pycom12g18770

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001181
Physical Location & Seq
Forward (+)
20877286 .. 20878449
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18770.1

Sequence Viewer

Length: 1164 bp
ATGGTCTTTAATGTGTCGCTTGGGTTTCAGAACTTACAGAGTGGTCGCAGTAATCCCAAAAACCAGAACTATGCTTTGTTGCTTGCGGACACTGTGGTTATCGGCAATGACAAGCCAGAGGTGGTGACAAGTAGAAGCTCTAAGAATCTTCAGGATGTGGCGTACTCCTTTAATGATGGAGATGAAGAGGAAGCTGAAGAACCAAAAAAACCAAAAGGTGAGGCTAATGGAAAGGAGGCCTTATTGTCTAAGACAACACTGAGGTCAGACAATCATGAGATTTCGAAGGAGGAACTCCGCAGGCAACATCAGGCAGAACTTGCCCGTCAGAAAAATGAAGAAACTGCCCGCAGACTTGCTGGTGGTGGATCTGGGTCGGGTGACAATCGTTCTGCAGCAAAGGCTTTAACAGATTTAGTTGCCTACAAGAATGTAAACGATGTCGTTCCTCCAAAAGGTCTGAATCAACTGATGATTCAGATTGACCAGAAGAACGAAGCTGTTCTGTTGCCTATTTATGGCAGTATGATCCCTTTTCATGTTGCCACCATAAGGACAGTTTCCAGTCAACAGGACACCAATCGGAACTGTTATATCAGAATCATGTTTAATGTCCCTGGGACTCCTTTCAGTCCTCATGATGCCAACTCACTGAAAAACCTTGGGTCTATATATTTAAAAGAAGTTTCATTTCGCTCCAAGGATCCAAGGCACATAAGTGAAGTGGTACAGGCGATTAAAGGGCTGCGAAGGCAAGTTGTATCCAGGGAGTCCTTGATAGCTGAGAAGGCGACACTGGTTACTCAGGAGAAACTCCAAATAGCGGGTAACAGATTTAAGCCAATAAGATTGTCTGACCTTTGGATTAGACCTGTTTTTGGTGGCCGTGGAAGAAAGATTCCTGGTACTCTTGAAGCTCATGCAAATGGGTTTCGCTATTCTACCACCAGGCAAGAGGAGCGCCTGGATGTCATGTTTGCAAATATTAAGCATGCCTTTTTCCAGCCAGCAGAGAATGAAATGATCGATCACCTGCACTTTCATCTGCACAATGACATCATGGTGGGCAACAAGAAAACGAAAAACGTGCAATTCTATGTTGAGGTGATGGACACAGTTCAGACCTTGGGAGGAAGCAGGAGGTCTGCCTATCGATCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

388

Amino Acids

43.49

Weight (kDa)

