Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005351

Physical Location & Seq

Genomic Coordinates Forward (+)

31727227 .. 31735301

Gene Structure 8075 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1313700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2955 bp

ATGCCATGGCTAACGTGCACGAAGCTGACGCTTTTCACCATAGCTGGAGTTGCGATTCAAATGGTTGGCCTCTCCATCTTCGTCTTCGGCTTCTTCCCCGTCAAACCAACCCTTCCCGGACACAGTGGTTCGGAGAGTTTTCGAGCACCGCAGTGCGATTTGGTACGAAATGAGAGTGAGAGTGATCTGCATCCCCATCAGCTCAGGTCACTATATAAGGAACTATCAGGAATGCCGCCTGCATTTGACCGACTAATACTAATGGTTATTGATGGTCTTCCAGCAGAACTCGTGCTCGGCAAGGATGGTAAGCCTCCAAGCAAGGCGTTAATGGAAGCTATGCCATATACTCGGTCACTGCTTGCAAATGGAATGGCAATTGGGTTCCATGCAAAGGCTGCACCTCCAACTGTTACAATGCCTCGTTTGAAGGCTATGGTTTCTGGAGCAATAGGGGGATTCCTGGATGTGGCTATGAATTTTAACACACAAGCTATGTTAGATGACAATCTTCTCGGCCAGTTTTTCAAGATTGGTTGGAAAATGGTGATGCTTGGTGATGAAACATGGCTTAAGTTGTTTCCCGGATTATTTTTGAGGCATGATGGAGTCAGCAGTTTCTTTGTTAAAGATACTGTACACGTGGATCAAAATGTTTCTCGACATCTGGGTTATGAACTTAGCAGAGATGACTGGGATTTTCTGATTCTCCACTATTTGGGCTTAGATCATGTTGGACATATTGGTGGGCGCAACAGTGCCTTGATGGCCCCAAAACTTCGAGAGATGGATGAGGTGGTGAAGATGATTCATATGAATAGTATACTAAATCAAAAGAATGATGAAGGACAGACACTCTTGGTTGTGGTAAGTGACCATGGCATGACTGAGAGTGGTAATCACGGAGGGTCCTCCTATGAAGAAACTGACTCCTTAGCTCTTTTTATTGGCGTGAAAAATAACATCTCTGATCACTTCTTGTCCACCCATAACATTATTCACCAGGTTGACATTGCACCAACATTAGCTCTTCTATTTGGTGTTCCAATTCCAAAAAACAACGTTGGTGTTCTGATTGCTGAAATTTTTGGGTATTTGTCTGAAGACCAACAGATGAAGGCACTGGAGTTGAATTCCTGGCAATTACTTAGATTATTGCAAGCACAAATACCTGGTTTATCATGTCGAAATTACCCACGTGATGGCTTAAGGGATGATCAAGACTTCAGAATAGCTAAGTGCTCTGGGAGCATGGAAGCGATGTTTTGCTGCCTATATACAAATGCTGCACTTCTCCAAAAGTCCTGGATGTCTAAGGCAATCTCCAGGTCTAACAGTAGGGAAGACTACACTAGCACGGTTGTAGCATACTATGAGTTTCTCAGAACTGCAAGTGAGTGGTTGTCACACAGAGCAACTGATAAACCTGTCAGACTACTTACTGTCGGAATTGCTGCAATGCTCCTATCTTGTCTGATATTATTAAGCCTTCTATTTCATACATGCAAAGAAGTCTACCTTAGAGAGCAGTCCCTTTTTGACTTAGAGAGCATCAAGCAGACCTGGTATTTAGACGAGACTTTTGTATTAGGCGTAGTATTGATTCTCATTGTAAGTATGGCATCAAGTTCTATGGTAGAAGAAGAGCAATATATTTGGCATTTTGTGGCTTCCACATTGAATTTGCTATTATTGCGTAAAACAATAAAATGTCCTACAGGGGCAGGAGCACAAGATCTCCATAGTTCATCCAAGGGAAAAAACAAAATAAGTTTTTGTCGATTGAGTTCCATCTCTGTGGTTCTTATTTCTGGAAGGATATTGAGAGGCTGGCACCAAGGAGGTGTGAATTGGACTAATCTTCCTGACATTTCAAAGTGGCTTGAGCAGGCTGGGACTGATAATGTAAAATCAATTCAGCTAGTCTCATGCCTTCTAGTAATGAGCTTAAGCTTATGTGTTCTTTTTCTATTTGGATTCAACATGAAATTTGTTCTGGTGATTGGATTCAGCTTTCTTATGTCTGGATTGCTGGTTTTGCAACATTTTGTTAAACATCAAGATGGTATGTTTGCGTCATCGAGTTGCAGTACAACAACATTGGTACAAATAATTTATGCAATTATGGGTATTTCCACATTTGGGACTGTTGTAGCCTTACCATGGTTCGTACGTTTCTCAACATCTGAGATGGGCTTGAGTCATGATTTGTACATGTCAACTTCTGTTCCCAATGAAGTTCAAAATAACACGCTGCTGGTGAAATTAAGAGAATCTCTGTATCTAGTTGGGTGGGAATACATCTCTTTCTGGTGTCTTCTGCAACTGCTGCTTCAACAAACAATTAACTCAATGACTATAGTGTTGCTACTTGTGCAAATTTTGGCCAGCATGTTGTATTTTTCTTATAGTGGCCTGCATCAGAAGCAATGGGTTGAGATAGCTGCATTTTACTTTTTGGGAATGGCCGGCCATTTTGCTCTGGGTAACAGCAATTCACTAGCTACTATTGATGTTGCCGGAGCTTTTATGGGAATCTCAAATCACTCGACAGTACTTTCTGGAATTTTAATGTTCATTATCACCTATGCATCCCCAATGTTATTTATCCTTAGCATGGTGATGTATGTCTCTGTGAAGGACACAAGCTGTCTCCCATCTCTCGTGAATGCAGATTCCACACAACTCCTCAAACTGATGCTTGGCTTCCCTTGTCTGGTTCCGCTGGGGTTGAACTCCATTTTGCTAACAGCATACACCATCGTATTGCTGATAATGAGGAACCATCTTTTTGTTTGGAGCGTCTTTTCCCCAAAGTACATTTATGTTTGTACCACAACTATATGCGTCTACATGGGTGTATCTGTAGTAGCTACAACAGTGACATATACTTACTGGGTGCTGGGGTTGAGGAAAAAGATGCTATCTAAGCTTGGCCATCACCATTTAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016254 GO:0016740 GO:0016772 GO:0016780 GO:0019538 GO:0019637 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0051267 GO:0051377 GO:0071704 GO:0090407 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00563 map00563

