Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001181

Physical Location & Seq

Genomic Coordinates Reverse (-)

27937051 .. 27941517

Gene Structure 4467 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1188800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3216 bp

ATGGCTGAGCAACGAAAAGGTAATGTCAAACCTGCGAATGGGAAGCCATCAGGAACCGCAACAGCAGCGGCCAGTGCATATTCCATTGATCTCAATAACTTCAGCAAGCGGTTAAAGATGTTATATTCACATTGGAGGGAACATCGTAGTGATCTATGGGGTGAATCTGATGCACTTGCAATAGCAACTCCTCCAACTTCTGAGGATCTGCGGTATCTGAAATCCTCAGCAATGAATATCTGGTTGCTTGGTTATGAGTTCCCAGAAACTATCATGGTTTTTGCAAAGAAGCAGATCCATGTTTTGTGTAGCCAGAAAAAGGCCTCTCTCCTTGATGCTGTGATAAAGCCTGCAAAAGAGGCTGTTGGTGTTGAAGTGGTTATGCATGTAAAACTCAAAGGCCAGGATGGAACTGGACTGATGGATAGCATATTCCGAGCTGTAAATCCCCACTCGAGCTCCGATGCTCCTGTTGTCGGACACATAGCAAGAGAGGCTCCTGAAGGGAAACTTTTGGAGACTTGGAGTGAGAAGTTGAAGAATGCTAATTTTGAGTTGAGTGATGTGACCAATGGGTTCTCGGACTTGTTTTCCGTCAAAGACCCCGTTGAGCTTACATATGTGAAGAAAGCGGCATACTTGACTTCATCAGTGATGAAGACTTTTGTGGTACCTAAGCTTGAGAAGGTCATTGATGAGGAAAAGAAAATTTCCCATTCTTCATTGATGGATGACACGGAAAAGGTCATATTGGAGCCTGCAAGAATTAAGGTGAAGCTGACGGCAGCAAATGTTGATATTTGCTATCCTCCAATTTTTCAGAGTGGAGGGCAATTTGATCTGAAGCCAAGTGCTTCAAGCAATGATGAGAACCTTTGTTATGACTCTACTAGTGTGATTATATGTGCTGTCGGATCAAGGTACAAAAGCTACTGCTCCAATATTGCTCGAACATATCTGATTGATGCAAATTCTACGCAGAGCAAGGCTTATGAGGTTCTGCTTAAAGCTCAAGAAGCTGCAATCAGTAATTTAAAATCTGGGAAGAAGTTAAGTGCTGCATACCAAGCTGCACTCTCAGTAGTTGAAAAGGAGGCTCCAGAGCTGGCTGGAAACTTGACTAAAACAGCTGGAACTGGAATTGGCCTTGAGTTTCGTGAGTCGGGGCTTAATCTTAATGCTAAGAATGACCGAATTTTGAGACCAGGGATGGTTTTCAACGTTTCTCTGGGTTTCCAGAACGTGCAGGCACAGACAAAAGACCCTAAGACCCAGATATTTTCACTGTTAATAGCAGATACAGTTATTGTTGGTAAGGAGACCCCTGAAGTTTTGACTAATTCAAGCAAAGCTGTTAAGGATGTGGCGTACTCATTCAATGATGATGATGAAGAAGAAGAGCAGGCAAAACCTAGATCCAGTAGTAAAGGTGCCGGGAGTGCGATGAGTAAGGCCACACTTAGATCAGACAACCATGAAATGTCGAAAGAAGAGCTACGAAGGCAGCACCAAGCCGAACTTGCCCGCCAAAAGAATGAAGAAACTGCTAGAAGGCTTGCTGGTGGGGGTTCTGCAGCTAACGACAATCGTGGTGCTGGGAAGACAGTTGGTGATCTGATTGCATATAAGAATGTCAATGATCTGCCTGCTCCAAAAGAGTTGATGATTCAGGTTGACCAGAGGAATGAAGCTATCCTCTTGCCGGTTTATGGAAACATGATTCCTTTTCACATAGCCACTGTGAAGAGTGTTTCCAGCCAGCAGGATACTAACCGGAATTGCTACATCCGTATAATCTTTAATGTACCTGGTACTCCATTTAGCCCCCATGATGCAAACACCCTGAAGTTTCAAGGGTCAATTTTTTTAAAGGAAGTTTCATTCCGTTCCAAGGACCCGAGGCATATAAGTGAAGTAGTACAGCAAATTAAAACTCTTCGCAGGCAGGTTGCCTCAAGGGAATCTGAAAAAGCTGAAAGGGCAACTTTAGTTACGCAGGAGAAGCTGCAAATTTCAGGAGCCAAATTCAAGCCAAAAAGATTGCCAGATCTATGGATTCGCCCCGTTTTTGGGGGACGTGGAAGAAAGCTCACTGGATCACTGGAAGCCCACGCGAATGGATTCCGGTATTCGACTTCAAGGTCTGAAGAACGTGTCGATGTTATGTTTAGCAATATCAAACATGCATTTTTCCAGCCAGCTGAGAAGGAAATGATTACCCTGCTGCACTTTCATCTGCACGATCATATTATGGTAGGAAACAAAAAGACAAAGGACGTGCAATTTTATGCGGAGGTGATGGATGTAGTGCAGACACTGGGAGGTGGAAGAAGGTCAAACTATGATCCTGATGAGATCGAGGAAGAGCAACGTGAAAGGGAACGAAAGAACAAAATTAATATGGAGTTCCAGAACTTTGTGAACCGAGTAAATGATTCGTGGGGGCAACCTCCATTCAAATCACTTGATCTTGAGTTTGATCAGCCCCTTAGAGAGCTTGGCTTCCATGGAGTACCTCATAAAGCGTCAGCTTACATTGTCCCAACTTCAAGCTGTCTGGTTGAGCTGATAGAGACACCGTTTGTGGTAATTACTCTGAGCGAGATTGAAATAGTAAACCTTGAGAGAGTTGGTCTAGGGCAAAAGAACTTTGATCTGACTATTGTTTTCAAGGACTTCAAGCGAGATGTCTTCCGAATTGATTCTATTCCCTCGACATCTCTAGATGGAATTAAGGAGTGGTTAGACACCACTGATCTCAAGTATTATGAGAGTAGGTTGAATCTCAACTGGCGTCCTATCCTCAAAACCATTACTGACGACCCCGAGAAGTTCATAGAGGATGGTGGATGGGAATTTTTGAACATGGATATTAGTGATTCGGATTCCGACAATTCTCAGGAGTCTGACCATGGGTATGTGCCTTCAGATGTACAGTCGGATTCAGGTTCAGATGAGGAGGATGATGACAGCGAGTCATTGGTGGAGTCGGAAGATGATGAGGAAGAAGAATCCGGAGAAGACTCAGAAGAGGCTGAAGGAAAGACATGGGAAGAGCTGGAGAGGGAAGCAAGCCACGCGGACAGGGAAAAAGGGAATGGGTCGGACAGTGAAGAGGAGAGGGCAAGAAGGAAGGTTAAGGCTTTTGGAAAGGCTCGAGTTCCCGACAAGAGGAACATTGGTGGCAGCCTGCCCAAGAGGCCCAAATTTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1072

Amino Acids

119.71

Weight (kDa)

5.7

Isoelectric Point (pI)

45.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FACT-Spt16_Nlob	PF14826	29 - 192	5.7e-49	FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24	PF00557	208 - 437	6.2e-29	Metallopeptidase family M24
SPT16	PF08644	556 - 669	2.5e-37	FACT complex subunit (SPT16/CDC68)
PH_SPT16	PF24824	680 - 810	1.6e-55	FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle	PF08512	837 - 924	2.7e-23	Histone chaperone Rttp106-like, middle domain
SPT16_C	PF21091	963 - 1045	4.4e-16	FACT complex subunit SPT16, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10710 AT4G10710 AT4G10710
fragaria_vesca	FvH4_1g19730 FvH4_1g19730 FvH4_1g19730 FvH4_6g08280
malus_domestica	MD04G1188700.v1.1 MD04G1188800.v1.1 MD12G1201500.v1.1 MD12G1201600.v1.1
