Rorug02G0172800

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001181
Physical Location & Seq
Reverse (-)
15718672 .. 15721805
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0172800.1

Sequence Viewer

Length: 1701 bp
ATGGATTTGTCACAAACTGGGAGAGCTGGGCTGAAAGCTCTCTATGTGGTGTTTGCTTTCTGCTCTGCTCTCTTTCTGGGTTTTCTCAAAAGTGTTCTTGTGGGTCCTGTTTCTGCTCTAATAATGATTATAGGGAATGTGGGAATCATATTGGGCTTGTTTCCTGCACATGTTGCCTGGATAATCTACACTCTTATAAAGATTGATAGATTTGATGTACCACTTAAAGTTGCAATATTGTTTGGCTTGCCTGCCCTGTTTGGTATCTGGCTGGGTTTGAGCATAGCTGGGAGTGTTCTTGTGGGATTGGGCTATGGGTTCTTCACCCCTTGGGTTTCTACTTTTGAGGCTTTTAGGCATGGCAATGAGTTGAAGAAGTTCAAACATTGTGTTATTGATGGAACTTGGGGAACTGTTAAAGGAAGCTGTACTGTAGTTAGAGACTTTGCAGACATATGTTACCACTCGTACCCGCTCTACTTGAAGGAGTTGCGTGAATCCCCTGCTTCAGAAGAAGTTAAAACTCTAAGGTTGATTCATGTACCAGCATGTATACTAGTTGGTGTGCTGGGGCTAATGGTGGAGATTCCTCTTTACACTGCAATTGCTGTTGTGAAGAGTCCATATATGCTGTTTAAGGGCTGGTATCGACTGATTCATGATCTGATTAGTCGAGAAGGACCATTTCTTGAAGTAGCTTGCATCCCTATTGCTGGTTTGACAATCCTCATGTGGCCAATTGTGGTTGTTGGAAGCATCTTACTGACAGTTGTCTCCAGCATTTTTGTTGGATTATATGCAGCAGTGGTAGTATATCAGGAAAGATCTTTCAAGAGAGGTGTGACTTACGTGATTGCAATGGTTGCTGAGTTTGATGAGTACACAAATGACTGGTTATATCTTCGAGAGGGCACCATCTTTCCGAAGCCCCATTATCGAAAAAGAGTTGTTTCTCAACCATCAGAGCTTTCTGTCCGAGGAAGGCTGGCAGCTGGAGGTAAGCTCAGTTCTGCTCCAATGGAGGCACCTGCAATGCATATGCCAAGTGCGGCCAATTCAAGATCAGTTAGAGAGGCTATACATGAAGTAAAAATGGTACAGATATGGGGACATATGATGAGATCCTGCGAACTGAGGGCTAAGCAACTGTTGGATGCAGAAGTTATAACCCCAAGTGATCTCTATGAATGGTTGAAATCTGAGAACAGCAATGAAGGGTGCATCATTGGTGTTGGTTTGCCTTGTTATTCATTCTTGCACACACTTCTCTGCTCCATCAGATCAAATTCGGCTGGTTTGCTGCTGCTTGATGATGTTGAAGTTACCTATTTGAATAGACCACATGACAAACTGATGGACTGGTTCTTTAATCCTATAATGGTCCTGAAGGAACAGGTCAAGGTCATAAAATTGGAAGAGGGTGAAGTGAGATTTCTGGAGAAAGTACTACTTTTCAGAAGCAATACACAGCGCATGGAGGCTTGGGATAATGGAAGTTTAATACCTCAAGATGCTCTAAGAGCTGCTCAAATCGAAGGCATTAGTAGAAGGATGATTGGGATGATGAGAGGTGTGTCAAAATTTCCCACTTATAGGAGGAGATTTCACCAAGTTGTGAAGAACTTGATCACATATTCCATACATAAGGAGTCTACTGTTGCGCCCAGTTCCATCGAATCTACCTCTACCGAAGATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

566

Amino Acids

63.36

Weight (kDa)