Pfam Domains

Protein Families

Phosphodiest

Protein Analysis

985

Amino Acids

109.69

Weight (kDa)

7.17

Isoelectric Point (pI)

39.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphodiest	PF01663	220 - 298	2.4e-08	Type I phosphodiesterase / nucleotide pyrophosphatase
PIGO_PIGG	PF19316	526 - 963	6.2e-26	GPI ethanolamine phosphate transferase membrane region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22530
fragaria_vesca	FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740
malus_domestica	MD15G1313700.v1.1
prunus_persica	Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1
pyrus_communis	pycom15g27700
rosa_chinensis	RchiOBHm_Chr2g0111321
rosa_laevigata	RLG00000017886
rosa_multiflora	Rmu_sc0001363.1_g000002 Rmu_sc0005033.1_g000002 Rmu_ssc0000075.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132500
rosa_rugosa	Rorug02G0172800
rosa_samantha	Rh2AG225900 Rh2BG237700 Rh2CG228900 Rh2DG233400
rosa_wichuraiana	Rw2G017440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1833
AccB7I	CCANNNNNTGG	2 cut(s)	718, 1202
AccI	GTMKAC	3 cut(s)	823, 1515, 2850
AciI	CCGC	3 cut(s)	149, 236, 2723
AclI	AACGTT	1 cut(s)	1062
AclWI	GGATC	1 cut(s)	654
AcoI	YGGCCR	5 cut(s)	517, 2385, 2466, 2470, 2935
AcsI	RAATTY	7 cut(s)	478, 1083, 1132, 1681, 1988, 2379, 2565
AcuI	CTGAAG	2 cut(s)	1122, 1210
AcvI	CACGTG	2 cut(s)	643, 1199
AfaI	GTAC	9 cut(s)	165, 639, 2092, 2106, 2172, 2213, 2556, 2819, 2833
AfiI	CCNNNNNNNGG	8 cut(s)	394, 469, 718, 1202, 2142, 2163, 2617, 2716
AflII	CTTAAG	3 cut(s)	572, 1207, 1948
AflIII	ACRYGT	2 cut(s)	640, 2214
AjnI	CCWGG	7 cut(s)	462, 1002, 1136, 1171, 1304, 1325, 1562
AleI	CACNNNNGTG	1 cut(s)	151
Alw21I	GWGCWC	5 cut(s)	20, 148, 297, 1244, 1732
Alw26I	GTCTC	4 cut(s)	1571, 1930, 2635, 2657
Alw44I	GTGCAC	1 cut(s)	16
AlwI	GGATC	1 cut(s)	654
AoxI	GGCC	8 cut(s)	67, 517, 768, 2385, 2413, 2466, 2470, 2935
ApaLI	GTGCAC	1 cut(s)	16
ApeKI	GCWGC	7 cut(s)	398, 1269, 1286, 1454, 2254, 2329, 2444
ApoI	RAATTY	7 cut(s)	478, 1083, 1132, 1681, 1988, 2379, 2565
ArsI	GACNNNNNNTTYG	2 cut(s)	1565, 1597
AspLEI	GCGC	1 cut(s)	753
AspS9I	GGNCC	2 cut(s)	769, 909
AsuC2I	CCSGG	2 cut(s)	117, 585
AvaII	GGWCC	1 cut(s)	909
BaeGI	GKGCMC	1 cut(s)	20
BalI	TGGCCA	2 cut(s)	2387, 2937
BanI	GGYRCC	1 cut(s)	1833
BauI	CACGAG	2 cut(s)	290, 2663
BbrPI	CACGTG	2 cut(s)	643, 1199
BbsI	GAAGAC	5 cut(s)	76, 269, 1110, 1350, 2309
Bbv12I	GWGCWC	5 cut(s)	20, 148, 297, 1244, 1732
