pycom04g16700

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001181
Physical Location & Seq
Forward (+)
19338817 .. 19341762
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16700.1

Sequence Viewer

Length: 2946 bp
ATGGTTTTCATGAAGAAGCAGATTCATTTCTTGTGCAGTACAAAGAAGGTGTCTTTGTTGGAAGTTGTTAAAAAGTCTGCCAAGGAAGCTGTGGGTGTTGATGTTGTGATGCATGAGAAGACAAAGAGTGATGATGGAAGTGCTCTGATGGAGGCAATTTTCCGTGCCATTCGTGCTCAATCGAAGGCTGATGGCCATGACACAGCTGTCGTTGGACATATTGCAAGGGAGGCCCCTGAGGGAAACCTCTTGGAAACATGGTCCAAGAAGCTTGAGGATGCAAATTTTCAGTTGGGCGATGTAACAAATGGGTTGTCTGACCTGTTTGCTGTTAAGGACAATGACGAGATTTTAAATATCAAGAGAGCTGCTGTCTTGTCTGCTAGTGTCATGACCGAAGTAGTTGTTAAAAAGCTTGAGACTGTTATTGATGAGGAGAAGAAAGTCACCCATTCTTCTTTGATGGATGAGGCAGAGAAAGCTATTATGGAACCATCTAAAGCTAAAGTGAAGCTCCGGGCTGATAATGTTGATATCTGTTACCCCCCAATATTTCAGAGTGGAGGACAGTTTGATCTCAGACCCAGTGCTGCTAGCAATGATGAGTTGCTTTATTATGATTCTGCTAGCGCGATCATATGTGCTGTTGGGTCCCGCTACAAGAGTTACTGCTCAAATATTGCCAGGACCTTTCTGATTGATGCTACTTCCAGTCAGAGCAAGGCATATGAAGTTCTCTTCAAGGCCCATAATGCAGCCATTAGTGAGTTGAAGCCTGGGAAGAAGGTCAGTGCTGCTTATCAAGCTGCCCTTGCTGTGGTGGAGAAGGAGGCTCCTGAATTAGTATCAAATTTGACAAAATCGGCTGGGACTGGTATTGGCCTTGAGTTCCGTGAATCTGGGTTGAATATAAATGCAAAGAATGAGCGGTTGGTGAAAGTAGGCATGGTCTTTAATGTGTCACTTGGGTTTCAGAACTTACAGAGTGGTCGCAGCAATCCCAAAAACCAAAACTACGCTTTGTTGCTTGCGGACACTGTAGTTATTAGCAATGACAAGCCAGAGGTGGTGACAAGTAGAAGCTCTAAGAATCTTCAGGATGTGGCATACTCCTTTAATGATGGAGATGAAGAGGAAGCTGAAAAGCCCAAAAAACCGAAAGATGAGGCTAATGGAAGGGAGGCCTTAATGTCCAAGACAACATTGAGGTCAGACAATCATGAGATTTCGAAGGAGGAACTCCGCAGGCAACATCAGGCAGAACTTGCCCGTCAGAAAAATGAAGAAACTGCCCGCAGACTTGCTGGTGCTGGATCTGCACCGGGTGACAATCGTTCTGCAGCAAGGGCTTTAACGGATTTAATTGCCTACAAGAATGTAAATGATGTCGCTCCACCAAAAGGTCTGAATCAACTAATGATTCAGATTGACCAGAAGAATGAAGCTGTTCTGCTGCCTATTTATGGCAGTATGATCCCTTTTCATGTTGCAACCATAAGGACAGTTTCCAGTCAACAGGACACGAATCGGAACTGTTATATCAGAATCATGTTTAATGTCCCTGGGACTCCTTTTAGTCCTCATGATGCCAACTCACTGAAGAACCTTGGGTCTATATATTTAAAAGAAGTTTCATTTCGCTCCAAGGATCCAAGGCACATAAGTGAAGTGGTACAGGCGATTAAAGGGCTGCGAAGGCAAGTTGTGTCCAGGGAGTCCTTGATAGCTGAGAAGGCGACACTGGTTACTCAGGAGAAACTCCAAATAGCTGGTAACAGATTTAAGCCAATAAGATTGTCTGATCTTTGGATTCGACCTGTTTTTGGTGGCCGTGGAAGAAAGATTCCTGGTACTCTTGAAGCTCATGCAAATGGGTTTCGTTATTCTACCACCAGGCAAGAGGAGCGCCTGGATGTTATGTTTGCAAATATTAAGCATGCCTTTTTCCAGCCAGCGGAGAATGAGATGATCACTCTCCTCCACTTTTATCTGCACGATGACATCATGGTGGGCAACAAGAAAACAAAAAACGTGCAATTCTATGTTGAGGTGATGGACACAGTTCAGACCTTGGGAGGTAGTAGGAGGTCTGCCTATGACCCAGATGAGATTGAAGAAGAGCAGCGGGAGAGGGATAGGAAGAACAAAATAAATATGGATTTTCAGAACTTTGTGAATCGGGTTAATGAGCTTTGGGGGCAGTCACAGTTTAGGGACCTTGAGTTTGATCAACCTCTGAGAGAGCTTGGTTTCCATGGTGTACCTCATAAATCATCGGCATTTATTGTCCCTACTTCAACCTGCTTGGTTGAACTGATAGAAACTCCTTTCCTTGTTGTCAGTCTAAGCGAGATTGAGATTGTGAATCTGGAAAGAGTTGGCCTTGGGCAGAAGAATTTTGACATGACAATTGTATTCAAGGACTTCAAAAAGGACGTTCTCCGGATTGATTCGATCCCTTCAACAGCACTTGATGGTATCAAGGAATGGCTCGACACAACTGACCTCAAGTATTATGAGAGCAGGCTGAATCTCAACTGGAAACAGATACTGAAGACAATCACCGATGACCCGCAGAGCTTCATTGAAGATGGAGGTTGGGAATTTTTGAATTTGGAAGCGTCTGATTCAGAGTCTGAACAGTCGGAGGAGTCAGACCAGGGTTATGAACCATCAGATATCGAATCTGCTGAATCAGAAGAATCGGATGATGAGGATTCTGATAGTGAATCGGTTGTGGAGTCTGAGGATGAGGACGGAGAAGCGGAGTCAGATGAGGATGGGTTATCGTGGGATGAGCTGGAGAGGGAAGCAAGCAATGCAGATAGGGAGAAAGGGAACGAATCAGACAGTGAGGAGGACAGGAAGAGAAGGAAGGCGAAAGCTTTTGGGAAGTCTCGAGCCCCACCTCGTAGTAGCATCCCGAAGCGGAGTAAGATGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

982

Amino Acids

109.9

Weight (kDa)

5.33

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FACT-Spt16_Nlob PF14826 1 - 103 9.6e-21 FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24 PF00557 120 - 349 2e-29 Metallopeptidase family M24
SPT16 PF08644 474 - 587 9.1e-34 FACT complex subunit (SPT16/CDC68)
PH_SPT16 PF24824 597 - 728 2.7e-53 FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle PF08512 752 - 840 1.4e-23 Histone chaperone Rttp106-like, middle domain
SPT16_C PF21091 880 - 959 5.2e-14 FACT complex subunit SPT16, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 2309
AccBSI CCGCTC 1 cut(s) 928
AccII CGCG 1 cut(s) 632
AccIII TCCGGA 1 cut(s) 2442
AclWI GGATC 5 cut(s) 1321, 1468, 1643, 1656, 2449
AcoI YGGCCR 2 cut(s) 193, 1828
AcsI RAATTY 5 cut(s) 283, 850, 2395, 2603, 2611
AcuI CTGAAG 3 cut(s) 1079, 1619, 2573
AdeI CACNNNGTG 1 cut(s) 1325
AfaI GTAC 4 cut(s) 40, 1674, 1852, 2262
AfiI CCNNNNNNNGG 5 cut(s) 817, 1400, 1463, 1823, 1954
AjnI CCWGG 8 cut(s) 683, 775, 1561, 1709, 1846, 1892, 1908, 2658
AjuI GAANNNNNNNTTGG 2 cut(s) 914, 946
AleI CACNNNNGTG 1 cut(s) 1662
Alw21I GWGCWC 2 cut(s) 145, 178
Alw26I GTCTC 2 cut(s) 413, 2901
AlwI GGATC 5 cut(s) 1321, 1468, 1643, 1656, 2449
AlwNI CAGNNNCTG 2 cut(s) 2551, 2636
Ama87I CYCGRG 1 cut(s) 2898
Aor13HI TCCGGA 1 cut(s) 2442
AoxI GGCC 7 cut(s) 193, 231, 744, 880, 1182, 1828, 2380