prunus_persica	Prupe.6G306600_v2.0.a1 Prupe.6G306700_v2.0.a1 Prupe.6G306700_v2.0.a1
pyrus_communis	pycom04g16700 pycom12g18760 pycom12g18770 pycom12g18790
rosa_chinensis	RchiOBHm_Chr2g0111311 RchiOBHm_Chr3g0458161 RchiOBHm_Chr3g0460191
rosa_laevigata	RLG00000017884 RLG00000025036 RLG00000025174
rosa_multiflora	Rmu_co8443511.1_g000001 Rmu_sc0000449.1_g000044 Rmu_sc0001363.1_g000004 Rmu_sc0003763.1_g000005 Rmu_sc0005033.1_g000004 Rmu_sc0019318.1_g000002
rosa_roxburghii	Rroxscaffold_2G00132510 Rroxscaffold_6G00419750 Rroxscaffold_6G00421250
rosa_rugosa	Rorug02G0172800 Rorug02G0172800 Rorug03G0025200 Rorug03G0040700 Rorug03G0040800 Rorug03G0040900 Rorug03G0041000 Rorug03G0041000 Rorug03G0041100
rosa_samantha	Rh2AG225800 Rh2BG237500 Rh2CG228800 Rh2DG233300 Rh3AG086000 Rh3AG098500 Rh3BG088700 Rh3BG101700 Rh3CG089300 Rh3CG103800 Rh3DG089700 Rh3DG103000
rosa_wichuraiana	Rw2G017430 Rw3G007300 Rw3G008330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2140
Acc36I	ACCTGC	2 cut(s)	40, 1936
Acc65I	GGTACC	1 cut(s)	670
AccB1I	GGYRCC	2 cut(s)	670, 1430
AccII	CGCG	2 cut(s)	2114, 3080
AccIII	TCCGGA	1 cut(s)	3014
AciI	CCGC	8 cut(s)	57, 68, 109, 211, 632, 1525, 2291, 3080
AclI	AACGTT	1 cut(s)	1221
AclWI	GGATC	6 cut(s)	213, 289, 922, 1410, 2104, 2339
AcoI	YGGCCR	1 cut(s)	69
AcsI	RAATTY	7 cut(s)	708, 970, 1194, 2010, 2024, 2855, 3206
AcuI	CTGAAG	8 cut(s)	85, 522, 863, 1347, 1865, 2166, 2910, 3057
AcyI	GRCGYC	1 cut(s)	2794
AfaI	GTAC	8 cut(s)	672, 923, 1370, 1806, 1813, 1920, 2514, 2934
AflIII	ACRYGT	1 cut(s)	2152
AhdI	GACNNNNNGTC	1 cut(s)	2974
AhlI	ACTAGT	1 cut(s)	890
AjiI	CACGTC	2 cut(s)	2078, 2278
AjnI	CCWGG	3 cut(s)	402, 1204, 1807
Alw21I	GWGCWC	1 cut(s)	461
Alw26I	GTCTC	4 cut(s)	512, 1195, 1313, 2567
AlwI	GGATC	6 cut(s)	213, 289, 922, 1410, 2104, 2339
AlwNI	CAGNNNCTG	1 cut(s)	2317
Ama87I	CYCGRG	4 cut(s)	454, 1897, 2825, 3156
Aor13HI	TCCGGA	1 cut(s)	3014
AoxI	GGCC	6 cut(s)	69, 321, 400, 1144, 1452, 3200
ApoI	RAATTY	7 cut(s)	708, 970, 1194, 2010, 2024, 2855, 3206
AseI	ATTAAT	1 cut(s)	2397
Asp700I	GAANNNNTTC	2 cut(s)	2120, 2701
Asp718I	GGTACC	1 cut(s)	670
AspS9I	GGNCC	2 cut(s)	1894, 3201
AsuC2I	CCSGG	1 cut(s)	1435
AsuHPI	GGTGA	4 cut(s)	173, 784, 1622, 2308