BbvI	GCAGC	7 cut(s)	385, 1256, 1273, 1441, 2241, 2316, 2431
BciT130I	CCWGG	7 cut(s)	464, 1004, 1138, 1173, 1306, 1327, 1564
BclI	TGATCA	2 cut(s)	970, 1216
BcnI	CCSGG	2 cut(s)	117, 585
BcoDI	GTCTC	4 cut(s)	1571, 1930, 2635, 2657
BfaI	CTAG	5 cut(s)	1353, 1922, 1937, 2285, 2501
BfmI	CTRYAG	3 cut(s)	1716, 2358, 2865
BfrI	CTTAAG	3 cut(s)	572, 1207, 1948
BglI	GCCNNNNNGGC	1 cut(s)	767
BglII	AGATCT	1 cut(s)	1735
BisI	GCNGC	8 cut(s)	236, 399, 1270, 1287, 1455, 2255, 2330, 2445
BlsI	GCNGC	8 cut(s)	237, 400, 1271, 1288, 1456, 2256, 2331, 2446
BmcAI	AGTACT	1 cut(s)	2556
Bme1390I	CCNGG	9 cut(s)	117, 464, 585, 1004, 1138, 1173, 1306, 1327, 1564
Bme18I	GGWCC	1 cut(s)	909
BmgT120I	GGNCC	2 cut(s)	769, 909
BmiI	GGNNCC	6 cut(s)	386, 771, 910, 1835, 2721, 2783
BmrFI	CCNGG	9 cut(s)	117, 464, 585, 1004, 1138, 1173, 1306, 1327, 1564
BmrI	ACTGGG	2 cut(s)	703, 2905
BmsI	GCATC	8 cut(s)	199, 540, 1560, 1631, 2428, 2600, 2688, 2910
BmuI	ACTGGG	2 cut(s)	703, 2905
BpiI	GAAGAC	5 cut(s)	76, 269, 1110, 1350, 2309
BpmI	CTGGAG	4 cut(s)	66, 465, 1145, 1309
Bpu10I	CCTNAGC	3 cut(s)	203, 934, 2612
BpuEI	CTTGAG	2 cut(s)	1904, 2218
BpuMI	CCSGG	2 cut(s)	117, 585
BsaAI	YACGTR	2 cut(s)	643, 1199
BsaBI	GATNNNNATC	2 cut(s)	189, 507
BsaJI	CCNNGG	5 cut(s)	5, 877, 1752, 1837, 2162
BsaXI	ACNNNNNCTCC	2 cut(s)	1722, 1752
Bsc4I	CCNNNNNNNGG	8 cut(s)	394, 469, 718, 1202, 2142, 2163, 2617, 2716
Bse118I	RCCGGY	1 cut(s)	2468
Bse1I	ACTGG	4 cut(s)	520, 698, 1128, 2900
Bse3DI	GCAATG	3 cut(s)	1011, 1464, 2435
Bse8I	GATNNNNATC	2 cut(s)	189, 507
BseBI	CCWGG	7 cut(s)	464, 1004, 1138, 1173, 1306, 1327, 1564
BseDI	CCNNGG	5 cut(s)	5, 877, 1752, 1837, 2162
BseGI	GGATG	8 cut(s)	190, 310, 472, 796, 1219, 1314, 1748, 2591
BseJI	GATNNNNATC	2 cut(s)	189, 507
BseLI	CCNNNNNNNGG	8 cut(s)	394, 469, 718, 1202, 2142, 2163, 2617, 2716
BseMI	GCAATG	3 cut(s)	1011, 1464, 2435
BseMII	CTCAG	4 cut(s)	217, 879, 1396, 2178
BseNI	ACTGG	4 cut(s)	520, 698, 1128, 2900
BseRI	GAGGAG	1 cut(s)	2678
BseSI	GKGCMC	1 cut(s)	20
BseXI	GCAGC	7 cut(s)	385, 1256, 1273, 1441, 2241, 2316, 2431
BseYI	CCCAGC	3 cut(s)	1892, 2725, 2902
BsgI	GTGCAG	2 cut(s)	384, 1272
BshFI	GGCC	8 cut(s)	69, 519, 770, 2387, 2415, 2468, 2472, 2937
BshNI	GGYRCC	1 cut(s)	1833
BsiHKAI	GWGCWC	5 cut(s)	20, 148, 297, 1244, 1732
BsiSI	CCGG	4 cut(s)	117, 585, 2469, 2520
BsiWI	CGTACG	1 cut(s)	2170
BslFI	GGGAC	3 cut(s)	1516, 1909, 2158
BslI	CCNNNNNNNGG	8 cut(s)	394, 469, 718, 1202, 2142, 2163, 2617, 2716
