Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0001181

Physical Location & Seq

Genomic Coordinates Reverse (-)

23207327 .. 23211858

Gene Structure 4532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG233300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3243 bp

ATGGCTGATCATCGGAAAGGTAATGTGAAACCGGCGAATGGGAAGGCGTCAGGAACCACTGCAGGGAGCTCCAGTACGTATGTGATTGATGTCAATAACTTCAGCAAGCGGCTAAAGCTGTTGTATTCGCATTGGAATGAACACCACAGTGGTCTATGGGGTCACTCTGATGCACTTGCTATTGCTACTCCTCCGACTTCAGATGATCTGCGGTACCTGAAATCGTCGGCGCTGAATATCTGGCTGGTCGGGTTTGAGTTCCCGGATACTATCATGGTTTTCACAAAGAAGCAGATCCATGTTTTGTGTAGCCAGAAGAAAGCATCTCTGTTGGAAATTGTGAAGAAGCCTGCAAAAGAGGCTGTTGGTGTTGAAGTTGTGATGCATGTGAAGCTCAAGAGTCAGGATGGGACGGGGCTGATGGATACCATTTTTCAAGCTGTGAAGGCCCAATCGAGCTCCAGTAGTAGTTCTAATCCTGTTGTTGGACACATAGCAAGAGAGGCTCCGGAAGGGAAGCTTTTGGAGACTTGGATGGACAAGTTGAATAGTGCTAATTTCGAGTTGACTGATGTAACAAATGGGTTCTCAGACTTATTTGCTGTCAAAGACTCCGTGGAGCTTACGAATGTGAAGAAAGCTGCATTCTTGACTTCGTCGGTGATGAGGAGTTTTGTGGTACCTAAGCTTGAAAAGGTCATTGATGAGGAAAAGAAAATTTCTCATTCTTCACTGATGGATGAAACGGAAAAGACCATAGTGGAACCTGCAAGAATTAAGGTCAAGCTGAAGGCAGACAATGTTGATATCTGTTATCCTCCAATTTTTCAGAGTGGCGGAGGGTTTGACCTGAAACCAAGTGCTTCAAGCAACGATGAGAACCTTTGTTACGACTCTACCAGTGTAATTATATGTGCTGTTGGATCGCGGTACAACAGCTATTGCTCAAATGTTGCTCGGACCTTTCTGATTGATGCAAATTCTACACAGAGCAAAGCTTATGAGGTTCTGCTTAAAGCTCAAGAAGCTGCAATTAGTAAATTAAAAGCTGGCAACAAGTTAAGTGCTGCATACCAAGCTGCGATCTCAGTAGTTGAAAAGGAGGCTCCAGAGTTGGCTGCAAACTTGACTAAAACAGCAGGAACTGGAATTGGCCTTGAGTTTCGTGAGTCCGGTCTTAATCTGAATGCCAAGAATGATCGGATTTTCAAACCAGGCATGGTTTTCAATGTGTCTCTTGGTTTTCAGAACTTGCAGGCACAAACAAAAAACCCAAAGACCCAGATATTTTCACTGTTATTAGCGGATACAGTTATTGTTGGTAAAGAGAGCCCTGAAATTTTGACTAATGTAAGCTCCAAAGCTGTGAAGGATGTGGCTTACTCATTCAATGATGATGACGATGATGAAGATGAGAGGACAAAAATCAAAGCCAGCAGTAAAGGTGCTGGGAGCACCAAGAGTAAGGCCACACTCAGGTCAGACAACCATGAAATGTCAAAGGAAGAACTACGGAGGCAGCATCAAGCGGAACTTGCCCGCCAAAAGAATGAGGAAACTGCTAGGAGGCTTGCCGGTGGGGATTCTGCGGCAACAAACAATCGTGGTGCTGGGAAGACAATTGGTGATCTGATTGCATATAAGAATGTCAATGATATCTCTCCTCCAAGAGAGCTAATGATTCAGGTTGATCAGAAAAATGAAGCCATCCTCTTGCCAATTTATGGAAACATGGTCCCTTTCCATGTAGCCACCGTGAAGAGTGTTTCCAGCCATCAGGACACTACCCAGGACAGTAACCGTAATTGCTACATCCGTATAATCTTTAATGTACCTGGCACCCCATTTAGTCCTCATGATGCAAACTCCCTCAAGTTTCAAGGATCAATTTATTTGAAGGAAGTTTCATTCCGCTCTAAAGACCAAAGGCATATAAGTGAAGCAGTACAGCTTATAAAAACCCTTCGCCGCCAGGTTGCCTCAAGGGAGTCTGAAAGAGCTGAGAGGGCAACTTTAGTTACGCAGGAGAAGCTGCAACTTGCAGGAGCCAAATTCAAGCCAAAAAGATTGCCTGATCTAGAGATTCGTCCCAGTTTTGGTGGTCGTGCAAGAAAGCTCACAGGATCACTGGAAGCCCACGCAAATGGATTACGGTATTCTACTTCTAGGGCTGATCAACGTGTGGACGTTATGTTTAGCAATATCAAACATGCATTTTTCCAGCCAGCAGAGAGGGAAATGATTACCTTGTTGCACTTTCATCTGCACAATCACATTATGGTGGGTAACAAAAAGACCAAGGATGTACAATTTTATACGGAAGTGATGGATGTAGTCCAGACACTGGGTGGTGGAAAGAGGTCTGCCTATGATCCTGATGAGATTGAGGAAGAGCATCGTGAGAGACAACGAAAGAACAAAATTAACATGGAGTTCCAGAACTTCGTGAACCGAGTGAATGATATGTGGGGGCAACCTGAATTCAAATCTCTTGACCTCGAGTTTGATCAGCCTCTTAGAGAGCTTGGCTTCAATGGAGTACCCCATAAATCCTCGGCTTTCATTGTCCCTACTTCAAGCTGTCTGGTGGAGCTGATAGAGACACCGTTTGTGGTAATTACTCTAAGTGAGATTGAAATCGTGAACCTTGAGAGAGTTGGTCTTGGGCAAAAGAACTTTGATTTGACTATTGTATTCAAGGACTTCAAGCGGGATGTATTTAGAATTGATTCCATCCCGTCCACATCACTAGATGGCATCAAGGAGTGGTTAGACACAACTGATCTCAAGTATTATGAGAGCAGGTTGAATCTCAATTGGCGACCTATACTGAAAACCATTACTGATGACCCTGAAAAGTTCATAGAAGACGGTGGATGGGAATTTTTGAACCTGGAGGTCAGTGATTCTGATTCAGACAATTCTCAGGAGTCAGACCAAGGGTATGTGCCTTCGGATATTCAGTCAGAATCAGGTTCAGAAGATGAGGATGATGAGAGCGAGTCGTTGGTGGAGTCCGAGGATGATGAGGAAGAGGAGTCTGGAGAAGACTCAGAAGAGGAAGAAGGAAAGACATGGGAAGAGTTAGAGAGGGAAGCGAGCCATGCAGACAGGGAAAAAGGAAATGACTCCGACAGTGAAGAGGAGAGGGCAAGAAGGAAGGTGAAGTCTTTTGGAAAAGCTCGGGCGCCAGACAAGAGAAATCATGGTGGAAGCCTTCCTAAGAGGCCCAAATTTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1080

Amino Acids

120.61

Weight (kDa)

5.77

Isoelectric Point (pI)

42.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FACT-Spt16_Nlob	PF14826	29 - 195	9.6e-49	FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24	PF00557	211 - 440	6.3e-29	Metallopeptidase family M24
SPT16	PF08644	561 - 678	1.5e-35	FACT complex subunit (SPT16/CDC68)
PH_SPT16	PF24824	689 - 819	3.1e-54	FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle	PF08512	846 - 933	1.7e-23	Histone chaperone Rttp106-like, middle domain
SPT16_C	PF21091	972 - 1054	1.1e-16	FACT complex subunit SPT16, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10710 AT4G10710 AT4G10710
fragaria_vesca	FvH4_1g19730 FvH4_1g19730 FvH4_1g19730 FvH4_6g08280
malus_domestica	MD04G1188700.v1.1 MD04G1188800.v1.1 MD12G1201500.v1.1 MD12G1201600.v1.1
