MD15G1297300.v1.1

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0019275
Physical Location & Seq
Forward (+)
28025303 .. 28025653
351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1297300.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGTTGTGTCAGTCACAGCATCAACTACAAGTGGCAAGCCACTTCTAGAGGAAGTCCTTGAAAAAACAATTGAGGCATGCGATCGAGCCCTTGCTCTTGATTCAGCAAAGAAGATGGTGCTTGATTTCGTTGAAAGTAGAATGGGTTTTATTGCACCGAATCTTTCTGCTATCATTGGTAGTGCTGTTGCGGCAAAACTTATGGGGACAGCTGGGGGCCTCGCATCCTTAGCTAAGATGCCACCTTGTAATGTTCAGCTTCTTGGTGCTAAGAGAAACAACCTTGCCGGATTTTCCACTGCAACATCACACTCCCGTGTTGGTTATGTTGAGCAGACAGAAAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

117

Amino Acids

12.09

Weight (kDa)

7.78

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 1 - 113 1.1e-32 snoRNA binding domain, fibrillarin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019275)

Species Orthologous Gene IDs
malus_domestica MD15G1297300.v1.1
pyrus_communis pycom15g26040
rosa_laevigata RLG00000022097
rosa_multiflora Rmu_sc0024549.1_g000005 Rmu_sc0025855.1_g000002
rosa_rugosa Rorug02G0557900
rosa_samantha Rh2CG613500 Rh2DG661400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 191
AcsI RAATTY 1 cut(s) 343
AgsI TTSAA 2 cut(s) 62, 134
AleI CACNNNNGTG 1 cut(s) 315
AluBI AGCT 3 cut(s) 212, 233, 259
AluI AGCT 3 cut(s) 212, 233, 259
AoxI GGCC 1 cut(s) 217
ApoI RAATTY 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 91
BccI CCATC 1 cut(s) 109
BfaI CTAG 1 cut(s) 47
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 218
BmsI GCATC 3 cut(s) 29, 228, 233
Bpu10I CCTNAGC 1 cut(s) 229
BseGI GGATG 1 cut(s) 224
BseYI CCCAGC 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 85
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 91
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 1 cut(s) 191
BspANI GGCC 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 218
BssMI GATC 1 cut(s) 82
BstC8I GCNNGC 2 cut(s) 38, 79
BstDEI CTNAG 3 cut(s) 229, 234, 270
BstF5I GGATG 1 cut(s) 224
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 2 cut(s) 191, 230
BstNSI RCATGY 1 cut(s) 81
BsuRI GGCC 1 cut(s) 219
BtsCI GGATG 1 cut(s) 224
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 2 cut(s) 38, 79
Cfr13I GGNCC 1 cut(s) 217
CviAII CATG 1 cut(s) 78
CviJI RGCY 6 cut(s) 40, 89, 212, 219, 233, 259
CviKI_1 RGCY 6 cut(s) 40, 89, 212, 219, 233, 259
DdeI CTNAG 3 cut(s) 229, 234, 270
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 91
FaeI CATG 1 cut(s) 81
FaiI YATR 3 cut(s) 79, 203, 327
FaqI GGGAC 1 cut(s) 220
FatI CATG 1 cut(s) 77
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 1 cut(s) 211
FriOI GRGCYC 1 cut(s) 91
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 1 cut(s) 47
GluI GCNGC 1 cut(s) 192
GsaI CCCAGC 1 cut(s) 216
HaeIII GGCC 1 cut(s) 219
HapII CCGG 1 cut(s) 288
Hin1II CATG 1 cut(s) 81
HinfI GANTC 2 cut(s) 101, 160
HpaII CCGG 1 cut(s) 288
Hpy188III TCNNGA 2 cut(s) 47, 98
HpyCH4V TGCA 2 cut(s) 155, 302
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 230
HpyF3I CTNAG 3 cut(s) 229, 234, 270
Hsp92II CATG 1 cut(s) 81
Kzo9I GATC 1 cut(s) 82
LpnPI CCDG 2 cut(s) 198, 301
LweI GCATC 3 cut(s) 29, 228, 233
MaeI CTAG 1 cut(s) 47
MaeIII GTNAC 1 cut(s) 13
MalI GATC 1 cut(s) 84
MboI GATC 1 cut(s) 82
MboII GAAGA 1 cut(s) 124
MfeI CAATTG 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 2 cut(s) 69, 343
MnlI CCTC 3 cut(s) 43, 67, 230
MseI TTAA 1 cut(s) 349
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 1 cut(s) 288
MunI CAATTG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 191, 230
NdeII GATC 1 cut(s) 82
NlaIII CATG 1 cut(s) 81
NlaIV GGNNCC 1 cut(s) 218
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 1 cut(s) 81
OliI CACNNNNGTG 1 cut(s) 315
PaeI GCATGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 101, 160
PkrI GCNGC 1 cut(s) 193
Ple19I CGATCG 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 212
PspN4I GGNNCC 1 cut(s) 218
PspPI GGNCC 1 cut(s) 217
PvuI CGATCG 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 212
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 1 cut(s) 349
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 1 cut(s) 82
Sau96I GGNCC 1 cut(s) 217
SduI GDGCHC 1 cut(s) 91
SetI ASST 5 cut(s) 214, 235, 247, 261, 285
SfaNI GCATC 3 cut(s) 29, 228, 233
SmiMI CAYNNNNRTG 1 cut(s) 315
SphI GCATGC 1 cut(s) 81
Sse9I AATT 2 cut(s) 69, 343
SsiI CCGC 1 cut(s) 191
SspMI CTAG 1 cut(s) 47
TaqI TCGA 1 cut(s) 85
TasI AATT 2 cut(s) 69, 343
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 101, 160
Tru1I TTAA 1 cut(s) 349
Tru9I TTAA 1 cut(s) 349
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 13
TspRI CASTG 1 cut(s) 304
XapI RAATTY 1 cut(s) 343
XbaI TCTAGA 1 cut(s) 46
XceI RCATGY 1 cut(s) 81
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.