AvaI	CYCGRG	4 cut(s)	454, 1897, 2825, 3156
AvaII	GGWCC	1 cut(s)	1894
BanI	GGYRCC	2 cut(s)	670, 1430
BanII	GRGCYC	1 cut(s)	461
BbsI	GAAGAC	4 cut(s)	665, 1607, 2683, 3027
Bbv12I	GWGCWC	1 cut(s)	461
BbvCI	CCTCAGC	1 cut(s)	226
BccI	CCATC	9 cut(s)	55, 401, 415, 721, 1204, 2293, 2720, 2837, 2844
BceAI	ACGGC	1 cut(s)	798
BciT130I	CCWGG	3 cut(s)	404, 1206, 1809
BciVI	GTATCC	1 cut(s)	1759
BclI	TGATCA	1 cut(s)	2479
BcnI	CCSGG	1 cut(s)	1435
BcoDI	GTCTC	4 cut(s)	512, 1195, 1313, 2567
BcuI	ACTAGT	1 cut(s)	890
BfaI	CTAG	5 cut(s)	891, 1413, 1548, 2636, 2723
BfmI	CTRYAG	1 cut(s)	1572
BfuAI	ACCTGC	2 cut(s)	40, 1936
BfuI	GTATCC	1 cut(s)	1759
BglI	GCCNNNNNGGC	1 cut(s)	3199
BglII	AGATCT	1 cut(s)	2047
BlpI	GCTNAGC	1 cut(s)	6
Bme1390I	CCNGG	4 cut(s)	404, 1206, 1435, 1809
Bme18I	GGWCC	1 cut(s)	1894
BmeRI	GACNNNNNGTC	1 cut(s)	2974
BmeT110I	CYCGRG	4 cut(s)	454, 1897, 2825, 3156
BmgBI	CACGTC	2 cut(s)	2078, 2278
BmgT120I	GGNCC	2 cut(s)	1894, 3201
BmiI	GGNNCC	8 cut(s)	55, 498, 672, 756, 1098, 1432, 1896, 2020
BmrFI	CCNGG	4 cut(s)	404, 1206, 1435, 1809
BmrI	ACTGGG	1 cut(s)	2327
BmsI	GCATC	5 cut(s)	160, 325, 454, 955, 1822
BmuI	ACTGGG	1 cut(s)	2327
BpiI	GAAGAC	4 cut(s)	665, 1607, 2683, 3027
BpmI	CTGGAG	2 cut(s)	1083, 3080
Bpu10I	CCTNAGC	2 cut(s)	226, 675
Bpu1102I	GCTNAGC	1 cut(s)	6
BpuEI	CTTGAG	7 cut(s)	701, 996, 1169, 1939, 2492, 2642, 2744
BpuMI	CCSGG	1 cut(s)	1435
BsaHI	GRCGYC	1 cut(s)	2794
BsaI	GGTCTC	2 cut(s)	1195, 1313
BsaJI	CCNNGG	5 cut(s)	1205, 1890, 1898, 2506, 2911
BsaWI	WCCGGW	3 cut(s)	1773, 2124, 3014
BsaXI	ACNNNNNCTCC	2 cut(s)	443, 473
Bse118I	RCCGGY	1 cut(s)	1702
Bse1I	ACTGG	8 cut(s)	72, 418, 1141, 1419, 2098, 2106, 2322, 2795
Bse3DI	GCAATG	2 cut(s)	237, 868
BseAI	TCCGGA	1 cut(s)	3014
BseBI	CCWGG	3 cut(s)	404, 1206, 1809
BseDI	CCNNGG	5 cut(s)	1205, 1890, 1898, 2506, 2911
BseGI	GGATG	9 cut(s)	412, 736, 1215, 1366, 1785, 2308, 2848, 2855, 2968
BseMI	GCAATG	2 cut(s)	237, 868
BseMII	CTCAG	7 cut(s)	192, 240, 1092, 2193, 2588, 2912, 3039
BseNI	ACTGG	8 cut(s)	72, 418, 1141, 1419, 2098, 2106, 2322, 2795
BseRI	GAGGAG	3 cut(s)	180, 2972, 3131
BseYI	CCCAGC	1 cut(s)	1595
BsgI	GTGCAG	5 cut(s)	1056, 1265, 2210, 2222, 2330
Bsh1236I	CGCG	2 cut(s)	2114, 3080