prunus_persica	Prupe.6G306600_v2.0.a1 Prupe.6G306700_v2.0.a1 Prupe.6G306700_v2.0.a1
pyrus_communis	pycom04g16700 pycom12g18760 pycom12g18770 pycom12g18790
rosa_chinensis	RchiOBHm_Chr2g0111311 RchiOBHm_Chr3g0458161 RchiOBHm_Chr3g0460191
rosa_laevigata	RLG00000017884 RLG00000025036 RLG00000025174
rosa_multiflora	Rmu_co8443511.1_g000001 Rmu_sc0000449.1_g000044 Rmu_sc0001363.1_g000004 Rmu_sc0003763.1_g000005 Rmu_sc0005033.1_g000004 Rmu_sc0019318.1_g000002
rosa_roxburghii	Rroxscaffold_2G00132510 Rroxscaffold_6G00419750 Rroxscaffold_6G00421250
rosa_rugosa	Rorug02G0172800 Rorug02G0172800 Rorug03G0025200 Rorug03G0040700 Rorug03G0040800 Rorug03G0040900 Rorug03G0041000 Rorug03G0041000 Rorug03G0041100
rosa_samantha	Rh2AG225800 Rh2BG237500 Rh2CG228800 Rh2DG233300 Rh3AG086000 Rh3AG098500 Rh3BG088700 Rh3BG101700 Rh3CG089300 Rh3CG103800 Rh3DG089700 Rh3DG103000
rosa_wichuraiana	Rw2G017430 Rw3G007300 Rw3G008330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1955
Acc36I	ACCTGC	2 cut(s)	775, 2793
Acc65I	GGTACC	2 cut(s)	213, 679
AccB1I	GGYRCC	4 cut(s)	213, 679, 1838, 3187
AccB7I	CCANNNNNTGG	2 cut(s)	1724, 2344
AccBSI	CCGCTC	1 cut(s)	1914
AccII	CGCG	1 cut(s)	928
AccIII	TCCGGA	1 cut(s)	508
AclWI	GGATC	5 cut(s)	289, 931, 1891, 2131, 2366
AcsI	RAATTY	7 cut(s)	717, 979, 1338, 2051, 2480, 2882, 3233
AcuI	CTGAAG	3 cut(s)	85, 183, 809
AcyI	GRCGYC	2 cut(s)	47, 3188
AdeI	CACNNNGTG	1 cut(s)	2348
AfaI	GTAC	8 cut(s)	76, 215, 681, 932, 1833, 1947, 2307, 2541
AfiI	CCNNNNNNNGG	8 cut(s)	38, 63, 485, 1476, 1724, 2096, 2344, 3237
AflIII	ACRYGT	1 cut(s)	2179
AjnI	CCWGG	5 cut(s)	1213, 1788, 1834, 1971, 2892
AleI	CACNNNNGTG	1 cut(s)	147
Alw21I	GWGCWC	3 cut(s)	71, 461, 1457
Alw26I	GTCTC	4 cut(s)	521, 1239, 2398, 2594
AlwI	GGATC	5 cut(s)	289, 931, 1891, 2131, 2366
AlwNI	CAGNNNCTG	2 cut(s)	1145, 2344
Ama87I	CYCGRG	2 cut(s)	2498, 3183
Aor13HI	TCCGGA	1 cut(s)	508
AoxI	GGCC	4 cut(s)	447, 1153, 1467, 3227
ApeKI	GCWGC	7 cut(s)	641, 1028, 1067, 1079, 1118, 1519, 2032
ApoI	RAATTY	7 cut(s)	717, 979, 1338, 2051, 2480, 2882, 3233
ArsI	GACNNNNNNTTYG	2 cut(s)	2486, 2518
Asp700I	GAANNNNTTC	1 cut(s)	2728
Asp718I	GGTACC	2 cut(s)	213, 679
AspLEI	GCGC	2 cut(s)	232, 3190
AspS9I	GGNCC	4 cut(s)	448, 960, 1735, 3228
AsuC2I	CCSGG	1 cut(s)	263