BsmAI	GTCTC	4 cut(s)	1571, 1930, 2635, 2657
BsmFI	GGGAC	3 cut(s)	1516, 1909, 2158
BsmI	GAATGC	2 cut(s)	237, 2674
BsnI	GGCC	8 cut(s)	69, 519, 770, 2387, 2415, 2468, 2472, 2937
Bsp1286I	GDGCHC	5 cut(s)	20, 148, 297, 1244, 1732
Bsp1407I	TGTACA	2 cut(s)	637, 2211
Bsp143I	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
Bsp19I	CCATGG	3 cut(s)	5, 877, 2162
BspACI	CCGC	3 cut(s)	149, 236, 2723
BspANI	GGCC	8 cut(s)	69, 519, 770, 2387, 2415, 2468, 2472, 2937
BspCNI	CTCAG	4 cut(s)	216, 880, 1395, 2179
BspHI	TCATGA	1 cut(s)	2203
BspLI	GGNNCC	6 cut(s)	386, 771, 910, 1835, 2721, 2783
BspPI	GGATC	1 cut(s)	654
BspQI	GCTCTTC	2 cut(s)	1035, 1638
BspT107I	GGYRCC	1 cut(s)	1833
BspTI	CTTAAG	3 cut(s)	572, 1207, 1948
BsrDI	GCAATG	3 cut(s)	1011, 1464, 2435
BsrFI	RCCGGY	1 cut(s)	2468
BsrGI	TGTACA	2 cut(s)	637, 2211
BsrI	ACTGG	4 cut(s)	520, 698, 1128, 2900
BssAI	RCCGGY	1 cut(s)	2468
BssECI	CCNNGG	5 cut(s)	5, 877, 1752, 1837, 2162
BssMI	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
BssNAI	GTATAC	1 cut(s)	824
BssSI	CACGAG	2 cut(s)	290, 2663
BssT1I	CCWWGG	5 cut(s)	5, 877, 1752, 1837, 2162
Bst1107I	GTATAC	1 cut(s)	824
Bst2BI	CACGAG	2 cut(s)	290, 2663
Bst2UI	CCWGG	7 cut(s)	464, 1004, 1138, 1173, 1306, 1327, 1564
Bst6I	CTCTTC	2 cut(s)	1035, 1638
BstAFI	CTTAAG	3 cut(s)	572, 1207, 1948
BstAPI	GCANNNNNTGC	2 cut(s)	398, 2329
BstAUI	TGTACA	2 cut(s)	637, 2211
BstBAI	YACGTR	2 cut(s)	643, 1199
BstC8I	GCNNGC	8 cut(s)	240, 363, 1161, 1832, 1890, 2389, 2417, 2470
BstDSI	CCRYGG	3 cut(s)	5, 877, 2162
BstF5I	GGATG	8 cut(s)	190, 310, 472, 796, 1219, 1314, 1748, 2591
BstHHI	GCGC	1 cut(s)	753
BstKTI	GATC	6 cut(s)	187, 649, 730, 973, 1219, 1738
BstMAI	GTCTC	4 cut(s)	1571, 1930, 2635, 2657
BstMBI	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
BstNI	CCWGG	7 cut(s)	464, 1004, 1138, 1173, 1306, 1327, 1564
BstNSI	RCATGY	3 cut(s)	1506, 2218, 2395
BstSCI	CCNGG	9 cut(s)	115, 462, 583, 1002, 1136, 1171, 1304, 1325, 1562
BstSFI	CTRYAG	3 cut(s)	1716, 2358, 2865
BstSLI	GKGCMC	1 cut(s)	20
BstV1I	GCAGC	7 cut(s)	385, 1256, 1273, 1441, 2241, 2316, 2431
BstV2I	GAAGAC	5 cut(s)	76, 269, 1110, 1350, 2309
BstX2I	RGATCY	1 cut(s)	1735
BstXI	CCANNNNNNTGG	1 cut(s)	1798
BstYI	RGATCY	1 cut(s)	1735
BstZ17I	GTATAC	1 cut(s)	824
BsuRI	GGCC	8 cut(s)	69, 519, 770, 2387, 2415, 2468, 2472, 2937
BtgI	CCRYGG	3 cut(s)	5, 877, 2162
BtgZI	GCGATG	1 cut(s)	1274
BtsCI	GGATG	8 cut(s)	190, 310, 472, 796, 1219, 1314, 1748, 2591
