Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0005351

Physical Location & Seq

Genomic Coordinates Forward (+)

22642848 .. 22649726

Gene Structure 6879 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017886

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGTCTGGGTTATCGTGCACGAAGCTGACGCTCTTCACCGTCGCCGGAGTTGCGATTCAACTCGTCGGCCTCTCTCTCTTCGTCTTCGGCTTCTTCCCCGTCAAGCCTACCCTTCCCGGCCACAGTGGTGCGGAGAGTTTTCGAGCACCCACTTGCAATTCGATTCGAAATGAGAGCCAGAATGATCTGCCTCCTCATCATCTCAGGTCACTCGATAAGGAACTATCTGGAATCCCACCTGCATTTGACAGGCTAATACTAATGGTTATTGATGGTCTTCCAGCTGAGTTTGTTCTAGGCAAGGATGGTAAGCCTCCAAGGAAGGATTTATTGGAGGCTATGCCGTATACTCAGTCATTGCTTGGAAATGGAATGGCAATCGGGTACCATGCAAAAGCCGCACCTCCAACTGTTACAATGCCTCGTTTGAAGGCTATGGTTTCTGGAGCAATTGGGGGATTCCTTGATGTGGCTTTGAATTTCAACACACAGGCTATGGTGGATGACAATCTTCTGGGCCAGTTTTTCAAGATTGGTTGGAAAATGGTGATGCTCGGTGATGAAACATGGCTCAAGTTGTTTCCCGGATTATTTATGAGGCATGATGGAGTCAGCAGTTTCTTCGTCAAAGATACTGTACAAGTGGATCAAAATGTTTCTAGACATCTGGGCAGTGAGCTTAGCAGAGATGACTGGAATTTTCTGATTCTTCATTATCTCGGCTTAGATCATGTTGGACATACTGGTGGGCGCAACAGTGCCTTGATGGCTCCAAAACTTACAGAGATGGATGAGGTGGTGAAGATGATTCATATGACTAATATACTGAATCAACAGAACGATCAAGGACAGACACTCTTGGTCGTAGTAAGTGACCACGGTATGACTGATAGTGGTAATCATGGAGGGTCCTCATATGAAGAAACGGACTCTTTAGCACTATTTATTGGCCTGAAAAATGATGTCTCTGATTATTTGTCATCTACGCATAACAATGTTGACCAGGTTGACATCGCACCAACTTTAGCTCTTCTATTAGGTGTGCCAATTCCAAAGAACAATGTTGGTGTTCTAATTCCAGAAATCTTTGGGTATTTGAGTGAAGACCAGCAGCTGAAGGCACTGGAGTTGAATTCCTGGCAATTAGTTAGATTATTGCAAGCACAAATACCTGGTTTATCATGTCAAAATCATCCCTGTGATGGCTCAGGTGGTGATCAAGAGTCCAGAGTAACTAAGTGTTATGGGAGTATAGAAAGGATGTGCTGTTGCTTATATACGAATGCTGCACTTCTCAAAAAGTCCTGGATGTCTAAGGAAGTTTCCAGGTCTAATAGTAGGAAAGACTACACCAGCACTGTTGCAGCATACTATGAGTTTCTGAGAACTGCAAGTGAGTGGCTATCACGCAGAGCAACTGATAAACCTGTAAGCCTGCTTACTCTTGGAATAGCTGCAATGCTCCTATCTTGTCTGATACTGTTAAGCCTTCTGTTTCACATCTACAAAGAAGTCTACACTAGAGAGATGCAATGCCCTTTTGACTTAAAGAGCATTATGCAGACCTGGTGTCTAGAAGAGACATTTATTTTGGGTGTTACATTGATTCTCATTATAAGTATGACATCAAGTTCTTTGGTAGAGGAAGAGCAGTATATTTGGCATTTTATGGCTTCCACATTGCATTTGTTACTTTTGCGAAAAGCAATACAAAGTTGTGTACACAGTTTTAACAGGGGACAAAACAAAACAAGTGGTCTTCAAATGAGTTCCATCTGTGTGCTTCTAATTTCTGGGAGGATATTGAGAGGTTGGCACCAAGGAGGCGTGAATTGGACCAATCTTCCTGACATATCTAAGTGGCTTGAGCAGGCTGGGAACGACAATGTAAAATCAGTTCAGCTAGTTGCATGCCTTCTAGTGATCGCCTTAAGCTTATATGCTCTTGTTTTATTTGGATCAAACAGGAATATAGTTCTGGTCATTGGATTTAGCTTTCTTGTGTCTGGGTTGTTGGTTGTGCAAAATATCCTTAAACATCAGGATGGCATGTTTGCCTCATCCAGTTGCAGTGCTACCACATTGGTACAGATAATTTATGCAATTCTTGGTGTTTCCACAAGTGGGACAGTTATTGCCTTGCCATGGCTTGTACCTTTTTCAATATCTGAGACTGGCTTGAGTCATGATGTGTACATGTCATCTGCTTCTAGTGAAGTTCAGAAGTCACTGCTGATGAAGTTAAGAGAATCTTTATTTCTAATTGGGTGGGCATACATATCCTGCTGGTGTCTTCTGCAGCTTTTGCTCCAACAAACAATTAACTCAATGCCTATACTCCTGCTACTTGTGCAAACTTTGGCCATCATGTTGTATTTTTCTTATAGTGGGTTGCACCACAACCGGTGGGTTGAGGTTTCTGCATTTTACTTTTTGGGAATGGCAGGCCATTTTGCTCTAGGAAACAGCAATTCTCTAGCTACTATTGATGTTGCCGGAGCCTTTATTGGCATCTCAAGTCACTCAACAGTACTTTCTGGAATTTTAATGTTCATTATAACCTATGCTTCCCCAATGCTATTCATTCTTAGCATGGTGATGTATATCTCTTTGAAGGACACAAGCTATTTTTTATCTCCGTCTGATGCAGATTTAAGACAAATCCTCAAAATGATGATTGGCTTTCCTTGTCTGGTTCCACTGGGCTTGAACTCCATTCTATTGACAGTATACACCATCGTATTGGTGTTTATGAGCAACCATCTTTTTGTCTGGAGCGTCTTTTCTCCAAAGTACATCTATGTTTGTACTACAACCATATGTGTGTATGTTGGAGTCTCTGTCATAGCTGCAACTGTAACACATGCATATTTGGTGCTGGGGTTAAGGAGAAAGAAGCAATATAAGCTTAGCCACCAGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016254 GO:0016740 GO:0016772 GO:0016780 GO:0019538 GO:0019637 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0051267 GO:0051377 GO:0071704 GO:0090407 GO:0098827 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00563 map00563