AsuHPI	GGTGA	3 cut(s)	673, 1637, 3175
AvaI	CYCGRG	2 cut(s)	2498, 3183
AvaII	GGWCC	2 cut(s)	960, 1735
BanI	GGYRCC	4 cut(s)	213, 679, 1838, 3187
BanII	GRGCYC	3 cut(s)	71, 461, 1334
BbsI	GAAGAC	3 cut(s)	1622, 2874, 3054
Bbv12I	GWGCWC	3 cut(s)	71, 461, 1457
BbvI	GCAGC	7 cut(s)	628, 1015, 1054, 1066, 1105, 1531, 2019
BciT130I	CCWGG	5 cut(s)	1215, 1790, 1836, 1973, 2894
BciVI	GTATCC	3 cut(s)	259, 418, 1300
BclI	TGATCA	4 cut(s)	7, 1690, 2173, 2506
BcnI	CCSGG	1 cut(s)	263
BcoDI	GTCTC	4 cut(s)	521, 1239, 2398, 2594
BfaI	CTAG	4 cut(s)	1563, 2078, 2166, 2750
BfmI	CTRYAG	1 cut(s)	60
BfoI	RGCGCY	2 cut(s)	233, 3191
BfuAI	ACCTGC	2 cut(s)	775, 2793
BfuI	GTATCC	3 cut(s)	259, 418, 1300
Bme1390I	CCNGG	6 cut(s)	263, 1215, 1790, 1836, 1973, 2894
Bme18I	GGWCC	2 cut(s)	960, 1735
BmeT110I	CYCGRG	2 cut(s)	2498, 3183
BmgT120I	GGNCC	4 cut(s)	448, 960, 1735, 3228
BmrFI	CCNGG	6 cut(s)	263, 1215, 1790, 1836, 1973, 2894
BmrI	ACTGGG	2 cut(s)	2085, 2354
BmsI	GCATC	8 cut(s)	160, 332, 372, 964, 1531, 1849, 2404, 2766
BmuI	ACTGGG	2 cut(s)	2085, 2354
BpiI	GAAGAC	3 cut(s)	1622, 2874, 3054
BpmI	CTGGAG	5 cut(s)	55, 445, 1092, 2915, 3063
Bpu10I	CCTNAGC	1 cut(s)	684
BpuEI	CTTGAG	7 cut(s)	380, 1005, 1178, 1856, 1966, 2669, 2771
BpuMI	CCSGG	1 cut(s)	263
BsaAI	YACGTR	1 cut(s)	78
BsaBI	GATNNNNATC	1 cut(s)	2636
BsaHI	GRCGYC	2 cut(s)	47, 3188
BsaJI	CCNNGG	6 cut(s)	615, 1788, 2298, 2553, 2938, 3018
BsaWI	WCCGGW	2 cut(s)	508, 1172
BsaXI	ACNNNNNCTCC	2 cut(s)	58, 88
Bsc4I	CCNNNNNNNGG	8 cut(s)	38, 63, 485, 1476, 1724, 2096, 2344, 3237
Bse118I	RCCGGY	2 cut(s)	31, 1574
Bse1I	ACTGG	7 cut(s)	72, 462, 900, 1150, 2091, 2133, 2349
Bse8I	GATNNNNATC	1 cut(s)	2636
BseAI	TCCGGA	1 cut(s)	508
BseBI	CCWGG	5 cut(s)	1215, 1790, 1836, 1973, 2894
BseDI	CCNNGG	6 cut(s)	615, 1788, 2298, 2553, 2938, 3018
BseJI	GATNNNNATC	1 cut(s)	2636
BseLI	CCNNNNNNNGG	8 cut(s)	38, 63, 485, 1476, 1724, 2096, 2344, 3237
BseMII	CTCAG	6 cut(s)	603, 1101, 1489, 1992, 2939, 3066
BseNI	ACTGG	7 cut(s)	72, 462, 900, 1150, 2091, 2133, 2349
BseRI	GAGGAG	5 cut(s)	180, 682, 1653, 3050, 3158
BseXI	GCAGC	7 cut(s)	628, 1015, 1054, 1066, 1105, 1531, 2019
BseYI	CCCAGC	2 cut(s)	1448, 1610
BsgI	GTGCAG	1 cut(s)	2249
Bsh1236I	CGCG	1 cut(s)	928
BshFI	GGCC	4 cut(s)	449, 1155, 1469, 3229
