RLG00000025174

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001181
Physical Location & Seq
Forward (+)
42733612 .. 42740142
6531 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025174

Sequence Viewer

Length: 3780 bp
ATGATCTCCTTAAGCTCTCCATCCTTAACGTCTCAGGCTGTGTTCAGGGAAGGCATCATGACACCACCCTTAGATAATGTTTATTGCTTTTCTATACTTGGGACTTTGACTGACGACCTTGTCCCACCTATTGCCAATCTTCTTAAAACAGTGCCTAATTTGATTAGTTTGCGCATAAGTAATGCAGTCATCCCTCTCAACTCTAGTACTAATTCATCTGGGTTCAATATCAGCTATTGGAAATCCCTGAACCTGGCTTTTGTTGATCATCTTGAGTACGTATCAATACCAATTTTCAACGGAGATAATGAGGTGGAGTTTGCAGCTTATTTGCTTGAAAATGCTAGGAATTTGAAGAGAATGGCTGATGTTCTAGTTGATCGCAAAGTCCAGAATGCTGAAAAGAAGAAGCTATCCTTAGAACTCGCAGGAATTCTGAAGGTTTTGAAGAGGAATCGGGGGATTTGGGGACGCCGCCAGGGCCGAAAGCATAACCCTAACCCTAATTCGCATTGCGGGTGCCGCGAAACTTCACCGGAAGGCAACCGCCGGAGCCACGGAAAGAGTCAGTTCGTAATGGCTGATAATCGAAATGGAAATAGTAAGCCTACAAAGGCAGCTGGAGCAGGAAATAATCCCTATTCCATTGATTTGAATGTCTTCAGTCAGAGATTGAAGTCCCTGTATTCGCACTGGAATGAGCAGCCTGAGTTGTGGGATTCTAGTGACGTTATTGCTATTGCAACACCGCCGCAATCAGATGAAATCCGGTACCTTAAGTCTTCAGCATTGCAGATGTGGTTGGTTGGGTATGATGTTCCGGATACGATAATGGTGTTCATGAAGAAGCAGATTCATTTCTTGGGTAGCCAGAAGAAGGTGTCTTTTTTCCAAGGTGTGAAGAAGTCTGCCAAAGAGGCTGTGGGTGTTGATGTTGTGACGCATGTGAAGGCAAAGAGTGATGATGGCAGTGCTCTGATGGATACTATTTTGGGTGTAATTTGTGCGCAACCAAAGGCTGATGGCCGTGATACTGTTGTTGTAGGAAGGATAGCAAGGGAGGATCCTGAGGGGAATCTTTTGAAATTGTGGTTTGAGAAGCTGAAGGGTGCAACTGTTCAGCTTGGTGATGTGACAAATGGGTTGTCTGACTTGTTTGCTGTCAAGGAGAAAGAGGAACTTGGTAATGTCAAGAAAGCCGCTTACTTAAATACTCTTGTTATGCAGAAAATGGTGTCGAAGCTTGAGAGTGTTATTGATGATGAGAAGAAGGTCACTCACTCTTCTTTGATGGATGAGACAGAGAAACTTATACTGGAACCCTCTAAAGTTGGGCTGAAGCTCAATCCTCAGAATGTTGATATCTGTTACCCTCCAATCTTTCAGAGTGGAGGAAAGTATGATCTCAGACCTGGTGCTGCCAGCAATGATGAGCTTCTTTATTATGATTCTGCAAGTGTGATCATATGTGCTATTGGATCCCGTTATCACAATTACTGCTCGAATGCTGCCAGGACTTTTTTGATTGATGCTAACCCCCTTCAGAGTAAGGCATATGAGGTTCTTCTCAAGGCCCATGCTGCTGCCATTAGTGAGTTGAAGCCTGACAATAAAGCCAGTGCTGCATATCTAGCAGCACTGGCTGTTGTAGAGAAGGATGCTCCTGAACTATTACCTAATCTGACAAAATCTACGGGGACAGGCATTGGTATTGAGTTCCGTGAATCTGGGTTGAATCTTAATGCAAAAAATGACCGGGTGGTGAAAGCTGGCATGGTCTTTAATGTGTCACTTGGTTTTCATAACTTGCAGAGTGGTATCAGTGATCCAAAGCACCAGAACTTCTCTTTGTTGCTAGCTGACACTGTTGTTGTCAACAATGACAAACCAGAGGTGCTGACAGTAAAAGTTTCTAAAGCTGTTAAAGATGTGGCATACTCCTTTAATGAAGTTGAAGAAGAAAAGCCTGAGAAAGGAAAAGGTCCGGCTAATGGAACAGAGGCCTTGATATCCAAAACCAATCTGAGGTCAGACAATAATGAGAAATCAAAGGAGGAGCTACGAAGGCATCACCAGGCAGAACTTGCCCGGCAGAAAAATGAAGAAACAGCCCGTAGACTTGCTGGTGATGGACATGGATCAGGTAATAATCGTTCCACTGCAAAGTCTTCATCCGATTTAGTGGCCTACAAGAATGTAAATGATCTCAATCCTAAAAGAGATCTGATGATTCAAGTTGACCAGAATAATGAAGCTGTTCTTTTGCCTATATATGGGAGCATGGTCCCCTTTCATGTTGCCACCATAAGGACAGTTAGTGTCCAAGCGGAGTCAGCCAACACTAATCAGGCTTGTTATATCAGAATCATCTTCAATGTTCCTGGGACTCCTTTTAGTCCTCATGATACAAACTCGCTGAAAAACTTGGGTTCTATATATTTAAAGGAAGTTTCATTTCGTTCCAAGGACACAAGGCACATAAATGAAGTTACACAGGTGATCAAAAACCTTCGGAAGCGAGTTGTGTCCAGGGAGAATGAGAGAGCTGAGAGGGCTACACTTGTTACTCAGGAGAAACTCCAACTAGCTGGTAACAAATTTAAGCCGCTGAGATTGAATGACCTCTGGATTCGACCTGTGTTTGGTGGACGTGGCAGAAAGATTCCGGGTACTCTTGAAGCTCATGCAAATGGGTTTCGTTACTCTACCACCAGACAAGATGAGCGCGTGGATGTAATGTATGGAAATATTAAGCATGCTTTTTTCCAGCCAGCAGAGAATGAGATGATTACTCTCCTTCATTTTCATCTTCACAATCACATAATGGTGGGCAACAAGAAAACCAAAGACGTGCAATTCTATGTGGAGGTTATGGATGTAGTTCAGACCCTGGGAAGTAGTAAGAGATCTGCCTACGACCCAGATGAGATTGAAGAAGAGCAACGAGAGAGGGCTAGGAAGAACAAAGTCAACACTGAATTTCGGACCTTTGTGACTCGGGTACAGGATCTTTGGCAGCAGCCCCAGTTTGAAGGCCTTGAACTTATGTTTGATCAGCCTATGAGGGAGCTTGGTTTCCACGGTGTGCCTTATAAATCCTCAGCATATATCGTCCCTACTACAAATTGCCTGGTTGAACTGATTGAAACTCCTTTTCTTGTTGTCACTCTTGGCGAGATTGAAATTGTGAATCTGGAAAGGGTTGGGCTTGGACAGAAGAATTTTGACCTGGCAATTGTATTCAAGGATTTTAAAAAAGATGTTCTTCGGATTGATTCGATCCCATCCACATCACTTGAAGGTCTCAAGGAATGGCTTGACGACACAGAGATCAAGTACTATGAGAGCAGGATGAATCTGTCGTGGAAACCTATACTGAAGACAATTACTGAGGATCCACAGAGCTTCCTTGATAATGGAGGTTGGGAGTTTTTGAATTTGGAGGGAACGGATTCAGACTCTGAACAGTCTCAGGAGTCAGACCAGGGTTATGAACCATCTGATATGGAATCTGAATCAGAATCTGAGGATGATTCTTCTGATAGCGCGTCACTTGTGGAGTCTGAGGATGATGAGGAGGAAGATTCAGAAGAAGTCTCGGAGGATGAGGAGGGGAAGACATGGGAAGAGTTGGAGAGGGAAGCAAGCAATGCAGACAGGGAGAAAGGCAACGAGTCGGACAGCGAGGAGGACAGGAAGAGAAGGAAGATGAAAGCTTTTGGCAAGGCTCGAGCCCCGCCTGCTGGTAGCATCCCAAAGCGGTCCAAGCTGAGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1260

Amino Acids

140.92

Weight (kDa)

5.95

