MD12G1201500.v1.1

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001181
Physical Location & Seq
Forward (+)
28207188 .. 28211242
4055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1201500.v1.1.491

Sequence Viewer

Length: 3207 bp
ATGGCTGATCGTCGAAATGGAAATAGCCAGCCTGCTAATGGGAAGACTGCCGCTGGAGCAGGAAGTGCCTATAATATTGATTTGAAAGTATTCACTGAGAGGTTGAAGTTACTTTATTCTCATTGGAATGAACATCGATCTGATTTGTGGGGTTCTTCTGATGTTGTTGCAATAGCGACACCAAAAGCATCAGAAGATCTGCGGTACCTTAAATCTTCAGCATTGAATATTTGGCTGGTGGGTTATGAGTTTCCGGAGACCATTATGGTTTTCATGAAGAAGCAGATTCATTTCCTGTGCAGTACGAAGAAGGTGTCTTTGCTGGAAGTTGTAAAAAAGTCTGCCAAGGAAGCTGTGGGTGTTGATGTCGTGATGCATGAGAAGACAAGGAGTGATGATGGAAGTGGTCTGATGGAGGCAATTTTAGGTGCCATTCGTGCTCAGTCGAAGGCTGATGGCCATGACACAGCAGTTGTTGGACATATTGCAAGGGAGGCCCCTGAGGGAAACCTCTTGGAATCATGGTCCAAGAAGCTTGAGGATGCAAATTTTCAGTTGGGTGATGTAACAAATGGGTTGTCTGACCTGTTTGCTGTTAAGGACAATGAAGAGATTTTGAATATCAAGAGAGCTGCTGTCTTGTCTGCTAGCGTCATGACCGAAGTAGTTGTTAAAAAACTTGAGACTGTTATTGATGAGGAGAAGGAAGTCACCCATTCTTCTTTGATGGATGAGGCAGAGAAGGCTATAATGGAACCCTCTAAAGCTAAAGTGAAGCTCCGGGCTGATAATGTTGATATATGTTACCCCCCGATATTTCAGAGTGGAGGACAGTTTGATCTCAGACCCAGTGCTGCTAGCAATGATGAGTTGCTTTATTATGATTCTGCTAGTGCGATCATATGTGCAGTTGGGTCTCGCTACAAGAGTTACTGCTCAAATATTGCCAGGTCCTTTTTGATTGATGCTACTTCCAGTCAGAGCAAGGCATATGAAGTTCTCTTCAAGGCCCATAATGCAGCCATTAGTGAGTTGAAGCCTGGGAAAAAGGTCAGTGATGCTTATCAAGCTGCCCTTGCTGTGGTGGAGAAGGAGGCTCCTGAATTTGTATCAAATTTGACAAAATCGGCTGGGACTGGTATCGGCCTTGAGTTCCGTGAATCTGGGTTGAATATAAATGCAAAGAATGAGCGGTTGGTAAAAGCAGGCATGGTCTTTAATGTGTCGCTTGGGTTTCAGAACTTACAGAGTGGTCGCAGTAATCCCAAAAACCAGAACTATGCTTTGTTGCTTGCTGACACTGTGGTTATTGGCAATGACAAGCCAGAGGTGGTGACAAGTAGAAGCTCTAAGAATCTTCAGGATGTGGCGTACTCCTTTAATGATGGAGATGAAGAGGAAGCTGAAGAGCCAAAAAAACCAAAGGGTGAGGCTAATGCAAGGGAGGCCTTATTGTCTAAGACAACACTGAGGTCAGACAATCATGAGATTTCGAAGGAGGAACTCCGCAGGCAACATCAGGCAGAACTTGCCCGTCAGAAAAATGAAGAAACTGCCCGCAGACTTGCTGGTGGTGGATCTGGGTCGGGTGACAATCGTTCTGCAGCAAAGGGTTTAACGGATTTAGTTGCCTACAAGAATGTAAATGATGTCGTTCCTCCAAAAGGTCTGAATCAACTGATGATTCAGATTGACCAGAGGAACGAAGCTGTTCTGTTGCCTATTTATGGCAGTATGATCCCTTTTCATGTTGCCACCATAAGGACAGTTTCCAGTCAACAGGACACCAATCGGAACTGTTATATCAGAATCATGTTTAATGTCCCTGGGACTCCTTTCAGTCCTCATGATGCCAACTCACTGAAGAACCTTGGGTCTATATATTTAAAAGAAGTTTCATTTCGCTCCAAGGATCCAAGGCACATAAGTGAAGTGGTACAGGCGATTAAAGGGCTGCGAAGGCAAGTTGTATCCAGGGAGTCCTTGATAGCTGAGAAGGCGACACTGGTTACTCAAGAGAAACTCCAAATAGCAGGTAACAGATTTAAGCCGATAAGATTGTCTGACCTTTGGATTAGACCTGTTTTTGGTGGCCGTGGAAGAAAGATTCCTGGTACTCTTGAAGCTCATGCAAATGGGTTTCGTTATTCTACCACCAGGCAAGAGGAGCGCCTGGATGTCATGTTTGCAAATATTAAGCATGCCTTTTTCCAGCCAGCAGAGAATGAAATGATCACTCTCCTGCACTTTCATCTGCACAATGACATCATGGTGGGCAACAAGAAAACGAAAAACGTGCAATTCTATGTTGAGGTGATGGACACAGTTCAGACATTGGGAGGTAGCAGGAGGTCTGCCTATGATCCAGATGAGATTGAAGAAGAGCAGCGGGAGAGGGATAGGAAAAACAAAGTCAATATGAATTTTCAGAGCTTTGTGAATCGGGTTAATGAGCTTTGGGGGCAGTCACAGTTTAGGGACCTGGAGTTTGATCAACCTCTGAGAGAGCTTGGTTTCCACGGTGTACCTCATAAATCCTCGGCATTTATTGTCCCTACTTCAGCCTGCTTGGTTGAACTGATAGAAACTCCTTTCCTTGTTGTCAGTCTAAGTGAGATTGAGATTGTGAATCTGGAAAGAGTTGGCCTTGGGCAGAAGAATTTTGACATGACAATTGTATTCAAGGACTTCAAAAGGGACGTTCTCCGGATTGATTCAATCCCTTCAACAGCGCTTGATGGTATCAAGGAATGGCTCGACACAACTGACCTCAAGTATTATGAGAGCAGGCTGAATCTGAACTGGAAACAGATACTGAAGACAATCACCGATGACCCGCAGAGCTTCATTGAAGATGGAGGTTGGGAATTTTTGAATTTGGAAGCGTCTGATTCAGAGTCTGAACAGTCGGAGGAGTCAGACCAGGGTTATGAACCATCGGATGTAGAATCTGCTGAATCGGAAGAATCAGATGATGAGGATTCTGATAGTGAGTCGGTTGTGGAGTCTGAGGATGATGGAGAAGCAGAGTCAGATGAGGATGGGTTATCGTGGGATGAGCTGGAGAGGGAAGCAAGCAATGCAGATAGGGAGAAGGGGAACGAATATGACAGTGAGGAGGACAGGAAGAGAAGGAAGACGAAAGCTTTTGGGAAGTCTCGAGCCCCACCTCCTAATAGCATATCGAAGCGGACTAAGATGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1069

Amino Acids

119.31

Weight (kDa)

5.34

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FACT-Spt16_Nlob PF14826 26 - 191 6.5e-48 FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24 PF00557 208 - 437 2e-29 Metallopeptidase family M24
SPT16 PF08644 562 - 675 1.6e-33 FACT complex subunit (SPT16/CDC68)
PH_SPT16 PF24824 685 - 816 2.2e-54 FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle PF08512 840 - 928 2.5e-23 Histone chaperone Rttp106-like, middle domain
SPT16_C PF21091 968 - 1046 6.2e-14 FACT complex subunit SPT16, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2024
Acc65I GGTACC 1 cut(s) 204
AccB1I GGYRCC 2 cut(s) 204, 428
AccBSI CCGCTC 1 cut(s) 1192
AccIII TCCGGA 2 cut(s) 253, 2706
AciI CCGC 8 cut(s) 51, 202, 1192, 1507, 1558, 2389, 2837, 3190
AclWI GGATC 5 cut(s) 1585, 1732, 1907, 1920, 2357
AcoI YGGCCR 2 cut(s) 457, 2092
AcsI RAATTY 7 cut(s) 547, 1103, 1114, 2422, 2659, 2867, 2875
AcuI CTGAAG 6 cut(s) 201, 1343, 1425, 1883, 2544, 2837
AfaI GTAC 6 cut(s) 206, 304, 1373, 1938, 2116, 2526
AfeI AGCGCT 1 cut(s) 2733
AfiI CCNNNNNNNGG 6 cut(s) 38, 1081, 1664, 1727, 1761, 2087
AjnI CCWGG 9 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2481, 2922
AjuI GAANNNNNNNTTGG 2 cut(s) 1178, 1210
AleI CACNNNNGTG 1 cut(s) 1926
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 4 cut(s) 251, 677, 921, 3162
