MD04G1188700.v1.1

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001181
Physical Location & Seq
Forward (+)
27931862 .. 27935677
3816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1188700.v1.1.491

Sequence Viewer

Length: 3213 bp
ATGGCTGATCGTCGAAATGGAAATAGCCAGCCCGCTAATGGGAAGGCTGCAGCTGGAGCAGGAAGTGCCTATAACATTGATTTGAAAGTCTTCAGCGAGAGATTGAAGTTACTTTATTCTCATTGGAATAAACATCGATCTGATTTGTGGGGTTCTTCTGATGTTGTTGCAATAGCGACACCAAAAGCATCAGAAGATCTGCGGTACCTTAAATCTTCAGCATTGAATATTTGGCTGGTGGGTTATGAGTTTCCAGAGACCATTATGGTTTTCATGAAGAAGCAGATTCATTTCTTGTGCAGTACGAAGAAGGTGTCTTTGTTGGAAGTTGTAAAAAAGTCTGCCAAGGAAGCTGTGGGTGTTGATGTTGTGATGCATGAGAAGACAAGGAGTGATGATGGAAGTGCTCTGATGGAGGCAATTTTCCGTGCCATTCGTGCTCAATCGAAGGCTGATGGCCATGACACAGCTGTCGTTGGACATATTGCAAGGGAGGCCCCTGAGGGAAACCTCTTGGAAACATGGTCCAAGAAGCTTGAGGATGCAAATTTTCAGTTGGGCGATGTAACAAATGGGTTGTCTGACCTGTTTGCTGTTAAGGACAATGACGAGATTTTAAATATCAAGAGAGCCGCTGTCTTGTCTGCTAGTGTCATGACCGAAGTAGTTGTTAAAAAGCTTGAGACTGTTATTGATGAGGAGAAGAAAGTTACCCATTCTTCTTTGATGGATGAGGCAGAGAAAGCTATAATGGAACCATCTAAAGCTAAGGTGAAGCTCCGGGCTGATAATGTTGATATCTGTTACCCCCCAATATTTCAGAGTGGAGGACAGTTTGATCTCAGACCCAGTGCTGCTAGCAATGATGAGTTGCTTTATTATGATTCTGCTAGCGCGATCATATGTGCTGTTGGGTCCCGCTACAAGAGTTACTGCTCAAATATTGCCAGGACCTTTCTGATTGATGCTACTTCCAGTCAGAGCAAGGCATATGAAGTTCTCTTCAAGGCCCATAATGCAGCCATTAGTGAGTTGAAGCCTGGGAAGACGGTCAGTGCTGCTTATCAAGCTGCCCTTGCTGTGGTGGAGAAGGAGGCTCCTGAATTAGTATCAAATTTGACAAAATCGGCTGGGACTGGTATCGGCCTTGAGTTCCGTGAATCTGGGTTGAATATAAATGCAAAGAATGAGCGGTTGGTGAAAGCAGGCATGGTCTTTAATGTGTCACTTGGGTTTCAGAACTTACAGAGTGGTCGCAGCAATCCTAAAAACCAAAACTACGCTTTGTTGCTTGCGGACACTGTAGTTATTGGCAATGACAAGCCAGAGGTGGTGACAAGTAGAAGCTCTAAGAATCTTCAGGATGTGGCATACTCCTTTAATGATGGAGATGAAGAGGAAGCTGAAAAGCCCAAAAAACCGAATGATGAGGCTAATGGAAGGGAGGCCTTAATGTCTAAGACAACATTGAGGTCAGACAATCATGAGATTTCAAAGGAGGAACTCCGCAGGCAACATCAGGCAGAGCTTGCCCGTCAGAAAAATGAAGAAACTGCCCGCAGACTTGCTGGTGCTGGATCTGCACCGGGTGACAATCGTTCTGCAGCAAGGGCTTTAACGGATTTAATTGCCTACAAGAATGTAAATGATGTCGCTCCACCAAAAGGTCTGAATCAACTAATGATTCAGATTGACCAGAAGAATGAAGCTGTTCTGTTGCCTATTTATGGCAGTATGATCCCTTTTCATGTTGCAACCATAAGGACAGTTTCCAGTCAACAGGACACAAATCGGAACTGTTATATCAGAATCATGTTTAATGTCCCTGGGACTCCTTTTAGTCCTCATGATGGCAACTCACTGAAGAACCTTGGGTCTATATATTTAAAAGAAGTTTCATTTCGCTCCAAGGATCCAAGGCACATAAGTGAAGTGGTACAGGCGATTAAAGGGCTGCGAAGGCAAGTTGTGTCCAGGGAGTCCTTGATAGCTGAGAAGGCGACACTGGTTACTCAGGAGAAACTCCAAATAGCTGGTAACAGATTTAAGCCAATAAGATTGTCTGACCTTTGGATTCGACCTGTTTTTGGTGGCCGTGGAAGAAAGATTCCTGGTGCTCTAGAAGCTCATGCAAATGGGTTTCGTTATTCTACCACCAGGCAGGAGGAGCGCCTGGATGTTATGTTTGCAAATATTAAGCATGCCTTTTTCCAGCCAGCGGAGAATGAGATGATCACTCTCCTCCACTTTCATCTGCACGATGACATCATGGTGGGCAACAAGAAAACAAAAAACGTGCAATTCTATGTTGAGGTGATGGACACAGTTCAGACCTTGGGAGGTAGTAGGAGGTCTGCTTATGACCCAGATGAGATTGAAGAAGAGCAGCGGGAGAGGGATAGGAAGAACAAAATAAATATGGATTTTCAGAACTTTGTGAATCGGGTTAATGAGCTTTGGGGGCAGTCACAGTTTAGGGACCTTGAGTTTGATCAACCTCTGAGAGAGCTTGGTTTCCATGGTGTACCTCATAAATCATCAGCATTTATTGTCCCTACTTCAACCTGCTTGGTTGAACTGATAGAAACTCCTTTCCTTGTTGTCAGTCTAAGTGAGATTGAGATTGTGAATCTGGAAAGAGTTGGCCTTGGGCAGAAGAATTTTGACATGACCATTGTATTCAAGGACTTCAAAAAGGACGTTCTCCGGATTGACTCGATCCCTTCGACAGCACTTGATGGTATCAAGGAATGGCTCGACACAACTGACCTCAAGTATTATGAGAGCAGGCTGAATCTCAACTGGAAACAGATACTGAAGACAATCACCGATGACCCGCAGAGCTTCATTGAAGATGGAGGTTGGGAGTTTTTGAATTTGGAAGCGTCTGATTCAGAGTCTGAACAGTCGGAGGAGTCAGACCAGGGTTACGAACCATCGGATATCGAATCTGCTGAATCAGATGAATCGGATGATGAGGATTCTGATAGTGAATCGGTTGTTGAGTCTGAGGATGAGGACGGAGAAGCGGAGTCAGATGAGGATGGGTTATCGTGGGATGAACTGGAGAGGGAAGCAAGCAATGCAGATAGGGAGAAAGGGAACGAATCAGACAGTGAGGAGGACAGGAAGAGAAGGAAGGCGAAAGCTTTTGGAAAGTCTCGAGCCCCACCTCCTACTAGTAGCATCCCGAAGCGGAGTAAGATGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1071

Amino Acids

119.5

Weight (kDa)

5.39

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FACT-Spt16_Nlob PF14826 26 - 191 1.2e-48 FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24 PF00557 208 - 437 5.7e-30 Metallopeptidase family M24
SPT16 PF08644 562 - 675 1.6e-33 FACT complex subunit (SPT16/CDC68)
PH_SPT16 PF24824 685 - 816 4.6e-54 FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle PF08512 840 - 928 1.6e-23 Histone chaperone Rttp106-like, middle domain
SPT16_C PF21091 968 - 1047 7.6e-14 FACT complex subunit SPT16, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
Acc36I ACCTGC 1 cut(s) 2573
Acc65I GGTACC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 204
AccBSI CCGCTC 1 cut(s) 1192
AccII CGCG 1 cut(s) 896
AccIII TCCGGA 1 cut(s) 2706
AclWI GGATC 5 cut(s) 1585, 1732, 1907, 1920, 2713
AcoI YGGCCR 2 cut(s) 457, 2092
AcsI RAATTY 4 cut(s) 547, 1114, 2659, 2875
AcuI CTGAAG 5 cut(s) 76, 201, 1343, 1883, 2837
AdeI CACNNNGTG 1 cut(s) 1589
AfaI GTAC 4 cut(s) 206, 304, 1938, 2526
AfiI CCNNNNNNNGG 8 cut(s) 38, 39, 1081, 1664, 1727, 1850, 2087, 2218
