Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001181

Physical Location & Seq

Genomic Coordinates Reverse (-)

28212728 .. 28217657

Gene Structure 4930 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1201600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3219 bp

ATGGCTGAGCAACGTAAAGGTAATGTCAAACCTGCAAATAGGAAGCCATCAGCAACCGCCACAGCAACCACCAGTGCATATTCCATTGATCTCAATAACTTCAGCAAGCGGTTAAAGATGATGTATTCGCATTGGAGGGAACATCGTAGTGATGTATGGGGTGAATCTGATGCACTTGCAATAGCAACTCCTCCAAATTCTGAGGATCTGCGGTATCTGAAATCCTCAGCAATGAATATCTGGTTGCTCGGTTATGAGTTCCCAGAAACTATCATGGTTTTCTCAAAGAAGCAGATCCATGTTTTGTGTAGCCAGAAAAAGGCTTCTCTCCTTGATGTTGTGATAAAGCCTGCAAAAGAGGCTGTTGGTGTTGAAGTTGTGATGCATGTGAAACTCAAAGGCCAGGATGGAACTGGACTGATGGATAGCATATTTCGAGCTATAAATGCCCACTTGAGCTCCGATGCTCCTGTGGTTGGACACATAGCAAGAGAGGCTCCTGAAGGGAAACTTTTGGAGACTTGGAGTGAGAAGTTGAAGGATGCTAATTTTGAGTTGAGTGATGTGACCAATGGGTTCTCAGACTTGTTTGCCGTCAAAGACCTGGTTGAGCTTACATATGTGAAGAAAGCCGCGTACTTGACTTCATCAGTGATGAAGACATTTGTGGTACCTAAGCTTGAGAAGGTCATTGATGAGGAAAAGAAAATTTCCCATTCTTCATTGATGGATGACACGGAAAAGGTCATATTGGAGCCTGCAAGAATTAAGGTGAAGCTGACAGCAGCAAATGTTGATATTTGCTATCCTCCAATTTTTCAGAGTGGAGGAGAATTTGATCTGAAGCCAAGTGCTTCAAGCAATGATGAGAACCTTTGTTATGACTCTACTAGTGTGATTATATGTGCTGTTGGATCAAGGTACAAAAGCTACTGCTCAAATATTGCTCGAACCTATCTGATTGATGCAAATTCTACGCAGAGCAAGGCATATGAGGTTCTGCTTGAAGCTCAAGAACCTGCAATCGGTAATTTAAAATCTGGGAAGAAGTTAAGTGCTGCATACCAAGCTGCACTCTCAGTAGTTGAAAGGGAGGCTCCAGAGCTGACTGGAAACTTGACCAAAACAGCTGGAACTGGAATTGGCCTTGAATTTCGTGAGTCAGGGCTTAATCTTAATGCTAAGAATGATCGAGTTTTGAGATCAGGCATGGTTTTCAACGTTTCTCTGGGTTTCCAGAATGTGCAGGCACAGACAAAAGACCCTAAGACCCAGATATTTTCACTGTTAATAGCAGATACAGTTATTGTTGGTAAAGAGACCCCAGAAGTATTGACTAATTCAAGCAAAGCTGTTAAGGATGTGGCGTACTCATTCAATGATGACGATGATGAAGAAGAAGAGCGTGCAAAACCTATATCCAGTAGTAAAGGTGCCGGGAGTGCCATGAGTAAGGCCACGCTTAGATCAGACAACCATGAAATGTCTAAAGAAGAGCTACGAAGGCAGCACCAAGCCGAACTTGCCCGCCAAAAGAATGAAGAAACTGCTAGAAGGCTTGCTGGTGGGGGTTCTACAGCTAACGATAATCGTGGTGCTGGGAAGACGGTTGGTGATCTGATTGCATATAAGAATGTCAATGATCTGCCTGCTCCAAAAGAGTTGATGATTCAGGTTGACCAGAGGAATGAAGCTATCCTCTTGCCGGTTTATGGAAACATGATTCCTTTTCACATAGCCACTGTGAAGAGTGTTTCCAGCCAGCAGGACACTAACCGGAATTGCTACATCCGTATAATCTTTAATGTACCAGGTACCCCATTTAGCCCTCATGATGCAAACACCCTGAAGTTTCAAGGGTCAACTTATTTAAAGGAAGTTTCATTCCGTTCCAAGGACCCAAGGCATATAAGTGAAGTAGTACAGCAAATTAAAACTCTTCGCAGACAGGTTGCCTCAAGGGAGTCTGAAAGAGCTGAAAGGGCAACTTTAGTCACACAGGAGAAGCTGCAAATTTCAGGAGCCAAATTCAAGCCAAAAAGATTGCCAGATCTATGGATTCGTCCTGTTTTTGGGGGTCGTGGAAGAAAGCTCACTGGATCACTGGAAGCCCACACAAATGGGTTCCGGTATTCTACTTCAAGGTCTGAAGAACGTGTCGATGTTATGTTTAGCAATATCAAACATGCATTTTTCCAGCCAGCTGAGAAGGAAATGATTACCCTGCTGCACTTTCATCTGCACGATCATATTATGGTAGGAAACAAAAAGACCAAGGACGTGCAATTTTATGCGGAGGTGATGGATGTAGTGCAGACACTGGGAGGTGGAAGGAGGTCAAACTATGATCCTGATGAGATCGAGGAAGAGCAACGTGAAAGGGAACGAAAGAACAAAATTAATATGGAGTTCCAGAACTTTGTGAACCGAGTAAATGATTCGTGGGGGCAACCTCCATTCAAATCACTTGATCTTGAGTTTGATCAGCCCCTTAGGGAGCTTGGCTTCCATGGAGTACCTCATAAAGCCTCGGCTTACATTGTCCCAACTTCAAGCTGTCTGGTTGAGCTGATAGAGACACCGTTTGTGGTAATTACCCTGAGCGAGATTGAAATAGTAAACCTTGAGAGAGTTGGTTTAGGGCAAAAGAACTTTGATTTGACTGTTGTTTTCAAGGACTTCAAGCGAGATGTCTTCCGAATTGATTCCATTCCCTCGACATCACTGGATGGCATTAAGGAGTGGTTAGACACAACTGATCTCAAGTATTATGAGAGTAGGTTGAATCTCAACTGGCGTCCTATTCTCAAAACCATTACTGACGACCCTGAGAAGTTCATAGAGGATGGTGGATGGGAATTTTTGAACATGGATATTAGTGATTCGGATTCCGACAATTCTCAGGAGTCTGACCGTGGGTATGTGCCTTCAGATGTGCAGTCGGATTCAGGTTCAGATGAGGAGGATCATGACAGCGAGTCATTAGTGGAGTCGGAAGATGATGAGGAAGAAGAGTCCGGAGAAGACTCAGAAGAGGCCGAAGGAAAGACATGGGAAGAGTTGGAGAGGGAAGCAAGCCACGCAGACAGGGAAAAAGGGAATGCGTCTGACAGTGAAGAGGAGAGGGCCAGAAGGAAGGTTAAGGCTTTTGGAAAGGCTCGAGTACCCGACAAGAGGAACCTTGGTGGCAGCCTTCCCAAGAGGCCAAAATTTAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

1073

Amino Acids

120.22

Weight (kDa)

5.63

Isoelectric Point (pI)

44.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FACT-Spt16_Nlob	PF14826	29 - 192	1.7e-48	FACT complex subunit SPT16 N-terminal lobe domain
Peptidase_M24	PF00557	208 - 437	1.2e-26	Metallopeptidase family M24
SPT16	PF08644	557 - 670	4.3e-37	FACT complex subunit (SPT16/CDC68)
PH_SPT16	PF24824	681 - 811	1.3e-55	FACT complex subunit SPT16 PH-like domain
Rttp106-like_middle	PF08512	838 - 925	3.3e-23	Histone chaperone Rttp106-like, middle domain
SPT16_C	PF21091	964 - 1046	7.3e-16	FACT complex subunit SPT16, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10710 AT4G10710 AT4G10710
fragaria_vesca	FvH4_1g19730 FvH4_1g19730 FvH4_1g19730 FvH4_6g08280
malus_domestica	MD04G1188700.v1.1 MD04G1188800.v1.1 MD12G1201500.v1.1 MD12G1201600.v1.1
prunus_persica	Prupe.6G306600_v2.0.a1 Prupe.6G306700_v2.0.a1 Prupe.6G306700_v2.0.a1