AlwI GGATC 5 cut(s) 1585, 1732, 1907, 1920, 2357
AlwNI CAGNNNCTG 2 cut(s) 2815, 2900
Ama87I CYCGRG 1 cut(s) 3159
Aor13HI TCCGGA 2 cut(s) 253, 2706
Aor51HI AGCGCT 1 cut(s) 2733
AoxI GGCC 7 cut(s) 457, 495, 1008, 1144, 1446, 2092, 2644
ApeKI GCWGC 7 cut(s) 632, 854, 1019, 1070, 1604, 1954, 2386
ApoI RAATTY 7 cut(s) 547, 1103, 1114, 2422, 2659, 2867, 2875
ArsI GACNNNNNNTTYG 6 cut(s) 299, 331, 563, 595, 2471, 2503
Asp700I GAANNNNTTC 2 cut(s) 89, 1710
Asp718I GGTACC 1 cut(s) 204
AspLEI GCGC 2 cut(s) 2172, 2734
AspS9I GGNCC 5 cut(s) 496, 525, 951, 1009, 2479
AsuC2I CCSGG 1 cut(s) 782
AsuHPI GGTGA 7 cut(s) 572, 703, 1345, 1439, 1601, 2326, 2818
AsuII TTCGAA 1 cut(s) 1493
AsuNHI GCTAGC 2 cut(s) 647, 857
AvaI CYCGRG 1 cut(s) 3159
AvaII GGWCC 3 cut(s) 525, 951, 2479
AxyI CCTNAGG 1 cut(s) 501
BalI TGGCCA 1 cut(s) 459
BamHI GGATCC 1 cut(s) 1912
BanI GGYRCC 2 cut(s) 204, 428
BanII GRGCYC 1 cut(s) 3166
BbsI GAAGAC 4 cut(s) 50, 389, 2825, 3143
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 7 cut(s) 619, 841, 1031, 1057, 1616, 1941, 2398
BceAI ACGGC 1 cut(s) 2079
BcgI CGANNNNNNTGC 2 cut(s) 2278, 2312
BciT130I CCWGG 9 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2483, 2924
BciVI GTATCC 1 cut(s) 1981
BclI TGATCA 2 cut(s) 2232, 2491
BcnI CCSGG 1 cut(s) 782
BcoDI GTCTC 4 cut(s) 251, 677, 921, 3162
BfaI CTAG 3 cut(s) 648, 858, 891
BfmI CTRYAG 1 cut(s) 1602
BfoI RGCGCY 2 cut(s) 2173, 2735
BfuAI ACCTGC 1 cut(s) 2024
BfuI GTATCC 1 cut(s) 1981
BglII AGATCT 1 cut(s) 196
BisI GCNGC 8 cut(s) 51, 633, 855, 1020, 1071, 1605, 1955, 2387
BlsI GCNGC 8 cut(s) 52, 634, 856, 1021, 1072, 1606, 1956, 2388
Bme18I GGWCC 3 cut(s) 525, 951, 2479
BmeT110I CYCGRG 1 cut(s) 3159
BmgT120I GGNCC 5 cut(s) 496, 525, 951, 1009, 2479
BmiI GGNNCC 7 cut(s) 206, 430, 498, 756, 1098, 1914, 2480
BmrI ACTGGG 1 cut(s) 843
BmsI GCATC 6 cut(s) 197, 363, 532, 955, 1048, 1840
BmtI GCTAGC 2 cut(s) 651, 861
BmuI ACTGGG 1 cut(s) 843
BpiI GAAGAC 4 cut(s) 50, 389, 2825, 3143
BpmI CTGGAG 3 cut(s) 75, 2504, 3083
Bpu14I TTCGAA 1 cut(s) 1493
BpuEI CTTGAG 5 cut(s) 557, 701, 1169, 1998, 2756
BpuMI CCSGG 1 cut(s) 782
Bsa29I ATCGAT 1 cut(s) 136
BsaBI GATNNNNATC 1 cut(s) 1062
BsaI GGTCTC 2 cut(s) 251, 921
BsaWI WCCGGW 2 cut(s) 253, 2706
Bsc4I CCNNNNNNNGG 6 cut(s) 38, 1081, 1664, 1727, 1761, 2087
Bse1I ACTGG 6 cut(s) 849, 975, 1141, 1773, 2010, 2807
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 3 cut(s) 868, 1321, 3085