ApoI RAATTY 5 cut(s) 283, 850, 2395, 2603, 2611
ArsI GACNNNNNNTTYG 6 cut(s) 35, 67, 299, 331, 2207, 2239
Asp700I GAANNNNTTC 1 cut(s) 1446
AspLEI GCGC 2 cut(s) 632, 1908
AspS9I GGNCC 6 cut(s) 232, 261, 651, 687, 745, 2215
AsuC2I CCSGG 2 cut(s) 518, 1323
AsuHPI GGTGA 6 cut(s) 439, 946, 1081, 1337, 2062, 2554
AsuII TTCGAA 1 cut(s) 1229
AsuNHI GCTAGC 2 cut(s) 593, 626
AvaI CYCGRG 1 cut(s) 2898
AvaII GGWCC 4 cut(s) 261, 651, 687, 2215
AxyI CCTNAGG 1 cut(s) 237
BalI TGGCCA 1 cut(s) 195
BamHI GGATCC 1 cut(s) 1648
BanII GRGCYC 1 cut(s) 2905
BbsI GAAGAC 2 cut(s) 125, 2561
Bbv12I GWGCWC 2 cut(s) 145, 178
BceAI ACGGC 1 cut(s) 1815
BciT130I CCWGG 8 cut(s) 685, 777, 1563, 1711, 1848, 1894, 1910, 2660
BclI TGATCA 2 cut(s) 1968, 2227
BcnI CCSGG 2 cut(s) 518, 1323
BcoDI GTCTC 2 cut(s) 413, 2901
BfaI CTAG 3 cut(s) 384, 594, 627
BfmI CTRYAG 2 cut(s) 1038, 1338
BfoI RGCGCY 1 cut(s) 1909
BfuAI ACCTGC 1 cut(s) 2309
Bme18I GGWCC 4 cut(s) 261, 651, 687, 2215
BmeT110I CYCGRG 1 cut(s) 2898
BmgT120I GGNCC 6 cut(s) 232, 261, 651, 687, 745, 2215
BmiI GGNNCC 7 cut(s) 234, 492, 652, 653, 834, 1650, 2216
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 5 cut(s) 99, 268, 691, 1576, 2928
BmtI GCTAGC 2 cut(s) 597, 630
BmuI ACTGGG 1 cut(s) 579
BpiI GAAGAC 2 cut(s) 125, 2561
BpmI CTGGAG 1 cut(s) 2822
Bpu14I TTCGAA 1 cut(s) 1229
BpuEI CTTGAG 5 cut(s) 293, 437, 905, 2240, 2492
BpuMI CCSGG 2 cut(s) 518, 1323
BsaWI WCCGGW 1 cut(s) 2442
Bsc4I CCNNNNNNNGG 5 cut(s) 817, 1400, 1463, 1823, 1954
Bse1I ACTGG 6 cut(s) 585, 711, 877, 1509, 1746, 2543
Bse21I CCTNAGG 1 cut(s) 237
Bse3DI GCAATG 3 cut(s) 604, 1057, 2824
BseAI TCCGGA 1 cut(s) 2442
BseBI CCWGG 8 cut(s) 685, 777, 1563, 1711, 1848, 1894, 1910, 2660
BseGI GGATG 9 cut(s) 283, 472, 1105, 1918, 2713, 2755, 2785, 2800, 2919
BseLI CCNNNNNNNGG 5 cut(s) 817, 1400, 1463, 1823, 1954
BseMI GCAATG 3 cut(s) 604, 1057, 2824
BseMII CTCAG 6 cut(s) 228, 592, 1719, 1763, 2228, 2736
BseNI ACTGG 6 cut(s) 585, 711, 877, 1509, 1746, 2543
BseRI GAGGAG 5 cut(s) 449, 1916, 1967, 2663, 2870
BseYI CCCAGC 1 cut(s) 866
BsgI GTGCAG 3 cut(s) 55, 1302, 1976
Bsh1236I CGCG 1 cut(s) 632
BshFI GGCC 7 cut(s) 195, 233, 746, 882, 1184, 1830, 2382
BsiHKAI GWGCWC 2 cut(s) 145, 178
BsiHKCI CYCGRG 1 cut(s) 2898
BsiSI CCGG 3 cut(s) 517, 1322, 2443