8.74

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 197, 1166
AarI CACCTGC 1 cut(s) 1036
Acc36I ACCTGC 1 cut(s) 1036
AccB1I GGYRCC 2 cut(s) 911, 1024
AccBSI CCGCTC 1 cut(s) 475
AccI GTMKAC 2 cut(s) 553, 1652
AciI CCGC 2 cut(s) 473, 1049
AclWI GGATC 1 cut(s) 1116
AcoI YGGCCR 2 cut(s) 734, 1050
AcsI RAATTY 2 cut(s) 1285, 1580
AcuI CTGAAG 2 cut(s) 492, 1406
AfaI GTAC 7 cut(s) 219, 430, 470, 543, 881, 1098, 1446
AfiI CCNNNNNNNGG 2 cut(s) 713, 1593
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 9 cut(s) 373, 382, 484, 692, 832, 1059, 1195, 1319, 1333
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 9 cut(s) 26, 38, 287, 426, 698, 967, 992, 1003, 1523
AluI AGCT 9 cut(s) 26, 38, 287, 426, 698, 967, 992, 1003, 1523
Alw26I GTCTC 2 cut(s) 435, 778
AlwI GGATC 1 cut(s) 1116
AoxI GGCC 2 cut(s) 734, 1050
ApeKI GCWGC 5 cut(s) 800, 989, 1300, 1303, 1523
ApoI RAATTY 2 cut(s) 1285, 1580
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 2 cut(s) 1473, 1663
AspS9I GGNCC 3 cut(s) 104, 680, 1381
AsuHPI GGTGA 3 cut(s) 316, 1433, 1598
AvaII GGWCC 3 cut(s) 104, 680, 1381
BaeGI GKGCMC 1 cut(s) 914
BalI TGGCCA 1 cut(s) 736
BanI GGYRCC 2 cut(s) 911, 1024
BbvI GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
BccI CCATC 6 cut(s) 392, 923, 967, 1283, 1348, 1679
BciT130I CCWGG 1 cut(s) 178
BclI TGATCA 1 cut(s) 1626
BcoDI GTCTC 2 cut(s) 435, 778
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 1036
BglII AGATCT 1 cut(s) 824
BisI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
BlpI GCTNAGC 1 cut(s) 1140
BlsI GCNGC 6 cut(s) 802, 991, 1051, 1302, 1305, 1525
BmcAI AGTACT 1 cut(s) 1446
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 3 cut(s) 104, 680, 1381
BmgT120I GGNCC 3 cut(s) 104, 680, 1381
BmiI GGNNCC 3 cut(s) 105, 913, 1026
BmrFI CCNGG 1 cut(s) 178
BmrI ACTGGG 2 cut(s) 27, 1659
BmsI GCATC 5 cut(s) 711, 765, 1144, 1230, 1501
BmuI ACTGGG 2 cut(s) 27, 1659
BoxI GACNNNNGTC 1 cut(s) 770
BplI GAGNNNNNCTC 2 cut(s) 987, 1019
BpmI CTGGAG 3 cut(s) 760, 1014, 1457
Bpu1102I GCTNAGC 1 cut(s) 1140
BpuEI CTTGAG 1 cut(s) 1491
BsaAI YACGTR 1 cut(s) 850
BsaJI CCNNGG 2 cut(s) 329, 976
Bsc4I CCNNNNNNNGG 2 cut(s) 713, 1593
Bse1I ACTGG 4 cut(s) 22, 896, 1364, 1665
Bse3DI GCAATG 4 cut(s) 370, 864, 1038, 1216
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 2 cut(s) 329, 976
BseGI GGATG 4 cut(s) 702, 1159, 1557, 1566
BseLI CCNNNNNNNGG 2 cut(s) 713, 1593
BseMI GCAATG 4 cut(s) 370, 864, 1038, 1216
BseMII CTCAG 4 cut(s) 858, 1018, 1124, 1191
BseNI ACTGG 4 cut(s) 22, 896, 1364, 1665
BseRI GAGGAG 1 cut(s) 1612
BseSI GKGCMC 1 cut(s) 914
BseXI GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
BseYI CCCAGC 4 cut(s) 26, 271, 287, 568
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 2 cut(s) 736, 1052