AhlI ACTAGT 1 cut(s) 3179
AjnI CCWGG 8 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2922
AjuI GAANNNNNNNTTGG 2 cut(s) 1178, 1210
AleI CACNNNNGTG 1 cut(s) 1926
Alw21I GWGCWC 3 cut(s) 409, 442, 2119
Alw26I GTCTC 3 cut(s) 251, 677, 3165
AlwI GGATC 5 cut(s) 1585, 1732, 1907, 1920, 2713
AlwNI CAGNNNCTG 2 cut(s) 2815, 2900
Ama87I CYCGRG 1 cut(s) 3162
Aor13HI TCCGGA 1 cut(s) 2706
AoxI GGCC 7 cut(s) 457, 495, 1008, 1144, 1446, 2092, 2644
ApoI RAATTY 4 cut(s) 547, 1114, 2659, 2875
ArsI GACNNNNNNTTYG 6 cut(s) 299, 331, 563, 595, 2471, 2503
Asp700I GAANNNNTTC 2 cut(s) 89, 1710
Asp718I GGTACC 1 cut(s) 204
AspLEI GCGC 2 cut(s) 896, 2172
AspS9I GGNCC 6 cut(s) 496, 525, 915, 951, 1009, 2479
AsuC2I CCSGG 2 cut(s) 782, 1587
AsuHPI GGTGA 6 cut(s) 784, 1210, 1345, 1601, 2326, 2818
AsuNHI GCTAGC 2 cut(s) 857, 890
AvaI CYCGRG 1 cut(s) 3162
AvaII GGWCC 4 cut(s) 525, 915, 951, 2479
AxyI CCTNAGG 1 cut(s) 501
BalI TGGCCA 1 cut(s) 459
BamHI GGATCC 1 cut(s) 1912
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 3169
BbsI GAAGAC 4 cut(s) 82, 389, 1052, 2825
Bbv12I GWGCWC 3 cut(s) 409, 442, 2119
BceAI ACGGC 1 cut(s) 2079
BciT130I CCWGG 8 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2924
BclI TGATCA 2 cut(s) 2232, 2491
BcnI CCSGG 2 cut(s) 782, 1587
BcoDI GTCTC 3 cut(s) 251, 677, 3165
BcuI ACTAGT 1 cut(s) 3179
BfaI CTAG 5 cut(s) 648, 858, 891, 2120, 3180
BfmI CTRYAG 3 cut(s) 48, 1302, 1602
BfoI RGCGCY 1 cut(s) 2173
BfuAI ACCTGC 1 cut(s) 2573
BglII AGATCT 1 cut(s) 196
Bme18I GGWCC 4 cut(s) 525, 915, 951, 2479
BmeT110I CYCGRG 1 cut(s) 3162
BmgT120I GGNCC 6 cut(s) 496, 525, 915, 951, 1009, 2479
BmiI GGNNCC 8 cut(s) 206, 498, 756, 916, 917, 1098, 1914, 2480
BmrI ACTGGG 1 cut(s) 843
BmsI GCATC 5 cut(s) 197, 363, 532, 955, 3195
BmtI GCTAGC 2 cut(s) 861, 894
BmuI ACTGGG 1 cut(s) 843
BpiI GAAGAC 4 cut(s) 82, 389, 1052, 2825
BpmI CTGGAG 2 cut(s) 75, 3086
Bpu10I CCTNAGC 1 cut(s) 768
BpuEI CTTGAG 5 cut(s) 557, 701, 1169, 2504, 2756
BpuMI CCSGG 2 cut(s) 782, 1587
Bsa29I ATCGAT 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 251
BsaWI WCCGGW 1 cut(s) 2706
Bsc4I CCNNNNNNNGG 8 cut(s) 38, 39, 1081, 1664, 1727, 1850, 2087, 2218
Bse1I ACTGG 7 cut(s) 849, 975, 1141, 1773, 2010, 2807, 3069
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 3 cut(s) 868, 1321, 3088