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FACT-Spt16_Nlob PF14826 216 - 380 1.8e-40 FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24 PF00557 397 - 626 9.6e-29 Metallopeptidase family M24
SPT16 PF08644 744 - 860 4.4e-35 FACT complex subunit (SPT16/CDC68)
PH_SPT16 PF24824 871 - 1001 9.9e-55 FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle PF08512 1028 - 1115 2.3e-22 Histone chaperone Rttp106-like, middle domain
SPT16_C PF21091 1155 - 1236 5.8e-17 FACT complex subunit SPT16, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3093
AasI GACNNNNNNGTC 1 cut(s) 119
Acc16I TGCGCA 2 cut(s) 173, 1008
Acc65I GGTACC 1 cut(s) 771
AccB1I GGYRCC 2 cut(s) 519, 771
AccI GTMKAC 1 cut(s) 2116
AccII CGCG 3 cut(s) 525, 2727, 3548
AccIII TCCGGA 1 cut(s) 820
AcoI YGGCCR 1 cut(s) 1024
AcsI RAATTY 6 cut(s) 349, 432, 2597, 2978, 3220, 3436
AcuI CTGAAG 7 cut(s) 458, 646, 768, 1124, 1358, 1526, 3398
AcyI GRCGYC 1 cut(s) 472
AdeI CACNNNGTG 1 cut(s) 3085
AfaI GTAC 6 cut(s) 208, 278, 773, 2671, 3003, 3338
AflII CTTAAG 2 cut(s) 10, 776
AjiI CACGTC 2 cut(s) 2651, 2851
Alw21I GWGCWC 1 cut(s) 976
Alw26I GTCTC 5 cut(s) 36, 1292, 3308, 3474, 3601
AlwNI CAGNNNCTG 3 cut(s) 2890, 3461, 3524
Ama87I CYCGRG 2 cut(s) 2997, 3729
Aor13HI TCCGGA 1 cut(s) 820
AoxI GGCC 6 cut(s) 481, 1024, 1572, 2001, 2184, 3034
ApoI RAATTY 6 cut(s) 349, 432, 2597, 2978, 3220, 3436
ArsI GACNNNNNNTTYG 2 cut(s) 1130, 1162
Asp700I GAANNNNTTC 3 cut(s) 659, 2256, 3451
Asp718I GGTACC 1 cut(s) 771
AspLEI GCGC 4 cut(s) 174, 1009, 2727, 3548
AspS9I GGNCC 6 cut(s) 481, 1573, 1982, 2284, 2985, 3762
AsuC2I CCSGG 3 cut(s) 1757, 2089, 2667
AsuHPI GGTGA 6 cut(s) 525, 1139, 1774, 2063, 2138, 2509
AsuNHI GCTAGC 1 cut(s) 1855
AvaI CYCGRG 2 cut(s) 2997, 3729
AvaII GGWCC 4 cut(s) 1982, 2284, 2985, 3762
AxyI CCTNAGG 1 cut(s) 1068
BaeI ACNNNNGTAYC 2 cut(s) 1023, 1056
BamHI GGATCC 3 cut(s) 1063, 1478, 3394
BanI GGYRCC 2 cut(s) 519, 771
BanII GRGCYC 1 cut(s) 3736
BbsI GAAGAC 5 cut(s) 652, 774, 2160, 3386, 3623
Bbv12I GWGCWC 1 cut(s) 976
BbvCI CCTCAGC 2 cut(s) 3100, 3770
BccI CCATC 8 cut(s) 28, 959, 973, 1016, 1285, 2123, 3292, 3505
BceAI ACGGC 1 cut(s) 1011