pyrus_communis	pycom04g16700 pycom12g18760 pycom12g18770 pycom12g18790
rosa_chinensis	RchiOBHm_Chr2g0111311 RchiOBHm_Chr3g0458161 RchiOBHm_Chr3g0460191
rosa_laevigata	RLG00000017884 RLG00000025036 RLG00000025174
rosa_multiflora	Rmu_co8443511.1_g000001 Rmu_sc0000449.1_g000044 Rmu_sc0001363.1_g000004 Rmu_sc0003763.1_g000005 Rmu_sc0005033.1_g000004 Rmu_sc0019318.1_g000002
rosa_roxburghii	Rroxscaffold_2G00132510 Rroxscaffold_6G00419750 Rroxscaffold_6G00421250
rosa_rugosa	Rorug02G0172800 Rorug02G0172800 Rorug03G0025200 Rorug03G0040700 Rorug03G0040800 Rorug03G0040900 Rorug03G0041000 Rorug03G0041000 Rorug03G0041100
rosa_samantha	Rh2AG225800 Rh2BG237500 Rh2CG228800 Rh2DG233300 Rh3AG086000 Rh3AG098500 Rh3BG088700 Rh3BG101700 Rh3CG089300 Rh3CG103800 Rh3DG089700 Rh3DG103000
rosa_wichuraiana	Rw2G017430 Rw3G007300 Rw3G008330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	40, 1027
Acc65I	GGTACC	2 cut(s)	670, 1814
AccB1I	GGYRCC	3 cut(s)	670, 1433, 1814
AccII	CGCG	1 cut(s)	635
AccIII	TCCGGA	1 cut(s)	3017
AciI	CCGC	6 cut(s)	57, 109, 211, 633, 1528, 2294
AclI	AACGTT	1 cut(s)	1221
AclWI	GGATC	6 cut(s)	213, 289, 922, 2107, 2342, 2973
AcsI	RAATTY	9 cut(s)	196, 708, 833, 970, 1151, 2013, 2027, 2858, 3209
AcuI	CTGAAG	6 cut(s)	85, 522, 863, 1868, 2169, 2913
AcyI	GRCGYC	1 cut(s)	2797
AfaI	GTAC	9 cut(s)	638, 672, 923, 1370, 1809, 1816, 1923, 2517, 3165
AfiI	CCNNNNNNNGG	8 cut(s)	319, 476, 1025, 1705, 1712, 1894, 2072, 3174
AflIII	ACRYGT	1 cut(s)	2155
AhdI	GACNNNNNGTC	1 cut(s)	2977
AhlI	ACTAGT	1 cut(s)	890
AjiI	CACGTC	1 cut(s)	2281
AjnI	CCWGG	3 cut(s)	402, 603, 1810
Alw21I	GWGCWC	1 cut(s)	461
Alw26I	GTCTC	3 cut(s)	512, 1313, 2570
AlwI	GGATC	6 cut(s)	213, 289, 922, 2107, 2342, 2973
AlwNI	CAGNNNCTG	1 cut(s)	2320
Ama87I	CYCGRG	1 cut(s)	3159
Aor13HI	TCCGGA	1 cut(s)	3017
AoxI	GGCC	6 cut(s)	400, 1144, 1455, 3036, 3126, 3203
ApeKI	GCWGC	7 cut(s)	785, 1058, 1070, 1507, 2008, 2227, 3189
ApoI	RAATTY	9 cut(s)	196, 708, 833, 970, 1151, 2013, 2027, 2858, 3209
AseI	ATTAAT	1 cut(s)	2400
Asp700I	GAANNNNTTC	1 cut(s)	2704
Asp718I	GGTACC	2 cut(s)	670, 1814
AspS9I	GGNCC	2 cut(s)	1897, 3126
AsuC2I	CCSGG	1 cut(s)	1438
AsuHPI	GGTGA	4 cut(s)	173, 784, 1625, 2311
AvaI	CYCGRG	1 cut(s)	3159
AvaII	GGWCC	1 cut(s)	1897