BseAI TCCGGA 1 cut(s) 2706
BseBI CCWGG 8 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2924
BseCI ATCGAT 1 cut(s) 136
BseGI GGATG 9 cut(s) 547, 736, 1369, 2182, 2977, 3019, 3049, 3064, 3186
BseLI CCNNNNNNNGG 8 cut(s) 38, 39, 1081, 1664, 1727, 1850, 2087, 2218
BseMI GCAATG 3 cut(s) 868, 1321, 3088
BseMII CTCAG 6 cut(s) 492, 856, 1983, 2027, 2492, 3000
BseNI ACTGG 7 cut(s) 849, 975, 1141, 1773, 2010, 2807, 3069
BseRI GAGGAG 5 cut(s) 713, 2180, 2231, 2927, 3134
BseYI CCCAGC 1 cut(s) 1130
BsgI GTGCAG 3 cut(s) 319, 1566, 2240
Bsh1236I CGCG 1 cut(s) 896
BshFI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
BshNI GGYRCC 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 136
BsiHKAI GWGCWC 3 cut(s) 409, 442, 2119
BsiHKCI CYCGRG 1 cut(s) 3162
BsiSI CCGG 3 cut(s) 781, 1586, 2707
BslFI GGGAC 6 cut(s) 901, 1147, 1808, 1843, 2492, 2537
BslI CCNNNNNNNGG 8 cut(s) 38, 39, 1081, 1664, 1727, 1850, 2087, 2218
BsmAI GTCTC 3 cut(s) 251, 677, 3165
BsmFI GGGAC 6 cut(s) 901, 1147, 1808, 1843, 2492, 2537
BsnI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 3162
Bsp1286I GDGCHC 4 cut(s) 409, 442, 2119, 3169
Bsp13I TCCGGA 1 cut(s) 2706
Bsp19I CCATGG 1 cut(s) 2518
BspANI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
BspCNI CTCAG 6 cut(s) 493, 855, 1984, 2026, 2493, 3001
BspDI ATCGAT 1 cut(s) 136
BspEI TCCGGA 1 cut(s) 2706
BspFNI CGCG 1 cut(s) 896
BspHI TCATGA 4 cut(s) 273, 654, 1483, 1846
BspLI GGNNCC 8 cut(s) 206, 498, 756, 916, 917, 1098, 1914, 2480
BspMAI CTGCAG 2 cut(s) 52, 1606
BspMI ACCTGC 1 cut(s) 2573
BspOI GCTAGC 2 cut(s) 861, 894
BspPI GGATC 5 cut(s) 1585, 1732, 1907, 1920, 2713
BspQI GCTCTTC 1 cut(s) 2376
BspT107I GGYRCC 1 cut(s) 204
BspTNI GGTCTC 1 cut(s) 251
BsrBI CCGCTC 1 cut(s) 1192
BsrDI GCAATG 3 cut(s) 868, 1321, 3088
BsrI ACTGG 7 cut(s) 849, 975, 1141, 1773, 2010, 2807, 3069
BssT1I CCWWGG 7 cut(s) 345, 1870, 1908, 1916, 2334, 2518, 2647
Bst2UI CCWGG 8 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2924
Bst6I CTCTTC 4 cut(s) 1007, 1389, 2376, 3125
BstAPI GCANNNNNTGC 3 cut(s) 65, 1529, 3083
BstDSI CCRYGG 2 cut(s) 2095, 2518
BstF5I GGATG 9 cut(s) 547, 736, 1369, 2182, 2977, 3019, 3049, 3064, 3186
BstFNI CGCG 1 cut(s) 896
BstH2I RGCGCY 1 cut(s) 2173
BstHHI GCGC 2 cut(s) 896, 2172
BstMAI GTCTC 3 cut(s) 251, 677, 3165