Pfam Domains

Protein Families

Phosphodiest

Protein Analysis

975

Amino Acids

107.64

Weight (kDa)

6.84

Isoelectric Point (pI)

43.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphodiest	PF01663	220 - 298	5.7e-08	Type I phosphodiesterase / nucleotide pyrophosphatase
PIGO_PIGG	PF19316	527 - 953	3.2e-27	GPI ethanolamine phosphate transferase membrane region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22530
fragaria_vesca	FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740 FvH4_1g19740
malus_domestica	MD15G1313700.v1.1
prunus_persica	Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1 Prupe.6G198700_v2.0.a1
pyrus_communis	pycom15g27700
rosa_chinensis	RchiOBHm_Chr2g0111321
rosa_laevigata	RLG00000017886
rosa_multiflora	Rmu_sc0001363.1_g000002 Rmu_sc0005033.1_g000002 Rmu_ssc0000075.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132500
rosa_rugosa	Rorug02G0172800
rosa_samantha	Rh2AG225900 Rh2BG237700 Rh2CG228900 Rh2DG233400
rosa_wichuraiana	Rw2G017440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1616, 2558
AarI	CACCTGC	1 cut(s)	247
Acc36I	ACCTGC	1 cut(s)	247
Acc65I	GGTACC	1 cut(s)	384
AccB1I	GGYRCC	2 cut(s)	384, 1815
AccI	GTMKAC	3 cut(s)	347, 1515, 2730
AciI	CCGC	2 cut(s)	131, 399
AclWI	GGATC	2 cut(s)	654, 1966
AcoI	YGGCCR	2 cut(s)	118, 2361
AcsI	RAATTY	4 cut(s)	478, 697, 1132, 2541
AcuI	CTGAAG	1 cut(s)	1136
AfaI	GTAC	9 cut(s)	386, 639, 1722, 2088, 2154, 2195, 2532, 2795, 2809
AfiI	CCNNNNNNNGG	4 cut(s)	469, 1202, 2124, 2145
AflII	CTTAAG	1 cut(s)	1930
AflIII	ACRYGT	1 cut(s)	2196
AgeI	ACCGGT	1 cut(s)	2403
AhdI	GACNNNNNGTC	1 cut(s)	1569
AjnI	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
AjuI	GAANNNNNNNTTGG	2 cut(s)	314, 346
AleI	CACNNNNGTG	1 cut(s)	126
Alw21I	GWGCWC	2 cut(s)	20, 148
Alw26I	GTCTC	4 cut(s)	970, 1574, 2165, 2842
Alw44I	GTGCAC	1 cut(s)	16
AlwI	GGATC	2 cut(s)	654, 1966
AlwNI	CAGNNNCTG	1 cut(s)	1114
AoxI	GGCC	6 cut(s)	67, 118, 517, 949, 2361, 2446
ApaLI	GTGCAC	1 cut(s)	16
ApeKI	GCWGC	6 cut(s)	1111, 1286, 1364, 1454, 2299, 2849
ApoI	RAATTY	4 cut(s)	478, 697, 1132, 2541
ArsI	GACNNNNNNTTYG	2 cut(s)	1573, 1605
AsiGI	ACCGGT	1 cut(s)	2403
Asp718I	GGTACC	1 cut(s)	384
AspLEI	GCGC	1 cut(s)	753
AspS9I	GGNCC	3 cut(s)	517, 909, 1836
AsuC2I	CCSGG	2 cut(s)	117, 585
AsuHPI	GGTGA	6 cut(s)	28, 559, 569, 811, 1226, 2608
AsuII	TTCGAA	1 cut(s)	166
AvaII	GGWCC	2 cut(s)	909, 1836
BaeGI	GKGCMC	1 cut(s)	20
BalI	TGGCCA	1 cut(s)	2363
BanI	GGYRCC	2 cut(s)	384, 1815