BshNI GGYRCC 2 cut(s) 911, 1024
BslFI GGGAC 1 cut(s) 1122
BslI CCNNNNNNNGG 2 cut(s) 713, 1593
BsmAI GTCTC 2 cut(s) 435, 778
BsmFI GGGAC 1 cut(s) 1122
BsnI GGCC 2 cut(s) 736, 1052
Bsp1286I GDGCHC 1 cut(s) 914
Bsp143I GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
Bsp1720I GCTNAGC 1 cut(s) 1140
BspACI CCGC 2 cut(s) 473, 1049
BspANI GGCC 2 cut(s) 736, 1052
BspCNI CTCAG 4 cut(s) 859, 1017, 1125, 1192
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 3 cut(s) 105, 913, 1026
BspMI ACCTGC 1 cut(s) 1036
BspPI GGATC 1 cut(s) 1116
BspT107I GGYRCC 2 cut(s) 911, 1024
BsrBI CCGCTC 1 cut(s) 475
BsrDI GCAATG 4 cut(s) 370, 864, 1038, 1216
BsrI ACTGG 4 cut(s) 22, 896, 1364, 1665
BssECI CCNNGG 2 cut(s) 329, 976
BssMI GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
BssNAI GTATAC 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 329
Bst1107I GTATAC 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 5 cut(s) 415, 433, 769, 1149, 1657
Bst6I CTCTTC 2 cut(s) 611, 1410
BstAPI GCANNNNNTGC 2 cut(s) 173, 863
BstBAI YACGTR 1 cut(s) 850
BstC8I GCNNGC 4 cut(s) 248, 252, 700, 987
BstDEI CTNAG 7 cut(s) 527, 867, 1004, 1133, 1140, 1200, 1517
BstF5I GGATG 4 cut(s) 702, 1159, 1557, 1566
BstHHI GCGC 2 cut(s) 1473, 1663
BstKTI GATC 7 cut(s) 664, 827, 1064, 1124, 1180, 1283, 1629
BstMAI GTCTC 2 cut(s) 435, 778
BstMBI GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
BstMWI GCNNNNNNNGC 3 cut(s) 173, 863, 1520
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 2 cut(s) 173, 552
BstPAI GACNNNNGTC 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 1 cut(s) 914
BstV1I GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
BstX2I RGATCY 2 cut(s) 824, 1121
BstYI RGATCY 2 cut(s) 824, 1121
BstZ17I GTATAC 1 cut(s) 554
BsuRI GGCC 2 cut(s) 736, 1052
BtsCI GGATG 4 cut(s) 702, 1159, 1557, 1566
BtsI GCAGTG 2 cut(s) 597, 810
BtsIMutI CAGTG 2 cut(s) 597, 810
BveI ACCTGC 1 cut(s) 1036
Cac8I GCNNGC 4 cut(s) 248, 252, 700, 987
CciI TCATGA 1 cut(s) 658
CfoI GCGC 2 cut(s) 1473, 1663
Cfr13I GGNCC 3 cut(s) 104, 680, 1381
Csp6I GTAC 7 cut(s) 218, 429, 469, 542, 880, 1097, 1445
CviAII CATG 9 cut(s) 170, 359, 539, 549, 659, 730, 1082, 1343, 1474
CviQI GTAC 7 cut(s) 218, 429, 469, 542, 880, 1097, 1445
DdeI CTNAG 7 cut(s) 527, 867, 1004, 1133, 1140, 1200, 1517
DpnI GATC 7 cut(s) 663, 826, 1063, 1123, 1179, 1282, 1628
DpnII GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
EaeI YGGCCR 2 cut(s) 734, 1050
Eam1104I CTCTTC 2 cut(s) 611, 1410
EarI CTCTTC 2 cut(s) 611, 1410
Eco130I CCWWGG 1 cut(s) 329
Eco47I GGWCC 3 cut(s) 104, 680, 1381
Eco57I CTGAAG 2 cut(s) 492, 1406
EcoO109I RGGNCCY 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 329
EcoT22I ATGCAT 1 cut(s) 1038