BstNI CCWGG 8 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2924
BstNSI RCATGY 1 cut(s) 2204
BstSFI CTRYAG 3 cut(s) 48, 1302, 1602
BstUI CGCG 1 cut(s) 896
BstV2I GAAGAC 4 cut(s) 82, 389, 1052, 2825
BstX2I RGATCY 3 cut(s) 196, 1577, 1912
BstXI CCANNNNNNTGG 1 cut(s) 2033
BstYI RGATCY 3 cut(s) 196, 1577, 1912
Bsu15I ATCGAT 1 cut(s) 136
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
BsuTUI ATCGAT 1 cut(s) 136
BtgI CCRYGG 2 cut(s) 2095, 2518
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 9 cut(s) 547, 736, 1369, 2182, 2977, 3019, 3049, 3064, 3186
BtsIMutI CAGTG 6 cut(s) 856, 1060, 1299, 1859, 2003, 3121
BveI ACCTGC 1 cut(s) 2573
CaiI CAGNNNCTG 2 cut(s) 2815, 2900
CciI TCATGA 4 cut(s) 273, 654, 1483, 1846
CfoI GCGC 2 cut(s) 896, 2172
Cfr13I GGNCC 6 cut(s) 496, 525, 915, 951, 1009, 2479
ClaI ATCGAT 1 cut(s) 136
CseI GACGC 1 cut(s) 2874
Csp6I GTAC 4 cut(s) 205, 303, 1937, 2525
CviQI GTAC 4 cut(s) 205, 303, 1937, 2525
DraI TTTAAA 2 cut(s) 618, 1887
DraIII CACNNNGTG 1 cut(s) 1589
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
EaeI YGGCCR 2 cut(s) 457, 2092
Eam1104I CTCTTC 4 cut(s) 1007, 1389, 2376, 3125
EarI CTCTTC 4 cut(s) 1007, 1389, 2376, 3125
Eco130I CCWWGG 7 cut(s) 345, 1870, 1908, 1916, 2334, 2518, 2647
Eco147I AGGCCT 1 cut(s) 1448
Eco24I GRGCYC 1 cut(s) 3169
Eco31I GGTCTC 1 cut(s) 251
Eco32I GATATC 2 cut(s) 799, 2944
Eco47I GGWCC 4 cut(s) 525, 915, 951, 2479
Eco57I CTGAAG 5 cut(s) 76, 201, 1343, 1883, 2837
Eco81I CCTNAGG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 3162
EcoO109I RGGNCCY 4 cut(s) 496, 915, 951, 2479
EcoRII CCWGG 8 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2922
EcoRV GATATC 2 cut(s) 799, 2944
EcoT14I CCWWGG 7 cut(s) 345, 1870, 1908, 1916, 2334, 2518, 2647
EcoT22I ATGCAT 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 3169
ErhI CCWWGG 7 cut(s) 345, 1870, 1908, 1916, 2334, 2518, 2647
FalI AAGNNNNNCTT 4 cut(s) 1059, 1091, 2189, 2221
FaqI GGGAC 6 cut(s) 901, 1147, 1808, 1843, 2492, 2537
FauI CCCGC 5 cut(s) 40, 926, 1565, 2382, 2844
FauNDI CATATG 2 cut(s) 902, 991
FbaI TGATCA 2 cut(s) 2232, 2491
FokI GGATG 9 cut(s) 554, 743, 1376, 2189, 2984, 3026, 3056, 3071, 3173
FriOI GRGCYC 1 cut(s) 3169
FspBI CTAG 5 cut(s) 648, 858, 891, 2120, 3180
GlaI GCGC 2 cut(s) 895, 2171
GsaI CCCAGC 1 cut(s) 1134
GsuI CTGGAG 2 cut(s) 75, 3086