BshFI	GGCC	6 cut(s)	71, 323, 402, 1146, 1454, 3202
BshNI	GGYRCC	2 cut(s)	670, 1430
BsiHKAI	GWGCWC	1 cut(s)	461
BsiHKCI	CYCGRG	4 cut(s)	454, 1897, 2825, 3156
BsiSI	CCGG	5 cut(s)	1434, 1703, 1774, 2125, 3015
BslFI	GGGAC	2 cut(s)	2088, 2525
BsmAI	GTCTC	4 cut(s)	512, 1195, 1313, 2567
BsmFI	GGGAC	2 cut(s)	2088, 2525
BsmI	GAATGC	1 cut(s)	547
BsnI	GGCC	6 cut(s)	71, 323, 402, 1146, 1454, 3202
Bso31I	GGTCTC	2 cut(s)	1195, 1313
BsoBI	CYCGRG	4 cut(s)	454, 1897, 2825, 3156
Bsp1286I	GDGCHC	1 cut(s)	461
Bsp13I	TCCGGA	1 cut(s)	3014
Bsp1407I	TGTACA	1 cut(s)	2932
Bsp1720I	GCTNAGC	1 cut(s)	6
Bsp19I	CCATGG	2 cut(s)	2506, 2911
BspACI	CCGC	8 cut(s)	57, 68, 109, 211, 632, 1525, 2291, 3080
BspANI	GGCC	6 cut(s)	71, 323, 402, 1146, 1454, 3202
BspCNI	CTCAG	7 cut(s)	193, 239, 1091, 2194, 2589, 2911, 3038
BspEI	TCCGGA	1 cut(s)	3014
BspFNI	CGCG	2 cut(s)	2114, 3080
BspLI	GGNNCC	8 cut(s)	55, 498, 672, 756, 1098, 1432, 1896, 2020
BspMAI	CTGCAG	1 cut(s)	1576
BspMI	ACCTGC	2 cut(s)	40, 1936
BspPI	GGATC	6 cut(s)	213, 289, 922, 1410, 2104, 2339
BspQI	GCTCTTC	4 cut(s)	1392, 1485, 2358, 3048
BspT107I	GGYRCC	2 cut(s)	670, 1430
BspTNI	GGTCTC	2 cut(s)	1195, 1313
BsrDI	GCAATG	2 cut(s)	237, 868
BsrFI	RCCGGY	1 cut(s)	1702
BsrGI	TGTACA	1 cut(s)	2932
BsrI	ACTGG	8 cut(s)	72, 418, 1141, 1419, 2098, 2106, 2322, 2795
BssAI	RCCGGY	1 cut(s)	1702
BssECI	CCNNGG	5 cut(s)	1205, 1890, 1898, 2506, 2911
BssNI	GRCGYC	1 cut(s)	2794
BssT1I	CCWWGG	3 cut(s)	1890, 2506, 2911
Bst2UI	CCWGG	3 cut(s)	404, 1206, 1809
Bst4CI	ACNGT	7 cut(s)	1287, 1303, 1606, 1741, 2580, 2937, 3110
Bst6I	CTCTTC	8 cut(s)	1392, 1485, 1739, 1941, 2358, 3024, 3048, 3108
BstACI	GRCGYC	1 cut(s)	2794
BstAUI	TGTACA	1 cut(s)	2932
BstDSI	CCRYGG	2 cut(s)	2506, 2911
BstF5I	GGATG	9 cut(s)	412, 736, 1215, 1366, 1785, 2308, 2848, 2855, 2968
BstFNI	CGCG	2 cut(s)	2114, 3080
BstMAI	GTCTC	4 cut(s)	512, 1195, 1313, 2567
BstNI	CCWGG	3 cut(s)	404, 1206, 1809
BstNSI	RCATGY	2 cut(s)	389, 2186
BstSCI	CCNGG	4 cut(s)	402, 1204, 1433, 1807
BstSFI	CTRYAG	1 cut(s)	1572
BstUI	CGCG	2 cut(s)	2114, 3080
BstV2I	GAAGAC	4 cut(s)	665, 1607, 2683, 3027
BstX2I	RGATCY	4 cut(s)	205, 294, 1415, 2047
BstXI	CCANNNNNNTGG	2 cut(s)	2052, 2117
BstYI	RGATCY	4 cut(s)	205, 294, 1415, 2047