BciVI GTATCC 2 cut(s) 817, 976
BclI TGATCA 4 cut(s) 265, 1461, 2499, 3052
BcnI CCSGG 3 cut(s) 1757, 2089, 2667
BcoDI GTCTC 5 cut(s) 36, 1292, 3308, 3474, 3601
BfaI CTAG 8 cut(s) 204, 345, 374, 723, 1631, 1856, 2585, 2955
BfrI CTTAAG 2 cut(s) 10, 776
BfuI GTATCC 2 cut(s) 817, 976
BglI GCCNNNNNGGC 2 cut(s) 480, 917
BglII AGATCT 2 cut(s) 2221, 2906
BmcAI AGTACT 2 cut(s) 208, 3338
Bme18I GGWCC 4 cut(s) 1982, 2284, 2985, 3762
BmeT110I CYCGRG 2 cut(s) 2997, 3729
BmgBI CACGTC 2 cut(s) 2651, 2851
BmgT120I GGNCC 6 cut(s) 481, 1573, 1982, 2284, 2985, 3762
BmiI GGNNCC 8 cut(s) 521, 554, 773, 1065, 1320, 1480, 2286, 3396
BmrI ACTGGG 1 cut(s) 3019
BmsI GCATC 5 cut(s) 63, 1519, 1648, 2077, 3759
BmtI GCTAGC 1 cut(s) 1859
BmuI ACTGGG 1 cut(s) 3019
BpiI GAAGAC 5 cut(s) 652, 774, 2160, 3386, 3623
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 2 cut(s) 3100, 3770
BpuEI CTTGAG 4 cut(s) 293, 1265, 1553, 3290
BpuMI CCSGG 3 cut(s) 1757, 2089, 2667
BsaAI YACGTR 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 2208
BsaHI GRCGYC 1 cut(s) 472
BsaI GGTCTC 1 cut(s) 3308
BsaWI WCCGGW 3 cut(s) 535, 768, 820
Bse1I ACTGG 5 cut(s) 698, 1320, 1617, 1644, 3025
Bse21I CCTNAGG 1 cut(s) 1068
Bse3DI GCAATG 4 cut(s) 511, 788, 1432, 3655
Bse8I GATNNNNATC 1 cut(s) 2208
BseAI TCCGGA 1 cut(s) 820
BseJI GATNNNNATC 1 cut(s) 2208
BseMI GCAATG 4 cut(s) 511, 788, 1432, 3655
BseNI ACTGG 5 cut(s) 698, 1320, 1617, 1644, 3025
BseRI GAGGAG 4 cut(s) 2069, 3590, 3623, 3701
Bsh1236I CGCG 3 cut(s) 525, 2727, 3548
BshFI GGCC 6 cut(s) 483, 1026, 1574, 2003, 2186, 3036
BshNI GGYRCC 2 cut(s) 519, 771
BsiHKAI GWGCWC 1 cut(s) 976
BsiHKCI CYCGRG 2 cut(s) 2997, 3729
BsiSI CCGG 8 cut(s) 536, 550, 769, 821, 1756, 1985, 2089, 2666
BslFI GGGAC 8 cut(s) 107, 115, 483, 664, 1711, 2270, 2398, 3098
BsmAI GTCTC 5 cut(s) 36, 1292, 3308, 3474, 3601
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 8 cut(s) 107, 115, 483, 664, 1711, 2270, 2398, 3098
BsmI GAATGC 2 cut(s) 400, 1510
BsnI GGCC 6 cut(s) 483, 1026, 1574, 2003, 2186, 3036
Bso31I GGTCTC 1 cut(s) 3308
BsoBI CYCGRG 2 cut(s) 2997, 3729