BtsI	GCAGTG	2 cut(s)	158, 356
BtsIMutI	CAGTG	6 cut(s)	130, 158, 356, 763, 1121, 2886
Cac8I	GCNNGC	8 cut(s)	240, 363, 1161, 1832, 1890, 2389, 2417, 2470
CciI	TCATGA	1 cut(s)	2203
CfoI	GCGC	1 cut(s)	753
Cfr10I	RCCGGY	1 cut(s)	2468
Cfr13I	GGNCC	2 cut(s)	769, 909
CseI	GACGC	4 cut(s)	37, 2064, 2791, 2836
CsiI	ACCWGGT	3 cut(s)	1002, 1171, 1562
Csp6I	GTAC	9 cut(s)	164, 638, 2091, 2105, 2171, 2212, 2555, 2818, 2832
CviQI	GTAC	9 cut(s)	164, 638, 2091, 2105, 2171, 2212, 2555, 2818, 2832
DpnI	GATC	6 cut(s)	186, 648, 729, 972, 1218, 1737
DpnII	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
EaeI	YGGCCR	5 cut(s)	517, 2385, 2466, 2470, 2935
Eam1104I	CTCTTC	2 cut(s)	1035, 1638
EarI	CTCTTC	2 cut(s)	1035, 1638
Eco130I	CCWWGG	5 cut(s)	5, 877, 1752, 1837, 2162
Eco47I	GGWCC	1 cut(s)	909
Eco57I	CTGAAG	2 cut(s)	1122, 1210
Eco72I	CACGTG	2 cut(s)	643, 1199
EcoO109I	RGGNCCY	1 cut(s)	909
EcoRI	GAATTC	1 cut(s)	1132
EcoRII	CCWGG	7 cut(s)	462, 1002, 1136, 1171, 1304, 1325, 1562
EcoT14I	CCWWGG	5 cut(s)	5, 877, 1752, 1837, 2162
EcoT22I	ATGCAT	1 cut(s)	2593
ErhI	CCWWGG	5 cut(s)	5, 877, 1752, 1837, 2162
FalI	AAGNNNNNCTT	2 cut(s)	1503, 1535
FaqI	GGGAC	3 cut(s)	1516, 1909, 2158
FauNDI	CATATG	1 cut(s)	813
FbaI	TGATCA	2 cut(s)	970, 1216
FblI	GTMKAC	3 cut(s)	823, 1515, 2850
Fnu4HI	GCNGC	8 cut(s)	236, 399, 1270, 1287, 1455, 2255, 2330, 2445
FokI	GGATG	8 cut(s)	177, 317, 479, 803, 1226, 1321, 1735, 2578
FseI	GGCCGGCC	1 cut(s)	2472
Fsp4HI	GCNGC	8 cut(s)	236, 399, 1270, 1287, 1455, 2255, 2330, 2445
FspBI	CTAG	5 cut(s)	1353, 1922, 1937, 2285, 2501
GlaI	GCGC	1 cut(s)	752
GluI	GCNGC	8 cut(s)	236, 399, 1270, 1287, 1455, 2255, 2330, 2445
GsaI	CCCAGC	3 cut(s)	1896, 2729, 2906
GsuI	CTGGAG	4 cut(s)	66, 465, 1145, 1309
HaeIII	GGCC	8 cut(s)	69, 519, 770, 2387, 2415, 2468, 2472, 2937
HapII	CCGG	4 cut(s)	117, 585, 2469, 2520
HgaI	GACGC	4 cut(s)	37, 2064, 2791, 2836
HhaI	GCGC	1 cut(s)	753
Hin6I	GCGC	1 cut(s)	751
HinP1I	GCGC	1 cut(s)	751
HincII	GTYRAC	2 cut(s)	1009, 2220
HindII	GTYRAC	2 cut(s)	1009, 2220
HindIII	AAGCTT	2 cut(s)	1951, 2930
HpaII	CCGG	4 cut(s)	117, 585, 2469, 2520
Hpy166II	GTNNAC	8 cut(s)	18, 640, 824, 984, 1009, 1516, 2220, 2851
Hpy8I	GTNNAC	8 cut(s)	18, 640, 824, 984, 1009, 1516, 2220, 2851
HpyAV	CCTTC	8 cut(s)	122, 424, 839, 1111, 1499, 1809, 1943, 2632
HpyCH4IV	ACGT	5 cut(s)	14, 642, 1062, 1198, 2173
HpySE526I	ACGT	5 cut(s)	14, 642, 1062, 1198, 2173
HspAI	GCGC	1 cut(s)	751