HaeII RGCGCY 1 cut(s) 2173
HaeIII GGCC 7 cut(s) 459, 497, 1010, 1146, 1448, 2094, 2646
HapII CCGG 3 cut(s) 781, 1586, 2707
HgaI GACGC 1 cut(s) 2874
HhaI GCGC 2 cut(s) 896, 2172
Hin6I GCGC 2 cut(s) 894, 2170
HinP1I GCGC 2 cut(s) 894, 2170
HincII GTYRAC 1 cut(s) 1778
HindII GTYRAC 1 cut(s) 1778
HindIII AAGCTT 3 cut(s) 533, 677, 3147
HpaII CCGG 3 cut(s) 781, 1586, 2707
HphI GGTGA 6 cut(s) 784, 1210, 1345, 1601, 2326, 2818
Hpy166II GTNNAC 2 cut(s) 1778, 2525
Hpy8I GTNNAC 2 cut(s) 1778, 2525
Hpy99I CGWCG 1 cut(s) 15
HpyCH4IV ACGT 2 cut(s) 2295, 2700
HpySE526I ACGT 2 cut(s) 2295, 2700
HspAI GCGC 2 cut(s) 894, 2170
KflI GGGWCCC 1 cut(s) 915
Kpn2I TCCGGA 1 cut(s) 2706
KpnI GGTACC 1 cut(s) 208
Ksp22I TGATCA 2 cut(s) 2232, 2491
LguI GCTCTTC 1 cut(s) 2376
LmnI GCTCC 6 cut(s) 56, 783, 1102, 1660, 1910, 2167
LweI GCATC 5 cut(s) 197, 363, 532, 955, 3195
MaeI CTAG 5 cut(s) 648, 858, 891, 2120, 3180
MaeII ACGT 2 cut(s) 2295, 2700
MbiI CCGCTC 1 cut(s) 1192
MflI RGATCY 3 cut(s) 196, 1577, 1912
MhlI GDGCHC 4 cut(s) 409, 442, 2119, 3169
MlsI TGGCCA 1 cut(s) 459
MluCI AATT 8 cut(s) 420, 547, 1103, 1114, 1626, 2300, 2659, 2875
MluNI TGGCCA 1 cut(s) 459
MlyI GAGTC 7 cut(s) 1825, 1988, 2708, 2906, 2924, 3014, 3041
MmeI TCCRAC 3 cut(s) 303, 457, 2889
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 1 cut(s) 378
MroI TCCGGA 1 cut(s) 2706
MroXI GAANNNNTTC 2 cut(s) 89, 1710
MscI TGGCCA 1 cut(s) 459
MslI CAYNNNNRTG 3 cut(s) 1926, 2133, 2270
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 5 cut(s) 53, 470, 635, 2219, 2389
MspI CCGG 3 cut(s) 781, 1586, 2707
MvaI CCWGG 8 cut(s) 949, 1041, 1827, 1975, 2112, 2158, 2174, 2924
MvnI CGCG 1 cut(s) 896
NciI CCSGG 2 cut(s) 782, 1587
NcoI CCATGG 1 cut(s) 2518
NdeI CATATG 2 cut(s) 902, 991
NheI GCTAGC 2 cut(s) 857, 890
NlaIV GGNNCC 8 cut(s) 206, 498, 756, 916, 917, 1098, 1914, 2480
NmuCI GTSAC 4 cut(s) 1224, 1333, 1589, 2466
NsiI ATGCAT 1 cut(s) 378
NspI RCATGY 1 cut(s) 2204
OliI CACNNNNGTG 1 cut(s) 1926
PaeI GCATGC 1 cut(s) 2204
PaeR7I CTCGAG 1 cut(s) 3162
PagI TCATGA 4 cut(s) 273, 654, 1483, 1846
PasI CCCWGGG 1 cut(s) 1826
PceI AGGCCT 1 cut(s) 1448
PciSI GCTCTTC 1 cut(s) 2376
PcsI WCGNNNNNNNCGW 1 cut(s) 2723
PdmI GAANNNNTTC 2 cut(s) 89, 1710
PleI GAGTC 7 cut(s) 1825, 1987, 2708, 2905, 2923, 3013, 3040
PpsI GAGTC 7 cut(s) 1825, 1987, 2708, 2905, 2923, 3013, 3040