Bsp1286I GDGCHC 2 cut(s) 976, 3736
Bsp13I TCCGGA 1 cut(s) 820
BspANI GGCC 6 cut(s) 483, 1026, 1574, 2003, 2186, 3036
BspEI TCCGGA 1 cut(s) 820
BspFNI CGCG 3 cut(s) 525, 2727, 3548
BspHI TCATGA 3 cut(s) 57, 840, 2401
BspLI GGNNCC 8 cut(s) 521, 554, 773, 1065, 1320, 1480, 2286, 3396
BspOI GCTAGC 1 cut(s) 1859
BspQI GCTCTTC 1 cut(s) 2931
BspT107I GGYRCC 2 cut(s) 519, 771
BspTI CTTAAG 2 cut(s) 10, 776
BspTNI GGTCTC 1 cut(s) 3308
BsrDI GCAATG 4 cut(s) 511, 788, 1432, 3655
BsrI ACTGG 5 cut(s) 698, 1320, 1617, 1644, 3025
BssNI GRCGYC 1 cut(s) 472
BssT1I CCWWGG 2 cut(s) 892, 2463
Bst4CI ACNGT 8 cut(s) 151, 1036, 1117, 1867, 1903, 2314, 3083, 3468
Bst6I CTCTTC 6 cut(s) 350, 443, 1288, 2931, 3621, 3692
BstACI GRCGYC 1 cut(s) 472
BstAFI CTTAAG 2 cut(s) 10, 776
BstAPI GCANNNNNTGC 2 cut(s) 2084, 3650
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 7 cut(s) 1423, 1771, 1857, 2757, 2772, 3646, 3741
BstDSI CCRYGG 2 cut(s) 556, 3079
BstENI CCTNNNNNAGG 1 cut(s) 1971
BstFNI CGCG 3 cut(s) 525, 2727, 3548
BstHHI GCGC 4 cut(s) 174, 1009, 2727, 3548
BstMAI GTCTC 5 cut(s) 36, 1292, 3308, 3474, 3601
BstNSI RCATGY 2 cut(s) 947, 2759
BstSNI TACGTA 1 cut(s) 280
BstUI CGCG 3 cut(s) 525, 2727, 3548
BstV2I GAAGAC 5 cut(s) 652, 774, 2160, 3386, 3623
BstX2I RGATCY 6 cut(s) 1063, 1478, 2221, 2906, 3007, 3394
BstXI CCANNNNNNTGG 1 cut(s) 2588
BstYI RGATCY 6 cut(s) 1063, 1478, 2221, 2906, 3007, 3394
Bsu36I CCTNAGG 1 cut(s) 1068
BsuI GTATCC 2 cut(s) 817, 976
BsuRI GGCC 6 cut(s) 483, 1026, 1574, 2003, 2186, 3036
BtgI CCRYGG 2 cut(s) 556, 3079
BtrI CACGTC 2 cut(s) 2651, 2851
BtsI GCAGTG 2 cut(s) 976, 2157
BtsIMutI CAGTG 9 cut(s) 156, 691, 976, 1624, 1637, 1828, 1863, 2157, 2973
Cac8I GCNNGC 7 cut(s) 1423, 1771, 1857, 2757, 2772, 3646, 3741
CaiI CAGNNNCTG 3 cut(s) 2890, 3461, 3524
CciI TCATGA 3 cut(s) 57, 840, 2401
CfoI GCGC 4 cut(s) 174, 1009, 2727, 3548
Cfr13I GGNCC 6 cut(s) 481, 1573, 1982, 2284, 2985, 3762
CseI GACGC 3 cut(s) 480, 949, 3537
CsiI ACCWGGT 1 cut(s) 1411
Csp6I GTAC 6 cut(s) 207, 277, 772, 2670, 3002, 3337
CspCI CAANNNNNGTGG 2 cut(s) 3277, 3312