BslFI GGGAC 6 cut(s) 637, 883, 1544, 1579, 2228, 2273
BslI CCNNNNNNNGG 5 cut(s) 817, 1400, 1463, 1823, 1954
BsmAI GTCTC 2 cut(s) 413, 2901
BsmFI GGGAC 6 cut(s) 637, 883, 1544, 1579, 2228, 2273
BsnI GGCC 7 cut(s) 195, 233, 746, 882, 1184, 1830, 2382
BsoBI CYCGRG 1 cut(s) 2898
Bsp119I TTCGAA 1 cut(s) 1229
Bsp1286I GDGCHC 3 cut(s) 145, 178, 2905
Bsp13I TCCGGA 1 cut(s) 2442
Bsp143I GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
Bsp19I CCATGG 1 cut(s) 2254
BspANI GGCC 7 cut(s) 195, 233, 746, 882, 1184, 1830, 2382
BspCNI CTCAG 6 cut(s) 229, 591, 1720, 1762, 2229, 2737
BspEI TCCGGA 1 cut(s) 2442
BspFNI CGCG 1 cut(s) 632
BspHI TCATGA 4 cut(s) 9, 390, 1219, 1582
BspLI GGNNCC 7 cut(s) 234, 492, 652, 653, 834, 1650, 2216
BspMAI CTGCAG 1 cut(s) 1342
BspMI ACCTGC 1 cut(s) 2309
BspOI GCTAGC 2 cut(s) 597, 630
BspPI GGATC 5 cut(s) 1321, 1468, 1643, 1656, 2449
BspQI GCTCTTC 1 cut(s) 2112
BspT104I TTCGAA 1 cut(s) 1229
BsrBI CCGCTC 1 cut(s) 928
BsrDI GCAATG 3 cut(s) 604, 1057, 2824
BsrI ACTGG 6 cut(s) 585, 711, 877, 1509, 1746, 2543
BssMI GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
BssT1I CCWWGG 7 cut(s) 81, 1606, 1644, 1652, 2070, 2254, 2383
Bst2UI CCWGG 8 cut(s) 685, 777, 1563, 1711, 1848, 1894, 1910, 2660
Bst4CI ACNGT 9 cut(s) 424, 570, 1039, 1504, 1535, 2062, 2208, 2643, 2852
Bst6I CTCTTC 4 cut(s) 743, 1125, 2112, 2861
BstAPI GCANNNNNTGC 2 cut(s) 1265, 2819
BstBI TTCGAA 1 cut(s) 1229
BstC8I GCNNGC 9 cut(s) 595, 628, 1029, 1247, 1294, 1938, 1953, 2525, 2815
BstDEI CTNAG 8 cut(s) 237, 578, 1086, 1728, 1749, 2237, 2345, 2745
BstDSI CCRYGG 2 cut(s) 1831, 2254
BstF5I GGATG 9 cut(s) 283, 472, 1105, 1918, 2713, 2755, 2785, 2800, 2919
BstFNI CGCG 1 cut(s) 632
BstH2I RGCGCY 1 cut(s) 1909
BstHHI GCGC 2 cut(s) 632, 1908
BstKTI GATC 9 cut(s) 577, 636, 1316, 1476, 1651, 1804, 1971, 2230, 2457
BstMAI GTCTC 2 cut(s) 413, 2901
BstMBI GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
BstNI CCWGG 8 cut(s) 685, 777, 1563, 1711, 1848, 1894, 1910, 2660
BstNSI RCATGY 1 cut(s) 1940
BstSFI CTRYAG 2 cut(s) 1038, 1338
BstUI CGCG 1 cut(s) 632
BstV2I GAAGAC 2 cut(s) 125, 2561
BstX2I RGATCY 2 cut(s) 1313, 1648
BstXI CCANNNNNNTGG 1 cut(s) 1769
BstYI RGATCY 2 cut(s) 1313, 1648
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 7 cut(s) 195, 233, 746, 882, 1184, 1830, 2382