BbsI	GAAGAC	5 cut(s)	76, 269, 1110, 1751, 2285
Bbv12I	GWGCWC	2 cut(s)	20, 148
BbvI	GCAGC	6 cut(s)	1123, 1273, 1376, 1441, 2311, 2836
BceAI	ACGGC	1 cut(s)	328
BcgI	CGANNNNNNTGC	2 cut(s)	604, 638
BciT130I	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	2 cut(s)	117, 585
BcoDI	GTCTC	4 cut(s)	970, 1574, 2165, 2842
BfaI	CTAG	9 cut(s)	296, 660, 1521, 1574, 1904, 1919, 2211, 2459, 2477
BfmI	CTRYAG	1 cut(s)	2297
BfrI	CTTAAG	1 cut(s)	1930
BfuAI	ACCTGC	1 cut(s)	247
BglI	GCCNNNNNGGC	1 cut(s)	767
BisI	GCNGC	7 cut(s)	399, 1112, 1287, 1365, 1455, 2300, 2850
BlpI	GCTNAGC	2 cut(s)	680, 2909
BlsI	GCNGC	7 cut(s)	400, 1113, 1288, 1366, 1456, 2301, 2851
BmcAI	AGTACT	1 cut(s)	2532
Bme1390I	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
Bme18I	GGWCC	2 cut(s)	909, 1836
BmeRI	GACNNNNNGTC	1 cut(s)	1569
BmgT120I	GGNCC	3 cut(s)	517, 909, 1836
BmiI	GGNNCC	6 cut(s)	386, 771, 910, 1817, 2500, 2697
BmrFI	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
BmrI	ACTGGG	1 cut(s)	2711
BmsI	GCATC	4 cut(s)	540, 1518, 2520, 2635
BmuI	ACTGGG	1 cut(s)	2711
BpiI	GAAGAC	5 cut(s)	76, 269, 1110, 1751, 2285
BpmI	CTGGAG	3 cut(s)	465, 1145, 2794
Bpu10I	CCTNAGC	1 cut(s)	1207
Bpu1102I	GCTNAGC	2 cut(s)	680, 2909
Bpu14I	TTCGAA	1 cut(s)	166
BpuEI	CTTGAG	4 cut(s)	557, 1886, 2200, 2500
BpuMI	CCSGG	2 cut(s)	117, 585
BsaBI	GATNNNNATC	2 cut(s)	507, 2603
BsaJI	CCNNGG	4 cut(s)	317, 877, 1819, 2144
BsaWI	WCCGGW	1 cut(s)	2403
BsaXI	ACNNNNNCTCC	2 cut(s)	2826, 2856
Bsc4I	CCNNNNNNNGG	4 cut(s)	469, 1202, 2124, 2145
Bse118I	RCCGGY	1 cut(s)	2403
Bse1I	ACTGG	7 cut(s)	520, 698, 748, 1128, 2064, 2179, 2706
Bse3DI	GCAATG	4 cut(s)	356, 1464, 1538, 1679
Bse8I	GATNNNNATC	2 cut(s)	507, 2603
BseBI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
BseDI	CCNNGG	4 cut(s)	317, 877, 1819, 2144
BseGI	GGATG	8 cut(s)	310, 508, 796, 1192, 1266, 1314, 2050, 2060
BseJI	GATNNNNATC	2 cut(s)	507, 2603
BseLI	CCNNNNNNNGG	4 cut(s)	469, 1202, 2124, 2145
BseMI	GCAATG	4 cut(s)	356, 1464, 1538, 1679
BseMII	CTCAG	6 cut(s)	217, 276, 365, 1221, 1373, 2160
BseNI	ACTGG	7 cut(s)	520, 698, 748, 1128, 2064, 2179, 2706
BseRI	GAGGAG	1 cut(s)	183
BseSI	GKGCMC	1 cut(s)	20
BseXI	GCAGC	6 cut(s)	1123, 1273, 1376, 1441, 2311, 2836
BseYI	CCCAGC	2 cut(s)	1874, 2878
BsgI	GTGCAG	1 cut(s)	1272
BshFI	GGCC	6 cut(s)	69, 120, 519, 951, 2363, 2448
BshNI	GGYRCC	2 cut(s)	384, 1815
BshTI	ACCGGT	1 cut(s)	2403
BsiHKAI	GWGCWC	2 cut(s)	20, 148
BsiSI	CCGG	5 cut(s)	45, 117, 585, 2404, 2496
BslFI	GGGAC	2 cut(s)	1752, 2140