9.55

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT16 PF08644 159 - 272 2e-34 FACT complex subunit (SPT16/CDC68)
PH_SPT16 PF24824 282 - 384 2e-39 FACT complex subunit SPT16 PH-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1041
Acc36I ACCTGC 1 cut(s) 1041
AciI CCGC 4 cut(s) 86, 298, 349, 824
AclWI GGATC 5 cut(s) 376, 523, 698, 711, 1149
AcoI YGGCCR 1 cut(s) 883
AcuI CTGAAG 2 cut(s) 134, 216
AfaI GTAC 3 cut(s) 164, 729, 907
AfiI CCNNNNNNNGG 5 cut(s) 455, 518, 552, 823, 878
AgsI TTSAA 1 cut(s) 914
AjnI CCWGG 5 cut(s) 616, 764, 901, 947, 963
AleI CACNNNNGTG 1 cut(s) 717
AluBI AGCT 5 cut(s) 138, 194, 500, 782, 917
AluI AGCT 5 cut(s) 138, 194, 500, 782, 917
AlwI GGATC 5 cut(s) 376, 523, 698, 711, 1149
AoxI GGCC 2 cut(s) 237, 883
ApeKI GCWGC 2 cut(s) 395, 745
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 963
AsuHPI GGTGA 5 cut(s) 136, 230, 392, 1022, 1117
AsuII TTCGAA 1 cut(s) 284
BamHI GGATCC 1 cut(s) 703
BbvI GCAGC 2 cut(s) 407, 732
BccI CCATC 2 cut(s) 170, 1102
BceAI ACGGC 1 cut(s) 870
BcgI CGANNNNNNTGC 2 cut(s) 1069, 1103
BciT130I CCWGG 5 cut(s) 618, 766, 903, 949, 965
BciVI GTATCC 1 cut(s) 772
BfmI CTRYAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 964
BfuAI ACCTGC 1 cut(s) 1041
BfuI GTATCC 1 cut(s) 772
BisI GCNGC 2 cut(s) 396, 746
BlsI GCNGC 2 cut(s) 397, 747
Bme1390I CCNGG 5 cut(s) 618, 766, 903, 949, 965
BmiI GGNNCC 1 cut(s) 705
BmrFI CCNGG 5 cut(s) 618, 766, 903, 949, 965
BmsI GCATC 1 cut(s) 631
Bpu14I TTCGAA 1 cut(s) 284
Bsa29I ATCGAT 2 cut(s) 1026, 1153
BsaJI CCNNGG 8 cut(s) 616, 617, 661, 699, 707, 765, 886, 1125
Bsc4I CCNNNNNNNGG 5 cut(s) 455, 518, 552, 823, 878
Bse1I ACTGG 2 cut(s) 564, 801
Bse3DI GCAATG 1 cut(s) 112
BseBI CCWGG 5 cut(s) 618, 766, 903, 949, 965
BseCI ATCGAT 2 cut(s) 1026, 1153
BseDI CCNNGG 8 cut(s) 616, 617, 661, 699, 707, 765, 886, 1125
BseGI GGATG 2 cut(s) 160, 973
BseLI CCNNNNNNNGG 5 cut(s) 455, 518, 552, 823, 878
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 3 cut(s) 251, 774, 818
BseNI ACTGG 2 cut(s) 564, 801
BseRI GAGGAG 1 cut(s) 971
BseXI GCAGC 2 cut(s) 407, 732
BsgI GTGCAG 2 cut(s) 1019, 1031
BshFI GGCC 2 cut(s) 239, 885
BshVI ATCGAT 2 cut(s) 1026, 1153
BslFI GGGAC 2 cut(s) 599, 634
BslI CCNNNNNNNGG 5 cut(s) 455, 518, 552, 823, 878
BsmFI GGGAC 2 cut(s) 599, 634
BsnI GGCC 2 cut(s) 239, 885
Bsp119I TTCGAA 1 cut(s) 284
Bsp143I GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
BspACI CCGC 4 cut(s) 86, 298, 349, 824
BspANI GGCC 2 cut(s) 239, 885
BspCNI CTCAG 3 cut(s) 252, 775, 817
BspDI ATCGAT 2 cut(s) 1026, 1153
BspHI TCATGA 2 cut(s) 274, 637
BspLI GGNNCC 1 cut(s) 705
BspMAI CTGCAG 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 1041
BspPI GGATC 5 cut(s) 376, 523, 698, 711, 1149
BspT104I TTCGAA 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 112
BsrI ACTGG 2 cut(s) 564, 801
BssECI CCNNGG 8 cut(s) 616, 617, 661, 699, 707, 765, 886, 1125
BssMI GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
BssT1I CCWWGG 4 cut(s) 661, 699, 707, 1125