AxyI	CCTNAGG	1 cut(s)	2492
BanI	GGYRCC	3 cut(s)	670, 1433, 1814
BanII	GRGCYC	1 cut(s)	461
BbsI	GAAGAC	4 cut(s)	665, 1610, 2686, 3030
Bbv12I	GWGCWC	1 cut(s)	461
BbvCI	CCTCAGC	1 cut(s)	226
BbvI	GCAGC	7 cut(s)	797, 1045, 1057, 1519, 1995, 2214, 3201
BccI	CCATC	8 cut(s)	55, 401, 415, 721, 2296, 2723, 2840, 2847
BceAI	ACGGC	1 cut(s)	578
BciT130I	CCWGG	3 cut(s)	404, 605, 1812
BclI	TGATCA	1 cut(s)	2482
BcnI	CCSGG	1 cut(s)	1438
BcoDI	GTCTC	3 cut(s)	512, 1313, 2570
BcuI	ACTAGT	1 cut(s)	890
BfaI	CTAG	2 cut(s)	891, 1551
BfmI	CTRYAG	1 cut(s)	1575
BfuAI	ACCTGC	2 cut(s)	40, 1027
BglII	AGATCT	1 cut(s)	2050
BisI	GCNGC	8 cut(s)	633, 786, 1059, 1071, 1508, 2009, 2228, 3190
BlpI	GCTNAGC	1 cut(s)	6
BlsI	GCNGC	8 cut(s)	634, 787, 1060, 1072, 1509, 2010, 2229, 3191
Bme1390I	CCNGG	4 cut(s)	404, 605, 1438, 1812
Bme18I	GGWCC	1 cut(s)	1897
BmeRI	GACNNNNNGTC	1 cut(s)	2977
BmeT110I	CYCGRG	1 cut(s)	3159
BmgBI	CACGTC	1 cut(s)	2281
BmgT120I	GGNCC	2 cut(s)	1897, 3126
BmrFI	CCNGG	4 cut(s)	404, 605, 1438, 1812
BmrI	ACTGGG	1 cut(s)	2330
BmsI	GCATC	6 cut(s)	160, 372, 454, 532, 955, 1825
BmuI	ACTGGG	1 cut(s)	2330
BpiI	GAAGAC	4 cut(s)	665, 1610, 2686, 3030
BpmI	CTGGAG	1 cut(s)	1083
Bpu10I	CCTNAGC	3 cut(s)	226, 675, 2600
Bpu1102I	GCTNAGC	1 cut(s)	6
BpuEI	CTTGAG	7 cut(s)	475, 701, 996, 1942, 2495, 2645, 2747
BpuMI	CCSGG	1 cut(s)	1438
BsaHI	GRCGYC	1 cut(s)	2797
BsaI	GGTCTC	1 cut(s)	1313
BsaJI	CCNNGG	7 cut(s)	1893, 1901, 2274, 2509, 2529, 2914, 3181
BsaWI	WCCGGW	3 cut(s)	1776, 2127, 3017
BsaXI	ACNNNNNCTCC	2 cut(s)	443, 473
Bsc4I	CCNNNNNNNGG	8 cut(s)	319, 476, 1025, 1705, 1712, 1894, 2072, 3174
Bse118I	RCCGGY	1 cut(s)	1705
Bse21I	CCTNAGG	1 cut(s)	2492
Bse3DI	GCAATG	2 cut(s)	237, 868
BseAI	TCCGGA	1 cut(s)	3017
BseBI	CCWGG	3 cut(s)	404, 605, 1812
BseDI	CCNNGG	7 cut(s)	1893, 1901, 2274, 2509, 2529, 2914, 3181
BseGI	GGATG	9 cut(s)	412, 547, 736, 1366, 1788, 2311, 2734, 2851, 2858
BseLI	CCNNNNNNNGG	8 cut(s)	319, 476, 1025, 1705, 1712, 1894, 2072, 3174
BseMI	GCAATG	2 cut(s)	237, 868
BseMII	CTCAG	9 cut(s)	192, 240, 594, 1092, 2196, 2591, 2820, 2915, 3042
BseRI	GAGGAG	4 cut(s)	180, 843, 2975, 3134
BseXI	GCAGC	7 cut(s)	797, 1045, 1057, 1519, 1995, 2214, 3201
BseYI	CCCAGC	1 cut(s)	1598
BsgI	GTGCAG	6 cut(s)	1056, 1265, 2213, 2225, 2333, 2957