BslI	CCNNNNNNNGG	4 cut(s)	469, 1202, 2124, 2145
BsmAI	GTCTC	4 cut(s)	970, 1574, 2165, 2842
BsmFI	GGGAC	2 cut(s)	1752, 2140
BsmI	GAATGC	1 cut(s)	1288
BsnI	GGCC	6 cut(s)	69, 120, 519, 951, 2363, 2448
Bsp119I	TTCGAA	1 cut(s)	166
Bsp1286I	GDGCHC	2 cut(s)	20, 148
Bsp1407I	TGTACA	3 cut(s)	637, 1720, 2193
Bsp143I	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
Bsp1720I	GCTNAGC	2 cut(s)	680, 2909
Bsp19I	CCATGG	1 cut(s)	2144
BspACI	CCGC	2 cut(s)	131, 399
BspANI	GGCC	6 cut(s)	69, 120, 519, 951, 2363, 2448
BspCNI	CTCAG	6 cut(s)	216, 277, 364, 1220, 1374, 2161
BspHI	TCATGA	1 cut(s)	2185
BspLI	GGNNCC	6 cut(s)	386, 771, 910, 1817, 2500, 2697
BspMAI	CTGCAG	1 cut(s)	2301
BspMI	ACCTGC	1 cut(s)	247
BspPI	GGATC	2 cut(s)	654, 1966
BspQI	GCTCTTC	3 cut(s)	38, 1035, 1641
BspT104I	TTCGAA	1 cut(s)	166
BspT107I	GGYRCC	2 cut(s)	384, 1815
BspTI	CTTAAG	1 cut(s)	1930
BsrDI	GCAATG	4 cut(s)	356, 1464, 1538, 1679
BsrFI	RCCGGY	1 cut(s)	2403
BsrGI	TGTACA	3 cut(s)	637, 1720, 2193
BsrI	ACTGG	7 cut(s)	520, 698, 748, 1128, 2064, 2179, 2706
BssAI	RCCGGY	1 cut(s)	2403
BssECI	CCNNGG	4 cut(s)	317, 877, 1819, 2144
BssMI	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
BssNAI	GTATAC	2 cut(s)	348, 2731
BssT1I	CCWWGG	3 cut(s)	317, 1819, 2144
Bst1107I	GTATAC	2 cut(s)	348, 2731
Bst2UI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
Bst6I	CTCTTC	5 cut(s)	38, 83, 1035, 1572, 1641
BstAFI	CTTAAG	1 cut(s)	1930
BstAPI	GCANNNNNTGC	1 cut(s)	2305
BstAUI	TGTACA	3 cut(s)	637, 1720, 2193
BstBI	TTCGAA	1 cut(s)	166
BstC8I	GCNNGC	5 cut(s)	1161, 1436, 1872, 1912, 2446
BstDSI	CCRYGG	2 cut(s)	877, 2144
BstF5I	GGATG	8 cut(s)	310, 508, 796, 1192, 1266, 1314, 2050, 2060
BstHHI	GCGC	1 cut(s)	753
BstKTI	GATC	7 cut(s)	187, 649, 730, 844, 1219, 1926, 1961
BstMAI	GTCTC	4 cut(s)	970, 1574, 2165, 2842
BstMBI	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
BstMWI	GCNNNNNNNGC	6 cut(s)	50, 398, 767, 2305, 2350, 2905
BstNI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
BstNSI	RCATGY	4 cut(s)	1914, 2053, 2200, 2867
BstSCI	CCNGG	8 cut(s)	115, 583, 1002, 1136, 1171, 1304, 1325, 1565
BstSFI	CTRYAG	1 cut(s)	2297
BstSLI	GKGCMC	1 cut(s)	20
BstV1I	GCAGC	6 cut(s)	1123, 1273, 1376, 1441, 2311, 2836
BstV2I	GAAGAC	5 cut(s)	76, 269, 1110, 1751, 2285
BstXI	CCANNNNNNTGG	1 cut(s)	2743
BstZ17I	GTATAC	2 cut(s)	348, 2731
BsuRI	GGCC	6 cut(s)	69, 120, 519, 951, 2363, 2448
BtgI	CCRYGG	2 cut(s)	877, 2144
BtgZI	GCGATG	1 cut(s)	997
BtsCI	GGATG	8 cut(s)	310, 508, 796, 1192, 1266, 1314, 2050, 2060