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 9 cut(s) 173, 362, 542, 552, 662, 733, 1085, 1346, 1477
FalI AAGNNNNNCTT 2 cut(s) 1434, 1466
FaqI GGGAC 1 cut(s) 1122
FatI CATG 9 cut(s) 169, 358, 538, 548, 658, 729, 1081, 1342, 1473
FauI CCCGC 1 cut(s) 480
FauNDI CATATG 3 cut(s) 455, 1038, 1113
FbaI TGATCA 1 cut(s) 1626
FblI GTMKAC 2 cut(s) 553, 1652
Fnu4HI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
FokI GGATG 4 cut(s) 689, 1166, 1564, 1573
Fsp4HI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
FspBI CTAG 1 cut(s) 557
GlaI GCGC 2 cut(s) 1472, 1662
GluI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
GsaI CCCAGC 4 cut(s) 30, 275, 291, 572
GsuI CTGGAG 3 cut(s) 760, 1014, 1457
HaeIII GGCC 2 cut(s) 736, 1052
HhaI GCGC 2 cut(s) 1473, 1663
Hin1II CATG 9 cut(s) 173, 362, 542, 552, 662, 733, 1085, 1346, 1477
Hin6I GCGC 2 cut(s) 1471, 1661
HinP1I GCGC 2 cut(s) 1471, 1661
HinfI GANTC 8 cut(s) 144, 497, 535, 586, 619, 655, 1649, 1676
HphI GGTGA 3 cut(s) 316, 1433, 1598
Hpy166II GTNNAC 3 cut(s) 554, 882, 1653
Hpy188I TCNGA 8 cut(s) 511, 666, 924, 964, 977, 1201, 1280, 1457
Hpy8I GTNNAC 3 cut(s) 554, 882, 1653
HpyAV CCTTC 7 cut(s) 478, 671, 975, 1208, 1381, 1529, 1542
HpyCH4III ACNGT 5 cut(s) 415, 433, 769, 1149, 1657
HpyCH4IV ACGT 1 cut(s) 849
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 863, 1520
HpyF3I CTNAG 7 cut(s) 527, 867, 1004, 1133, 1140, 1200, 1517
HpySE526I ACGT 1 cut(s) 849
Hsp92II CATG 9 cut(s) 173, 362, 542, 552, 662, 733, 1085, 1346, 1477
HspAI GCGC 2 cut(s) 1471, 1661
Ksp22I TGATCA 1 cut(s) 1626
Kzo9I GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
LmnI GCTCC 2 cut(s) 1018, 1277
Lsp1109I GCAGC 5 cut(s) 812, 1001, 1287, 1290, 1510
LweI GCATC 5 cut(s) 711, 765, 1144, 1230, 1501
MaeI CTAG 1 cut(s) 557
MaeII ACGT 1 cut(s) 849
MaeIII GTNAC 4 cut(s) 9, 458, 841, 1321
MalI GATC 7 cut(s) 663, 826, 1063, 1123, 1179, 1282, 1628
MbiI CCGCTC 1 cut(s) 475
MboI GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
MboII GAAGA 7 cut(s) 313, 385, 524, 628, 893, 1427, 1630
MfeI CAATTG 2 cut(s) 603, 738
MflI RGATCY 2 cut(s) 824, 1121
MhlI GDGCHC 1 cut(s) 914
MlsI TGGCCA 1 cut(s) 736
MluCI AATT 6 cut(s) 603, 738, 1054, 1285, 1409, 1580
MluNI TGGCCA 1 cut(s) 736
MlyI GAGTC 2 cut(s) 628, 1658
MmeI TCCRAC 3 cut(s) 730, 769, 1131
Mox20I TGGCCA 1 cut(s) 736
Mph1103I ATGCAT 1 cut(s) 1038
MroXI GAANNNNTTC 1 cut(s) 377
MscI TGGCCA 1 cut(s) 736
MseI TTAA 6 cut(s) 225, 417, 519, 636, 1368, 1499
MslI CAYNNNNRTG 1 cut(s) 363
Msp20I TGGCCA 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 992
MspR9I CCNGG 1 cut(s) 178
MunI CAATTG 2 cut(s) 603, 738
MvaI CCWGG 1 cut(s) 178
MwoI GCNNNNNNNGC 3 cut(s) 173, 863, 1520
NdeI CATATG 3 cut(s) 455, 1038, 1113
NdeII GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