BshNI	GGYRCC	4 cut(s)	213, 679, 1838, 3187
BsiHKAI	GWGCWC	3 cut(s)	71, 461, 1457
BsiHKCI	CYCGRG	2 cut(s)	2498, 3183
BsiSI	CCGG	5 cut(s)	32, 263, 509, 1173, 1575
BslFI	GGGAC	4 cut(s)	424, 1721, 2073, 2552
BslI	CCNNNNNNNGG	8 cut(s)	38, 63, 485, 1476, 1724, 2096, 2344, 3237
BsmAI	GTCTC	4 cut(s)	521, 1239, 2398, 2594
BsmFI	GGGAC	4 cut(s)	424, 1721, 2073, 2552
BsmI	GAATGC	2 cut(s)	644, 1192
BsnI	GGCC	4 cut(s)	449, 1155, 1469, 3229
BsoBI	CYCGRG	2 cut(s)	2498, 3183
Bsp1286I	GDGCHC	4 cut(s)	71, 461, 1334, 1457
Bsp13I	TCCGGA	1 cut(s)	508
Bsp1407I	TGTACA	1 cut(s)	2305
BspANI	GGCC	4 cut(s)	449, 1155, 1469, 3229
BspCNI	CTCAG	6 cut(s)	602, 1100, 1488, 1993, 2938, 3065
BspEI	TCCGGA	1 cut(s)	508
BspFNI	CGCG	1 cut(s)	928
BspHI	TCATGA	1 cut(s)	1855
BspMAI	CTGCAG	1 cut(s)	64
BspMI	ACCTGC	2 cut(s)	775, 2793
BspPI	GGATC	5 cut(s)	289, 931, 1891, 2131, 2366
BspQI	GCTCTTC	1 cut(s)	2385
BspT107I	GGYRCC	4 cut(s)	213, 679, 1838, 3187
BsrBI	CCGCTC	1 cut(s)	1914
BsrFI	RCCGGY	2 cut(s)	31, 1574
BsrGI	TGTACA	1 cut(s)	2305
BsrI	ACTGG	7 cut(s)	72, 462, 900, 1150, 2091, 2133, 2349
BssAI	RCCGGY	2 cut(s)	31, 1574
BssECI	CCNNGG	6 cut(s)	615, 1788, 2298, 2553, 2938, 3018
BssNI	GRCGYC	2 cut(s)	47, 3188
BssT1I	CCWWGG	2 cut(s)	2298, 2938
Bst2UI	CCWGG	5 cut(s)	1215, 1790, 1836, 1973, 2894
Bst6I	CTCTTC	6 cut(s)	1754, 2385, 3027, 3051, 3075, 3135
BstACI	GRCGYC	2 cut(s)	47, 3188
BstAUI	TGTACA	1 cut(s)	2305
BstBAI	YACGTR	1 cut(s)	78
BstC8I	GCNNGC	9 cut(s)	107, 351, 1051, 1257, 1435, 1540, 1572, 2226, 3100
BstDSI	CCRYGG	1 cut(s)	615
BstFNI	CGCG	1 cut(s)	928
BstH2I	RGCGCY	2 cut(s)	233, 3191
BstHHI	GCGC	2 cut(s)	232, 3190
BstMAI	GTCTC	4 cut(s)	521, 1239, 2398, 2594
BstNI	CCWGG	5 cut(s)	1215, 1790, 1836, 1973, 2894
BstNSI	RCATGY	2 cut(s)	389, 2213
BstSCI	CCNGG	6 cut(s)	261, 1213, 1788, 1834, 1971, 2892
BstSFI	CTRYAG	1 cut(s)	60
BstSNI	TACGTA	1 cut(s)	78
BstUI	CGCG	1 cut(s)	928
BstV1I	GCAGC	7 cut(s)	628, 1015, 1054, 1066, 1105, 1531, 2019
BstV2I	GAAGAC	3 cut(s)	1622, 2874, 3054
BstX2I	RGATCY	1 cut(s)	294
BstXI	CCANNNNNNTGG	1 cut(s)	2144
BstYI	RGATCY	1 cut(s)	294
BsuI	GTATCC	3 cut(s)	259, 418, 1300
BsuRI	GGCC	4 cut(s)	449, 1155, 1469, 3229
BtgI	CCRYGG	1 cut(s)	615
BtsI	GCAGTG	1 cut(s)	57