BsuI	GTATCC	1 cut(s)	1759
BsuRI	GGCC	6 cut(s)	71, 323, 402, 1146, 1454, 3202
BtgI	CCRYGG	2 cut(s)	2506, 2911
BtgZI	GCGATG	1 cut(s)	1457
BtrI	CACGTC	2 cut(s)	2078, 2278
BtsCI	GGATG	9 cut(s)	412, 736, 1215, 1366, 1785, 2308, 2848, 2855, 2968
BtsIMutI	CAGTG	9 cut(s)	79, 657, 1283, 1737, 2091, 2099, 2315, 2751, 3115
BveI	ACCTGC	2 cut(s)	40, 1936
CaiI	CAGNNNCTG	1 cut(s)	2317
Cfr10I	RCCGGY	1 cut(s)	1702
Cfr13I	GGNCC	2 cut(s)	1894, 3201
CseI	GACGC	2 cut(s)	2514, 2783
CsiI	ACCWGGT	1 cut(s)	1807
Csp6I	GTAC	8 cut(s)	671, 922, 1369, 1805, 1812, 1919, 2513, 2933
CviQI	GTAC	8 cut(s)	671, 922, 1369, 1805, 1812, 1919, 2513, 2933
DraI	TTTAAA	2 cut(s)	1035, 1869
DrdI	GACNNNNNNGTC	1 cut(s)	2140
DriI	GACNNNNNGTC	1 cut(s)	2974
DseDI	GACNNNNNNGTC	1 cut(s)	2140
EaeI	YGGCCR	1 cut(s)	69
Eam1104I	CTCTTC	8 cut(s)	1392, 1485, 1739, 1941, 2358, 3024, 3048, 3108
Eam1105I	GACNNNNNGTC	1 cut(s)	2974
EarI	CTCTTC	8 cut(s)	1392, 1485, 1739, 1941, 2358, 3024, 3048, 3108
Ecl136II	GAGCTC	1 cut(s)	459
Eco130I	CCWWGG	3 cut(s)	1890, 2506, 2911
Eco147I	AGGCCT	1 cut(s)	323
Eco24I	GRGCYC	1 cut(s)	461
Eco31I	GGTCTC	2 cut(s)	1195, 1313
Eco47I	GGWCC	1 cut(s)	1894
Eco53kI	GAGCTC	1 cut(s)	459
Eco57I	CTGAAG	8 cut(s)	85, 522, 863, 1347, 1865, 2166, 2910, 3057
Eco88I	CYCGRG	4 cut(s)	454, 1897, 2825, 3156
EcoICRI	GAGCTC	1 cut(s)	459
EcoO109I	RGGNCCY	1 cut(s)	1894
EcoRII	CCWGG	3 cut(s)	402, 1204, 1807
EcoT14I	CCWWGG	3 cut(s)	1890, 2506, 2911
EcoT22I	ATGCAT	2 cut(s)	387, 2188
EcoT38I	GRGCYC	1 cut(s)	461
ErhI	CCWWGG	3 cut(s)	1890, 2506, 2911
FalI	AAGNNNNNCTT	6 cut(s)	495, 527, 1503, 1535, 1969, 2001
FaqI	GGGAC	2 cut(s)	2088, 2525
FauI	CCCGC	1 cut(s)	1532
FauNDI	CATATG	1 cut(s)	619
FbaI	TGATCA	1 cut(s)	2479
FokI	GGATG	9 cut(s)	419, 743, 1222, 1373, 1772, 2315, 2855, 2862, 2975
FriOI	GRGCYC	1 cut(s)	461
FspBI	CTAG	5 cut(s)	891, 1413, 1548, 2636, 2723
GsaI	CCCAGC	1 cut(s)	1599
GsuI	CTGGAG	2 cut(s)	1083, 3080
HaeIII	GGCC	6 cut(s)	71, 323, 402, 1146, 1454, 3202
HapII	CCGG	5 cut(s)	1434, 1703, 1774, 2125, 3015
HgaI	GACGC	2 cut(s)	2514, 2783
Hin1I	GRCGYC	1 cut(s)	2794
HincII	GTYRAC	1 cut(s)	1675
HindII	GTYRAC	1 cut(s)	1675
HindIII	AAGCTT	1 cut(s)	677
HpaII	CCGG	5 cut(s)	1434, 1703, 1774, 2125, 3015
