AT4G10710

FACT complex subunit (SPT16/CDC68)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001181
Physical Location & Seq
Reverse (-)
6601903 .. 6606357
4455 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10710.1

Sequence Viewer

Length: 3225 bp
ATGGCAGACTCTCGGAATGGTAATGCCCGAGCCCCTCCTAGTGGAGTGCCTCCAAAAGCTGGGAATACTTATTCCATAGATGTTAAAAACTTTATCTCACGTGCAAGAGCATTGTATGAACACTGGAAGAAGCACAGTGCAGACTTATGGGGATCTGCCGACGCTCTTGCCATTGCTACCCCTCCTGCTTCTGATGATCTGCGTTACCTGAAATCTTCAGCGTTGAATATTTGGCTTCTTGGGTATGAGTTTCCAGATACAATTATGGTTTTTACCAAGAAGCAGATCCATTTCCTGTGTAGTAGGAACAAGGCTTCTCTACTTGAAGTTGTGAAAAAACCTGCACATGATGAGTTGAAGCTTGATGTTATCATGCATGTTAAGCCTAAGGGTGATGATGGAACAGGGCTGATGGATGCCATATTTCGTGCCATCCGTGATCTATCCCGGGGTGATGGAAATGATTCACAAGTTGTTGGACACATTGCTCGTGAAGCCCCTGAAGGTAAGCTTCTGGAGACATGGACTGAGAGGCTAAAGAATGCAAACTTCCAGTTCGTAGATATAACTGGGGGGCTGTCTGATCTATTTGCTGTTAAAGACGATACTGAGGTCATGAGTGTGAAGAAAGCTGCGTATCTAGCCTACAGTGTGATGAAAAATGTTGTTGTTCCAAATCTTGAGAGTGCCATTGATGAGGAGAAAGACGTCACCCACTCTGCCCTGATGGATCTCACTGAGAAAGCGATACTTGAGCCAACTAAGGCCAGTGTGAAACTGAAGCCAGAGAATGTTGACATATGCTACCCTCCGATATTTCAAAGCGGAGGCAAGTTTGATCTCAAACCAAGTGCTGCAAGCAATGATGAACTGCTCACTTATGATCCAGCGAGTATCATAATATGTGCTGTTGGTGCTAGGTATAATAGTTATTGCTCAAATGTTGCCAGGACCTACCTAATAGATGCAACTTCTCTCCAGAGCAAGGCATATGAGGTTCTTCTCAAGGCACATGAGGCTGCTATTGATGCTTTGAGGTCAGGAAGAAAGATAAACACTGTCTATCAAGCTGCCCTTTCTGTTGTTGAAAAGAATGCCCCTGAGTTCGTTGACAAGCTAACTAAATCTGCTGGGACTGGTATCGGTCTGGAATTCCGAGAGTCAGGATTAAATATAAATGCAAAGAATGATAAAGTATTGAGACCGAAGATGGCATTTAATGTGTCTCTTGGTTTCCAGAACTTGGAATGTGAGTCAGAAAGCCGCAGCAAGAACAAGAAATTCTCACTTTTGCTAGCTGACACAGTTCTCGTCACAGATCAGAAACCGGAGCTTTTAACCAAGTGCTCTAAATCTGTTAAGGATGTGGCTTACTCCTTTAAAGAGGATGAAGAAGAAGAGAAACCGCGCAAGAAAGCTAGGACCAGTGGTTCTGAGAACTACATTACCAAGACAGCTCTTAGATCAGATGATCATGTGGTGTCTAAAGAAGAGCTACGGAAGCAGCACCAGGCAGAGCTGGCACGCCAGAAAAATGAAGAAACTGCCAGAAGGCTTGCTGGTGACAGTTCTGGGGCGGGAGACAGCCGGTCTACTGCAAAGACTTCAGCTGATGTGGTTGCCTACAAGAATGTTAACGACATGCCCCATAAGGAATTGATGATCCAGGTTGATACGAGAAACGAGGCTGTATTGTTACCCATCTATGGGAGCTTGGTTCCATTCCATGTGGCTACAATAAGAACAGTGTCAGGCAATCAAGATACCAACCGAAACTGCTACATCCGTATCATTTTCAATGTCCCGGGTACACCCTTCAACCCTCATGACTCAAACTCTTTGAAAAACCAGGGTGCTATCTACCTCAAGGAGGTTTCATTCCGGACCAAGGATTCAAGGCATAGCAGCGAAGTAACTCAACAGATTAAGACCCTTAGACGGCAAGTCATGGCCAGGGAGTCAGAGAGAGCTGAAAGGGCGACATTGGTGACCCAAGAGAAACTTCAGCTTGCAGGGAACAAGTTCAAACCCCTCAGGTTGTCCGAGCTGTGGATCCGTCCGCCTTTTAGTGGGCGCAAGAAGATTCCTGGGACACTCGAAGCTCATGCAAATGGTTTCAGGTATTCAACTACCAGGCCTGACGAGCGCGTGGATGTCCTGTTTGCAAACATAAAGCATGCGTTTTTCCAGCCTGCTGAGAAGGAGATGATCACCCTCCTTCATTTTCATTTGCACAACCACATCATGGTAGGAACTAAGAAGACAAAGGACGTTCAATTCTATGTGGAGGTAATGGATGTTGTGCAGTCTTTAGGTGGTGGGAGGAGATCAGCCTATGATCCAGATGAGATTGACGAAGAACAGAGGGAGCGCGATAGGAAGAACAAGATCAACATGGATTTCAATCATTTTGCAAACCGGGTGAATGATATGTGGCAACTACCACAGTTTGCAAGTCTGGACCTTGAGTTTGATCAACCTCTGAGAGAGCTTGGTTTCCATGGTGTTCCGCACAAGACATCTGCATTCATAATACCGACCTCCAGCTGCTTAGTTGAGCTGATAGAATACCCGTTCCTCGTGGTGAGTCTGAGTGAGATTGAGATTGTGAATCTTGAGAGAGTTGGGTTTGGGCAGAAGAACTTTGACATGGCCATCATCTTCAAGGACTTCAAAAAAGATGTTCTCAGGGTTGACTCAGTTCCCACAAGCTCCCTAGAGGGGATAAAGGAGTGGCTAGACACCACAGATATAAAGTACTACGAGAGCAAACTGAATCTGAACTGGAGACAGATCCTAAAGACGATCACAGATGATCCGCAGAGCTTTATAGATGATGGAGGATGGGAGTTTCTGAACCTGGACGGAAGCGATTCAGAATCTGGCGGGTCTGAGGAGTCAGACAAAGGATATGAACCATCAGATGTAGAGGTTGAGTCTGAGTCAGAGGACGAGGCTTCAGAGAGTGAGTCACTGGTGGAGTCAGACGATGATGAAGAAGAGGACTCAGAGCAAGAGTCAGAGGAAGAGAAAGGTAAGACTTGGGATGAACTGGAGAGAGAAGCTACTAATGCAGACAGAGAGCATGGAGTTGAGTCTGATAGCGAGGAAGAGAGGAAGAGAAGAAAGATGAAAGCGTTTGGGAAATCGCGACCTGGAACTAGCGGTGGAGGTGGTAGTAGCAGCATGAAGAACATGCCACCATCTAAACGCAAGCACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1074

Amino Acids

120.58

Weight (kDa)

5.7

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FACT-Spt16_Nlob PF14826 26 - 190 7.4e-50 FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24 PF00557 209 - 440 6.3e-30 Metallopeptidase family M24
SPT16 PF08644 555 - 668 8.9e-37 FACT complex subunit (SPT16/CDC68)
PH_SPT16 PF24824 679 - 808 2e-54 FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle PF08512 835 - 922 2.6e-22 Histone chaperone Rttp106-like, middle domain
SPT16_C PF21091 963 - 1043 4.8e-18 FACT complex subunit SPT16, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 711
Acc36I ACCTGC 1 cut(s) 349
AccB7I CCANNNNNTGG 3 cut(s) 59, 1243, 2245
AccI GTMKAC 1 cut(s) 1592
AccII CGCG 4 cut(s) 1408, 2148, 2373, 3151
AccIII TCCGGA 1 cut(s) 1881
AciI CCGC 9 cut(s) 825, 1264, 1406, 1577, 2060, 2510, 2819, 2886, 3165