CviQI GTAC 6 cut(s) 207, 277, 772, 2670, 3002, 3337
DraI TTTAAA 2 cut(s) 2442, 3253
DraIII CACNNNGTG 1 cut(s) 3085
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 1024
Eam1104I CTCTTC 6 cut(s) 350, 443, 1288, 2931, 3621, 3692
EarI CTCTTC 6 cut(s) 350, 443, 1288, 2931, 3621, 3692
Eco105I TACGTA 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 892, 2463
Eco147I AGGCCT 2 cut(s) 2003, 3036
Eco24I GRGCYC 1 cut(s) 3736
Eco31I GGTCTC 1 cut(s) 3308
Eco32I GATATC 2 cut(s) 1363, 2010
Eco47I GGWCC 4 cut(s) 1982, 2284, 2985, 3762
Eco57I CTGAAG 7 cut(s) 458, 646, 768, 1124, 1358, 1526, 3398
Eco81I CCTNAGG 1 cut(s) 1068
Eco88I CYCGRG 2 cut(s) 2997, 3729
EcoNI CCTNNNNNAGG 1 cut(s) 1971
EcoRI GAATTC 1 cut(s) 432
EcoRV GATATC 2 cut(s) 1363, 2010
EcoT14I CCWWGG 2 cut(s) 892, 2463
EcoT38I GRGCYC 1 cut(s) 3736
ErhI CCWWGG 2 cut(s) 892, 2463
Esp3I CGTCTC 1 cut(s) 36
FalI AAGNNNNNCTT 8 cut(s) 401, 433, 1164, 1196, 2244, 2276, 3249, 3281
FaqI GGGAC 8 cut(s) 107, 115, 483, 664, 1711, 2270, 2398, 3098
FauI CCCGC 2 cut(s) 509, 3744
FauNDI CATATG 2 cut(s) 1466, 1555
FbaI TGATCA 4 cut(s) 265, 1461, 2499, 3052
FblI GTMKAC 1 cut(s) 2116
FriOI GRGCYC 1 cut(s) 3736
FspBI CTAG 8 cut(s) 204, 345, 374, 723, 1631, 1856, 2585, 2955
FspI TGCGCA 2 cut(s) 173, 1008
GlaI GCGC 4 cut(s) 173, 1008, 2726, 3547
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 6 cut(s) 483, 1026, 1574, 2003, 2186, 3036
HapII CCGG 8 cut(s) 536, 550, 769, 821, 1756, 1985, 2089, 2666
HgaI GACGC 3 cut(s) 480, 949, 3537
HhaI GCGC 4 cut(s) 174, 1009, 2727, 3548
Hin1I GRCGYC 1 cut(s) 472
Hin6I GCGC 4 cut(s) 172, 1007, 2725, 3546
HinP1I GCGC 4 cut(s) 172, 1007, 2725, 3546
HincII GTYRAC 3 cut(s) 1876, 2239, 2971
HindII GTYRAC 3 cut(s) 1876, 2239, 2971
HindIII AAGCTT 2 cut(s) 1241, 3714
HpaII CCGG 8 cut(s) 536, 550, 769, 821, 1756, 1985, 2089, 2666
HphI GGTGA 6 cut(s) 525, 1139, 1774, 2063, 2138, 2509
Hpy166II GTNNAC 5 cut(s) 1876, 2117, 2239, 2648, 2971
Hpy8I GTNNAC 5 cut(s) 1876, 2117, 2239, 2648, 2971
HpyCH4III ACNGT 8 cut(s) 151, 1036, 1117, 1867, 1903, 2314, 3083, 3468
HpyCH4IV ACGT 5 cut(s) 29, 279, 729, 2650, 2850