HphI	GGTGA	4 cut(s)	173, 784, 1622, 2308
Hpy166II	GTNNAC	3 cut(s)	1675, 2422, 2617
Hpy8I	GTNNAC	3 cut(s)	1675, 2422, 2617
HpyCH4III	ACNGT	7 cut(s)	1287, 1303, 1606, 1741, 2580, 2937, 3110
HpyCH4IV	ACGT	6 cut(s)	1221, 1242, 2077, 2152, 2277, 2371
HpySE526I	ACGT	6 cut(s)	1221, 1242, 2077, 2152, 2277, 2371
Hsp92I	GRCGYC	1 cut(s)	2794
Kpn2I	TCCGGA	1 cut(s)	3014
KpnI	GGTACC	1 cut(s)	674
Ksp22I	TGATCA	1 cut(s)	2479
LguI	GCTCTTC	4 cut(s)	1392, 1485, 2358, 3048
LmnI	GCTCC	8 cut(s)	464, 472, 502, 754, 941, 1102, 1654, 2018
LweI	GCATC	5 cut(s)	160, 325, 454, 955, 1822
MabI	ACCWGGT	1 cut(s)	1807
MaeI	CTAG	5 cut(s)	891, 1413, 1548, 2636, 2723
MaeII	ACGT	6 cut(s)	1221, 1242, 2077, 2152, 2277, 2371
MaeIII	GTNAC	2 cut(s)	565, 1990
MflI	RGATCY	4 cut(s)	205, 294, 1415, 2047
MhlI	GDGCHC	1 cut(s)	461
MlyI	GAGTC	6 cut(s)	878, 1169, 2912, 2984, 2996, 3017
MmeI	TCCRAC	7 cut(s)	218, 457, 892, 2913, 2919, 2970, 3084
Mph1103I	ATGCAT	2 cut(s)	387, 2188
MroI	TCCGGA	1 cut(s)	3014
MroXI	GAANNNNTTC	2 cut(s)	2120, 2701
MslI	CAYNNNNRTG	4 cut(s)	147, 1908, 2115, 2916
MspA1I	CMGCKG	3 cut(s)	68, 1130, 2201
MspI	CCGG	5 cut(s)	1434, 1703, 1774, 2125, 3015
MspR9I	CCNGG	4 cut(s)	404, 1206, 1435, 1809
Mva1269I	GAATGC	1 cut(s)	547
MvaI	CCWGG	3 cut(s)	404, 1206, 1809
MvnI	CGCG	2 cut(s)	2114, 3080
NciI	CCSGG	1 cut(s)	1435
NcoI	CCATGG	2 cut(s)	2506, 2911
NdeI	CATATG	1 cut(s)	619
NlaIV	GGNNCC	8 cut(s)	55, 498, 672, 756, 1098, 1432, 1896, 2020
NmuCI	GTSAC	1 cut(s)	565
NsiI	ATGCAT	2 cut(s)	387, 2188
NspI	RCATGY	2 cut(s)	389, 2186
PaeR7I	CTCGAG	2 cut(s)	454, 3156
PceI	AGGCCT	1 cut(s)	323
PciSI	GCTCTTC	4 cut(s)	1392, 1485, 2358, 3048
PctI	GAATGC	1 cut(s)	547
PdmI	GAANNNNTTC	2 cut(s)	2120, 2701
PleI	GAGTC	6 cut(s)	878, 1168, 2911, 2983, 2995, 3017
PpsI	GAGTC	6 cut(s)	878, 1168, 2911, 2983, 2995, 3017
PpuMI	RGGWCCY	1 cut(s)	1894
PshBI	ATTAAT	1 cut(s)	2397
Psp124BI	GAGCTC	1 cut(s)	461
Psp1406I	AACGTT	1 cut(s)	1221
Psp5II	RGGWCCY	1 cut(s)	1894
Psp6I	CCWGG	3 cut(s)	402, 1204, 1807
PspFI	CCCAGC	1 cut(s)	1595
PspGI	CCWGG	3 cut(s)	402, 1204, 1807
PspN4I	GGNNCC	8 cut(s)	55, 498, 672, 756, 1098, 1432, 1896, 2020
PspPI	GGNCC	2 cut(s)	1894, 3201
PspPPI	RGGWCCY	1 cut(s)	1894