BtsI	GCAGTG	3 cut(s)	679, 2077, 2228
BtsIMutI	CAGTG	8 cut(s)	130, 679, 763, 1121, 1356, 2077, 2228, 2699
BveI	ACCTGC	1 cut(s)	247
Cac8I	GCNNGC	5 cut(s)	1161, 1436, 1872, 1912, 2446
CaiI	CAGNNNCTG	1 cut(s)	1114
CciI	TCATGA	1 cut(s)	2185
CfoI	GCGC	1 cut(s)	753
Cfr10I	RCCGGY	1 cut(s)	2403
Cfr13I	GGNCC	3 cut(s)	517, 909, 1836
CseI	GACGC	2 cut(s)	37, 2767
CsiI	ACCWGGT	3 cut(s)	1002, 1171, 1565
Csp6I	GTAC	9 cut(s)	385, 638, 1721, 2087, 2153, 2194, 2531, 2794, 2808
CspAI	ACCGGT	1 cut(s)	2403
CspCI	CAANNNNNGTGG	4 cut(s)	1735, 1770, 2688, 2723
CviQI	GTAC	9 cut(s)	385, 638, 1721, 2087, 2153, 2194, 2531, 2794, 2808
DpnI	GATC	7 cut(s)	186, 648, 729, 843, 1218, 1925, 1960
DpnII	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
DriI	GACNNNNNGTC	1 cut(s)	1569
EaeI	YGGCCR	2 cut(s)	118, 2361
Eam1104I	CTCTTC	5 cut(s)	38, 83, 1035, 1572, 1641
Eam1105I	GACNNNNNGTC	1 cut(s)	1569
EarI	CTCTTC	5 cut(s)	38, 83, 1035, 1572, 1641
Eco130I	CCWWGG	3 cut(s)	317, 1819, 2144
Eco47I	GGWCC	2 cut(s)	909, 1836
Eco57I	CTGAAG	1 cut(s)	1136
EcoO109I	RGGNCCY	1 cut(s)	909
EcoRI	GAATTC	1 cut(s)	1132
EcoRII	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
EcoT14I	CCWWGG	3 cut(s)	317, 1819, 2144
EcoT22I	ATGCAT	1 cut(s)	2869
ErhI	CCWWGG	3 cut(s)	317, 1819, 2144
FalI	AAGNNNNNCTT	2 cut(s)	2236, 2268
FaqI	GGGAC	2 cut(s)	1752, 2140
FauNDI	CATATG	3 cut(s)	813, 916, 2819
FbaI	TGATCA	1 cut(s)	1216
FblI	GTMKAC	3 cut(s)	347, 1515, 2730
Fnu4HI	GCNGC	7 cut(s)	399, 1112, 1287, 1365, 1455, 2300, 2850
FokI	GGATG	8 cut(s)	317, 515, 803, 1179, 1273, 1321, 2047, 2057
Fsp4HI	GCNGC	7 cut(s)	399, 1112, 1287, 1365, 1455, 2300, 2850
FspBI	CTAG	9 cut(s)	296, 660, 1521, 1574, 1904, 1919, 2211, 2459, 2477
GlaI	GCGC	1 cut(s)	752
GluI	GCNGC	7 cut(s)	399, 1112, 1287, 1365, 1455, 2300, 2850
GsaI	CCCAGC	2 cut(s)	1878, 2882
GsuI	CTGGAG	3 cut(s)	465, 1145, 2794
HaeIII	GGCC	6 cut(s)	69, 120, 519, 951, 2363, 2448
HapII	CCGG	5 cut(s)	45, 117, 585, 2404, 2496
HgaI	GACGC	2 cut(s)	37, 2767
HhaI	GCGC	1 cut(s)	753
Hin6I	GCGC	1 cut(s)	751
HinP1I	GCGC	1 cut(s)	751
HincII	GTYRAC	2 cut(s)	1000, 1009
HindII	GTYRAC	2 cut(s)	1000, 1009
HindIII	AAGCTT	2 cut(s)	1933, 2906
HpaII	CCGG	5 cut(s)	45, 117, 585, 2404, 2496
HphI	GGTGA	6 cut(s)	28, 559, 569, 811, 1226, 2608
Hpy166II	GTNNAC	9 cut(s)	18, 348, 1000, 1009, 1516, 1721, 1723, 2194, 2731
Hpy188I	TCNGA	7 cut(s)	705, 970, 1383, 1476, 2170, 2223, 2644
Hpy8I	GTNNAC	9 cut(s)	18, 348, 1000, 1009, 1516, 1721, 1723, 2194, 2731
Hpy99I	CGWCG	2 cut(s)	44, 68