HpySE526I ACGT 5 cut(s) 29, 279, 729, 2650, 2850
Hsp92I GRCGYC 1 cut(s) 472
HspAI GCGC 4 cut(s) 172, 1007, 2725, 3546
Kpn2I TCCGGA 1 cut(s) 820
KpnI GGTACC 1 cut(s) 775
Ksp22I TGATCA 4 cut(s) 265, 1461, 2499, 3052
LguI GCTCTTC 1 cut(s) 2931
LmnI GCTCC 6 cut(s) 552, 623, 1666, 2056, 2277, 3067
LweI GCATC 5 cut(s) 63, 1519, 1648, 2077, 3759
MabI ACCWGGT 1 cut(s) 1411
MaeI CTAG 8 cut(s) 204, 345, 374, 723, 1631, 1856, 2585, 2955
MaeII ACGT 5 cut(s) 29, 279, 729, 2650, 2850
MfeI CAATTG 1 cut(s) 3234
MflI RGATCY 6 cut(s) 1063, 1478, 2221, 2906, 3007, 3394
MhlI GDGCHC 2 cut(s) 976, 3736
MlyI GAGTC 8 cut(s) 574, 2339, 2380, 2989, 3452, 3485, 3569, 3683
MmeI TCCRAC 3 cut(s) 2605, 3612, 3657
MroI TCCGGA 1 cut(s) 820
MroXI GAANNNNTTC 3 cut(s) 659, 2256, 3451
MslI CAYNNNNRTG 4 cut(s) 696, 2481, 2688, 2825
MspA1I CMGCKG 2 cut(s) 620, 2608
MspCI CTTAAG 2 cut(s) 10, 776
MspI CCGG 8 cut(s) 536, 550, 769, 821, 1756, 1985, 2089, 2666
MunI CAATTG 1 cut(s) 3234
Mva1269I GAATGC 2 cut(s) 400, 1510
MvnI CGCG 3 cut(s) 525, 2727, 3548
NciI CCSGG 3 cut(s) 1757, 2089, 2667
NdeI CATATG 2 cut(s) 1466, 1555
NheI GCTAGC 1 cut(s) 1855
NlaIV GGNNCC 8 cut(s) 521, 554, 773, 1065, 1320, 1480, 2286, 3396
NmuCI GTSAC 8 cut(s) 725, 937, 1132, 1273, 1788, 2992, 3163, 3549
NsbI TGCGCA 2 cut(s) 173, 1008
NspI RCATGY 2 cut(s) 947, 2759
PaeI GCATGC 1 cut(s) 2759
PaeR7I CTCGAG 1 cut(s) 3729
PagI TCATGA 3 cut(s) 57, 840, 2401
PasI CCCWGGG 1 cut(s) 2890
PceI AGGCCT 2 cut(s) 2003, 3036
PciSI GCTCTTC 1 cut(s) 2931
PctI GAATGC 2 cut(s) 400, 1510
PdmI GAANNNNTTC 3 cut(s) 659, 2256, 3451
PflFI GACNNNGTC 1 cut(s) 119
PleI GAGTC 8 cut(s) 573, 2338, 2380, 2989, 3452, 3484, 3568, 3682
PpsI GAGTC 8 cut(s) 573, 2338, 2380, 2989, 3452, 3484, 3568, 3682
Ppu21I YACGTR 1 cut(s) 280
PsiI TTATAA 1 cut(s) 3093
PspN4I GGNNCC 8 cut(s) 521, 554, 773, 1065, 1320, 1480, 2286, 3396
PspPI GGNCC 6 cut(s) 481, 1573, 1982, 2284, 2985, 3762
PspXI VCTCGAGB 1 cut(s) 3729
PsrI GAACNNNNNNTAC 2 cut(s) 2137, 2169
PstNI CAGNNNCTG 3 cut(s) 2890, 3461, 3524
PsuI RGATCY 6 cut(s) 1063, 1478, 2221, 2906, 3007, 3394