Bse8I GATNNNNATC 1 cut(s) 1062
BseAI TCCGGA 2 cut(s) 253, 2706
BseBI CCWGG 9 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2483, 2924
BseCI ATCGAT 1 cut(s) 136
BseGI GGATG 8 cut(s) 547, 736, 1369, 2182, 2947, 3019, 3046, 3061
BseJI GATNNNNATC 1 cut(s) 1062
BseLI CCNNNNNNNGG 6 cut(s) 38, 1081, 1664, 1727, 1761, 2087
BseMI GCAATG 3 cut(s) 868, 1321, 3085
BseMII CTCAG 8 cut(s) 87, 455, 492, 856, 1460, 1983, 2492, 3000
BseNI ACTGG 6 cut(s) 849, 975, 1141, 1773, 2010, 2807
BseRI GAGGAG 4 cut(s) 713, 2180, 2927, 3131
BseXI GCAGC 7 cut(s) 619, 841, 1031, 1057, 1616, 1941, 2398
BseYI CCCAGC 1 cut(s) 1130
BsgI GTGCAG 4 cut(s) 319, 927, 2228, 2240
BshFI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
BshNI GGYRCC 2 cut(s) 204, 428
BshVI ATCGAT 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 3159
BsiSI CCGG 3 cut(s) 254, 781, 2707
BslFI GGGAC 6 cut(s) 1147, 1808, 1843, 2492, 2537, 2711
BslI CCNNNNNNNGG 6 cut(s) 38, 1081, 1664, 1727, 1761, 2087
BsmAI GTCTC 4 cut(s) 251, 677, 921, 3162
BsmFI GGGAC 6 cut(s) 1147, 1808, 1843, 2492, 2537, 2711
BsnI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
Bso31I GGTCTC 2 cut(s) 251, 921
BsoBI CYCGRG 1 cut(s) 3159
Bsp119I TTCGAA 1 cut(s) 1493
Bsp1286I GDGCHC 2 cut(s) 442, 3166
Bsp13I TCCGGA 2 cut(s) 253, 2706
BspACI CCGC 8 cut(s) 51, 202, 1192, 1507, 1558, 2389, 2837, 3190
BspANI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
BspCNI CTCAG 8 cut(s) 88, 454, 493, 855, 1461, 1984, 2493, 3001
BspDI ATCGAT 1 cut(s) 136
BspEI TCCGGA 2 cut(s) 253, 2706
BspHI TCATGA 4 cut(s) 273, 654, 1483, 1846
BspLI GGNNCC 7 cut(s) 206, 430, 498, 756, 1098, 1914, 2480
BspMAI CTGCAG 1 cut(s) 1606
BspMI ACCTGC 1 cut(s) 2024
BspOI GCTAGC 2 cut(s) 651, 861
BspPI GGATC 5 cut(s) 1585, 1732, 1907, 1920, 2357
BspQI GCTCTTC 2 cut(s) 1401, 2376
BspT104I TTCGAA 1 cut(s) 1493
BspT107I GGYRCC 2 cut(s) 204, 428
BspTNI GGTCTC 2 cut(s) 251, 921
BsrBI CCGCTC 1 cut(s) 1192
BsrDI GCAATG 3 cut(s) 868, 1321, 3085
BsrI ACTGG 6 cut(s) 849, 975, 1141, 1773, 2010, 2807
BssT1I CCWWGG 5 cut(s) 345, 1870, 1908, 1916, 2647
Bst2UI CCWGG 9 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2483, 2924
Bst6I CTCTTC 6 cut(s) 603, 1007, 1389, 1401, 2376, 3122
BstAPI GCANNNNNTGC 3 cut(s) 65, 1529, 3080
BstBI TTCGAA 1 cut(s) 1493
BstDSI CCRYGG 2 cut(s) 2095, 2518
BstENI CCTNNNNNAGG 1 cut(s) 1662
BstF5I GGATG 8 cut(s) 547, 736, 1369, 2182, 2947, 3019, 3046, 3061