AcoI YGGCCR 2 cut(s) 1950, 2652
AcsI RAATTY 2 cut(s) 1151, 1280
AcuI CTGAAG 6 cut(s) 201, 522, 800, 1590, 1988, 2943
AcvI CACGTG 1 cut(s) 101
AcyI GRCGYC 1 cut(s) 708
AfaI GTAC 2 cut(s) 1810, 2759
AhdI GACNNNNNGTC 2 cut(s) 1588, 1943
AjnI CCWGG 9 cut(s) 947, 1509, 1665, 1848, 1952, 2086, 2132, 2859, 3154
AjuI GAANNNNNNNTTGG 2 cut(s) 1986, 2018
AloI GAACNNNNNNTCC 2 cut(s) 1414, 1446
Alw21I GWGCWC 1 cut(s) 1349
Alw26I GTCTC 5 cut(s) 512, 1195, 1230, 1575, 2782
AlwNI CAGNNNCTG 1 cut(s) 2882
Ama87I CYCGRG 3 cut(s) 27, 447, 1804
Aor13HI TCCGGA 1 cut(s) 1881
AoxI GGCC 4 cut(s) 765, 1950, 2135, 2652
ApeKI GCWGC 9 cut(s) 632, 854, 1019, 1070, 1266, 1504, 1905, 2547, 3183
ApoI RAATTY 2 cut(s) 1151, 1280
ArsI GACNNNNNNTTYG 2 cut(s) 2453, 2485
Asp700I GAANNNNTTC 3 cut(s) 463, 2021, 2872
AspLEI GCGC 4 cut(s) 1410, 2076, 2148, 2373
AspS9I GGNCC 4 cut(s) 951, 1422, 1884, 2461
AsuC2I CCSGG 5 cut(s) 448, 449, 1805, 1806, 2420
AsuHPI GGTGA 8 cut(s) 404, 464, 703, 1574, 1999, 2203, 2434, 2596
AsuNHI GCTAGC 1 cut(s) 1294
AvaI CYCGRG 3 cut(s) 27, 447, 1804
AvaII GGWCC 4 cut(s) 951, 1422, 1884, 2461
AxyI CCTNAGG 2 cut(s) 387, 2033
BaeI ACNNNNGTAYC 4 cut(s) 597, 630, 1771, 1804
BalI TGGCCA 2 cut(s) 1952, 2654
BamHI GGATCC 1 cut(s) 2052
BanII GRGCYC 1 cut(s) 34
BauI CACGAG 2 cut(s) 489, 2579
BbrPI CACGTG 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 2267
Bbv12I GWGCWC 1 cut(s) 1349
BbvI GCAGC 9 cut(s) 619, 841, 1006, 1057, 1278, 1516, 1917, 2534, 3195
BceAI ACGGC 1 cut(s) 1955
BcgI CGANNNNNNTGC 4 cut(s) 149, 183, 2087, 2121
BciT130I CCWGG 9 cut(s) 949, 1511, 1667, 1850, 1954, 2088, 2134, 2861, 3156
BclI TGATCA 3 cut(s) 1471, 2208, 2473
BcnI CCSGG 5 cut(s) 448, 449, 1805, 1806, 2420
BcoDI GTCTC 5 cut(s) 512, 1195, 1230, 1575, 2782
BfaI CTAG 8 cut(s) 39, 641, 918, 1295, 1419, 2717, 2738, 3162
BfmI CTRYAG 1 cut(s) 646
BfuAI ACCTGC 1 cut(s) 349
BmcAI AGTACT 1 cut(s) 2759
Bme18I GGWCC 4 cut(s) 951, 1422, 1884, 2461
BmeRI GACNNNNNGTC 2 cut(s) 1588, 1943
BmeT110I CYCGRG 3 cut(s) 27, 447, 1804
BmgT120I GGNCC 4 cut(s) 951, 1422, 1884, 2461
BmiI GGNNCC 2 cut(s) 1719, 2054
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 3 cut(s) 406, 955, 1018
BmtI GCTAGC 1 cut(s) 1298