BtgI CCRYGG 2 cut(s) 1831, 2254
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 9 cut(s) 283, 472, 1105, 1918, 2713, 2755, 2785, 2800, 2919
BtsIMutI CAGTG 6 cut(s) 592, 796, 1035, 1595, 1739, 2857
BveI ACCTGC 1 cut(s) 2309
Cac8I GCNNGC 9 cut(s) 595, 628, 1029, 1247, 1294, 1938, 1953, 2525, 2815
CaiI CAGNNNCTG 2 cut(s) 2551, 2636
CciI TCATGA 4 cut(s) 9, 390, 1219, 1582
CfoI GCGC 2 cut(s) 632, 1908
Cfr13I GGNCC 6 cut(s) 232, 261, 651, 687, 745, 2215
CseI GACGC 1 cut(s) 2610
Csp6I GTAC 4 cut(s) 39, 1673, 1851, 2261
CviQI GTAC 4 cut(s) 39, 1673, 1851, 2261
DdeI CTNAG 8 cut(s) 237, 578, 1086, 1728, 1749, 2237, 2345, 2745
DpnI GATC 9 cut(s) 576, 635, 1315, 1475, 1650, 1803, 1970, 2229, 2456
DpnII GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
DraI TTTAAA 2 cut(s) 354, 1623
DraIII CACNNNGTG 1 cut(s) 1325
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 2 cut(s) 193, 1828
Eam1104I CTCTTC 4 cut(s) 743, 1125, 2112, 2861
EarI CTCTTC 4 cut(s) 743, 1125, 2112, 2861
Eco130I CCWWGG 7 cut(s) 81, 1606, 1644, 1652, 2070, 2254, 2383
Eco147I AGGCCT 1 cut(s) 1184
Eco24I GRGCYC 1 cut(s) 2905
Eco32I GATATC 2 cut(s) 535, 2680
Eco47I GGWCC 4 cut(s) 261, 651, 687, 2215
Eco57I CTGAAG 3 cut(s) 1079, 1619, 2573
Eco81I CCTNAGG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 2898
EcoO109I RGGNCCY 4 cut(s) 232, 651, 687, 2215
EcoRII CCWGG 8 cut(s) 683, 775, 1561, 1709, 1846, 1892, 1908, 2658
EcoRV GATATC 2 cut(s) 535, 2680
EcoT14I CCWWGG 7 cut(s) 81, 1606, 1644, 1652, 2070, 2254, 2383
EcoT22I ATGCAT 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 2905
ErhI CCWWGG 7 cut(s) 81, 1606, 1644, 1652, 2070, 2254, 2383
FalI AAGNNNNNCTT 4 cut(s) 795, 827, 1925, 1957
FaqI GGGAC 6 cut(s) 637, 883, 1544, 1579, 2228, 2273
FauI CCCGC 4 cut(s) 662, 1301, 2118, 2580
FauNDI CATATG 2 cut(s) 638, 727
FbaI TGATCA 2 cut(s) 1968, 2227
FokI GGATG 9 cut(s) 290, 479, 1112, 1925, 2720, 2762, 2792, 2807, 2906
FriOI GRGCYC 1 cut(s) 2905
FspBI CTAG 3 cut(s) 384, 594, 627
GlaI GCGC 2 cut(s) 631, 1907
GsaI CCCAGC 1 cut(s) 870
GsuI CTGGAG 1 cut(s) 2822
HaeII RGCGCY 1 cut(s) 1909
HaeIII GGCC 7 cut(s) 195, 233, 746, 882, 1184, 1830, 2382
HapII CCGG 3 cut(s) 517, 1322, 2443
HgaI GACGC 1 cut(s) 2610
HhaI GCGC 2 cut(s) 632, 1908
Hin6I GCGC 2 cut(s) 630, 1906