NlaIII CATG 9 cut(s) 173, 362, 542, 552, 662, 733, 1085, 1346, 1477
NlaIV GGNNCC 3 cut(s) 105, 913, 1026
NmuCI GTSAC 2 cut(s) 9, 841
NsiI ATGCAT 1 cut(s) 1038
NspI RCATGY 2 cut(s) 173, 552
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 1036
PciI ACATGT 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 6 cut(s) 144, 497, 535, 586, 655, 1676
PkrI GCNGC 6 cut(s) 802, 991, 1051, 1302, 1305, 1525
PleI GAGTC 2 cut(s) 627, 1657
PpsI GAGTC 2 cut(s) 627, 1657
Ppu21I YACGTR 1 cut(s) 850
PpuMI RGGWCCY 1 cut(s) 104
PscI ACATGT 1 cut(s) 169
PshAI GACNNNNGTC 1 cut(s) 770
PsiI TTATAA 2 cut(s) 197, 1166
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 176
PspFI CCCAGC 4 cut(s) 26, 271, 287, 568
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 3 cut(s) 105, 913, 1026
PspPI GGNCC 3 cut(s) 104, 680, 1381
PspPPI RGGWCCY 1 cut(s) 104
PsrI GAACNNNNNNTAC 2 cut(s) 991, 1023
PsuI RGATCY 2 cut(s) 824, 1121
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 7 cut(s) 219, 430, 470, 543, 881, 1098, 1446
RsaNI GTAC 7 cut(s) 218, 429, 469, 542, 880, 1097, 1445
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 6 cut(s) 225, 417, 519, 636, 1368, 1499
SatI GCNGC 6 cut(s) 801, 990, 1050, 1301, 1304, 1524
Sau3AI GATC 7 cut(s) 661, 824, 1061, 1121, 1177, 1280, 1626
Sau96I GGNCC 3 cut(s) 104, 680, 1381
ScaI AGTACT 1 cut(s) 1446
SchI GAGTC 2 cut(s) 628, 1658
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 5 cut(s) 711, 765, 1144, 1230, 1501
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 3 cut(s) 104, 680, 1381
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 1506
SmoI CTYRAG 1 cut(s) 1506
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 6 cut(s) 603, 738, 1054, 1285, 1409, 1580
SsiI CCGC 2 cut(s) 473, 1049
SspI AATATT 1 cut(s) 237
SspMI CTAG 1 cut(s) 557
StyD4I CCNGG 1 cut(s) 176
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 5 cut(s) 415, 433, 769, 1149, 1657
TaiI ACGT 1 cut(s) 852
TaqI TCGA 6 cut(s) 649, 673, 904, 937, 1533, 1674
TasI AATT 6 cut(s) 603, 738, 1054, 1285, 1409, 1580
TatI WGTACW 3 cut(s) 428, 879, 1444
TauI GCSGC 1 cut(s) 1052
TfiI GAWTC 6 cut(s) 144, 497, 535, 586, 655, 1676
Tru1I TTAA 6 cut(s) 225, 417, 519, 636, 1368, 1499
Tru9I TTAA 6 cut(s) 225, 417, 519, 636, 1368, 1499
TscAI CASTG 2 cut(s) 604, 810
TseFI GTSAC 2 cut(s) 9, 841
TseI GCWGC 5 cut(s) 800, 989, 1300, 1303, 1523
Tsp45I GTSAC 2 cut(s) 9, 841
TspDTI ATGAA 6 cut(s) 527, 647, 1098, 1200, 1227, 1239
TspRI CASTG 2 cut(s) 604, 810
VpaK11BI GGWCC 3 cut(s) 104, 680, 1381
XapI RAATTY 2 cut(s) 1285, 1580
XceI RCATGY 2 cut(s) 173, 552
XmiI GTMKAC 2 cut(s) 553, 1652
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 1 cut(s) 557
ZrmI AGTACT 1 cut(s) 1446
Zsp2I ATGCAT 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.