BstH2I RGCGCY 2 cut(s) 2173, 2735
BstHHI GCGC 2 cut(s) 2172, 2734
BstMAI GTCTC 4 cut(s) 251, 677, 921, 3162
BstNI CCWGG 9 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2483, 2924
BstNSI RCATGY 1 cut(s) 2204
BstSFI CTRYAG 1 cut(s) 1602
BstV1I GCAGC 7 cut(s) 619, 841, 1031, 1057, 1616, 1941, 2398
BstV2I GAAGAC 4 cut(s) 50, 389, 2825, 3143
BstX2I RGATCY 3 cut(s) 196, 1577, 1912
BstYI RGATCY 3 cut(s) 196, 1577, 1912
Bsu15I ATCGAT 1 cut(s) 136
Bsu36I CCTNAGG 1 cut(s) 501
BsuI GTATCC 1 cut(s) 1981
BsuRI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
BsuTUI ATCGAT 1 cut(s) 136
BtgI CCRYGG 2 cut(s) 2095, 2518
BtsCI GGATG 8 cut(s) 547, 736, 1369, 2182, 2947, 3019, 3046, 3061
BtsIMutI CAGTG 8 cut(s) 93, 856, 1060, 1299, 1466, 1859, 2003, 3118
BveI ACCTGC 1 cut(s) 2024
CaiI CAGNNNCTG 2 cut(s) 2815, 2900
CciI TCATGA 4 cut(s) 273, 654, 1483, 1846
CfoI GCGC 2 cut(s) 2172, 2734
Cfr13I GGNCC 5 cut(s) 496, 525, 951, 1009, 2479
ClaI ATCGAT 1 cut(s) 136
CseI GACGC 2 cut(s) 640, 2874
Csp6I GTAC 6 cut(s) 205, 303, 1372, 1937, 2115, 2525
CviQI GTAC 6 cut(s) 205, 303, 1372, 1937, 2115, 2525
DraI TTTAAA 1 cut(s) 1887
EaeI YGGCCR 2 cut(s) 457, 2092
Eam1104I CTCTTC 6 cut(s) 603, 1007, 1389, 1401, 2376, 3122
EarI CTCTTC 6 cut(s) 603, 1007, 1389, 1401, 2376, 3122
Eco130I CCWWGG 5 cut(s) 345, 1870, 1908, 1916, 2647
Eco147I AGGCCT 1 cut(s) 1448
Eco24I GRGCYC 1 cut(s) 3166
Eco31I GGTCTC 2 cut(s) 251, 921
Eco47I GGWCC 3 cut(s) 525, 951, 2479
Eco47III AGCGCT 1 cut(s) 2733
Eco57I CTGAAG 6 cut(s) 201, 1343, 1425, 1883, 2544, 2837
Eco81I CCTNAGG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 3159
EcoNI CCTNNNNNAGG 1 cut(s) 1662
EcoO109I RGGNCCY 3 cut(s) 496, 951, 2479
EcoRII CCWGG 9 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2481, 2922
EcoT14I CCWWGG 5 cut(s) 345, 1870, 1908, 1916, 2647
EcoT22I ATGCAT 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 3166
ErhI CCWWGG 5 cut(s) 345, 1870, 1908, 1916, 2647
FalI AAGNNNNNCTT 4 cut(s) 1059, 1091, 2189, 2221
FaqI GGGAC 6 cut(s) 1147, 1808, 1843, 2492, 2537, 2711
FauI CCCGC 3 cut(s) 1565, 2382, 2844
FauNDI CATATG 2 cut(s) 902, 991
FbaI TGATCA 2 cut(s) 2232, 2491
Fnu4HI GCNGC 8 cut(s) 51, 633, 855, 1020, 1071, 1605, 1955, 2387
FokI GGATG 8 cut(s) 554, 743, 1376, 2189, 2954, 3026, 3053, 3068
FriOI GRGCYC 1 cut(s) 3166
Fsp4HI GCNGC 8 cut(s) 51, 633, 855, 1020, 1071, 1605, 1955, 2387
FspBI CTAG 3 cut(s) 648, 858, 891