HinP1I GCGC 2 cut(s) 630, 1906
HincII GTYRAC 1 cut(s) 1514
HindII GTYRAC 1 cut(s) 1514
HindIII AAGCTT 3 cut(s) 269, 413, 2883
HpaII CCGG 3 cut(s) 517, 1322, 2443
HphI GGTGA 6 cut(s) 439, 946, 1081, 1337, 2062, 2554
Hpy166II GTNNAC 2 cut(s) 1514, 2261
Hpy8I GTNNAC 2 cut(s) 1514, 2261
HpyCH4III ACNGT 9 cut(s) 424, 570, 1039, 1504, 1535, 2062, 2208, 2643, 2852
HpyCH4IV ACGT 2 cut(s) 2031, 2436
HpyF3I CTNAG 8 cut(s) 237, 578, 1086, 1728, 1749, 2237, 2345, 2745
HpySE526I ACGT 2 cut(s) 2031, 2436
HspAI GCGC 2 cut(s) 630, 1906
KflI GGGWCCC 1 cut(s) 651
Kpn2I TCCGGA 1 cut(s) 2442
Ksp22I TGATCA 2 cut(s) 1968, 2227
Kzo9I GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
LguI GCTCTTC 1 cut(s) 2112
LmnI GCTCC 5 cut(s) 519, 838, 1396, 1646, 1903
LweI GCATC 5 cut(s) 99, 268, 691, 1576, 2928
MaeI CTAG 3 cut(s) 384, 594, 627
MaeII ACGT 2 cut(s) 2031, 2436
MalI GATC 9 cut(s) 576, 635, 1315, 1475, 1650, 1803, 1970, 2229, 2456
MbiI CCGCTC 1 cut(s) 928
MboI GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
MfeI CAATTG 1 cut(s) 2409
MflI RGATCY 2 cut(s) 1313, 1648
MhlI GDGCHC 3 cut(s) 145, 178, 2905
MlsI TGGCCA 1 cut(s) 195
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 6 cut(s) 1561, 1724, 2642, 2660, 2750, 2777
MmeI TCCRAC 3 cut(s) 39, 193, 2625
Mox20I TGGCCA 1 cut(s) 195
Mph1103I ATGCAT 1 cut(s) 114
MroI TCCGGA 1 cut(s) 2442
MroXI GAANNNNTTC 1 cut(s) 1446
MscI TGGCCA 1 cut(s) 195
MslI CAYNNNNRTG 3 cut(s) 1662, 1869, 2006
Msp20I TGGCCA 1 cut(s) 195
MspA1I CMGCKG 3 cut(s) 206, 1955, 2125
MspI CCGG 3 cut(s) 517, 1322, 2443
MunI CAATTG 1 cut(s) 2409
MvaI CCWGG 8 cut(s) 685, 777, 1563, 1711, 1848, 1894, 1910, 2660
MvnI CGCG 1 cut(s) 632
NciI CCSGG 2 cut(s) 518, 1323
NcoI CCATGG 1 cut(s) 2254
NdeI CATATG 2 cut(s) 638, 727
NdeII GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
NheI GCTAGC 2 cut(s) 593, 626
NlaIV GGNNCC 7 cut(s) 234, 492, 652, 653, 834, 1650, 2216
NmuCI GTSAC 5 cut(s) 445, 960, 1069, 1325, 2202
NsiI ATGCAT 1 cut(s) 114
NspI RCATGY 1 cut(s) 1940
NspV TTCGAA 1 cut(s) 1229
OliI CACNNNNGTG 1 cut(s) 1662
PaeI GCATGC 1 cut(s) 1940
PaeR7I CTCGAG 1 cut(s) 2898
PagI TCATGA 4 cut(s) 9, 390, 1219, 1582
PasI CCCWGGG 1 cut(s) 1562
PceI AGGCCT 1 cut(s) 1184
PciSI GCTCTTC 1 cut(s) 2112
PdmI GAANNNNTTC 1 cut(s) 1446
PleI GAGTC 6 cut(s) 1561, 1723, 2641, 2659, 2749, 2776