BmuI ACTGGG 1 cut(s) 579
BpiI GAAGAC 1 cut(s) 2267
BpmI CTGGAG 5 cut(s) 536, 962, 2527, 2806, 3074
BpuEI CTTGAG 6 cut(s) 701, 773, 989, 1850, 2486, 2636
BpuMI CCSGG 5 cut(s) 448, 449, 1805, 1806, 2420
BsaAI YACGTR 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 2403
BsaHI GRCGYC 1 cut(s) 708
BsaI GGTCTC 1 cut(s) 1195
BsaJI CCNNGG 8 cut(s) 447, 448, 1804, 1849, 1887, 1953, 2087, 2500
BsaWI WCCGGW 2 cut(s) 1327, 1881
Bse118I RCCGGY 1 cut(s) 1587
Bse1I ACTGG 9 cut(s) 128, 553, 574, 768, 1141, 1425, 2789, 2979, 3057
Bse21I CCTNAGG 2 cut(s) 387, 2033
Bse3DI GCAATG 3 cut(s) 171, 483, 868
Bse8I GATNNNNATC 1 cut(s) 2403
BseAI TCCGGA 1 cut(s) 1881
BseBI CCWGG 9 cut(s) 949, 1511, 1667, 1850, 1954, 2088, 2134, 2861, 3156
BseDI CCNNGG 8 cut(s) 447, 448, 1804, 1849, 1887, 1953, 2087, 2500
BseGI GGATG 9 cut(s) 421, 432, 1369, 1393, 1782, 2158, 2302, 2849, 3052
BseJI GATNNNNATC 1 cut(s) 2403
BseMI GCAATG 3 cut(s) 171, 483, 868
BseNI ACTGG 9 cut(s) 128, 553, 574, 768, 1141, 1425, 2789, 2979, 3057
BseRI GAGGAG 3 cut(s) 713, 2338, 2909
BseXI GCAGC 9 cut(s) 619, 841, 1006, 1057, 1278, 1516, 1917, 2534, 3195
BseYI CCCAGC 2 cut(s) 59, 1130
BsgI GTGCAG 3 cut(s) 159, 327, 2324
Bsh1236I CGCG 4 cut(s) 1408, 2148, 2373, 3151
BshFI GGCC 4 cut(s) 767, 1952, 2137, 2654
BsiHKAI GWGCWC 1 cut(s) 1349
BsiHKCI CYCGRG 3 cut(s) 27, 447, 1804
BsiSI CCGG 6 cut(s) 448, 1328, 1588, 1805, 1882, 2419
BslFI GGGAC 3 cut(s) 1147, 1787, 2104
BsmAI GTCTC 5 cut(s) 512, 1195, 1230, 1575, 2782
BsmFI GGGAC 3 cut(s) 1147, 1787, 2104
BsmI GAATGC 3 cut(s) 547, 1099, 2525
BsnI GGCC 4 cut(s) 767, 1952, 2137, 2654
Bso31I GGTCTC 1 cut(s) 1195
BsoBI CYCGRG 3 cut(s) 27, 447, 1804
Bsp1286I GDGCHC 2 cut(s) 34, 1349
Bsp13I TCCGGA 1 cut(s) 1881
Bsp19I CCATGG 1 cut(s) 2500
Bsp68I TCGCGA 1 cut(s) 3151
BspACI CCGC 9 cut(s) 825, 1264, 1406, 1577, 2060, 2510, 2819, 2886, 3165
BspANI GGCC 4 cut(s) 767, 1952, 2137, 2654
BspEI TCCGGA 1 cut(s) 1881
BspFNI CGCG 4 cut(s) 1408, 2148, 2373, 3151
BspHI TCATGA 2 cut(s) 615, 1825
BspLI GGNNCC 2 cut(s) 1719, 2054
BspMI ACCTGC 1 cut(s) 349
BspOI GCTAGC 1 cut(s) 1298
BspQI GCTCTTC 1 cut(s) 1485
BspTNI GGTCTC 1 cut(s) 1195
BsrDI GCAATG 3 cut(s) 171, 483, 868
BsrFI RCCGGY 1 cut(s) 1587