Bst2UI CCWGG 5 cut(s) 618, 766, 903, 949, 965
Bst4CI ACNGT 4 cut(s) 94, 559, 590, 1117
Bst6I CTCTTC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 5 cut(s) 84, 302, 349, 993, 1008
BstDEI CTNAG 5 cut(s) 141, 249, 260, 783, 804
BstDSI CCRYGG 1 cut(s) 886
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 2 cut(s) 160, 973
BstH2I RGCGCY 1 cut(s) 964
BstHHI GCGC 1 cut(s) 963
BstKTI GATC 6 cut(s) 371, 531, 706, 1026, 1030, 1157
BstMBI GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
BstMWI GCNNNNNNNGC 5 cut(s) 320, 401, 751, 788, 958
BstNI CCWGG 5 cut(s) 618, 766, 903, 949, 965
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 5 cut(s) 616, 764, 901, 947, 963
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 2 cut(s) 407, 732
BstX2I RGATCY 2 cut(s) 368, 703
BstYI RGATCY 2 cut(s) 368, 703
Bsu15I ATCGAT 2 cut(s) 1026, 1153
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 2 cut(s) 239, 885
BsuTUI ATCGAT 2 cut(s) 1026, 1153
BtgI CCRYGG 1 cut(s) 886
BtsCI GGATG 2 cut(s) 160, 973
BtsIMutI CAGTG 4 cut(s) 90, 257, 650, 794
BveI ACCTGC 1 cut(s) 1041
Cac8I GCNNGC 5 cut(s) 84, 302, 349, 993, 1008
CciI TCATGA 2 cut(s) 274, 637
CfoI GCGC 1 cut(s) 963
ClaI ATCGAT 2 cut(s) 1026, 1153
Csp6I GTAC 3 cut(s) 163, 728, 906
CviAII CATG 8 cut(s) 275, 539, 604, 638, 920, 973, 992, 1060
CviQI GTAC 3 cut(s) 163, 728, 906
DdeI CTNAG 5 cut(s) 141, 249, 260, 783, 804
DpnI GATC 6 cut(s) 370, 530, 705, 1025, 1029, 1156
DpnII GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
DraI TTTAAA 1 cut(s) 678
EaeI YGGCCR 1 cut(s) 883
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco130I CCWWGG 4 cut(s) 661, 699, 707, 1125
Eco147I AGGCCT 1 cut(s) 239
Eco57I CTGAAG 2 cut(s) 134, 216
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoRII CCWGG 5 cut(s) 616, 764, 901, 947, 963
EcoT14I CCWWGG 4 cut(s) 661, 699, 707, 1125
ErhI CCWWGG 4 cut(s) 661, 699, 707, 1125
FaeI CATG 8 cut(s) 278, 542, 607, 641, 923, 976, 995, 1063
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 980, 1012
FaqI GGGAC 2 cut(s) 599, 634
FatI CATG 8 cut(s) 274, 538, 603, 637, 919, 972, 991, 1059
FauI CCCGC 2 cut(s) 356, 817
Fnu4HI GCNGC 2 cut(s) 396, 746
FokI GGATG 2 cut(s) 167, 980
Fsp4HI GCNGC 2 cut(s) 396, 746
GlaI GCGC 1 cut(s) 962
GluI GCNGC 2 cut(s) 396, 746
HaeII RGCGCY 1 cut(s) 964
HaeIII GGCC 2 cut(s) 239, 885
HhaI GCGC 1 cut(s) 963
Hin1II CATG 8 cut(s) 278, 542, 607, 641, 923, 976, 995, 1063
Hin6I GCGC 1 cut(s) 961
HinP1I GCGC 1 cut(s) 961
HincII GTYRAC 1 cut(s) 569
HindII GTYRAC 1 cut(s) 569
HinfI GANTC 7 cut(s) 145, 463, 475, 600, 622, 770, 898
HphI GGTGA 5 cut(s) 136, 230, 392, 1022, 1117
Hpy166II GTNNAC 2 cut(s) 436, 569
Hpy188I TCNGA 9 cut(s) 30, 268, 330, 462, 480, 585, 599, 856, 1122
Hpy188III TCNNGA 6 cut(s) 152, 275, 638, 806, 911, 1158
Hpy8I GTNNAC 2 cut(s) 436, 569
HpyAV CCTTC 3 cut(s) 280, 744, 781
HpyCH4III ACNGT 4 cut(s) 94, 559, 590, 1117
HpyCH4IV ACGT 1 cut(s) 1086
HpyCH4V TGCA 6 cut(s) 395, 923, 980, 1036, 1048, 1090
HpyF10VI GCNNNNNNNGC 5 cut(s) 320, 401, 751, 788, 958