PsyI GACNNNGTC 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 6 cut(s) 208, 278, 773, 2671, 3003, 3338
RsaNI GTAC 6 cut(s) 207, 277, 772, 2670, 3002, 3337
RseI CAYNNNNRTG 4 cut(s) 696, 2481, 2688, 2825
SapI GCTCTTC 1 cut(s) 2931
Sau96I GGNCC 6 cut(s) 481, 1573, 1982, 2284, 2985, 3762
ScaI AGTACT 2 cut(s) 208, 3338
SchI GAGTC 8 cut(s) 574, 2339, 2380, 2989, 3452, 3485, 3569, 3683
SduI GDGCHC 2 cut(s) 976, 3736
SexAI ACCWGGT 1 cut(s) 1411
SfaNI GCATC 5 cut(s) 63, 1519, 1648, 2077, 3759
Sfr274I CTCGAG 1 cut(s) 3729
SinI GGWCC 4 cut(s) 1982, 2284, 2985, 3762
SlaI CTCGAG 1 cut(s) 3729
SmiMI CAYNNNNRTG 4 cut(s) 696, 2481, 2688, 2825
SmlI CTYRAG 7 cut(s) 10, 272, 776, 1244, 1568, 3305, 3729
SmoI CTYRAG 7 cut(s) 10, 272, 776, 1244, 1568, 3305, 3729
SnaBI TACGTA 1 cut(s) 280
SphI GCATGC 1 cut(s) 2759
SseBI AGGCCT 2 cut(s) 2003, 3036
SspI AATATT 1 cut(s) 2749
SspMI CTAG 8 cut(s) 204, 345, 374, 723, 1631, 1856, 2585, 2955
StuI AGGCCT 2 cut(s) 2003, 3036
StyI CCWWGG 2 cut(s) 892, 2463
TaaI ACNGT 8 cut(s) 151, 1036, 1117, 1867, 1903, 2314, 3083, 3468
TaiI ACGT 5 cut(s) 32, 282, 732, 2653, 2853
TaqI TCGA 6 cut(s) 589, 1238, 1502, 2632, 3278, 3730
TatI WGTACW 2 cut(s) 206, 3336
TauI GCSGC 5 cut(s) 477, 525, 754, 1202, 2608
TscAI CASTG 9 cut(s) 156, 698, 976, 1624, 1644, 1828, 1870, 2164, 2980
TseFI GTSAC 8 cut(s) 725, 937, 1132, 1273, 1788, 2992, 3163, 3549
Tsp45I GTSAC 8 cut(s) 725, 937, 1132, 1273, 1788, 2992, 3163, 3549
TspGWI ACGGA 4 cut(s) 315, 573, 1709, 3464
TspRI CASTG 9 cut(s) 156, 698, 976, 1624, 1644, 1828, 1870, 2164, 2980
Tth111I GACNNNGTC 1 cut(s) 119
Vha464I CTTAAG 2 cut(s) 10, 776
VpaK11BI GGWCC 4 cut(s) 1982, 2284, 2985, 3762
XagI CCTNNNNNAGG 1 cut(s) 1971
XapI RAATTY 6 cut(s) 349, 432, 2597, 2978, 3220, 3436
XceI RCATGY 2 cut(s) 947, 2759
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XhoI CTCGAG 1 cut(s) 3729
XmiI GTMKAC 1 cut(s) 2116
XmnI GAANNNNTTC 3 cut(s) 659, 2256, 3451
XspI CTAG 8 cut(s) 204, 345, 374, 723, 1631, 1856, 2585, 2955
ZrmI AGTACT 2 cut(s) 208, 3338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.