PspXI	VCTCGAGB	2 cut(s)	454, 3156
PstI	CTGCAG	1 cut(s)	1576
PstNI	CAGNNNCTG	1 cut(s)	2317
PsuI	RGATCY	4 cut(s)	205, 294, 1415, 2047
PvuII	CAGCTG	2 cut(s)	1130, 2201
RsaI	GTAC	8 cut(s)	672, 923, 1370, 1806, 1813, 1920, 2514, 2934
RsaNI	GTAC	8 cut(s)	671, 922, 1369, 1805, 1812, 1919, 2513, 2933
RseI	CAYNNNNRTG	4 cut(s)	147, 1908, 2115, 2916
SacI	GAGCTC	1 cut(s)	461
SapI	GCTCTTC	4 cut(s)	1392, 1485, 2358, 3048
Sau96I	GGNCC	2 cut(s)	1894, 3201
SchI	GAGTC	6 cut(s)	878, 1169, 2912, 2984, 2996, 3017
ScrFI	CCNGG	4 cut(s)	404, 1206, 1435, 1809
SduI	GDGCHC	1 cut(s)	461
SexAI	ACCWGGT	1 cut(s)	1807
SfaNI	GCATC	5 cut(s)	160, 325, 454, 955, 1822
SfcI	CTRYAG	1 cut(s)	1572
Sfr274I	CTCGAG	2 cut(s)	454, 3156
SinI	GGWCC	1 cut(s)	1894
SlaI	CTCGAG	2 cut(s)	454, 3156
SmiMI	CAYNNNNRTG	4 cut(s)	147, 1908, 2115, 2916
SmlI	CTYRAG	9 cut(s)	454, 680, 1011, 1148, 1954, 2471, 2621, 2759, 3156
SmoI	CTYRAG	9 cut(s)	454, 680, 1011, 1148, 1954, 2471, 2621, 2759, 3156
SpeI	ACTAGT	1 cut(s)	890
SseBI	AGGCCT	1 cut(s)	323
SsiI	CCGC	8 cut(s)	57, 68, 109, 211, 632, 1525, 2291, 3080
SspI	AATATT	1 cut(s)	943
SspMI	CTAG	5 cut(s)	891, 1413, 1548, 2636, 2723
SstI	GAGCTC	1 cut(s)	461
StuI	AGGCCT	1 cut(s)	323
StyD4I	CCNGG	4 cut(s)	402, 1204, 1433, 1807
StyI	CCWWGG	3 cut(s)	1890, 2506, 2911
TaaI	ACNGT	7 cut(s)	1287, 1303, 1606, 1741, 2580, 2937, 3110
TaiI	ACGT	6 cut(s)	1224, 1245, 2080, 2155, 2280, 2374
TaqI	TCGA	8 cut(s)	455, 949, 1484, 2132, 2157, 2358, 2714, 3157
TaqII	GACCGA	1 cut(s)	1206
TatI	WGTACW	2 cut(s)	1918, 2932
TauI	GCSGC	2 cut(s)	71, 635
TscAI	CASTG	9 cut(s)	79, 657, 1290, 1744, 2098, 2106, 2322, 2758, 3115
TseFI	GTSAC	1 cut(s)	565
Tsp45I	GTSAC	1 cut(s)	565
TspGWI	ACGGA	4 cut(s)	583, 752, 1778, 1874
TspRI	CASTG	9 cut(s)	79, 657, 1290, 1744, 2098, 2106, 2322, 2758, 3115
VpaK11BI	GGWCC	1 cut(s)	1894
VspI	ATTAAT	1 cut(s)	2397
XapI	RAATTY	7 cut(s)	708, 970, 1194, 2010, 2024, 2855, 3206
XbaI	TCTAGA	1 cut(s)	2722
XceI	RCATGY	2 cut(s)	389, 2186
XcmI	CCANNNNNNNNNTGG	1 cut(s)	410
XhoI	CTCGAG	2 cut(s)	454, 3156
XmnI	GAANNNNTTC	2 cut(s)	2120, 2701
XspI	CTAG	5 cut(s)	891, 1413, 1548, 2636, 2723
Zsp2I	ATGCAT	2 cut(s)	387, 2188

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.