PpsI GAGTC 6 cut(s) 1561, 1723, 2641, 2659, 2749, 2776
PpuMI RGGWCCY 3 cut(s) 651, 687, 2215
Psp5II RGGWCCY 3 cut(s) 651, 687, 2215
Psp6I CCWGG 8 cut(s) 683, 775, 1561, 1709, 1846, 1892, 1908, 2658
PspFI CCCAGC 1 cut(s) 866
PspGI CCWGG 8 cut(s) 683, 775, 1561, 1709, 1846, 1892, 1908, 2658
PspN4I GGNNCC 7 cut(s) 234, 492, 652, 653, 834, 1650, 2216
PspPI GGNCC 6 cut(s) 232, 261, 651, 687, 745, 2215
PspPPI RGGWCCY 3 cut(s) 651, 687, 2215
PstI CTGCAG 1 cut(s) 1342
PstNI CAGNNNCTG 2 cut(s) 2551, 2636
PsuI RGATCY 2 cut(s) 1313, 1648
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 4 cut(s) 40, 1674, 1852, 2262
RsaNI GTAC 4 cut(s) 39, 1673, 1851, 2261
RseI CAYNNNNRTG 3 cut(s) 1662, 1869, 2006
SapI GCTCTTC 1 cut(s) 2112
Sau3AI GATC 9 cut(s) 574, 633, 1313, 1473, 1648, 1801, 1968, 2227, 2454
Sau96I GGNCC 6 cut(s) 232, 261, 651, 687, 745, 2215
SchI GAGTC 6 cut(s) 1561, 1724, 2642, 2660, 2750, 2777
SduI GDGCHC 3 cut(s) 145, 178, 2905
SfaNI GCATC 5 cut(s) 99, 268, 691, 1576, 2928
SfcI CTRYAG 2 cut(s) 1038, 1338
Sfr274I CTCGAG 1 cut(s) 2898
SfuI TTCGAA 1 cut(s) 1229
SinI GGWCC 4 cut(s) 261, 651, 687, 2215
SlaI CTCGAG 1 cut(s) 2898
SmiMI CAYNNNNRTG 3 cut(s) 1662, 1869, 2006
SmlI CTYRAG 6 cut(s) 272, 416, 884, 2219, 2507, 2898
SmoI CTYRAG 6 cut(s) 272, 416, 884, 2219, 2507, 2898
SphI GCATGC 1 cut(s) 1940
SseBI AGGCCT 1 cut(s) 1184
SspI AATATT 3 cut(s) 552, 679, 1930
SspMI CTAG 3 cut(s) 384, 594, 627
StuI AGGCCT 1 cut(s) 1184
StyI CCWWGG 7 cut(s) 81, 1606, 1644, 1652, 2070, 2254, 2383
TaaI ACNGT 9 cut(s) 424, 570, 1039, 1504, 1535, 2062, 2208, 2643, 2852
TaiI ACGT 2 cut(s) 2034, 2439
TaqI TCGA 7 cut(s) 182, 1229, 1813, 2453, 2493, 2682, 2899
TaqII GACCGA 1 cut(s) 410
TatI WGTACW 1 cut(s) 38
TscAI CASTG 6 cut(s) 592, 796, 1042, 1602, 1746, 2857
TseFI GTSAC 5 cut(s) 445, 960, 1069, 1325, 2202
Tsp45I GTSAC 5 cut(s) 445, 960, 1069, 1325, 2202
TspGWI ACGGA 4 cut(s) 152, 881, 1370, 2772
TspRI CASTG 6 cut(s) 592, 796, 1042, 1602, 1746, 2857
VpaK11BI GGWCC 4 cut(s) 261, 651, 687, 2215
XapI RAATTY 5 cut(s) 283, 850, 2395, 2603, 2611
XceI RCATGY 1 cut(s) 1940
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XhoI CTCGAG 1 cut(s) 2898
XmnI GAANNNNTTC 1 cut(s) 1446
XspI CTAG 3 cut(s) 384, 594, 627
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.