HpyF3I CTNAG 5 cut(s) 141, 249, 260, 783, 804
HpySE526I ACGT 1 cut(s) 1086
Hsp92II CATG 8 cut(s) 278, 542, 607, 641, 923, 976, 995, 1063
HspAI GCGC 1 cut(s) 961
Kzo9I GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
LmnI GCTCC 2 cut(s) 701, 958
Lsp1109I GCAGC 2 cut(s) 407, 732
LweI GCATC 1 cut(s) 631
MaeII ACGT 1 cut(s) 1086
MaeIII GTNAC 4 cut(s) 124, 380, 799, 827
MalI GATC 6 cut(s) 370, 530, 705, 1025, 1029, 1156
MboI GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
MboII GAAGA 6 cut(s) 140, 197, 209, 350, 502, 903
MflI RGATCY 2 cut(s) 368, 703
MluCI AATT 1 cut(s) 1091
MlyI GAGTC 2 cut(s) 616, 779
MroXI GAANNNNTTC 1 cut(s) 501
MseI TTAA 8 cut(s) 9, 171, 407, 609, 677, 738, 837, 987
MslI CAYNNNNRTG 3 cut(s) 717, 924, 1061
MspR9I CCNGG 5 cut(s) 618, 766, 903, 949, 965
MvaI CCWGG 5 cut(s) 618, 766, 903, 949, 965
MwoI GCNNNNNNNGC 5 cut(s) 320, 401, 751, 788, 958
NdeII GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
NlaIII CATG 8 cut(s) 278, 542, 607, 641, 923, 976, 995, 1063
NlaIV GGNNCC 1 cut(s) 705
NmuCI GTSAC 2 cut(s) 124, 380
NspI RCATGY 1 cut(s) 995
NspV TTCGAA 1 cut(s) 284
OliI CACNNNNGTG 1 cut(s) 717
PaeI GCATGC 1 cut(s) 995
PagI TCATGA 2 cut(s) 274, 637
PaqCI CACCTGC 1 cut(s) 1041
PasI CCCWGGG 1 cut(s) 617
PceI AGGCCT 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 5 cut(s) 145, 463, 475, 600, 898
PkrI GCNGC 2 cut(s) 397, 747
PleI GAGTC 2 cut(s) 616, 778
PpsI GAGTC 2 cut(s) 616, 778
Psp6I CCWGG 5 cut(s) 616, 764, 901, 947, 963
PspGI CCWGG 5 cut(s) 616, 764, 901, 947, 963
PspN4I GGNNCC 1 cut(s) 705
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 2 cut(s) 368, 703
RsaI GTAC 3 cut(s) 164, 729, 907
RsaNI GTAC 3 cut(s) 163, 728, 906
RseI CAYNNNNRTG 3 cut(s) 717, 924, 1061
SaqAI TTAA 8 cut(s) 9, 171, 407, 609, 677, 738, 837, 987
SatI GCNGC 2 cut(s) 396, 746
Sau3AI GATC 6 cut(s) 368, 528, 703, 1023, 1027, 1154
SchI GAGTC 2 cut(s) 616, 779
ScrFI CCNGG 5 cut(s) 618, 766, 903, 949, 965
SfaNI GCATC 1 cut(s) 631
SfcI CTRYAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 284
SmiMI CAYNNNNRTG 3 cut(s) 717, 924, 1061
SphI GCATGC 1 cut(s) 995
Sse9I AATT 1 cut(s) 1091
SseBI AGGCCT 1 cut(s) 239
SsiI CCGC 4 cut(s) 86, 298, 349, 824
SspI AATATT 1 cut(s) 985
StuI AGGCCT 1 cut(s) 239
StyD4I CCNGG 5 cut(s) 616, 764, 901, 947, 963
StyI CCWWGG 4 cut(s) 661, 699, 707, 1125
TaaI ACNGT 4 cut(s) 94, 559, 590, 1117
TaiI ACGT 1 cut(s) 1089
TaqI TCGA 3 cut(s) 284, 1026, 1153
TasI AATT 1 cut(s) 1091
TfiI GAWTC 5 cut(s) 145, 463, 475, 600, 898
Tru1I TTAA 8 cut(s) 9, 171, 407, 609, 677, 738, 837, 987
Tru9I TTAA 8 cut(s) 9, 171, 407, 609, 677, 738, 837, 987
TscAI CASTG 4 cut(s) 97, 264, 657, 801
TseFI GTSAC 2 cut(s) 124, 380
TseI GCWGC 2 cut(s) 395, 745
Tsp45I GTSAC 2 cut(s) 124, 380
TspDTI ATGAA 6 cut(s) 198, 351, 527, 678, 1031, 1032
TspRI CASTG 4 cut(s) 97, 264, 657, 801
XagI CCTNNNNNAGG 1 cut(s) 453
XceI RCATGY 1 cut(s) 995
XmnI GAANNNNTTC 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.