HpyAV	CCTTC	7 cut(s)	122, 316, 424, 1111, 1499, 1925, 2608
HpyF10VI	GCNNNNNNNGC	6 cut(s)	50, 398, 767, 2305, 2350, 2905
HspAI	GCGC	1 cut(s)	751
KpnI	GGTACC	1 cut(s)	388
Ksp22I	TGATCA	1 cut(s)	1216
Kzo9I	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
LguI	GCTCTTC	3 cut(s)	38, 1035, 1641
LmnI	GCTCC	6 cut(s)	446, 775, 1467, 2313, 2498, 2775
Lsp1109I	GCAGC	6 cut(s)	1123, 1273, 1376, 1441, 2311, 2836
LweI	GCATC	4 cut(s)	540, 1518, 2520, 2635
MabI	ACCWGGT	3 cut(s)	1002, 1171, 1565
MaeI	CTAG	9 cut(s)	296, 660, 1521, 1574, 1904, 1919, 2211, 2459, 2477
MaeIII	GTNAC	9 cut(s)	207, 412, 872, 1231, 1597, 1689, 2226, 2519, 2857
MalI	GATC	7 cut(s)	186, 648, 729, 843, 1218, 1925, 1960
MboI	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
MfeI	CAATTG	1 cut(s)	450
MhlI	GDGCHC	2 cut(s)	20, 148
MlsI	TGGCCA	1 cut(s)	2363
MluNI	TGGCCA	1 cut(s)	2363
MlyI	GAGTC	5 cut(s)	618, 923, 1232, 2191, 2844
MmeI	TCCRAC	5 cut(s)	431, 518, 715, 2335, 2812
Mox20I	TGGCCA	1 cut(s)	2363
Mph1103I	ATGCAT	1 cut(s)	2869
MscI	TGGCCA	1 cut(s)	2363
MslI	CAYNNNNRTG	8 cut(s)	126, 744, 993, 1197, 1778, 2043, 2597, 2822
Msp20I	TGGCCA	1 cut(s)	2363
MspA1I	CMGCKG	2 cut(s)	284, 1114
MspCI	CTTAAG	1 cut(s)	1930
MspI	CCGG	5 cut(s)	45, 117, 585, 2404, 2496
MspR9I	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
MunI	CAATTG	1 cut(s)	450
Mva1269I	GAATGC	1 cut(s)	1288
MvaI	CCWGG	6 cut(s)	1004, 1138, 1173, 1306, 1327, 1567
MwoI	GCNNNNNNNGC	6 cut(s)	50, 398, 767, 2305, 2350, 2905
NciI	CCSGG	2 cut(s)	117, 585
NcoI	CCATGG	1 cut(s)	2144
NdeI	CATATG	3 cut(s)	813, 916, 2819
NdeII	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
NlaIV	GGNNCC	6 cut(s)	386, 771, 910, 1817, 2500, 2697
NmeAIII	GCCGAG	1 cut(s)	699
NmuCI	GTSAC	4 cut(s)	207, 872, 2226, 2519
NsiI	ATGCAT	1 cut(s)	2869
NspI	RCATGY	4 cut(s)	1914, 2053, 2200, 2867
NspV	TTCGAA	1 cut(s)	166
OliI	CACNNNNGTG	1 cut(s)	126
PaeI	GCATGC	1 cut(s)	1914
PagI	TCATGA	1 cut(s)	2185
PaqCI	CACCTGC	1 cut(s)	247
PciI	ACATGT	1 cut(s)	2196
PciSI	GCTCTTC	3 cut(s)	38, 1035, 1641
PctI	GAATGC	1 cut(s)	1288
PfeI	GAWTC	9 cut(s)	55, 163, 231, 459, 706, 808, 829, 1606, 2249
PfoI	TCCNGGA	2 cut(s)	583, 1304
PinAI	ACCGGT	1 cut(s)	2403
PkrI	GCNGC	7 cut(s)	400, 1113, 1288, 1366, 1456, 2301, 2851
PleI	GAGTC	5 cut(s)	617, 923, 1231, 2190, 2843
PpsI	GAGTC	5 cut(s)	617, 923, 1231, 2190, 2843
PpuMI	RGGWCCY	1 cut(s)	909
PscI	ACATGT	1 cut(s)	2196
PsiI	TTATAA	2 cut(s)	1616, 2558
Psp5II	RGGWCCY	1 cut(s)	909
Psp6I	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