BsrI ACTGG 9 cut(s) 128, 553, 574, 768, 1141, 1425, 2789, 2979, 3057
BssAI RCCGGY 1 cut(s) 1587
BssECI CCNNGG 8 cut(s) 447, 448, 1804, 1849, 1887, 1953, 2087, 2500
BssNI GRCGYC 1 cut(s) 708
BssSI CACGAG 2 cut(s) 489, 2579
BssT1I CCWWGG 2 cut(s) 1887, 2500
Bst2BI CACGAG 2 cut(s) 489, 2579
Bst2UI CCWGG 9 cut(s) 949, 1511, 1667, 1850, 1954, 2088, 2134, 2861, 3156
Bst4CI ACNGT 7 cut(s) 137, 650, 1060, 1306, 1568, 1747, 2448
Bst6I CTCTTC 6 cut(s) 1392, 1485, 2994, 3021, 3105, 3113
BstACI GRCGYC 1 cut(s) 708
BstBAI YACGTR 1 cut(s) 101
BstC8I GCNNGC 9 cut(s) 859, 1296, 1521, 1525, 1557, 2010, 2178, 2193, 3215
BstDSI CCRYGG 1 cut(s) 2500
BstEII GGTNACC 1 cut(s) 1987
BstENI CCTNNNNNAGG 1 cut(s) 1868
BstF5I GGATG 9 cut(s) 421, 432, 1369, 1393, 1782, 2158, 2302, 2849, 3052
BstFNI CGCG 4 cut(s) 1408, 2148, 2373, 3151
BstHHI GCGC 4 cut(s) 1410, 2076, 2148, 2373
BstMAI GTCTC 5 cut(s) 512, 1195, 1230, 1575, 2782
BstNI CCWGG 9 cut(s) 949, 1511, 1667, 1850, 1954, 2088, 2134, 2861, 3156
BstNSI RCATGY 4 cut(s) 380, 1645, 2180, 3199
BstPI GGTNACC 1 cut(s) 1987
BstSFI CTRYAG 1 cut(s) 646
BstUI CGCG 4 cut(s) 1408, 2148, 2373, 3151
BstV1I GCAGC 9 cut(s) 619, 841, 1006, 1057, 1278, 1516, 1917, 2534, 3195
BstV2I GAAGAC 1 cut(s) 2267
BstX2I RGATCY 5 cut(s) 152, 285, 730, 2052, 2793
BstYI RGATCY 5 cut(s) 152, 285, 730, 2052, 2793
Bsu36I CCTNAGG 2 cut(s) 387, 2033
BsuRI GGCC 4 cut(s) 767, 1952, 2137, 2654
BtgI CCRYGG 1 cut(s) 2500
BtsCI GGATG 9 cut(s) 421, 432, 1369, 1393, 1782, 2158, 2302, 2849, 3052
BtsIMutI CAGTG 9 cut(s) 121, 142, 655, 735, 775, 1056, 1432, 1752, 2972
BtuMI TCGCGA 1 cut(s) 3151
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 9 cut(s) 859, 1296, 1521, 1525, 1557, 2010, 2178, 2193, 3215
CaiI CAGNNNCTG 1 cut(s) 2882
CciI TCATGA 2 cut(s) 615, 1825
CfoI GCGC 4 cut(s) 1410, 2076, 2148, 2373
Cfr10I RCCGGY 1 cut(s) 1587
Cfr13I GGNCC 4 cut(s) 951, 1422, 1884, 2461
Cfr9I CCCGGG 2 cut(s) 447, 1804
CseI GACGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 1809, 2758
CviQI GTAC 2 cut(s) 1809, 2758
DraI TTTAAA 1 cut(s) 1381
DriI GACNNNNNGTC 2 cut(s) 1588, 1943
EaeI YGGCCR 2 cut(s) 1950, 2652
Eam1104I CTCTTC 6 cut(s) 1392, 1485, 2994, 3021, 3105, 3113