PpuMI RGGWCCY 3 cut(s) 915, 951, 2479
Psp5II RGGWCCY 3 cut(s) 915, 951, 2479
Psp6I CCWGG 8 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2922
PspFI CCCAGC 1 cut(s) 1130
PspGI CCWGG 8 cut(s) 947, 1039, 1825, 1973, 2110, 2156, 2172, 2922
PspN4I GGNNCC 8 cut(s) 206, 498, 756, 916, 917, 1098, 1914, 2480
PspPI GGNCC 6 cut(s) 496, 525, 915, 951, 1009, 2479
PspPPI RGGWCCY 3 cut(s) 915, 951, 2479
PstI CTGCAG 2 cut(s) 52, 1606
PstNI CAGNNNCTG 2 cut(s) 2815, 2900
PsuI RGATCY 3 cut(s) 196, 1577, 1912
PvuII CAGCTG 2 cut(s) 53, 470
RsaI GTAC 4 cut(s) 206, 304, 1938, 2526
RsaNI GTAC 4 cut(s) 205, 303, 1937, 2525
RseI CAYNNNNRTG 3 cut(s) 1926, 2133, 2270
SapI GCTCTTC 1 cut(s) 2376
Sau96I GGNCC 6 cut(s) 496, 525, 915, 951, 1009, 2479
SchI GAGTC 7 cut(s) 1825, 1988, 2708, 2906, 2924, 3014, 3041
SduI GDGCHC 4 cut(s) 409, 442, 2119, 3169
SfaNI GCATC 5 cut(s) 197, 363, 532, 955, 3195
SfcI CTRYAG 3 cut(s) 48, 1302, 1602
Sfr274I CTCGAG 1 cut(s) 3162
SinI GGWCC 4 cut(s) 525, 915, 951, 2479
SlaI CTCGAG 1 cut(s) 3162
SmiMI CAYNNNNRTG 3 cut(s) 1926, 2133, 2270
SmlI CTYRAG 6 cut(s) 536, 680, 1148, 2483, 2771, 3162
SmoI CTYRAG 6 cut(s) 536, 680, 1148, 2483, 2771, 3162
SpeI ACTAGT 1 cut(s) 3179
SphI GCATGC 1 cut(s) 2204
Sse9I AATT 8 cut(s) 420, 547, 1103, 1114, 1626, 2300, 2659, 2875
SseBI AGGCCT 1 cut(s) 1448
SspI AATATT 4 cut(s) 229, 816, 943, 2194
SspMI CTAG 5 cut(s) 648, 858, 891, 2120, 3180
StuI AGGCCT 1 cut(s) 1448
StyI CCWWGG 7 cut(s) 345, 1870, 1908, 1916, 2334, 2518, 2647
TaiI ACGT 2 cut(s) 2298, 2703
TaqI TCGA 9 cut(s) 13, 136, 446, 2077, 2717, 2726, 2757, 2946, 3163
TaqII GACCGA 1 cut(s) 674
TasI AATT 8 cut(s) 420, 547, 1103, 1114, 1626, 2300, 2659, 2875
TauI GCSGC 1 cut(s) 635
TscAI CASTG 6 cut(s) 856, 1060, 1306, 1866, 2010, 3121
TseFI GTSAC 4 cut(s) 1224, 1333, 1589, 2466
Tsp45I GTSAC 4 cut(s) 1224, 1333, 1589, 2466
TspGWI ACGGA 4 cut(s) 416, 1145, 1634, 3036
TspRI CASTG 6 cut(s) 856, 1060, 1306, 1866, 2010, 3121
VpaK11BI GGWCC 4 cut(s) 525, 915, 951, 2479
XapI RAATTY 4 cut(s) 547, 1114, 2659, 2875
XbaI TCTAGA 1 cut(s) 2119
XceI RCATGY 1 cut(s) 2204
XcmI CCANNNNNNNNNTGG 2 cut(s) 35, 352
XhoI CTCGAG 1 cut(s) 3162
XmnI GAANNNNTTC 2 cut(s) 89, 1710
XspI CTAG 5 cut(s) 648, 858, 891, 2120, 3180
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.