GlaI GCGC 2 cut(s) 2171, 2733
GluI GCNGC 8 cut(s) 51, 633, 855, 1020, 1071, 1605, 1955, 2387
GsaI CCCAGC 1 cut(s) 1134
GsuI CTGGAG 3 cut(s) 75, 2504, 3083
HaeII RGCGCY 2 cut(s) 2173, 2735
HaeIII GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
HapII CCGG 3 cut(s) 254, 781, 2707
HgaI GACGC 2 cut(s) 640, 2874
HhaI GCGC 2 cut(s) 2172, 2734
Hin6I GCGC 2 cut(s) 2170, 2732
HinP1I GCGC 2 cut(s) 2170, 2732
HincII GTYRAC 1 cut(s) 1778
HindII GTYRAC 1 cut(s) 1778
HindIII AAGCTT 2 cut(s) 533, 3144
HpaII CCGG 3 cut(s) 254, 781, 2707
HphI GGTGA 7 cut(s) 572, 703, 1345, 1439, 1601, 2326, 2818
Hpy166II GTNNAC 2 cut(s) 1778, 2525
Hpy8I GTNNAC 2 cut(s) 1778, 2525
Hpy99I CGWCG 1 cut(s) 15
HpyCH4IV ACGT 2 cut(s) 2295, 2700
HpySE526I ACGT 2 cut(s) 2295, 2700
HspAI GCGC 2 cut(s) 2170, 2732
Kpn2I TCCGGA 2 cut(s) 253, 2706
KpnI GGTACC 1 cut(s) 208
Ksp22I TGATCA 2 cut(s) 2232, 2491
LguI GCTCTTC 2 cut(s) 1401, 2376
LmnI GCTCC 5 cut(s) 56, 783, 1102, 1910, 2167
Lsp1109I GCAGC 7 cut(s) 619, 841, 1031, 1057, 1616, 1941, 2398
LweI GCATC 6 cut(s) 197, 363, 532, 955, 1048, 1840
MaeI CTAG 3 cut(s) 648, 858, 891
MaeII ACGT 2 cut(s) 2295, 2700
MbiI CCGCTC 1 cut(s) 1192
MfeI CAATTG 1 cut(s) 2673
MflI RGATCY 3 cut(s) 196, 1577, 1912
MhlI GDGCHC 2 cut(s) 442, 3166
MlsI TGGCCA 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 459
MlyI GAGTC 7 cut(s) 1825, 1988, 2906, 2924, 3002, 3014, 3038
MmeI TCCRAC 2 cut(s) 457, 2889
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 1 cut(s) 378
MroI TCCGGA 2 cut(s) 253, 2706
MroXI GAANNNNTTC 2 cut(s) 89, 1710
MscI TGGCCA 1 cut(s) 459
MslI CAYNNNNRTG 4 cut(s) 126, 1926, 2133, 2270
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 2 cut(s) 53, 2389
MspI CCGG 3 cut(s) 254, 781, 2707
MunI CAATTG 1 cut(s) 2673
MvaI CCWGG 9 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2483, 2924
NciI CCSGG 1 cut(s) 782
NdeI CATATG 2 cut(s) 902, 991
NheI GCTAGC 2 cut(s) 647, 857
NlaIV GGNNCC 7 cut(s) 206, 430, 498, 756, 1098, 1914, 2480
NmeAIII GCCGAG 1 cut(s) 2519
NmuCI GTSAC 4 cut(s) 709, 1333, 1589, 2466
NsiI ATGCAT 1 cut(s) 378
NspI RCATGY 1 cut(s) 2204
NspV TTCGAA 1 cut(s) 1493
OliI CACNNNNGTG 1 cut(s) 1926
PaeI GCATGC 1 cut(s) 2204
PaeR7I CTCGAG 1 cut(s) 3159
PagI TCATGA 4 cut(s) 273, 654, 1483, 1846
PasI CCCWGGG 1 cut(s) 1826
PceI AGGCCT 1 cut(s) 1448
PciSI GCTCTTC 2 cut(s) 1401, 2376
PdmI GAANNNNTTC 2 cut(s) 89, 1710
PkrI GCNGC 8 cut(s) 52, 634, 856, 1021, 1072, 1606, 1956, 2388