PspFI	CCCAGC	2 cut(s)	1874, 2878
PspGI	CCWGG	6 cut(s)	1002, 1136, 1171, 1304, 1325, 1565
PspN4I	GGNNCC	6 cut(s)	386, 771, 910, 1817, 2500, 2697
PspPI	GGNCC	3 cut(s)	517, 909, 1836
PspPPI	RGGWCCY	1 cut(s)	909
PsrI	GAACNNNNNNTAC	2 cut(s)	1881, 1913
PstI	CTGCAG	1 cut(s)	2301
PstNI	CAGNNNCTG	1 cut(s)	1114
PvuII	CAGCTG	2 cut(s)	284, 1114
RsaI	GTAC	9 cut(s)	386, 639, 1722, 2088, 2154, 2195, 2532, 2795, 2809
RsaNI	GTAC	9 cut(s)	385, 638, 1721, 2087, 2153, 2194, 2531, 2794, 2808
RseI	CAYNNNNRTG	8 cut(s)	126, 744, 993, 1197, 1778, 2043, 2597, 2822
SapI	GCTCTTC	3 cut(s)	38, 1035, 1641
SatI	GCNGC	7 cut(s)	399, 1112, 1287, 1365, 1455, 2300, 2850
Sau3AI	GATC	7 cut(s)	184, 646, 727, 841, 1216, 1923, 1958
Sau96I	GGNCC	3 cut(s)	517, 909, 1836
ScaI	AGTACT	1 cut(s)	2532
SchI	GAGTC	5 cut(s)	618, 923, 1232, 2191, 2844
ScrFI	CCNGG	8 cut(s)	117, 585, 1004, 1138, 1173, 1306, 1327, 1567
SduI	GDGCHC	2 cut(s)	20, 148
SexAI	ACCWGGT	3 cut(s)	1002, 1171, 1565
SfaNI	GCATC	4 cut(s)	540, 1518, 2520, 2635
SfcI	CTRYAG	1 cut(s)	2297
SfuI	TTCGAA	1 cut(s)	166
SinI	GGWCC	2 cut(s)	909, 1836
SmiMI	CAYNNNNRTG	8 cut(s)	126, 744, 993, 1197, 1778, 2043, 2597, 2822
SmlI	CTYRAG	5 cut(s)	572, 1865, 1930, 2179, 2515
SmoI	CTYRAG	5 cut(s)	572, 1865, 1930, 2179, 2515
SphI	GCATGC	1 cut(s)	1914
SsiI	CCGC	2 cut(s)	131, 399
SspMI	CTAG	9 cut(s)	296, 660, 1521, 1574, 1904, 1919, 2211, 2459, 2477
StyD4I	CCNGG	8 cut(s)	115, 583, 1002, 1136, 1171, 1304, 1325, 1565
StyI	CCWWGG	3 cut(s)	317, 1819, 2144
TaqI	TCGA	4 cut(s)	142, 161, 166, 213
TatI	WGTACW	6 cut(s)	637, 1720, 2193, 2530, 2793, 2807
TauI	GCSGC	1 cut(s)	401
TfiI	GAWTC	9 cut(s)	55, 163, 231, 459, 706, 808, 829, 1606, 2249
TscAI	CASTG	8 cut(s)	130, 679, 763, 1128, 1363, 2077, 2235, 2706
TseFI	GTSAC	4 cut(s)	207, 872, 2226, 2519
TseI	GCWGC	6 cut(s)	1111, 1286, 1364, 1454, 2299, 2849
Tsp45I	GTSAC	4 cut(s)	207, 872, 2226, 2519
TspDTI	ATGAA	7 cut(s)	576, 701, 800, 933, 2252, 2542, 2572
TspGWI	ACGGA	2 cut(s)	941, 2628
TspRI	CASTG	8 cut(s)	130, 679, 763, 1128, 1363, 2077, 2235, 2706
Vha464I	CTTAAG	1 cut(s)	1930
VneI	GTGCAC	1 cut(s)	16
VpaK11BI	GGWCC	2 cut(s)	909, 1836
XapI	RAATTY	4 cut(s)	478, 697, 1132, 2541
XbaI	TCTAGA	2 cut(s)	659, 1573
XceI	RCATGY	4 cut(s)	1914, 2053, 2200, 2867
XmiI	GTMKAC	3 cut(s)	347, 1515, 2730
XspI	CTAG	9 cut(s)	296, 660, 1521, 1574, 1904, 1919, 2211, 2459, 2477
ZrmI	AGTACT	1 cut(s)	2532
Zsp2I	ATGCAT	1 cut(s)	2869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.