RLG00000022097

U4 U6 small nuclear ribonucleoprotein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019275
Physical Location & Seq
Reverse (-)
80912523 .. 80913184
662 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022097

Sequence Viewer

Length: 348 bp
ATGGATGTAACCCTTGTTGATCTGGAGGGGCTTTTATCTTCAACAGCTATTATGGATGTGTCAGTTACAGCTTCAACTACAAGTGGAAAGCCACTTCCGGAGGACATCCTAGAGAAAATAGTTGATGCATGTGATCAAGCCCTTGCACTCGAACCAACGAAGAAAAAAGTTTTTGATTTTGTAGAAGGGAGAATGGGTTCTATTGCACCAAATCTTTGTGCTATTGTTGGGAGTGTGGTTGCGGCGAACCTATGGGGACAGCTGGGGATTGAGCTCTCGAATCCTCAGGCCCATGGACAGCAACTTGGCAGTGGAACTCAAAAGCACGCATACTTCTCTGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Nop Prp31_C

Protein Analysis

116

Amino Acids

12.09

Weight (kDa)

4.52

Isoelectric Point (pI)

24.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 12 - 89 9.8e-18 snoRNA binding domain, fibrillarin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019275)

Species Orthologous Gene IDs
malus_domestica MD15G1297300.v1.1
pyrus_communis pycom15g26040
rosa_laevigata RLG00000022097
rosa_multiflora Rmu_sc0024549.1_g000005 Rmu_sc0025855.1_g000002
rosa_rugosa Rorug02G0557900
rosa_samantha Rh2CG613500 Rh2DG661400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 1 cut(s) 242
AgsI TTSAA 2 cut(s) 42, 75
AloI GAACNNNNNNTCC 2 cut(s) 181, 213
AluBI AGCT 4 cut(s) 47, 71, 262, 274
AluI AGCT 4 cut(s) 47, 71, 262, 274
Alw21I GWGCWC 1 cut(s) 276
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 289
AxyI CCTNAGG 1 cut(s) 285
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 276
BclI TGATCA 1 cut(s) 133
BfaI CTAG 1 cut(s) 110
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 289
BmsI GCATC 1 cut(s) 115
BpmI CTGGAG 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 292
BsaWI WCCGGW 1 cut(s) 97
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bse21I CCTNAGG 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 292
BseGI GGATG 3 cut(s) 10, 61, 105
BseMII CTCAG 1 cut(s) 299
BseYI CCCAGC 1 cut(s) 262
BshFI GGCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 1 cut(s) 98
BslFI GGGAC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 270
BsnI GGCC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 276
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 2 cut(s) 19, 133
Bsp19I CCATGG 1 cut(s) 292
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 298
BspEI TCCGGA 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 292
BssMI GATC 2 cut(s) 19, 133
BssT1I CCWWGG 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 1 cut(s) 292
BstF5I GGATG 3 cut(s) 10, 61, 105
BstKTI GATC 2 cut(s) 22, 136
BstMBI GATC 2 cut(s) 19, 133
BstNSI RCATGY 1 cut(s) 132
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 1 cut(s) 292
BtsCI GGATG 3 cut(s) 10, 61, 105
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 289
CviAII CATG 3 cut(s) 129, 293, 345
CviJI RGCY 8 cut(s) 31, 47, 71, 91, 140, 262, 274, 290
CviKI_1 RGCY 8 cut(s) 31, 47, 71, 91, 140, 262, 274, 290
DdeI CTNAG 1 cut(s) 285
DpnI GATC 2 cut(s) 21, 135
DpnII GATC 2 cut(s) 19, 133
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 274
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 292
FaeI CATG 3 cut(s) 132, 296, 348
FaiI YATR 6 cut(s) 53, 130, 253, 294, 331, 346
FaqI GGGAC 1 cut(s) 270
FatI CATG 3 cut(s) 128, 292, 344
FbaI TGATCA 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 3 cut(s) 17, 68, 92
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 1 cut(s) 110
GluI GCNGC 1 cut(s) 243
GsaI CCCAGC 1 cut(s) 266
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 1 cut(s) 290
HapII CCGG 1 cut(s) 98
Hin1II CATG 3 cut(s) 132, 296, 348
HinfI GANTC 1 cut(s) 280
HpaII CCGG 1 cut(s) 98
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 3 cut(s) 23, 98, 277
HpyAV CCTTC 1 cut(s) 179
HpyCH4V TGCA 3 cut(s) 128, 146, 206
HpyF3I CTNAG 1 cut(s) 285
Hsp92II CATG 3 cut(s) 132, 296, 348
Kpn2I TCCGGA 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 2 cut(s) 19, 133
LpnPI CCDG 4 cut(s) 8, 111, 248, 272
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 110
MaeIII GTNAC 2 cut(s) 7, 64
MalI GATC 2 cut(s) 21, 135
MboI GATC 2 cut(s) 19, 133
MboII GAAGA 2 cut(s) 30, 172
MhlI GDGCHC 1 cut(s) 276
MnlI CCTC 3 cut(s) 19, 94, 294
Mph1103I ATGCAT 1 cut(s) 130
MroI TCCGGA 1 cut(s) 97
MroXI GAANNNNTTC 1 cut(s) 196
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 1 cut(s) 98
NcoI CCATGG 1 cut(s) 292
NdeII GATC 2 cut(s) 19, 133
NlaIII CATG 3 cut(s) 132, 296, 348
NsiI ATGCAT 1 cut(s) 130
NspI RCATGY 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 1 cut(s) 244
Psp124BI GAGCTC 1 cut(s) 276
PspFI CCCAGC 1 cut(s) 262
PspPI GGNCC 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 262
SacI GAGCTC 1 cut(s) 276
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 19, 133
Sau96I GGNCC 1 cut(s) 289
SduI GDGCHC 1 cut(s) 276
SetI ASST 5 cut(s) 49, 73, 252, 264, 276
SfaNI GCATC 1 cut(s) 115
SsiI CCGC 1 cut(s) 242
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 1 cut(s) 276
StyI CCWWGG 1 cut(s) 292
TaqI TCGA 2 cut(s) 150, 278
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 280
TscAI CASTG 1 cut(s) 316
TspRI CASTG 1 cut(s) 316
XceI RCATGY 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 196
XspI CTAG 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.