Eam1105I GACNNNNNGTC 2 cut(s) 1588, 1943
EarI CTCTTC 6 cut(s) 1392, 1485, 2994, 3021, 3105, 3113
EciI GGCGGA 1 cut(s) 2049
Eco130I CCWWGG 2 cut(s) 1887, 2500
Eco147I AGGCCT 1 cut(s) 2137
Eco24I GRGCYC 1 cut(s) 34
Eco31I GGTCTC 1 cut(s) 1195
Eco47I GGWCC 4 cut(s) 951, 1422, 1884, 2461
Eco57I CTGAAG 6 cut(s) 201, 522, 800, 1590, 1988, 2943
Eco72I CACGTG 1 cut(s) 101
Eco81I CCTNAGG 2 cut(s) 387, 2033
Eco88I CYCGRG 3 cut(s) 27, 447, 1804
Eco91I GGTNACC 1 cut(s) 1987
EcoNI CCTNNNNNAGG 1 cut(s) 1868
EcoO109I RGGNCCY 1 cut(s) 951
EcoO65I GGTNACC 1 cut(s) 1987
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 9 cut(s) 947, 1509, 1665, 1848, 1952, 2086, 2132, 2859, 3154
EcoT14I CCWWGG 2 cut(s) 1887, 2500
EcoT22I ATGCAT 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 1887, 2500
FalI AAGNNNNNCTT 8 cut(s) 495, 527, 735, 767, 1059, 1091, 1986, 2018
FaqI GGGAC 3 cut(s) 1147, 1787, 2104
FauI CCCGC 2 cut(s) 1570, 2879
FauNDI CATATG 2 cut(s) 800, 991
FbaI TGATCA 3 cut(s) 1471, 2208, 2473
FblI GTMKAC 1 cut(s) 1592
FokI GGATG 9 cut(s) 419, 428, 1376, 1400, 1769, 2165, 2309, 2856, 3059
FriOI GRGCYC 1 cut(s) 34
FspBI CTAG 8 cut(s) 39, 641, 918, 1295, 1419, 2717, 2738, 3162
GlaI GCGC 4 cut(s) 1409, 2075, 2147, 2372
GsaI CCCAGC 2 cut(s) 63, 1134
GsuI CTGGAG 5 cut(s) 536, 962, 2527, 2806, 3074
HaeIII GGCC 4 cut(s) 767, 1952, 2137, 2654
HapII CCGG 6 cut(s) 448, 1328, 1588, 1805, 1882, 2419
HgaI GACGC 1 cut(s) 170
HhaI GCGC 4 cut(s) 1410, 2076, 2148, 2373
Hin1I GRCGYC 1 cut(s) 708
Hin6I GCGC 4 cut(s) 1408, 2074, 2146, 2371
HinP1I GCGC 4 cut(s) 1408, 2074, 2146, 2371
HincII GTYRAC 4 cut(s) 796, 1111, 1636, 2695
HindII GTYRAC 4 cut(s) 796, 1111, 1636, 2695
HindIII AAGCTT 2 cut(s) 359, 509
HpaI GTTAAC 1 cut(s) 1636
HpaII CCGG 6 cut(s) 448, 1328, 1588, 1805, 1882, 2419
HphI GGTGA 8 cut(s) 404, 464, 703, 1574, 1999, 2203, 2434, 2596
Hpy166II GTNNAC 6 cut(s) 796, 1111, 1593, 1636, 1811, 2695
Hpy8I GTNNAC 6 cut(s) 796, 1111, 1593, 1636, 1811, 2695
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 5 cut(s) 497, 1545, 1825, 2194, 2228
HpyCH4III ACNGT 7 cut(s) 137, 650, 1060, 1306, 1568, 1747, 2448
HpyCH4IV ACGT 3 cut(s) 100, 708, 2271
HpySE526I ACGT 3 cut(s) 100, 708, 2271