Bsh1236I	CGCG	1 cut(s)	635
BshFI	GGCC	6 cut(s)	402, 1146, 1457, 3038, 3128, 3205
BshNI	GGYRCC	3 cut(s)	670, 1433, 1814
BsiHKAI	GWGCWC	1 cut(s)	461
BsiHKCI	CYCGRG	1 cut(s)	3159
BsiSI	CCGG	5 cut(s)	1437, 1706, 1777, 2128, 3018
BslFI	GGGAC	1 cut(s)	2528
BslI	CCNNNNNNNGG	8 cut(s)	319, 476, 1025, 1705, 1712, 1894, 2072, 3174
BsmAI	GTCTC	3 cut(s)	512, 1313, 2570
BsmFI	GGGAC	1 cut(s)	2528
BsmI	GAATGC	1 cut(s)	3106
BsnI	GGCC	6 cut(s)	402, 1146, 1457, 3038, 3128, 3205
Bso31I	GGTCTC	1 cut(s)	1313
BsoBI	CYCGRG	1 cut(s)	3159
Bsp1286I	GDGCHC	1 cut(s)	461
Bsp13I	TCCGGA	1 cut(s)	3017
Bsp1720I	GCTNAGC	1 cut(s)	6
Bsp19I	CCATGG	1 cut(s)	2509
BspACI	CCGC	6 cut(s)	57, 109, 211, 633, 1528, 2294
BspANI	GGCC	6 cut(s)	402, 1146, 1457, 3038, 3128, 3205
BspCNI	CTCAG	9 cut(s)	193, 239, 593, 1091, 2197, 2592, 2821, 2914, 3041
BspEI	TCCGGA	1 cut(s)	3017
BspFNI	CGCG	1 cut(s)	635
BspHI	TCATGA	2 cut(s)	1831, 2968
BspMI	ACCTGC	2 cut(s)	40, 1027
BspPI	GGATC	6 cut(s)	213, 289, 922, 2107, 2342, 2973
BspQI	GCTCTTC	3 cut(s)	1395, 1488, 2361
BspT107I	GGYRCC	3 cut(s)	670, 1433, 1814
BspTNI	GGTCTC	1 cut(s)	1313
BsrDI	GCAATG	2 cut(s)	237, 868
BsrFI	RCCGGY	1 cut(s)	1705
BssAI	RCCGGY	1 cut(s)	1705
BssECI	CCNNGG	7 cut(s)	1893, 1901, 2274, 2509, 2529, 2914, 3181
BssNI	GRCGYC	1 cut(s)	2797
BssT1I	CCWWGG	5 cut(s)	1893, 1901, 2274, 2509, 3181
Bst2UI	CCWGG	3 cut(s)	404, 605, 1812
Bst4CI	ACNGT	8 cut(s)	1287, 1303, 1609, 1744, 2583, 2665, 2915, 3113
Bst6I	CTCTTC	9 cut(s)	1395, 1488, 1742, 1944, 2361, 3006, 3027, 3051, 3111
BstACI	GRCGYC	1 cut(s)	2797
BstDSI	CCRYGG	2 cut(s)	2509, 2914
BstF5I	GGATG	9 cut(s)	412, 547, 736, 1366, 1788, 2311, 2734, 2851, 2858
BstFNI	CGCG	1 cut(s)	635
BstMAI	GTCTC	3 cut(s)	512, 1313, 2570
BstMWI	GCNNNNNNNGC	9 cut(s)	359, 446, 494, 1067, 1442, 1504, 1523, 1982, 3080
BstNI	CCWGG	3 cut(s)	404, 605, 1812
BstNSI	RCATGY	2 cut(s)	389, 2189
BstSCI	CCNGG	4 cut(s)	402, 603, 1436, 1810
BstSFI	CTRYAG	1 cut(s)	1575
BstUI	CGCG	1 cut(s)	635
BstV1I	GCAGC	7 cut(s)	797, 1045, 1057, 1519, 1995, 2214, 3201
BstV2I	GAAGAC	4 cut(s)	665, 1610, 2686, 3030
BstX2I	RGATCY	3 cut(s)	205, 294, 2050
BstXI	CCANNNNNNTGG	2 cut(s)	2055, 2120
BstYI	RGATCY	3 cut(s)	205, 294, 2050
Bsu36I	CCTNAGG	1 cut(s)	2492
BsuRI	GGCC	6 cut(s)	402, 1146, 1457, 3038, 3128, 3205