KroI	GCCGGC	1 cut(s)	2468
KroNI	GCCGGC	1 cut(s)	2470
Ksp22I	TGATCA	2 cut(s)	970, 1216
Kzo9I	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
LguI	GCTCTTC	2 cut(s)	1035, 1638
LmnI	GCTCC	6 cut(s)	446, 1248, 1467, 1727, 2522, 2799
Lsp1109I	GCAGC	7 cut(s)	385, 1256, 1273, 1441, 2241, 2316, 2431
LweI	GCATC	8 cut(s)	199, 540, 1560, 1631, 2428, 2600, 2688, 2910
MabI	ACCWGGT	3 cut(s)	1002, 1171, 1562
MaeI	CTAG	5 cut(s)	1353, 1922, 1937, 2285, 2501
MaeII	ACGT	5 cut(s)	14, 642, 1062, 1198, 2173
MaeIII	GTNAC	7 cut(s)	207, 354, 412, 872, 1404, 2486, 2881
MalI	GATC	6 cut(s)	186, 648, 729, 972, 1218, 1737
MboI	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
MfeI	CAATTG	1 cut(s)	378
MflI	RGATCY	1 cut(s)	1735
MhlI	GDGCHC	5 cut(s)	20, 148, 297, 1244, 1732
MlsI	TGGCCA	2 cut(s)	2387, 2937
MluNI	TGGCCA	2 cut(s)	2387, 2937
MlyI	GAGTC	3 cut(s)	618, 923, 2209
MmeI	TCCRAC	4 cut(s)	431, 518, 715, 1426
Mox20I	TGGCCA	2 cut(s)	2387, 2937
Mph1103I	ATGCAT	1 cut(s)	2593
MroNI	GCCGGC	1 cut(s)	2468
MscI	TGGCCA	2 cut(s)	2387, 2937
MslI	CAYNNNNRTG	5 cut(s)	151, 744, 1796, 2061, 2621
Msp20I	TGGCCA	2 cut(s)	2387, 2937
MspA1I	CMGCKG	1 cut(s)	2725
MspCI	CTTAAG	3 cut(s)	572, 1207, 1948
MspI	CCGG	4 cut(s)	117, 585, 2469, 2520
MspR9I	CCNGG	9 cut(s)	117, 464, 585, 1004, 1138, 1173, 1306, 1327, 1564
MunI	CAATTG	1 cut(s)	378
Mva1269I	GAATGC	2 cut(s)	237, 2674
MvaI	CCWGG	7 cut(s)	464, 1004, 1138, 1173, 1306, 1327, 1564
NaeI	GCCGGC	1 cut(s)	2470
NciI	CCSGG	2 cut(s)	117, 585
NcoI	CCATGG	3 cut(s)	5, 877, 2162
NdeI	CATATG	1 cut(s)	813
NdeII	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
NgoMIV	GCCGGC	1 cut(s)	2468
NlaIV	GGNNCC	6 cut(s)	386, 771, 910, 1835, 2721, 2783
NmeAIII	GCCGAG	2 cut(s)	276, 495
NmuCI	GTSAC	5 cut(s)	207, 354, 872, 1404, 2881
NsiI	ATGCAT	1 cut(s)	2593
NspI	RCATGY	3 cut(s)	1506, 2218, 2395
OliI	CACNNNNGTG	1 cut(s)	151
PagI	TCATGA	1 cut(s)	2203
PciI	ACATGT	1 cut(s)	2214
PciSI	GCTCTTC	2 cut(s)	1035, 1638
PctI	GAATGC	2 cut(s)	237, 2674
PdiI	GCCGGC	1 cut(s)	2470
Pfl23II	CGTACG	1 cut(s)	2170
PflMI	CCANNNNNTGG	2 cut(s)	718, 1202
PfoI	TCCNGGA	4 cut(s)	115, 462, 583, 1304
PkrI	GCNGC	8 cut(s)	237, 400, 1271, 1288, 1456, 2256, 2331, 2446
PleI	GAGTC	3 cut(s)	617, 923, 2208
PmaCI	CACGTG	2 cut(s)	643, 1199
PmlI	CACGTG	2 cut(s)	643, 1199
PpsI	GAGTC	3 cut(s)	617, 923, 2208
Ppu21I	YACGTR	2 cut(s)	643, 1199
PpuMI	RGGWCCY	1 cut(s)	909
PscI	ACATGT	1 cut(s)	2214
Psp1406I	AACGTT	1 cut(s)	1062