BtsIMutI	CAGTG	9 cut(s)	57, 154, 731, 907, 1292, 2126, 2342, 2908, 3142
BveI	ACCTGC	2 cut(s)	775, 2793
Cac8I	GCNNGC	9 cut(s)	107, 351, 1051, 1257, 1435, 1540, 1572, 2226, 3100
CaiI	CAGNNNCTG	2 cut(s)	1145, 2344
CciI	TCATGA	1 cut(s)	1855
CfoI	GCGC	2 cut(s)	232, 3190
Cfr10I	RCCGGY	2 cut(s)	31, 1574
Cfr13I	GGNCC	4 cut(s)	448, 960, 1735, 3228
CseI	GACGC	1 cut(s)	36
Csp6I	GTAC	8 cut(s)	75, 214, 680, 931, 1832, 1946, 2306, 2540
CviQI	GTAC	8 cut(s)	75, 214, 680, 931, 1832, 1946, 2306, 2540
DinI	GGCGCC	1 cut(s)	3189
DraIII	CACNNNGTG	1 cut(s)	2348
Eam1104I	CTCTTC	6 cut(s)	1754, 2385, 3027, 3051, 3075, 3135
EarI	CTCTTC	6 cut(s)	1754, 2385, 3027, 3051, 3075, 3135
EciI	GGCGGA	1 cut(s)	852
Ecl136II	GAGCTC	2 cut(s)	69, 459
Eco105I	TACGTA	1 cut(s)	78
Eco130I	CCWWGG	2 cut(s)	2298, 2938
Eco24I	GRGCYC	3 cut(s)	71, 461, 1334
Eco32I	GATATC	2 cut(s)	808, 1657
Eco47I	GGWCC	2 cut(s)	960, 1735
Eco53kI	GAGCTC	2 cut(s)	69, 459
Eco57I	CTGAAG	3 cut(s)	85, 183, 809
Eco88I	CYCGRG	2 cut(s)	2498, 3183
EcoICRI	GAGCTC	2 cut(s)	69, 459
EcoRI	GAATTC	1 cut(s)	2480
EcoRII	CCWGG	5 cut(s)	1213, 1788, 1834, 1971, 2892
EcoRV	GATATC	2 cut(s)	808, 1657
EcoT14I	CCWWGG	2 cut(s)	2298, 2938
EcoT22I	ATGCAT	2 cut(s)	387, 2215
EcoT38I	GRGCYC	3 cut(s)	71, 461, 1334
EgeI	GGCGCC	1 cut(s)	3189
EheI	GGCGCC	1 cut(s)	3189
ErhI	CCWWGG	2 cut(s)	2298, 2938
FalI	AAGNNNNNCTT	4 cut(s)	504, 536, 1518, 1550
FaqI	GGGAC	4 cut(s)	424, 1721, 2073, 2552
FauI	CCCGC	2 cut(s)	1547, 2703
FbaI	TGATCA	4 cut(s)	7, 1690, 2173, 2506
FriOI	GRGCYC	3 cut(s)	71, 461, 1334
FspBI	CTAG	4 cut(s)	1563, 2078, 2166, 2750
GlaI	GCGC	2 cut(s)	231, 3189
GsaI	CCCAGC	2 cut(s)	1452, 1614
GsuI	CTGGAG	5 cut(s)	55, 445, 1092, 2915, 3063
HaeII	RGCGCY	2 cut(s)	233, 3191
HaeIII	GGCC	4 cut(s)	449, 1155, 1469, 3229
HapII	CCGG	5 cut(s)	32, 263, 509, 1173, 1575
HgaI	GACGC	1 cut(s)	36
HhaI	GCGC	2 cut(s)	232, 3190
Hin1I	GRCGYC	2 cut(s)	47, 3188
Hin6I	GCGC	2 cut(s)	230, 3188
HinP1I	GCGC	2 cut(s)	230, 3188
HincII	GTYRAC	1 cut(s)	567
HindII	GTYRAC	1 cut(s)	567
HindIII	AAGCTT	3 cut(s)	518, 686, 996
HpaII	CCGG	5 cut(s)	32, 263, 509, 1173, 1575
HphI	GGTGA	3 cut(s)	673, 1637, 3175
Hpy166II	GTNNAC	5 cut(s)	567, 2185, 2449, 2644, 2742
Hpy8I	GTNNAC	5 cut(s)	567, 2185, 2449, 2644, 2742