BtgI	CCRYGG	2 cut(s)	2509, 2914
BtrI	CACGTC	1 cut(s)	2281
BtsCI	GGATG	9 cut(s)	412, 547, 736, 1366, 1788, 2311, 2734, 2851, 2858
BtsIMutI	CAGTG	9 cut(s)	79, 657, 1283, 1740, 2094, 2102, 2318, 2723, 3118
BveI	ACCTGC	2 cut(s)	40, 1027
CaiI	CAGNNNCTG	1 cut(s)	2320
CciI	TCATGA	2 cut(s)	1831, 2968
Cfr10I	RCCGGY	1 cut(s)	1705
Cfr13I	GGNCC	2 cut(s)	1897, 3126
CseI	GACGC	2 cut(s)	2786, 3093
CsiI	ACCWGGT	2 cut(s)	603, 1810
Csp6I	GTAC	9 cut(s)	637, 671, 922, 1369, 1808, 1815, 1922, 2516, 3164
CviQI	GTAC	9 cut(s)	637, 671, 922, 1369, 1808, 1815, 1922, 2516, 3164
DraI	TTTAAA	2 cut(s)	1035, 1872
DriI	GACNNNNNGTC	1 cut(s)	2977
Eam1104I	CTCTTC	9 cut(s)	1395, 1488, 1742, 1944, 2361, 3006, 3027, 3051, 3111
Eam1105I	GACNNNNNGTC	1 cut(s)	2977
EarI	CTCTTC	9 cut(s)	1395, 1488, 1742, 1944, 2361, 3006, 3027, 3051, 3111
Ecl136II	GAGCTC	1 cut(s)	459
Eco130I	CCWWGG	5 cut(s)	1893, 1901, 2274, 2509, 3181
Eco24I	GRGCYC	1 cut(s)	461
Eco31I	GGTCTC	1 cut(s)	1313
Eco47I	GGWCC	1 cut(s)	1897
Eco53kI	GAGCTC	1 cut(s)	459
Eco57I	CTGAAG	6 cut(s)	85, 522, 863, 1868, 2169, 2913
Eco81I	CCTNAGG	1 cut(s)	2492
Eco88I	CYCGRG	1 cut(s)	3159
EcoICRI	GAGCTC	1 cut(s)	459
EcoO109I	RGGNCCY	1 cut(s)	1897
EcoRII	CCWGG	3 cut(s)	402, 603, 1810
EcoT14I	CCWWGG	5 cut(s)	1893, 1901, 2274, 2509, 3181
EcoT22I	ATGCAT	2 cut(s)	387, 2191
EcoT38I	GRGCYC	1 cut(s)	461
ErhI	CCWWGG	5 cut(s)	1893, 1901, 2274, 2509, 3181
FalI	AAGNNNNNCTT	6 cut(s)	495, 527, 1506, 1538, 1972, 2004
FaqI	GGGAC	1 cut(s)	2528
FauI	CCCGC	1 cut(s)	1535
FauNDI	CATATG	2 cut(s)	619, 991
FbaI	TGATCA	1 cut(s)	2482
Fnu4HI	GCNGC	8 cut(s)	633, 786, 1059, 1071, 1508, 2009, 2228, 3190
FokI	GGATG	9 cut(s)	419, 554, 743, 1373, 1775, 2318, 2741, 2858, 2865
FriOI	GRGCYC	1 cut(s)	461
Fsp4HI	GCNGC	8 cut(s)	633, 786, 1059, 1071, 1508, 2009, 2228, 3190
FspBI	CTAG	2 cut(s)	891, 1551
GluI	GCNGC	8 cut(s)	633, 786, 1059, 1071, 1508, 2009, 2228, 3190
GsaI	CCCAGC	1 cut(s)	1602
GsuI	CTGGAG	1 cut(s)	1083
HaeIII	GGCC	6 cut(s)	402, 1146, 1457, 3038, 3128, 3205
HapII	CCGG	5 cut(s)	1437, 1706, 1777, 2128, 3018
HgaI	GACGC	2 cut(s)	2786, 3093
Hin1I	GRCGYC	1 cut(s)	2797
HincII	GTYRAC	2 cut(s)	1678, 1863
HindII	GTYRAC	2 cut(s)	1678, 1863
HindIII	AAGCTT	1 cut(s)	677