Psp5II	RGGWCCY	1 cut(s)	909
Psp6I	CCWGG	7 cut(s)	462, 1002, 1136, 1171, 1304, 1325, 1562
PspCI	CACGTG	2 cut(s)	643, 1199
PspFI	CCCAGC	3 cut(s)	1892, 2725, 2902
PspGI	CCWGG	7 cut(s)	462, 1002, 1136, 1171, 1304, 1325, 1562
PspLI	CGTACG	1 cut(s)	2170
PspN4I	GGNNCC	6 cut(s)	386, 771, 910, 1835, 2721, 2783
PspPI	GGNCC	2 cut(s)	769, 909
PspPPI	RGGWCCY	1 cut(s)	909
PsuI	RGATCY	1 cut(s)	1735
RigI	GGCCGGCC	1 cut(s)	2472
RsaI	GTAC	9 cut(s)	165, 639, 2092, 2106, 2172, 2213, 2556, 2819, 2833
RsaNI	GTAC	9 cut(s)	164, 638, 2091, 2105, 2171, 2212, 2555, 2818, 2832
RseI	CAYNNNNRTG	5 cut(s)	151, 744, 1796, 2061, 2621
SapI	GCTCTTC	2 cut(s)	1035, 1638
SatI	GCNGC	8 cut(s)	236, 399, 1270, 1287, 1455, 2255, 2330, 2445
Sau3AI	GATC	6 cut(s)	184, 646, 727, 970, 1216, 1735
Sau96I	GGNCC	2 cut(s)	769, 909
ScaI	AGTACT	1 cut(s)	2556
SchI	GAGTC	3 cut(s)	618, 923, 2209
ScrFI	CCNGG	9 cut(s)	117, 464, 585, 1004, 1138, 1173, 1306, 1327, 1564
SduI	GDGCHC	5 cut(s)	20, 148, 297, 1244, 1732
SexAI	ACCWGGT	3 cut(s)	1002, 1171, 1562
SfaNI	GCATC	8 cut(s)	199, 540, 1560, 1631, 2428, 2600, 2688, 2910
SfcI	CTRYAG	3 cut(s)	1716, 2358, 2865
SinI	GGWCC	1 cut(s)	909
SmiMI	CAYNNNNRTG	5 cut(s)	151, 744, 1796, 2061, 2621
SmlI	CTYRAG	5 cut(s)	572, 1207, 1883, 1948, 2197
SmoI	CTYRAG	5 cut(s)	572, 1207, 1883, 1948, 2197
SsiI	CCGC	3 cut(s)	149, 236, 2723
SspMI	CTAG	5 cut(s)	1353, 1922, 1937, 2285, 2501
StyD4I	CCNGG	9 cut(s)	115, 462, 583, 1002, 1136, 1171, 1304, 1325, 1562
StyI	CCWWGG	5 cut(s)	5, 877, 1752, 1837, 2162
TaiI	ACGT	5 cut(s)	17, 645, 1065, 1201, 2176
TaqI	TCGA	7 cut(s)	142, 661, 781, 1186, 1780, 2081, 2549
TaqII	GACCGA	2 cut(s)	264, 342
TatI	WGTACW	5 cut(s)	637, 2090, 2211, 2554, 2817
TauI	GCSGC	1 cut(s)	238
TscAI	CASTG	6 cut(s)	130, 158, 363, 763, 1128, 2886
TseFI	GTSAC	5 cut(s)	207, 354, 872, 1404, 2881
TseI	GCWGC	7 cut(s)	398, 1269, 1286, 1454, 2254, 2329, 2444
Tsp45I	GTSAC	5 cut(s)	207, 354, 872, 1404, 2881
TspGWI	ACGGA	1 cut(s)	918
TspRI	CASTG	6 cut(s)	130, 158, 363, 763, 1128, 2886
Van91I	CCANNNNNTGG	2 cut(s)	718, 1202
Vha464I	CTTAAG	3 cut(s)	572, 1207, 1948
VneI	GTGCAC	1 cut(s)	16
VpaK11BI	GGWCC	1 cut(s)	909
XapI	RAATTY	7 cut(s)	478, 1083, 1132, 1681, 1988, 2379, 2565
XceI	RCATGY	3 cut(s)	1506, 2218, 2395
XmiI	GTMKAC	3 cut(s)	823, 1515, 2850
XspI	CTAG	5 cut(s)	1353, 1922, 1937, 2285, 2501
ZrmI	AGTACT	1 cut(s)	2556
Zsp2I	ATGCAT	1 cut(s)	2593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.