Hsp92I GRCGYC 1 cut(s) 708
HspAI GCGC 4 cut(s) 1408, 2074, 2146, 2371
Kpn2I TCCGGA 1 cut(s) 1881
Ksp22I TGATCA 3 cut(s) 1471, 2208, 2473
KspAI GTTAAC 1 cut(s) 1636
LguI GCTCTTC 1 cut(s) 1485
LmnI GCTCC 4 cut(s) 1330, 1710, 2368, 2717
Lsp1109I GCAGC 9 cut(s) 619, 841, 1006, 1057, 1278, 1516, 1917, 2534, 3195
LweI GCATC 3 cut(s) 406, 955, 1018
MaeI CTAG 8 cut(s) 39, 641, 918, 1295, 1419, 2717, 2738, 3162
MaeII ACGT 3 cut(s) 100, 708, 2271
MaeIII GTNAC 8 cut(s) 203, 709, 1312, 1562, 1695, 1912, 1987, 2970
MflI RGATCY 5 cut(s) 152, 285, 730, 2052, 2793
MhlI GDGCHC 2 cut(s) 34, 1349
MlsI TGGCCA 2 cut(s) 1952, 2654
MluCI AATT 5 cut(s) 261, 1151, 1280, 1655, 2276
MluNI TGGCCA 2 cut(s) 1952, 2654
MmeI TCCRAC 1 cut(s) 457
Mox20I TGGCCA 2 cut(s) 1952, 2654
Mph1103I ATGCAT 1 cut(s) 378
MroI TCCGGA 1 cut(s) 1881
MroXI GAANNNNTTC 3 cut(s) 463, 2021, 2872
MscI TGGCCA 2 cut(s) 1952, 2654
MslI CAYNNNNRTG 2 cut(s) 620, 2109
Msp20I TGGCCA 2 cut(s) 1952, 2654
MspA1I CMGCKG 2 cut(s) 1610, 2547
MspI CCGG 6 cut(s) 448, 1328, 1588, 1805, 1882, 2419
Mva1269I GAATGC 3 cut(s) 547, 1099, 2525
MvaI CCWGG 9 cut(s) 949, 1511, 1667, 1850, 1954, 2088, 2134, 2861, 3156
MvnI CGCG 4 cut(s) 1408, 2148, 2373, 3151
NciI CCSGG 5 cut(s) 448, 449, 1805, 1806, 2420
NcoI CCATGG 1 cut(s) 2500
NdeI CATATG 2 cut(s) 800, 991
NheI GCTAGC 1 cut(s) 1294
NlaIV GGNNCC 2 cut(s) 1719, 2054
NmuCI GTSAC 5 cut(s) 709, 1312, 1562, 1987, 2970
NruI TCGCGA 1 cut(s) 3151
NsiI ATGCAT 1 cut(s) 378
NspI RCATGY 4 cut(s) 380, 1645, 2180, 3199
PaeI GCATGC 1 cut(s) 2180
PagI TCATGA 2 cut(s) 615, 1825
PceI AGGCCT 1 cut(s) 2137
PciSI GCTCTTC 1 cut(s) 1485
PcsI WCGNNNNNNNCGW 1 cut(s) 433
PctI GAATGC 3 cut(s) 547, 1099, 2525
PdmI GAANNNNTTC 3 cut(s) 463, 2021, 2872
PfeI GAWTC 7 cut(s) 464, 1892, 2083, 2611, 2776, 2873, 2879
PflMI CCANNNNNTGG 3 cut(s) 59, 1243, 2245
PmaCI CACGTG 1 cut(s) 101
PmlI CACGTG 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 951
Psp6I CCWGG 9 cut(s) 947, 1509, 1665, 1848, 1952, 2086, 2132, 2859, 3154
PspCI CACGTG 1 cut(s) 101
PspEI GGTNACC 1 cut(s) 1987
PspFI CCCAGC 2 cut(s) 59, 1130
PspGI CCWGG 9 cut(s) 947, 1509, 1665, 1848, 1952, 2086, 2132, 2859, 3154
PspN4I GGNNCC 2 cut(s) 1719, 2054