HpaII	CCGG	5 cut(s)	1437, 1706, 1777, 2128, 3018
HphI	GGTGA	4 cut(s)	173, 784, 1625, 2311
Hpy166II	GTNNAC	4 cut(s)	1678, 1863, 2425, 2620
Hpy8I	GTNNAC	4 cut(s)	1678, 1863, 2425, 2620
HpyCH4III	ACNGT	8 cut(s)	1287, 1303, 1609, 1744, 2583, 2665, 2915, 3113
HpyCH4IV	ACGT	5 cut(s)	13, 1221, 2155, 2280, 2374
HpyF10VI	GCNNNNNNNGC	9 cut(s)	359, 446, 494, 1067, 1442, 1504, 1523, 1982, 3080
HpySE526I	ACGT	5 cut(s)	13, 1221, 2155, 2280, 2374
Hsp92I	GRCGYC	1 cut(s)	2797
Kpn2I	TCCGGA	1 cut(s)	3017
KpnI	GGTACC	2 cut(s)	674, 1818
Ksp22I	TGATCA	1 cut(s)	2482
LguI	GCTCTTC	3 cut(s)	1395, 1488, 2361
LmnI	GCTCC	8 cut(s)	464, 472, 502, 754, 1102, 1657, 2021, 2497
Lsp1109I	GCAGC	7 cut(s)	797, 1045, 1057, 1519, 1995, 2214, 3201
LweI	GCATC	6 cut(s)	160, 372, 454, 532, 955, 1825
MabI	ACCWGGT	2 cut(s)	603, 1810
MaeI	CTAG	2 cut(s)	891, 1551
MaeII	ACGT	5 cut(s)	13, 1221, 2155, 2280, 2374
MaeIII	GTNAC	2 cut(s)	565, 1993
MflI	RGATCY	3 cut(s)	205, 294, 2050
MhlI	GDGCHC	1 cut(s)	461
MlyI	GAGTC	8 cut(s)	878, 1169, 1973, 2915, 2987, 2999, 3020, 3023
MmeI	TCCRAC	6 cut(s)	457, 892, 2916, 2922, 2973, 3042
Mph1103I	ATGCAT	2 cut(s)	387, 2191
MroI	TCCGGA	1 cut(s)	3017
MroXI	GAANNNNTTC	1 cut(s)	2704
MslI	CAYNNNNRTG	3 cut(s)	147, 1911, 2118
MspA1I	CMGCKG	2 cut(s)	1130, 2204
MspI	CCGG	5 cut(s)	1437, 1706, 1777, 2128, 3018
MspR9I	CCNGG	4 cut(s)	404, 605, 1438, 1812
Mva1269I	GAATGC	1 cut(s)	3106
MvaI	CCWGG	3 cut(s)	404, 605, 1812
MvnI	CGCG	1 cut(s)	635
MwoI	GCNNNNNNNGC	9 cut(s)	359, 446, 494, 1067, 1442, 1504, 1523, 1982, 3080
NciI	CCSGG	1 cut(s)	1438
NcoI	CCATGG	1 cut(s)	2509
NdeI	CATATG	2 cut(s)	619, 991
NmeAIII	GCCGAG	1 cut(s)	2510
NmuCI	GTSAC	2 cut(s)	565, 1993
NsiI	ATGCAT	2 cut(s)	387, 2191
NspI	RCATGY	2 cut(s)	389, 2189
PaeR7I	CTCGAG	1 cut(s)	3159
PagI	TCATGA	2 cut(s)	1831, 2968
PciSI	GCTCTTC	3 cut(s)	1395, 1488, 2361
PctI	GAATGC	1 cut(s)	3106
PdmI	GAANNNNTTC	1 cut(s)	2704
PkrI	GCNGC	8 cut(s)	634, 787, 1060, 1072, 1509, 2010, 2229, 3191
PleI	GAGTC	8 cut(s)	878, 1168, 1972, 2914, 2986, 2998, 3020, 3022
PpsI	GAGTC	8 cut(s)	878, 1168, 1972, 2914, 2986, 2998, 3020, 3022
PpuMI	RGGWCCY	1 cut(s)	1897
PshBI	ATTAAT	1 cut(s)	2400
Psp124BI	GAGCTC	1 cut(s)	461
Psp1406I	AACGTT	1 cut(s)	1221
Psp5II	RGGWCCY	1 cut(s)	1897
Psp6I	CCWGG	3 cut(s)	402, 603, 1810