PleI GAGTC 7 cut(s) 1825, 1987, 2905, 2923, 3001, 3013, 3037
PpsI GAGTC 7 cut(s) 1825, 1987, 2905, 2923, 3001, 3013, 3037
PpuMI RGGWCCY 2 cut(s) 951, 2479
Psp5II RGGWCCY 2 cut(s) 951, 2479
Psp6I CCWGG 9 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2481, 2922
PspFI CCCAGC 1 cut(s) 1130
PspGI CCWGG 9 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2481, 2922
PspN4I GGNNCC 7 cut(s) 206, 430, 498, 756, 1098, 1914, 2480
PspPI GGNCC 5 cut(s) 496, 525, 951, 1009, 2479
PspPPI RGGWCCY 2 cut(s) 951, 2479
PstI CTGCAG 1 cut(s) 1606
PstNI CAGNNNCTG 2 cut(s) 2815, 2900
PsuI RGATCY 3 cut(s) 196, 1577, 1912
RsaI GTAC 6 cut(s) 206, 304, 1373, 1938, 2116, 2526
RsaNI GTAC 6 cut(s) 205, 303, 1372, 1937, 2115, 2525
RseI CAYNNNNRTG 4 cut(s) 126, 1926, 2133, 2270
SapI GCTCTTC 2 cut(s) 1401, 2376
SatI GCNGC 8 cut(s) 51, 633, 855, 1020, 1071, 1605, 1955, 2387
Sau96I GGNCC 5 cut(s) 496, 525, 951, 1009, 2479
SchI GAGTC 7 cut(s) 1825, 1988, 2906, 2924, 3002, 3014, 3038
SduI GDGCHC 2 cut(s) 442, 3166
SfaNI GCATC 6 cut(s) 197, 363, 532, 955, 1048, 1840
SfcI CTRYAG 1 cut(s) 1602
Sfr274I CTCGAG 1 cut(s) 3159
SfuI TTCGAA 1 cut(s) 1493
SinI GGWCC 3 cut(s) 525, 951, 2479
SlaI CTCGAG 1 cut(s) 3159
SmiMI CAYNNNNRTG 4 cut(s) 126, 1926, 2133, 2270
SmlI CTYRAG 6 cut(s) 536, 680, 1148, 2013, 2771, 3159
SmoI CTYRAG 6 cut(s) 536, 680, 1148, 2013, 2771, 3159
SphI GCATGC 1 cut(s) 2204
SseBI AGGCCT 1 cut(s) 1448
SsiI CCGC 8 cut(s) 51, 202, 1192, 1507, 1558, 2389, 2837, 3190
SspI AATATT 4 cut(s) 76, 229, 943, 2194
SspMI CTAG 3 cut(s) 648, 858, 891
StuI AGGCCT 1 cut(s) 1448
StyI CCWWGG 5 cut(s) 345, 1870, 1908, 1916, 2647
TaiI ACGT 2 cut(s) 2298, 2703
TaqI TCGA 7 cut(s) 13, 136, 446, 1493, 2757, 3160, 3185
TaqII GACCGA 1 cut(s) 674
TauI GCSGC 1 cut(s) 53
TscAI CASTG 8 cut(s) 100, 856, 1060, 1306, 1473, 1866, 2010, 3118
TseFI GTSAC 4 cut(s) 709, 1333, 1589, 2466
TseI GCWGC 7 cut(s) 632, 854, 1019, 1070, 1604, 1954, 2386
Tsp45I GTSAC 4 cut(s) 709, 1333, 1589, 2466
TspGWI ACGGA 2 cut(s) 1145, 1634
TspRI CASTG 8 cut(s) 100, 856, 1060, 1306, 1473, 1866, 2010, 3118
VpaK11BI GGWCC 3 cut(s) 525, 951, 2479
XagI CCTNNNNNAGG 1 cut(s) 1662
XapI RAATTY 7 cut(s) 547, 1103, 1114, 2422, 2659, 2867, 2875
XceI RCATGY 1 cut(s) 2204
XcmI CCANNNNNNNNNTGG 2 cut(s) 35, 352
XhoI CTCGAG 1 cut(s) 3159
XmnI GAANNNNTTC 2 cut(s) 89, 1710
XspI CTAG 3 cut(s) 648, 858, 891
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.