Hpy99I	CGWCG	2 cut(s)	229, 661
HpyCH4IV	ACGT	3 cut(s)	77, 2179, 2187
HpySE526I	ACGT	3 cut(s)	77, 2179, 2187
Hsp92I	GRCGYC	2 cut(s)	47, 3188
HspAI	GCGC	2 cut(s)	230, 3188
KasI	GGCGCC	1 cut(s)	3187
Kpn2I	TCCGGA	1 cut(s)	508
KpnI	GGTACC	2 cut(s)	217, 683
Ksp22I	TGATCA	4 cut(s)	7, 1690, 2173, 2506
LguI	GCTCTTC	1 cut(s)	2385
Lsp1109I	GCAGC	7 cut(s)	628, 1015, 1054, 1066, 1105, 1531, 2019
LweI	GCATC	8 cut(s)	160, 332, 372, 964, 1531, 1849, 2404, 2766
MaeI	CTAG	4 cut(s)	1563, 2078, 2166, 2750
MaeII	ACGT	3 cut(s)	77, 2179, 2187
MaeIII	GTNAC	6 cut(s)	161, 574, 887, 1796, 2017, 2285
MbiI	CCGCTC	1 cut(s)	1914
MfeI	CAATTG	2 cut(s)	1620, 2815
MflI	RGATCY	1 cut(s)	294
MhlI	GDGCHC	4 cut(s)	71, 461, 1334, 1457
Mly113I	GGCGCC	1 cut(s)	3188
MmeI	TCCRAC	5 cut(s)	218, 312, 466, 901, 3156
Mph1103I	ATGCAT	2 cut(s)	387, 2215
MroI	TCCGGA	1 cut(s)	508
MroXI	GAANNNNTTC	1 cut(s)	2728
MseI	TTAA	7 cut(s)	777, 1014, 1043, 1061, 1179, 1827, 2424
MslI	CAYNNNNRTG	6 cut(s)	135, 147, 168, 1935, 2142, 2279
MspI	CCGG	5 cut(s)	32, 263, 509, 1173, 1575
MspR9I	CCNGG	6 cut(s)	263, 1215, 1790, 1836, 1973, 2894
MunI	CAATTG	2 cut(s)	1620, 2815
Mva1269I	GAATGC	2 cut(s)	644, 1192
MvaI	CCWGG	5 cut(s)	1215, 1790, 1836, 1973, 2894
MvnI	CGCG	1 cut(s)	928
NarI	GGCGCC	1 cut(s)	3188
NciI	CCSGG	1 cut(s)	263
NmeAIII	GCCGAG	1 cut(s)	2534
NmuCI	GTSAC	1 cut(s)	161
NsiI	ATGCAT	2 cut(s)	387, 2215
NspI	RCATGY	2 cut(s)	389, 2213
OliI	CACNNNNGTG	1 cut(s)	147
PaeR7I	CTCGAG	1 cut(s)	2498
PagI	TCATGA	1 cut(s)	1855
PciSI	GCTCTTC	1 cut(s)	2385
PctI	GAATGC	2 cut(s)	644, 1192
PdmI	GAANNNNTTC	1 cut(s)	2728
PfeI	GAWTC	8 cut(s)	1583, 1681, 2083, 2729, 2809, 2906, 2912, 2969
PflFI	GACNNNGTC	1 cut(s)	655
PflMI	CCANNNNNTGG	2 cut(s)	1724, 2344
PfoI	TCCNGGA	1 cut(s)	261
PluTI	GGCGCC	1 cut(s)	3191
Ppu21I	YACGTR	1 cut(s)	78
PsiI	TTATAA	1 cut(s)	1955
Psp124BI	GAGCTC	2 cut(s)	71, 461
Psp6I	CCWGG	5 cut(s)	1213, 1788, 1834, 1971, 2892
PspFI	CCCAGC	2 cut(s)	1448, 1610
PspGI	CCWGG	5 cut(s)	1213, 1788, 1834, 1971, 2892
PspPI	GGNCC	4 cut(s)	448, 960, 1735, 3228
PspXI	VCTCGAGB	1 cut(s)	2498
PsrI	GAACNNNNNNTAC	2 cut(s)	872, 904
PstI	CTGCAG	1 cut(s)	64
PstNI	CAGNNNCTG	2 cut(s)	1145, 2344
PsuI	RGATCY	1 cut(s)	294
PsyI	GACNNNGTC	1 cut(s)	655