PspFI	CCCAGC	1 cut(s)	1598
PspGI	CCWGG	3 cut(s)	402, 603, 1810
PspPI	GGNCC	2 cut(s)	1897, 3126
PspPPI	RGGWCCY	1 cut(s)	1897
PspXI	VCTCGAGB	1 cut(s)	3159
PstNI	CAGNNNCTG	1 cut(s)	2320
PsuI	RGATCY	3 cut(s)	205, 294, 2050
PvuII	CAGCTG	2 cut(s)	1130, 2204
RsaI	GTAC	9 cut(s)	638, 672, 923, 1370, 1809, 1816, 1923, 2517, 3165
RsaNI	GTAC	9 cut(s)	637, 671, 922, 1369, 1808, 1815, 1922, 2516, 3164
RseI	CAYNNNNRTG	3 cut(s)	147, 1911, 2118
SacI	GAGCTC	1 cut(s)	461
SapI	GCTCTTC	3 cut(s)	1395, 1488, 2361
SatI	GCNGC	8 cut(s)	633, 786, 1059, 1071, 1508, 2009, 2228, 3190
Sau96I	GGNCC	2 cut(s)	1897, 3126
SchI	GAGTC	8 cut(s)	878, 1169, 1973, 2915, 2987, 2999, 3020, 3023
ScrFI	CCNGG	4 cut(s)	404, 605, 1438, 1812
SduI	GDGCHC	1 cut(s)	461
SexAI	ACCWGGT	2 cut(s)	603, 1810
SfaNI	GCATC	6 cut(s)	160, 372, 454, 532, 955, 1825
SfcI	CTRYAG	1 cut(s)	1575
Sfr274I	CTCGAG	1 cut(s)	3159
SinI	GGWCC	1 cut(s)	1897
SlaI	CTCGAG	1 cut(s)	3159
SmiMI	CAYNNNNRTG	3 cut(s)	147, 1911, 2118
SmlI	CTYRAG	8 cut(s)	454, 680, 1011, 1957, 2474, 2624, 2762, 3159
SmoI	CTYRAG	8 cut(s)	454, 680, 1011, 1957, 2474, 2624, 2762, 3159
SpeI	ACTAGT	1 cut(s)	890
SsiI	CCGC	6 cut(s)	57, 109, 211, 633, 1528, 2294
SspI	AATATT	1 cut(s)	943
SspMI	CTAG	2 cut(s)	891, 1551
SstI	GAGCTC	1 cut(s)	461
StyD4I	CCNGG	4 cut(s)	402, 603, 1436, 1810
StyI	CCWWGG	5 cut(s)	1893, 1901, 2274, 2509, 3181
TaaI	ACNGT	8 cut(s)	1287, 1303, 1609, 1744, 2583, 2665, 2915, 3113
TaiI	ACGT	5 cut(s)	16, 1224, 2158, 2283, 2377
TaqI	TCGA	7 cut(s)	436, 949, 1192, 2160, 2361, 2717, 3160
TatI	WGTACW	1 cut(s)	1921
TauI	GCSGC	1 cut(s)	635
TscAI	CASTG	9 cut(s)	79, 657, 1290, 1747, 2101, 2109, 2325, 2730, 3118
TseFI	GTSAC	2 cut(s)	565, 1993
TseI	GCWGC	7 cut(s)	785, 1058, 1070, 1507, 2008, 2227, 3189
Tsp45I	GTSAC	2 cut(s)	565, 1993
TspGWI	ACGGA	3 cut(s)	752, 1781, 1877
TspRI	CASTG	9 cut(s)	79, 657, 1290, 1747, 2101, 2109, 2325, 2730, 3118
VpaK11BI	GGWCC	1 cut(s)	1897
VspI	ATTAAT	1 cut(s)	2400
XapI	RAATTY	9 cut(s)	196, 708, 833, 970, 1151, 2013, 2027, 2858, 3209
XceI	RCATGY	2 cut(s)	389, 2189
XcmI	CCANNNNNNNNNTGG	1 cut(s)	410
XhoI	CTCGAG	1 cut(s)	3159
XmnI	GAANNNNTTC	1 cut(s)	2704
XspI	CTAG	2 cut(s)	891, 1551
Zsp2I	ATGCAT	2 cut(s)	387, 2191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.