 1562
ZrmI AGTACT 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.