RsaI	GTAC	8 cut(s)	76, 215, 681, 932, 1833, 1947, 2307, 2541
RsaNI	GTAC	8 cut(s)	75, 214, 680, 931, 1832, 1946, 2306, 2540
RseI	CAYNNNNRTG	6 cut(s)	135, 147, 168, 1935, 2142, 2279
SacI	GAGCTC	2 cut(s)	71, 461
SapI	GCTCTTC	1 cut(s)	2385
SaqAI	TTAA	7 cut(s)	777, 1014, 1043, 1061, 1179, 1827, 2424
Sau96I	GGNCC	4 cut(s)	448, 960, 1735, 3228
ScrFI	CCNGG	6 cut(s)	263, 1215, 1790, 1836, 1973, 2894
SduI	GDGCHC	4 cut(s)	71, 461, 1334, 1457
SfaNI	GCATC	8 cut(s)	160, 332, 372, 964, 1531, 1849, 2404, 2766
SfcI	CTRYAG	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	3189
Sfr274I	CTCGAG	1 cut(s)	2498
SinI	GGWCC	2 cut(s)	960, 1735
SlaI	CTCGAG	1 cut(s)	2498
SmiMI	CAYNNNNRTG	6 cut(s)	135, 147, 168, 1935, 2142, 2279
SmlI	CTYRAG	8 cut(s)	395, 1020, 1157, 1871, 1981, 2498, 2648, 2786
SmoI	CTYRAG	8 cut(s)	395, 1020, 1157, 1871, 1981, 2498, 2648, 2786
SnaBI	TACGTA	1 cut(s)	78
SspDI	GGCGCC	1 cut(s)	3187
SspMI	CTAG	4 cut(s)	1563, 2078, 2166, 2750
SstI	GAGCTC	2 cut(s)	71, 461
StyD4I	CCNGG	6 cut(s)	261, 1213, 1788, 1834, 1971, 2892
StyI	CCWWGG	2 cut(s)	2298, 2938
TaiI	ACGT	3 cut(s)	80, 2182, 2190
TaqI	TCGA	3 cut(s)	455, 561, 2499
TatI	WGTACW	2 cut(s)	1945, 2305
TauI	GCSGC	3 cut(s)	112, 1592, 1971
TfiI	GAWTC	8 cut(s)	1583, 1681, 2083, 2729, 2809, 2906, 2912, 2969
Tru1I	TTAA	7 cut(s)	777, 1014, 1043, 1061, 1179, 1827, 2424
Tru9I	TTAA	7 cut(s)	777, 1014, 1043, 1061, 1179, 1827, 2424
TscAI	CASTG	9 cut(s)	64, 154, 738, 907, 1299, 2133, 2349, 2908, 3142
TseFI	GTSAC	1 cut(s)	161
TseI	GCWGC	7 cut(s)	641, 1028, 1067, 1079, 1118, 1519, 2032
Tsp45I	GTSAC	1 cut(s)	161
TspDTI	ATGAA	9 cut(s)	153, 756, 1422, 1506, 1716, 1896, 2249, 2551, 2851
TspGWI	ACGGA	5 cut(s)	604, 761, 1528, 1805, 2333
TspRI	CASTG	9 cut(s)	64, 154, 738, 907, 1299, 2133, 2349, 2908, 3142
Tth111I	GACNNNGTC	1 cut(s)	655
Van91I	CCANNNNNTGG	2 cut(s)	1724, 2344
VpaK11BI	GGWCC	2 cut(s)	960, 1735
XapI	RAATTY	7 cut(s)	717, 979, 1338, 2051, 2480, 2882, 3233
XbaI	TCTAGA	1 cut(s)	2077
XceI	RCATGY	2 cut(s)	389, 2213
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2345
XhoI	CTCGAG	1 cut(s)	2498
XmnI	GAANNNNTTC	1 cut(s)	2728
XspI	CTAG	4 cut(s)	1563, 2078, 2166, 2750
Zsp2I	ATGCAT	2 cut(s)	387, 2215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.