PspPI GGNCC 4 cut(s) 951, 1422, 1884, 2461
PspPPI RGGWCCY 1 cut(s) 951
PstNI CAGNNNCTG 1 cut(s) 2882
PsuI RGATCY 5 cut(s) 152, 285, 730, 2052, 2793
PvuII CAGCTG 2 cut(s) 1610, 2547
RruI TCGCGA 1 cut(s) 3151
RsaI GTAC 2 cut(s) 1810, 2759
RsaNI GTAC 2 cut(s) 1809, 2758
RseI CAYNNNNRTG 2 cut(s) 620, 2109
SapI GCTCTTC 1 cut(s) 1485
Sau96I GGNCC 4 cut(s) 951, 1422, 1884, 2461
ScaI AGTACT 1 cut(s) 2759
SduI GDGCHC 2 cut(s) 34, 1349
SfaNI GCATC 3 cut(s) 406, 955, 1018
SfcI CTRYAG 1 cut(s) 646
SinI GGWCC 4 cut(s) 951, 1422, 1884, 2461
SmaI CCCGGG 2 cut(s) 449, 1806
SmiMI CAYNNNNRTG 2 cut(s) 620, 2109
SmlI CTYRAG 6 cut(s) 680, 752, 1004, 1865, 2465, 2615
SmoI CTYRAG 6 cut(s) 680, 752, 1004, 1865, 2465, 2615
SphI GCATGC 1 cut(s) 2180
Sse9I AATT 5 cut(s) 261, 1151, 1280, 1655, 2276
SseBI AGGCCT 1 cut(s) 2137
SsiI CCGC 9 cut(s) 825, 1264, 1406, 1577, 2060, 2510, 2819, 2886, 3165
SspI AATATT 1 cut(s) 229
SspMI CTAG 8 cut(s) 39, 641, 918, 1295, 1419, 2717, 2738, 3162
StuI AGGCCT 1 cut(s) 2137
StyI CCWWGG 2 cut(s) 1887, 2500
TaaI ACNGT 7 cut(s) 137, 650, 1060, 1306, 1568, 1747, 2448
TaiI ACGT 3 cut(s) 103, 711, 2274
TaqI TCGA 1 cut(s) 2097
TaqII GACCGA 2 cut(s) 1133, 1219
TasI AATT 5 cut(s) 261, 1151, 1280, 1655, 2276
TatI WGTACW 1 cut(s) 2757
TauI GCSGC 1 cut(s) 1266
TfiI GAWTC 7 cut(s) 464, 1892, 2083, 2611, 2776, 2873, 2879
TscAI CASTG 9 cut(s) 128, 142, 655, 742, 775, 1063, 1432, 1752, 2979
TseFI GTSAC 5 cut(s) 709, 1312, 1562, 1987, 2970
TseI GCWGC 9 cut(s) 632, 854, 1019, 1070, 1266, 1504, 1905, 2547, 3183
Tsp45I GTSAC 5 cut(s) 709, 1312, 1562, 1987, 2970
TspGWI ACGGA 5 cut(s) 425, 1513, 1775, 2045, 2880
TspMI CCCGGG 2 cut(s) 447, 1804
TspRI CASTG 9 cut(s) 128, 142, 655, 742, 775, 1063, 1432, 1752, 2979
Van91I CCANNNNNTGG 3 cut(s) 59, 1243, 2245
VpaK11BI GGWCC 4 cut(s) 951, 1422, 1884, 2461
XagI CCTNNNNNAGG 1 cut(s) 1868
XapI RAATTY 2 cut(s) 1151, 1280
XceI RCATGY 4 cut(s) 380, 1645, 2180, 3199
XmaI CCCGGG 2 cut(s) 447, 1804
XmiI GTMKAC 1 cut(s) 1592
XmnI GAANNNNTTC 3 cut(s) 463, 2021, 2872
XspI CTAG 8 cut(s) 39, 641, 918, 1295, 1419, 2717, 2738, 3162
ZraI GACGTC 1 cut(s) 709
ZrmI AGTACT 1 cut(s) 2759
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.